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2006 Vol. 31, No. 1
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2006, 31(1): 1-4.
Abstract:
Objective: To establish a method for detection of NF-κB activity in human T cells after activation using gelelectrophorestic mobility shift assay(EMSA) and enhanced chemiluminescent(ECL) technique.Methods: Human peripheral blood mononuclear cells(PBMC) were stimulated with anti-CD3 mAb to generate activated T cells.Cell nuclear protein extracts were mixed with biotin-label probe of NF-κB specific oligonucleotides sequence,and protein-probe complex were separated from free probe by electrophoresis in nondenaturing polyacrylamide gel,and electrotransferred to positive charged nylon membrane,and added with streptoavidin-HRP conjugate and enhanced chemiluminescent reagent to detect NF-κB activity.Results: The NF-κB shift band were detected at 15 min after stimulation of human PBMC,and reached maximum peak at 30 min,and then decreased at 60 min of stimulation,and the results of control group demonstrated the method with high specific and sensitivity.Conclusions: Enhanced chemiluminescence based EMSA is very convenient and high sensitive technique to detect nuclear factor such as NF-κB activity in T cells after activation.
Objective: To establish a method for detection of NF-κB activity in human T cells after activation using gelelectrophorestic mobility shift assay(EMSA) and enhanced chemiluminescent(ECL) technique.Methods: Human peripheral blood mononuclear cells(PBMC) were stimulated with anti-CD3 mAb to generate activated T cells.Cell nuclear protein extracts were mixed with biotin-label probe of NF-κB specific oligonucleotides sequence,and protein-probe complex were separated from free probe by electrophoresis in nondenaturing polyacrylamide gel,and electrotransferred to positive charged nylon membrane,and added with streptoavidin-HRP conjugate and enhanced chemiluminescent reagent to detect NF-κB activity.Results: The NF-κB shift band were detected at 15 min after stimulation of human PBMC,and reached maximum peak at 30 min,and then decreased at 60 min of stimulation,and the results of control group demonstrated the method with high specific and sensitivity.Conclusions: Enhanced chemiluminescence based EMSA is very convenient and high sensitive technique to detect nuclear factor such as NF-κB activity in T cells after activation.
2006, 31(1): 4-6.
Abstract:
Objective: To construct expression vector of the metalloproteinase domain of human tumor necrosis factor-α converting enzyme(TACEmp) and express its protein in E.coli.Methods: RT-PCR and sequencing were used to clone and confirm the TACEmp gene.The fusion expression vector was constructed with the prokaryotic expression vector including disulfide isomerase dsbA gene and the TACEmp,named pET-DsbAmut-TACEmp,and transformed into E.coli.BL21.After induced by IPTG,expression product was analyzed by SDS-PAGE.Results: TACEmp was cloned by RT-PCR.The recombinant vector pET-DsbAmut-TACEmp was constructed.The fusion protein was expressed at high level in E.coli.Conclusions: The TACEmp was expressed at high level in prokaryotic expression system by using fusion protein technology.The expression of TACEmp protein may be useful for the study of biological functions of TACEmp and it's biotherapy in TACEmp related diseases.
Objective: To construct expression vector of the metalloproteinase domain of human tumor necrosis factor-α converting enzyme(TACEmp) and express its protein in E.coli.Methods: RT-PCR and sequencing were used to clone and confirm the TACEmp gene.The fusion expression vector was constructed with the prokaryotic expression vector including disulfide isomerase dsbA gene and the TACEmp,named pET-DsbAmut-TACEmp,and transformed into E.coli.BL21.After induced by IPTG,expression product was analyzed by SDS-PAGE.Results: TACEmp was cloned by RT-PCR.The recombinant vector pET-DsbAmut-TACEmp was constructed.The fusion protein was expressed at high level in E.coli.Conclusions: The TACEmp was expressed at high level in prokaryotic expression system by using fusion protein technology.The expression of TACEmp protein may be useful for the study of biological functions of TACEmp and it's biotherapy in TACEmp related diseases.
2006, 31(1): 7-8.
Abstract:
Objective: To elucidate the changes of nitric oxide production in cerebral cortex of rats under acute cold stress by immersing in water.Methods: Twenty-seven male adult rats were randomly divided into three groups.Group A was a control group and rats in which were without any treatment.Rats of group B were put into cold water for 30 minutes and rats of group C were put into cold water for 60 minutes.The level of nitric oxide in cortex of rats were deteated by using NO deteat Kit.Results: The levels of nitric oxide in group A,B,and C were(4.36±1.79) mmol/g pr;(2.79±2.00) mmol/gpr,and(11.50±2.34) mmol/gpr,respectively.Conclusions: Nitric oxide may play a protective role in the early stage of acute cold stress.
Objective: To elucidate the changes of nitric oxide production in cerebral cortex of rats under acute cold stress by immersing in water.Methods: Twenty-seven male adult rats were randomly divided into three groups.Group A was a control group and rats in which were without any treatment.Rats of group B were put into cold water for 30 minutes and rats of group C were put into cold water for 60 minutes.The level of nitric oxide in cortex of rats were deteated by using NO deteat Kit.Results: The levels of nitric oxide in group A,B,and C were(4.36±1.79) mmol/g pr;(2.79±2.00) mmol/gpr,and(11.50±2.34) mmol/gpr,respectively.Conclusions: Nitric oxide may play a protective role in the early stage of acute cold stress.
2006, 31(1): 9-12.
Abstract:
Objective: To investigate the expression of cyclooxygenase-2(COX-2) and p53 in breast carcinoma,and to evaluate its relation to microvascular density and lymph node metastasis.Methods: S-P immunohistochemistry and in situ hybridization assay were utilized to measure the protein and mRNA expression of COX-2 in 60 breast carcinoma and 20 adjacent normal tissues.The Ⅷ-RAg,p53 expression were measure by S-P immunohistochemical method,and the density of microvessel in the breast carcinoma was assayed.Results: The microvascular density(MVD) in COX-2(+) or p53(+) group was significantly higher than that in COX-2(-) or p53(-) group(P<0.01).The MVD reached the maximum in the patients whose COX-2 and p53 were both positive(P<0.01).The positive rates of p53 and COX-2 in cases with lymph node metastasis were both significantly higher than that in the cases without lymph node metastasis,respectively(P<0.05 and P<0.05).Conclusions: COX-2 and p53 are related to tumor angiogenesis and lymph node metastasis in breast carcinoma.COX-2 and p53 show a positive correlation in breast carcinoma angiogenesis.
