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2004 Vol. 29, No. 2
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2004, 29(2): 95-97.
Abstract:
Objective: To explore the effects of asparagus extract on the inhibition of proliferation and apoptosis in human melanoma A375 cells.Methods: The cell proliferation inhibition was measured by methyl thiazolyl tetrazolium(MTT) colorimetric assay;apoptosis and necrosis in A375 cells were analysed by flow cytometry.Results: There was obviously a concentration-dependent relationship between the asparagus extract and the inhibition of A375 cells proliferation(P<0.01).The rates of apoptosis and necrosis in A375 cells were 2.39%, 8.85%,8.71%,5.16% and 1.65%,1.51%,22.10%,82.80% respectively as treated at doses of asparagus extract 200 mg/ml,400 mg/ml,600 mg/ml and 800 mg/ml.Conclusions: Asparagus extract can obviously inhibit the proliferation of A375 cells.There exists a concentration dependent relationship between asparagus extract and the total rates of apoptosis and necrosis.
Objective: To explore the effects of asparagus extract on the inhibition of proliferation and apoptosis in human melanoma A375 cells.Methods: The cell proliferation inhibition was measured by methyl thiazolyl tetrazolium(MTT) colorimetric assay;apoptosis and necrosis in A375 cells were analysed by flow cytometry.Results: There was obviously a concentration-dependent relationship between the asparagus extract and the inhibition of A375 cells proliferation(P<0.01).The rates of apoptosis and necrosis in A375 cells were 2.39%, 8.85%,8.71%,5.16% and 1.65%,1.51%,22.10%,82.80% respectively as treated at doses of asparagus extract 200 mg/ml,400 mg/ml,600 mg/ml and 800 mg/ml.Conclusions: Asparagus extract can obviously inhibit the proliferation of A375 cells.There exists a concentration dependent relationship between asparagus extract and the total rates of apoptosis and necrosis.
2004, 29(2): 97-98.
Abstract:
Objective: To observe the process that eggs of Taenia solium were hatched using sodium hypochrite.Methods: The eggs of Taenia solium were hatched with 1.0% sodium hypochrite solution and the process was observed by microscope.Results: The embryo membrane of Taenia solium eggs dissolved and oncospheres transgressed.Conclusions: The essence of hatching Taenia solium eggs by sodium hypochrite method is that the embryo membrane of Taenia solium eggs is dissolved.
Objective: To observe the process that eggs of Taenia solium were hatched using sodium hypochrite.Methods: The eggs of Taenia solium were hatched with 1.0% sodium hypochrite solution and the process was observed by microscope.Results: The embryo membrane of Taenia solium eggs dissolved and oncospheres transgressed.Conclusions: The essence of hatching Taenia solium eggs by sodium hypochrite method is that the embryo membrane of Taenia solium eggs is dissolved.
2004, 29(2): 99-101.
Abstract:
Objective: To develop and identify the monoclonal antibody(McAb) against cysticercus cellulosae KD26 antigen,so as to provide useful help and further study.Methods: BALB/C mice were immunized 3 times with KD26 antigen of cysticercus cellulosae by SDS-PAGE(mixed with Freund's incomplet adjuvant),and the spleen cells of immunized mice were fused with myeloma cell line SP2/0(in the ratio of 10:1) in the HAT medium containing 50% PEG(4 000 MW),then incubated,selected and cloned.Numerical analysis on chromosome was performed by using metephase technique.The type and titer of the McAb was assayed by agar double diffusion technique and ELISA methods.Results: After being selected and cloned 3 times by using routine limited dilution method,one hybridoma cell line XH was generated,which contained 96 chromosomes and could steadily secrete specific McAb.The McAb belonged to IgG1 type.The titer was 1:1 000.The McAb had no cross reactions with the antigens of Schistosome japonicum,Toxoplasma gondii and Echinococcus granulosus.Conclusions: By the hybridoma technique,we have established the hybridoma cell line which can secrete specific and high titers McAb against cysticercus cellulosae KD26 antigen.
Objective: To develop and identify the monoclonal antibody(McAb) against cysticercus cellulosae KD26 antigen,so as to provide useful help and further study.Methods: BALB/C mice were immunized 3 times with KD26 antigen of cysticercus cellulosae by SDS-PAGE(mixed with Freund's incomplet adjuvant),and the spleen cells of immunized mice were fused with myeloma cell line SP2/0(in the ratio of 10:1) in the HAT medium containing 50% PEG(4 000 MW),then incubated,selected and cloned.Numerical analysis on chromosome was performed by using metephase technique.The type and titer of the McAb was assayed by agar double diffusion technique and ELISA methods.Results: After being selected and cloned 3 times by using routine limited dilution method,one hybridoma cell line XH was generated,which contained 96 chromosomes and could steadily secrete specific McAb.The McAb belonged to IgG1 type.The titer was 1:1 000.The McAb had no cross reactions with the antigens of Schistosome japonicum,Toxoplasma gondii and Echinococcus granulosus.Conclusions: By the hybridoma technique,we have established the hybridoma cell line which can secrete specific and high titers McAb against cysticercus cellulosae KD26 antigen.
2004, 29(2): 101-103.
Abstract:
Objective: To investigate the success rate and reliability of creating global cerebral ischemia reperfusion injury model by obstructing the bilateral common carolid arteries and pressing the cervix and ventilating the mice in the artificial way and so on.Methods: Artificial respiration was performed using rodent micro-ventilator during the whole experiment course.ECG and EEG were detected carefully.EEG turnning to a straight line was used as the criterion of full cerebral ischemia.Pathological examination was performed in the cerebral cortex and hippocampe.Lactate dehydrogenase (LDH) and creatine phosphokinase(CPK) were determined in the cerebral cortex.Results: EEG turned to a straight line after a few seconds of ischemia and didn't recover within 30 minutes of ishemic and 60 minutes of reperfusion.The LDH and CPK activity of ischemia and reperfusion group was significantly lower than that of control group(P<0.01).CPK activity of reperfusion group was significantly lower than that of ischemia group(P<0.01).Pathologic examination showed that after 30 minutes of ischemia,the cells of cerebral cortex and hippocampe pyramid pyknosed,a small number of neurons degenerated and swelled.The above mentioned damage worsened after 60 minutes of reperfusion.Conclusions: This improved operative method is simple and practical.The global cerebral ischemia-reperfusion injury is obvious in the model,with higher success rate and lower mortality.
