UF-100流式尿沉渣分析仪在筛选妊娠早期无症状尿路感染中的应用

林萍; 孙阳; 张友玲; 李凤云

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UF-100流式尿沉渣分析仪在筛选妊娠早期无症状尿路感染中的应用

林萍 , 孙阳 , 张友玲 , 李凤云

文章导航 > 蚌埠医学院学报 > 2006, 31 > 2: (178-180)

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UF-100流式尿沉渣分析仪在筛选妊娠早期无症状尿路感染中的应用

1. 上海市闵行区中心医院临检室, 201100;
2. 上海市闵行区中心医院细菌室, 201100;
3. 蚌埠医学院病原生物学教研室, 安徽蚌埠 233030

作者简介: 林萍(1968),女,副主任技师.

基金项目:
上海市闵行区科委资助项目(编号03-119)
中图分类号: R691.3

The applying of the flow cytometer UF-IO0 in screening asymptomatic urinary tract infection in early pregnancy

1. Department of Clinical Laboratories, Minghang District Centreal Hospital, Ruijin Hospital Group, Shanghai 201100;
2. Labratory of Bacteria, Minghang District Centreal Hospital, Ruijin Hospital Group, Shanghai 201100;
3. Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu 233030, China
CLC number: R691.3

摘要: 目的: 探讨UF-100流式尿沉渣分析仪在筛选妊娠早期无症状尿路感染(asymp tomatic urinary tract infections,AUTI)中的可靠性。方法: 收取合格中段尿309份,取10 μl做细菌培养及菌落计数,剩余标本用UF-100流式尿沉渣分析仪主要检测细菌计量、白细胞计数。结果: 细菌培养有21份培养出有意义细菌(菌落计数≥ 10⁴~10⁵ CFU/ml),阳性率为6.8%。UF-100分析仪检出32份阳性标本,其中有17例细菌培养阴性,假阳性率为5.9%。6例细菌培养阳性,UF-100分析为阴性,假阴性率为28.6%。UF-100分析仪的敏感性为71.4%,特异性为94.1%,阳性预示值为46.9%。两法检测阳性率差异有显著性(P<0.05),两法检测结果间有密切关联性(P<0.005)。结论: UF-100分析仪可初筛AUTI,诊断AUTI仍以细菌培养为首选。
- 尿路感染 /
- UF-100流式尿沉渣分析仪 /
- 妊娠并发症
Abstract: Objective: To study the reliability of flow cytometer UF-100 in detecting asymptomatic urinary tract infections(AUTI) in early pregnancy.Methods: Three-hundred and nine fresh midstream clean-catch urine sample were collected in accordance with standard guideline.10 μl of cleancatch midstream urine specimen was examined by cultures(colony counts of ≥ 10^[4~5] CFU/ml) the other portion was tested with UF-100 for presence of bacteria count and white blood cell.Results: Twenty-one patient(6.8%) had cultures of 10,000 or more colony-forming units of a uropathogen per milliliter.The sensitivities of the UF-100 test in identifying patients with positive cultures was 71.4%,and the specificities was 94.1%.Six patients(28.6% 6/21) had negative UF-100 screening tests but positive urine cultures,and other seventeen patients had positive UF-100 screening tests but negative urine cultures,The positive predict value(PPV) of urine UF-100 evaluation was 46.9%.There was a significant difference in positive rate between the two methods(P<0.05),but there was a significant correlation in test results between the two methods(P<0.005).Conclusions: Flow cytometer UF-100 tests are not sufficiently sensitive to be of use in the screening for AUTI and therefore many patients would be missed.Bacteriuria culture must be done in diagnosing AUTI.
- urinary tract infections /
- UF-100 uurine flow cytometer /
- pregnance complications

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UF-100流式尿沉渣分析仪在筛选妊娠早期无症状尿路感染中的应用

作者简介: 林萍(1968),女,副主任技师.

1. 上海市闵行区中心医院临检室, 201100;
2. 上海市闵行区中心医院细菌室, 201100;
3. 蚌埠医学院病原生物学教研室, 安徽蚌埠 233030

收稿日期: 2005-04-08

基金项目: 上海市闵行区科委资助项目(编号03-119)

关键词:

摘要: 目的: 探讨UF-100流式尿沉渣分析仪在筛选妊娠早期无症状尿路感染(asymp tomatic urinary tract infections,AUTI)中的可靠性。方法: 收取合格中段尿309份,取10 μl做细菌培养及菌落计数,剩余标本用UF-100流式尿沉渣分析仪主要检测细菌计量、白细胞计数。结果: 细菌培养有21份培养出有意义细菌(菌落计数≥ 10⁴~10⁵ CFU/ml),阳性率为6.8%。UF-100分析仪检出32份阳性标本,其中有17例细菌培养阴性,假阳性率为5.9%。6例细菌培养阳性,UF-100分析为阴性,假阴性率为28.6%。UF-100分析仪的敏感性为71.4%,特异性为94.1%,阳性预示值为46.9%。两法检测阳性率差异有显著性(P<0.05),两法检测结果间有密切关联性(P<0.005)。结论: UF-100分析仪可初筛AUTI,诊断AUTI仍以细菌培养为首选。

English Abstract

The applying of the flow cytometer UF-IO0 in screening asymptomatic urinary tract infection in early pregnancy

1. Department of Clinical Laboratories, Minghang District Centreal Hospital, Ruijin Hospital Group, Shanghai 201100;
2. Labratory of Bacteria, Minghang District Centreal Hospital, Ruijin Hospital Group, Shanghai 201100;
3. Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu 233030, China

Received Date: 2005-04-08

Abstract: Objective: To study the reliability of flow cytometer UF-100 in detecting asymptomatic urinary tract infections(AUTI) in early pregnancy.Methods: Three-hundred and nine fresh midstream clean-catch urine sample were collected in accordance with standard guideline.10 μl of cleancatch midstream urine specimen was examined by cultures(colony counts of ≥ 10^[4~5] CFU/ml) the other portion was tested with UF-100 for presence of bacteria count and white blood cell.Results: Twenty-one patient(6.8%) had cultures of 10,000 or more colony-forming units of a uropathogen per milliliter.The sensitivities of the UF-100 test in identifying patients with positive cultures was 71.4%,and the specificities was 94.1%.Six patients(28.6% 6/21) had negative UF-100 screening tests but positive urine cultures,and other seventeen patients had positive UF-100 screening tests but negative urine cultures,The positive predict value(PPV) of urine UF-100 evaluation was 46.9%.There was a significant difference in positive rate between the two methods(P<0.05),but there was a significant correlation in test results between the two methods(P<0.005).Conclusions: Flow cytometer UF-100 tests are not sufficiently sensitive to be of use in the screening for AUTI and therefore many patients would be missed.Bacteriuria culture must be done in diagnosing AUTI.