Objective: To investigate the expression of cyclooxygenase-2(COX-2) and p53 in breast carcinoma,and to evaluate its relation to microvascular density and lymph node metastasis.Methods: S-P immunohistochemistry and in situ hybridization assay were utilized to measure the protein and mRNA expression of COX-2 in 60 breast carcinoma and 20 adjacent normal tissues.The Ⅷ-RAg,p53 expression were measure by S-P immunohistochemical method,and the density of microvessel in the breast carcinoma was assayed.Results: The microvascular density(MVD) in COX-2(+) or p53(+) group was significantly higher than that in COX-2(-) or p53(-) group(P<0.01).The MVD reached the maximum in the patients whose COX-2 and p53 were both positive(P<0.01).The positive rates of p53 and COX-2 in cases with lymph node metastasis were both significantly higher than that in the cases without lymph node metastasis,respectively(P<0.05 and P<0.05).Conclusions: COX-2 and p53 are related to tumor angiogenesis and lymph node metastasis in breast carcinoma.COX-2 and p53 show a positive correlation in breast carcinoma angiogenesis.
2006, 31(1): 12-14.
Abstract:
Objective: To find a method of evaluating anti-Mycobacterium tuberculosis(TB)medicine using medicine containing serum and infected macrophage model and investigate its practicability and merits.Methods: By using the culture system constituting with serum containing isoniazid and infected macrophages,the numbers of the survival TB were confirmed through the clones of germiculture after these cells were cultured with serum containing isoniazid for several days,then the isoniazid's activity in this culture system was reflected and compared with the MIC.Results: The survival TB clones in the groups of low dose and high dose of isoniazid containing serum were remarkably fewer than those in the control(P<0.05-P<0.01).Conclusions: The medicine's function of anti-Mycobacterium tuberculosis can be objectively evaluated by using medicine containing serum and infected macrophage model.
Objective: To find a method of evaluating anti-Mycobacterium tuberculosis(TB)medicine using medicine containing serum and infected macrophage model and investigate its practicability and merits.Methods: By using the culture system constituting with serum containing isoniazid and infected macrophages,the numbers of the survival TB were confirmed through the clones of germiculture after these cells were cultured with serum containing isoniazid for several days,then the isoniazid's activity in this culture system was reflected and compared with the MIC.Results: The survival TB clones in the groups of low dose and high dose of isoniazid containing serum were remarkably fewer than those in the control(P<0.05-P<0.01).Conclusions: The medicine's function of anti-Mycobacterium tuberculosis can be objectively evaluated by using medicine containing serum and infected macrophage model.
Effect of dopamine on diaphragmatic fatigue in vitro induced by hypoxic hypercapnic acidosis in rats
2006, 31(1): 15-17.
Abstract:
Objective: To observe the treatment effect of dopamine on diaphragmatic fatigue in vitro induced by hypoxic hypercapnic acidosis in rats.Methods: Thirty-two SD rats were divided into four groups at random:control group,control group with dopamine,model group,model group with dopamine.Control group and control group with dopamine were aerated oxygen(95%O2+5%CO2),model group and model group with dopamine were aerated hypoxic hypercapnic acidosis gas(21%O2,12%CO2,67%N2) for 4 min.At the same time dopamine (1 mmol/L) was added to the second and forth groups.Data(Pt,Po,CT,1/2RT) was recorded and analyzed using the Medlab-U/4c biological signal collecting system.Results: Compared with control group,the Pt,Po and contractile force under different frequency of model group decreased significantly(P<0.05 and P<0.01);but the Po and contractile force under the frequencies 30-100 Hz of dopamine increased significantly(P<0.05) compared with those of model group.Conclusions: Dopamine can improve contractility and treatment of high frequency fatigue induced by hypoxic hypercapnic acidosis to some extent.
Objective: To observe the treatment effect of dopamine on diaphragmatic fatigue in vitro induced by hypoxic hypercapnic acidosis in rats.Methods: Thirty-two SD rats were divided into four groups at random:control group,control group with dopamine,model group,model group with dopamine.Control group and control group with dopamine were aerated oxygen(95%O2+5%CO2),model group and model group with dopamine were aerated hypoxic hypercapnic acidosis gas(21%O2,12%CO2,67%N2) for 4 min.At the same time dopamine (1 mmol/L) was added to the second and forth groups.Data(Pt,Po,CT,1/2RT) was recorded and analyzed using the Medlab-U/4c biological signal collecting system.Results: Compared with control group,the Pt,Po and contractile force under different frequency of model group decreased significantly(P<0.05 and P<0.01);but the Po and contractile force under the frequencies 30-100 Hz of dopamine increased significantly(P<0.05) compared with those of model group.Conclusions: Dopamine can improve contractility and treatment of high frequency fatigue induced by hypoxic hypercapnic acidosis to some extent.
2006, 31(1): 17-19.
Abstract:
Objective: To observe the role of acanthopanax senticosus injection in the protection of acute myocardial anoxia in mice.Methods: The mean survival time and the capacity of myocardial oxygen consumption under ordinary pressure in mice were observed and the disappearance time of electrocardiographic sign in mice was measured by clamping their trache.Results: Acanthopanax senticosus injection of various dosages could prolong the mean survival time and decrease the disappearance time of electrocardiographic sign in mice.Conclusions: Acanthopanax senticosus injection can evidently reduce the capacity of myocardial oxygen consumption,protect the hypoxic myocardium and increase the ability of anti-stress in mice with acute myocardial anoxia.
Objective: To observe the role of acanthopanax senticosus injection in the protection of acute myocardial anoxia in mice.Methods: The mean survival time and the capacity of myocardial oxygen consumption under ordinary pressure in mice were observed and the disappearance time of electrocardiographic sign in mice was measured by clamping their trache.Results: Acanthopanax senticosus injection of various dosages could prolong the mean survival time and decrease the disappearance time of electrocardiographic sign in mice.Conclusions: Acanthopanax senticosus injection can evidently reduce the capacity of myocardial oxygen consumption,protect the hypoxic myocardium and increase the ability of anti-stress in mice with acute myocardial anoxia.
2006, 31(1): 20-23.
Abstract:
Objective: To study the relationship between plasma level of vascular endothelial growth factor(VEGF) and clinicopathological parameters and prognosis in esophageal squamous cell carcinoma(ESCC).Methods: ELISA was used to examine the plasma level of VEGF in 86 patients with ESCC.The clinicopathological parameters of every patient were recorded.Results: The patients were divided into two groups according to the degree of lymph nodes resection.The average number of resected lymph nodes and the average time of operation were significantly different between the extend lymph node dissection group and the esophagectomy group(P<0.01),but the difference was not significant in the occurrence of complications(P>0.05).In the esophagectomy group,l year relapse or metastasis rate in patients with plasma VEGF positive was significantly higher than that with negative ones(P<0.05).In the extent lymph node resection group,l year relapse or metastasis rate,number of positive lymph node in plasma VEGF positive patients were significant higher than that of plasma VEGF negative patients(P<0.01).Conclusions: Plasma VEGF is significantly related to the clinicopathological parameter and prognosis of ESCC,and can provide valuable reference to the choice of operation and adjuvant therapy.
