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2021 Vol. 46, No. 12
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2021, 46(12): 1645-1648, 1653.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.001
Abstract:
ObjectiveTo investigate the mechanism of glycolysis inhibitor WZB117-induced apoptosis in human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptotic pathway. MethodsGastric cancer cell line MGC-803 was used in this study, and exponential growth cells were used in the experiment.According to the experimental requirements, the cultured cells were divided into control group(normally cultured cells) and WZB117 group(cells treated with 20 μg/mL glucose transporter inhibitor).The proliferation of cells was detected by MTS kit.Cell viability was measured by CCK-8 and apoptosis was analyzed by TUNEL.Mitochondrial function was detected by ATP content.The expression of Bcl-2, Bax, caspase-3, Cyt-c and glycolysis-related enzymes cells in hexokinase(HK) and cells in phosphofructokinase(PFK) were analyzed by Western blotting. ResultsThe proliferation of WZB117 group was lower than that in control group at 24 h and 48 hours(P < 0.01).The apoptosis rate in WZB117 group was higher than that in control group(P < 0.01), and the cell viability in WZB117 group was lower than that in control group(P < 0.01).The ATP content in WZB117 group was lower than that in control group at 12 h and 24 h(P < 0.01).The expression of Bcl-2 protein in WZB117 group was lower than that in control group(P < 0.01), and the expression of Bax, caspase-3 and Cyt-c protein in WZB117 group was higher than that in control group(P < 0.01).The expression of HK and PFK in WZB117 group was lower than that in control group(P < 0.01), indicating that WZB117 could inhibit the expression of enzymes related to glycolysis. ConclusionsWZB117 induces apoptosis of gastric cancer cell line MGC-803 by inhibiting glycolysis pathways and reducing mitochondrial ATP production.
2021, 46(12): 1649-1653.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.002
Abstract:
ObjectiveTo explore the molecular mechanism of circ_0000515 regulating the proliferation and apoptosis of colon cancer cells. MethodsA total of 41 cases of colon cancer patients with cancer tissue and adjacent tissue samples were selected.Real-time fluorescent quantitative PCR was used to detect the expression of circ_0000515 and miR-1258.Colon cancer cells SW620 were divided into si-circ_0000515 group, si-NC group, miR-1258 group, miR-NC group, si-circ_0000515+anti-miR-NC group, si-circ_0000515+anti-miR-1258 group.Tetramethylazolium salt colorimetric assay(MTT) was used to detect the cell viability.Flow cytometry was used to detect apoptosis.The targeting relationship of circ_0000515 and miR-1258 was detected by dual-luciferase reporter assay. ResultsCompared with the adjacent tissues, the expression of circ_0000515 in colon cancer tissues was increased, and the expression of miR-1258 was decreased(P < 0.01).After the inhibition of circ_0000515 expression or overexpression of miR-1258, the viability of SW620 cells was decreased, the apoptosis rate was increased(P < 0.01).Circ_0000515 targeted and regulated the expression of miR-1258.Interference with miR-1258 expression reversed the effect of inhibiting circ_0000515 expression on the proliferation and apoptosis of colon cancer SW620 cells. ConclusionsInhibiting the expression of circ_0000515 by up-regulation of miR-1258 can inhibit the proliferation and promote the apoptosis in colon cancer cells.
2021, 46(12): 1654-1659.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.003
Abstract:
ObjectiveTo investigate the expression levels of microRNA(miR)-17-5p and phosphotase and tensin homolog(PTEN) in diffuse large B-cell lymphoma(DLBCL), and their clinical significance. MethodsThe expression levels of miR-17-5p and PTEN in 82 cases of DLBCL carcinoma tissues and 30 cases of lymph node reactive hyperplasia tissues(control group) were detected using real-time quantitative PCR.The differences of the expression levels of miR-17-5p and PTEN between groups, and their relationship with clinicopathological features were analyzed.According to miR-17-5p sequence, the miR-17-5p inhibitors and inhibitor controls were synthesized, and transfected into the DLBCL cell lines.The MTT cell proliferation assay and Transwell chamber assay were used to observe the effects on the proliferation and invasion ability of tumor cells.The Kaplan-Meier survival analysis was used to analyze the differences between the different expression levels of miR-17-5p and PTEN and prognosis of patients. ResultsThe relative expression level of miR-17-5p in tumor tissues was higher than that in normal tissues(P < 0.01).The relative expression level of PTEN in tumor tissue was lower than that in normal tissue(P < 0.01).The results of Pearson linear correlation analysis showed that the expression level of miR-17-5p was negatively correlated with the PTEN expression in tumor tissues(r=-0.612, P < 0.01).The expression level of miR-17-5p in patients with primary tumor site locating intra-ganglia, lactic dehydrogenase(LDH) ≤ 245 U/L and clinical stagesⅠ-Ⅱ were higher than that in patients with primary tumor site locating extra ganglia, LDH>245 U/L and clinical stage Ⅲ-Ⅳ, and the expression level of PTEN in patients with primary tumor site locating intra-ganglia, LDH ≤ 245 U/L and clinical stagesⅠ-Ⅱ was lower than that in extra ganglia, LDH>245 and linical stage Ⅲ-Ⅳ(P < 0.05 to P < 0.01).Compared with the transfection inhibitor control, the expression levels of miR-17-5p and PTEN in SU-DHL 8 cells transfected with miR-17-5p inhibitor were lower and higher, respectively(P < 0.01).Compared with the transfection inhibitor control, the proliferation and invasion ability of DLBCL cells transfected with miR-17-5p inhibitor after 96 h was significantly attenuated(P < 0.05).In Transwell chamber assay, the number of transmembrane cells transfected with miR-17-5p inhibitor was significantly reduced compared with the transfection inhibitor control group(P < 0.05).The 3-year overall survival rate of patients with high miR-17-5p was lower than that of patients with low miR-17-5p(P < 0.05), and that of patients with low PTEN expression was lower than that of patients with high PTEN expression(P < 0.05). ConclusionsThe expression level of miR-17-5p increases, while the expression level of PTEN decreases in patients with DLBCL.Both of them are involved in the occurrence and development of DLBCL, and may become a new tumor marker for DLBCL diagnosis and treatment.
2021, 46(12): 1659-1663.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.004
Abstract:
ObjectiveTo explore the intervention effects of tanshinone extract on Alzheimer's disease model rats and its mechanism. MethodsForty healthy male SD rats were randomly divided into the normal group, model group, low-dose group and high-dose group(10 rats in each group).The low-dose group and high-dose group were given 10 g/L and 30 g/L tanshinone extract by intragastric administration, respectively.The normal group and model group were given 0.9% sodium chloride solution by intragastric administration.The learning and memory ability in each group were tested.The levels of interleukin4(IL-4), high-sensitivity C-reactive protein(hs-CRP) and tumor necrosis factor-α(TNF-α) were detected using enzyme-linked immunosorbent assay, the apoptosis of hippocampal cells in each group was detected using flow cytometry, and the protein expression levels of Bcl-2, Bax and caspase-3 were detected using Western blotting. ResultsThe latency of escape in model group was longer than that in normal group(P < 0.01), and the number of crossing platform in model group was lower than that in normal group(P < 0.01).The escape latency in high-dose group was lower than that in model group and low-dose group(P < 0.01), and the number of crossing platform in high-dose group was more than that in model group and low-dose group(P < 0.01 and P < 0.05).The order of hs-CRP, TNF-α and IL-4 levels from high to low was as follows: model group, high-dose group, low-dose group and normal group(P < 0.01).The order of apoptosis rate of hippocampal cells at different time points was as follows: model group, low-dose group, high-dose group and normal group(P < 0.01).The order of Bcl-2 expression in the four groups from high to low was the normal group, high-dose group, low-dose group and model group(P < 0.01).The expression levels of Bax and caspase-3 were in descending order among the four groups: model group, low-dose group, high-dose group and normal group(P < 0.01). ConclusionsThe intervention of tanshinone extract on Alzheimer's disease model rats can improve the learning and memory ability, alleviate the inflammatory reaction of brain tissue, regulate the relative expression of apoptosis-related proteins, Bcl-2, Bax and caspase-3, and inhibit the apoptosis of hippocampal cells, which has certain neuronal protection effect in Alzheimer's disease rats.
