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2004 Vol. 29, No. 1
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2004, 29(1): 1-4.
Abstract:
Objective: To investigate the antitumor effect and possible mechanism of AC-88.Methods: H22,Meth A,EAC cell bearing mice in vivo and LOVO,TYK,H22 culture cells in vitro were used to study the antitumor effect of AC-88 and Western-blotting was used to study Fas expression of T lymphocytes.Results: The antitumor rate of AC-88 250 mg/kg group on three types tumor bearing mice was 76.31%,73.52%,and 71.91%,respectively.The average tumor weight was lower than control group(P<0.01).The IC 50 of AC-88 on each type tumor cells was lower than 10 μg/ml in vitro.Fas expression of T cells was decreased in the same time(P<0.01).Conclusions: AC-88 has obvious antitumor effect and maybe through decrease T cell Fas expression.
Objective: To investigate the antitumor effect and possible mechanism of AC-88.Methods: H22,Meth A,EAC cell bearing mice in vivo and LOVO,TYK,H22 culture cells in vitro were used to study the antitumor effect of AC-88 and Western-blotting was used to study Fas expression of T lymphocytes.Results: The antitumor rate of AC-88 250 mg/kg group on three types tumor bearing mice was 76.31%,73.52%,and 71.91%,respectively.The average tumor weight was lower than control group(P<0.01).The IC 50 of AC-88 on each type tumor cells was lower than 10 μg/ml in vitro.Fas expression of T cells was decreased in the same time(P<0.01).Conclusions: AC-88 has obvious antitumor effect and maybe through decrease T cell Fas expression.
2004, 29(1): 5-8.
Abstract:
Objective: To investigate the effect of nitric oxide in preventing postoperative adhesions formation in traumatic mice.Methods: In the pre experimental group eight kunming mice were created cecal abrasion and parietal peritoneal defect with vefined Mazuji methods as experimental models and the effect of adhesion formation was observed.In the experimental group,seventy-two mice were divided into control group(Ⅰ),L-arginine group(Ⅱ) and NG nitroarginine methyl ester(L-NAME,Ⅲ) group.0.9% NaCl(Ⅰ),L-arginine(Ⅱ) and L-NAME(Ⅲ) were administered intraperitoneally before abdominal closure and 3 consecutive days after surgery.All the three groups were divided into three subgroups for evaluation of adhesion severity and serum nitric oxide(NO) levels at postoperative days 3,7 and 14.Adhesion severity was measured with adhesion grading(Nairs's standard)and tissue hydroxyproline(OHP) levels.Results: In the pre-experimental group,the adhesion rate was 100% in the eight mice two weeks after operation.All adhesions were middle or severe adhesion(3 to 4 grade) and the mean score was 3.50±0.53.In the experimental group,(1)the adhesion mean score and OHP levels were minimal and almost equal between the three groups at day 3(subgroup A)(P>0.05),while the adhesion mean score and OHP levels were higher in groupⅠand Ⅲ than in group Ⅱ at day 7(subgroup B) and 14(subgroup C)(P<0.01).The adhesion mean score and OHP levels were the highest in subgroup A,the lowest in subgroup C and the medium in subgroup B(P<0.01).Logistic regression analysis showed significant correlation between adhesion score and tissue OHP levels in subgroup B and C respectively(rb=0.95, rc=0.93, P<0.01).(2)NO level was higher in group Ⅱ than in groupⅠand group Ⅲ at day 3,7 and 14 respectively(P<0.01)and there was no difference between groupⅠand group Ⅲ(P>0.05).There was no difference between subgroup A,B and C(P>0.05).Conclusions: The intraperitoneal administration of L-arginine results in significantly higher serum nitric oxide levels and inhibits postoperative adhesion formation.
Objective: To investigate the effect of nitric oxide in preventing postoperative adhesions formation in traumatic mice.Methods: In the pre experimental group eight kunming mice were created cecal abrasion and parietal peritoneal defect with vefined Mazuji methods as experimental models and the effect of adhesion formation was observed.In the experimental group,seventy-two mice were divided into control group(Ⅰ),L-arginine group(Ⅱ) and NG nitroarginine methyl ester(L-NAME,Ⅲ) group.0.9% NaCl(Ⅰ),L-arginine(Ⅱ) and L-NAME(Ⅲ) were administered intraperitoneally before abdominal closure and 3 consecutive days after surgery.All the three groups were divided into three subgroups for evaluation of adhesion severity and serum nitric oxide(NO) levels at postoperative days 3,7 and 14.Adhesion severity was measured with adhesion grading(Nairs's standard)and tissue hydroxyproline(OHP) levels.Results: In the pre-experimental group,the adhesion rate was 100% in the eight mice two weeks after operation.All adhesions were middle or severe adhesion(3 to 4 grade) and the mean score was 3.50±0.53.In the experimental group,(1)the adhesion mean score and OHP levels were minimal and almost equal between the three groups at day 3(subgroup A)(P>0.05),while the adhesion mean score and OHP levels were higher in groupⅠand Ⅲ than in group Ⅱ at day 7(subgroup B) and 14(subgroup C)(P<0.01).The adhesion mean score and OHP levels were the highest in subgroup A,the lowest in subgroup C and the medium in subgroup B(P<0.01).Logistic regression analysis showed significant correlation between adhesion score and tissue OHP levels in subgroup B and C respectively(rb=0.95, rc=0.93, P<0.01).(2)NO level was higher in group Ⅱ than in groupⅠand group Ⅲ at day 3,7 and 14 respectively(P<0.01)and there was no difference between groupⅠand group Ⅲ(P>0.05).There was no difference between subgroup A,B and C(P>0.05).Conclusions: The intraperitoneal administration of L-arginine results in significantly higher serum nitric oxide levels and inhibits postoperative adhesion formation.