Objective: To study the relationship between plasma level of vascular endothelial growth factor(VEGF) and clinicopathological parameters and prognosis in esophageal squamous cell carcinoma(ESCC).Methods: ELISA was used to examine the plasma level of VEGF in 86 patients with ESCC.The clinicopathological parameters of every patient were recorded.Results: The patients were divided into two groups according to the degree of lymph nodes resection.The average number of resected lymph nodes and the average time of operation were significantly different between the extend lymph node dissection group and the esophagectomy group(P<0.01),but the difference was not significant in the occurrence of complications(P>0.05).In the esophagectomy group,l year relapse or metastasis rate in patients with plasma VEGF positive was significantly higher than that with negative ones(P<0.05).In the extent lymph node resection group,l year relapse or metastasis rate,number of positive lymph node in plasma VEGF positive patients were significant higher than that of plasma VEGF negative patients(P<0.01).Conclusions: Plasma VEGF is significantly related to the clinicopathological parameter and prognosis of ESCC,and can provide valuable reference to the choice of operation and adjuvant therapy.
2006, 31(1): 23-24.
Abstract:
Objective: To observe the curative effect of 1% sodium hyaluronate(SH) injection in the treatment of osteoarthritis of the knee.Methods: SH injection was give 2 ml five times at intervals of one week through routine knee joint puncture,and compare Solu-Medrol sterile Powder(MP) injection was give 40 mg three times used the same ways.Results: The total effective rate was 94.2% after five weeks of etreatment in SH group.There was no recurrence of this disease in 88.4% of the patients 12 months after treatment.And in MP group,the total effective rate was 91.9%,83.8% of the patients who had been cured didn't recurrent after 12 months.Two groups is no significant difference(P>0.05).Conclusions: SH injection is an effective drug for treatment of osteoarthritis of the knee.Its tenderness relief and no poision or less side effect.
Objective: To observe the curative effect of 1% sodium hyaluronate(SH) injection in the treatment of osteoarthritis of the knee.Methods: SH injection was give 2 ml five times at intervals of one week through routine knee joint puncture,and compare Solu-Medrol sterile Powder(MP) injection was give 40 mg three times used the same ways.Results: The total effective rate was 94.2% after five weeks of etreatment in SH group.There was no recurrence of this disease in 88.4% of the patients 12 months after treatment.And in MP group,the total effective rate was 91.9%,83.8% of the patients who had been cured didn't recurrent after 12 months.Two groups is no significant difference(P>0.05).Conclusions: SH injection is an effective drug for treatment of osteoarthritis of the knee.Its tenderness relief and no poision or less side effect.
2006, 31(1): 25-26.
Abstract:
Objective: To observe the therapatic effect of intravesical instillation of hydroxycamptothecin(HCPT) combining transurethral electrovaporization in the treatment of cystitis glandularis.Methods: Twenty-four patients with cystitis glandularis were removed of the inducements,and then treated with intravesical instillation of HCPT after transurethral resection.Results: All the 24 cases were followed up.The recurrence was observed in 2 cases.The clinical symptoms of 18 cases disappeared and 4 were alleviated.Conclusions: Combination of the transurethral electrovaporization with HCPT intravesical instillation is an effective method in treating local recurrence of cystitis glandularis.
Objective: To observe the therapatic effect of intravesical instillation of hydroxycamptothecin(HCPT) combining transurethral electrovaporization in the treatment of cystitis glandularis.Methods: Twenty-four patients with cystitis glandularis were removed of the inducements,and then treated with intravesical instillation of HCPT after transurethral resection.Results: All the 24 cases were followed up.The recurrence was observed in 2 cases.The clinical symptoms of 18 cases disappeared and 4 were alleviated.Conclusions: Combination of the transurethral electrovaporization with HCPT intravesical instillation is an effective method in treating local recurrence of cystitis glandularis.
2006, 31(1): 27-29.
Abstract:
Objective: To improve the techniques of harvesting the free inferior epigastric artery transverse rectus abdominis myocutaneous(TRAM) flap on the basis of the anatomic studies.Methods: We performed breast reconstruction for four patients after mastectomy with this deep inferior epigastric perforator(DIEP) flap,sparing the whole rectus abdominis muscle,and preserving adequate abdominal wall competence.We also reviewed related literature and discussed its advantages.Results: This flap had all the advantages inherent in TRAM flap breast reconstruction and the donor site morbidities were avoided.The variety of the dimension and thickness of the flap and the length of its pedicle increased its flexibility in clinical application.Conclusions: DIEP flap can repair various defects as required.As it spares the whole rectus abdominis muscle,it has minimal insult to the abdominal wall and has the effect of abdominoplasty for middle-aged women.The application of this kind of flap represent the direction of plastic surgery's development.
Objective: To improve the techniques of harvesting the free inferior epigastric artery transverse rectus abdominis myocutaneous(TRAM) flap on the basis of the anatomic studies.Methods: We performed breast reconstruction for four patients after mastectomy with this deep inferior epigastric perforator(DIEP) flap,sparing the whole rectus abdominis muscle,and preserving adequate abdominal wall competence.We also reviewed related literature and discussed its advantages.Results: This flap had all the advantages inherent in TRAM flap breast reconstruction and the donor site morbidities were avoided.The variety of the dimension and thickness of the flap and the length of its pedicle increased its flexibility in clinical application.Conclusions: DIEP flap can repair various defects as required.As it spares the whole rectus abdominis muscle,it has minimal insult to the abdominal wall and has the effect of abdominoplasty for middle-aged women.The application of this kind of flap represent the direction of plastic surgery's development.
2006, 31(1): 30-31.
Abstract:
Objective: To explore the relationship between level of serum soluble Fas(sFas) and acute coronary syndrome(ACS).Methods: The level of sFas was measured in the sera from 22 patients with ACS and 10 controls by enzyme-linked immunosorbent assay(ELISA).The cardiac tropnin I(cTnI) of 21 ACS patients were measured at the same time.Results: The mean level of serum sFas was significantly higher in the patients with ACS than that of the controls(P<0.01).No difference was found between the groups with increased(MI) cTnI and normal(UA) cTnI in patients with ACS(P>0.05).Conclusions: High level of serum sFas is related to ACS,which may play an important role in the rupture of vulnerable atheromatous plaque.