2021, 46(12): 1664-1667.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.005
Abstract:
ObjectiveTo explore the effect of the extract of Gleditsia sinensis thorns on the proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cells. MethodsNasopharyngeal carcinoma CNE-2 cells were cultured in vitro.The CNE-2 cells with different concentrations of the extract of Gleditsia sinensis thorns extract, Complete cell culture medium was used as the negative control group.The cell proliferation was detected by MTT assay.Treating nasopharyngeal carcinoma CNE-2 cells with different concentrations of the extract of Gleditsia sinensis thorns, Apoptosis of the cells was detected by flow cytometry. ResultsThe cell proliferation inhibition rates of the higher concentration groups(3.2, 6.4, 12.8, 25.6 mg/mL) were (89.61±1.88)%, (96.99±0.66)%, (93.88±1.24)%, (84.15±0.86)%, respectively, the apoptosis rate of each concentration group was significantly higher than the negative control group's(P < 0.05).After treating nasopharyngeal carcinoma CNE-2 cells with 2, 2.5, 3 mg/mL the extract of Gleditsia sinensis thorns, the apoptosis rate were(15.61±1.21)%, (22.08±1.70)%, (37.62±2.07)%, respectively, the apoptosis rate of each concentration group was significantly higher than the negative control group's(P < 0.01). ConclusionsThe extract of Gleditsia sinensis thorns can inhibit the proliferation and induce the apoptosis of nasopharyngeal carcinoma CNE-2 cells.
2021, 46(12): 1668-1672.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.006
Abstract:
ObjectiveTo explore the effects of miR-503-5p on the proliferation, migration, apoptosis and adhesion of human umbilical vein endothelial cells(HUVECs) induced by interleukin1β(IL-1β), and its molecular mechanism. MethodsThe HUVECs were divided into the NC group, IL-1β group, IL-1β+anti-miR-con group and IL-1β+anti-miR-503-5p group.The proliferation, adhesion, apoptosis and migration ability of cells were detected using MTT assay, adhesion assay, flow cytometry and Transwell assay, respectively.The expression levels of intercellular adhesion molecule 1(ICAM-1) and vascular cell adhesion molecule 1(VCAM-1) were detected using Western blotting. ResultsCompared with the NC group, the cell viability and migrated cell numbers of HUVECs significantly reduced, and the cell adhesion numbers, apoptosis rate, and ICAM-1 and VCAM-1 protein expression levels significantly increased in the IL-1β group(P < 0.01).Compared with the IL-1β+anti-miR-con group, the cell viability and migrated cell numbers of HUVECs significantly increased, and the cell adhesion numbers, apoptosis rate, and ICAM-1 and VCAM-1 protein expression levels significantly decreased in the IL-1β+anti-miR-503-5p group(P < 0.01). ConclusionsInhibiting the miR-503-5p expression can promote the vascular endothelial cell proliferation and migration, and inhibit vascular adhesion and apoptosis, which may be related to the inhibition of ICAM-1 and VCAM-1 expression.
2021, 46(12): 1673-1676.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.007
Abstract:
ObjectiveTo analyze the relationship between the early pain, changes of psychological factors and risk of post-herpetic neuralgia(PHN). MethodsThree hundred and seventy-seven patients with shingles were divided into the PHN group and control group according to whether or not the PHN was merged.The admission time, site of herpes, pain score, anxiety and depression scores were compared between the two groups.The indicators affecting PHN of patients were analyzed using the multivariate logistic regression analysis, and the ROC curve was used to compare the diagnostic value of related factors affecting PHN. ResultsThe age, proportion of diabetes mellitus, autoimmune disease to malignant tumor, visit time, cumulative area, visual analogue scale(VAS) score, dermatology life quality index(DLQI) score, hospital anxiety and depression scale(HADS) score, combined A/G ratio and CD4+/CD8+ abnormality ratio in PHN group were higher than those in control group(P < 0.05 to P < 0.01).There was no statistical significance in gender, smoking history, drinking history, site of disease and type of herpes between the two groups(P>0.05).The results of multivariate logistic analysis showed that the advanced age, long visit time, large rash area, high VAS, DLQI and HADS scores were the independent risk factors of the development of PHN(P < 0.05 to P < 0.01), while the absence of diabetes mellitus was a protective factor of the development of PHN(P < 0.05).The results of ROC curve showed that the diagnostic efficacy of VAS and HADS scores in predicting PHN was significantly higher than that of age, visit time, DLQI score, diabetes mellitus and rash area alone(P < 0.05 to P < 0.01). ConclusionsThe severe early pain, anxiety, depression and other negative psychological factors in patients with herpes zoster are the independent risk factors of the development of PHN, and the VAS combined with HADS score can effectively predict the PHN.
2021, 46(12): 1677-1680.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.008
Abstract:
ObjectiveTo investigate the value of plasma D-dimer in the diagnosis of venous thromboembolism caused by bone injury in patients with bone trauma. MethodsSeventy patients with bone injury were selected.According to the results of color Doppler ultrasonography, 20 patients with and 50 patients without venous thromboembolism were divided into the thrombosis group and bone injury group, respectively.Twenty healthy people at the same time were selected as the control group.The preoperative and postoperative D-dimer levels of all patients were detected, and analyzed using one-way ANOVA.The positive rate of D-dimer in patients with different fracture sites was analyzed.The positive rate between color Doppler ultrasonography and D-dimer were compared and analyzed on the seventh day after operation.The logistic regression analysis was used to analyze the relationship between D-dimer and venous thromboembolism.The value of D-dimer in predicting VTE after bone injury was analyzed using receiver operating characteristic curve. ResultsThe preoperative D-dimer levels in thrombus group and bone injury group were significantly higher than that in control group(P < 0.05), and the postoperative D-dimer level in thrombus group was significantly higher than that in bone injury group(P < 0.01).The positive rates of D-dimer in patients with multiple fractures, pelvic fractures and lower limb fractures were higher than that in patients with upper limb fractures(P < 0.05), and the positive rate in patients with lower limb fractures was significantly higher than that in patients with other fracture sites(P < 0.05).On the seventh day after operation, the positive D-dimer in 24 patients were diagnosed, and 20 patients with venous thromboembolism were diagnosed by color Doppler ultrasound.The results of logistic regression analysis showed that the D-dimer level was an independent predictor of VTE(P < 0.01).The area under the receiver operating characteristic curve of the postoperative D-dimer level predicting VTE after bone trauma was 0.906(95%CI: 0.825-0.987). ConclusionsThe determination of plasma D-dimer level is more sensitive to venous thromboembolism caused by bone injury in patients with bone trauma, which is helpful to predict early thrombosis in patients.
