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2006 Vol. 31, No. 2
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2006, 31(2): 116-118.
Abstract:
Objective: To explore the effect of StaphylococcuS.aureus(S.aureus) L-forms on apoptosis of murine thymocytes.Methods: Murine thymocytes were co-cultured with S.aureus,S.aureus L-forms or staphylococcal enterotoxin B(SEB) in vitro for different times(2 hrs,4 hrs,6 hrs,8 hrs,or 10 hrs) before examination.The apoptotic rates of murine thymocytes were measured by flowcytometry technique and using Annexin V-FITC/PI double staining kit.Results: The apoptotic rates of thymocytes in group of SEB treated for 2 hrs,in group of S.aureus infected for 4 hrs,and in group of S.aureus L-forms infected for 6 hrs were obviously increased compared to that of the control(all groups showed P<0.01).The longer the time of incubation,the higher rates of thymocyte apoptosis.Conclusions: Both S.aureus L-forms and S.aureus can induce the apoptosis of murine thymocytes.Except for SEB,other factors might be involved in the apoptosis of thymocytes induced by S.aureus and S.aureus L-forms that producing SEB.
Objective: To explore the effect of StaphylococcuS.aureus(S.aureus) L-forms on apoptosis of murine thymocytes.Methods: Murine thymocytes were co-cultured with S.aureus,S.aureus L-forms or staphylococcal enterotoxin B(SEB) in vitro for different times(2 hrs,4 hrs,6 hrs,8 hrs,or 10 hrs) before examination.The apoptotic rates of murine thymocytes were measured by flowcytometry technique and using Annexin V-FITC/PI double staining kit.Results: The apoptotic rates of thymocytes in group of SEB treated for 2 hrs,in group of S.aureus infected for 4 hrs,and in group of S.aureus L-forms infected for 6 hrs were obviously increased compared to that of the control(all groups showed P<0.01).The longer the time of incubation,the higher rates of thymocyte apoptosis.Conclusions: Both S.aureus L-forms and S.aureus can induce the apoptosis of murine thymocytes.Except for SEB,other factors might be involved in the apoptosis of thymocytes induced by S.aureus and S.aureus L-forms that producing SEB.
2006, 31(2): 119-121.
Abstract:
Objective: To construct glial cell line-derived neurotrophic growth factor（GDNF） gene fluorescent eukaryotic expression plasmid.Methods: The coding sequence of GDNF was amplified by PCR,the GDNF gene was cloned into plasmid pEGFP-N1,and the recombinant vector was selected and identified by restriction enzyme analysis and nucleotide sequence determination.Results: Correct construction of pEGFP-N1-GDNF was identified by methods of restriction enzyme analysis,and nucleotide sequence determination.Conclusions: An eukaryotic expression plasmid pEGFP-N1-GDNF has been constructed successfully,which will provide the basis for studying the gene of GDNF.
Objective: To construct glial cell line-derived neurotrophic growth factor（GDNF） gene fluorescent eukaryotic expression plasmid.Methods: The coding sequence of GDNF was amplified by PCR,the GDNF gene was cloned into plasmid pEGFP-N1,and the recombinant vector was selected and identified by restriction enzyme analysis and nucleotide sequence determination.Results: Correct construction of pEGFP-N1-GDNF was identified by methods of restriction enzyme analysis,and nucleotide sequence determination.Conclusions: An eukaryotic expression plasmid pEGFP-N1-GDNF has been constructed successfully,which will provide the basis for studying the gene of GDNF.
2006, 31(2): 121-123.
Abstract:
Objective: To investigate the inhibitory effects of Genistein on the proliferation of B16 melanoma cells.Methods: B16 melanoma cells were treated with 10,30,90 μmol/L Genistein for 1-4 days.Growth curve test was served as the index to reflect prolifertion activity of B16 melanoma cells,Morphological changes of B16 melanoma cells,melanin content in the supernatant of melanoma cells,distribution of cell cycle were measured by Flow Cytometery.Results: The Genistein at the oncentration of 10,30,90 μmol/L inhibited significantly the growth of B16 melanoma cells in dose dependent manner with statistical significance(P<0.05-P<0.01 vs control).Interestingly,Genistein induced the differentiation of B16 melanoma cells which showed that melanic granule and number of cells decreased.Flow cytometry showed that numerous cells were retarded at S phase of cell cycle.Conclusions: The Genistein at the dose of 10,30,90 μmol/L significantly inhibit the growth of B16cells.