Objective: To explore the relationship between level of serum soluble Fas(sFas) and acute coronary syndrome(ACS).Methods: The level of sFas was measured in the sera from 22 patients with ACS and 10 controls by enzyme-linked immunosorbent assay(ELISA).The cardiac tropnin I(cTnI) of 21 ACS patients were measured at the same time.Results: The mean level of serum sFas was significantly higher in the patients with ACS than that of the controls(P<0.01).No difference was found between the groups with increased(MI) cTnI and normal(UA) cTnI in patients with ACS(P>0.05).Conclusions: High level of serum sFas is related to ACS,which may play an important role in the rupture of vulnerable atheromatous plaque.
2006, 31(1): 32-33.
Abstract:
Objective: To study pathomorphologic characteristics of spleen Gaucher disease to improve the understanding,diagnosis and treatment of it,and to provide more information for clinical diagnosis.Methods: H-E,PAS staining and immunohistochemistry technique were used for the tissue sample.And literature concerned were reviewed.Results: The patient presented with enlarged liver and spleen.Gaucher cells had abundant cytoplasm containing distinct striations and were strong positive for PAS.Conclusions: Gaucher disease should be considered for the patient with chronic megalosplenia.Differential diagnosis should be made among diseases with splenomegalia.
Objective: To study pathomorphologic characteristics of spleen Gaucher disease to improve the understanding,diagnosis and treatment of it,and to provide more information for clinical diagnosis.Methods: H-E,PAS staining and immunohistochemistry technique were used for the tissue sample.And literature concerned were reviewed.Results: The patient presented with enlarged liver and spleen.Gaucher cells had abundant cytoplasm containing distinct striations and were strong positive for PAS.Conclusions: Gaucher disease should be considered for the patient with chronic megalosplenia.Differential diagnosis should be made among diseases with splenomegalia.
2006, 31(1): 34-37.
Abstract:
Objective: To explore the diagnostic significance of detection of myocardial troponin I mRNA(cTnI-mRNA) in peripheral blood for dilated cardiomyopathy.Methods: Reverse transcriptase polymerase chain reaction(RT-PCR) method was employed to detect cTnI-mRNA from the peripheral blood and nucleated cells of 32 patients with dilated cardiomyopathy.The serum levels of creatine kinase,isoenzyme,aspartate aminotranferase,lactate dehydrogenase and cTnI were studied at the same time.One blood sample from a patient receiving heart transplant was examined pathologically and twenty other blood samples from blood station were collected as control.Results: Swell cardiac myocytes,mitochondrial rupture,severe vacancylike denatureation,nucleus abnormality and condensed chromatin borders were observed by electron microscopy.The RT-PCR was all negative from nucleated cells,while fourteen positive RT-PCR were detected from peripheral blood.In the control group,no positive results were found from either the nucleated cell or peripheral blood.Of the 32 patients,positive enzymology was detected in six cases,positive cTnI was detected in five and fourteen had positive RT-PCR.The positive rate of RT-PCR was significantly higher than that of enzymology and cTnI detection(P<0.05 and P<0.01).Conclusions: cTnI-mRNA is located in the serum after myocardial injury occurred.RT-PCR method is more sensitive than enzynology and cTnI detection in the diagnosis of dilated cardiomyopathy.
Objective: To explore the diagnostic significance of detection of myocardial troponin I mRNA(cTnI-mRNA) in peripheral blood for dilated cardiomyopathy.Methods: Reverse transcriptase polymerase chain reaction(RT-PCR) method was employed to detect cTnI-mRNA from the peripheral blood and nucleated cells of 32 patients with dilated cardiomyopathy.The serum levels of creatine kinase,isoenzyme,aspartate aminotranferase,lactate dehydrogenase and cTnI were studied at the same time.One blood sample from a patient receiving heart transplant was examined pathologically and twenty other blood samples from blood station were collected as control.Results: Swell cardiac myocytes,mitochondrial rupture,severe vacancylike denatureation,nucleus abnormality and condensed chromatin borders were observed by electron microscopy.The RT-PCR was all negative from nucleated cells,while fourteen positive RT-PCR were detected from peripheral blood.In the control group,no positive results were found from either the nucleated cell or peripheral blood.Of the 32 patients,positive enzymology was detected in six cases,positive cTnI was detected in five and fourteen had positive RT-PCR.The positive rate of RT-PCR was significantly higher than that of enzymology and cTnI detection(P<0.05 and P<0.01).Conclusions: cTnI-mRNA is located in the serum after myocardial injury occurred.RT-PCR method is more sensitive than enzynology and cTnI detection in the diagnosis of dilated cardiomyopathy.
2006, 31(1): 38-39.
Abstract:
Objective: To explore the influence of carvedilol on the QT dispersion(QTd)in patients with acute myocardial infarction(AMI) at different times and its relation to the incidence of cardiac events.Methods: Fourty-three AMI patients were randomly divided into treatment group(23 cases) and control group(20 cases).The electrocardiogram(ECG)and Holter of the two groups were recorded and compared before therapy and at day 1,3,7,14 and 21 after therapy.The relationship between QTD and cardiac event was analyzed.Results: The QTd increased significantly at 24 h in patients with AMI but started to decrease at day 3,and the velocity was low.After 3 days of treatment with carediol,the QTd in the carwediol group decreased obviously compared with that in the control group(P<0.01).The incidence of cardiac events was 20% in the carvediol group while it was 34.8% in the control group(P>0.05).The max QTd of patients(77.19±10.81) with cardiac events was higher than that without cardiac events(58.15±9.71)(P<0.01).Conclusions: QTd increases obviously at the early stage of AMI.Carvediol may significantly lower the QTd of AMI and decrease the incidence of cardiac events.
Objective: To explore the influence of carvedilol on the QT dispersion(QTd)in patients with acute myocardial infarction(AMI) at different times and its relation to the incidence of cardiac events.Methods: Fourty-three AMI patients were randomly divided into treatment group(23 cases) and control group(20 cases).The electrocardiogram(ECG)and Holter of the two groups were recorded and compared before therapy and at day 1,3,7,14 and 21 after therapy.The relationship between QTD and cardiac event was analyzed.Results: The QTd increased significantly at 24 h in patients with AMI but started to decrease at day 3,and the velocity was low.After 3 days of treatment with carediol,the QTd in the carwediol group decreased obviously compared with that in the control group(P<0.01).The incidence of cardiac events was 20% in the carvediol group while it was 34.8% in the control group(P>0.05).The max QTd of patients(77.19±10.81) with cardiac events was higher than that without cardiac events(58.15±9.71)(P<0.01).Conclusions: QTd increases obviously at the early stage of AMI.Carvediol may significantly lower the QTd of AMI and decrease the incidence of cardiac events.