2021, 46(12): 1681-1683, 1687.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.009
Abstract:
ObjectiveTo analyze the predictive value of neutrophil to lymphocyte ratio(NLR) on prognosis of patients with acute myocardial infarction(AMI) combined multi-vessel disease(MVD). MethodsA total of 216 cases of AMI combined with MVD with complete clinical data were included.Patients were divided into high NLR group(60 cases) and low NLR group(156 cases) according to the optimal cut-off value of admission NLR to predict 2-year all-cause mortality.The predictive effect of NLR on the long-term prognosis of AMI patients combined with MVD was analyzed by Cox regression analysis. ResultsThe results of ROC curve showed that the cut-off point value of 2-year all-cause mortality predicted by NLR was 3.65.Compared with patients in the low NLR group, patients in the high NLR group were older and had a higher incidence of hypertension(P < 0.05), and the white blood cell count, neutrophil count and high sensitivity C reactive protein were higher, and the lymphocyte count was lower(P < 0.01).The results of multivariate Cox risk regression analysis showed that NLR, hypertension and neutrophil count were the independent factors affecting all-cause mortality in AMI patients combined with MVD(P < 0.05 to P < 0.01), and NLR and neutrophil count were independent factors affecting the occurrence of MACCE(P < 0.05 and P < 0.01). ConclusionsThe cut-off point value of NLR predicting 2-year all-cause mortality is 3.65.NLR is an independent factor affecting the prognosis of AMI patients combined with MVD.
2021, 46(12): 1684-1687.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.010
Abstract:
ObjectiveTo investigate the effects of platelet-rich fibrin(PRF) in the repair of bone defect around immediate implant teeth. MethodsA total of 74 patients(86 affected teeth) with obvious labial bone plate thinness or bone defect detected by preoperative cone beam CT(CBCT) and conforming to the indications of immediate dental implant were investigated, and divided randomly into the observation group(37 cases, 45 teeth) and control group(37 cases, 41 teeth).The dental implants in the observation group were treated with the PRF bone graft material, oral repair film and PRF film, and the control group was implanted with bone graft material and oral repair film at the same time.The thickness of the top, middle and apex of labial bone plate in sagittal position in two groups were measured before operation.The NobelReplace Conical Connection was immediately implanted, and the affected teeth in two groups were treated with the above surgical procedures, and the wound surface was tightly sutured.Two groups were examined using CBCT at immediate time after surgery and after 6 months of operation, and the thickness and implant stability quotient (ISQ) of the three parts of the labial bone plate in sagittal implant were measured. ResultsThe measurement values of the top, middle and apical part of the labial bone plate in two groups at immediate time after surgery and after 6 months of surgery were higher than those before extraction(P < 0.05).The differences of the measurement value of three parts of labial bone plate before and at immediate time after tooth extraction were not statistically significant between the two groups(P>0.05), but after 6 months of surgery, the measured values of the three parts in observation group were higher than those in control group(P < 0.05).The results of intra-group comparison showed that the ISQ values in the two groups after 6 months of surgery were higher than that at immediate time after surgery(P < 0.05).However, there was no statistical significance in the ISQ values at immediate time and after 6 months of surgery between the two groups(P>0.05). ConclusionsCompared with the traditional repair method, the PRF bone graft material, oral repair film and PRF film can more effectively repair the labial bone defect, which is worthy of clinical promotion.
2021, 46(12): 1688-1691.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.011
Abstract:
ObjectiveTo explore the effects of the treadmill exercise combined with elastic band impedance exercise on the cardiac function in patients with grade Ⅲ heart failure. MethodsA total of 80 patients with grade Ⅲ heart failure from 2018 to 2019 were divided into the control group and observation group according to the random number table method(40 cases in each group).The heart failure in control group was treated with the routine drugs, and the observation group was treated with the treadmill exercise combined with and elastic band impedence exercise on the basis of routine treatment drugs.The 6-min walking distance, cardiac ultrasonic ejection fraction(EF) and n-terminal pro-B-type natriuretic peptide(NT-proBNP) levels in two groups before and after 1 month of treatment were observed to evaluate the levels of cardiac function. ResultsAfter intervention, the 6-min walking distance in two groups increased compared with that before intervention(P < 0.01), and the increasing degree of which in observation group was higher than that in control group(P < 0.05).After intervention, the cardiac ultrasound EF value and NT-proBNP levels in two groups increased and decreased, respectively(P < 0.05), and the increasing degree of EF value and decreasing degree of NT-proBNP levels in observation group were greater than those in control group(P < 0.05 and P < 0.01). ConclusionsThe treadmill exercise combined with elastic band impedence exercise can significantly improve the cardiac function, myocardial contractility and exercise endurance in patients with heart failure.
2021, 46(12): 1691-1694.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.012
Abstract:
ObjectiveTo evaluate the therapeutic effects of complex decongestive therapy(CDT) on breast cancer-related lymphedema. MethodsA total of 73 patients with breast cancer-related lymphedema in The Second Affiliated Hospital of Soochow University from September 2017 to November 2018 were treated with CDT.The circumference and diameter of affected limb edema were measured by upper limb circumference and diameter measurement, and the volume of affected limb was calculated.The reducing degree of circumference and diameter of affected limb were evaluated after treatment.The relationship between treatment effect and duration, body mass index, and effects of affected side and course of lymphedema on the therapeutic effects were analyzed. ResultsAfter CDT, the circumference and diameter of affected limb in 73 cases significantly decreased compared with before treatment(P < 0.01), and the reducing degree of circumference and diameter of affected limb were positively correlated with duration of disease(P < 0.05 to P < 0.01).The differences of the effects of different body mass index, affected side and course of lymphedema on therapeutic effect of lymphedema were not statistically significant(P>0.05). ConclusionsCDT can significantly improve the affected limb edema, and the treatment of full course can significantly improve the treatment effect.CDT is an effective treatment for lymphedema after breast cancer surgery.
2021, 46(12): 1695-1697.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.013
Abstract:
ObjectiveTo investigate the expression of B-cell translocation gene 1 (BTG1) in non-small cell lung cancer(NSCLC) tissue and its correlation with prognosis. MethodsImmunohistochemical method was applied to detect the level of BTG1 protein expression in patients with different clinical pathological parameters of NSCLC.The survival curve was plotted to analyze the relationship between BTG1 and the patient's 5-year survival rate. ResultsThe positive rate of BTG1 protein expression was 38.4% in NSCLC tissues, the lymph node metastasis, clinic stage and histological grade in positive and negative group of BTG1 protein expression were statistically significant(P < 0.05 to P < 0.01).The 5-year survival rate in BTG1 positive group was higher than that in BTG1 negative group, the difference of survival curves in BIG1 positive and negative patients were statistically significant(P < 0.05). ConclusionsDetection of BTG1 expression may be useful in predicting prognosis of NSCLC.BTG1-positive patients have a better prognosis.
