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2005 Vol. 30, No. 3
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2005, 30(3): 189-192.
Abstract:
Objective: To establish a constant model of malignant tumor cell differentiation and to set up a set of methods that the differentiated cells can be identified.Methods: HL-60 cells influenced by all-trans retinoic acid(ATRA) capable of inducing differentiation at different time were used as subjects.The differentiation marker on the cell surface and the cell volume were analyzed by using flow cytometry.The differentiated cells were identified by confocal microscope after having been stained with PI.Results: With the drug-inducing time increasing,the volume of differentiated cells was enlarged gradually.After 72 hours,the differentiated cells induced by ATRA began to express differentiating marker CD11b and the differentiated cells,nuclei was changed into the nucleus morph differentiation just like nutrophil nuclei.Conclusions: The differentiation of HL-60 cells can be induced by ATRA.HL-60 cells,differentiation can be conveniently judged by combining the cell svolume,and the cells differentiating marker,analyzed by flow cytometry with morphodifferentiation observed by confocal microscope after having been stained with propidium iodide.
Objective: To establish a constant model of malignant tumor cell differentiation and to set up a set of methods that the differentiated cells can be identified.Methods: HL-60 cells influenced by all-trans retinoic acid(ATRA) capable of inducing differentiation at different time were used as subjects.The differentiation marker on the cell surface and the cell volume were analyzed by using flow cytometry.The differentiated cells were identified by confocal microscope after having been stained with PI.Results: With the drug-inducing time increasing,the volume of differentiated cells was enlarged gradually.After 72 hours,the differentiated cells induced by ATRA began to express differentiating marker CD11b and the differentiated cells,nuclei was changed into the nucleus morph differentiation just like nutrophil nuclei.Conclusions: The differentiation of HL-60 cells can be induced by ATRA.HL-60 cells,differentiation can be conveniently judged by combining the cell svolume,and the cells differentiating marker,analyzed by flow cytometry with morphodifferentiation observed by confocal microscope after having been stained with propidium iodide.
2005, 30(3): 192-194.
Abstract:
Objective: To study dynamic expression of L-selctin(CD62L) in human peripheral γδT cells activated with Mycobacterium tuberculosis antigen(MtbAg) and cultured for proliferation.Methods: Peripheral blood mononuclear cells(PBMC) were stimulated with MtbAg to generate MtgAg activated T cells(MtbAT),or with CD3mAb activated αβT cells(CD3AT).Fresh isolation PBMC and cultured MtbAT or CD3AT for 3 to 21 days were stained with multicolor fluorescence mAbs.The percentage of CD62L+ cells among fresh PBMC,MtbAT or CD3AT were measured by flow cytometry.Results: The percentage of CD62L+ cells of γδT cells(43.9%) in fresh PBMC has significant lower than that of αβT cells(76.6%),the expression of CD62L arrived at climax on day 6 of culture in γδT cells(90.7%) and αβT cells(87.2%),after then the expression of CD72L decreased and maintained low level,the percentages of CD62L+ cells of γδU cells and αβT cells were 16.2% to 10.6% aod 23.6% to 17.5%,respectively,from day 15 to day 21 of culture.Conclusions: The CD62L expression of γδT cells in fresh PBMC was lower than that of αβTcells,but after activated by stimulation of MtbAg or CD3Ab,the expression of CD62L in human peripheral γδT cells,like αβTcells,rose in first and then decreased,and maintained low level later.
Objective: To study dynamic expression of L-selctin(CD62L) in human peripheral γδT cells activated with Mycobacterium tuberculosis antigen(MtbAg) and cultured for proliferation.Methods: Peripheral blood mononuclear cells(PBMC) were stimulated with MtbAg to generate MtgAg activated T cells(MtbAT),or with CD3mAb activated αβT cells(CD3AT).Fresh isolation PBMC and cultured MtbAT or CD3AT for 3 to 21 days were stained with multicolor fluorescence mAbs.The percentage of CD62L+ cells among fresh PBMC,MtbAT or CD3AT were measured by flow cytometry.Results: The percentage of CD62L+ cells of γδT cells(43.9%) in fresh PBMC has significant lower than that of αβT cells(76.6%),the expression of CD62L arrived at climax on day 6 of culture in γδT cells(90.7%) and αβT cells(87.2%),after then the expression of CD72L decreased and maintained low level,the percentages of CD62L+ cells of γδU cells and αβT cells were 16.2% to 10.6% aod 23.6% to 17.5%,respectively,from day 15 to day 21 of culture.Conclusions: The CD62L expression of γδT cells in fresh PBMC was lower than that of αβTcells,but after activated by stimulation of MtbAg or CD3Ab,the expression of CD62L in human peripheral γδT cells,like αβTcells,rose in first and then decreased,and maintained low level later.
2005, 30(3): 195-197.
Abstract:
Objective: To study the relationship between p53 gene mutation and the protein expression,and their roles in the early diagnosis and clinical significances in colorectal cancer.Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP),the mutations in exon 58 of p53 gene were examined in the tumor tissues and stool of 34 patients and 10 healthy controls.At the same time,using immunohistochemistry(IHC)(S-Pmethods),p53 protein expression were examined in the tumor tissues and normal mucosa.Results: The mutation of exon 58 and protein expression of p53 gene in the tissues were found in 18 of 34(52.9%) cases and 19 of 34(55.9%) cases,respectively,no mutation and protein expression were positive in normal mucosa(P<0.01).Of 12 patients who were positive for p53 gene mutation in tumor tissue,5(41.7%) had evidence of alterations in the p53 gene within the stool.The positive rates of p53 protein expression was correlates with the mutation of exon 58.The expression of p53 protein was correlated with tumor size(P<0.05),but not correlates to other cliniopathological factors.Conclusions: Our data indicate that gene mutation of p53 is one of the main factors which participate and affect the protein expression.p53 gene mutation can be detected within the stools.Detection of p53 gene in stool may provide new ways for the early diagnosis and census of high risk population in colorectal cancers.
Objective: To study the relationship between p53 gene mutation and the protein expression,and their roles in the early diagnosis and clinical significances in colorectal cancer.Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP),the mutations in exon 58 of p53 gene were examined in the tumor tissues and stool of 34 patients and 10 healthy controls.At the same time,using immunohistochemistry(IHC)(S-Pmethods),p53 protein expression were examined in the tumor tissues and normal mucosa.Results: The mutation of exon 58 and protein expression of p53 gene in the tissues were found in 18 of 34(52.9%) cases and 19 of 34(55.9%) cases,respectively,no mutation and protein expression were positive in normal mucosa(P<0.01).Of 12 patients who were positive for p53 gene mutation in tumor tissue,5(41.7%) had evidence of alterations in the p53 gene within the stool.The positive rates of p53 protein expression was correlates with the mutation of exon 58.The expression of p53 protein was correlated with tumor size(P<0.05),but not correlates to other cliniopathological factors.Conclusions: Our data indicate that gene mutation of p53 is one of the main factors which participate and affect the protein expression.p53 gene mutation can be detected within the stools.Detection of p53 gene in stool may provide new ways for the early diagnosis and census of high risk population in colorectal cancers.
2005, 30(3): 198-201.