2006, 31(1): 40-42.
Abstract:
Objective: To study the value of umbilical artery flow velocity and nonstress test(NST) for patients with intrahepatic cholestasis in pregnancy(ICP).Methods: Fetal umbilical artery Doppler velocity and NST were used in 71 patients with ICP and 60 healthy women.Results: The incidence of S/D and abnormal NST was higher in the ICP group than that in the control group(P<0.05).The incidence of poor perinatal outcome in patients with abnormal S/D and NST was significantly higher than that of the control(P<0.005).Conclusions: Patients with ICP should receive examination of fetal umbilical artery blood flow and NST regularly in order to improve the perinatal prognosis.
Objective: To study the value of umbilical artery flow velocity and nonstress test(NST) for patients with intrahepatic cholestasis in pregnancy(ICP).Methods: Fetal umbilical artery Doppler velocity and NST were used in 71 patients with ICP and 60 healthy women.Results: The incidence of S/D and abnormal NST was higher in the ICP group than that in the control group(P<0.05).The incidence of poor perinatal outcome in patients with abnormal S/D and NST was significantly higher than that of the control(P<0.005).Conclusions: Patients with ICP should receive examination of fetal umbilical artery blood flow and NST regularly in order to improve the perinatal prognosis.
2006, 31(1): 42-43.
Abstract:
Objective: To evaluate the theraputic effect of microware irradiation combined with sodium aescinate(SA) injection for treating sudden deafness.Methods: The treatment group received microwave irradiation on the inner ear combined with SA injection and the control group were given SA injection only.Results: The total respond rate was 82.5% in the combination treatment group and 50.0% in the control group.The difference was significant(P<0.01).There was no obvious relation between the course of diseases and curative effect in the combination treatment group(P>0.05).Conclusions: It is effective to treat sudden deafness by microwave irradiation combined with SA injection.
Objective: To evaluate the theraputic effect of microware irradiation combined with sodium aescinate(SA) injection for treating sudden deafness.Methods: The treatment group received microwave irradiation on the inner ear combined with SA injection and the control group were given SA injection only.Results: The total respond rate was 82.5% in the combination treatment group and 50.0% in the control group.The difference was significant(P<0.01).There was no obvious relation between the course of diseases and curative effect in the combination treatment group(P>0.05).Conclusions: It is effective to treat sudden deafness by microwave irradiation combined with SA injection.
2006, 31(1): 44-45.
Abstract:
Objective: To investigate the prevention,diagnosis and treatment of the multiple organ failure in pregnant women with systemic lupus erythematosus(SLE).Methods: The clinical data of 7 cases of multiple organ failure induced by pregnancy SLE were analyzed retrospectively.Results: Two cases were diagnosed as SLE before pregnancy and 5 cases were diagnosed during hospital stay.Among them,6 cases were rescued and 1 case gave up.Six neonates died.Conclusions: The key to the treatment of pregnancy-induced SLE complicated by multiple organ failure are prompt diagnosis,effective treatment of the primary disease,timely termination of pregnancy,protection of the organ's function and intensifying the supportive therapy.
Objective: To investigate the prevention,diagnosis and treatment of the multiple organ failure in pregnant women with systemic lupus erythematosus(SLE).Methods: The clinical data of 7 cases of multiple organ failure induced by pregnancy SLE were analyzed retrospectively.Results: Two cases were diagnosed as SLE before pregnancy and 5 cases were diagnosed during hospital stay.Among them,6 cases were rescued and 1 case gave up.Six neonates died.Conclusions: The key to the treatment of pregnancy-induced SLE complicated by multiple organ failure are prompt diagnosis,effective treatment of the primary disease,timely termination of pregnancy,protection of the organ's function and intensifying the supportive therapy.
2006, 31(1): 46-48.
Abstract:
Objective: To evaluate ureteroscope pneumatic lithotripsy in the treatment of ureteral stones.Methods: Eighty-two cases of ureteral stones were treated with ureteroscope pneumatic lithotripsy.Results: Seventy-eight cases were successfully cured one time by this method,3 cases failed owing to the stenosis of the ureter and 1 case was halted due to the tortuosity of ureter complicated with polypus in the stones area.These 4 cases resorted to surgical operation.Ureteral small perforation occurred in 1 case and was managed with Double-J which was taken out after 1.5 months.Three months later no stenosis ureter was observed by IVP.Conclusions: Treatment of ureteral stones with ureteroscope pneumatic lithotriptor is a safe and effective method with few complications and a high successful rate.
Objective: To evaluate ureteroscope pneumatic lithotripsy in the treatment of ureteral stones.Methods: Eighty-two cases of ureteral stones were treated with ureteroscope pneumatic lithotripsy.Results: Seventy-eight cases were successfully cured one time by this method,3 cases failed owing to the stenosis of the ureter and 1 case was halted due to the tortuosity of ureter complicated with polypus in the stones area.These 4 cases resorted to surgical operation.Ureteral small perforation occurred in 1 case and was managed with Double-J which was taken out after 1.5 months.Three months later no stenosis ureter was observed by IVP.Conclusions: Treatment of ureteral stones with ureteroscope pneumatic lithotriptor is a safe and effective method with few complications and a high successful rate.
2006, 31(1): 48-50.
Abstract:
Objective: To study the efficacy and safety of 1-week triple therapy of omeprazole ilevofloxacin and furazolidone combined for Helicobacter pylori(Hp) eradication.Methods: One hundred patients with Hp infection suffering from chronic gastritis and peptic ulcer were randomized to treatment group and control group.The treatment group were administered omeprazole(20 mg,twice a day),levofloxacin(200 mg,twice a day) and furazolidone(100 mg,twice a day) for 1-week,while control group received omeprazole(20 mg,twice a day),clarithromycin(250 mg,twice a day) and metronidazole(400 mg,twice a day) for 1-week.The patients with peptic ulcer were treated with omeprazole(20 mg,every day) for another three weeks.Four weeks later,the Hp eradication,symptoms,peptic ulcer healing and side-effects were observed.Results: The Hp eradication rate,symptom ameliorate rate,peptic ulcer healing rate and side-effect rate between the treatment group and control group were 89.1% and 80.0%,93.5% and 88.9%,(92.5%) and 93.5%,21.74% and 24.44%,respectively.The difference was not significant(P>0.05).The cost of anti-Hp therapy of each patient was 107.82 Yuan(RMB) and 103.07 Yuan(RMB) in the treatment group and control group respectively.Conclusions: 1-week triple therapy of meprazole,levofloxacin and furazolidone combined is the first choice for Hp eradication.