2021, 46(12): 1698-1702.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.014
Abstract:
ObjectiveTo evaluate the effects of different doses of dexmedetomidine on the postoperative cognitive dysfunction(POCD) in patients treated with coronary artery bypass transplantation. MethodsOne hundred and twenty patients scheduled by coronary artery bypass transplantation were divided into the different doses of dexmedetomidine group(group D1, group D2 and group D3) and control group(group C) using the random number table method and double-blind method(30 cases in each group).At 30 min before induction of anesthesia, the dexmedetomidine was intravenously administered to group D1, D2 and D3 with loading doses of 0.2, 0.4 and 0.8 μg/kg, respectively, and then at a rate of 0.5 μg·kg-1·h-1 until the end of operation.The group C was given the equal volume 0.9% sodium chloride solution.Before induction of anesthesia after entry(T0), at the beginning of CPB(T1), at the end of CPB(T2), after operation(T3), after 24 h of operation(T4) and after 72 h of operation(T5), the serum levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), neuron-specific enolase(NSE) and central neuron-specific protein(S100β) were detected in all cases, the MMSE scores before 1 day of operation, and after 1, 3 and 7 d of operation were evaluated, and the incidence rate of POCD was calculated. ResultsThe levels of TNF-α, IL-6, NSE and S100β in group D1, group D2 and group D3 were lower than those in group C(P < 0.05), and the incidence rates of POCD in group D1, group D2 and group D3 were significantly lower than those in group C(P < 0.05).The MMSE scores in group D1, group D2, and group D3 after 1 and 3 days of surgery were higher than those in group C(P < 0.05). ConclusionsPreoperative administration of dexmedetomidine can significantly reduce the incidence rate of POCD in patients treated with coronary artery bypass transplantation, which may be closely related to the reduction of inflammatory response, and there is not dose correlation with the incidence rate of POCD at the clinical dose.
2021, 46(12): 1702-1706.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.015
Abstract:
ObjectiveTo explore the relationship between oral ibuprofen in the treatment of patent ductus arteriosus(PDA) and cerebral hemorrhage in very low birth weight infants. MethodsNinety very low birth weight infants with PDA were selected as the research objects.According to the treatment plan, they were divided into study group and control group, with 45 cases in each group.Indomethacin was taken orally in the control group and ibuprofen was taken in the study group.The curative effect, incidence of cerebral hemorrhage and cardiac function[left ventricular ejection fraction(LVEF), left ventricular end diastolic volume(LVEDV), left ventricular end systolic diameter(LVESD)], cerebral hemodynamic parameters[end-diastolic flow velocity(Vd) and resistance index(RI) of the anterior cerebral artery(ACA) and the middle artery(MCA)] before and after treatment in the two groups were counted.Logistic analysis was used to analyze the risk of cerebral hemorrhage in very low birth weight infants with PDA. ResultsThe total effective rate of treatment in the study group was 95.56%, and there was no significant difference compared with 91.11% in the control group(P>0.05).After treatment, the levels of LVEDV, LVESD and LVEF in the two groups were lower than those before treatment(P < 0.05), but there was no significant difference between the two groups(P>0.05);after treatment, the Vd of MCA and ACA in the study group was higher than that in the control group, and the RI was lower than that in the control group(P < 0.01);the incidence of cerebral hemorrhage in the study group was lower than that in the control group(P < 0.05);logistic regression analysis showed that long gestational age, high birth weight, high Apgar score at 1 minute of birth, and ibuprofen treatment were protective factors for cerebral hemorrhage in very low birth weight infants with PDA.Apnea, intrauterine distress, mechanical ventilation, maternal hemorrhage, and delayed labor were risk factors for cerebral hemorrhage in very low birth weight infants with PDA(P < 0.05 to P < 0.01). ConclusionsOral ibuprofen is effective in the treatment of very low birth weight infants with PDA, which can improve ventricular remodeling, stabilize cerebral blood flow, and reduce the incidence of cerebral hemorrhage.In addition, cerebral hemorrhage in very low birth weight infants with PDA is related to various factors such as apnea, intrauterine distress, mechanical ventilation, etc., and craniocerebral ultrasound examination and dynamic observation should be actively performed to further reduce brain damage.
2021, 46(12): 1707-1710, 1714.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.016
Abstract:
ObjectiveTo analyze the risk factors of No.8p lymph metastasis and their effects on long-term prognosis in patients with gastric cancer. MethodsThe clinical data of 106 advanced gastric cancer patients treated with radical operation were analyzed.The patients included 14 cases with No.8p lymph metastasis and 92 cases without No.8p lymph metastasis.The independent risk factors affecting the occurrence of No.8p lymph metastasis were analyzed using multivariate logistic regression analysis, and the effects of No.8p lymph metastasis on the long-term prognosis of patients were analyzed using Cox regression analysis. ResultsThe incidence rate of No.8p lymph metastasis in 106 patients with gastric cancer was 13.21%.Among the patients with No.8p lymph metastasis, there was no statistical significance in the age, gender, smoking, body mass index and drinking(P>0.05), and the differences of the tumor resection site, serum albumin and hemoglobin content were not statistically significant(P>0.05).However, the No.8p lymph metastasis rates in gastric cancer patients with tumor size ≥ 4 cm, higher TNM staging, combined organ resection and intraoperative blood transfusion were higher(P < 0.05).The results of multivariate logistic regression analysis showed that the female, TNM staging and tumor size were the independent risk factors of No.8p lymph metastasis in gastric cancer patients treated with radical operation.A total of 98 patients(92.45%) were followed up for 22 to 86 months(median time for 43 months), and the 5-year survival rate was 39.80%.The 5-year survival rate in patients with No.8p lymph metastasis(13.33%) was lower than that in patients without No.8p lymph metastasis(44.58%)(P < 0.05).The results of multivariate Cox regression analysis showed that tumor size, TNM staging and No.8p lymph metastasis were the independent factors affecting the prognosis of patients(P < 0.01). ConclusionsThe sex, TNM staging and tumor size are the independent risk factors of No.8p lymph metastasis in advanced gastric cancer patients.
2021, 46(12): 1711-1714.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.017
Abstract:
ObjectiveTo investigate the effects of butylphthalide sodium chloride injection on different subtypes of patients with acute cerebral infarction(ACI) in TOAST classification. MethodsOne hundred and eighty patients with ACI were classified according to the TOAST classification standard, and divided randomly into the control group(60 cases) and observation group(120 cases).The control group was treated with the routine medical treatment, and the observation group was additionally treated with butylphthalide sodium chloride injection on the basis of the routine treatment.The NIHSS score and ADL score in the two groups before treatment and after 2 weeks of treatment were recorded, and the total effective rate and efficacy of the same subtype were compared between the two groups. ResultsAfter 2 weeks of treatment, the NIHSS score in observation group(5.50±3.44) points was significantly lower than that in control group(8.02±3.39) points, and the score of ADL in observation group(61.83±9.79) points was significantly higher than that in control group(56.42±10.21) points (P < 0.01).After 2 weeks of treatment, the total effective rate in observation group(88.3%) was higher than that in control group(78.4%), and the overall response rate of type LAA patients was higher than that of type CE and SAO patients in observation group(P < 0.05 to P < 0.01). ConclusionsButylphthalide sodium chloride injection has good effects on patients with ACI, which can significantly improve the symptoms of neurological impairment and self-care ability of patients, and has better effects on LAA patients in TOAST classification.
2021, 46(12): 1715-1718.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.018
Abstract:
ObjectiveTo investigate the effects of the proximal femoral nail antirotation(PFNA) on prognosis of elderly patients with intertrochanteric fracture at different time after injury. MethodsThe clinical data of 142 intertrochanteric fracture patients treated with PFNA were retrospectively analyzed.According to the time from fracture to operation, the patients were divided into the early group(operation within 48 hours after fracture) and elective group(operation after 48 hours of fracture), and followed up for 1 year.The fracture healing time, complications, clinical efficacy and prognosis were compared between the two groups. ResultsThe hospital stay in early group was shorter than that in elective group(P < 0.05), the incidence rate of complications in early group during hospitalization was significantly lower than that in elective group(P < 0.01), and the long-term mortality rate in early group was lower than that in elective group(P < 0.05).There was no significant difference in clinical healing time, short-term mortality and hip joint function at last following up between the two groups(P>0.05). ConclusionsCompared with the elective PFNA surgery, the early surgery can reduce the risk of complications during hospitalization, shorten the length of hospital stay, and reduce the long-term mortality, which has a positive impact on the standardized treatment of elderly hip fracture.Early surgery should be the first choice for patients with good general condition.