Objective: To investigate the inhibitory effects of Genistein on the proliferation of B16 melanoma cells.Methods: B16 melanoma cells were treated with 10,30,90 μmol/L Genistein for 1-4 days.Growth curve test was served as the index to reflect prolifertion activity of B16 melanoma cells,Morphological changes of B16 melanoma cells,melanin content in the supernatant of melanoma cells,distribution of cell cycle were measured by Flow Cytometery.Results: The Genistein at the oncentration of 10,30,90 μmol/L inhibited significantly the growth of B16 melanoma cells in dose dependent manner with statistical significance(P<0.05-P<0.01 vs control).Interestingly,Genistein induced the differentiation of B16 melanoma cells which showed that melanic granule and number of cells decreased.Flow cytometry showed that numerous cells were retarded at S phase of cell cycle.Conclusions: The Genistein at the dose of 10,30,90 μmol/L significantly inhibit the growth of B16cells.
2006, 31(2): 124-126.
Abstract:
Objective: To explore the alterative mechanism of intestinal mucosal cell membrane fluidity and calcium pump in rats with acute pancreatitis(AP),and to study the effects of emodin and somatostatin on them.Methods: Eighty male SD rats were randomly divided into four groups:sham operation group(SO),normal saline(NS) group,emodin group and somatostatin group.Intestinal mucosal cell membrane Ca2+-Mg2+ATPase activity,fluorescence polarization,malondialdehyde(MDA) content and blood flow of intestinal mucosa were determined 6 hours after the model rats of acute pancreatitis were established.Results: In the emodin group and somatostatin group,the MDA content was lower than that in the SO group(P<0.01),Ca2+-Mg2+ATPase activity and membrane fluidity were higher than those in SO group(P<0.01)and the decreased value of blood flow of intestinal mucosa was lower compared with that of SO group.Conclusions: Emodin and somatostatin can protect the function of intestinal mucosal barrier by ameliorating intestinal mucosa ischemia,improving calcium pump activity and elevating intestinal mucosal cell membrane fluidity.
Objective: To explore the alterative mechanism of intestinal mucosal cell membrane fluidity and calcium pump in rats with acute pancreatitis(AP),and to study the effects of emodin and somatostatin on them.Methods: Eighty male SD rats were randomly divided into four groups:sham operation group(SO),normal saline(NS) group,emodin group and somatostatin group.Intestinal mucosal cell membrane Ca2+-Mg2+ATPase activity,fluorescence polarization,malondialdehyde(MDA) content and blood flow of intestinal mucosa were determined 6 hours after the model rats of acute pancreatitis were established.Results: In the emodin group and somatostatin group,the MDA content was lower than that in the SO group(P<0.01),Ca2+-Mg2+ATPase activity and membrane fluidity were higher than those in SO group(P<0.01)and the decreased value of blood flow of intestinal mucosa was lower compared with that of SO group.Conclusions: Emodin and somatostatin can protect the function of intestinal mucosal barrier by ameliorating intestinal mucosa ischemia,improving calcium pump activity and elevating intestinal mucosal cell membrane fluidity.
2006, 31(2): 126-128.
Abstract:
Objective: To investigate the effects of two mediums supplemented with various ingredient on epidermal melanocytes culture.Methods: The foreskins from an infant were treated with dispase II to separate epidermis from dermis,and treated with trypsin for obtaining single cell suspensions.The cells were cultured in different media supplemented with cell growth component.The proliferation and dendrites of cultured melanocytes were observed day by day.Results: The melanocytes from infant foreskin showed potent vitality.The fibroblasts cells in culture decreased after dispase and trypsin digesting specimen.The rapid proliferation and increased dendrites of melanocytes in 254 media supplemented with human melanocyte growth supplement(HMGS) were observed.Conclusions: Infant foreskins treated with dispase and trypsin and cultured with 254 containing HMGS,were suitable for melanocyte culture.
Objective: To investigate the effects of two mediums supplemented with various ingredient on epidermal melanocytes culture.Methods: The foreskins from an infant were treated with dispase II to separate epidermis from dermis,and treated with trypsin for obtaining single cell suspensions.The cells were cultured in different media supplemented with cell growth component.The proliferation and dendrites of cultured melanocytes were observed day by day.Results: The melanocytes from infant foreskin showed potent vitality.The fibroblasts cells in culture decreased after dispase and trypsin digesting specimen.The rapid proliferation and increased dendrites of melanocytes in 254 media supplemented with human melanocyte growth supplement(HMGS) were observed.Conclusions: Infant foreskins treated with dispase and trypsin and cultured with 254 containing HMGS,were suitable for melanocyte culture.
2006, 31(2): 129-131.
