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胃癌是临床上高发的的恶性肿瘤之一,其发病率高居恶性肿瘤的第四位,尤其我国为胃癌的高发地区,给我国带来巨大的经济和社会压力[1-2]。目前胃癌的发病机制尚不清楚,常规的化疗和生物治疗效果不理想,因此对胃癌发病机制的研究以及临床治疗成为学界关注的焦点[3-5]。近年来发现,电压门控性钾离子通道与癌症的发病关系密切,人类eag相关基因(HERG)蛋白则是构成延迟整流性钾通道的α亚单位,且在多种恶性肿瘤可以检测到其阳性表达,成为人们研究和治疗胃癌的新热点[6-8]。因此, 本文通过研究HERG蛋白在胃癌组织中表达,探讨其与胃癌病理学特征的相关性,为临床的治疗提供依据。
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胃癌组织HERG蛋白阳性表达率76.92%(60/78),明显高于癌旁组织(0/78),差异有统计学意义(χ2=20.35, P<0.01)(见图 1)。
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HERG蛋白阳性表达率在胃癌病人年龄、性别、肿瘤浸润、TNM分期及肿瘤大小方面差异无统计学意义(P>0.05),与分化程度和淋巴结转移有关(P<0.05和P<0.01)(见表 1)。
临床病理特征 n HERG蛋白
阳性表达χ2 P 年龄/岁 ≤50 38 31(81.58) 0.91 >0.05 >50 40 29(72.50) 性别 男 41 32(78.05) 0.06 >0.05 女 37 28(75.68) 肿瘤浸润 侵及浆膜 51 40(78.43) 0.19 >0.05 未侵及浆膜 21 20(74.07) TNM分期 Ⅰ期 21 14(66.67) 3.81 >0.05 Ⅱ期 35 26(74.29) Ⅲ期 22 20(90.91) 肿瘤直径/cm <5 51 41(80.39) 1.00 >0.05 ≥5 27 19(70.37) 分化程度 高分化 33 21(63.64) 5.69 <0.05 中低分化 45 39(86.67) 淋巴结转移 有 42 39(92.86) 13.02 <0.01 无 36 21(58.33) 表 1 胃癌组织HERG蛋白表达与临床病理特征关系[n; 百分率(%)]
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HERG蛋白阳性表达病人中位总生存时间和无进展生存期分别为23个月和20个月,HERG蛋白阴性表达病人中位总生存时间和无进展生存期分别为30个月和24个月,经Log-rank检验比较,HERG蛋白阳性表达病人和阴性表达病人中位总生存时间(χ2=0.11,P>0.05)和无进展生存期(χ2=0.50,P>0.05),差异无统计学意义(见图 2)。
HERG蛋白在胃癌组织中的表达特点及与病理学特征的关系
Expression characteristics of HERG protein in gastric cancer tissue and its correlation with pathological features
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摘要:
目的探讨人类eag相关基因(HERG)蛋白表达与胃癌病理学特征的相关性。 方法选取2014年2月至2017年3月病理科保存的胃癌组织标本78例,同时选取同癌旁正常组织(距癌切缘>5 cm)作为对照,采用免疫组化法检测HERG蛋白表达。 结果胃癌组织HERG蛋白阳性表达率为76.92%,明显高于癌旁组织的0.00%(χ2=20.35,P < 0.01);中低分化、有淋巴结转移病人HERG蛋白阳性表达率分别为86.67%和92.86%,高于高分化、无淋巴结转移病人的63.64%和58.33%(P < 0.05和P < 0.01);HERG蛋白阳性表达与胃癌病人年龄、性别、肿瘤浸润、TNM分期及肿瘤大小无明显相关性(P>0.05);HERG蛋白阳性表达病人中位总生存时间和和无进展生存期为23个月和20个月,HERG蛋白阴性表达病人中位总生存时间和无进展生存期为30个月和24个月,差异无统计学意义(P>0.05)。 结论HERG蛋白表达与胃癌分化程度、淋巴结转移有关,与病人预后关系不明确。 Abstract:ObjectiveTo investigate the expression characteristics of human eag-related gene (HERG) protein in gastric cancer tissue, and its correlation with pathological features. MethodsThe expression levels of HERG protein in 78 gastric cancer tissue specimens and normal tissue adjacent to the cancer (from the margin of cancer >5 cm) from February 2014 to March 2017.were detected using immunohistochemistry ResultsThe positive rate of HERG protein expression in gastric cancer was 76.92%, which was significantly higher than that in adjacent tissue(0.00%) (P < 0.01).The positive rates of HERG protein expression in patients with low-middle differentiation and lymph node metastasis were 86.67% and 92.86%, which were significantly higher than those in patients with high differentiation and no lymph node metastasis (63.64% and 58.33%), respectively(P < 0.05 and P < 0.01).The positive expression rates of HERG protein were not significantly correlated with age, sex, tumor infiltration, TNM staging and tumor size in gastric cancer patients(P>0.05).The median survival time and progression-free survival time in positive HERG patients were 23 months and 20 months, respectively, the median survival time and progression-free survival time in patients with negative HERG were 30 months and 24 months, respectively, and the difference of which was not statistically significant(P>0.05). ConclusionsThe expression of HERG protein is related to the degree of differentiation and lymph node metastasis of gastric cancer, but it is not clear with the prognosis of the patients. -
Key words:
- stomach neoplasms /
- human eag related gene /
- clinical pathology /
- prognosis
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表 1 胃癌组织HERG蛋白表达与临床病理特征关系[n; 百分率(%)]
临床病理特征 n HERG蛋白
阳性表达χ2 P 年龄/岁 ≤50 38 31(81.58) 0.91 >0.05 >50 40 29(72.50) 性别 男 41 32(78.05) 0.06 >0.05 女 37 28(75.68) 肿瘤浸润 侵及浆膜 51 40(78.43) 0.19 >0.05 未侵及浆膜 21 20(74.07) TNM分期 Ⅰ期 21 14(66.67) 3.81 >0.05 Ⅱ期 35 26(74.29) Ⅲ期 22 20(90.91) 肿瘤直径/cm <5 51 41(80.39) 1.00 >0.05 ≥5 27 19(70.37) 分化程度 高分化 33 21(63.64) 5.69 <0.05 中低分化 45 39(86.67) 淋巴结转移 有 42 39(92.86) 13.02 <0.01 无 36 21(58.33) -
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