Objective: To investigate the success rate and reliability of creating global cerebral ischemia reperfusion injury model by obstructing the bilateral common carolid arteries and pressing the cervix and ventilating the mice in the artificial way and so on.Methods: Artificial respiration was performed using rodent micro-ventilator during the whole experiment course.ECG and EEG were detected carefully.EEG turnning to a straight line was used as the criterion of full cerebral ischemia.Pathological examination was performed in the cerebral cortex and hippocampe.Lactate dehydrogenase (LDH) and creatine phosphokinase(CPK) were determined in the cerebral cortex.Results: EEG turned to a straight line after a few seconds of ischemia and didn't recover within 30 minutes of ishemic and 60 minutes of reperfusion.The LDH and CPK activity of ischemia and reperfusion group was significantly lower than that of control group(P<0.01).CPK activity of reperfusion group was significantly lower than that of ischemia group(P<0.01).Pathologic examination showed that after 30 minutes of ischemia,the cells of cerebral cortex and hippocampe pyramid pyknosed,a small number of neurons degenerated and swelled.The above mentioned damage worsened after 60 minutes of reperfusion.Conclusions: This improved operative method is simple and practical.The global cerebral ischemia-reperfusion injury is obvious in the model,with higher success rate and lower mortality.
2004, 29(2): 104-105.
Abstract:
Objective: To observe the effect of neurotrophin-3(NT-3) gene transfer on the function recovery of facial muscle denervation in rats.Methods: The model of facial nerve lesion was established in rats by cutting the marginal mandibular branch and buccal branches of facial nerve.The recombinant plasmid pcDNA3.1(+)-NT-3 and DC-chol liposome admixture were injected into the muscles around the injured facial nerve.RT-PCR was adopted to confirm whether NT-3 cDNA expressed after the injection,and the movement of the rats'palp was observed.Results: The muscles were infected by injecting the DC-chol mediated NT-3 transfer and NT-3 gene was expressed.The movement of the rats'palp at the side injected NT-3 began to recover earlier than the control side.Conclusions: Transfer of NT-3 can promote the recovery of the function of the injured facial nerve by increasing local expression of exogenous NT-3.
Objective: To observe the effect of neurotrophin-3(NT-3) gene transfer on the function recovery of facial muscle denervation in rats.Methods: The model of facial nerve lesion was established in rats by cutting the marginal mandibular branch and buccal branches of facial nerve.The recombinant plasmid pcDNA3.1(+)-NT-3 and DC-chol liposome admixture were injected into the muscles around the injured facial nerve.RT-PCR was adopted to confirm whether NT-3 cDNA expressed after the injection,and the movement of the rats'palp was observed.Results: The muscles were infected by injecting the DC-chol mediated NT-3 transfer and NT-3 gene was expressed.The movement of the rats'palp at the side injected NT-3 began to recover earlier than the control side.Conclusions: Transfer of NT-3 can promote the recovery of the function of the injured facial nerve by increasing local expression of exogenous NT-3.
2004, 29(2): 106-108.
Abstract:
Objective: To compare three methods of detecting Toxoplasma-gondii-infected-cells in culture and to explore a better one for pathogenic diagnosis of toxoplasmosis.Methods: The Toxoplasma tachyzoites were added in the Hela cell and THP-1 cell culture system,then the infected cells were counted at different times(3 to 9 hours) by light microscope with the Giemsa staining,by fluorescence microscope with IFA,and by flow cytometry(FCM).Results: The infection rates of Hela cells examined by Giemsa staining and by IFA were 22.0‰ and 24.3‰ respectively,and the infection rate of THP-1 cells examined by FCM was 42.0‰(P<0.05). Conclusions: The detection rate of affected THP-1 cells is the highest by FCM,while it is similar by IFA or Giemsa staining.
Objective: To compare three methods of detecting Toxoplasma-gondii-infected-cells in culture and to explore a better one for pathogenic diagnosis of toxoplasmosis.Methods: The Toxoplasma tachyzoites were added in the Hela cell and THP-1 cell culture system,then the infected cells were counted at different times(3 to 9 hours) by light microscope with the Giemsa staining,by fluorescence microscope with IFA,and by flow cytometry(FCM).Results: The infection rates of Hela cells examined by Giemsa staining and by IFA were 22.0‰ and 24.3‰ respectively,and the infection rate of THP-1 cells examined by FCM was 42.0‰(P<0.05). Conclusions: The detection rate of affected THP-1 cells is the highest by FCM,while it is similar by IFA or Giemsa staining.
2004, 29(2): 108-112.
Abstract:
Objective: To investigate the processing of the artery smooth muscle cells patch and to observe the properties of large-conductance calcium-activated potassium channels in them.Methods: The humans mesenteric artery was dissociated from human mesenteriolum,and single smooth muscle cells were isolated by enzyme and put into symmetric high potassium solution.The channel current was recorded by using patch clamp and the data were analyzed by the computer.The processing of three kinds of ptches of artery smooth muscle cell and the characteristics of BKCa were observed.Results: In the experiment,cell attached,inside-out and whole cell recording patches were produced respectively on the cytomembrane of mesenteric artery smooth muscle.BKCa channels of smooth muscle cells isolated by enzyme were sensitive to[Ca2+]i and dependant on voltage;the channels opened more frequently with the increase of free[Ca2+]i and membrane potential.The conductance of enzymatic smooth muscle cells in this experiments was 198.6±38.09 pS;The channels were sensitive to TEA+:when 50 mmol/L TEA+ was used,these channels were entirely blocked.Conclusions: The experiments suggested that BKCa channels in smooth muscle cells isolated by enzyme were sensitive to Ca2+,dependent of voltage sensitive to TEA+ and had the properties of large conductance.
Objective: To investigate the processing of the artery smooth muscle cells patch and to observe the properties of large-conductance calcium-activated potassium channels in them.Methods: The humans mesenteric artery was dissociated from human mesenteriolum,and single smooth muscle cells were isolated by enzyme and put into symmetric high potassium solution.The channel current was recorded by using patch clamp and the data were analyzed by the computer.The processing of three kinds of ptches of artery smooth muscle cell and the characteristics of BKCa were observed.Results: In the experiment,cell attached,inside-out and whole cell recording patches were produced respectively on the cytomembrane of mesenteric artery smooth muscle.BKCa channels of smooth muscle cells isolated by enzyme were sensitive to[Ca2+]i and dependant on voltage;the channels opened more frequently with the increase of free[Ca2+]i and membrane potential.The conductance of enzymatic smooth muscle cells in this experiments was 198.6±38.09 pS;The channels were sensitive to TEA+:when 50 mmol/L TEA+ was used,these channels were entirely blocked.Conclusions: The experiments suggested that BKCa channels in smooth muscle cells isolated by enzyme were sensitive to Ca2+,dependent of voltage sensitive to TEA+ and had the properties of large conductance.
