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2009 Vol. 34, No. 3
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2009, 34(3): 185-187.
Abstract:
Objective: To study the difference between CD31 and CD34 in showing the microvessel density(MVD) of non-small lung cancer(NSCLC),and to investigate the relationship between MVD and clincopathological parameters.Methods: ElivisionTM plus immunohistochemical techinique was used to detect the expression of CD31 and CD34 in 57 cases of NSCLC.Results: The MVDs shown by CD31 and CD34 were(29.7±12.1) and(38.2±12.7) respectively,within a range of 10-61 and 19-76.The difference was significant(P<0.001),but the correlation was good(P<0.05).No significant difference between MVD and clinopathological parameters was observed(P>0.05).Conclusions: The endothelial cell of micro-vessels can be shown by both CD31 and CD34,but CD34 is better than CD31 and is more suitable for clinical application.There is no significant difference between MVD and clinopathological parameters including metastasis of lymph node.
Objective: To study the difference between CD31 and CD34 in showing the microvessel density(MVD) of non-small lung cancer(NSCLC),and to investigate the relationship between MVD and clincopathological parameters.Methods: ElivisionTM plus immunohistochemical techinique was used to detect the expression of CD31 and CD34 in 57 cases of NSCLC.Results: The MVDs shown by CD31 and CD34 were(29.7±12.1) and(38.2±12.7) respectively,within a range of 10-61 and 19-76.The difference was significant(P<0.001),but the correlation was good(P<0.05).No significant difference between MVD and clinopathological parameters was observed(P>0.05).Conclusions: The endothelial cell of micro-vessels can be shown by both CD31 and CD34,but CD34 is better than CD31 and is more suitable for clinical application.There is no significant difference between MVD and clinopathological parameters including metastasis of lymph node.
2009, 34(3): 188-191.
Abstract:
Objective: To investigate the effects of genistein on homocysteine-mediated endothelial cell toxicity and inflammatory response and the underlying mechanisms of such effects.Methods: MTT assay was used to detect cell viability,changes in the morphology of cells were observed under microscope;ELISA assay was used to examine the levels of IL-6 and ICAM-1.Results: The viability of cells treated with 5.0 mmol/L of Hcy was lower than that of other groups,and the difference was statistics significance compared with the control group(P<0.01).ECV-304 cells were identified by their typical cobblestone morphology when viewed by light microscope in control group,the state of cells was also better,but the arrange of cells became disorderly,congregate with 5.0 mmol/L of Hcy in test groups,and the state was the worst.ELISA showed that homocysteine(5.0 mmol/L) significantly increased the expression of IL-6 and ICAM-1,compared with the control group(P<0.01).However,pretreatment with genistein(10,50,100 μmol/L) reversed the decreased cell viability,improved the state of cells in a large extent,and suppressed an increase of expressions of IL-6 and ICAM-1 of cells induced by homcysteine markedly,especially the 100 μmol/L of genistein(P<0.01). Conclusions: Genistein inhibits homocysteine-induced endothelial dysfunction and inflammatory response obviously.
Objective: To investigate the effects of genistein on homocysteine-mediated endothelial cell toxicity and inflammatory response and the underlying mechanisms of such effects.Methods: MTT assay was used to detect cell viability,changes in the morphology of cells were observed under microscope;ELISA assay was used to examine the levels of IL-6 and ICAM-1.Results: The viability of cells treated with 5.0 mmol/L of Hcy was lower than that of other groups,and the difference was statistics significance compared with the control group(P<0.01).ECV-304 cells were identified by their typical cobblestone morphology when viewed by light microscope in control group,the state of cells was also better,but the arrange of cells became disorderly,congregate with 5.0 mmol/L of Hcy in test groups,and the state was the worst.ELISA showed that homocysteine(5.0 mmol/L) significantly increased the expression of IL-6 and ICAM-1,compared with the control group(P<0.01).However,pretreatment with genistein(10,50,100 μmol/L) reversed the decreased cell viability,improved the state of cells in a large extent,and suppressed an increase of expressions of IL-6 and ICAM-1 of cells induced by homcysteine markedly,especially the 100 μmol/L of genistein(P<0.01). Conclusions: Genistein inhibits homocysteine-induced endothelial dysfunction and inflammatory response obviously.
2009, 34(3): 192-194.
Abstract:
Objective: To investigate the mechanism of attenuad bacillus calmette-guerin(BCG) unite high dose of vitamin A(Vit A) interfered in asthma pneumonia.Methods: All mice were allergized by intraperitoneal injection of ovum albumin(OVA) and two treatment groups were endermic injeced with BCG at 1st day of experiment.The mice of one of treatment groups took orally high dose of vitamin A 100 u after 10 days and other contrasted by NS.All mice atomization OVA were stimulated asthma after experiment.two weeks and were examined with methods pathology,histochemis-try,ELISA.Results: Longer course of severe allergic pneumonia,significant higher IL-13 and TNF-α factors of Th2 reaction in the group of asthma.The mice of group sensitization with OVA and BCG innoculation occurrenced Th1 reaction and Th2 reaction suppressed.Another mice of group with overdose VitA and BCG inoculation aggravated lung lymphoid inflammation.Vit A promoted mononuclear-macrophages shifted into the inflammation site of lung,aggratated alveolitis by oneself lysosomal enzyme release.Down blood plasma IL-13 and TNF-α factors level obviously,and hypolevel IFN-γ.Overdose VitA changed Th1 type induced by BCG inoculation,provocated non-Th1,non-Th2 reaction.Conclusions: BCG unite high dose of vitamin A possible activation lysosomal hydrolase of cell to aggrav pneumonia at acute stage,can provocated non-Th1,non-Th2 reaction and shorten the course of disease.