Abstract:
Objective: To study the expression of MMP-2、MMP-9、TIMP-2 and infection of bacterial L-forms in ovarian neoplasms and to discuss the clinical significance.Methods: Immunohistochemical staining was used to detect the expression of MMP-2、MMP-9 and TIMP-2 in 120 case of ovarian tumors.Gram's straining was used to examine bacterial L-forms in these tissues.Results: The postive rates of MMP-2、MMP-9 and TIMP-2 were higher in malignant tumors than that in benign ones(P<0.005).The positive rates of MMP-2 and MMP-9 were higher in stage ⅠⅡ than that in stage Ⅲ,Ⅳ,and which increased with the grade (P<0.005).The expression of these two proteinases were higher in patients with lymph node involvement and ascites than those without that(P<0.05 to P<0.005).The positive expression of TIMP-2 was opposite to MMP-2 and MMP-9 (P<0.05 to P<0.005).The positive rate of bacterial L-forms was not higher in malignant tumors than that in benign ones(P>0.05),but it was increased with clinical stage and pathological grade (P<0.005).The expression of L-forms was higher in patients with lymph node involvement and ascites than those without that(P<0.005).Conclusions: In ovarian neoplasms,the abnormal expression of MMP-2、MMP-9、TIMP-2 and positive rate of bacterial L-forms have obvious relationship with clinical stage,pathological grade,lymph node involvement and ascites.Bacterial L-forms is maybe one of the inductive factors of the occurrence of tumor.MMP-2、MMP-9 and bacterial L-forms perhaps have the function of positive cooperativity during the course of the occurrence of ovarian neoplasms.TIMP-2 perhaps accelerate the development of ovarian neoplasms.
Objective: To study the expression of MMP-2、MMP-9、TIMP-2 and infection of bacterial L-forms in ovarian neoplasms and to discuss the clinical significance.Methods: Immunohistochemical staining was used to detect the expression of MMP-2、MMP-9 and TIMP-2 in 120 case of ovarian tumors.Gram's straining was used to examine bacterial L-forms in these tissues.Results: The postive rates of MMP-2、MMP-9 and TIMP-2 were higher in malignant tumors than that in benign ones(P<0.005).The positive rates of MMP-2 and MMP-9 were higher in stage ⅠⅡ than that in stage Ⅲ,Ⅳ,and which increased with the grade (P<0.005).The expression of these two proteinases were higher in patients with lymph node involvement and ascites than those without that(P<0.05 to P<0.005).The positive expression of TIMP-2 was opposite to MMP-2 and MMP-9 (P<0.05 to P<0.005).The positive rate of bacterial L-forms was not higher in malignant tumors than that in benign ones(P>0.05),but it was increased with clinical stage and pathological grade (P<0.005).The expression of L-forms was higher in patients with lymph node involvement and ascites than those without that(P<0.005).Conclusions: In ovarian neoplasms,the abnormal expression of MMP-2、MMP-9、TIMP-2 and positive rate of bacterial L-forms have obvious relationship with clinical stage,pathological grade,lymph node involvement and ascites.Bacterial L-forms is maybe one of the inductive factors of the occurrence of tumor.MMP-2、MMP-9 and bacterial L-forms perhaps have the function of positive cooperativity during the course of the occurrence of ovarian neoplasms.TIMP-2 perhaps accelerate the development of ovarian neoplasms.
2005, 30(3): 201-203.
Abstract:
Objective: To study the therapeutic effect of Rujianan capsule(RJAC) on mammary hyperplasia in rats.Methods: RJAC,a traditional Chinese medicine,was given to hyperplasia model of rats by per oral.The changes of mammary histocytes,function and sex hormone were observed.Results: RJAC can mend hyperplastic pathologic changes.It can reduce the volume and diameter of mammary gland,the number of breast leaflets and acini.The serum estrogen(E2) level and the serum progesterone(P) level were decreased(P<0.01).Conclusions: RJAC is effective in treatment of hyperplasia of mammary gland.
Objective: To study the therapeutic effect of Rujianan capsule(RJAC) on mammary hyperplasia in rats.Methods: RJAC,a traditional Chinese medicine,was given to hyperplasia model of rats by per oral.The changes of mammary histocytes,function and sex hormone were observed.Results: RJAC can mend hyperplastic pathologic changes.It can reduce the volume and diameter of mammary gland,the number of breast leaflets and acini.The serum estrogen(E2) level and the serum progesterone(P) level were decreased(P<0.01).Conclusions: RJAC is effective in treatment of hyperplasia of mammary gland.
2005, 30(3): 203-205.
Abstract:
Objective: To investigate the correlation of the expression of 67 kDa laminin receptor(67LR) with its biological behavior of gallbladder carcinoma.Methods: The 67LR protein expression in 40 cases of gallbladder carcinoma,21 cases of chronic cholecystitis and 9 cases of gallbladder adenoma were detected by immunohistochemistry SP method.Results: The positive expression rate of 67LR was 52.50% in the cases with gallbladder carcinoma,which was significantly higher than that with chronic cholecystitis(P<0.01),whereas no significant difference was detected between the cases with gallbladder adenoma or carcinoma(P>0.05).The positive expression rate of 67LR in gallbladder carcinoma with lymph node metastasis was significantly higher than that without metastasis((P<0.005)).Conclusions: 67LR may play an important role in both premalignant transformation of gallbladder adenoma and acquisition of the invasive and metastasis ability.
Objective: To investigate the correlation of the expression of 67 kDa laminin receptor(67LR) with its biological behavior of gallbladder carcinoma.Methods: The 67LR protein expression in 40 cases of gallbladder carcinoma,21 cases of chronic cholecystitis and 9 cases of gallbladder adenoma were detected by immunohistochemistry SP method.Results: The positive expression rate of 67LR was 52.50% in the cases with gallbladder carcinoma,which was significantly higher than that with chronic cholecystitis(P<0.01),whereas no significant difference was detected between the cases with gallbladder adenoma or carcinoma(P>0.05).The positive expression rate of 67LR in gallbladder carcinoma with lymph node metastasis was significantly higher than that without metastasis((P<0.005)).Conclusions: 67LR may play an important role in both premalignant transformation of gallbladder adenoma and acquisition of the invasive and metastasis ability.
2005, 30(3): 206-208.
Abstract:
Objective: To investigate the effects of minocycline(MC) on the growth and metastasis of lung carcinoma and tumor angiogenesis.Methods: T739 mice with lung carcinoma were used in this study.The rats were injected MC,cyclophosphamide(CTX) or normal saline(NS).The tumor growth delay and numbers of the metastatic foci on lungs and the expression of vascular endothelial growth factor(VEGF) of lung carcinoma were observed.Results: MC remarkably reduced the numbers of the metastatic foci and the expression of VEGF of lung carcinoma,and increased the tumor growth delay(P<0.01).Conclusions: MC can obviously inhibit the growth and metastasis of lung carcinoma in mice,and its mechanism is related to the expression of VEGF and angiogenesis.
Objective: To investigate the effects of minocycline(MC) on the growth and metastasis of lung carcinoma and tumor angiogenesis.Methods: T739 mice with lung carcinoma were used in this study.The rats were injected MC,cyclophosphamide(CTX) or normal saline(NS).The tumor growth delay and numbers of the metastatic foci on lungs and the expression of vascular endothelial growth factor(VEGF) of lung carcinoma were observed.Results: MC remarkably reduced the numbers of the metastatic foci and the expression of VEGF of lung carcinoma,and increased the tumor growth delay(P<0.01).Conclusions: MC can obviously inhibit the growth and metastasis of lung carcinoma in mice,and its mechanism is related to the expression of VEGF and angiogenesis.
2005, 30(3): 208-210.
