耐亚胺培南铜绿假单胞菌的耐药性及超广谱-内酰胺酶的基因分析

张伦军; 丁晓琳; 郭普; 钟政荣; 沈继龙

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耐亚胺培南铜绿假单胞菌的耐药性及超广谱-内酰胺酶的基因分析

张伦军 , 丁晓琳 , 郭普 , 钟政荣 , 沈继龙

文章导航 > 蚌埠医学院学报 > 2013, 37 > 10: (1328-1330)

引用本文:

Citation:

耐亚胺培南铜绿假单胞菌的耐药性及超广谱-内酰胺酶的基因分析

1.
1. 安徽医科大学病原生物学教研室，安徽合肥230032;
2.
2. 蚌埠医学院第一附属医院检验科，安徽蚌埠233004

作者简介: 张伦军(1980-) ，男，主管检验师.

通讯作者: 沈继龙, shenjilong53@126.com

基金项目:

The antibiotic-resistance and its gene analysis of extended-spectrum -lactamases in imipenem-resistant Pseudomonas aeruginosa

1.
1. Department of Pathogen Biology，Anhui Medical University，Hefei Anhui 230032;
2.
2. Department of Laboratory Medicine，The First Affiliated Hospital of Bengbu Medical College，Bengbu Anhui 233004，China

Corresponding author: SHEN Ji-long, shenjilong53@126.com

摘要: 目的:探讨临床分离铜绿假单胞菌的药敏谱及其产超广谱-内酰胺酶(ESBLs)的情况、基因分布和流行特性。方法:分析208株铜绿假单胞菌对16种抗生素的药敏谱,筛选耐亚胺培南铜绿假单胞菌,采用PCr检测ESBLs基因(TEM、SHV、OXA-10、OXA-2、PEr、VEB)。结果:53株对亚胺培南耐药,耐药率为25.48%(53/208),且对头孢替坦(CTE)、头孢唑林(CZ)、氨苄西林/舒巴坦(AMS)、呋喃妥因(FTN)等耐药率较高;53株亚胺培南耐药菌经PCr检测,产ESBLs菌株23株,其中TEM型16株,OXA-10型4株,PEr型3株,未检测出OXA-2和SHV型。结论:耐亚胺培南铜绿假单胞菌中产ESBLs菌株的基因型主要为TEM型,且为多重耐药,临床应结合药敏谱,合理使用抗生素。
- 铜绿假单胞菌 /
- -内酰胺酶 /
- 耐药性
Abstract: Objective: To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa and extended-spectrum -lactamase (ESBLs) producing condition, gene distribution and epidemiological characteristics in clinic. Methods: The antimicrobial susceptibility was detected with the Vitek-2 compact automatic bacteria identification system, 16 antibiotics for 208 Pseudomonas aeruginosa isolated. The imipenem-resistant Pseudomonas aeruginosa were selected. ESBLs genes produced by imipenem resistance were detected by PCr, the ESBLs genes contain TEM，SHV，OXA-10，OXA-2，PEr and VEB. results: In the 208 isolates，53 strains of Pseudomonas aeruginosa were resistant to imipenem, the drug resistance rate was 25. 48%. The isolates had a high rate of drug-resistance against CTE，CZ，AMS and FTN; The PCr results showed that in the 53 imipenem-resistant Pseudomonas aeruginosa，ESBLs gene were produced in 23 strains, 16 strains were TEM，4 for OXA-10 and 3 for PEr gene; OXA-2，SHV was not detected. Conclusions: TEM of Extended-spectrum-lactamase producing in imipenem-resistant Pseudomonas aeruginosa is the main gene in our hospital，most of them are multi-drug-resistance，detecting the antimicrobial susceptibility and using antibiotics rationally is essential in clinic.
- Pseudomonas aeruginosa /
- -lactamase /
- drug-resistance

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耐亚胺培南铜绿假单胞菌的耐药性及超广谱-内酰胺酶的基因分析

通讯作者: 沈继龙, shenjilong53@126.com

作者简介: 张伦军(1980-) ，男，主管检验师.

1. 1. 安徽医科大学病原生物学教研室，安徽合肥230032;
2. 2. 蚌埠医学院第一附属医院检验科，安徽蚌埠233004

收稿日期: 2013-01-25
网络出版日期: 2013-10-15

基金项目:

关键词:

摘要: 目的:探讨临床分离铜绿假单胞菌的药敏谱及其产超广谱-内酰胺酶(ESBLs)的情况、基因分布和流行特性。方法:分析208株铜绿假单胞菌对16种抗生素的药敏谱,筛选耐亚胺培南铜绿假单胞菌,采用PCr检测ESBLs基因(TEM、SHV、OXA-10、OXA-2、PEr、VEB)。结果:53株对亚胺培南耐药,耐药率为25.48%(53/208),且对头孢替坦(CTE)、头孢唑林(CZ)、氨苄西林/舒巴坦(AMS)、呋喃妥因(FTN)等耐药率较高;53株亚胺培南耐药菌经PCr检测,产ESBLs菌株23株,其中TEM型16株,OXA-10型4株,PEr型3株,未检测出OXA-2和SHV型。结论:耐亚胺培南铜绿假单胞菌中产ESBLs菌株的基因型主要为TEM型,且为多重耐药,临床应结合药敏谱,合理使用抗生素。

English Abstract

The antibiotic-resistance and its gene analysis of extended-spectrum -lactamases in imipenem-resistant Pseudomonas aeruginosa

Corresponding author: SHEN Ji-long, shenjilong53@126.com

1. 1. Department of Pathogen Biology，Anhui Medical University，Hefei Anhui 230032;
2. 2. Department of Laboratory Medicine，The First Affiliated Hospital of Bengbu Medical College，Bengbu Anhui 233004，China

Received Date: 2013-01-25
Available Online: 2013-10-15

Abstract: Objective: To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa and extended-spectrum -lactamase (ESBLs) producing condition, gene distribution and epidemiological characteristics in clinic. Methods: The antimicrobial susceptibility was detected with the Vitek-2 compact automatic bacteria identification system, 16 antibiotics for 208 Pseudomonas aeruginosa isolated. The imipenem-resistant Pseudomonas aeruginosa were selected. ESBLs genes produced by imipenem resistance were detected by PCr, the ESBLs genes contain TEM，SHV，OXA-10，OXA-2，PEr and VEB. results: In the 208 isolates，53 strains of Pseudomonas aeruginosa were resistant to imipenem, the drug resistance rate was 25. 48%. The isolates had a high rate of drug-resistance against CTE，CZ，AMS and FTN; The PCr results showed that in the 53 imipenem-resistant Pseudomonas aeruginosa，ESBLs gene were produced in 23 strains, 16 strains were TEM，4 for OXA-10 and 3 for PEr gene; OXA-2，SHV was not detected. Conclusions: TEM of Extended-spectrum-lactamase producing in imipenem-resistant Pseudomonas aeruginosa is the main gene in our hospital，most of them are multi-drug-resistance，detecting the antimicrobial susceptibility and using antibiotics rationally is essential in clinic.