2004, 29(1): 9-11.
Abstract:
Objective: To study the influence of kainate on the hippocampal neuron of rats and the expression of Bax/bcl-2.Methods: The rats were divided into two groups random ly.The ex perimental groupw as given intraperitoneal administration of kainate, and the control groupw as given phosphate-buffered saline solution (PBS).After 3 days, the loss of neuron in the hippocampus of rats was detected by Nissl staining.The neuronic apoptosis in the hippocampus was examined with terminal deoxy nucleotidyl-transferase mediated d-U TP-biotin nick end labeling (TUN EL);related gene Bax and Bcl-2 expression were marked by SABC method.Results: Kainate could induce neuron apoptosis and loss, up-regulation of bax and down-reg ulation of bcl-2 in the hippocampus of rats by intraperitoneal administration.Conclusions: The hippocampal neuron of rats can be impaired by intraperitoneal administration of kainate.Elevated expression of Bax/bcl-2 of neuron mig ht be involved in the pathophysiologic course of apoptosis in neurons.
Objective: To study the influence of kainate on the hippocampal neuron of rats and the expression of Bax/bcl-2.Methods: The rats were divided into two groups random ly.The ex perimental groupw as given intraperitoneal administration of kainate, and the control groupw as given phosphate-buffered saline solution (PBS).After 3 days, the loss of neuron in the hippocampus of rats was detected by Nissl staining.The neuronic apoptosis in the hippocampus was examined with terminal deoxy nucleotidyl-transferase mediated d-U TP-biotin nick end labeling (TUN EL);related gene Bax and Bcl-2 expression were marked by SABC method.Results: Kainate could induce neuron apoptosis and loss, up-regulation of bax and down-reg ulation of bcl-2 in the hippocampus of rats by intraperitoneal administration.Conclusions: The hippocampal neuron of rats can be impaired by intraperitoneal administration of kainate.Elevated expression of Bax/bcl-2 of neuron mig ht be involved in the pathophysiologic course of apoptosis in neurons.
2004, 29(1): 12-13.
Abstract:
Objective: To study the effect of rhGH on renal carcinoma cells line, so as to improve the treatment results.Methods: GRC-1 cells were cultured respectively for 24 hour in the control group, the rhGH group, the 5-Fu group and the rhGH +5-Fu group.The number of different phase cells was determined by flow cytometry (FCM).Results: The cells in G0 to G1 phase and S phase accounted for 53.4% and 42.1% respectively in the control group, but 41.1% and 52.7% in the rhGH group.The difference was significant(P<0.01 and P<0.05).In the 5-Fu group, the number of cells increased in G0 to G1 phase(62.3%), while it decreased in S phase (37.5%).In the rhGH+5-Fu group, the number of cells increased further in G0 to G1 phase(67.4%), while it decreased further in S phase (32.5%).There was no obvious difference in the two groups (P>0.05).Conclusions: rhGH may induce differentiation of GRC-1 cells but can not strengthen Fluorouraci in suppressing GRC-1 cells.
Objective: To study the effect of rhGH on renal carcinoma cells line, so as to improve the treatment results.Methods: GRC-1 cells were cultured respectively for 24 hour in the control group, the rhGH group, the 5-Fu group and the rhGH +5-Fu group.The number of different phase cells was determined by flow cytometry (FCM).Results: The cells in G0 to G1 phase and S phase accounted for 53.4% and 42.1% respectively in the control group, but 41.1% and 52.7% in the rhGH group.The difference was significant(P<0.01 and P<0.05).In the 5-Fu group, the number of cells increased in G0 to G1 phase(62.3%), while it decreased in S phase (37.5%).In the rhGH+5-Fu group, the number of cells increased further in G0 to G1 phase(67.4%), while it decreased further in S phase (32.5%).There was no obvious difference in the two groups (P>0.05).Conclusions: rhGH may induce differentiation of GRC-1 cells but can not strengthen Fluorouraci in suppressing GRC-1 cells.
2004, 29(1): 14-15.
Abstract:
Objective: To observe the effects of high concentration of glucose on the auto-rhythmicity of the smooth muscle of rabbit duodenum.Methods: Duodenum of rabbits were isolated and put into normal Tyrode solutions.Tyrode solutions coutaining high glucose(9.7 mmol/L and 11.1 mmol/L),and then the curve of contraction was recorded.Results: The contraction amplitude of the duodenum in Tyrode solution containing glucose concentrations 9.7 mmol/L was distinctly inhibited at 10 min(P<0.05),while that in Tyrode solution containing glucose of 11.1 mmol/L was distictly inhibited at 20 min(P<0.05);and then the contraction rose gradually.Conclusions: High concentration of glucose may make the contraction of duodenum inhibited.When the glucose concentrations in Tyrode solution increased,the time when the contraction of the duodenum was inhibited was prolonged.
Objective: To observe the effects of high concentration of glucose on the auto-rhythmicity of the smooth muscle of rabbit duodenum.Methods: Duodenum of rabbits were isolated and put into normal Tyrode solutions.Tyrode solutions coutaining high glucose(9.7 mmol/L and 11.1 mmol/L),and then the curve of contraction was recorded.Results: The contraction amplitude of the duodenum in Tyrode solution containing glucose concentrations 9.7 mmol/L was distinctly inhibited at 10 min(P<0.05),while that in Tyrode solution containing glucose of 11.1 mmol/L was distictly inhibited at 20 min(P<0.05);and then the contraction rose gradually.Conclusions: High concentration of glucose may make the contraction of duodenum inhibited.When the glucose concentrations in Tyrode solution increased,the time when the contraction of the duodenum was inhibited was prolonged.