2021, 46(12): 1718-1721.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.019
Abstract:
ObjectiveTo study the value of the serum antithrombin-Ⅲ(AT-Ⅲ) combined with modified CT severity index(MCTSI) in the evaluation of severity and prognosis of patients with acute pancreatitis(AP). MethodsOne hundred and twenty-four AP patients were divided into the MAP group(38 cases), MSAP group(46 cases) and SAP group(40 cases) according to the severity of the disease, and the survival group(110 cases) and death group(14 cases) according to the prognosis.The serum levels of AT-Ⅲ and MCTSI scores among the groups were compared.The value of serum AT-Ⅲ combined with MCTSI in the diagnosis of SAP and predicting the death in patients with AP were analyzed using the ROC curve. ResultsThe differences of the serum levels of AT-Ⅲ and MCTSI scores among different disease severity of AP patients were statistically significant(P < 0.01).With the severity of disease increasing, the serum levels of AT-Ⅲ decreased, and the MCTSI scores increased(P < 0.01).The serum level of AT-Ⅲ in death group was significantly lower than that in survival group(P < 0.01), while the MCTSI score was significantly higher than that in survival group(P < 0.01).The AUC, sensitivity and specific degrees of serum AT-Ⅲ combined with MCTSI in the evaluation of AP severity and predicting of death in patients with AP were superior to single detection. ConclusionsThe serum AT-Ⅲ combined with MCTSI in the assessment of disease severity and prognosis of patients with AP has a higher clinical value.
2021, 46(12): 1722-1725.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.020
Abstract:
ObjectiveTo analyze the influencing factors of systemic inflammatory response syndrome (SIRS) after percutaneous nephrolithotomy based on log-binomial regression model. MethodsEighty patients who underwent percutaneous nephrolithotomy were divided into SIRS group(n=23) and control group(n=57).The age, gender, ipsilateral stone operation history, preoperative indwelling double-J tube or not, preoperative urine culture, operation time, lithotomy channel, postoperative blood transfusion or not, stone diameter, postoperative residual stone and stone shape were set as independent variables, and postoperative SIRS or not was set as dependent variable to fit the log-binomial regression model. ResultsUnivariate log-binomial regression analysis showed that preoperative urine culture, operation time, multi-channel lithotomy, postoperative blood transfusion, stone diameter and staghorn stone were the influencing factors of SIRS after percutaneous nephrolithotomy(P < 0.01).The patients with positive urine culture before operation, operation time >60 min, multi-channel lithotomy, postoperative blood transfusion, stone diameter ≥ 50 mm and staghorn stone had higher risk of SIRS(P < 0.01), and the prevalence ratio was 2.14, 2.33, 2.85, 2.65 and 2.59, respectively. ConclusionsThe occurrence of SIRS after percutaneous nephrolithotomy is due to multiple factors.It should be paid more attention to the patients with positive urine culture before operation, operation time >60 min, multi-channel lithotomy, stone diameter ≥ 50 mm and staghorn stone in clinic.
2021, 46(12): 1726-1729.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.021
Abstract:
ObjectiveTo analyze the risk factors of residual symptoms after reduction of benign paroxysmal positional vertigo(BPPV). MethodsA total of 120 BPPV patients with successful reduction were divided into the non-residual group and residual group according to the presence or absence of residual symptoms.The independent risk factors of residual symptoms were analyzed using logistic regression analysis after BPPV reduction.The ROC curve was used to evaluate the predictive value of each index. ResultsThe differences of the gender, etiology, affected side and affected semicircular canal were not statistically significant(P>0.05), while the differences of the age, presence or absence of underlying disease, duration of symptoms, SAS score, number of resets and VNG use were statistically significant between the two groups(P < 0.05).The results of logistic regression analysis showed that the age, basic disease, number of reductions and VNG use were the independent risk factors of residual symptoms after BPPV reduction.The area under ROC curve of each index were 0.778, 0.634, 0.365 and 0.357, respectively. ConclusionsThe patients with underlying diseases and more than 3 times of reduction are prone to have residual symptoms.The residual symptoms in patients applying VGN should be paid close attention to, especial for the elderly.
2021, 46(12): 1729-1733.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.022
Abstract:
ObjectiveTo investigate the relationship between long-term mortality and mean platelet volume(MPV) at the first test in patients with ST-segment elevation myocardial infarction(STEMI). MethodsA total of 193 STEMI patients were followed up until October 8, 2019 after STEMI.According to the results of following up, the patients were divided into the survival group and death group.The blood routine indexes between the two groups were compared.The relationship between the blood routine indexes and long-term prognosis of STEMI was analyzed using multiple linear regression analysis, the cut-off value of MPV to long-term mortality of STEMI was analyzed using ROC analysis, the survival analysis was conducted by Kaplan-Meier curve, and the Log-Rank test was used. ResultsThe results of blood routine indicators analysis showed that the levels of neutrophils, eosinophils, basophils and MPV in survival group were significantly lower than those in death group(P < 0.05 to P < 0.01).The cut-off value of MPV predicting long-term death in STEMI patients was 13.14 fL, the AUC was 0.741, the 95%CI was 0.658-0.824, the sensitivity was 78.94%(30/38), the specificity was 83.33%(105/126), the accuracy was 82.32% (135/164), the positive predictive value was 58.82% (30/51), and the negative predictive value was 92.92%(105/113).The results of ROC analysis showed that 8 patients died in the low-level MPV group(113 cases, MPV < 13.14 fL), and 30 patients died in the high level MPV group(51 cases, MPV ≥ 13.14 fL).The results of Kaplan-Meier analysis showed that the median survival time in low-level MPV group was 33 months(95%CI: 31.09-34.26), which was higher than that in high-level MPV group[25 months, (95%CI: 22.13-27.29)] (P < 0.01).The incidence rates of cerebrovascular events in low-level MPV group(24.78%) was lower than that in high-level MPV group(76.47%)(P < 0.01).Among 193 STEMI patients, 98 patients (50.78%) with different degrees of myocardial ischemia reperfusion injury were identified, and the level of MPV in patients with myocardial ischemia reperfusion injury was higher than that in patients without myocardial ischemia reperfusion injury(P < 0.01). ConclusionsThe level of MPV in STEMI patients is significantly higher than that in healthy people at the first test, and the MPV can be used as an indicator for the long-term prognosis of STEMI patients.When the MPV is more than or equal to 13.14 fL, the risk of long-term death in patients with STEMI significantly increases, and the risk of myocardial ischemia reperfusion increases with the increasing of MPV level.
2021, 46(12): 1734-1736.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.023
Abstract:
ObjectiveTo compare the effects of ropivacaine and bupivacaine on urinary tract function in patients with thoracic epidural analgesia(TEA) by monitoring residual urine volume and maximum urine flow. MethodsFifty patients undergoing elective open thoracic and abdominal surgery were selected and divided into group A(bupivacaine analgesic group) and group B(ropivacaine analgesic group) by random number table method, with 25 patients in each group.Urodynamic examination was performed in preoperative and TEA.The primary observation index was the difference of postoperative residual urine volume(PVR) before and after TEA, and the secondary observation index was the change of maximum urine flow between groups and within groups. ResultsDuring TEA, PVR in both groups increased compared with the baseline value(P < 0.05), and PVR in group A was higher than that in group B(P < 0.01).The urine output in group A was higher than that in group B during TEA(P < 0.01).During TEA, the maximum urine flow and bladder contraction index in the two groups were decreased compared with the baseline value(P < 0.05), and which in group A was lower than that in group B(P < 0.01).There were no significant differences in baseline value, initial urine intention, strong urine intention, maximum bladder capacity and bladder compliance parameters between the two groups during TEA(P>0.05). ConclusionsThe residual urine volume of ropivacaine for TEA is significantly lower than that of bupivacaine, which reflects that ropivacaine has little effect on detrusor function.