Abstract:
Objective: To investigate the role of amniotic membrane in the prevention of epidural adhesion after laminectomy and to find out the related mechanism.Methods: Laminectomy was performed in the lumbar 5 sector of 60 rabbits,and the exposed dura mater was covered with amniotic membrane(group AM),chitosan membrane(group CHS),and no covering in the control group(group CON) respectively.Five animals in each group were sacrificed at 2,4,8,12 weeks postoperatively.By using a double blind protocol,the specimens were prepared for histological study and computer-assisted image analysis.Results: In the control group,the adhesion of the dura mater to the scar tissue occurred to the entire exposed surface and the extradural space almost disappeared.In group AM and CHS,the formation of epidural scar was much less than that in the control group.The dura mater was smooth and a gap had formed in the epidural cavity.The epidural space was well preserved.Group AM had the lowest adhesion evaluation index,and there was statistical difference(P<0.05-P<0.01).Conclusions: Putting a suitable obstructer between the dura mater and the erector spinal muscle can prevent the extension of epidural scar into the spinal canal and make dura exempted from adhesion.Amniotic membrane is an effective biocompatible material in preventing epidural scar.
Objective: To investigate the role of amniotic membrane in the prevention of epidural adhesion after laminectomy and to find out the related mechanism.Methods: Laminectomy was performed in the lumbar 5 sector of 60 rabbits,and the exposed dura mater was covered with amniotic membrane(group AM),chitosan membrane(group CHS),and no covering in the control group(group CON) respectively.Five animals in each group were sacrificed at 2,4,8,12 weeks postoperatively.By using a double blind protocol,the specimens were prepared for histological study and computer-assisted image analysis.Results: In the control group,the adhesion of the dura mater to the scar tissue occurred to the entire exposed surface and the extradural space almost disappeared.In group AM and CHS,the formation of epidural scar was much less than that in the control group.The dura mater was smooth and a gap had formed in the epidural cavity.The epidural space was well preserved.Group AM had the lowest adhesion evaluation index,and there was statistical difference(P<0.05-P<0.01).Conclusions: Putting a suitable obstructer between the dura mater and the erector spinal muscle can prevent the extension of epidural scar into the spinal canal and make dura exempted from adhesion.Amniotic membrane is an effective biocompatible material in preventing epidural scar.
2006, 31(2): 132-133.
Abstract:
Objective: To study the prevailing characteristics of the infantile Mycoplasma pneumoniae infection,its relations with the age,season,sex and the initial symptoms after MP infection.Methods: The MP-Ab in the serum of some children under the age of 14 who were treated in our hospital from Jan.1999 to Dec.2004 was measure by the method of passive particle agglutination.Results: The positive rate of!MP-Ab had certain laws:there was significant difference between the four seasons(P<0.01);the difference was significant between the male and the female(P<0.005);It was commonly seen in the preschool children(32.24%) and the school children(40.63%).The symptoms outside the lung caused by MP infection tended to increase.Conclusions: The MP infection is closely related with the age,season and sex.The lesion outside the lung caused by MP infection has the tendency to increase in recent years.
Objective: To study the prevailing characteristics of the infantile Mycoplasma pneumoniae infection,its relations with the age,season,sex and the initial symptoms after MP infection.Methods: The MP-Ab in the serum of some children under the age of 14 who were treated in our hospital from Jan.1999 to Dec.2004 was measure by the method of passive particle agglutination.Results: The positive rate of!MP-Ab had certain laws:there was significant difference between the four seasons(P<0.01);the difference was significant between the male and the female(P<0.005);It was commonly seen in the preschool children(32.24%) and the school children(40.63%).The symptoms outside the lung caused by MP infection tended to increase.Conclusions: The MP infection is closely related with the age,season and sex.The lesion outside the lung caused by MP infection has the tendency to increase in recent years.
2006, 31(2): 134-136,171.
Abstract:
Objective: To approach the feature of MRI and clinic of brain cysticercosis.Methods: The retrospective analysis was adopted in clinical data and head MRI of 81 patients with brain cysticercosis.Results: The MRI appearance included three types,which was 73 brain parenchyma type,5 encephalocoele type and 3 mixed type,four stages which was vivi-worm phase,cataplasia and death phase,inactive phase and confounding phase,brain parenchyma type and cataplasia and death phase were saw frequently among them.Generally 3-5 courses of treatment were required to anthelmintic.Conclusions: The MRI can precisely type,stage and position brain cysticercosis,it is helpful for definition in clinical medicine and course of treatment,and it is used to diagnosis CT-negative brain cysticercosis.