Objective: To investigate the mechanism of attenuad bacillus calmette-guerin(BCG) unite high dose of vitamin A(Vit A) interfered in asthma pneumonia.Methods: All mice were allergized by intraperitoneal injection of ovum albumin(OVA) and two treatment groups were endermic injeced with BCG at 1st day of experiment.The mice of one of treatment groups took orally high dose of vitamin A 100 u after 10 days and other contrasted by NS.All mice atomization OVA were stimulated asthma after experiment.two weeks and were examined with methods pathology,histochemis-try,ELISA.Results: Longer course of severe allergic pneumonia,significant higher IL-13 and TNF-α factors of Th2 reaction in the group of asthma.The mice of group sensitization with OVA and BCG innoculation occurrenced Th1 reaction and Th2 reaction suppressed.Another mice of group with overdose VitA and BCG inoculation aggravated lung lymphoid inflammation.Vit A promoted mononuclear-macrophages shifted into the inflammation site of lung,aggratated alveolitis by oneself lysosomal enzyme release.Down blood plasma IL-13 and TNF-α factors level obviously,and hypolevel IFN-γ.Overdose VitA changed Th1 type induced by BCG inoculation,provocated non-Th1,non-Th2 reaction.Conclusions: BCG unite high dose of vitamin A possible activation lysosomal hydrolase of cell to aggrav pneumonia at acute stage,can provocated non-Th1,non-Th2 reaction and shorten the course of disease.
2009, 34(3): 195-197.
Abstract:
Objective: To observe the inhibitory effects of sanchi powder on the growth of tumor and the influence of immunologic function on sarcoma 180(S180 bearing mice,and to supply experimental data for the research of high efficiency and low toxicity antitumor drugs.Methods: The models of S180 ascitic tumor and solid tumor were established and divided respectively into sodium carboxymethycellulose(SCMC) group,cyclophosph-amide(CTX) group and sanchi powder of low(3 mg/kg),middle(6 mg/kg) and high(12 mg/kg) groups;then the life span and the life extension rate of the mice with ascitic tumor were observed.The weight of the tumor,spleen,and thymus was measured;the antitumor rate,indexes of spleen and thymus of the mice with solid tumor were caculated;the statistical analysis was made among the groups.Results: Compared with the SCMC group,the survival rates of the middle and high dose sanchi powder groups and CTX group increased(P<0.05-P<0.01) and the tumor weight decreased(P<0.05-P<0.01).Compared with the SCMC group and CTX group,the indexes of spleen and thymus of both the middle and high dose sanchi powder groups increased(P<0.05-P<0.01).Conclusions: Sanchi powder has inhibitory effects on the growth of transplanted tumor and may enhance the immunologic function of S180 bearing mice.
Objective: To observe the inhibitory effects of sanchi powder on the growth of tumor and the influence of immunologic function on sarcoma 180(S180 bearing mice,and to supply experimental data for the research of high efficiency and low toxicity antitumor drugs.Methods: The models of S180 ascitic tumor and solid tumor were established and divided respectively into sodium carboxymethycellulose(SCMC) group,cyclophosph-amide(CTX) group and sanchi powder of low(3 mg/kg),middle(6 mg/kg) and high(12 mg/kg) groups;then the life span and the life extension rate of the mice with ascitic tumor were observed.The weight of the tumor,spleen,and thymus was measured;the antitumor rate,indexes of spleen and thymus of the mice with solid tumor were caculated;the statistical analysis was made among the groups.Results: Compared with the SCMC group,the survival rates of the middle and high dose sanchi powder groups and CTX group increased(P<0.05-P<0.01) and the tumor weight decreased(P<0.05-P<0.01).Compared with the SCMC group and CTX group,the indexes of spleen and thymus of both the middle and high dose sanchi powder groups increased(P<0.05-P<0.01).Conclusions: Sanchi powder has inhibitory effects on the growth of transplanted tumor and may enhance the immunologic function of S180 bearing mice.
2009, 34(3): 198-200.
Abstract:
Objective: To explore the expression of survivin and its correlation with Bcl-2 in esophageal cancer.Methods: Forty esophageal cancer's samples,12 samples of normal esophageal tissues adjacent to esophageal cancer and 9 esophagitis samples were collected,and immunohistochemical SP method was used to detect the expression of survivin and Bcl-2 protein.Results: Positive survivin protein expression was detected in 82.5% esophageal cancerous tissues,which was significantly different from that of the esophageal tissues adjacent to esophageal cancer and the esophagitis tissues(P<0.005).Survivin protein expression was detected in esophagitis with atypical hyperplasia,and the expression of survivin was not related to its differentiation degree or the patients' age(P>0.05).The expression of survivin in esophageal cancer was correlated to that of Bcl-2(P=0.030).Conclusions: The expression of survivin in esophageal cancer tissue is up-regulated,but has no association with its pathology type or differentiation degree.survivin protein is correlated to the expression of Bcl-2.The expression of survivin is detected in esophagitis with atypical hyperplasia,which indicates that survivin may participate in the genesis of esophageal cancer and play a role in the progress of esophageal cancer.