Abstract:
Objective: To investigate the effect of etoposide(VP-16),in combination with oligodeoxynucleotides on lung adenocarcinoma cells.Methods: Etoposide in combination with telomerase 11-base oligodeoxynucleotides werer transfected into SPC-A1 cell line by lipofection.The multiparameters such as cell growth inhibition rate,cell cycle and apoptasis rate,and telomerase activity were detected by MTT,FCM,and PCR-ELISA,respectively.Results: Etoposide inhibited cell growth(with an inhibition rate of 18%),decreased telomerase activity and induced cell apoptosis.ASODN+VP-16 and SODN+VP-16 inhibit the growth of SPC-A1 cell line and rate are 43.5% and 41.0%,repectively(P>0.05).The difference was significant when compared with VP-16(P<0.01).Conclusions: Chemotherapeutant in combination with short chain oligodeoxynucleotides is more effective than used alone in inhibiting lung cancer and the side effect can be greatly reduced.
Objective: To investigate the effect of etoposide(VP-16),in combination with oligodeoxynucleotides on lung adenocarcinoma cells.Methods: Etoposide in combination with telomerase 11-base oligodeoxynucleotides werer transfected into SPC-A1 cell line by lipofection.The multiparameters such as cell growth inhibition rate,cell cycle and apoptasis rate,and telomerase activity were detected by MTT,FCM,and PCR-ELISA,respectively.Results: Etoposide inhibited cell growth(with an inhibition rate of 18%),decreased telomerase activity and induced cell apoptosis.ASODN+VP-16 and SODN+VP-16 inhibit the growth of SPC-A1 cell line and rate are 43.5% and 41.0%,repectively(P>0.05).The difference was significant when compared with VP-16(P<0.01).Conclusions: Chemotherapeutant in combination with short chain oligodeoxynucleotides is more effective than used alone in inhibiting lung cancer and the side effect can be greatly reduced.
2005, 30(3): 211-214.
Abstract:
Objective: To assess the value of detection of survivin mRNA in lung cancer tissues.Methods: The specimens and the para-carcinomatous normal tissues of 41 patients with lung cancer and 9 patients with benign pulmonary diseases were collected.Reverse transcriptase polymerase chain reaction(RT-PCR) was performed to detect the survivin mRNA expression in the specimens.All the patients were followed-up for 2 years.The results were analyzed relatively.Results: The positive rate of survivin mRNA in the carcinomatous tissues(70.7%) was higher than that in the para-carcinomatous normal tissues(17.1%) and the benign pulmonary diseases tissues(1/9)(P<0.005).There was no significant difference between the para-carcinomatous normal tissues and the benign pulmonary diseases tissues(P>0.05).survivin mRNA expression was not related to the age,sex,histological subtype,differentiation,pathologic grading or lymph node metastases.Conclusions: Expression of survivin mRNA in the lung cancer tissue can be used as a specific molecular marker for the diagnosis of lung cancer.It can act as an independent prognostic factor.
Objective: To assess the value of detection of survivin mRNA in lung cancer tissues.Methods: The specimens and the para-carcinomatous normal tissues of 41 patients with lung cancer and 9 patients with benign pulmonary diseases were collected.Reverse transcriptase polymerase chain reaction(RT-PCR) was performed to detect the survivin mRNA expression in the specimens.All the patients were followed-up for 2 years.The results were analyzed relatively.Results: The positive rate of survivin mRNA in the carcinomatous tissues(70.7%) was higher than that in the para-carcinomatous normal tissues(17.1%) and the benign pulmonary diseases tissues(1/9)(P<0.005).There was no significant difference between the para-carcinomatous normal tissues and the benign pulmonary diseases tissues(P>0.05).survivin mRNA expression was not related to the age,sex,histological subtype,differentiation,pathologic grading or lymph node metastases.Conclusions: Expression of survivin mRNA in the lung cancer tissue can be used as a specific molecular marker for the diagnosis of lung cancer.It can act as an independent prognostic factor.
2005, 30(3): 215-218.
Abstract:
Objective: To investigate the expressions of telomerase activity and mutatioms of p53 gene in sputa from patients with lung carcinomas and to determine whether the detections are of value in the early diagnosis of lung carcinomas.Methods: The expression of telomerase and mutations of p53 gene in sputa RT-PCR-PAGE and PCR-SSCP were employed respectively and the exfolieted cells were examined by standard papanicolaou staining.Results: The expression rate of telomerase activity in sputa from patients with lung was 58.3%and mutations of p53 gene were 43.3%.No telomerase activity and p53 gene mutations were detected in the sputa from patients with benign bronchial-pulmonary diseases.The difference was significant(P<0.05).There were significant differences between the exfolieted cells in sputa from patients with lung carcinomas,the expression of telomerase activity and the mutations of p53 gene(P<0.005-P<0.05).There were no statistical differences (P>0.05) between the mutations of p53 and the expression of telomerase activity with respect to pathological classifications and location of lung carcinomas.There was a positive correlation between the mutations of p53 gene and the expression of telomerase activity(P<0.01).Conclusions: The telomerase activity and the mutations p53 gene in sputa may serve as tumor markers for the early diagnosis of lung carcinomas.The expression rate of telomerase activity and the mutations rate of p53 gene are no statistically irrelevant with the location and pathological classifications of lung carcinomas.p53 gene may regulate the expression of telomerase activity.
Objective: To investigate the expressions of telomerase activity and mutatioms of p53 gene in sputa from patients with lung carcinomas and to determine whether the detections are of value in the early diagnosis of lung carcinomas.Methods: The expression of telomerase and mutations of p53 gene in sputa RT-PCR-PAGE and PCR-SSCP were employed respectively and the exfolieted cells were examined by standard papanicolaou staining.Results: The expression rate of telomerase activity in sputa from patients with lung was 58.3%and mutations of p53 gene were 43.3%.No telomerase activity and p53 gene mutations were detected in the sputa from patients with benign bronchial-pulmonary diseases.The difference was significant(P<0.05).There were significant differences between the exfolieted cells in sputa from patients with lung carcinomas,the expression of telomerase activity and the mutations of p53 gene(P<0.005-P<0.05).There were no statistical differences (P>0.05) between the mutations of p53 and the expression of telomerase activity with respect to pathological classifications and location of lung carcinomas.There was a positive correlation between the mutations of p53 gene and the expression of telomerase activity(P<0.01).Conclusions: The telomerase activity and the mutations p53 gene in sputa may serve as tumor markers for the early diagnosis of lung carcinomas.The expression rate of telomerase activity and the mutations rate of p53 gene are no statistically irrelevant with the location and pathological classifications of lung carcinomas.p53 gene may regulate the expression of telomerase activity.
2005, 30(3): 218-220.
Abstract:
Objective: To study the influence of N-acetylcysteine(NAC) on the growth and spontaneous metastasis of lung adenocarcinoma in T739 mice.Methods: Fifty-one T739 mice were randomly divided into 3 groups.Group Ⅰ acted as the control.Group Ⅱ and Ⅲ were administered different doses of NAC(0.5 g·kg-1·d-1,1.0 g·kg-1·d-1) in drinking water 72 hours before subcutaneous inoculation of LA795 lung adenocarcinoma cells(0.2×106),and then the formation and growth of the primary tumor,the number of lung metastasis and the concentration of glutathione(GSH)were observed.Results: On the fourth day after inoculation,the incidence of tumor was 81.25% in the control group,which was much higher than that of the treatment groups(47.1%,38.9%,respectively)(P<0.05).The weight of the tumor decreased significantly(P<0.05 and P<0.01) compared with the control group.The doses of NAC was negatively related to the weight of the local tumor(P<0.01).The influence of NAC on the number of lung metastasis was not statistically significant(P>0.05).The weight of the mice decreased greatly(P<0.01) compared to the initial weight in all the groups and the difference between the 3 groups was not significant(P>0.05).Conclusions: NAC can inhibit the formation of the local tumor and has no significant side-effects on the mice.It is a promising agent.