Objective: To approach the feature of MRI and clinic of brain cysticercosis.Methods: The retrospective analysis was adopted in clinical data and head MRI of 81 patients with brain cysticercosis.Results: The MRI appearance included three types,which was 73 brain parenchyma type,5 encephalocoele type and 3 mixed type,four stages which was vivi-worm phase,cataplasia and death phase,inactive phase and confounding phase,brain parenchyma type and cataplasia and death phase were saw frequently among them.Generally 3-5 courses of treatment were required to anthelmintic.Conclusions: The MRI can precisely type,stage and position brain cysticercosis,it is helpful for definition in clinical medicine and course of treatment,and it is used to diagnosis CT-negative brain cysticercosis.
2006, 31(2): 136-137.
Abstract:
Objective: To explore the clinical effects of reconstruction of maxilla defects with buccal fat pad and radial forearm flap.Methods: Six cases of maxilla defects were reconstructed using buccal fat pad and radial forearm flap.The clinical effects were evaluated by the vitality of the flap,and the function of speech,swallowing and breath.Results: All the flaps were alive.No hindrance to speech,swallowing or breath was observed.Conclusions: It is a good method to reconstruct the defects of maxilla with buccal fat pad and radial forearm flap,which greatly improves the life quality of the patients.
Objective: To explore the clinical effects of reconstruction of maxilla defects with buccal fat pad and radial forearm flap.Methods: Six cases of maxilla defects were reconstructed using buccal fat pad and radial forearm flap.The clinical effects were evaluated by the vitality of the flap,and the function of speech,swallowing and breath.Results: All the flaps were alive.No hindrance to speech,swallowing or breath was observed.Conclusions: It is a good method to reconstruct the defects of maxilla with buccal fat pad and radial forearm flap,which greatly improves the life quality of the patients.
2006, 31(2): 138-140.
Abstract:
Objective: To analyze the key points in surgical management of sphenoid ridge meningioma.Methods: The clinical dada of 17 patients with sphenoid ridge meningioma were analyzed retrospectively.Results: Tumors were resected totally in 13 cases,subtotally in 3 cases and partially in 1 case.The visual disturbance occurred in 2 patients and hemiplegia in 1.Conclusions: The sphenoid ridge meningiomas should be resected as completely as possible on the premises of preserving the nerve and artery.The postoperative residual tumour should receive radiotherapy.
Objective: To analyze the key points in surgical management of sphenoid ridge meningioma.Methods: The clinical dada of 17 patients with sphenoid ridge meningioma were analyzed retrospectively.Results: Tumors were resected totally in 13 cases,subtotally in 3 cases and partially in 1 case.The visual disturbance occurred in 2 patients and hemiplegia in 1.Conclusions: The sphenoid ridge meningiomas should be resected as completely as possible on the premises of preserving the nerve and artery.The postoperative residual tumour should receive radiotherapy.
2006, 31(2): 140-141.
Abstract:
Objective: To observe the cardiovascular response during extubation in patients who have received general anesthesia by vein injection of tramadol.Methods: Forty patients were randomly divided into observation group and control group,with 20 patients in each.Use of anesthesia was halted half hour before the completion of operation,then tramadol 1.5 mg/kg was administered intravenously to the patients in the observation group,but no medicine was given to the control group.The SBP,DBP,HR at the moment of injection,the time of phlegm sucking,the moment of extubation and 1 minute and 5 minutes after extubation were observed.Results: The SBP,DBP and HR increased significantly at the moment of phlegm sucking,extubation,and 1 minute after extubation in the control group,compared with those at the moment of injection(P<0.01).And The SBP,DBP and HR decreased slightly at 5 minutes after extubation;however the SBP was still higher than that at the moment of injection(P<0.05).In the observation group,the SBP,DBP,HR were stable and increased slightly during the above mentioned moments,and their difference with the moment of injection was not significant(P>0.05).The patients come back stably,with no mania or pain on the wound.Conclusions: Tramadol can effectively relieve the cardiovascular response at extubation for patients receiving general anesthesia.
Objective: To observe the cardiovascular response during extubation in patients who have received general anesthesia by vein injection of tramadol.Methods: Forty patients were randomly divided into observation group and control group,with 20 patients in each.Use of anesthesia was halted half hour before the completion of operation,then tramadol 1.5 mg/kg was administered intravenously to the patients in the observation group,but no medicine was given to the control group.The SBP,DBP,HR at the moment of injection,the time of phlegm sucking,the moment of extubation and 1 minute and 5 minutes after extubation were observed.Results: The SBP,DBP and HR increased significantly at the moment of phlegm sucking,extubation,and 1 minute after extubation in the control group,compared with those at the moment of injection(P<0.01).And The SBP,DBP and HR decreased slightly at 5 minutes after extubation;however the SBP was still higher than that at the moment of injection(P<0.05).In the observation group,the SBP,DBP,HR were stable and increased slightly during the above mentioned moments,and their difference with the moment of injection was not significant(P>0.05).The patients come back stably,with no mania or pain on the wound.Conclusions: Tramadol can effectively relieve the cardiovascular response at extubation for patients receiving general anesthesia.