Objective: To explore the expression of survivin and its correlation with Bcl-2 in esophageal cancer.Methods: Forty esophageal cancer's samples,12 samples of normal esophageal tissues adjacent to esophageal cancer and 9 esophagitis samples were collected,and immunohistochemical SP method was used to detect the expression of survivin and Bcl-2 protein.Results: Positive survivin protein expression was detected in 82.5% esophageal cancerous tissues,which was significantly different from that of the esophageal tissues adjacent to esophageal cancer and the esophagitis tissues(P<0.005).Survivin protein expression was detected in esophagitis with atypical hyperplasia,and the expression of survivin was not related to its differentiation degree or the patients' age(P>0.05).The expression of survivin in esophageal cancer was correlated to that of Bcl-2(P=0.030).Conclusions: The expression of survivin in esophageal cancer tissue is up-regulated,but has no association with its pathology type or differentiation degree.survivin protein is correlated to the expression of Bcl-2.The expression of survivin is detected in esophagitis with atypical hyperplasia,which indicates that survivin may participate in the genesis of esophageal cancer and play a role in the progress of esophageal cancer.
2009, 34(3): 201-204.
Abstract:
Objective: To observe the effect of noninvasive positive pressure ventilation(NIPPV) on senile severe cardiogenic pulmonary edema(SACPE).Methods: Thirty-eight cases with SACPE were randomly divided into treatment group and control group.Seventeen cases in control group were administered just conventional therapy,while the 21 cases in treatment group received NIPPV in addition to the conventional therapy.The cases' arterial blood gases analysis,heart rates,respiratory rates and chest X-ray appearance were monitored and analyzed.Results: Thirty minutes after the application of NIPPV,the patients' dyspnea was improved markedly,respiratory rates and heart rates decreased,and moist rale disappeared rapidly.The patients in the treatment group improved more rapidly than those in control group.The arterial blood gas analysis showed that the PaO2 rosed more quickly in the treatment group than in the control group.Conclusions: NIPPV is a safe and effective method for treatment of SACPE.
Objective: To observe the effect of noninvasive positive pressure ventilation(NIPPV) on senile severe cardiogenic pulmonary edema(SACPE).Methods: Thirty-eight cases with SACPE were randomly divided into treatment group and control group.Seventeen cases in control group were administered just conventional therapy,while the 21 cases in treatment group received NIPPV in addition to the conventional therapy.The cases' arterial blood gases analysis,heart rates,respiratory rates and chest X-ray appearance were monitored and analyzed.Results: Thirty minutes after the application of NIPPV,the patients' dyspnea was improved markedly,respiratory rates and heart rates decreased,and moist rale disappeared rapidly.The patients in the treatment group improved more rapidly than those in control group.The arterial blood gas analysis showed that the PaO2 rosed more quickly in the treatment group than in the control group.Conclusions: NIPPV is a safe and effective method for treatment of SACPE.
2009, 34(3): 204-205.
Abstract:
Objective: To explore the optimal surgical approach for treatmemt of advanced cardiac cancer.Methods: Twenty-two cases of carcinoma of gastric cardia were operated on by the approach of abdominal cavity and diaphragm.Median abdominal incision was adopted to all the cases.The stomach was dissociated conventionally,the diaphragma dissected from centrum tendineum front oesophagus and the encroached diaphragmu excised.Results: Of the 22 cases,1 case of anastomotic leakage was cured by nonoperative treatment and 1 case of celiac haemorrhage was cured by reoperation.No patients died during the operation;the average hospital stay was 14 days.Conclusions: The surgical approach is adapt to the cases whose carcinoma has encroached diaphragum or inferior oesophagus and the cases whose excision of surerior incisal margin is felt discontented.As the approach has no thoracic incision,the wound and syndrome of heart and lung is decreased.This approach is especially suited to the senior and high risk patients.
Objective: To explore the optimal surgical approach for treatmemt of advanced cardiac cancer.Methods: Twenty-two cases of carcinoma of gastric cardia were operated on by the approach of abdominal cavity and diaphragm.Median abdominal incision was adopted to all the cases.The stomach was dissociated conventionally,the diaphragma dissected from centrum tendineum front oesophagus and the encroached diaphragmu excised.Results: Of the 22 cases,1 case of anastomotic leakage was cured by nonoperative treatment and 1 case of celiac haemorrhage was cured by reoperation.No patients died during the operation;the average hospital stay was 14 days.Conclusions: The surgical approach is adapt to the cases whose carcinoma has encroached diaphragum or inferior oesophagus and the cases whose excision of surerior incisal margin is felt discontented.As the approach has no thoracic incision,the wound and syndrome of heart and lung is decreased.This approach is especially suited to the senior and high risk patients.
2009, 34(3): 206-208.
Abstract:
Objective: To investigate the relationship between fibrinogen-β-845A/G polymorphism and premature coronary heart disease.Methods: Seventy-eight cases of premature coronary heart disease and 81 controls were enrolled in the study.Phenol-trichlormethane was employed to extract DNA,polymerase chain reaction-restriction fragment length polymorphism(PCR-RELP) was used to analyze Fg-β gene polymorphism,and the clinical data were collected.The blood sugar,blood lipids and other biological indicators were tested.Results: There was no significant difference in genotype distribution and alleles of fibrinogen-β-845A/G between patients with premature coronary heart disease and controls(P>0.05),but the difference was significant in total cholesterol,low-density lipoprotein-C,apolipoprotein B and lipoprotein-a between the two groups(P<0.05-P<0.01).Conclusions: The results suggest that fibrinogen-β-845A/G poly-morphism may not be associated with the susceptivity of premature coronary heart disease in China.
