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观察组血清NSE、TNF-α、IL-6水平均明显高于对照组(P < 0.01)。轻度疼痛组、中度疼痛组、重度疼痛组间血清NSE、TNF-α、IL-6水平逐渐升高(P < 0.05)(见表 1)。
分组 n NSE/(μg/L) TNF-α/(pg/mL) IL-6/(pg/mL) 对照组 30 9.87±0.65 92.75±6.18 45.66±3.04 观察组 66 13.68±0.92 185.44±12.36 75.67±5.07 t — 20.45 38.85 30.01 P — < 0.01 < 0.01 < 0.01 轻度疼痛组 23 10.45±0.67 153.94±10.26 55.98±3.72 中度疼痛组 25 14.02±0.93* 179.11±11.94* 79.19±5.27* 重度疼痛组 18 15.25±1.01*# 197.99±13.19*# 86.01±5.74*# F — 174.57 72.90 219.46 P — < 0.01 < 0.01 < 0.01 MS组内 — 0.762 138.016 24.303 q检验:与轻度疼痛组比较*P < 0.05;与中度疼痛组比较#P < 0.05 表 1 各组血清NSE、TNF-α、IL-6水平比较(x±s)
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观察组静脉血中CD3+、CD4+、CD8+T淋巴细胞比率均明显低于对照组(P < 0.01),CD4+/CD8+比值明显高于对照组(P < 0.01)。轻度疼痛组、中度疼痛组、重度疼痛组CD3+、CD4+、CD8+T淋巴细胞比率及CD4+/CD8+比值间差异均有统计学意义(P < 0.01)(见表 2)。
分组 n CD3+/% CD4+/% CD8+/% CD4+/CD8+ 对照组 30 67.23±4.42 37.56±2.54 28.64±1.93 1.37±0.09 观察组 66 56.64±3.76 30.47±2.03 21.33±1.42 1.46±0.07 t — 12.10 14.64 20.82 5.33 P — < 0.01 < 0.01 < 0.01 < 0.01 轻度疼痛组 23 56.56±3.77 34.44±2.26 23.66±1.53 1.45±0.11 中度疼痛组 25 53.95±3.59* 30.06±2.04* 21.21±1.44* 1.41±0.09 重度疼痛组 18 52.05±3.47* 28.28±1.83*# 20.47±1.36* 1.33±0.08*# F — 8.06 49.94 28.45 8.18 P — < 0.01 < 0.01 < 0.01 < 0.01 MS组内 — 13.122 4.273 2.107 0.009 q检验:与轻度疼痛组比较*P < 0.05;与中度疼痛组比较#P < 0.05 表 2 各组T细胞亚群水平比较(x±s)
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带状疱疹病人VAS评分与血清NSE、TNF-α、IL-6水平均呈正相关关系(r=0.695、0.718、0.726,P < 0.05),与CD3+、CD4+、CD8+T淋巴细胞比率及CD4+/CD8+比值均呈负相关关系(r=-0.624、-0.674、-0.524、-0.445,P < 0.05)。
带状疱疹病人血清NSE、T细胞亚群、炎性细胞因子变化与疼痛的相关性研究
Correlation between changes of serum NSE, T cell subsets, inflammatory cytokines and pain in patients with herpes zoster
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摘要:
目的探讨带状疱疹病人血清神经元特异性烯醇化酶(NSE)、T细胞亚群、炎性细胞因子变化与疼痛的相关性。 方法将66例急性期带状疱疹病人设为观察组,根据视觉模拟评分法(VAS)分为轻度疼痛组、中度疼痛组、重度疼痛组。另选取同期健康体检者30名设为对照组。比较各组血清NSE、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,静脉血中CD3+、CD4+、CD8+T淋巴细胞比率及CD4+/CD8+比值。分析观察组以上各指标与VAS评分的相关性。 结果观察组血清NSE、TNF-α、IL-6水平均明显高于对照组(P < 0.01)。轻度疼痛组、中度疼痛组、重度疼痛组间血清NSE、TNF-α、IL-6水平逐渐升高(P < 0.05)。观察组静脉血中CD3+、CD4+、CD8+T淋巴细胞比率均明显低于对照组(P < 0.01),CD4+/CD8+比值明显高于对照组(P < 0.01)。轻度疼痛组、中度疼痛组、重度疼痛组CD3+、CD4+、CD8+T淋巴细胞比率及CD4+/CD8+比值间差异均有统计学意义(P < 0.01)。观察组病人VAS评分与血清NSE、TNF-α、IL-6水平均呈正相关关系(r=0.695、0.718、0.726,P < 0.05),与CD3+、CD4+、CD8+T淋巴细胞比率及CD4+/CD8+比值均呈负相关关系(r=-0.624、-0.674、-0.524、-0.445,P < 0.05)。 结论带状疱疹病人存在免疫功能低下、炎症反应程度增加和一定程度的神经损伤情况,与疼痛的发生密切相关。 -
关键词:
- 带状疱疹 /
- 疼痛 /
- 神经元特异性烯醇化酶 /
- T细胞亚群 /
- 炎症反应