2006, 31(2): 142-144.
Abstract:
Objective: To explore the optimal management of idiopathic thrombocytopenic purpura(ITP) in pregnancy.Methods: The data of 18 cases of ITP in pregnancy were studied retrospectively.For the patients with ITP,prednisone or intravenous immunoglobulin(IVIG) was the first choice.Short-term platelet transfusion was given to those with platelet count less than(50×109/L).Results: Among the 18 cases,vaginal delivery and cesarean section were performed in 6 and 12 cases,respectively;the occurrence of postpartum hemorrhage was 11.1%.No maternal death occurred,and no neonatal thrombocytopenic was observed.Conclusions: We must reinforce obstetrical surveillance and management of ITP in pregnancy.We suggest that ITP mothers without obstetrical complications adopt vaginal delivery.Cesarean delivery should be performed to those whose platelet count is less than 50×109/L or with other obstetrical complications when there is sufficient fresh blood and platelet.
Objective: To explore the optimal management of idiopathic thrombocytopenic purpura(ITP) in pregnancy.Methods: The data of 18 cases of ITP in pregnancy were studied retrospectively.For the patients with ITP,prednisone or intravenous immunoglobulin(IVIG) was the first choice.Short-term platelet transfusion was given to those with platelet count less than(50×109/L).Results: Among the 18 cases,vaginal delivery and cesarean section were performed in 6 and 12 cases,respectively;the occurrence of postpartum hemorrhage was 11.1%.No maternal death occurred,and no neonatal thrombocytopenic was observed.Conclusions: We must reinforce obstetrical surveillance and management of ITP in pregnancy.We suggest that ITP mothers without obstetrical complications adopt vaginal delivery.Cesarean delivery should be performed to those whose platelet count is less than 50×109/L or with other obstetrical complications when there is sufficient fresh blood and platelet.
2006, 31(2): 144-145.
Abstract:
Objective: To introduce a new method of treating blepharoptosis.Methods: Thirty-six cases of blepharoptosis were corrected by direct suspension of the frontalis-aponeurosis.The frontalis-aponeurosis was isolated from the subcutis and a 1.5 cm incision was cut upper the margin of eyebrow.And through the incision,the frontalis-aponeurosis on the superficies of periosteam was dissected 1.5 cm to the upper margin of orbital and the superficiesaponeurosis was pulled down and fixed to the levator rnuscle-aponeurosis by mattress sutures,with the tension being adjusted to a moderate degree.Results: Thirty six cases of blepharoptosis were cured by primary hedling with this method.The patients were followed up for 6 to 12 months with satisfactory results and no recurrences.Conclusions: Compared with the traditional methods,this one may decrease the damage to the upper lid and frontalis area,leaving no risk of damaging the vessels or nerves.The technique is simple and the curative effect is affirmed.It is the first choice for treatment of blepharoptosis.
Objective: To introduce a new method of treating blepharoptosis.Methods: Thirty-six cases of blepharoptosis were corrected by direct suspension of the frontalis-aponeurosis.The frontalis-aponeurosis was isolated from the subcutis and a 1.5 cm incision was cut upper the margin of eyebrow.And through the incision,the frontalis-aponeurosis on the superficies of periosteam was dissected 1.5 cm to the upper margin of orbital and the superficiesaponeurosis was pulled down and fixed to the levator rnuscle-aponeurosis by mattress sutures,with the tension being adjusted to a moderate degree.Results: Thirty six cases of blepharoptosis were cured by primary hedling with this method.The patients were followed up for 6 to 12 months with satisfactory results and no recurrences.Conclusions: Compared with the traditional methods,this one may decrease the damage to the upper lid and frontalis area,leaving no risk of damaging the vessels or nerves.The technique is simple and the curative effect is affirmed.It is the first choice for treatment of blepharoptosis.
2006, 31(2): 146-148.