Objective: To study the influence of N-acetylcysteine(NAC) on the growth and spontaneous metastasis of lung adenocarcinoma in T739 mice.Methods: Fifty-one T739 mice were randomly divided into 3 groups.Group Ⅰ acted as the control.Group Ⅱ and Ⅲ were administered different doses of NAC(0.5 g·kg-1·d-1,1.0 g·kg-1·d-1) in drinking water 72 hours before subcutaneous inoculation of LA795 lung adenocarcinoma cells(0.2×106),and then the formation and growth of the primary tumor,the number of lung metastasis and the concentration of glutathione(GSH)were observed.Results: On the fourth day after inoculation,the incidence of tumor was 81.25% in the control group,which was much higher than that of the treatment groups(47.1%,38.9%,respectively)(P<0.05).The weight of the tumor decreased significantly(P<0.05 and P<0.01) compared with the control group.The doses of NAC was negatively related to the weight of the local tumor(P<0.01).The influence of NAC on the number of lung metastasis was not statistically significant(P>0.05).The weight of the mice decreased greatly(P<0.01) compared to the initial weight in all the groups and the difference between the 3 groups was not significant(P>0.05).Conclusions: NAC can inhibit the formation of the local tumor and has no significant side-effects on the mice.It is a promising agent.
2005, 30(3): 221-223.
Abstract:
Objective: To determine the inhibitory effects of cisplantin and sulfur-modified antisense oligodexyribonucleotide(ASODN) on the growth of transplantable lung carcinoma in mice and on the telomerase activity of carcinoma,liver and testis.Methods: Lewis lung glandular carcinoma cells(1×10P>7 P>/ml) were inoculated subcutaneously to 44 C57 male mice.They were randomized into 6 groups.From the 10th day on,0.5 ml 0.9%NS and antisense mTR were intraperitoneally given to group Ⅰ and groupⅡ,respectively,every day for 6 days;0.5 ml DDP of different concentration,1.0 mg/kg,2.0 mg/kg and 4.0 mg/kg,were intraperitoneally injected on the 15th day once in Ⅲ, Ⅳ and Ⅴ groups,respectively;DDP and ASODN combined were applied to group Ⅵ.The telomerase activities of tumor,liver and testis were determined sermi-quantatativly by TRAP-ELASA.Results: Volumes of mice transplantable carcinoma in groups Ⅴ and Ⅵ were greatly reduced(P<0.01) comparing with other groups.The telomerase activity of mice carcinoma in groups Ⅴ and Ⅵ was the lowest(P<0.01) in contrast to other groups.Mice liver and testis telomerase activity in groups Ⅳ,Ⅴand Ⅵ presented significant reduction(P<0.05-P<0.01) in contrast to health control group and groupⅠ.In addition,the liver and testis telomerase activity of group Ⅱ showed no significant reduction(P>0.05) in contrast to groupⅠand healthy contrast group.Conclusions: ASODN alone have no effects on tumor growth and liver and testis telomerase activity,but has inhibitory effect on the telomerase activity of transplantable lung carcinoma.Treatment with DDP alone or in combination with ASODN and DDP demonstrates apparent carcinoma volume shrinkage,carcinoma necrosis and telomerase activity inhibition.Combination of DDP and ASODN has cooperative effect on transplantable lung carcinoma.
Objective: To determine the inhibitory effects of cisplantin and sulfur-modified antisense oligodexyribonucleotide(ASODN) on the growth of transplantable lung carcinoma in mice and on the telomerase activity of carcinoma,liver and testis.Methods: Lewis lung glandular carcinoma cells(1×10
2005, 30(3): 224-225.
Abstract:
Objective: To study the influence of acanthopanax senticosus injection (ASI) on cerebral ischemic reperfusion injury in mice.Methods: The cerebral ischemic reperfusion injury model was established by binding the bilateral total artery and the soft tissue of neck in mice.The mice were randomly divided into 3 groups:control group,ischemic reperfusion group (I/R) and ASI group(AS).The electroencephalogram(EEG) was continually observed during the experiment course and the water content in cerebrum and the Evans blue content in brain organization were measured after the mice were killed.Results: The mice EEG in control group did not have any unusual change before and after operation;the mice EEG in I/R group and AS group was a straight line during the observing course;the water content in I/R group(83.92±5.10)% was higher than that in AS group(79.21±2.59)% (P<0.05);the Evans blue content in I/R group(0.535 7 ±0.098 8) was obvious higher than that in AS group(0.300 0 ±0.064 5) (P<0.01).Conclusions: The ASI plays a protective role in ischemic reperfusion injury in mice.
Objective: To study the influence of acanthopanax senticosus injection (ASI) on cerebral ischemic reperfusion injury in mice.Methods: The cerebral ischemic reperfusion injury model was established by binding the bilateral total artery and the soft tissue of neck in mice.The mice were randomly divided into 3 groups:control group,ischemic reperfusion group (I/R) and ASI group(AS).The electroencephalogram(EEG) was continually observed during the experiment course and the water content in cerebrum and the Evans blue content in brain organization were measured after the mice were killed.Results: The mice EEG in control group did not have any unusual change before and after operation;the mice EEG in I/R group and AS group was a straight line during the observing course;the water content in I/R group(83.92±5.10)% was higher than that in AS group(79.21±2.59)% (P<0.05);the Evans blue content in I/R group(0.535 7 ±0.098 8) was obvious higher than that in AS group(0.300 0 ±0.064 5) (P<0.01).Conclusions: The ASI plays a protective role in ischemic reperfusion injury in mice.
2005, 30(3): 226-227.
Abstract:
Objective: To observe the effect of taurine on the excitation of the sciatic nerve trunk in vitro.Methods: The sciatic nerve trunks of toads were isolated and soaked in Ringer's solution containing different concentration of chloride calcium with taurine(40 mmol/L) for 30 minutes.The threshold,refractory period and conduction velocities of action potential were measured by computer.Results: Compared with the normal Ringer's solution group (1.09 mmol/L),the threshold increased and the refractory period shrinked in the Ringer's solution containing higher concentration of chloride calcium(2.18 mmol/L),while the threshold and the conduction velocity of action potential decreased,the refractory period shrinked in the lower concentration(0.27 mmol/L)(P<0.05-P<0.01).Above changes are unobvious in Ringer's solution containing taurine(40 mmol/L) with lower or higher concentration of chloride calcium(P>0.05).Conclusions: The taurine could regulate the excitability of the sciatic nerve trunk in vitro.
Objective: To observe the effect of taurine on the excitation of the sciatic nerve trunk in vitro.Methods: The sciatic nerve trunks of toads were isolated and soaked in Ringer's solution containing different concentration of chloride calcium with taurine(40 mmol/L) for 30 minutes.The threshold,refractory period and conduction velocities of action potential were measured by computer.Results: Compared with the normal Ringer's solution group (1.09 mmol/L),the threshold increased and the refractory period shrinked in the Ringer's solution containing higher concentration of chloride calcium(2.18 mmol/L),while the threshold and the conduction velocity of action potential decreased,the refractory period shrinked in the lower concentration(0.27 mmol/L)(P<0.05-P<0.01).Above changes are unobvious in Ringer's solution containing taurine(40 mmol/L) with lower or higher concentration of chloride calcium(P>0.05).Conclusions: The taurine could regulate the excitability of the sciatic nerve trunk in vitro.
2005, 30(3): 228-229.