Abstract:ObjectiveTo investigate the correlation between the changes of serum neuron-specific enolase (NSE), T cell subsets, inflammatory cytokines and pain in patients with herpes zoster. MethodsA total of 66 patients with acute herpes zoster were set as the observation group, which were divided into mild pain group, moderate pain group and severe pain group according to visual analogue scale(VAS) score.Another 30 healthy people in the same period were set as the control group.The serum levels of NSE, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and the ratios of CD3+, CD4+, CD8+ T lymphocytes and CD4+/CD8+ in venous blood were compared among the groups.The correlation between the above indexes and VAS score in the observation group was analyzed. ResultsThe serum levels of NSE and TNF-α in observation group were significantly higher than those in control group (P < 0.01).The serum levels of NSE, TNF-α, IL-6 increased gradually among mild pain group, moderate pain group and severe pain group (P < 0.05).The ratios of CD3+, CD4+, CD8+ T lymphocytes in venous blood in observation group were significantly lower than those in control group(P < 0.01), and the ratio of CD4+/CD8+ was markedly higher than that in control group (P < 0.01).The ratios of CD3+, CD4+, CD8+ T lymphocytes and CD4+/CD8+ decreased gradually among mild pain group, moderate pain group and severe pain group (P < 0.01).The VAS score in observation group was positively correlated with the serum levels of NSE, TNF-α and IL-6(r=0.695, 0.718, 0.726, P < 0.05), and negatively correlated with the ratios of CD3+, CD4+, CD8+ T lymphocytes and CD4+/CD8+ in venous blood (r=-0.624, -0.674, -0.524, -0.445, P < 0.05). ConclusionsPatients with herpes zoster have low immune function, increased inflammatory reaction and certain degree of nerve injury, which are closely related to the occurrence of pain. -
Key words:
- herpes zoster /
- pain /
- neuron-specific enolase /
- T cell subsets /
- inflammatory reaction
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表 1 各组血清NSE、TNF-α、IL-6水平比较(x±s)
分组 n NSE/(μg/L) TNF-α/(pg/mL) IL-6/(pg/mL) 对照组 30 9.87±0.65 92.75±6.18 45.66±3.04 观察组 66 13.68±0.92 185.44±12.36 75.67±5.07 t — 20.45 38.85 30.01 P — < 0.01 < 0.01 < 0.01 轻度疼痛组 23 10.45±0.67 153.94±10.26 55.98±3.72 中度疼痛组 25 14.02±0.93* 179.11±11.94* 79.19±5.27* 重度疼痛组 18 15.25±1.01*# 197.99±13.19*# 86.01±5.74*# F — 174.57 72.90 219.46 P — < 0.01 < 0.01 < 0.01 MS组内 — 0.762 138.016 24.303 q检验:与轻度疼痛组比较*P < 0.05;与中度疼痛组比较#P < 0.05 表 2 各组T细胞亚群水平比较(x±s)
分组 n CD3+/% CD4+/% CD8+/% CD4+/CD8+ 对照组 30 67.23±4.42 37.56±2.54 28.64±1.93 1.37±0.09 观察组 66 56.64±3.76 30.47±2.03 21.33±1.42 1.46±0.07 t — 12.10 14.64 20.82 5.33 P — < 0.01 < 0.01 < 0.01 < 0.01 轻度疼痛组 23 56.56±3.77 34.44±2.26 23.66±1.53 1.45±0.11 中度疼痛组 25 53.95±3.59* 30.06±2.04* 21.21±1.44* 1.41±0.09 重度疼痛组 18 52.05±3.47* 28.28±1.83*# 20.47±1.36* 1.33±0.08*# F — 8.06 49.94 28.45 8.18 P — < 0.01 < 0.01 < 0.01 < 0.01 MS组内 — 13.122 4.273 2.107 0.009 q检验:与轻度疼痛组比较*P < 0.05;与中度疼痛组比较#P < 0.05 -
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