2004, 29(2): 113-115.
Abstract:
Objective: To observe the influence of soybean isoflavone(SI) on the fat level and lipid peroxides in rats.Methods: Fifty wistar rats were randomly divided into five groups and fed on foundation feed high fat feed,and high fat feed mixed with 30 mg/kg,90 mg/kg and 270 mg/kg soybean isoflavone respectively.The experiment lasted for 6 weeks.Results: SI could obviously inhibit the rise of low density lipoprotein(LDL) induced by high fat feed in the plasma(P<0.01).SI had preventive effect on the rise of lipid peroxide induced by feed and it improved the activity of GSH px,SOD and CAT(P<0.05 to P<0.01).Conclusions: SI can decrease the hypertriglyceride,improve the abnormal oxidization caused by high fat feed,and alleviate the lesion due to peroxidation.
Objective: To observe the influence of soybean isoflavone(SI) on the fat level and lipid peroxides in rats.Methods: Fifty wistar rats were randomly divided into five groups and fed on foundation feed high fat feed,and high fat feed mixed with 30 mg/kg,90 mg/kg and 270 mg/kg soybean isoflavone respectively.The experiment lasted for 6 weeks.Results: SI could obviously inhibit the rise of low density lipoprotein(LDL) induced by high fat feed in the plasma(P<0.01).SI had preventive effect on the rise of lipid peroxide induced by feed and it improved the activity of GSH px,SOD and CAT(P<0.05 to P<0.01).Conclusions: SI can decrease the hypertriglyceride,improve the abnormal oxidization caused by high fat feed,and alleviate the lesion due to peroxidation.
2004, 29(2): 115-117.
Abstract:
Objective: To investigate whether the combination of thermotherapy and HCPT has a synergic effect on the hepatoma cells(SMMC-7721 cell line) in vitro.Methods: The experiment consists of four groups:thermotherapy,chemotherapy(HCPT),thermo-chemotherapy(chermotherapy combined with HCPT) and control group.Results: Thermotherapy within 30 minutes with a temperature of 43℃,44.5℃ had an obviously inhibited SMMC-7721 cells(P<0.01),while 40℃,41.5℃ hardly had any effect on the cells(P>0.05).HCPT had a great cytotoxicity effect on SMMC-7721 cells in dose and time-dependant manner(P<0.01).The cytotoxicity could be enhanced when HCPT was combined with thermotherapy(P<0.01).Conclusions: The combination of HCPT and themotherapy has a remarkable synergic effect on hepatoma cells.
Objective: To investigate whether the combination of thermotherapy and HCPT has a synergic effect on the hepatoma cells(SMMC-7721 cell line) in vitro.Methods: The experiment consists of four groups:thermotherapy,chemotherapy(HCPT),thermo-chemotherapy(chermotherapy combined with HCPT) and control group.Results: Thermotherapy within 30 minutes with a temperature of 43℃,44.5℃ had an obviously inhibited SMMC-7721 cells(P<0.01),while 40℃,41.5℃ hardly had any effect on the cells(P>0.05).HCPT had a great cytotoxicity effect on SMMC-7721 cells in dose and time-dependant manner(P<0.01).The cytotoxicity could be enhanced when HCPT was combined with thermotherapy(P<0.01).Conclusions: The combination of HCPT and themotherapy has a remarkable synergic effect on hepatoma cells.
2004, 29(2): 118-120.
Abstract:
Objective: To make comparison among 4 methods for detecting L-form of Mycobacterium tuberculosis and to study its clinical significance in patients with multidrug resistant pulmonary tuberculosis.Methods: Acid-fast sputum smear,modified L J culture,L-form culture and PCR technique were conducted in 103 cases of multidrug resistant pulmonary tuberculosis(MDR PTB).Results: The positive rate of L-form culture was higher than that of acid-fast sputum smear,modified L-J culture,and PCR(P<0.01 to P<0.005).Conclusions: Sputum culture for L-form of Mycobacterium tuberculosis can raise the detected positive rate and facilitate early rapid diagnosis.The infectious rate among patients with MDR PTB is much high,which simultaneously is an important cause of recurrence and aggravation of tuberculosis.
Objective: To make comparison among 4 methods for detecting L-form of Mycobacterium tuberculosis and to study its clinical significance in patients with multidrug resistant pulmonary tuberculosis.Methods: Acid-fast sputum smear,modified L J culture,L-form culture and PCR technique were conducted in 103 cases of multidrug resistant pulmonary tuberculosis(MDR PTB).Results: The positive rate of L-form culture was higher than that of acid-fast sputum smear,modified L-J culture,and PCR(P<0.01 to P<0.005).Conclusions: Sputum culture for L-form of Mycobacterium tuberculosis can raise the detected positive rate and facilitate early rapid diagnosis.The infectious rate among patients with MDR PTB is much high,which simultaneously is an important cause of recurrence and aggravation of tuberculosis.
2004, 29(2): 121-122.
Abstract:
Objective: To investigate the diagnosis and treatment of cardiac myxomas.Methods: Twelve cases of cardiac myxoma were diagnosed by the case history,symptoms and auxiliary,examinations including electrocardiogram and echocardiography.All the tumors were removed by moderate hypothermic cardiopulmonary bypass.Results: There was no operative or peri-operative death.The patients didn't show signs of recurrence or metastasis during the follow-up of 1 month to 6 years after operation.Conclusions: Echocardiography plays an important role in the diagnosis of cardiac myxomas.Once the diagnosis is confirmed,the patients should resort to surgical treatment as soon as possible.
Objective: To investigate the diagnosis and treatment of cardiac myxomas.Methods: Twelve cases of cardiac myxoma were diagnosed by the case history,symptoms and auxiliary,examinations including electrocardiogram and echocardiography.All the tumors were removed by moderate hypothermic cardiopulmonary bypass.Results: There was no operative or peri-operative death.The patients didn't show signs of recurrence or metastasis during the follow-up of 1 month to 6 years after operation.Conclusions: Echocardiography plays an important role in the diagnosis of cardiac myxomas.Once the diagnosis is confirmed,the patients should resort to surgical treatment as soon as possible.
2004, 29(2): 123-125.
Abstract:
Objective: To investigate the relationship between the development of gastric cancer and different subtypes and local patterns of intestinal metaplasia.Methods: Histopathological and histochemical methods were used to assess the intestinal metaplasia of tissues from 68 cases of gastric carcinomas and 67 cases of gastritis as control by gastroscopal biopsy.The relationship between gastric cancer and different local patterns of intestinal metaplasia was analyzed.Results: Four kinds of intestinal metaplasia were observed:focal pattern,pylorus-dominant pattern,body of stomach pattern and diffuse pattern.Conclusions: Incomplete intestinal metaplasia mainly develop in the body of stomach pattern and diffuse pattern.In the incomplete intestinal metaplasia occurring along the lesser curvature,the incidence of gascric cancer in different patterns varies.