Abstract:
Objective: To evaluate the efficacy and toxicity reactions of domestic paclitaxel plus cisplatin in the treatment of advanced non-small cell lung cancer(NSCLC).Methods: Forty previously untreated patients with advanced NSCLC were selected and domestic paclitaxel was given at a dose of 135 mg/m2 on day 1 and cisplatin at a dose of 30 mg/m2 on day 1 to 3.The chemotherapy was repeated every 21 days with two cycles.Tumor supplied group of factors(TSGF) was measured by biochemical method.Results: The overall response rate was 42.9%.The main maintoxicity reactions were nausea/vomiting(11.4% were in gradeⅢ to Ⅳ) and hematologic toxicity(the prevalence of grade Ⅲ to Ⅳ leukopenia decreased to 14.2%).The serum level of TSGF decreased significantly(P<0.001)in the response patients after two cycles of chemotherapy but the change of serum level of TSGF was not obvious in the non-response patients.Conclusions: The combination therapy of paclitaxel plus cisplatin is a highly effective regimen for patients with advanced NSCLC with a manageable toxicity.
Objective: To evaluate the efficacy and toxicity reactions of domestic paclitaxel plus cisplatin in the treatment of advanced non-small cell lung cancer(NSCLC).Methods: Forty previously untreated patients with advanced NSCLC were selected and domestic paclitaxel was given at a dose of 135 mg/m2 on day 1 and cisplatin at a dose of 30 mg/m2 on day 1 to 3.The chemotherapy was repeated every 21 days with two cycles.Tumor supplied group of factors(TSGF) was measured by biochemical method.Results: The overall response rate was 42.9%.The main maintoxicity reactions were nausea/vomiting(11.4% were in gradeⅢ to Ⅳ) and hematologic toxicity(the prevalence of grade Ⅲ to Ⅳ leukopenia decreased to 14.2%).The serum level of TSGF decreased significantly(P<0.001)in the response patients after two cycles of chemotherapy but the change of serum level of TSGF was not obvious in the non-response patients.Conclusions: The combination therapy of paclitaxel plus cisplatin is a highly effective regimen for patients with advanced NSCLC with a manageable toxicity.
2006, 31(2): 148-149.
Abstract:
Objective: To explore the effect of cyclosporin A(CsA) in reversing multidrug resistance(MDR) in patients with acute leukemia(AL).Methods: Twenty-eight cases of acute leukemia were randomly divided into therapeutic group(CsA plus chemotherapy) and control group(chemotherapy alone).The expression of P-glycoprotein(P-gp) was assayed in 16 cases of the therapeutic group.Results: The total effective rate was 68.8% and the complete remission(CR) rate of the patients receiving repetition treatment was(7/10) in the therapeutic group,while the total effective rate was 25.0% and the CR rate of the patients receiving repetition treatment was 1/8 in the control group.The difference was significant(P<0.05).Conclusions: The CsA combined with chemotherapy may reverse MDR in patients with acute leukemia.With the rise of the positive expressing rate of P-gp of the leukemia cells,CsA's reverse effect on MDR is more obvious.
Objective: To explore the effect of cyclosporin A(CsA) in reversing multidrug resistance(MDR) in patients with acute leukemia(AL).Methods: Twenty-eight cases of acute leukemia were randomly divided into therapeutic group(CsA plus chemotherapy) and control group(chemotherapy alone).The expression of P-glycoprotein(P-gp) was assayed in 16 cases of the therapeutic group.Results: The total effective rate was 68.8% and the complete remission(CR) rate of the patients receiving repetition treatment was(7/10) in the therapeutic group,while the total effective rate was 25.0% and the CR rate of the patients receiving repetition treatment was 1/8 in the control group.The difference was significant(P<0.05).Conclusions: The CsA combined with chemotherapy may reverse MDR in patients with acute leukemia.With the rise of the positive expressing rate of P-gp of the leukemia cells,CsA's reverse effect on MDR is more obvious.
2006, 31(2): 150-151.
Abstract:
Objective: To investigate the surgical principle of chronic sinusitis.Methods: he effect of three surgical procedures was compared.Transmaxillary sinus ethmoidectomy(Lima's operation) was performed on 38 cases,endoscopic sinus surgery(ESS) on 31 cases and endoscopic plus Caldwell-Luc sinus surgery on 27 cases.Results: All the cases were followed up from 12 months to 24 months postoperatively.In the Lima's operation group,36.84% of the patients were cured,52.63% improved and 10.53% had no response;in the ESS group,51.61% of the patients were cured,38.71% improved and 9.68% had no response;in the endoscopic plus Caldwell-Luc sinus surgery group,77.78% of the patients were cured,14.81% improved and 7.41% failed to respond.The difference was significant.Conclusions: The approach of endoscopic plus Caldwell-Luc sinus surgery can remove the focus completely,clear the drainage thoroughly and resume the functions of nose and sinuses better.