2004, 29(1): 16-18.
Abstract:
Objective: To observe the effects of taurine on movement ability of mice.Methods: The huckle nerves of the mice were stimulated by electric current at different frequency.The contracting capacity of gastrocnemius and the swimming times of the two groups of mice were recorded.The gastrocnemius was stimulated by a fixed single pulse(2 Hz) and 50% of the maximum contracting amplitude was determined as fatigue.Results: The contracting ability of gastrocnemius of the taurine group increased,compared with the control group,and the time of fatigue and swimming endurance clearly extended(P<0.05).Conclusions: Taurine can strengthen mice's movement ability.
Objective: To observe the effects of taurine on movement ability of mice.Methods: The huckle nerves of the mice were stimulated by electric current at different frequency.The contracting capacity of gastrocnemius and the swimming times of the two groups of mice were recorded.The gastrocnemius was stimulated by a fixed single pulse(2 Hz) and 50% of the maximum contracting amplitude was determined as fatigue.Results: The contracting ability of gastrocnemius of the taurine group increased,compared with the control group,and the time of fatigue and swimming endurance clearly extended(P<0.05).Conclusions: Taurine can strengthen mice's movement ability.
2004, 29(1): 18-20.
Abstract:
Objective: To enhance the awareness of pulmonary complications of acquired immunodeficiency syndrome(AIDS).Methods: Eighteen cases of confirmed AIDS were analyzed.Results: The clinical manifestations of the 18 cases were fever,loss of weight,tiredness,cough and sputum.Among the 18 cases,9 had pneumocystis carinii pneumonia(PCP),7 bacterial infection,6 fungal infection,and 5 tuberculosis.The patients were complicated with skin infection,mouth infection and impairment of many other organs.Conclusions: Pulmonary infection is a major opportunistic infection for AIDS patients at late stage.The most commonly seen were PCP and bacterial infection and then fungal infection and tuberculosis.These patients are often complicated with multi organ-impairments.
Objective: To enhance the awareness of pulmonary complications of acquired immunodeficiency syndrome(AIDS).Methods: Eighteen cases of confirmed AIDS were analyzed.Results: The clinical manifestations of the 18 cases were fever,loss of weight,tiredness,cough and sputum.Among the 18 cases,9 had pneumocystis carinii pneumonia(PCP),7 bacterial infection,6 fungal infection,and 5 tuberculosis.The patients were complicated with skin infection,mouth infection and impairment of many other organs.Conclusions: Pulmonary infection is a major opportunistic infection for AIDS patients at late stage.The most commonly seen were PCP and bacterial infection and then fungal infection and tuberculosis.These patients are often complicated with multi organ-impairments.
2004, 29(1): 21-22.
Abstract:
Objective: To investigate the treatment method for metastatic carcinoma in the axillary lymph node without an obvious primary tumor.Methods: Nineteen female patients who were found to have carcinoma in the axillary lymph node but no obvious primary cancer were analysed.The survival time of the group treated with mastectomy and the group without mastectomy was compared statistical.Results: There was no statistical difference between the survival rate in the two groups(P>0.050).Conclusions: Mastectomy is not necessary for patients with axillary metastatic carcinoma without apparent breast cancer.
Objective: To investigate the treatment method for metastatic carcinoma in the axillary lymph node without an obvious primary tumor.Methods: Nineteen female patients who were found to have carcinoma in the axillary lymph node but no obvious primary cancer were analysed.The survival time of the group treated with mastectomy and the group without mastectomy was compared statistical.Results: There was no statistical difference between the survival rate in the two groups(P>0.050).Conclusions: Mastectomy is not necessary for patients with axillary metastatic carcinoma without apparent breast cancer.
2004, 29(1): 23-24.
Abstract:
Objective: To observe the clinical effect of the paste of hydroxyapatite-iodine-flag as root canal filling material.Methods: The 120 teeth in group A were treated by the paste of hydroxyapatite-iodine-flagy as root canal filling material.The 150 teeth in group B were treated by the paste of zincoxide-eugend and gutta percha point as root canal filling material.The clinical effects were evaluated based on the evidence of X-ray and the follow-up results of 3 months,6 months,1 year and 3 years were compared.Results: The effect was better in group A than in group B(P<0.05);and so was the reconstruction of apical bone(P<0.01 to P<0.005).Conclusions: The paste of hydroxyapatite iodine flag is the ideal material for root canal filling.
Objective: To observe the clinical effect of the paste of hydroxyapatite-iodine-flag as root canal filling material.Methods: The 120 teeth in group A were treated by the paste of hydroxyapatite-iodine-flagy as root canal filling material.The 150 teeth in group B were treated by the paste of zincoxide-eugend and gutta percha point as root canal filling material.The clinical effects were evaluated based on the evidence of X-ray and the follow-up results of 3 months,6 months,1 year and 3 years were compared.Results: The effect was better in group A than in group B(P<0.05);and so was the reconstruction of apical bone(P<0.01 to P<0.005).Conclusions: The paste of hydroxyapatite iodine flag is the ideal material for root canal filling.
2004, 29(1): 25-26.