Objective: To investigate the relationship between fibrinogen-β-845A/G polymorphism and premature coronary heart disease.Methods: Seventy-eight cases of premature coronary heart disease and 81 controls were enrolled in the study.Phenol-trichlormethane was employed to extract DNA,polymerase chain reaction-restriction fragment length polymorphism(PCR-RELP) was used to analyze Fg-β gene polymorphism,and the clinical data were collected.The blood sugar,blood lipids and other biological indicators were tested.Results: There was no significant difference in genotype distribution and alleles of fibrinogen-β-845A/G between patients with premature coronary heart disease and controls(P>0.05),but the difference was significant in total cholesterol,low-density lipoprotein-C,apolipoprotein B and lipoprotein-a between the two groups(P<0.05-P<0.01).Conclusions: The results suggest that fibrinogen-β-845A/G poly-morphism may not be associated with the susceptivity of premature coronary heart disease in China.
2009, 34(3): 209-211.
Abstract:
Objective: To probe into the relationship between serum homocysteine(Hcy) levels and carotid atherosclerosis in young patients with cerebral infarction.Methods: Forty-four young patients with cerebral infarction were classified into carotid atherosclerosis group and non-carotid atherosclerosis group,and 40 cases with no cerebrovascular dieases acted as controls.The serum Hcy levels were measured by microparticle enzyme immunoassay.Results: The mean serum Hcy level in the patients with cerebral infarction was significantly higher than that in controls(P<0.01),while the mean serum Hcy level in the cerebral infarction group with carotid atherosclerosis was higher than that in non-carotid atherosclerosis group(P<0.05) and the control group(P<0.01).Conclusions: Serum Hcy levels are closely related with carotid atherosclerosis and is an important risk factor for cerebral infarction in young adults.
Objective: To probe into the relationship between serum homocysteine(Hcy) levels and carotid atherosclerosis in young patients with cerebral infarction.Methods: Forty-four young patients with cerebral infarction were classified into carotid atherosclerosis group and non-carotid atherosclerosis group,and 40 cases with no cerebrovascular dieases acted as controls.The serum Hcy levels were measured by microparticle enzyme immunoassay.Results: The mean serum Hcy level in the patients with cerebral infarction was significantly higher than that in controls(P<0.01),while the mean serum Hcy level in the cerebral infarction group with carotid atherosclerosis was higher than that in non-carotid atherosclerosis group(P<0.05) and the control group(P<0.01).Conclusions: Serum Hcy levels are closely related with carotid atherosclerosis and is an important risk factor for cerebral infarction in young adults.
2009, 34(3): 211-213.
Abstract:
Objective: To observe the clinic effect of microendoscropic discectomy(MED) operation on lumbar disc herniation(LDH) complicated with lumbar spinal stenosis.Methods: Thirty-two cases of lumbar disc herniation complicated with lumbar spinal stenosis or lateral recess stenosis underwent posterior microendoscopic discectomy,and the clinical effects were reviewed and analyzed.Results: The follow-up continued from 4 months to 38 months.The evaluation with Nakai scale revealed excellent in 25 cases,good in 4 cases,fair in 2 cases and poor in 1 case.The rate of good results was 90.6%(29/32).According to the clinical effect scale,the results by spinal micro-endoscopy therapy were excellent in 25 cases,good in 3 cases,fair in 3 cases and poor in 1 case,with an effective rate of 87.5%(28/32).Conclusions: Posterior microendoscropic discectomy shows minimal invasion,quick recovery and good clinic effect,it is safe and effective in treatment of lumbar disc herniation complicated with lumbar spinal stenosis.
Objective: To observe the clinic effect of microendoscropic discectomy(MED) operation on lumbar disc herniation(LDH) complicated with lumbar spinal stenosis.Methods: Thirty-two cases of lumbar disc herniation complicated with lumbar spinal stenosis or lateral recess stenosis underwent posterior microendoscopic discectomy,and the clinical effects were reviewed and analyzed.Results: The follow-up continued from 4 months to 38 months.The evaluation with Nakai scale revealed excellent in 25 cases,good in 4 cases,fair in 2 cases and poor in 1 case.The rate of good results was 90.6%(29/32).According to the clinical effect scale,the results by spinal micro-endoscopy therapy were excellent in 25 cases,good in 3 cases,fair in 3 cases and poor in 1 case,with an effective rate of 87.5%(28/32).Conclusions: Posterior microendoscropic discectomy shows minimal invasion,quick recovery and good clinic effect,it is safe and effective in treatment of lumbar disc herniation complicated with lumbar spinal stenosis.
2009, 34(3): 214-215.
Abstract:
Objective: To investigate the role of cognitive behavioral therapy(CBT) in the restoration process in patients with post-stroke depression(PSD).Methods: Forty patients with PSD were randomly divided into observation group and control group.The control group were administered only Amitriptyline for oral use,while the observation group received CBT in addition to Amitriptyline.All the patients were evaluated by Hamilton depression scale(HAMD) every two months from the first month to the eleventh month after the attack.Results: After using CBT for six months,the HAMD in the observation group was significantly lowered compared with that in the control group(P<0.05).Conclusions: CBT has an obvious effect on patients with PSD in the restoration process.