Objective: To investigate the surgical principle of chronic sinusitis.Methods: he effect of three surgical procedures was compared.Transmaxillary sinus ethmoidectomy(Lima's operation) was performed on 38 cases,endoscopic sinus surgery(ESS) on 31 cases and endoscopic plus Caldwell-Luc sinus surgery on 27 cases.Results: All the cases were followed up from 12 months to 24 months postoperatively.In the Lima's operation group,36.84% of the patients were cured,52.63% improved and 10.53% had no response;in the ESS group,51.61% of the patients were cured,38.71% improved and 9.68% had no response;in the endoscopic plus Caldwell-Luc sinus surgery group,77.78% of the patients were cured,14.81% improved and 7.41% failed to respond.The difference was significant.Conclusions: The approach of endoscopic plus Caldwell-Luc sinus surgery can remove the focus completely,clear the drainage thoroughly and resume the functions of nose and sinuses better.
2006, 31(2): 152-153.
Abstract:
Objective: To compare the efficacy and safety of pazufloxacin in treatment of urinary tract infections.Methods: Sixty-two patients were randomly divided into two groups.The patients in therapeutic group(32 cases) were administered pazufloxcin 300 mg,intravenously drop,twice a day,while the 30 patients in control group received levofloxatin 200 mg,intravenously drop,twice a day,for 7-10 days.Results: The total effective rates of the test and the control group were 90.6% and 90.0%,the recovery rates were 84.4% and 83.3%,the bacterial clearance rates were 93.3% and 92.9%,and the adverse reaction rates were 6.3% and 6.7%,respectively.There were no significant statistical differences between the two groups(P>0.05).Conclusions: The efficacy and safety of pazufloxcin is similar to those of levofloxacin in treatment of urinary tract infections.
Objective: To compare the efficacy and safety of pazufloxacin in treatment of urinary tract infections.Methods: Sixty-two patients were randomly divided into two groups.The patients in therapeutic group(32 cases) were administered pazufloxcin 300 mg,intravenously drop,twice a day,while the 30 patients in control group received levofloxatin 200 mg,intravenously drop,twice a day,for 7-10 days.Results: The total effective rates of the test and the control group were 90.6% and 90.0%,the recovery rates were 84.4% and 83.3%,the bacterial clearance rates were 93.3% and 92.9%,and the adverse reaction rates were 6.3% and 6.7%,respectively.There were no significant statistical differences between the two groups(P>0.05).Conclusions: The efficacy and safety of pazufloxcin is similar to those of levofloxacin in treatment of urinary tract infections.
2006, 31(2): 154-156.
Abstract:
Objective: To investigate the methods and techniques in treatment of the esophagus and upper gastraintestinal tract in children with endoscopy.Methods: The data of 30 children with upper gastrointestinal tract foreign bodies between June 2000 and June 2004 were summarized retrospectively.All the cases were examined and treated with emergency endoscopy.The foreign bodies were taken out under general anesthesia or laryngopharynx local anesthesia with all kinds of medical instruments.Results: Of the 30 children(32 pieces of foreign bodies),the foreign bodies were taken out under non-general anesthesia in 28 cases and under general anesthesia in two cases.Nineteen pieces of foreign bodies were in the esophagus,9 in the stomach and 2 in the duodenum.Thirty one pieces of foreign bodies were successfully removed from 29 children,leaving no complications,bun one piece of foreign body which was located in the small intestine in one child failed to be taken out.The successful rate was 96.7%.Only one child showed transient breath inhibition and no other complications occurred in the operating process.Conclusions: Endoscopy therapy,which is simple,safe and effective should be the first choice for children patients with upper gastrointestinal tract foreign bodies.
Objective: To investigate the methods and techniques in treatment of the esophagus and upper gastraintestinal tract in children with endoscopy.Methods: The data of 30 children with upper gastrointestinal tract foreign bodies between June 2000 and June 2004 were summarized retrospectively.All the cases were examined and treated with emergency endoscopy.The foreign bodies were taken out under general anesthesia or laryngopharynx local anesthesia with all kinds of medical instruments.Results: Of the 30 children(32 pieces of foreign bodies),the foreign bodies were taken out under non-general anesthesia in 28 cases and under general anesthesia in two cases.Nineteen pieces of foreign bodies were in the esophagus,9 in the stomach and 2 in the duodenum.Thirty one pieces of foreign bodies were successfully removed from 29 children,leaving no complications,bun one piece of foreign body which was located in the small intestine in one child failed to be taken out.The successful rate was 96.7%.Only one child showed transient breath inhibition and no other complications occurred in the operating process.Conclusions: Endoscopy therapy,which is simple,safe and effective should be the first choice for children patients with upper gastrointestinal tract foreign bodies.
2006, 31(2): 156-157.