Abstract:
Objective: To evaluate the clinical effect of direct lacrimal canaliculus intabation and primary canaliculusostomy under microscope for laceration of lower lacrimal canaliculus.Methods: The lacerated paranasal points of 23 patients were found under the microscope and intabated with a silica gel or plastic duct with a piece of silk through it.The two sides were sutured with nylon suture.Three months later,the tube was pulled out.Results: The lacerated points of lacrimal canaliculus in the 23 cases were found successfully at one time and two of them were with the help of a round needle from upper lacrimal canaliculus.During the follow-up of 3 to 6 months,the lacrimal passages were unobstructed in 21 cases;stenosis occurred in one case and obstruction of lacrimal ducts in one case.Conclusions: Direct lacrumal canaliculus intabation and primary canaliculusostomy have the advantages of higher success rate and fewer complications.
Objective: To evaluate the clinical effect of direct lacrimal canaliculus intabation and primary canaliculusostomy under microscope for laceration of lower lacrimal canaliculus.Methods: The lacerated paranasal points of 23 patients were found under the microscope and intabated with a silica gel or plastic duct with a piece of silk through it.The two sides were sutured with nylon suture.Three months later,the tube was pulled out.Results: The lacerated points of lacrimal canaliculus in the 23 cases were found successfully at one time and two of them were with the help of a round needle from upper lacrimal canaliculus.During the follow-up of 3 to 6 months,the lacrimal passages were unobstructed in 21 cases;stenosis occurred in one case and obstruction of lacrimal ducts in one case.Conclusions: Direct lacrumal canaliculus intabation and primary canaliculusostomy have the advantages of higher success rate and fewer complications.
2004, 29(1): 27-28.
Abstract:
Objective: To evaluate the clinical effect of small dose of esmolol on the prevention of cardiovasular side effect after cervical plexus block.Methods: Forty patients undergoing thyroid gland operation were randomly divided into control group and esmolol group.The anaesthesia of cervical plexus block was all performed with "single-puncture".The changes of SBP,DBP,HR and SPO 2 were inspected and recorded before anaesthesia and 3 min,5 min,10 min after anaesthesia.The patients in esmolol group were injected 0.5 mg/kg esmolol immedietly after anaesthesia.Results: The difference of each index between the two groups was not significant before operation(P>0.05).The difference of SBP,DBP and HR was marked in the control group before and during anaesthesia(P<0.01);but in the esmolol group,these indexes were relatively calm,especially those of HR,SBP(P>0.05).The difference was significant after anaesthesia between the two groups(P<0.001).Conclusions: Small dose of esmolol can prevent cardiovasular side-effect after cervical plexus block.
Objective: To evaluate the clinical effect of small dose of esmolol on the prevention of cardiovasular side effect after cervical plexus block.Methods: Forty patients undergoing thyroid gland operation were randomly divided into control group and esmolol group.The anaesthesia of cervical plexus block was all performed with "single-puncture".The changes of SBP,DBP,HR and SPO 2 were inspected and recorded before anaesthesia and 3 min,5 min,10 min after anaesthesia.The patients in esmolol group were injected 0.5 mg/kg esmolol immedietly after anaesthesia.Results: The difference of each index between the two groups was not significant before operation(P>0.05).The difference of SBP,DBP and HR was marked in the control group before and during anaesthesia(P<0.01);but in the esmolol group,these indexes were relatively calm,especially those of HR,SBP(P>0.05).The difference was significant after anaesthesia between the two groups(P<0.001).Conclusions: Small dose of esmolol can prevent cardiovasular side-effect after cervical plexus block.
2004, 29(1): 29-31.
Abstract:
Objective: To investigate the causes of nosocomial infection(NI) in the department of Neurology in the Affiliated Hospital of Bengbu Medical College.Methods: The clinical data of 2 855 patients who were discharged from 1999 to 2002 in this department were reviewed and analyzed.Results: The over-all incidence of NI was 6.09%.The odds between man and female were not obvious.Patients with myeleterosis,hemorrhagic apoplexy,viral encephalitis,peripheral neuropathy or ischemic apoplexy were often affected.The most common NI usually occurred in the lung,upper respiratory tract,gastrointestinal or urinary tract.Pulmonary and the kind of infection were always seen in patients with ischemic apoplexy,while urinary tract infection was always found in patients with myeleterosis.Conclusions: The hospital stay and mortality rate are prolonged because of NI.The occurrence is related to the patients' basic disease, age,invasive manipulation,climate and etc.Prevention and cure of the interior and exterior pathogenic factors should be emphasized to decrease the incidence of NI.
Objective: To investigate the causes of nosocomial infection(NI) in the department of Neurology in the Affiliated Hospital of Bengbu Medical College.Methods: The clinical data of 2 855 patients who were discharged from 1999 to 2002 in this department were reviewed and analyzed.Results: The over-all incidence of NI was 6.09%.The odds between man and female were not obvious.Patients with myeleterosis,hemorrhagic apoplexy,viral encephalitis,peripheral neuropathy or ischemic apoplexy were often affected.The most common NI usually occurred in the lung,upper respiratory tract,gastrointestinal or urinary tract.Pulmonary and the kind of infection were always seen in patients with ischemic apoplexy,while urinary tract infection was always found in patients with myeleterosis.Conclusions: The hospital stay and mortality rate are prolonged because of NI.The occurrence is related to the patients' basic disease, age,invasive manipulation,climate and etc.Prevention and cure of the interior and exterior pathogenic factors should be emphasized to decrease the incidence of NI.
2004, 29(1): 32-33.