2021, 46(12): 1737-1740.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.024
Abstract:
ObjectiveTo investigate the risk factors of nosocomial infection in peritoneal dialysis(PD) patients with chronic renal failure. MethodsThe clinical data of 193 PD patients with chronic renal failure were retrospectively analyzed, and divided into the infection group and non-infection group according to whether occurrence of nosocomial infection.The nosocomial infection situation and distribution of pathogenic bacteria in two groups were analyzed.The clinical data were compared between two groups, and the risk factors of nosocomial infection in PD patients were analyzed using multivariate logistic analysis. ResultsFifty-two PD patients(21.76%) with nosocomial infection were found, which included 17 cases with respiratory tract infections(8.81%) and 10 cases with urinary tract infections(5.18%).A total of 56 strains pathogenic bacteria were cultured, which included 44 strains of gram-negative strains(78.57%) and 5 strains of gram-positive strains(8.93%).The differences of the age, length of hospital stay, dialysis duration, hypoproteinemia, body mass index(BMI), anemia, cardiac insufficiency, full hemodialysis and central venous catheter application between two groups group were statistically significant(P < 0.05 to P < 0.01).The age, length of hospital stay, length of dialysis, hypoproteinemia, BMI, anemia, cardiac insufficiency, adequate hemodialysis and use of central venous catheter were the independent risk factors of nosocomial infection(P < 0.01). ConclusionsThe respiratory tract infection and Klebsiella pneumoniae are the most common nosocomial infections in PD patients with chronic renal failure.Clinical attention should be paid to PD patients with advanced age, long hospitalization, dialysis, hypoproteinemia, anemia and cardiac insufficiency to prevent nosocomial infections.
2021, 46(12): 1741-1743, 1747.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.025
Abstract:
ObjectiveTo explore the common attention of children with language retardation(LR) caused by autism spectrum disorder(ASD) and mental retardation. MethodsForty children with ASD and 40 children with LR were divided into the ASD group and LR grouop, and 40 children with healthy physical examination at the same period were set as the control group.The early social communication scale was used to evaluate the common attention of three groups. ResultsThe results of the comparison of common attention showed that the scores of responding joint attention(RJA), initiating joint attention(IJA), higher level initiating joint attention(HIJA), lower level initiating joint attention(LIJA), alternate(Alt) and eye contact(EC) in LR group were lower than those in control group(P < 0.01), and the scores of RJA, higher level responding joint attention(HRJA), lower level responding joint attention(LRJA), IJA, HIJA, LIJA, Alt and EC in ASD group were lower than those in control group(P < 0.01).The scores of RJA, HRJA, LRJA, IJA, LIJA and EC in LR group were higher than those in ASD group(P < 0.01), but the differences of the scores of HIJA, Show, Point and Alt between LR group and ASD group were not statistically significant(P>0.05). ConclusionsBoth ASD and LR children have obvious common attention deficit, but the eye contact behavioral defects in LR children are mainly manifested, which can be used for the preliminary diagnosis of ASD in clinic.
2021, 46(12): 1744-1747.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.026
Abstract:
ObjectiveTo investigate the efficacy of Ginkgo Folium extract combined with hyperbaric oxygen in the treatment of Alzheimer's disease in the elderly. MethodsNinety-eight patients with Alzheimer's disease were randomly divided into the control group and observation group(49 cases in each group).The control group was treated with Ginkgo Folium extract, and the observation group was treated with Ginkgo Folium extract combined with hyperbaric oxygen therapy.The clinical efficacy, serum index and incidence rate of adverse reactions were compared between the two groups. ResultsAfter treatment, the MMSE score and ADL score in observation group were higher than those in control group, while the ADAS-Cog score in observation group was lower than that in control group(P < 0.01).After treatment, the levels of SOD, GSH-Px and TGF-β1 in observation group were higher than those in control group, and the levels of MDA and IL-1β were lower than those in the control group(P < 0.01).The differences of the incidence rates of fatigue, nausea, dizziness, insomnia and rash between the two groups were not statistically significant(P>0.05). ConclusionsGinkgo Folium extract combined with hyperbaric oxygen in the treatment of elderly Alzheimer's disease can effectively improve clinical efficacy and serum related indicators, and has fewer adverse reactions, which is worthy of clinical promotion.
2021, 46(12): 1748-1750, 1756.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.027
Abstract:
ObjectiveTo investigate the application value of styrofoam combined with head and neck shoulder thermoplastic film in position fixation of nasopharyngeal carcinoma treated with helical tomography intensity modulated radiotherapy. MethodsTwenty nasopharyngeal carcinoma patients treated with CT radiotherapy were randomly divided into the control group and observation group(10 cases in each group).The control group was fixed with regular headrest combined with head and neck shoulder thermoplstic film, and the observation group was fixed with styrofoam combined with head and neck shoulder thermoplastic film.The image was obtained by MVCT scan before treatment, and registered with the image of simulated CT, the pendulum errors of X(left and right), Y(corner), Z(ventral dorsal) and ROLL(rotation along cross section) axis were recorded and analyzed. ResultsThe pendulum errors of the direction of X, Y, Z and ROLL axises in the control group were(1.58±1.14)mm, (1.44±1.29)mm, (3.19±1.26)mm and(1.16±1.09)°, respectively.The pendulum errors of the direction of X, Y, Z and ROLL axises were(1.06±0.89)mm, (1.02±0.73)mm, (2.71±1.37) mm and(1.31±1.12)°, respectively.The differences of pendulum errors in X, Y and Z axis directions between the two groups were statistically significant(P < 0.01), and the difference of the pendulum error in ROLL axis direction between the two groups was not statistically significant(P>0.05).The pendulum error in the direction of X, Y and Z axis in the control group and observation group mainly concentrated in 0-6 mm and 0-4 mm, respectively.The cross-sectional rotation error distribution at the interval of 0°~, 1.1°~and 2.1°~in observation group was 134(48.2%), 89(32.0%) and 55(19.8%), respectively.The cross-sectional rotation error distribution at the interval of 0°~, 1.1°~and 2.1°~in control group was 177(59.8%), 60(20.3%), and 59(19.9%), respectively. ConclusionsIn order to reduce the pendulum error, it is recommended to use styrofoam combined with thermoplastic film for the fixation of nasopharyngeal carcinoma patients.