Abstract:
Objective: To investigate the effective means for the diagnosis and treatment of acute arterial embolism of the lower extremity complicated with myonephropathic metabolic syndrome(MMS).Methods: The extent of the welling and ischemia of the lower extremity,the change of creatine phosphokinase(CPK),serum glutamic-oxaloacetic transaminase(SGOT),lactat dehydrogeneise(LDH) and blood potassium were monitored.Creatinine BUN and pH of the blood were checked repeatedly.Once diagnosed,fasciotomy should be performed.In addition,alkalinization of the urine,oxygen-derived free radical scavengers should be administered.Results: Of the 48 patients with acute arterial embolism of the lower extremity,18 were complicated with MMS.One died,and the mortality rate was 11.1%;one case was amputated up the knee,and the rate of amputation was 5.6%.Conclusions: The rate of death or amputation might be reduced by embolectomy combined with the administration of vigorous hydration,alkalinization of the urine,oxygen-derived free radical scavengers and effective fasciotomy.
Objective: To investigate the effective means for the diagnosis and treatment of acute arterial embolism of the lower extremity complicated with myonephropathic metabolic syndrome(MMS).Methods: The extent of the welling and ischemia of the lower extremity,the change of creatine phosphokinase(CPK),serum glutamic-oxaloacetic transaminase(SGOT),lactat dehydrogeneise(LDH) and blood potassium were monitored.Creatinine BUN and pH of the blood were checked repeatedly.Once diagnosed,fasciotomy should be performed.In addition,alkalinization of the urine,oxygen-derived free radical scavengers should be administered.Results: Of the 48 patients with acute arterial embolism of the lower extremity,18 were complicated with MMS.One died,and the mortality rate was 11.1%;one case was amputated up the knee,and the rate of amputation was 5.6%.Conclusions: The rate of death or amputation might be reduced by embolectomy combined with the administration of vigorous hydration,alkalinization of the urine,oxygen-derived free radical scavengers and effective fasciotomy.
2005, 30(3): 230-232.
Abstract:
Objective: To study the efficiency of vascularized bone graft combined with reconstituted bone xeno graft(RBX) in repairing the bone defection and the expression of the vascular endothelial growth factor(VEGF) as an evaluation index of bone graft success.Methods: Twenty-seven cases of bone defection were randomly divided into vascularized bone graft-RBX group,vascularized bone graft group and RBX group.The clinical results were evaluated by the radiograph 3 months,6 months and 12 months after operation according to whether the bone defection was repaired,how long it took the bone to heal and whether the bone graft died away or not.The expression of VEGF in serum was assayed with immuno analysis method in the 3 groups 2 weeks,4 weeks,6 weeks and 8 weeks before and after the operation.Results: The bone defections were repaired in 8 cases,6 cases and 3 cases in the vascularized bone graft-RBX group,vascularized bone graft group and RBX group,respectively,3 months after the operation.One case,3 cases and 3 cases of bone defections in the vascularized bone graft-RBX group,vascularized bone graft group and RBX group achieved delayed recovery respectively 6 months after the operation.One case in the vascularized bone graft group and 2 cases in the RBX group developed part absorption in the bone transplantation area 12 months after the operation.The difference was not significant(P>0.05).Serum VEGF value was higher 2 weeks and 4 weeks after the operation than before the operation in all the 3 groups,which was more obvious with the vascularized bone graft-RBX group and vascularized bone graft group (P<0.01).The serum VEGF value 6 weeks and 8 weeks after the operation had no significant difference between the groups(P>0.05).Conclusions: Vascularized bone graft-RBX is more effective compared to the other 2 methods in repairing bone defections.After the operation,serum VEGF value in the vaschlarized bone graft-RBX group and the vascularized bone graft group increased,compared with that of the RBX group.The expression of serum VEGF increased obviously in the earlier period of bone transplantation.It is of value for clinical evaluation of the efficiency of bone defection repair with bone graft.
Objective: To study the efficiency of vascularized bone graft combined with reconstituted bone xeno graft(RBX) in repairing the bone defection and the expression of the vascular endothelial growth factor(VEGF) as an evaluation index of bone graft success.Methods: Twenty-seven cases of bone defection were randomly divided into vascularized bone graft-RBX group,vascularized bone graft group and RBX group.The clinical results were evaluated by the radiograph 3 months,6 months and 12 months after operation according to whether the bone defection was repaired,how long it took the bone to heal and whether the bone graft died away or not.The expression of VEGF in serum was assayed with immuno analysis method in the 3 groups 2 weeks,4 weeks,6 weeks and 8 weeks before and after the operation.Results: The bone defections were repaired in 8 cases,6 cases and 3 cases in the vascularized bone graft-RBX group,vascularized bone graft group and RBX group,respectively,3 months after the operation.One case,3 cases and 3 cases of bone defections in the vascularized bone graft-RBX group,vascularized bone graft group and RBX group achieved delayed recovery respectively 6 months after the operation.One case in the vascularized bone graft group and 2 cases in the RBX group developed part absorption in the bone transplantation area 12 months after the operation.The difference was not significant(P>0.05).Serum VEGF value was higher 2 weeks and 4 weeks after the operation than before the operation in all the 3 groups,which was more obvious with the vascularized bone graft-RBX group and vascularized bone graft group (P<0.01).The serum VEGF value 6 weeks and 8 weeks after the operation had no significant difference between the groups(P>0.05).Conclusions: Vascularized bone graft-RBX is more effective compared to the other 2 methods in repairing bone defections.After the operation,serum VEGF value in the vaschlarized bone graft-RBX group and the vascularized bone graft group increased,compared with that of the RBX group.The expression of serum VEGF increased obviously in the earlier period of bone transplantation.It is of value for clinical evaluation of the efficiency of bone defection repair with bone graft.
2005, 30(3): 233-234.
Abstract:
Objective: To evaluate the role of cardiac troponin I(cTnI) in determining the injury of cardiac muscle of the neonates with hypoxic-ischemic encephalopathy(HIE).Methods: The creatine kinase isoenzyme(CK-MB) and cTnI in the serum of 19 neonates with HIE were detected.Results: The levels of cTnI in the serum of the neonates with HIE were higher than normal neonates and there was marked different among the neonates with light,moderate and severe HIE (P<0.01).The levels of CK-MB had no significant difference in the serum of the neonates with light,moderate and severe HIE(P>0.05).Conclusions: The level of cTnI in serum is of great value in the diagnosis of the myocardial injury of neonates with HIE.
Objective: To evaluate the role of cardiac troponin I(cTnI) in determining the injury of cardiac muscle of the neonates with hypoxic-ischemic encephalopathy(HIE).Methods: The creatine kinase isoenzyme(CK-MB) and cTnI in the serum of 19 neonates with HIE were detected.Results: The levels of cTnI in the serum of the neonates with HIE were higher than normal neonates and there was marked different among the neonates with light,moderate and severe HIE (P<0.01).The levels of CK-MB had no significant difference in the serum of the neonates with light,moderate and severe HIE(P>0.05).Conclusions: The level of cTnI in serum is of great value in the diagnosis of the myocardial injury of neonates with HIE.
2005, 30(3): 235-236.
Abstract:
Objective: To explore the prevention of complications during the nasal endoscopic surgery.Methods: Sixty-four cases of sinusitis and nasal polyps underwent the nasal endoscopic surgery and received postsurgical cavity care and sinus washing.The complications were summarized.Results: The complications included periorbital ecchymosis(n=3),postoperative hemorrhage(n=1) and adhesion(n=5).The total incidence was 14%.No blindness or cerebrospinal fluid rhinorrhea.Conclusions: Familiarity with cranioverebral,nasal cavity and paranasal sinuses anatomy,detailed preoperational evaluation,perfect management during and after the operation can avoid complications in nasal endoscopic surgery.