Objective: To investigate the relationship between the development of gastric cancer and different subtypes and local patterns of intestinal metaplasia.Methods: Histopathological and histochemical methods were used to assess the intestinal metaplasia of tissues from 68 cases of gastric carcinomas and 67 cases of gastritis as control by gastroscopal biopsy.The relationship between gastric cancer and different local patterns of intestinal metaplasia was analyzed.Results: Four kinds of intestinal metaplasia were observed:focal pattern,pylorus-dominant pattern,body of stomach pattern and diffuse pattern.Conclusions: Incomplete intestinal metaplasia mainly develop in the body of stomach pattern and diffuse pattern.In the incomplete intestinal metaplasia occurring along the lesser curvature,the incidence of gascric cancer in different patterns varies.
2004, 29(2): 125-127.
Abstract:
Objective: To clarify the features of colorectal carcinoma in patients over the age of 70 years.Methods: The clinical data of 62 patients(over 70 years of age) admitted from 2000 to 2002 were analyzed retrospectively.Results: Of the 62 cases,the incidence of Dukes C or D stage accounted for 62.9%(39/62),acute obstructing colorectal carcinoma 29.8%(18/62) and other system diseases 87.1%(54/62).Radical operation was performed in 51.6%(32/62) of the patients.Misdiagnosis and delayed diagnosis were common in old patient which would lead to poor prognosis.Conclusions: Early symptoms should be paid attention to Routine examinations such as blood test,stool test,rectal touch,colonoscopy and CT scan are recommended,as early detection and early treatment can improve the clinical outcome.
Objective: To clarify the features of colorectal carcinoma in patients over the age of 70 years.Methods: The clinical data of 62 patients(over 70 years of age) admitted from 2000 to 2002 were analyzed retrospectively.Results: Of the 62 cases,the incidence of Dukes C or D stage accounted for 62.9%(39/62),acute obstructing colorectal carcinoma 29.8%(18/62) and other system diseases 87.1%(54/62).Radical operation was performed in 51.6%(32/62) of the patients.Misdiagnosis and delayed diagnosis were common in old patient which would lead to poor prognosis.Conclusions: Early symptoms should be paid attention to Routine examinations such as blood test,stool test,rectal touch,colonoscopy and CT scan are recommended,as early detection and early treatment can improve the clinical outcome.
2004, 29(2): 128-130.
Abstract:
Objective: To study the effect of midazolam through different ways of administration.Methods: Forty ASA Ⅰ to Ⅱ patients scheduled for elective surgery were enrolled in this study.They were randomly divided into four groups with 10 cases in each group.They were intravenous group(V group),intramuscular group(M group),intranasal group(N group) and subligual group(S group).Each group was given midazolam 50 μg/kg for premedication by different ways of administration.The depth of sedation was monitored by BIS and modified by OAA/S sedation score,VAS score and amnesia.Results: The OAA/S score:70% of the patients in two groups(V,M) became unconscious.However,only 30% of the patients in the other groups(N,S) became unconscious.The BIS index decreased to 84.3±2.17 in V group,89.9±1.23 in M group,91.1±0.84 in N group and 91.3±0.61 in S group.The BIS index in group(V,M,N,S) decreased slightly 5 min,25 min,35 min and 35 min after administration.There were significant changes in the VAS score after giving premedication in V,M and N group(P<0.05 to P<0.01).The percentage of amnesia changed significant(P<0.01).Conclusions: The effects of sedation and amnesia of midazolam administered in a dose of 50 μg/kg are better in V and M groups than in N and S groups.
Objective: To study the effect of midazolam through different ways of administration.Methods: Forty ASA Ⅰ to Ⅱ patients scheduled for elective surgery were enrolled in this study.They were randomly divided into four groups with 10 cases in each group.They were intravenous group(V group),intramuscular group(M group),intranasal group(N group) and subligual group(S group).Each group was given midazolam 50 μg/kg for premedication by different ways of administration.The depth of sedation was monitored by BIS and modified by OAA/S sedation score,VAS score and amnesia.Results: The OAA/S score:70% of the patients in two groups(V,M) became unconscious.However,only 30% of the patients in the other groups(N,S) became unconscious.The BIS index decreased to 84.3±2.17 in V group,89.9±1.23 in M group,91.1±0.84 in N group and 91.3±0.61 in S group.The BIS index in group(V,M,N,S) decreased slightly 5 min,25 min,35 min and 35 min after administration.There were significant changes in the VAS score after giving premedication in V,M and N group(P<0.05 to P<0.01).The percentage of amnesia changed significant(P<0.01).Conclusions: The effects of sedation and amnesia of midazolam administered in a dose of 50 μg/kg are better in V and M groups than in N and S groups.
2004, 29(2): 130-131.
Abstract:
Objective: To investigate the clinical manifestations and histopathologic features of the tumoral calcinosis at active stage.Methods: The clinicopathologic features of 13 cases of tumoral calcinosis at active stage were retrospectively analysed.Results: Of the 13 cases diagnosed as tumoral calcinosis at active stage,7 were men and 6 were women.The age ranged from 16 to 65 years and the course of disease was 1 to 11 years.Multiple masses were found in 3 cases and solitary nodule in 10 cases.Conclusions: The clinical symptom of the tumoral calcinosis at active stage is painless masses.The masses develope in the parosteal or juxta-articular part,not invading the bone or the articulation.X-ray shows characteristic calcific shadow.The pathologic manifestations are multi cystic or solid nodules.The cyst wall is lined by the granulation tissues and the internal capsular contains the amorphous calcium salt granule.
Objective: To investigate the clinical manifestations and histopathologic features of the tumoral calcinosis at active stage.Methods: The clinicopathologic features of 13 cases of tumoral calcinosis at active stage were retrospectively analysed.Results: Of the 13 cases diagnosed as tumoral calcinosis at active stage,7 were men and 6 were women.The age ranged from 16 to 65 years and the course of disease was 1 to 11 years.Multiple masses were found in 3 cases and solitary nodule in 10 cases.Conclusions: The clinical symptom of the tumoral calcinosis at active stage is painless masses.The masses develope in the parosteal or juxta-articular part,not invading the bone or the articulation.X-ray shows characteristic calcific shadow.The pathologic manifestations are multi cystic or solid nodules.The cyst wall is lined by the granulation tissues and the internal capsular contains the amorphous calcium salt granule.