Objective: To investigate the role of cognitive behavioral therapy(CBT) in the restoration process in patients with post-stroke depression(PSD).Methods: Forty patients with PSD were randomly divided into observation group and control group.The control group were administered only Amitriptyline for oral use,while the observation group received CBT in addition to Amitriptyline.All the patients were evaluated by Hamilton depression scale(HAMD) every two months from the first month to the eleventh month after the attack.Results: After using CBT for six months,the HAMD in the observation group was significantly lowered compared with that in the control group(P<0.05).Conclusions: CBT has an obvious effect on patients with PSD in the restoration process.
2009, 34(3): 216-218.
Abstract:
Objective: To evaluate the clinical efficacy of superficial iliac circumflex artery island flaps in repairing soft tissue defects in hand.Methods: Superficial iliac circumflex artery island flaps was used to repair nine cases of wound hands.The split-thickness skin grafting was applied to recover the donor sites of the defects in 2 cases and the other donor sites were sutured directly.The flaps at the distance of one third were thinned in 2 cases.Results: The pedicles were severed for 14 to 30 days.All the 9 cases were repaired at one stage.The flaps were all survived and achieved satisfactory results in 3 months to 1 year's follow-up,excepting that the sense of pain,temperature and touch were a little dull.Conclusions: The procedure of repairing soft tissue defecs in hand with superficial iliac circumflex artery island flap is easy and practical,with the adavantages of stable blood vessels,high survival rate and few complications.This method is worth popularizing.
Objective: To evaluate the clinical efficacy of superficial iliac circumflex artery island flaps in repairing soft tissue defects in hand.Methods: Superficial iliac circumflex artery island flaps was used to repair nine cases of wound hands.The split-thickness skin grafting was applied to recover the donor sites of the defects in 2 cases and the other donor sites were sutured directly.The flaps at the distance of one third were thinned in 2 cases.Results: The pedicles were severed for 14 to 30 days.All the 9 cases were repaired at one stage.The flaps were all survived and achieved satisfactory results in 3 months to 1 year's follow-up,excepting that the sense of pain,temperature and touch were a little dull.Conclusions: The procedure of repairing soft tissue defecs in hand with superficial iliac circumflex artery island flap is easy and practical,with the adavantages of stable blood vessels,high survival rate and few complications.This method is worth popularizing.
2009, 34(3): 218-219.
Abstract:
Objective: To study the value of neonatal behavioral neurological assessment(NBNA) in the prognosis of high-risk neonates.Methods: NBNA was carrried out in 54 high-risk neonates 7 to 28 days after birth.The neonates with marks below or equal to 35 were included into the abnormal group,while those with marks above 35 were in the normal group.All the children were followed up to 3 to 6 years of age and China Child Developmental Centre Checklist was used to determine their intellectual and physical development.Results: The rate of poor prognosis was much higher in the abnormal group than in the normal group during 3 to 6 years after birth(P<0.005).Conclusions: The dynamic marking of NBNA is of great value in forecasting the prognosis of high-risk neonates in perinatal period,which may provide basis for early intervention.
Objective: To study the value of neonatal behavioral neurological assessment(NBNA) in the prognosis of high-risk neonates.Methods: NBNA was carrried out in 54 high-risk neonates 7 to 28 days after birth.The neonates with marks below or equal to 35 were included into the abnormal group,while those with marks above 35 were in the normal group.All the children were followed up to 3 to 6 years of age and China Child Developmental Centre Checklist was used to determine their intellectual and physical development.Results: The rate of poor prognosis was much higher in the abnormal group than in the normal group during 3 to 6 years after birth(P<0.005).Conclusions: The dynamic marking of NBNA is of great value in forecasting the prognosis of high-risk neonates in perinatal period,which may provide basis for early intervention.
2009, 34(3): 220-222.
Abstract:
Objective: To investigate the expression of p16 in the carcinogenesis of cervical cancer.Methods: The expression of p16 in the tissue of 46 cases of cervical intraepithelial neoplasia and 41 cases of uterine cervix cancer were examined by using immunohis-tochemical method SABC Ten cases of normal tissues acted as control.Results: Expression of p16 in the tissue of cervical intraepithelial neoplasia was higher than that in the control(P>0.05),and that low in the tissue of cervical cancer was lower than that in the control(P<0.05).In the cervical cancer group,the expression of p16 was correlated to the histological grade,clinical stage and lympho node metastasis(P<0.05).Conclusions: The results suggest that p16 may play an important role in the carcinogensis of cervical cancers.The low expression of p16 is correlated to the malicious biological behaviors of cervical cancers.p16 may be used as an important maker to evaluate the malignancy degree and the prognosis of cervical cancer.
Objective: To investigate the expression of p16 in the carcinogenesis of cervical cancer.Methods: The expression of p16 in the tissue of 46 cases of cervical intraepithelial neoplasia and 41 cases of uterine cervix cancer were examined by using immunohis-tochemical method SABC Ten cases of normal tissues acted as control.Results: Expression of p16 in the tissue of cervical intraepithelial neoplasia was higher than that in the control(P>0.05),and that low in the tissue of cervical cancer was lower than that in the control(P<0.05).In the cervical cancer group,the expression of p16 was correlated to the histological grade,clinical stage and lympho node metastasis(P<0.05).Conclusions: The results suggest that p16 may play an important role in the carcinogensis of cervical cancers.The low expression of p16 is correlated to the malicious biological behaviors of cervical cancers.p16 may be used as an important maker to evaluate the malignancy degree and the prognosis of cervical cancer.
2009, 34(3): 223-225.
