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近年来,男性不育问题日益突出,其中精索静脉曲张(varicocele, VC)所致不育症的概率为35%~40%,已成为最常见的病因之一[1]。目前对VC致男性不育的机制尚未明确,可能是由于氧化应激作用、血管活性物质的释放、睾丸微循环改变所致的缺氧等多种因素综合作用。近年来研究[2]发现,活性氧自由基(reactive oxygen species,ROS)作为氧化应激的主要产物,可以参与破坏细胞氧化/还原平衡,引起脂质过氧化损伤精子膜,进而导致不育。8-羟基脱氧鸟苷(8-hydroxy-2′-deoxyguanosine, 8-OHdG)作为脂质过氧化反应的产物,也是活性氧引起DNA氧化损伤最主要的标志物之一。它在体内十分稳定,一旦生成就不再被人体进一步代谢,其含量的高低从侧面反映了体内氧化应激水平的高低[3]。故本文旨在探讨VC病人在精索静脉高位结扎术前后体内8-OHdG水平变化情况,进而为结合临床进行分析,现作报道。
精索静脉曲张病人血清8-羟基脱氧鸟苷水平的临床价值
Clinical significance of the serum level change of 8-hydroxy-2'-deoxyguanosine in patients with varicocele
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摘要:
目的探讨精索静脉曲张(varicocele,VC)病人血清8-羟基脱氧鸟苷(8-hydroxy-2'-deoxyguanosine,8-OHdG)水平的临床价值。 方法选取行精索静脉高位结扎术治疗的VC病人30例,测量术前和术后3个月的血清8-OHdG水平,比较手术前后的差异,并分析其水平与单、双侧发病、精索静脉宽度的相关。 结果术前,VC单侧组与双侧组血清8-OHdG水平差异无统计学意义(P>0.05),不同精索静脉宽度病人血清8-OHdG水平差异无统计学意义(P>0.05)。30例病人术后血清8-OHdG水平为(261.64±82.55)pg/mL,较术前的(327.20±101.86)pg/mL明显降低(P < 0.01)。 结论作为一项氧化应激的指标,血液中8-OHdG水平的升高与VC导致的不育相关,且其水平的降低对于行手术治疗的VC病人预后的判断,有一定的临床意义。 Abstract:ObjectiveTo investigate the clinical value of the serum level change of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in varicocele (VC) patients. MethodsThe serum levels of 8-OHdG in 30 VC patients treated with high spermatic vein ligation were measured before and after 3 months of surgery.The differences of the serum levels of 8-OHdG between before and after surgery were compared, and the correlations of the level of 8-OHdG with incidence rate of single or bilateral VC and width of spermatic vein were analyzed. ResultsBefore surgery, there was no statistically significant in the serum level of 8-OHdG between the VC unilateral group and bilateral group (P>0.05), and there was no statistical significance in the serum levels of 8-OHdG level among patients with different widths of spermatic vein (P>0.05).The postoperative 8-OHdG level[(261.64±82.55) pg/mL] was significantly lower than that of preoperative level[(327.20±101.86) pg/mL] in 30 patients (P < 0.01). ConclusionsThe serum level of 8-OHdG increasing, an indicator of oxidative stress, is related to the infertility caused by VC, and the level of 8-OHdG decreasing has a certain clinical significance in judging the prognosis of VC patients treated with operation. -
Key words:
- varicocele /
- 8-hydroxy-2'-deoxyguanosine /
- oxidative stress
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