2004, 29(2): 132-134.
Abstract:
Objective: To explore the diagnostic value of serum tumor specific growth factor(TSGF),carcinoembryonic antigen(CEA),CA242 and CA19-9 combined in detecting malignant tumors of digestive tract.Methods: The levels of serum TSGF,CEA,CA242 and CA19-9 were determined by biochemical or immunoradiometric assay in patients with malignant tumors of digestive tract and in control group.Results: The sensitivity rates of using single positive diagnostic indicator of TSGF,CEA,CA242 and CA19-9 to malignant tumors of digestive tract were 79.24%,31.78%,42.80% and 55.51%,respectively.The specific rates were 82.76%,97.84%,98.71% and 92.24%,respectively.The accuracy rates were 80.98%,64.53%,70.51% and 73.72%,respectively.In combined detection,the diagnostic sensitivity rate was 90.25%,the specific rate was 72.41%,and the accuracy rate was 81.41%.Conclusions: Detecting serum TSGF,CEA,CA242 and CA19-9 combinedly is of great clinical value for the diagnosis of malignant tumors of digestive tract.
Objective: To explore the diagnostic value of serum tumor specific growth factor(TSGF),carcinoembryonic antigen(CEA),CA242 and CA19-9 combined in detecting malignant tumors of digestive tract.Methods: The levels of serum TSGF,CEA,CA242 and CA19-9 were determined by biochemical or immunoradiometric assay in patients with malignant tumors of digestive tract and in control group.Results: The sensitivity rates of using single positive diagnostic indicator of TSGF,CEA,CA242 and CA19-9 to malignant tumors of digestive tract were 79.24%,31.78%,42.80% and 55.51%,respectively.The specific rates were 82.76%,97.84%,98.71% and 92.24%,respectively.The accuracy rates were 80.98%,64.53%,70.51% and 73.72%,respectively.In combined detection,the diagnostic sensitivity rate was 90.25%,the specific rate was 72.41%,and the accuracy rate was 81.41%.Conclusions: Detecting serum TSGF,CEA,CA242 and CA19-9 combinedly is of great clinical value for the diagnosis of malignant tumors of digestive tract.
2004, 29(2): 134-135.
Abstract:
Objective: To study the changs of immune-molecular CD35,CD44s and CD58 expressions on erythrocyte in patients with chronic renal failure and their significance.Methods: CD35,CD44s and CD58 expressions on erythrocyte in 30 patients with chronic renal failure(CRF) and 50 healthy controls were measured by the erythrocyte enzyme immunization method for quantitatively assaying complete erythrocyte.Results: The quantity of CD35,CD44s and CD58 on erythrocyte in the CRF group was significantly lower than that in the healthy group(P<0.001).The quantity of CD35,CD44s and CD58 on erythrocyte of dialysed patients increased obviously compared with that of the patients without being dialysed,but the difference between the two groups was not significant(P>0.05).Conclusions: The molecular CD35,CD44s and CD58 expressed on erythrocyte decreas significantly in patients with CRF especially CD35(CR1).It suggests that the complements-mediated adhesion functions are possibly damaged.It may be one of the reasons that the CRF patients are easily infected.
Objective: To study the changs of immune-molecular CD35,CD44s and CD58 expressions on erythrocyte in patients with chronic renal failure and their significance.Methods: CD35,CD44s and CD58 expressions on erythrocyte in 30 patients with chronic renal failure(CRF) and 50 healthy controls were measured by the erythrocyte enzyme immunization method for quantitatively assaying complete erythrocyte.Results: The quantity of CD35,CD44s and CD58 on erythrocyte in the CRF group was significantly lower than that in the healthy group(P<0.001).The quantity of CD35,CD44s and CD58 on erythrocyte of dialysed patients increased obviously compared with that of the patients without being dialysed,but the difference between the two groups was not significant(P>0.05).Conclusions: The molecular CD35,CD44s and CD58 expressed on erythrocyte decreas significantly in patients with CRF especially CD35(CR1).It suggests that the complements-mediated adhesion functions are possibly damaged.It may be one of the reasons that the CRF patients are easily infected.
2004, 29(2): 136-138.
Abstract:
Objective: To investigate the cause of failure in all endodontically treated teeth that were evertually extracted.Methods: The clinical data of 121 extracted teeth which had previously received endodontic therapy were analysed.Results: Of the teeth, 61.2% were prosthetic failures which were due primarily to crown fracture.29.8% were periodontal failures. Only 9.0% of the failure were due to endodontic causes.Conclusions: Teeth that had been crowned have greater longevity than the uncrowned ones.The endodontic failure is rarelly seen,but once it occurs,the consequences are serious.
Objective: To investigate the cause of failure in all endodontically treated teeth that were evertually extracted.Methods: The clinical data of 121 extracted teeth which had previously received endodontic therapy were analysed.Results: Of the teeth, 61.2% were prosthetic failures which were due primarily to crown fracture.29.8% were periodontal failures. Only 9.0% of the failure were due to endodontic causes.Conclusions: Teeth that had been crowned have greater longevity than the uncrowned ones.The endodontic failure is rarelly seen,but once it occurs,the consequences are serious.
2004, 29(2): 138-139.
Abstract:
Objective: To present the cosmetic use of botulinum A toxin in treatment of dynamic facial lines.Methods: Botulinum A toxin was injected for treatment of dynamic facial lines.Results: The effect was satisfactory,leaving little trauma.Conclusions: It is safe and effective to treat dynamic lines of the face with botulinum A toxin.
Objective: To present the cosmetic use of botulinum A toxin in treatment of dynamic facial lines.Methods: Botulinum A toxin was injected for treatment of dynamic facial lines.Results: The effect was satisfactory,leaving little trauma.Conclusions: It is safe and effective to treat dynamic lines of the face with botulinum A toxin.
2004, 29(2): 140-142.
Abstract:
Objective: To explore the feasibility,safety and clinical effects of early postoperative enteral nutrition(EN) for patients with esophageal cancer.Methods: Eighty cases of esophageal cancer were randomly divided into EN group(n=42) and parenteral nutrition(PN) group(n=38).A series of parameters including serum total proteins,serum albumin,prealbumin,transferrin,lymphocyte count,urea nitrogen,creatinine,serum glucose and glutamic pyruvic transaminase were tested preoperatively and at postoperative day 7.The sympotom of digestive tract,resumption of gastrointestinal functions,postoperative complication and length of hospital stay were carefully observed and recorded.Results: No mortality occurred during the period of study.There was no evidence of damage of EN to the renal or liver function.The concentration of prealbumin,transferrin and lymphocyte count was significantly elevated after nutritional support in EN group(P<0.01).Compared with PN group,the concentration of serum albumin,prealbumin,transferrin and lymphocyte count was significantly increased after nutritional support in EN group(P<0.01).The early out of bed activity and the bowel movement resumed significantly earlier in EN group than those in PN group(P<0.001).The length of hospital stay was shorter in EN group(P<0.01) and there was no serious complication in EN group.Conclusions: Early postoperative enteral nutrition for patients with esophageal cancer is safe,feasible and effective.The early enteral nutrition is beneficial to the improvement of nutritional status,resumption of immunological function,reduction of the postoperative complication and improvement of the quality of life.