Objective: To study the efficacy and safety of 1-week triple therapy of omeprazole ilevofloxacin and furazolidone combined for Helicobacter pylori(Hp) eradication.Methods: One hundred patients with Hp infection suffering from chronic gastritis and peptic ulcer were randomized to treatment group and control group.The treatment group were administered omeprazole(20 mg,twice a day),levofloxacin(200 mg,twice a day) and furazolidone(100 mg,twice a day) for 1-week,while control group received omeprazole(20 mg,twice a day),clarithromycin(250 mg,twice a day) and metronidazole(400 mg,twice a day) for 1-week.The patients with peptic ulcer were treated with omeprazole(20 mg,every day) for another three weeks.Four weeks later,the Hp eradication,symptoms,peptic ulcer healing and side-effects were observed.Results: The Hp eradication rate,symptom ameliorate rate,peptic ulcer healing rate and side-effect rate between the treatment group and control group were 89.1% and 80.0%,93.5% and 88.9%,(92.5%) and 93.5%,21.74% and 24.44%,respectively.The difference was not significant(P>0.05).The cost of anti-Hp therapy of each patient was 107.82 Yuan(RMB) and 103.07 Yuan(RMB) in the treatment group and control group respectively.Conclusions: 1-week triple therapy of meprazole,levofloxacin and furazolidone combined is the first choice for Hp eradication.
2006, 31(1): 51-52.
Abstract:
Objective: To evaluate the clinical value of domestic tubular stapler in transabdominal total gastrectomy.Methods: Fifty-eight cases of gastric cancer were treated by transabdominal total gastrectomy.Roux-en-Y modal of digestive reconstruction were accomplished after total gastrectomy for gastric cancer with domestic tubular stapler.Results: Fifty-seven cases were anastomosed by one stage,but 1 case had to be reanastomosed through the chest.No anastomotic leakage or hemorrhage was observed.During the follow-up of six monthes to two years,anastomotic stenosis occurred in one case.Conclusions: Familiarity with the function of domestic tubular stapler and proper operation can significantly shorten the surgery time,reduce blood loss,and alleviate tissue damage,reaching a satisfactory outcome.
Objective: To evaluate the clinical value of domestic tubular stapler in transabdominal total gastrectomy.Methods: Fifty-eight cases of gastric cancer were treated by transabdominal total gastrectomy.Roux-en-Y modal of digestive reconstruction were accomplished after total gastrectomy for gastric cancer with domestic tubular stapler.Results: Fifty-seven cases were anastomosed by one stage,but 1 case had to be reanastomosed through the chest.No anastomotic leakage or hemorrhage was observed.During the follow-up of six monthes to two years,anastomotic stenosis occurred in one case.Conclusions: Familiarity with the function of domestic tubular stapler and proper operation can significantly shorten the surgery time,reduce blood loss,and alleviate tissue damage,reaching a satisfactory outcome.
2006, 31(1): 53-54.
Abstract:
Objective: To evaluate the value of coronary anteriography in diagnosis of female angina.Methods: One hundred and eighteen female patients with angina had received coronary angiography.The resultant positive index of cornary angiography were retrospectively analyzed by reviewing male,electrocar digram,clinical menifestation and male case were compared.Results: In 118 cases of female,the incidence of normal coronary arteriography in angina patients were 43.20%.The female patient usually occurred single branch lesion.The incidence of abnormal coronary anteriography who have the changes of antierier wall was 75.68%.The incidence of abnormal coronary anteriography who had typical angina occurred in 80.00%.Conclusions: The changes in female angina patient was minor,but the rate of mistaken analysis was high.It is better to take coronary anteriography to make proper diagnosis and to lighten the economic and physical burden.
Objective: To evaluate the value of coronary anteriography in diagnosis of female angina.Methods: One hundred and eighteen female patients with angina had received coronary angiography.The resultant positive index of cornary angiography were retrospectively analyzed by reviewing male,electrocar digram,clinical menifestation and male case were compared.Results: In 118 cases of female,the incidence of normal coronary arteriography in angina patients were 43.20%.The female patient usually occurred single branch lesion.The incidence of abnormal coronary anteriography who have the changes of antierier wall was 75.68%.The incidence of abnormal coronary anteriography who had typical angina occurred in 80.00%.Conclusions: The changes in female angina patient was minor,but the rate of mistaken analysis was high.It is better to take coronary anteriography to make proper diagnosis and to lighten the economic and physical burden.
2006, 31(1): 55-56.
Abstract:
Objective: To investigate the incidence of metabolic syndrome and the related diseases in the elderly.Methods: The plasma glucose,lipid-profile,blood pressure and body mass index were measured for 532 aged men and women which receiving physical examination in our hospital.Results: The incidences of diabetes,hypertension,dislipidemia,overweight,obesity and metabolic syndrome were 18.98%,45.68%,50.38%,28.76%,7.52% and 13.35%,respectively.Conclusions: There is a high proportion of metabolic syndrome and related diseases in the elderly.
Objective: To investigate the incidence of metabolic syndrome and the related diseases in the elderly.Methods: The plasma glucose,lipid-profile,blood pressure and body mass index were measured for 532 aged men and women which receiving physical examination in our hospital.Results: The incidences of diabetes,hypertension,dislipidemia,overweight,obesity and metabolic syndrome were 18.98%,45.68%,50.38%,28.76%,7.52% and 13.35%,respectively.Conclusions: There is a high proportion of metabolic syndrome and related diseases in the elderly.
2006, 31(1): 72-74.
Abstract:
Objective: To explore the imaging features of primary central nervous system lymphomas(PCNSL) by CT and MRI.Methods: The imaging data of CT and MRI of 19 patients with pathologically proved PCNSL were retrospectively analyzed.Results: Fourteen cases had single lesion and 5 had multiple lesions.A total of 31 lesions were detected.Twelve lesions were on the surface of the brain,13 were about the ependyma and 6 in the depth of the brain.Twenty-six lesions were detected by CT,with a detection rate of 83.9%.Thirty-one lesions were detected by MRI,with a detection rate of 100%.T1WI and T2WI showed that the intensity of the tumor was similar to the gray matter and 22 lesions were homogeneous and 9 were inhomogeneous.Enhancement showed that 80.6% lesions were remarkably matter-enhancing,16.1% lesions had enpendymal or meningeal enhancement.The perifocal white matter edema and the mass effect were mild to moderate in most cases.Conclusions: CT combined with MRI findings can help to make preoperative diagnosis in most cases;however diagnosis of rare atypical cases is difficult.