Abstract:
Objective: To summarize the experience in treatment of infective endocarditis(IE) by surgical operation and to evaluate the curative effect.Methods: Among 34 patients with IE,mitral valve replacement was performed in 9 cases,arotic valve replacement in 12,double valve replacement in 6,tricuspid valve replacement in 1,tricuspid valve reconstruction in 1,ventricular septal defect neoplasty in 1,atrial septal defect neoplasty in 1 and VSD with ruptured aortic sinus aneurysm in 1.And 3 of the cases received emergency operation.Results: Thirty-two of the cases were cured.The follow-up lasted from one to five years,with no record of recurrence or death.The cause of 2 deaths after operation was multiple organ system failure.Conclusions: Precise diagnosis of IE depends on hemoculture and ultrasound cardiogram;the key to sucessful operations are proper management during perioperative period and purposeful procedure in operation.
Objective: To summarize the experience in treatment of infective endocarditis(IE) by surgical operation and to evaluate the curative effect.Methods: Among 34 patients with IE,mitral valve replacement was performed in 9 cases,arotic valve replacement in 12,double valve replacement in 6,tricuspid valve replacement in 1,tricuspid valve reconstruction in 1,ventricular septal defect neoplasty in 1,atrial septal defect neoplasty in 1 and VSD with ruptured aortic sinus aneurysm in 1.And 3 of the cases received emergency operation.Results: Thirty-two of the cases were cured.The follow-up lasted from one to five years,with no record of recurrence or death.The cause of 2 deaths after operation was multiple organ system failure.Conclusions: Precise diagnosis of IE depends on hemoculture and ultrasound cardiogram;the key to sucessful operations are proper management during perioperative period and purposeful procedure in operation.
2009, 34(3): 225-227.
Abstract:
Objective: To explore the clinical features and cytological changes of cerebrospinal fluid(CSF) in meningeal carcinomatasis.Methods: The clinical features and cytological changes of CSF of two meningeal carcinomatasis patients were analyzed,and the literature was reviewed.Results: Of the two cases of meningeal carcinomatasis,one was due to acute change of chronic myelocytic leukemia and the other had received surgery for breast cancer.The main clinical manifestations were headache and vomiting.No spinal nervous lesion or signs of meningeal irritation were observed.Abnormal level of intracranial pressure,increased cell count and protein level were found in CSF examination.Cancer cells were found in cytological examination Conclusions: The cytological examination of CSF is essential for the diagnosis of meningeal carcinomatasis.
Objective: To explore the clinical features and cytological changes of cerebrospinal fluid(CSF) in meningeal carcinomatasis.Methods: The clinical features and cytological changes of CSF of two meningeal carcinomatasis patients were analyzed,and the literature was reviewed.Results: Of the two cases of meningeal carcinomatasis,one was due to acute change of chronic myelocytic leukemia and the other had received surgery for breast cancer.The main clinical manifestations were headache and vomiting.No spinal nervous lesion or signs of meningeal irritation were observed.Abnormal level of intracranial pressure,increased cell count and protein level were found in CSF examination.Cancer cells were found in cytological examination Conclusions: The cytological examination of CSF is essential for the diagnosis of meningeal carcinomatasis.
2009, 34(3): 228-229.
Abstract:
Objective: To investigate the clinical trait and the treatment of diabetic ketoacidosis inpatients with renal function impairment.Methods: The indexes such as inducement,blood sugar,blood ketone bodies,pH,white blood cell,serum creatinine,blood urea nitrogen were analyzed retrospectively.Results: Renal function of 13 cases regained health completely.Renal function of 5 cases regained health partly.One case gave up the treatment.No died case.Conclusions: Renal function impairment of diabetic ketoacidosis inpatients mostly was transitory,which was connected with dehydration,acidosis,micro-cirulation disturbance.Partly renal function impairment cases which could not regain health completely were diabetic nephropathy.
Objective: To investigate the clinical trait and the treatment of diabetic ketoacidosis inpatients with renal function impairment.Methods: The indexes such as inducement,blood sugar,blood ketone bodies,pH,white blood cell,serum creatinine,blood urea nitrogen were analyzed retrospectively.Results: Renal function of 13 cases regained health completely.Renal function of 5 cases regained health partly.One case gave up the treatment.No died case.Conclusions: Renal function impairment of diabetic ketoacidosis inpatients mostly was transitory,which was connected with dehydration,acidosis,micro-cirulation disturbance.Partly renal function impairment cases which could not regain health completely were diabetic nephropathy.
2009, 34(3): 229-231.
Abstract:
Objective: To study the expression of X-linked inhibitor of apoptosis protein(XIAP) in bladder transitional cell carcinoma(BTCC) and to clarify the relationship between the pathological grades and clinical stages of BTCC.Methods: The epithelium speciment of 62 cases of BTCC confirmed by pathological method were collected and the expression of XIAP was detected by using immunohistochemical assay(SP methods).Results: The positive expression rate of XIAP in BTCC was 67.7%,which was significantly higher than that of normal bladder tissues(23.1%)(P<0.005).The positive expression rates in grade Ⅰ,Ⅱ and Ⅲ were 75.0%,57.9% and 66.7%,respectively;The positive expression rates in stage Ta-T1 and T2-T4 were 63.9% and 73.1%,respectively.Conclusions: It is suggested that high expression of XIAP may play an important role in the mechanisms of tumorignesis and development of BTCC.