Objective: To explore the prevention of complications during the nasal endoscopic surgery.Methods: Sixty-four cases of sinusitis and nasal polyps underwent the nasal endoscopic surgery and received postsurgical cavity care and sinus washing.The complications were summarized.Results: The complications included periorbital ecchymosis(n=3),postoperative hemorrhage(n=1) and adhesion(n=5).The total incidence was 14%.No blindness or cerebrospinal fluid rhinorrhea.Conclusions: Familiarity with cranioverebral,nasal cavity and paranasal sinuses anatomy,detailed preoperational evaluation,perfect management during and after the operation can avoid complications in nasal endoscopic surgery.
2005, 30(3): 237-239.
Abstract:
Objective: To investigate the effect and safety of nateglinide in treatment of patients with type 2 diabetes mellitus.Methods: A randomized double blind control trial was followed up for twelve weeks.Forty-eight selected cases were randomly divided into two groups in the ratio of one to one:observation group (nateglinide) and control group (repaglinide).The 2 hours plasma glucose(2hPG) and HbAlc levels of the patients were observed.Results: 2hPG and HbAlc levels of the two groups decreased obviously after treatment(P<0.01).The decreasing levels of 2hPG and HbAlc of each group' had no obvious differences (P>0.05).Conclusions: Nateglinide is effective and safe for the treatment of type 2 diabetes mellitus with slight side effects.
Objective: To investigate the effect and safety of nateglinide in treatment of patients with type 2 diabetes mellitus.Methods: A randomized double blind control trial was followed up for twelve weeks.Forty-eight selected cases were randomly divided into two groups in the ratio of one to one:observation group (nateglinide) and control group (repaglinide).The 2 hours plasma glucose(2hPG) and HbAlc levels of the patients were observed.Results: 2hPG and HbAlc levels of the two groups decreased obviously after treatment(P<0.01).The decreasing levels of 2hPG and HbAlc of each group' had no obvious differences (P>0.05).Conclusions: Nateglinide is effective and safe for the treatment of type 2 diabetes mellitus with slight side effects.
2005, 30(3): 239-240.
Abstract:
Objective: To improve the therapeutic results for treatment of trigeminal neuralgia Ⅱ and to reduce recurrences.Methods: Sixty-five patients with second trigeminal neuralgia Ⅱ with avulsion of nerve and tissue were treated with microwave thermocoagulation.Results: All the patients were followed up for 2 to 5 years and the total response rate was 100% in recent years.Complete remission was found in 80% of the patients(52/65)and recurrence was found in 20%(13/65).Conclusions: The method proves to be effective in treating primary trigeminal neuralgia.It has the advantages of safety,slight injury,quick recovery and low recurrence rate.
Objective: To improve the therapeutic results for treatment of trigeminal neuralgia Ⅱ and to reduce recurrences.Methods: Sixty-five patients with second trigeminal neuralgia Ⅱ with avulsion of nerve and tissue were treated with microwave thermocoagulation.Results: All the patients were followed up for 2 to 5 years and the total response rate was 100% in recent years.Complete remission was found in 80% of the patients(52/65)and recurrence was found in 20%(13/65).Conclusions: The method proves to be effective in treating primary trigeminal neuralgia.It has the advantages of safety,slight injury,quick recovery and low recurrence rate.
2005, 30(3): 241-243.
Abstract:
Objective: To evaluate the effect of 89SrCl therapy on patients with metastatic carcinoma of bone.Methods: Twenty-two patients were treated by intravenous 89SrCl at a dose of 1.48-2.22 MBq/kg.The bone-pain,lesion and the side-effect were observed.The imaging and serum tumor makers were followed up.Results: The bone-pain vanished or eased in 18 cases after the therapy.The response rate was 81.8%.The lesions became small or vanished in some patients and the serum tumor markers and alkaline phosphates(AKP) came down.Conclusions: 89SrCl has obvious effect on patients with metastatic carcinoma of bone and the side-effect is mild.
Objective: To evaluate the effect of 89SrCl therapy on patients with metastatic carcinoma of bone.Methods: Twenty-two patients were treated by intravenous 89SrCl at a dose of 1.48-2.22 MBq/kg.The bone-pain,lesion and the side-effect were observed.The imaging and serum tumor makers were followed up.Results: The bone-pain vanished or eased in 18 cases after the therapy.The response rate was 81.8%.The lesions became small or vanished in some patients and the serum tumor markers and alkaline phosphates(AKP) came down.Conclusions: 89SrCl has obvious effect on patients with metastatic carcinoma of bone and the side-effect is mild.
2005, 30(3): 243-244.
Abstract:
Objective: To discuss the clinical features of allergic purpura.Methods: Seventy-three patients with allergic purpura were analyzed retrospectively.Results: Of the 73 cases,47% of the allergic purpura occurred in February,March and April,and 30% in October,November and December.36% of the patients had a history of upper respiratory tract infection in the early days.47% of the cases presented abdominal pain,29% hematochezia,33% joint pain,14% hematuria and 34% proteinuria.The serum level of ALT was increased in 8% of patients and change of ECG was observed in 8% of the patients.Conclusions: Allergic purpura occurs more frequently in the spring and winter.Upper respiratory tract infection may be the common risk factor for the reoccurrence of allergic purpura,so treatment of upper respiratory tract infection should be emphasized.Gastrointestinal purpura with abdominal pain ahead of purpura should be distinguished from acute abdomen deceases.Arthritic purpura was often involved with symptoms of the knees or the ankles.Nephritis purpura was usually associated with proteinuria or hematuria,so routine urine detection should be conducted.
Objective: To discuss the clinical features of allergic purpura.Methods: Seventy-three patients with allergic purpura were analyzed retrospectively.Results: Of the 73 cases,47% of the allergic purpura occurred in February,March and April,and 30% in October,November and December.36% of the patients had a history of upper respiratory tract infection in the early days.47% of the cases presented abdominal pain,29% hematochezia,33% joint pain,14% hematuria and 34% proteinuria.The serum level of ALT was increased in 8% of patients and change of ECG was observed in 8% of the patients.Conclusions: Allergic purpura occurs more frequently in the spring and winter.Upper respiratory tract infection may be the common risk factor for the reoccurrence of allergic purpura,so treatment of upper respiratory tract infection should be emphasized.Gastrointestinal purpura with abdominal pain ahead of purpura should be distinguished from acute abdomen deceases.Arthritic purpura was often involved with symptoms of the knees or the ankles.Nephritis purpura was usually associated with proteinuria or hematuria,so routine urine detection should be conducted.
2005, 30(3): 245-246.
Abstract:
Objective: To introduce the experience in the microsurgical removal of giant pituitary adenomas through three approaches.Methods: Thirty-six cases of giant pituitary adenomas were treated by microsurgery.The giant pituitary adenomas were excised through subfrontal approach in 22 cases,pterion approach in 13 cases,combined approach in 1 case and nasal cavity-sphenoidal sinus approach in 1 case.Results: Of the 36 patients,26 achieved total removal of the tumors,8 subtotal removal and 2 part removal.After the operation,the eyesight was obviously improved in 30 cases.Complications occurred in 10 cases,among whom,4 suffered from diabetes insipidus,2 suffered from bleeding in stomach and the right eyesight in 4 cases got worse,but after 10 days,the eyesight revived.One case died.The fellow-up of 6 months showed that the symptoms were improved in 35 cases.Conclusions: Microsurgery by the subfrontal,pterional or combined approach is effective for the treatment of giant pituitary adenomas with a high total removal rate.
