Objective: To explore the appliciation effects of PDCA circulation method in managing the cleaned and disinfected laryngoscope room. Methods: PDCA circulation method was used to collect material, find problem，analyze the reason，make plan, organize and implement and evaluate the effects. The biology condition of 55 cleaned and disinfected laryngoscopes, 50 disinfectants in use, 56 cleaned hands of staff and 50 times of disinfected laryngoscope store room atmosphere were randomly measured before and after using PDCA circulation method. results: The quality of the cleaned and disinfected laryngoscope，disinfectant in use, the cleaned hand of staff and the atmosphere of disinfected laryngoscope store room met the requirement of Technical Specifications of Endoscope Decontamination and Disinfection after using the PDCA circulation method, the total qualified rate of which was 99. 05%. The qualified rate differences of four indexes after using the PDCA cycling method were statistically higher than before (P ＜ 0. 05 ) . Conclusions: The effects of PDCA circulation method in managing the cleaned and disinfected are good，which is an effective way to improve the quality of cleaned and disinfected laryngoscope room.