2021, 46(12): 1751-1756.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.028
Abstract:
ObjectiveTo investigate the correlations between serum intercellular adhesion molecule-1(ICAM-1), hypersensitive C reactive protein(hs-CRP), monocyte chemoattractant protein-1(MCP-1) and carotid artery plaque in patients with acute cerebral infarction(ACI). MethodsOne hundred and five ACI patients were admitted, the patients' clinical data were collected, the levels of serum hs-CRP and ICAM-1 were detected by ELISA and serum MCP-1 level was detected by double antibody sandwich ELISA, and the carotid intima-media thickness(IMT) and the characteristics of carotid artery plaque were measured by carotid ultrasound.The patients were subdivided into 0.9 mm < IMT ≤ 1.2 mm group, 1.2 mm < IMT ≤ 1.4 mm group, IMT>1.4 mm group, and also divided into the stability plaque group(39 cases) and vulnerable plaque group(66 cases) according to the characteristics of the plaques.The correlations between serum hs-CRP, ICAM-1, MCP-1 and IMT were analyzed by Pearson correlation analysis.The risk factors influencing the ACI carotid atherosclerotic plaque instability were analyzed by multiple logistic regression analysis, and the value of serum hs-CRP, ICAM-1, MCP-1 levels in predicting the carotid atherosclerotic plaque instability in ACI patients were analyzed by the receiver operating characteristic(ROC) curve. ResultsThe levels of serum hs-CRP, ICAM-1 and MCP-1 in vulnerable plaques group were higher than those in stability plaque group(P < 0.01).The levels of serum hs-CRP, ICAM-1 and MCP-1 were increased successively in the 0.9 mm < IMT ≤ 1.2 mm group, 1.2 mm < IMT ≤ 1.4 mm group, and IMT>1.4 mm group(P < 0.05 to P < 0.01).Pearson correlation analysis showed that serum hs-CRP, ICAM-1 and MCP-1 levels were positively correlated with IMT(r=0.495, 0.585, 0.498, P < 0.05).Multiple logistic regression analysis showed that the high levels of ICAM-1, hs-CRP, MCP-1, LDL-C and diabetes mellitus were the risk factors for carotid plaque instability in ACI patients(P < 0.01).ROC analysis showed that serum hs-CRP, ICAM-1 and MCP-1 levels and the combinations of the three factors which predicted the area under the curve(AUC) of carotid atherosclerotic plaque instability in ACI patients were 0.722, 0.753, 0.646 and 0.852, respectively.The sensitivity was 69.23%, 79.49%, 66.67% and 89.74%, and the specificity was 74.24%, 68.18%, 65.15% and 89.39%, respectively. ConclusionsSerum ICAM-1, hs-CRP and MCP-1 levels are related to the characteristics of carotid atherosclerotic plaque in ACI patients, which may be used as the predictors of carotid plaque instability.
2021, 46(12): 1757-1760.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.029
Abstract:
ObjectiveTo investigate the anesthetic effects of different doses of ropivacaine combined with sufentanil in cesarean section with equal specific gravity spinal anesthesia. MethodsEighty parturients with full-term cesarean section(ASA Ⅰ-Ⅱ) were randomly divided into the group A(n=27), group B(n=27) and group C(n=26).The group A, group B and group C were anesthetized with 10 mg of 0.5% ropivacaine hydrochloride combined with 5 μg of sufentanil, 11.25 mg of 0.5% ropivacaine hydrochloride combined with 5 μg of sufentanil and 12.5 mg of 0.5% ropivacaine hydrochloride combined with 5 μg of sufentanil, respectively. ResultsThe differences of the effective time of anesthesia block, and proportion and time of block level above T8 among three groups were not statistically significant(P>0.05), and the differences of the classification of anesthetic effects among three groups were statistically significant(P < 0.01).The proportion of intraoperative use of hyperensort in group C was higher than that in group A and group B(P < 0.05).The differences of the interaction effects of neonatal Apgar scores with time factor and group factor, and interaction effects of time factor with group factor among three groups were not statistically significant(P>0.05). ConclusionsWhen ropivacaine combined with sufentanil in equal specific gravity spinal anesthesia during cesarean section, the appropriate dose of ropivacaine hydrochloride for 11.25 mg can meet the needs of rapid onset and good effects of anesthesia.
2021, 46(12): 1763-1767.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.031
Abstract:
ObjectiveTo investigate the value of T2WI combined with DCE-MRI radiomics features in the preoperative prediction of axillary lymph node metastasis of invasive breast cancer. MethodsThe clinicopathological and MRI data of 168 patients with invasive breast cancer confirmed by surgical pathology were retrospectively analyzed. According to the pathological results, the patients were divided into the lymph node metastasis group(n=64) and non-lymph node metastasis group(n=104), and randomly divided into the training group(n=134) and verification group(n=34) in an 8:2 ratio. The ROI was manually delineated on T2WI and DCE sequences for image segmentation and image omics feature extraction. The Select K Best, LASSO regression and iterative screening features were used to reduce the dimensionality of high-dimensional omics features and retain the high associated features with axillary lymph node metastasis. The three imaging omics prediction models of T2WI, DCE and T2WI combined WITH DCE were established using the logistic regression. The area under the ROC curve(AUC) was used to evaluate the effectiveness of models, and the optimal model was used to generate a column chart. ResultsThe AUC of T2WI, DCE, and T2WI combined with DCE in the training group was 0.75, 0.75, 0.80, respectively. The AUC of T2WI, DCE, and T2WI combined with DCE in the validation group was 0.75, 0.73 and 0.79, respectively. The predictive performance in T2WI combined with DCE predictive model was the best. ConclusionsThe predictive model of T2WI combined with DCE has certain value in the preoperative prediction of axillary lymph node metastasis of invasive breast cancer. It can accurately and noninvasively predict the status of axillary lymph node metastasis.
2021, 46(12): 1768-1771.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.032
Abstract:
ObjectiveTo evaluate the application value of two-dimensional transvaginal ultrasonography(2D-TVS), three-dimensional transvaginal ultrasonography(3D-TVS) volumetric imaging and three-dimensional Doppler ultrasound(3D-DUS) in the diagnosis and initial location of submucous myoma of prolapsed type. MethodsFifty-seven patients with suspected prolapsed submucosal myoma deciding on surgical treatment were detected using 2D-TVS and 3D-TVS before operation. Taking the surgery results as golden standard, the diagnostic accuracy was compared between the two methods. According to the surgical results, the patients with protuberant submucosal myoma and patients with non-myomatous disease were divided into the confirmed group and suspected group, respectively. The ultrasonographic characteristics of "low echo" and "Ⅰ shaped pedicle structure" were compared between two groups. ResultsThere were 34 cases of prolapsive submucosal myoma, 20 cases of cervical polyp, 1 case of cervical cancer, 1 case of endometrial cancer and 1 case of cervical myom according to the results of the surgery and pathology. The diagnostic sensitivity of 2D-TVS and 3D-TVS was 88.2% and 94.1%, respectively, and the difference of which was not statistically significant(P > 0.05). The specificity of 2D-TVS and 3D-TVS was 54.5% and 86.4%, respectively, and which of 3D-TVS was higher than that of 2D-TVS(P < 0.05). The percentage of consistency in 2D-TVS and 3D-TVS was 75% and 91%, respectively. In terms of the low echo, "Ⅰ shaped" pedicle structure and rich strip blood flow in 2D-TVS, and pear-shaped" mass of 3D-TVS and strip bundle blood flow of 3D-DUS, the detection rate of which in confirmed group was higher than that in suspected group(P < 0.05 to P < 0.01). Conclusions2D-TVS and 3D-TVS can be used to diagnose the submucous myoma of prolapsed type, and the diagnostic specificity of the 3D-TVS is higher than that of the 2D-TVS. The sonographic characteristics of tumor morphology and blood flow displayed by 2D-TVS, 3D-TVS and 3D-DUS can be used as the diagnostic and differential diagnostic indicators, which can improve the diagnostic accuracy and reduce misdiagnosis and missed diagnosis.