Abstract:
Objective: To evaluate video assisted minithoracotomy(VAMT) in the treatment of the subacute pyothorax and the treatment principles.Methods: From August 1999 to July 2002,VAMT was performed in 7 cases of the subacute pyothoraxs.In the course of operation,pus liquid,clot,as well as necrotic tissues were cleared and visceral fiberboard on the lung surface was stripped to make the compressed lung fully expand.As for those with long case history and serious fiberboard adhesion,a "#" cut might be preferred.In this case,the fiberboard on the wall of lung was not stripped in order to prevent blood oozing.Dry gauze could be applied to clean the fiberboard on the thoracic cavity wall so as to remove pus moss.Results: All of the 7 cases recovered well with no severe postoperative complications.Conclusions: VAMT contributes greatly to the eradication of pathogeny,healing of the pus cavity and recovering of lung function.This treatment has proved to be highly effective,and is especially suitable for the patients who are very weak,very young or the patients with a long case history.
Objective: To evaluate video assisted minithoracotomy(VAMT) in the treatment of the subacute pyothorax and the treatment principles.Methods: From August 1999 to July 2002,VAMT was performed in 7 cases of the subacute pyothoraxs.In the course of operation,pus liquid,clot,as well as necrotic tissues were cleared and visceral fiberboard on the lung surface was stripped to make the compressed lung fully expand.As for those with long case history and serious fiberboard adhesion,a "#" cut might be preferred.In this case,the fiberboard on the wall of lung was not stripped in order to prevent blood oozing.Dry gauze could be applied to clean the fiberboard on the thoracic cavity wall so as to remove pus moss.Results: All of the 7 cases recovered well with no severe postoperative complications.Conclusions: VAMT contributes greatly to the eradication of pathogeny,healing of the pus cavity and recovering of lung function.This treatment has proved to be highly effective,and is especially suitable for the patients who are very weak,very young or the patients with a long case history.
2004, 29(1): 34-35.
Abstract:
Objective: To investigate the effect of dose of neostigmine administration on recovery from atracrium induced neuromuscular blockade.Methods: Thirty patients(ASA Ⅰ and Ⅱ) were divided into three groups(each ten),and received neostigmine 20 μg/kg,35 μg/kg and 50 μg/kg,respectively.Results: The speed of recovery from atracurium induced neuromuscular blockade with neostigmine 20 μg/kg was lower as compared with 35 μg/kg and 50 μg/kg,but the difference was not significant between with neostigmine 35 μg/kg and 50 μg/kg.Conclusions: Recovery from atracurium-induced neuromuscular blockade was accelerated when dose of neostigmine was increased from 20 μg/kg to 50 μg/kg,35 μg/kg is the optimal dose of neostigmine for reversal of atracurium induced neuromuscular blockade.
Objective: To investigate the effect of dose of neostigmine administration on recovery from atracrium induced neuromuscular blockade.Methods: Thirty patients(ASA Ⅰ and Ⅱ) were divided into three groups(each ten),and received neostigmine 20 μg/kg,35 μg/kg and 50 μg/kg,respectively.Results: The speed of recovery from atracurium induced neuromuscular blockade with neostigmine 20 μg/kg was lower as compared with 35 μg/kg and 50 μg/kg,but the difference was not significant between with neostigmine 35 μg/kg and 50 μg/kg.Conclusions: Recovery from atracurium-induced neuromuscular blockade was accelerated when dose of neostigmine was increased from 20 μg/kg to 50 μg/kg,35 μg/kg is the optimal dose of neostigmine for reversal of atracurium induced neuromuscular blockade.
2004, 29(1): 36-37.
Abstract:
Objective: To evaluate the influence of propoflo-low dose ketamine in sacral anesthesia in infants on circula,respiratory and bispectral index(BIS).Methods: Thirty infants were randomly divided into two groups(15 cases in each):group A were injected propofol 1.5 mg/kg and maintenanced by continuous propofol 1 mg·kg-1·h-1 infusion;group B were injected propofol 1.5 mg/kg,sedation was maintenanced by continuous propofol 1 mg·kg-1·h-1 and ketamine 0.5 mg·kg-1·h-1 infusion.Sacral anaesthesia was given five minutes after injecting propofol.Rating of propofol infusion was adjusted to BIS in the operation.Results: In group A,HR and MAP during operation were much lower than those before operation(P<0.01).BIS of emergence in group A was lower than that in group B(P<0.05),The emergence time and the dose of propofol during maintenance in group A were much higher than those in group B(P<0.01).Conclusions: Propofol-low dose ketamine has the advantages of hemodynamic stability,lower dose of propofol and rapider emergence as compared with using propofol alone in sacral anaesthesia in infants.
Objective: To evaluate the influence of propoflo-low dose ketamine in sacral anesthesia in infants on circula,respiratory and bispectral index(BIS).Methods: Thirty infants were randomly divided into two groups(15 cases in each):group A were injected propofol 1.5 mg/kg and maintenanced by continuous propofol 1 mg·kg-1·h-1 infusion;group B were injected propofol 1.5 mg/kg,sedation was maintenanced by continuous propofol 1 mg·kg-1·h-1 and ketamine 0.5 mg·kg-1·h-1 infusion.Sacral anaesthesia was given five minutes after injecting propofol.Rating of propofol infusion was adjusted to BIS in the operation.Results: In group A,HR and MAP during operation were much lower than those before operation(P<0.01).BIS of emergence in group A was lower than that in group B(P<0.05),The emergence time and the dose of propofol during maintenance in group A were much higher than those in group B(P<0.01).Conclusions: Propofol-low dose ketamine has the advantages of hemodynamic stability,lower dose of propofol and rapider emergence as compared with using propofol alone in sacral anaesthesia in infants.