Objective: To explore the feasibility,safety and clinical effects of early postoperative enteral nutrition(EN) for patients with esophageal cancer.Methods: Eighty cases of esophageal cancer were randomly divided into EN group(n=42) and parenteral nutrition(PN) group(n=38).A series of parameters including serum total proteins,serum albumin,prealbumin,transferrin,lymphocyte count,urea nitrogen,creatinine,serum glucose and glutamic pyruvic transaminase were tested preoperatively and at postoperative day 7.The sympotom of digestive tract,resumption of gastrointestinal functions,postoperative complication and length of hospital stay were carefully observed and recorded.Results: No mortality occurred during the period of study.There was no evidence of damage of EN to the renal or liver function.The concentration of prealbumin,transferrin and lymphocyte count was significantly elevated after nutritional support in EN group(P<0.01).Compared with PN group,the concentration of serum albumin,prealbumin,transferrin and lymphocyte count was significantly increased after nutritional support in EN group(P<0.01).The early out of bed activity and the bowel movement resumed significantly earlier in EN group than those in PN group(P<0.001).The length of hospital stay was shorter in EN group(P<0.01) and there was no serious complication in EN group.Conclusions: Early postoperative enteral nutrition for patients with esophageal cancer is safe,feasible and effective.The early enteral nutrition is beneficial to the improvement of nutritional status,resumption of immunological function,reduction of the postoperative complication and improvement of the quality of life.
2004, 29(2): 143-144.
Abstract:
Objective: To study the application of T-model or L-model steel plate in treatment of complicated fracture of tibial plateau.Methods: Eighteen cases were treated by surgery from 1997 to 2002.The fracture was fixed with the T-model or L-model steel plate,the intact articular surface recovered and the spongy bone or artificial bone below the collapse of articular surface implanted simultaneously.Results: The follow-up time was 6 to 32 months and the curative effects were good.Conclusions: The complicated fracture of tibial plateau can be treated by open reduction,fixation with T-model or L-model steel plate and bone grafting.
Objective: To study the application of T-model or L-model steel plate in treatment of complicated fracture of tibial plateau.Methods: Eighteen cases were treated by surgery from 1997 to 2002.The fracture was fixed with the T-model or L-model steel plate,the intact articular surface recovered and the spongy bone or artificial bone below the collapse of articular surface implanted simultaneously.Results: The follow-up time was 6 to 32 months and the curative effects were good.Conclusions: The complicated fracture of tibial plateau can be treated by open reduction,fixation with T-model or L-model steel plate and bone grafting.
2004, 29(2): 145-147.
Abstract:
Objective: To investigate the effect of gastric pacing on gastric electrical activity in patients with gastric dysrhythmias.Methods: Thirteen patients with gastric dysrhythmias were examined with electrogastrography (EGG),and their symptoms were assessed before and after gastric pacing treatment.Results: The patients'symptoms were significantly improved after gastric pacing treatment.The total response rate was 90.05%.The percentage of preprandial normal slow wave before and after treatment was (41.9±16.9)% and (46.9±21.6)%(P>0.05) respectively,with no statistical difference.The percentage of postprandial normal slow wave before and after treatment was (57.5±28.6)% and (78.7±16.6)%(P<0.05) respectively,with significantly statistical difference.Conclusions: Gastric pacing could improve gastric electrodysrhythemia and ameliorate symptoms in shorttime for patients with gastric motility disorders.
Objective: To investigate the effect of gastric pacing on gastric electrical activity in patients with gastric dysrhythmias.Methods: Thirteen patients with gastric dysrhythmias were examined with electrogastrography (EGG),and their symptoms were assessed before and after gastric pacing treatment.Results: The patients'symptoms were significantly improved after gastric pacing treatment.The total response rate was 90.05%.The percentage of preprandial normal slow wave before and after treatment was (41.9±16.9)% and (46.9±21.6)%(P>0.05) respectively,with no statistical difference.The percentage of postprandial normal slow wave before and after treatment was (57.5±28.6)% and (78.7±16.6)%(P<0.05) respectively,with significantly statistical difference.Conclusions: Gastric pacing could improve gastric electrodysrhythemia and ameliorate symptoms in shorttime for patients with gastric motility disorders.
2004, 29(2): 147-148.
Abstract:
Objective: To evaluate the therapeutic effect and side effect of high dose of cisplatin(HD DDP) combined with fluorouracil(5-Fu)/leucovorin(CF) plus medroxyprogesterone acetate(MPA) on advanced gastric and colorectal adenocarcinoma.Methods: Twenty three cases of advanced gastric adenocarcinoma cases and 14 cases of advanced colorectal adenocarcinoma were treated with HD-DDP+5-Fu/CF+MPA regimen.Results: Effectiveness was noted in 10 cases in gastric adenocarcinoma group and 5 cases in the other;the overall responses rate(CR+PR) was 40.5% and 35.7% respectively.The main side-effects were nausea and vomiting,which reached degree Ⅱ or higher(24.3%).BUN elevation was observed only in two cases.Conclusions: HD-DDP+5-Fu/CF+MPA regimen proves to be effective and its side-effects are slight and endurable.
Objective: To evaluate the therapeutic effect and side effect of high dose of cisplatin(HD DDP) combined with fluorouracil(5-Fu)/leucovorin(CF) plus medroxyprogesterone acetate(MPA) on advanced gastric and colorectal adenocarcinoma.Methods: Twenty three cases of advanced gastric adenocarcinoma cases and 14 cases of advanced colorectal adenocarcinoma were treated with HD-DDP+5-Fu/CF+MPA regimen.Results: Effectiveness was noted in 10 cases in gastric adenocarcinoma group and 5 cases in the other;the overall responses rate(CR+PR) was 40.5% and 35.7% respectively.The main side-effects were nausea and vomiting,which reached degree Ⅱ or higher(24.3%).BUN elevation was observed only in two cases.Conclusions: HD-DDP+5-Fu/CF+MPA regimen proves to be effective and its side-effects are slight and endurable.