Objective: To explore the imaging features of primary central nervous system lymphomas(PCNSL) by CT and MRI.Methods: The imaging data of CT and MRI of 19 patients with pathologically proved PCNSL were retrospectively analyzed.Results: Fourteen cases had single lesion and 5 had multiple lesions.A total of 31 lesions were detected.Twelve lesions were on the surface of the brain,13 were about the ependyma and 6 in the depth of the brain.Twenty-six lesions were detected by CT,with a detection rate of 83.9%.Thirty-one lesions were detected by MRI,with a detection rate of 100%.T1WI and T2WI showed that the intensity of the tumor was similar to the gray matter and 22 lesions were homogeneous and 9 were inhomogeneous.Enhancement showed that 80.6% lesions were remarkably matter-enhancing,16.1% lesions had enpendymal or meningeal enhancement.The perifocal white matter edema and the mass effect were mild to moderate in most cases.Conclusions: CT combined with MRI findings can help to make preoperative diagnosis in most cases;however diagnosis of rare atypical cases is difficult.
2006, 31(1): 75-77.
Abstract:
Objective: To summarize the CT features of adrenal tumors and to assess the value of spiral CT in diagnosis and differential diagnosis of the disease.Methods: The CT findings of 28 cases with pathologically proved adrenal tumors were analyzed retrospectively and the key points in diagnosis were presented.Results: Six cases had adenoma of middle size and density;4 had adenocarcinoma with irregular mass of uneven density and central low-density necrosis;9 cases had pheochromocytoma with a larger mass of heterogeneous density with marked enhancement;3 cases of myelolipoma were characterized by low density areas for fat contents;5 cases of metastasis,almost all of which resulted from the pulmonary cancer,displayed a round or irregular mass of heterogeneous density with middle-level enhancement;1 case of adrenal cyst presented low and heterogeneous density with thin and regular cyst wall.Conclusions: Adrenal tumors have certain CT characteristic signs.CT findings combined with clinical and laboratory data can provide correct diagnosis.
Objective: To summarize the CT features of adrenal tumors and to assess the value of spiral CT in diagnosis and differential diagnosis of the disease.Methods: The CT findings of 28 cases with pathologically proved adrenal tumors were analyzed retrospectively and the key points in diagnosis were presented.Results: Six cases had adenoma of middle size and density;4 had adenocarcinoma with irregular mass of uneven density and central low-density necrosis;9 cases had pheochromocytoma with a larger mass of heterogeneous density with marked enhancement;3 cases of myelolipoma were characterized by low density areas for fat contents;5 cases of metastasis,almost all of which resulted from the pulmonary cancer,displayed a round or irregular mass of heterogeneous density with middle-level enhancement;1 case of adrenal cyst presented low and heterogeneous density with thin and regular cyst wall.Conclusions: Adrenal tumors have certain CT characteristic signs.CT findings combined with clinical and laboratory data can provide correct diagnosis.
The diagnostic value of spiral CT in non-metal foreign bodies within trachea or bronchus of children
2006, 31(1): 77-79.
Abstract:
Objective: To evaluate spiral CT in the diagnosis of non-metal foreign bodies in trachea or bronchus in children.Methods: Twenty-three children suspected foreign bodies in trachea or bronchus were all checked by spiral CT combinated with multiplannar reconstruction(MPR),shaded surface display(SSD),and virtual bronchoscopy(CTVB),compared with the clinical data and fiberoptic bronchoscopic findings.Results: Spiral CT displayed directely all tracheobronchial foreign bodies,and showed endobronchial stenosis and occlusion,of these pulmonary emphysema in 11 children,atelectasis in 1 child,mediastinal double-side image in 10 children,pheumonia in 4 children,no foreign body in 8 child.The detective rate and accurate rate of spiral CT was 100% and 95.65% respectively.Conclusions: Spiral CT could direct display tracheobronchial Non-metal foreign bodies,and play an important role in fetching foreign bodies with fiberoptic bronchoscopy.
Objective: To evaluate spiral CT in the diagnosis of non-metal foreign bodies in trachea or bronchus in children.Methods: Twenty-three children suspected foreign bodies in trachea or bronchus were all checked by spiral CT combinated with multiplannar reconstruction(MPR),shaded surface display(SSD),and virtual bronchoscopy(CTVB),compared with the clinical data and fiberoptic bronchoscopic findings.Results: Spiral CT displayed directely all tracheobronchial foreign bodies,and showed endobronchial stenosis and occlusion,of these pulmonary emphysema in 11 children,atelectasis in 1 child,mediastinal double-side image in 10 children,pheumonia in 4 children,no foreign body in 8 child.The detective rate and accurate rate of spiral CT was 100% and 95.65% respectively.Conclusions: Spiral CT could direct display tracheobronchial Non-metal foreign bodies,and play an important role in fetching foreign bodies with fiberoptic bronchoscopy.
2006, 31(1): 80-82.
Abstract:
Objective: To study the changes and diagnostic value of coagulation function and relative variables of pretherombosis state in patients with pregnancy-induced hyertension(PIH).Methods: The prothrombin time(PT),activated partial prothrombin time(APTT),fibrinogen(Fbg),vWF,GMP-140,TAT,and were examined in 30 normal late-pregnancy women,30 non-pregnancy women and 40 patients with PIH.Results: In the normal late-pregnancy women and patients with PIH,the levels of PT was obviously lower(P<0.01),while the level of APTT had no significant change(P>0.05) compared with the non-pregnant women;the level of vWF,GMP-140 and TAT were obviously higher in PIH women(P<0.01)than in the non-pregnant and late-pregnant women;some variables,including Fbg and D-dimer were greatly higher in patients with PIH than in the non-pregnant women(P<0.01);further more,these changes became more apparent with the progression of the disease.Conclusions: The normal late-pregnant women and the PIH women are in the hypercoagulation and prethrombotic state.Antenatal examination of coagulation function and variables of prethrombosis state are important for the prevention and treatment of PIH.
Objective: To study the changes and diagnostic value of coagulation function and relative variables of pretherombosis state in patients with pregnancy-induced hyertension(PIH).Methods: The prothrombin time(PT),activated partial prothrombin time(APTT),fibrinogen(Fbg),vWF,GMP-140,TAT,and were examined in 30 normal late-pregnancy women,30 non-pregnancy women and 40 patients with PIH.Results: In the normal late-pregnancy women and patients with PIH,the levels of PT was obviously lower(P<0.01),while the level of APTT had no significant change(P>0.05) compared with the non-pregnant women;the level of vWF,GMP-140 and TAT were obviously higher in PIH women(P<0.01)than in the non-pregnant and late-pregnant women;some variables,including Fbg and D-dimer were greatly higher in patients with PIH than in the non-pregnant women(P<0.01);further more,these changes became more apparent with the progression of the disease.Conclusions: The normal late-pregnant women and the PIH women are in the hypercoagulation and prethrombotic state.Antenatal examination of coagulation function and variables of prethrombosis state are important for the prevention and treatment of PIH.