2004, 29(1): 38-39.
Abstract:
Objective: To evaluate the efficacy and toxicity of home made vinorelbine plus cisplatin therapy in the treatment of advanced non-small cell lung cancer(NSCLC).Methods: Twenty-five cases of stage Ⅲb to Ⅳ NSCLC received vinorelbine and cisplatin therapy.One course of treatment consisted of 3 to 4 weeks and the clinical effects were assessed after two courses of treatment.Results: The overall response rate was 44%.The medium response time was 3.7 months,and the medium survival time 9.8 months.The most frequent toxic reactions were leukopenia(96%) and phlebitis(44%).Conclusions: Vinorellbine plus cisplatin chemotherapy has a good curative effect and low toxicity in treatment of advanced NSCLC.
Objective: To evaluate the efficacy and toxicity of home made vinorelbine plus cisplatin therapy in the treatment of advanced non-small cell lung cancer(NSCLC).Methods: Twenty-five cases of stage Ⅲb to Ⅳ NSCLC received vinorelbine and cisplatin therapy.One course of treatment consisted of 3 to 4 weeks and the clinical effects were assessed after two courses of treatment.Results: The overall response rate was 44%.The medium response time was 3.7 months,and the medium survival time 9.8 months.The most frequent toxic reactions were leukopenia(96%) and phlebitis(44%).Conclusions: Vinorellbine plus cisplatin chemotherapy has a good curative effect and low toxicity in treatment of advanced NSCLC.
2004, 29(1): 40-42.
Abstract:
Objective: To treat obstructive azoospermia by percutaneous epididymal,testicular sperm aspiration(PETSA) and intracytoplasmic sperm injection(ICSI).Methods: Eight couples were performed PETSA and ICSI.The male were treated by PETSA to obtain normal spermatozoon.Ovarian stimulation was achieved by the long or short protocol.All metaphas Ⅱ (MⅡ) oocytes were selected for ICSI.Results: Forty-six MⅡ oocytes were injected in 9 cycles;33 fertilized;and 22 embryos were implanted.Three couples achieved clinical pregnancy.Conclusions: PETSA and ICSI are effective approaches in treatment of obstructive azoospermia.
Objective: To treat obstructive azoospermia by percutaneous epididymal,testicular sperm aspiration(PETSA) and intracytoplasmic sperm injection(ICSI).Methods: Eight couples were performed PETSA and ICSI.The male were treated by PETSA to obtain normal spermatozoon.Ovarian stimulation was achieved by the long or short protocol.All metaphas Ⅱ (MⅡ) oocytes were selected for ICSI.Results: Forty-six MⅡ oocytes were injected in 9 cycles;33 fertilized;and 22 embryos were implanted.Three couples achieved clinical pregnancy.Conclusions: PETSA and ICSI are effective approaches in treatment of obstructive azoospermia.
2004, 29(1): 43-44.
Abstract:
Objective: To study the factors leading to successful operation for intermittent exotropia and postoperative visual function recovery.Methods: The data of 106 cases of intermittent exotropia who had received operation and followed up for six weeks to one year were analysed.Results: The rates of right position were 84.91%(one week after operation),76.42%(six weeks after operation) and 75.47%(one year after operation).Fusional faculty before operation was essential for the recovery of eyesight function.The younger the patients were,the better the recovery would achieve(P<0.005).Conclusions: Patients with intermittent exotropia should resort to surgical treatment as early as possible.
Objective: To study the factors leading to successful operation for intermittent exotropia and postoperative visual function recovery.Methods: The data of 106 cases of intermittent exotropia who had received operation and followed up for six weeks to one year were analysed.Results: The rates of right position were 84.91%(one week after operation),76.42%(six weeks after operation) and 75.47%(one year after operation).Fusional faculty before operation was essential for the recovery of eyesight function.The younger the patients were,the better the recovery would achieve(P<0.005).Conclusions: Patients with intermittent exotropia should resort to surgical treatment as early as possible.
2004, 29(1): 45-47.
Abstract:
Objective: To summarize the experience in treating 8 cases of hypertensive cerebral hemorrhage with the aid of neuroendoscopy.Methods: The hematoma was evacucated with the aid of nerroendoscopy,then the hematoma cavitary drainage and comprehensive remedy were performed.Results: Four patients survived,2 were seriously disabled and 2 died.Conclusions: Careful selection of operative indications can decrease the death rate and improve the curative effect for the patients with hypertensive cerebral hemorrahage.
Objective: To summarize the experience in treating 8 cases of hypertensive cerebral hemorrhage with the aid of neuroendoscopy.Methods: The hematoma was evacucated with the aid of nerroendoscopy,then the hematoma cavitary drainage and comprehensive remedy were performed.Results: Four patients survived,2 were seriously disabled and 2 died.Conclusions: Careful selection of operative indications can decrease the death rate and improve the curative effect for the patients with hypertensive cerebral hemorrahage.
2004, 29(1): 65-66.