2021, 46(12): 1772-1776.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.033
Abstract:
ObjectiveTo investigate the application value of the fuzzy comprehensive evaluation method in the evaluation of nursing quality of emergency department nursing staff. MethodsThe Delphi method was used to construct the index evaluation system of nursing quality in emergency department, and the nursing quality in emergency department of a hospital was quantitatively evaluated using the multi-level fuzzy comprehensive evaluation method. ResultsThe index and weight of emergency department nursing quality evaluation system were established using Delphi method and analytic hierarchy process, and the emergency department nursing quality was scientifically evaluated using the fuzzy comprehensive evaluation method. The results of the nursing quality of the emergency department of the hospital showed that the rated as "excellent", "good", "general" and "poor" were 32.0%, 27.2%, 22.7% and 18.1%, respectively. The sum of excellent ratio was 59.2%, which indicated that the nursing quality of the emergency department should be continuously improved. ConclusionsThe fuzzy comprehensive evaluation can effectively transform the quantitative indexes into qualitative indexes, overcome subjective factors, and help to clarify the direction of improving emergency department nursing quality.
2021, 46(12): 1776-1779.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.034
Abstract:
ObjectiveTo explore the effect of medical care-patient-management three-dimensional integrated management mode on medication compliance, self-management properties and functional independence of patients with postoperative cerebral hemorrhage. MethodsA total of 102 patients with postoperative cerebral hemorrhage were selected and randomly divided into control group and observation group, with 51 cases in each group. The control group received routine management of postoperative cerebral hemorrhage, while the observation group received a three-dimensional integrated management of medical care-patient-management. The medication compliance, self-management property, quality of life, and functional independence were compared between the two groups. ResultsThe rate of better medication compliance in the observation group was higher than that in the control group(P < 0.01). Compared with the control group, the observation group had higher control rates of dietary structure, exercise mode, blood pressure, blood glucose and blood lipids(P < 0.01). Moreover, the scores of postoperative quality of life and functional independence scale in the observation group were also shown higher than those in the control group(P < 0.01). ConclusionsApplying the medical care-patient-management three-dimensional integrated management mode to patients with postoperative cerebral hemorrhage can effectively improve patients' medication compliance, recovery of self-management ability, functional independence, and quality of life.
2021, 46(12): 1780-1782.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.035
Abstract:
ObjectiveTo design the new drainage and seepage collecting device, and observe its application effects in wound exudation of patients treated with gynecological surgery. MethodsThirty patients with wound or drainage orifice exudation after gynecological surgery were investigated, and the drainage effects of new drainage and seepage collecting device were observed. ResultsThe healing time and extubation time, and number of dressing change in 30 patients treated with drainage and seepage collecting device shortened by 2 to 3 d and 1 to 2 d, and reduced to 0 to 1 time, respectively. Among the patients, the mobility was convenient, the comfort increased, and the rehabilitation was accelerated. ConclusionsThe use of a drainage and seepage collecting device can shorten the wound healing time, prevent skin inflammation, reduce hospital infection, save medical cost, and improve the comfort and satisfaction with medical treatment and nursing of patients.
2021, 46(12): 1785-1787, 1791.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.037
Abstract:
ObjectiveTo study the application value of early progressive rehabilitation training in severe patients with mechanical ventilation. MethodsNinety-two patients with severe mechanical ventilation were randomly divided into the observation group and control group(46 cases in each group). The control group was treated with conventional mechanical ventilation intervention, and the observation group was treated with early progressive rehabilitation training on the basis of the control group. The hospitalization status in the groups were observed and recorded. The functional independence levels in two groups were evaluated using the functional independence scale(FIM) after tracheal intubation removal, ICU transfer and discharge, and the incidence rate of complications was compared between the two groups. ResultsThe mechanical ventilation time, ICU monitoring time and discharge time in observation group were significantly shorter than those in control group(P < 0.01). The total score of FIM at tracheal intubation removal, ICU transfer and discharge in observation group were significantly higher than those in control group(P < 0.01). The total incidence rate of complications in observation group was less than that in control group(P < 0.05). ConclusionsEarly progressive rehabilitation training can effectively improve patients' muscle and nerve function, improve patients' functional independence, and reduce the incidence rate of complications.
2021, 46(12): 1788-1791.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.038
Abstract:
ObjectiveTo investigate the current situation of medical device-related pressure ulcer in the stoma care clinic, and to analyze the causes and discuss the countermeasures. MethodsA total of 949 cases of enterostomy patients who were followed up for the first time in the stoma care clinic were selected. The clinical data of patients with medical device-related pressure ulcer were retrospectively analyzed to investigate the current situation, look for the causes and provide targeted measures. ResultsThere were 76 cases of medical device-related pressure ulcer in different stages, the incidence was 8.01%. Among them, there were 38 cases of ostomy belt correlation, the incidence was 8.54%; 20 cases of rubber support correlation, the incidence was 75.92%; 12 cases of ostomy barrier correlation, the incidence was 1.28%; 4 cases of tail clip correlation, the incidence was 0.47%. There were 2 cases with other causes, and the incidence was 0.21%. There was no significant difference in the causes of medical device-related pressure ulcer in different stages. A total of 76 cases with 1-3 stage of wounds were healed, with healing time of 3-17 d. The main reasons were that the nurses' awareness of medical device-related pressure ulcer was not strong, the patients and caregivers did not have adequate health education and knowledge, and medical device material and local microenvironment changed. ConclusionsThe prevention of medical device-related pressure ulcer is the most important. It is necessary to strengthen the training of the related knowledge for the nurses. The early intervention and making personal health education are also essential. Appropriate dressings and measures should be selected to treat the wounds in order to promote the wound healing according to the principle of TIME.
2021, 46(12): 1792-1795.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.039
Abstract:
ObjectiveTo investigate the application effects of self-made cervical traction pad in the skull traction treatment of cervical fracture complicated with dislocation. MethodsA total of 64 patients with cervical vertebra fracture complicated with dislocation caused by exceptional injury and requiring skull traction treatment in the Department of Orthopedics Spinal Trauma in Fuyang People's Hospital of Anhui province from January 2016 to February 2020 were investigated, and randomly divided into the observation group and control group(32 cases in each group). The control group was treated with the traditional towel roll to pad the neck and quilts or bath towels to pad the shoulder and back for raising the chest and back during skull traction. The observation group was treated with the self-designed cervical traction pad. The SF-McGill scale was used to evaluate the pain degree, incidence of stage Ⅰ stress injury and comfort level of patients on the next day, fourth day and last observation during traction treatment, and the differences of which were statistically analyzed. ResultsThe difference of the SF-McGill scale score on the second day of traction was not statistically between two groups(P > 0.05), and the SF-McGill scale scores in observation group on the fourth day and last observation of traction were lower than that in control group(P < 0.01). The incidence rate of stress injury and comfort score in observation group were lower and higher than that in control group, respectively(P < 0.01). ConclusionsCompared with traditional filling, the self-made cervical traction pad can reduce the pain degree and occurrence of skin pressure injury, and improve the comfort level of patients in the process of traction.
2021, 46(12): 1796-1799.
doi: 10.13898/j.cnki.issn.1000-2200.2021.12.040
Abstract:
ObjectiveTo explore the application effect of diversified teaching model based on clinical cases in the teaching of obstetrics and gynecology nursing. MethodsThe control group and experimental group were taught using the traditional teaching mode and diversified teaching mode based on clinical cases, respectively. The teaching effects in two groups were evaluated using the questionnaire survey and course examination score. ResultsThe score in experimental group was significantly higher than that in control group(P < 0.01). One hundred and six students(91.4%) believed that the diversified teaching mode based on clinical cases could improve the overall teaching effect and 108 students(93.1%) wanted to continue the diversified teaching mode. ConclusionsThe diversified teaching mode based on clinical cases is feasible, which is helpful for the cultivation of students' comprehensive ability.
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