2004, 29(2): 149-151.
Abstract:
Objective: To study the risk factors for secondary caries in posterior teeth,and to provide ways of prevention.Methods: One thousand five hundred teeth with caries restoration were collected and 426 teeth which developed secondary caries were analyzed.Results: Secondary caries were obviously related to the filling time,material,position,oral hygiene status,the preparation of caves and so on(P<0.005).Conclusions: Improper materia time,position,oral hygiene status and the preparation of caves are the risk factors for secondary caries.
Objective: To study the risk factors for secondary caries in posterior teeth,and to provide ways of prevention.Methods: One thousand five hundred teeth with caries restoration were collected and 426 teeth which developed secondary caries were analyzed.Results: Secondary caries were obviously related to the filling time,material,position,oral hygiene status,the preparation of caves and so on(P<0.005).Conclusions: Improper materia time,position,oral hygiene status and the preparation of caves are the risk factors for secondary caries.
2004, 29(2): 151-153.
Abstract:
Objective: To examine the relationship between hyperhomocysteinemia,cerebrovascular disease and the relative factors.Methods: The four groups randomly selected had no obvious differences in age and sex.The plasma homocysteine(Hcy) levels of 60 patients with cerebral infarction,30 patients with cerebral hemorrhage,30 patients with other neurological diseases and 30 normal controls were measured by STE.Besides Hcy,each patient received laboratory examinations including folate and Vitamin B12.Results: The plasma Hcy level in the group with cerebral infarction or cerebral hemorrhage was significantly higher than that with other neurological diseases or normal controls(P<0.01).Correlation analysis showed that plasma Hcy in stroke was negatively correlated with plasma folate and Vitamin B12 (P<0.05).Conclusions: There is a certain relationship between the level of Hcy and cerebrovascular disease;therefore folate and Vitamin B12 supplementation might help to lower the risk factors.
Objective: To examine the relationship between hyperhomocysteinemia,cerebrovascular disease and the relative factors.Methods: The four groups randomly selected had no obvious differences in age and sex.The plasma homocysteine(Hcy) levels of 60 patients with cerebral infarction,30 patients with cerebral hemorrhage,30 patients with other neurological diseases and 30 normal controls were measured by STE.Besides Hcy,each patient received laboratory examinations including folate and Vitamin B12.Results: The plasma Hcy level in the group with cerebral infarction or cerebral hemorrhage was significantly higher than that with other neurological diseases or normal controls(P<0.01).Correlation analysis showed that plasma Hcy in stroke was negatively correlated with plasma folate and Vitamin B12 (P<0.05).Conclusions: There is a certain relationship between the level of Hcy and cerebrovascular disease;therefore folate and Vitamin B12 supplementation might help to lower the risk factors.
2004, 29(2): 154-155.
Abstract:
Objective: To investigate the accuracy of electronic root canal length measuring methods by JUSTY-Ⅱ full auto root apex locator.Methods: JUSTY-Ⅱ full auto root apex locator was used to detect the apical foramen of 130 teeth and the results were compared with the X-ray dental pictures.Results: An accurate location ±0.5 mm of the apex was obtained in 87.7% of the cases.Conclusions: Determining root canal length by full auto apex locator could attain good accuracy,reduce X-ray harm to patients,increase success rate of root canal treatment and decrease clinical time.
Objective: To investigate the accuracy of electronic root canal length measuring methods by JUSTY-Ⅱ full auto root apex locator.Methods: JUSTY-Ⅱ full auto root apex locator was used to detect the apical foramen of 130 teeth and the results were compared with the X-ray dental pictures.Results: An accurate location ±0.5 mm of the apex was obtained in 87.7% of the cases.Conclusions: Determining root canal length by full auto apex locator could attain good accuracy,reduce X-ray harm to patients,increase success rate of root canal treatment and decrease clinical time.
2004, 29(2): 156-157.
Abstract:
Objective: To observe the application of combined spinal epidural anesthesia in shysterectomy.Methods: Fourty patiens with Ⅰ to Ⅱ grade of ASA received shysterectomy under combined spinal epidural anesthesia(CSEA),and the mean arterial pressure(MAP),heart rate(HR),pulse oxygen saturation(SpO2) were monitored continuously.The changes of MAP,HR,SpO2 at the points of pre-anesthesia,post-anesthesia,pre-operation and post-operation were recorded.Results: The anesthetic effect in all patients was satisfactory.The onset of anesthesia was (8.15±2.2)min.There was no difference in MAP or SpO2 at the four time points(P>0.05),while HR was significantly different between pre anesthesia and post-anesthesia(P<0.01),but at other time points,HR had no significant changes(P>0.05).There was no nausea or vomiting during the operation and no headache occurred after spinal anesthesia.Conclusions: CSEA has the advantages of less administration of local anesthetic,perfect nerve block,and little influence on the circulation system.It fits for shysterectomy.
Objective: To observe the application of combined spinal epidural anesthesia in shysterectomy.Methods: Fourty patiens with Ⅰ to Ⅱ grade of ASA received shysterectomy under combined spinal epidural anesthesia(CSEA),and the mean arterial pressure(MAP),heart rate(HR),pulse oxygen saturation(SpO2) were monitored continuously.The changes of MAP,HR,SpO2 at the points of pre-anesthesia,post-anesthesia,pre-operation and post-operation were recorded.Results: The anesthetic effect in all patients was satisfactory.The onset of anesthesia was (8.15±2.2)min.There was no difference in MAP or SpO2 at the four time points(P>0.05),while HR was significantly different between pre anesthesia and post-anesthesia(P<0.01),but at other time points,HR had no significant changes(P>0.05).There was no nausea or vomiting during the operation and no headache occurred after spinal anesthesia.Conclusions: CSEA has the advantages of less administration of local anesthetic,perfect nerve block,and little influence on the circulation system.It fits for shysterectomy.
2004, 29(2): 158-159.
Abstract:
Objective: To investigate the clinical and MRI features of demyelinating in children disease.Methods: Thirty eight cases of demyelinating disease were analyzed retrospectively.Results: The brain MRI of 20 cases with multiple selerosis and 18 cases with acute disseminated encephalomyelitis were all abnormal.MRI showed T1W1isosignal or low signal focus and T2W1 high signal focus in the matter,basal nuclci,brain stem and cerebellum.Conclusions: Demylinizing disease in children dispays diversified clinical manifestation.MRI may be used as a means for early diagnosis of demyelinating in children. Back
Objective: To investigate the clinical and MRI features of demyelinating in children disease.Methods: Thirty eight cases of demyelinating disease were analyzed retrospectively.Results: The brain MRI of 20 cases with multiple selerosis and 18 cases with acute disseminated encephalomyelitis were all abnormal.MRI showed T1W1isosignal or low signal focus and T2W1 high signal focus in the matter,basal nuclci,brain stem and cerebellum.Conclusions: Demylinizing disease in children dispays diversified clinical manifestation.MRI may be used as a means for early diagnosis of demyelinating in children. Back
2004, 29(2): 172-173.