Abstract:
Objective: To explore the relationship between lupus nephritis(LN) and the anticardiolipin antibodies(ACA).Methods: The ACA in 190 patients with light or severe damage in the kidney and 90 healthy volunteers was detected and compared.Results: The ACA positive rate in patients with LN was higher(81.6%) than that in the healthy controls(1.1%).The ACA positive rate in the patients with severe damage in the kidney was higher(92.0%) than that in the patients with light damage in the kidney(70.0%)(P<0.01).The difference was significant(P<0.01).Conclusions: The examination of ACA provides an important clue to clinical diagnosis of LN.ACA is an index in supervising the state of LN patients.It is of some value for clinical treatment of LN. Back
Objective: To explore the relationship between lupus nephritis(LN) and the anticardiolipin antibodies(ACA).Methods: The ACA in 190 patients with light or severe damage in the kidney and 90 healthy volunteers was detected and compared.Results: The ACA positive rate in patients with LN was higher(81.6%) than that in the healthy controls(1.1%).The ACA positive rate in the patients with severe damage in the kidney was higher(92.0%) than that in the patients with light damage in the kidney(70.0%)(P<0.01).The difference was significant(P<0.01).Conclusions: The examination of ACA provides an important clue to clinical diagnosis of LN.ACA is an index in supervising the state of LN patients.It is of some value for clinical treatment of LN. Back
2004, 29(1): 67-68.
Abstract:
Objective: To explore the role of the real time fluorescence quantitative polymerase chain reaction (FQ PCR) in detecting heptitis B virus (HBV) DNA.Methods: The sera from 196 patients were measured by FQ PCR.Results: In the 112 HBeAg (+)/HBeAb (-) samples,the FQ PCR results were all positive,with 1.08×107/ml of HBV DNA on the average.In the 68 HBeAg (-)/HBeAb (+) samples,the positive rate was 66.2% with a duplication amount of 6.12×105/ml on the average.In the 16 HBeAg (-)/HBeAb (-) samples,the duplication amount was 2.72×104/ml.Conclusions: FQ PCR can ensure the accurate quantity.It is a useful method in understanding HBV duplication and evaluating the therapeutic effect.
Objective: To explore the role of the real time fluorescence quantitative polymerase chain reaction (FQ PCR) in detecting heptitis B virus (HBV) DNA.Methods: The sera from 196 patients were measured by FQ PCR.Results: In the 112 HBeAg (+)/HBeAb (-) samples,the FQ PCR results were all positive,with 1.08×107/ml of HBV DNA on the average.In the 68 HBeAg (-)/HBeAb (+) samples,the positive rate was 66.2% with a duplication amount of 6.12×105/ml on the average.In the 16 HBeAg (-)/HBeAb (-) samples,the duplication amount was 2.72×104/ml.Conclusions: FQ PCR can ensure the accurate quantity.It is a useful method in understanding HBV duplication and evaluating the therapeutic effect.
2004, 29(1): 69-71.
Abstract:
Objective: To evaluate the function of the CELL DYN1700 automated hematologic analyser in clinical application.Methods: The accuracy,precision,contamination carrying rate and linearity range were measured using clinical samples.Results: The CV with in the group were as follows:white blood cell(WBC) 1.9%,red blood cell(RBC) 1.65%,hemoglobin(Hb) 1.35%,hematocrit(HCT) 2.0%,mean corpuscular volume(MCV) 0.93% and blood platelet counts(BPC) 3.2%.The CV between the groups were as follows: in the high blood value group,WBC 2.7%,RBC 2.21%,Hb 1.86%,HCT 2.8%,MCV 1.9% and BPC 6.0%;in the normal blood value group,WBC 2.1%,RBC 1.9%,Hb 1.7%,HCT 2.3%,MCV1.8% and BPC 3.6%;in the low blood value group,WBC 2.0%,RBC 2.1%,Hb 1.68%,HCT 2.4%,MCV 1.92% and BPC 6.8%.The lineariyt range was wide:WBC(0.5 to 90.0)×109/L,RBC(1.0 to 7.0)×1012/L,Hb(25.0 to 200.0)g/L、BPC(10.0 to 999.0)×109/L.The contaimination carrying rate was low:WBC 0.1%,RBC 0%,Hb 0%,BPC 0.5%.The accuracy of this analyzer was high.The results showed no significant difference between the EDTA-K2 anti freezing vessl blood analyser and conventional detection.Conclusions: This analyzer has the advantages of high accuracy,high repeatability,easy operation,fast detection and little cross pollution.It can test 18 pharaments with vessl blood or finger blood.
Objective: To evaluate the function of the CELL DYN1700 automated hematologic analyser in clinical application.Methods: The accuracy,precision,contamination carrying rate and linearity range were measured using clinical samples.Results: The CV with in the group were as follows:white blood cell(WBC) 1.9%,red blood cell(RBC) 1.65%,hemoglobin(Hb) 1.35%,hematocrit(HCT) 2.0%,mean corpuscular volume(MCV) 0.93% and blood platelet counts(BPC) 3.2%.The CV between the groups were as follows: in the high blood value group,WBC 2.7%,RBC 2.21%,Hb 1.86%,HCT 2.8%,MCV 1.9% and BPC 6.0%;in the normal blood value group,WBC 2.1%,RBC 1.9%,Hb 1.7%,HCT 2.3%,MCV1.8% and BPC 3.6%;in the low blood value group,WBC 2.0%,RBC 2.1%,Hb 1.68%,HCT 2.4%,MCV 1.92% and BPC 6.8%.The lineariyt range was wide:WBC(0.5 to 90.0)×109/L,RBC(1.0 to 7.0)×1012/L,Hb(25.0 to 200.0)g/L、BPC(10.0 to 999.0)×109/L.The contaimination carrying rate was low:WBC 0.1%,RBC 0%,Hb 0%,BPC 0.5%.The accuracy of this analyzer was high.The results showed no significant difference between the EDTA-K2 anti freezing vessl blood analyser and conventional detection.Conclusions: This analyzer has the advantages of high accuracy,high repeatability,easy operation,fast detection and little cross pollution.It can test 18 pharaments with vessl blood or finger blood.