Objective: To study the expression of X-linked inhibitor of apoptosis protein(XIAP) in bladder transitional cell carcinoma(BTCC) and to clarify the relationship between the pathological grades and clinical stages of BTCC.Methods: The epithelium speciment of 62 cases of BTCC confirmed by pathological method were collected and the expression of XIAP was detected by using immunohistochemical assay(SP methods).Results: The positive expression rate of XIAP in BTCC was 67.7%,which was significantly higher than that of normal bladder tissues(23.1%)(P<0.005).The positive expression rates in grade Ⅰ,Ⅱ and Ⅲ were 75.0%,57.9% and 66.7%,respectively;The positive expression rates in stage Ta-T1 and T2-T4 were 63.9% and 73.1%,respectively.Conclusions: It is suggested that high expression of XIAP may play an important role in the mechanisms of tumorignesis and development of BTCC.
2009, 34(3): 232-233.
Abstract:
Objective: To improve the level of early diagnoses of the pituitary adenoma,through studying vision function damage and the reason why misdiagnoses taken place to the patients suffered from pituitary adenoma.Methods: Clinical analysis was done on 37 patients who have been confirmed suffering from pituitary adenoma through pathologic diagnosis.Results: Through clinical observation to the 37 patients,there are 35 patients whose vision function were damaged and there are 34 patients whose vision field were damaged,15 patients came to see ophthalmologists because they took the reduce of vision acuity as the first symptom,5 patients were misdiagnosed.Conclusions: During clinical diagnoses,the ophthalmologists should do synthetical analysis to the patients who have abnormal vision acuity and vision field,in order to avoid or reduce the misdiagnoses to the pituitary adenoma.
Objective: To improve the level of early diagnoses of the pituitary adenoma,through studying vision function damage and the reason why misdiagnoses taken place to the patients suffered from pituitary adenoma.Methods: Clinical analysis was done on 37 patients who have been confirmed suffering from pituitary adenoma through pathologic diagnosis.Results: Through clinical observation to the 37 patients,there are 35 patients whose vision function were damaged and there are 34 patients whose vision field were damaged,15 patients came to see ophthalmologists because they took the reduce of vision acuity as the first symptom,5 patients were misdiagnosed.Conclusions: During clinical diagnoses,the ophthalmologists should do synthetical analysis to the patients who have abnormal vision acuity and vision field,in order to avoid or reduce the misdiagnoses to the pituitary adenoma.
2009, 34(3): 234-236.
Abstract:
Objective: To evaluate the efficacy and safety of endovascular stent-graft exclusion in treatment of thoracic and abdominal aneurysms.Methods: Seven patients with thoracic and abdominal aneurysms underwent endovascular stent-graft exclusion under general anaesthesia or basic anaesthesia.Home-made stent-grafts were used in 6 cases with thoracic aortic disease and TALENT bifurcated stent-graft was used in 1 case.Results: The pseudocoele and aneurysm in 6 cases were effectively isolated and the ulcer in 1 case healed.CT angiography showed that no endoleak or stent-graft disposition occurred and all the patients were capable of normal physical work after operation.Conclusions: Endovascular stent-graft exclusion can be used for treatment of thoracic and abdominal aneurysms.It is a promising microoperation therapy.
Objective: To evaluate the efficacy and safety of endovascular stent-graft exclusion in treatment of thoracic and abdominal aneurysms.Methods: Seven patients with thoracic and abdominal aneurysms underwent endovascular stent-graft exclusion under general anaesthesia or basic anaesthesia.Home-made stent-grafts were used in 6 cases with thoracic aortic disease and TALENT bifurcated stent-graft was used in 1 case.Results: The pseudocoele and aneurysm in 6 cases were effectively isolated and the ulcer in 1 case healed.CT angiography showed that no endoleak or stent-graft disposition occurred and all the patients were capable of normal physical work after operation.Conclusions: Endovascular stent-graft exclusion can be used for treatment of thoracic and abdominal aneurysms.It is a promising microoperation therapy.
2009, 34(3): 243-245.
Abstract:
Objective: To assess the clinical value of selective transcatheter arterial embolization(TAE) in nasopharyngeal angiofibroma before operation.Methods: Six cases of nasopharyngeal angiofibroma were embolized with silk particles and gelatin sponge under digital subtraction angiography(DSA),and surgical resection was performed under endoscope 2 days later.Results: All the cases were successfully embolized at one time.The mean amount of haemorrhage during operation was 50-300 ml.Computed Tomography showed that the nasopharyngeal angiofibroma was completely removed in all the cases.Conclusions: Selective transcatheter arterial embolization before resection of nasopharyngeal angiofibroma can reduce the mean amount of haemorrhage during operation,which promises an extensive prospect in wider application.
Objective: To assess the clinical value of selective transcatheter arterial embolization(TAE) in nasopharyngeal angiofibroma before operation.Methods: Six cases of nasopharyngeal angiofibroma were embolized with silk particles and gelatin sponge under digital subtraction angiography(DSA),and surgical resection was performed under endoscope 2 days later.Results: All the cases were successfully embolized at one time.The mean amount of haemorrhage during operation was 50-300 ml.Computed Tomography showed that the nasopharyngeal angiofibroma was completely removed in all the cases.Conclusions: Selective transcatheter arterial embolization before resection of nasopharyngeal angiofibroma can reduce the mean amount of haemorrhage during operation,which promises an extensive prospect in wider application.
2009, 34(3): 246-247.