Abstract:
Objective: To compare B-Flow and color Doppler flow imaging(CDFI) in the detection of internal carotid artery.Methods: The internal carotid artery of forty normal volunteers was detected by B-Flow and CDFI.Results: B-Flow image showed that the artery wall was distinct,the blood was well distributed and identical,while the CDFI image showed overflow color in the near section and color insufficient in the middle and distant section.The B-Flow group was better than the CDFI group in the demonstration of range of internal carotid artery(25.5±5.6)mm and (14.5±7.8)mm(P<0.001).Conclusions: B-Flow can display wider-range of internal carotid artery than CDFI,and can make up for the inadequacy of CDFI in the detection of internal carotid artery.
Objective: To compare B-Flow and color Doppler flow imaging(CDFI) in the detection of internal carotid artery.Methods: The internal carotid artery of forty normal volunteers was detected by B-Flow and CDFI.Results: B-Flow image showed that the artery wall was distinct,the blood was well distributed and identical,while the CDFI image showed overflow color in the near section and color insufficient in the middle and distant section.The B-Flow group was better than the CDFI group in the demonstration of range of internal carotid artery(25.5±5.6)mm and (14.5±7.8)mm(P<0.001).Conclusions: B-Flow can display wider-range of internal carotid artery than CDFI,and can make up for the inadequacy of CDFI in the detection of internal carotid artery.
2004, 29(2): 174-175.
Abstract:
Objective: To study whether the value of QT interval dispersion(QTd) increases along with precipitation of myocardial ischemia in transesophagus atrial pacing heart stress test.Methods: The QTd was measured in patients with coronary artery disease and normal subjects before stress test,during stress test,when the pacing heart rate achieved the maximum,the moment and two minutes after stress test.Results: QTd of the maximal pacing heart rate during stress test,the moment and two minutes after stress test increased significantly(P<0.01) compared with those before stress test in patients with cornary artery disease.QTd of the maximal pacing heart rate during stress test,the moment and two minutes after stress test did not increase significantly(P>0.05) compared with those before stress test in normal subjects.Conclusions: It suggests that increase of QTd might be used as an index to judge coronary artery disease in transesophagus atrial pacing heart stress test.
Objective: To study whether the value of QT interval dispersion(QTd) increases along with precipitation of myocardial ischemia in transesophagus atrial pacing heart stress test.Methods: The QTd was measured in patients with coronary artery disease and normal subjects before stress test,during stress test,when the pacing heart rate achieved the maximum,the moment and two minutes after stress test.Results: QTd of the maximal pacing heart rate during stress test,the moment and two minutes after stress test increased significantly(P<0.01) compared with those before stress test in patients with cornary artery disease.QTd of the maximal pacing heart rate during stress test,the moment and two minutes after stress test did not increase significantly(P>0.05) compared with those before stress test in normal subjects.Conclusions: It suggests that increase of QTd might be used as an index to judge coronary artery disease in transesophagus atrial pacing heart stress test.
2004, 29(2): 176-178.
Abstract:
Objective: To investigate the significance of anti-DNP antibody,anti Smith antibody and anti-double stain DNA antibody in the patient with systemic lupus erythematosus(SLE).Methods: Anti-Smith antibody was detected by immuno-blotting technique,anti-double stain DNA antibody by dot immunofiltration assay,and anti-DNP antibody by latex immunoassay.Results: Among the 57 cases,the positive rates of anti-DNP antibody,anti-Smith antibody, and anti-double stain DNA antibody were 73.7%,33.3% and 61.4%,respectively.The total positive rate was 94.7%.Conclusions: In diagnosing SLE,allied examination of anti-DNP antibody,anti Smith antibody,anti-double stain DNA antibody can upraise the positive rate.The above tests are important in tracing patient's condition and judging the prognosis of SLE.Detecting serum anti-DNP can directly improve the detection rate of SLE instead of LE cell(LEC) detection.
Objective: To investigate the significance of anti-DNP antibody,anti Smith antibody and anti-double stain DNA antibody in the patient with systemic lupus erythematosus(SLE).Methods: Anti-Smith antibody was detected by immuno-blotting technique,anti-double stain DNA antibody by dot immunofiltration assay,and anti-DNP antibody by latex immunoassay.Results: Among the 57 cases,the positive rates of anti-DNP antibody,anti-Smith antibody, and anti-double stain DNA antibody were 73.7%,33.3% and 61.4%,respectively.The total positive rate was 94.7%.Conclusions: In diagnosing SLE,allied examination of anti-DNP antibody,anti Smith antibody,anti-double stain DNA antibody can upraise the positive rate.The above tests are important in tracing patient's condition and judging the prognosis of SLE.Detecting serum anti-DNP can directly improve the detection rate of SLE instead of LE cell(LEC) detection.
2004, 29(2): 178-179.
Abstract:
Objective: To study the relation between acute myocardial infarction(AMI),cerebral infarction(CI) and serum uric acid(UA).Methods: The serum uric acid of 36 patients with AMI and 48 patients with CI was determined and the results were compared with that of 40 normal controls.Results: The level of UA of patients with AMI(male 428.76±22.10 μmol/L;female 380.21±18.35 μmol/L) was markedly higher than that of normal controls(P<0.01).The level of UA of patients with CI(male 396.91±17.30 μmol/L;female 323.46±16.34 μmol/L) was also markedly higher than that of normal controls(P<0.01).The difference between group AMI and CI was significant(P<0.01).Conclusions: The increase of UA may be one of the risk factors for AMI and CI.
Objective: To study the relation between acute myocardial infarction(AMI),cerebral infarction(CI) and serum uric acid(UA).Methods: The serum uric acid of 36 patients with AMI and 48 patients with CI was determined and the results were compared with that of 40 normal controls.Results: The level of UA of patients with AMI(male 428.76±22.10 μmol/L;female 380.21±18.35 μmol/L) was markedly higher than that of normal controls(P<0.01).The level of UA of patients with CI(male 396.91±17.30 μmol/L;female 323.46±16.34 μmol/L) was also markedly higher than that of normal controls(P<0.01).The difference between group AMI and CI was significant(P<0.01).Conclusions: The increase of UA may be one of the risk factors for AMI and CI.
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