2004, 29(1): 71-73.
Abstract:
Objective: To compare the reliability of microscopic examination and urine dipstick evaluation with the urine culture in diagnosing urinary tract infection(UTI) in children.Methods: Fresh midstream clean-catch urine samples were collected in accordiance with standard guideline,10 μl of the urine specimen was used for bacteria culture(colony counts of ≥ 105 CFU/ml),the other portion was used for test with reagent strip for presence of LE,NIT and microscopic examination.Results: One hundred and nine samples were tested in all,23 patients(21.1%) had bacteria cultures of 100 000 or more colony-forming units of a uropathogen per milliliter.The sensitivities of the microscopic examination and dipstick test in identifying patients with positive cultures were 73.9% and 82.6%,and the specificities were 95.3% and 88.4%,respectively.Four patients(17.4%) had negative dipstick screening tests but positive urine cultures,and other four patients had positive microscopic examination but negative urine cultures.The positive predict value(PPV) of urine microscopic examination and dipstick evaluation was 81.0% and 65.5% respectively.Conclusions: Urine reagent strips tests and microscopic examination are not sufficiently sensitive in the detection of UTI;therefore,bacteria culture must be adopted.
Objective: To compare the reliability of microscopic examination and urine dipstick evaluation with the urine culture in diagnosing urinary tract infection(UTI) in children.Methods: Fresh midstream clean-catch urine samples were collected in accordiance with standard guideline,10 μl of the urine specimen was used for bacteria culture(colony counts of ≥ 105 CFU/ml),the other portion was used for test with reagent strip for presence of LE,NIT and microscopic examination.Results: One hundred and nine samples were tested in all,23 patients(21.1%) had bacteria cultures of 100 000 or more colony-forming units of a uropathogen per milliliter.The sensitivities of the microscopic examination and dipstick test in identifying patients with positive cultures were 73.9% and 82.6%,and the specificities were 95.3% and 88.4%,respectively.Four patients(17.4%) had negative dipstick screening tests but positive urine cultures,and other four patients had positive microscopic examination but negative urine cultures.The positive predict value(PPV) of urine microscopic examination and dipstick evaluation was 81.0% and 65.5% respectively.Conclusions: Urine reagent strips tests and microscopic examination are not sufficiently sensitive in the detection of UTI;therefore,bacteria culture must be adopted.
2004, 29(1): 74-76.
Abstract:
Objective: To study the value of 12 tumor markers in protein chip system for multi-tumor detection(C12) in the diagnosis of the primary liver cancer.Methods: The system of C12 was used to quantitatively detect the 12 tumor markers(CA125,Ferritin,CEA,AFP,CA19-9,CA15-3,NSE,CA242,PSA,Free PSA,HGH,β-hCG) in the sera of 128 patients with primary liver cancer,23 patients with hepatocirrhosis,and 98 healthy controls.Results: The quantitative detection proved positive in 112 of the 128 patients with primary liver cancer;the sensitivity was raised from 58.6% by AFP single index detection to 87.5% and the specificity reached 83.5%.In addition,Ferritin positive rate achieved 41.4%,and in the sera of some liver cancer patients,the difference of β-hCG,NSE,PSA,HGH,CA19-9,CA125,CEA,AFP,CA15-3 and CA242 items was statistically significant,compared with the contrast group and hepatocirrhosis group(P<0.01).Conclusions: The allied detection is more effective than the traditional single index detection.
Objective: To study the value of 12 tumor markers in protein chip system for multi-tumor detection(C12) in the diagnosis of the primary liver cancer.Methods: The system of C12 was used to quantitatively detect the 12 tumor markers(CA125,Ferritin,CEA,AFP,CA19-9,CA15-3,NSE,CA242,PSA,Free PSA,HGH,β-hCG) in the sera of 128 patients with primary liver cancer,23 patients with hepatocirrhosis,and 98 healthy controls.Results: The quantitative detection proved positive in 112 of the 128 patients with primary liver cancer;the sensitivity was raised from 58.6% by AFP single index detection to 87.5% and the specificity reached 83.5%.In addition,Ferritin positive rate achieved 41.4%,and in the sera of some liver cancer patients,the difference of β-hCG,NSE,PSA,HGH,CA19-9,CA125,CEA,AFP,CA15-3 and CA242 items was statistically significant,compared with the contrast group and hepatocirrhosis group(P<0.01).Conclusions: The allied detection is more effective than the traditional single index detection.
2004, 29(1): 77-79.
Abstract:
Objective: To investigate the relativity between Pre-S1 antigen and hepatitis B virus serum markers(HBV M).Methods: Four hundred and fifty-seven HBsAg positive serums were inspected by ELISA and the relationship between HBV-M and Pre-S1 antigen were analyzed.Results: Pre-S1 antigen and HBV-M had different relativity and independent inspection value.Conclusions: As the index of infection and reproduction of HBV,Pre-S1 antigen is much more sensitive than HBeAg.Pre-S1 antigen is related to the activity of HBV and has the supplemental function to HBV-M inspection.
Objective: To investigate the relativity between Pre-S1 antigen and hepatitis B virus serum markers(HBV M).Methods: Four hundred and fifty-seven HBsAg positive serums were inspected by ELISA and the relationship between HBV-M and Pre-S1 antigen were analyzed.Results: Pre-S1 antigen and HBV-M had different relativity and independent inspection value.Conclusions: As the index of infection and reproduction of HBV,Pre-S1 antigen is much more sensitive than HBeAg.Pre-S1 antigen is related to the activity of HBV and has the supplemental function to HBV-M inspection.
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