Objective: To introduce the experience in the microsurgical removal of giant pituitary adenomas through three approaches.Methods: Thirty-six cases of giant pituitary adenomas were treated by microsurgery.The giant pituitary adenomas were excised through subfrontal approach in 22 cases,pterion approach in 13 cases,combined approach in 1 case and nasal cavity-sphenoidal sinus approach in 1 case.Results: Of the 36 patients,26 achieved total removal of the tumors,8 subtotal removal and 2 part removal.After the operation,the eyesight was obviously improved in 30 cases.Complications occurred in 10 cases,among whom,4 suffered from diabetes insipidus,2 suffered from bleeding in stomach and the right eyesight in 4 cases got worse,but after 10 days,the eyesight revived.One case died.The fellow-up of 6 months showed that the symptoms were improved in 35 cases.Conclusions: Microsurgery by the subfrontal,pterional or combined approach is effective for the treatment of giant pituitary adenomas with a high total removal rate.
2005, 30(3): 247-249.
Abstract:
Objective: To explore the clinical efficiency and safety of pazufloxacin sodium chloride injection in treatment of lower respiratory infection.Methods: The clinical effect of pazufloxacin on lower respiratory infection was observed and compared with that of levofloxacin.Thirty patients were randomly divided into treatment group(16 cases) and control group(14 cases) receiving pazufloxacin and levofloxacin,respectively,for 7 to 14 days.Results: Pazufloxacin and levofloxacin were used for (8.74±2.28) days and(8.47±2.05) days,respectively(P>0.05).The total response rates were 93.75% and 92.86% in the treatment group and control group,respectively(P>0.05).There was no side difference in common data between the two groups.During the course of treatment,no side effect related to the drug occurred.Conclusions: It is safe and effective to treat lower respiratory infection with pazufloxacin sodium chloride injection.
Objective: To explore the clinical efficiency and safety of pazufloxacin sodium chloride injection in treatment of lower respiratory infection.Methods: The clinical effect of pazufloxacin on lower respiratory infection was observed and compared with that of levofloxacin.Thirty patients were randomly divided into treatment group(16 cases) and control group(14 cases) receiving pazufloxacin and levofloxacin,respectively,for 7 to 14 days.Results: Pazufloxacin and levofloxacin were used for (8.74±2.28) days and(8.47±2.05) days,respectively(P>0.05).The total response rates were 93.75% and 92.86% in the treatment group and control group,respectively(P>0.05).There was no side difference in common data between the two groups.During the course of treatment,no side effect related to the drug occurred.Conclusions: It is safe and effective to treat lower respiratory infection with pazufloxacin sodium chloride injection.
2005, 30(3): 249-250.
Abstract:
Objective: To evaluate the effect and safety of oxaliplatin(OXA) combined with calcium folinate(CF) and fluorouracil(5-Fu) regimen for patients with advanced gastric cancer.Methods: OXA 130 mg/m2 (2 hours infusion on the first day),CF 200 mg/m2 (1 hour infusion for 5 days) and 5-Fu 350 mg/m2(4 hours infusion on the first day) were administered and the regimen was repeated every 3 weeks.The efficacy was evaluated after two repeated cycles were completed.Results: Twenty-eight patients were included in the study.One patient achieved CR,11 PR,10 SD and 6 PD.The overall response rate was 42.9%.The main adverse effects were bone marrow suppression,nausea,vomiting and neurosensory toxicity.Conclusions: OXA-CF-5-Fu regimen is highly effective for treatment of advanced gastric cancer with mild toxicity.
Objective: To evaluate the effect and safety of oxaliplatin(OXA) combined with calcium folinate(CF) and fluorouracil(5-Fu) regimen for patients with advanced gastric cancer.Methods: OXA 130 mg/m2 (2 hours infusion on the first day),CF 200 mg/m2 (1 hour infusion for 5 days) and 5-Fu 350 mg/m2(4 hours infusion on the first day) were administered and the regimen was repeated every 3 weeks.The efficacy was evaluated after two repeated cycles were completed.Results: Twenty-eight patients were included in the study.One patient achieved CR,11 PR,10 SD and 6 PD.The overall response rate was 42.9%.The main adverse effects were bone marrow suppression,nausea,vomiting and neurosensory toxicity.Conclusions: OXA-CF-5-Fu regimen is highly effective for treatment of advanced gastric cancer with mild toxicity.
2005, 30(3): 251-253.
Abstract:
Objective: To observe the effect of dynamic hip screw(DHS) in the treatment of intertrochanteric fractures of femur in the elderly.Methods: Forty-five elderly patients with intertrochanteric fractures of femur treated by DHS from Jan.2001 to Jun.2003 in our hospital were studied.The fracture type,surgical procedure,preexistent diseases before operation,treatment after operation and curative effect were summarized.Results: One case died of pneumonia and heart failure;the other 44 cases were followed up for an average of 21.6 months.The results were excellent in 84.1% cases,good in 11.4% cases and poor in 4.5% cases.Conclusions: DHS internal fixation is an ideal method for treatment of intertrochanteric fractures of femur in the elderly with the advantages of strong fixation,lower morbidity and mortality,and early recovery of motion.
Objective: To observe the effect of dynamic hip screw(DHS) in the treatment of intertrochanteric fractures of femur in the elderly.Methods: Forty-five elderly patients with intertrochanteric fractures of femur treated by DHS from Jan.2001 to Jun.2003 in our hospital were studied.The fracture type,surgical procedure,preexistent diseases before operation,treatment after operation and curative effect were summarized.Results: One case died of pneumonia and heart failure;the other 44 cases were followed up for an average of 21.6 months.The results were excellent in 84.1% cases,good in 11.4% cases and poor in 4.5% cases.Conclusions: DHS internal fixation is an ideal method for treatment of intertrochanteric fractures of femur in the elderly with the advantages of strong fixation,lower morbidity and mortality,and early recovery of motion.
2005, 30(3): 253-254.
Abstract:
Objective: To investigate the clinical characteristics,pathological features and surgical indications of polypoid lesions of gallbladder(PLG).Methods: One hundred and four cases of PLG confirmed by pathological examination were analyzed retrospectively.Results: PLG were more frequently found in middle aged people.They presented no special clinical features.Cholesterol PLG was the most common PLG.Most Cholesterol PLG was multiple foci with a mean diameter of <5 mm,while adenomas PLG presented single focus with a mean diameter of >10 mm.Conclusions: The patients older than 50 years with single polyp lesion or complicated with gallbladder stone should resort to surgical treatment.Patients with obvious clinical symptoms which have influenced their normal lives should receive surgical intervention.Patients with no symptoms should be followed up periodically.
Objective: To investigate the clinical characteristics,pathological features and surgical indications of polypoid lesions of gallbladder(PLG).Methods: One hundred and four cases of PLG confirmed by pathological examination were analyzed retrospectively.Results: PLG were more frequently found in middle aged people.They presented no special clinical features.Cholesterol PLG was the most common PLG.Most Cholesterol PLG was multiple foci with a mean diameter of <5 mm,while adenomas PLG presented single focus with a mean diameter of >10 mm.Conclusions: The patients older than 50 years with single polyp lesion or complicated with gallbladder stone should resort to surgical treatment.Patients with obvious clinical symptoms which have influenced their normal lives should receive surgical intervention.Patients with no symptoms should be followed up periodically.
2005, 30(3): 255-256.
Abstract:
Objective: To investigate the clinical features and management of the peri-operative period of laparoscopic cholecystectomy in the elderly.Methods: Two hundred eighty patients who were more than 65 years of age and had received laparoscopic cholecystectomy were analyzed retrospectively.The average duration of operation,average hospital stay,the number of cases changing to laparotomy,the complications and the changes of pneumonary function and blood gas were compared with those of the young patients receiving laparoscopic cholecystectomy and the aged patients performed laparotomy.Results: For the old patients receiving cholecystectomy,the average duration of operation was 38 minutes;the rate of changing to laparotomy was 1.1%;and the complication rate was 0.4%,which was higher than that of the young patients but lower than that of the aged patients receiving laparotomy(P<0.01).The occurrence of pneumonia dysfunction and hypercapnia(20.0%) in the patients treated by cholecystectomy was obviously higher than those by laparotomy (P<0.01),but most can recover quickly after operation.Conclusions: Laparoscopic cholecystectomy has the advantages of little injury and fast recovery for the aged patients.Rational and careful management in the peri-operative period can help the old patients withstand the laparoscopic cholecystectomy.