2006, 31(1): 83-84.
Abstract:
Objective: To compare the test results by the dry and the liquid biochemical analyzers.Methods: Forty-four blood samples without hemolysis and hyperlipidemia were analyzed of the alanine aminotransferase(ALT),aspartate aminotransferase(AST),total protein(TP),albumin(ALB),total bilirubin(TB),uric acid(UA),blood urea nitrogen(BUN) and serum creatinine(Scr) in SPOTCHEMTMⅡ SP-4430 and in OLYMPUS AU2700 chemical analyzer.Results: The test results of BUN,TB,TP were statistically different by the two methods(P<0.05 to P<0.001),but the difference was not significant in those of ALT,AST,ALB,UA and Scr(P>0.05).Conclusions: There are great differences in some of the test results by the dry and the liquid chemical methods.And it is essential to analyze the results by the two methods and calibrate the chemical analyzers so as to assure their accuracy.
Objective: To compare the test results by the dry and the liquid biochemical analyzers.Methods: Forty-four blood samples without hemolysis and hyperlipidemia were analyzed of the alanine aminotransferase(ALT),aspartate aminotransferase(AST),total protein(TP),albumin(ALB),total bilirubin(TB),uric acid(UA),blood urea nitrogen(BUN) and serum creatinine(Scr) in SPOTCHEMTMⅡ SP-4430 and in OLYMPUS AU2700 chemical analyzer.Results: The test results of BUN,TB,TP were statistically different by the two methods(P<0.05 to P<0.001),but the difference was not significant in those of ALT,AST,ALB,UA and Scr(P>0.05).Conclusions: There are great differences in some of the test results by the dry and the liquid chemical methods.And it is essential to analyze the results by the two methods and calibrate the chemical analyzers so as to assure their accuracy.
2006, 31(1): 85-86.
Abstract:
Objective: To explore the features and significance of myelogram of patients with simultaneous reduction of the ternary systems i.e.peripheral blood hemoglobin(Hb),white blood cell(WBC) and blood platelet count(BPC).Methods: The bone marrow punctures were made on 211 cases patients suffered from the peripheral blood ternary sorts cells simultaneous reductions to carry out the cytomorphology tests.Results: Among the 196 cases(other 15 cases' marrow without special changes) which were identified with myelogram,55 cases(26.4%) were diagnosed as malignant blood system diseases,107 cases(50.7%),as diverse anemia,18 cases(8.5%),as hypersplenism and 16 cases(7.6%),as others.Conclusions: Most cases of peripheral blood ternary sorts cells simultaneous reductions can be found in bone marrow and the myelogram changes of patients manifest diversifications.A good master of these features of changes will facilitate to assay the causes and improve the myelogram diagnostic level.
Objective: To explore the features and significance of myelogram of patients with simultaneous reduction of the ternary systems i.e.peripheral blood hemoglobin(Hb),white blood cell(WBC) and blood platelet count(BPC).Methods: The bone marrow punctures were made on 211 cases patients suffered from the peripheral blood ternary sorts cells simultaneous reductions to carry out the cytomorphology tests.Results: Among the 196 cases(other 15 cases' marrow without special changes) which were identified with myelogram,55 cases(26.4%) were diagnosed as malignant blood system diseases,107 cases(50.7%),as diverse anemia,18 cases(8.5%),as hypersplenism and 16 cases(7.6%),as others.Conclusions: Most cases of peripheral blood ternary sorts cells simultaneous reductions can be found in bone marrow and the myelogram changes of patients manifest diversifications.A good master of these features of changes will facilitate to assay the causes and improve the myelogram diagnostic level.
2006, 31(1): 90-92.
Abstract:
Objective: To study the application of intravenous catheter system in the oncology department,and to explore an effective nursing method.Methods: The 4th generation intravenous catheter sealed with single-wing,made in America,was applied to 68 patients receiving infusion therapy.The large-angle,slow and direct inserting method was applied to Group A,and the scalp needle inserting method to Group B.The relationship between the inserting methods and retaining time was studied.The veins in the neck,cubital fossa and two sides of forearm were selected in main-vein group and peripheral veins of the limbs were selected in branch-vein group.The relationship between the inserting position,the medicine and phlebitis was studied.Results: Successful retaining for more than 48 hours accounted for 88.64% in Group A,and 33.33% in group B(P<0.005);When medicine containing chemotherapy was infused,phlebitis occurred in 53.85% of the cases in the main-vein group and 87.50% in the branch-vein group(P<0.01).When medicine containing no chemotherapy was infused,phlebitis developed in(14.29%) of the cases in the main-vein group and 58.33% in the branch-vein group(P<0.05).When the vein conditions were almost the same,phlebitis occurred in(66.67%) of the cases receiving medicine containing chemotherapy and 34.62% receiving medicine containing no chemotherapy(P<0.01).Conclusions: The large angle,slow and straight inserting method may effectively prolong the retaining time of intravenous catheter and decrease the occurrence of phlebitis.Medicine containing chemotherapy,which irritates the veins greatly,is more liable to cause phlebitis.The intravenous catheter system can partly take the place of scalp needle in the oncology department.
Objective: To study the application of intravenous catheter system in the oncology department,and to explore an effective nursing method.Methods: The 4th generation intravenous catheter sealed with single-wing,made in America,was applied to 68 patients receiving infusion therapy.The large-angle,slow and direct inserting method was applied to Group A,and the scalp needle inserting method to Group B.The relationship between the inserting methods and retaining time was studied.The veins in the neck,cubital fossa and two sides of forearm were selected in main-vein group and peripheral veins of the limbs were selected in branch-vein group.The relationship between the inserting position,the medicine and phlebitis was studied.Results: Successful retaining for more than 48 hours accounted for 88.64% in Group A,and 33.33% in group B(P<0.005);When medicine containing chemotherapy was infused,phlebitis occurred in 53.85% of the cases in the main-vein group and 87.50% in the branch-vein group(P<0.01).When medicine containing no chemotherapy was infused,phlebitis developed in(14.29%) of the cases in the main-vein group and 58.33% in the branch-vein group(P<0.05).When the vein conditions were almost the same,phlebitis occurred in(66.67%) of the cases receiving medicine containing chemotherapy and 34.62% receiving medicine containing no chemotherapy(P<0.01).Conclusions: The large angle,slow and straight inserting method may effectively prolong the retaining time of intravenous catheter and decrease the occurrence of phlebitis.Medicine containing chemotherapy,which irritates the veins greatly,is more liable to cause phlebitis.The intravenous catheter system can partly take the place of scalp needle in the oncology department.
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