Abstract:
Objective: To study the effects of interventional therapy with the help of general ultrasonic detectors for treatment of lesions containing liquid.Methods: Retrospective research was applied to 165 cases of lesions containing liquid which had been diagnosed and treated with interventional therapy under general ultrasonic detectors.Results: All the 165 cases were cured,including some severe cases who achieved satisfactory results after 3 times of treatments at the most.Conclusions: Interventional therapy under the guidiance of general ultrasonic detectors has affirmative curative effects on lesions containing liquid.
Objective: To study the effects of interventional therapy with the help of general ultrasonic detectors for treatment of lesions containing liquid.Methods: Retrospective research was applied to 165 cases of lesions containing liquid which had been diagnosed and treated with interventional therapy under general ultrasonic detectors.Results: All the 165 cases were cured,including some severe cases who achieved satisfactory results after 3 times of treatments at the most.Conclusions: Interventional therapy under the guidiance of general ultrasonic detectors has affirmative curative effects on lesions containing liquid.
2009, 34(3): 248-250.
Abstract:
Objective: To explore the diagnostic value of high frequency ultrasound in testicular microlithiasis(TM) and its clinical significance.Methods: The sonography and the stream parameters of the testicular artery of 30 cases of testicular microlithiasis were retrospectively analyzed and 30 healthy persons acted as controls.Results: Of the 30 cases of testicular microlithiasis,22 cases were involved with both of the testes and 8 cases with only one testis.Sonographic features showed rare or diffuse high-echo dots in the testicular parenchyma with a diameter of 1-2 mm,devoid of shadow.There was no significant difference in blood stream parameters of testicular artery between TM group and the control group(P>0.05).Conclusions: High frequency ultrasound can accurately diagnose TM.It is the first-choice of method.
Objective: To explore the diagnostic value of high frequency ultrasound in testicular microlithiasis(TM) and its clinical significance.Methods: The sonography and the stream parameters of the testicular artery of 30 cases of testicular microlithiasis were retrospectively analyzed and 30 healthy persons acted as controls.Results: Of the 30 cases of testicular microlithiasis,22 cases were involved with both of the testes and 8 cases with only one testis.Sonographic features showed rare or diffuse high-echo dots in the testicular parenchyma with a diameter of 1-2 mm,devoid of shadow.There was no significant difference in blood stream parameters of testicular artery between TM group and the control group(P>0.05).Conclusions: High frequency ultrasound can accurately diagnose TM.It is the first-choice of method.
2009, 34(3): 250-251.
Abstract:
Objective: To establish a high performance liquid chromatography(HPLC) method for the determination of caffeic acid in compound decoction of Yinchenhao and Huanglianjiedu(CDYH).Methods: HPLC was used with Hypersil BDS C18(5 μm,250 mm×4.6 mm) and the methanolu potassium dihydrogen phosphate(pH=3.7) asmobile phase in gradientmode.The UV detective wave length was at 323 nm.Results: There was a good linear relationship within the concentration range of 8-40 μg/ml in caffeic acid and ferulic acid in CDYH(r=0.999 7,P<0.01).The average recoveries of caffeic acid and ferulic acid were 99.29%,RSD=0.81% and 99.38%,RSD=0.65%.Conclusions: This method is simple,rapid and accurative.
Objective: To establish a high performance liquid chromatography(HPLC) method for the determination of caffeic acid in compound decoction of Yinchenhao and Huanglianjiedu(CDYH).Methods: HPLC was used with Hypersil BDS C18(5 μm,250 mm×4.6 mm) and the methanolu potassium dihydrogen phosphate(pH=3.7) asmobile phase in gradientmode.The UV detective wave length was at 323 nm.Results: There was a good linear relationship within the concentration range of 8-40 μg/ml in caffeic acid and ferulic acid in CDYH(r=0.999 7,P<0.01).The average recoveries of caffeic acid and ferulic acid were 99.29%,RSD=0.81% and 99.38%,RSD=0.65%.Conclusions: This method is simple,rapid and accurative.
2009, 34(3): 252-254.
Abstract:
Objective: To observe the curative effect of hyperbaric oxygen in treatment of craniocerebral trauma of children and to sum up the experience in the nursing care.Methods: One hundred and sixty cases(82 children in observation group and 78 in control group) were analyzed retrospectively.The observation group were applied hyperbaric oxygenation in addition to the conventional therapy;at the same time,holistic nursing care,such as mental nursing,was performed,and knowledge about the hyperbaric oxygenation was provided to the parents to get their coordination.The control group were administered only the routine therapy.Results: The overall response rate of the observation group was obviously higher than that of the control group(P<0.005).Conclusions: Hyperbaric oxygenation is effective for treatment of craniocerebral trauma in children.Meanwhile,appropriate nursing care is essential.
Objective: To observe the curative effect of hyperbaric oxygen in treatment of craniocerebral trauma of children and to sum up the experience in the nursing care.Methods: One hundred and sixty cases(82 children in observation group and 78 in control group) were analyzed retrospectively.The observation group were applied hyperbaric oxygenation in addition to the conventional therapy;at the same time,holistic nursing care,such as mental nursing,was performed,and knowledge about the hyperbaric oxygenation was provided to the parents to get their coordination.The control group were administered only the routine therapy.Results: The overall response rate of the observation group was obviously higher than that of the control group(P<0.005).Conclusions: Hyperbaric oxygenation is effective for treatment of craniocerebral trauma in children.Meanwhile,appropriate nursing care is essential.
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