Objective: To investigate the clinical features and management of the peri-operative period of laparoscopic cholecystectomy in the elderly.Methods: Two hundred eighty patients who were more than 65 years of age and had received laparoscopic cholecystectomy were analyzed retrospectively.The average duration of operation,average hospital stay,the number of cases changing to laparotomy,the complications and the changes of pneumonary function and blood gas were compared with those of the young patients receiving laparoscopic cholecystectomy and the aged patients performed laparotomy.Results: For the old patients receiving cholecystectomy,the average duration of operation was 38 minutes;the rate of changing to laparotomy was 1.1%;and the complication rate was 0.4%,which was higher than that of the young patients but lower than that of the aged patients receiving laparotomy(P<0.01).The occurrence of pneumonia dysfunction and hypercapnia(20.0%) in the patients treated by cholecystectomy was obviously higher than those by laparotomy (P<0.01),but most can recover quickly after operation.Conclusions: Laparoscopic cholecystectomy has the advantages of little injury and fast recovery for the aged patients.Rational and careful management in the peri-operative period can help the old patients withstand the laparoscopic cholecystectomy.
2005, 30(3): 257-259.
Abstract:
Objective: To evaluate and compare the efficacy of ningmitai-terazosin and prostat in treatment of chronic nonbacterial prostatitis(CNP).Methods: Based on National Institutes of Health(NIH) classification,118 cases of CNP were randomly divided into ningmitai-terazosin group(60 cases) and prostat group(58 cases).The drugs were administered for 8 weeks and the clinical symptoms before and after treatment were surveyed.The chronic prostatitis symptom index(CPSI) of each patient was evaluated.Results: After 8 weeks' treatment,the symptoms of the two groups improved significantly(P<0.01).The difference in CNP was not significant between the two groups(P>0.05).Compared with pretreatment,the difference in the reduction of micturition scores and ache scores was significant between the two groups(P<0.01).Conclusions: Both ningmitai-terazosin and prostat have a positive effect on CNP.Ningmitai-terazosin is more effective in relieving the stimulus of urethra,while prostat can rapidly relieve the pelvic pain.
Objective: To evaluate and compare the efficacy of ningmitai-terazosin and prostat in treatment of chronic nonbacterial prostatitis(CNP).Methods: Based on National Institutes of Health(NIH) classification,118 cases of CNP were randomly divided into ningmitai-terazosin group(60 cases) and prostat group(58 cases).The drugs were administered for 8 weeks and the clinical symptoms before and after treatment were surveyed.The chronic prostatitis symptom index(CPSI) of each patient was evaluated.Results: After 8 weeks' treatment,the symptoms of the two groups improved significantly(P<0.01).The difference in CNP was not significant between the two groups(P>0.05).Compared with pretreatment,the difference in the reduction of micturition scores and ache scores was significant between the two groups(P<0.01).Conclusions: Both ningmitai-terazosin and prostat have a positive effect on CNP.Ningmitai-terazosin is more effective in relieving the stimulus of urethra,while prostat can rapidly relieve the pelvic pain.
2005, 30(3): 259-260.
Abstract:
Objective: To study the efficacy and mechanism of high does intravenous immunoglobulin(HDIVIG) in therapeutic of idiopathic thrombopenic purpura(ITP).Methods: Forty eight cases of ITP were divided into therapeutic group and control group according to the time of admission to the hospital.They were all given hemostatic and adrenal cortex hormone.The therapeutic group was administered HDIVIG 0.4 g·kg-1·d-1 for five days continously.Results: The number of blood platelet of 76.19% cases in the treatment group rose to the normal level on the 5th day of therapeutic.The Blood platelet count of the therapeutic group rose more rapidly comparing with the control group(P<0.01).Conclusions: Treatment of ITP with HDIVIG can raise the number of blood platelet quickly and shorten the hospital stay.Joint usage of adrenal contex hormone can reduce recurrence.
Objective: To study the efficacy and mechanism of high does intravenous immunoglobulin(HDIVIG) in therapeutic of idiopathic thrombopenic purpura(ITP).Methods: Forty eight cases of ITP were divided into therapeutic group and control group according to the time of admission to the hospital.They were all given hemostatic and adrenal cortex hormone.The therapeutic group was administered HDIVIG 0.4 g·kg-1·d-1 for five days continously.Results: The number of blood platelet of 76.19% cases in the treatment group rose to the normal level on the 5th day of therapeutic.The Blood platelet count of the therapeutic group rose more rapidly comparing with the control group(P<0.01).Conclusions: Treatment of ITP with HDIVIG can raise the number of blood platelet quickly and shorten the hospital stay.Joint usage of adrenal contex hormone can reduce recurrence.
2005, 30(3): 267-269.
Abstract:
Objective: To investigate the development of protein-energy malnutrition in patients with chronic renal failure in early stage and provide measures of prevention.Methods: The clinical data of 40 cases with chronic renal failure in early stage were observed.Results: The serum albumin was <40 g/L in 60.00% of the 40 cases,the serum cholesterol was <3.9mmol/L in 37.50% and the Hb was <110 g/L in 66.70%.Conclusions: Lack of nutrition occurred in early stage of chronic renal failure.Evaluating the nutrition of patients with chronic renal failure in early stage should be emphasized and measures should be taken to prevent it.
Objective: To investigate the development of protein-energy malnutrition in patients with chronic renal failure in early stage and provide measures of prevention.Methods: The clinical data of 40 cases with chronic renal failure in early stage were observed.Results: The serum albumin was <40 g/L in 60.00% of the 40 cases,the serum cholesterol was <3.9mmol/L in 37.50% and the Hb was <110 g/L in 66.70%.Conclusions: Lack of nutrition occurred in early stage of chronic renal failure.Evaluating the nutrition of patients with chronic renal failure in early stage should be emphasized and measures should be taken to prevent it.
2005, 30(3): 269-270.
Abstract:
Objective: To explore the influence of urination training in bed before percutaneous coronary intervention(PCI) on the occurrence of uroschesis and hemorrhage in the puncture site after PCI.Methods: One hundred and two patients were randomly divided into observation group(n=52) and control group(n=50).The observation group were trained to urinate in bed before PCI,and the occurrence of uroschesis and hemorrhage in puncture site after PCI were compared.Results: The occurrence of uroschesis in the observation group was significantly lower than that of the control group(P<0.01),and the occurrence of hemorrhage in puncture site in observation group was also lower than that of the control group(P<0.05).Conclusions: This study shows that urination training in bed before PCI can effectively decrease the occurrence of uroschesis and hemorrhage in puncture site after PCI.
Objective: To explore the influence of urination training in bed before percutaneous coronary intervention(PCI) on the occurrence of uroschesis and hemorrhage in the puncture site after PCI.Methods: One hundred and two patients were randomly divided into observation group(n=52) and control group(n=50).The observation group were trained to urinate in bed before PCI,and the occurrence of uroschesis and hemorrhage in puncture site after PCI were compared.Results: The occurrence of uroschesis in the observation group was significantly lower than that of the control group(P<0.01),and the occurrence of hemorrhage in puncture site in observation group was also lower than that of the control group(P<0.05).Conclusions: This study shows that urination training in bed before PCI can effectively decrease the occurrence of uroschesis and hemorrhage in puncture site after PCI.
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