Abstract:
Objective: To study the misdiagnosis factors and operations for urethral syndrome.Methods: Three hundred and twenty-two cases(including 112 misdiagnosed cases before operation) of urethral syndrome were treated by urethral meatoplasty and the clinical data were analyzed retrospectively.Results: All the cases were followed up for 6-12 months.Two hundred and forty-one cases were cured,51 improved and 30 cases had no response.The total effective rate was 90.7%.Conclusions: The urological examination should not be neglected in the physical examination.The urethral meatoplasty is a useful and effective procedure for treatment of urethral syndrome.
Objective: To study the misdiagnosis factors and operations for urethral syndrome.Methods: Three hundred and twenty-two cases(including 112 misdiagnosed cases before operation) of urethral syndrome were treated by urethral meatoplasty and the clinical data were analyzed retrospectively.Results: All the cases were followed up for 6-12 months.Two hundred and forty-one cases were cured,51 improved and 30 cases had no response.The total effective rate was 90.7%.Conclusions: The urological examination should not be neglected in the physical examination.The urethral meatoplasty is a useful and effective procedure for treatment of urethral syndrome.
2006, 31(2): 158-159.
Abstract:
Objective: To observe the value of B-type natriuretic peptide(BNP) in differentiating cardiac dyspnea from lung diseases in children.Methods: The concentrations of BNP in 24 children with congestive heart failure(CHF),23 with pneumonia,18 with CHF combined with pneumonia and 15 healthy children were measured with BNP ELISA KIT.Results: The BNP level of the patients with CHF was(141.55±75.99)pg/ml,which was significantly higher than that of patients with pneumonia(26.00±14.57)pg/ml(P<(0.01)) and higher than that of patients with CHF combined with pneumonia(113.73±87.05)pg/ml(P<0.01),and higher than that of healthy children(19.31±10.29)pg/ml(P<0.01).The BNP level of the patients with CHF combined with pneumonia was significantly higher than that of patients with pneumonia and that of healthy children(P<0.01).The BNP level was not significantly different between the latter two groups(P>0.05).Conclusions: It suggests that the level of BNP is of value in determining the cardiac function of children with pneumonia.
Objective: To observe the value of B-type natriuretic peptide(BNP) in differentiating cardiac dyspnea from lung diseases in children.Methods: The concentrations of BNP in 24 children with congestive heart failure(CHF),23 with pneumonia,18 with CHF combined with pneumonia and 15 healthy children were measured with BNP ELISA KIT.Results: The BNP level of the patients with CHF was(141.55±75.99)pg/ml,which was significantly higher than that of patients with pneumonia(26.00±14.57)pg/ml(P<(0.01)) and higher than that of patients with CHF combined with pneumonia(113.73±87.05)pg/ml(P<0.01),and higher than that of healthy children(19.31±10.29)pg/ml(P<0.01).The BNP level of the patients with CHF combined with pneumonia was significantly higher than that of patients with pneumonia and that of healthy children(P<0.01).The BNP level was not significantly different between the latter two groups(P>0.05).Conclusions: It suggests that the level of BNP is of value in determining the cardiac function of children with pneumonia.
2006, 31(2): 160-161.
Abstract:
Objective: To explore the clinical manifestation of spontaneous subarachnoid hemorrhage presenting prominently with generalized choreiform movements.Methods: The clinical features of two spontaneous hemorrhage patients presenting prominently with generalized choreiform movements were analyzed in combination with review of the literatures.Results: The two cases were confirmed by CT scan and lumbar puncture.Conclusions: Generalized choreiform movements may be one of the main features of spontaneous subarachnoid hemorrhage.
Objective: To explore the clinical manifestation of spontaneous subarachnoid hemorrhage presenting prominently with generalized choreiform movements.Methods: The clinical features of two spontaneous hemorrhage patients presenting prominently with generalized choreiform movements were analyzed in combination with review of the literatures.Results: The two cases were confirmed by CT scan and lumbar puncture.Conclusions: Generalized choreiform movements may be one of the main features of spontaneous subarachnoid hemorrhage.
2006, 31(2): 162-164.
Abstract:
Objective: To explore the effect of pulmonary surfactant(PS) on hyaline membrane disease(HMD) of newborn.Methods: PS was administered to seventeen neonates with HMD,then the changes of clinical manifestation,the parameter of mechanical aeration,the results of blood gas and the X-ray presentations of the lung were observed.Results: After medical treatment,the symptoms and signs of the neonates were improved.The pressure peak of mechanical aeration parameter(PIP),positive breath pressure(PEEP),average gas trace pressure(MAP) and breath frequency(RR) dropped remarkably(P<0.05-P<0.01).The level of PaO2 and pH increased obviously(P<0.05 and P<0.01),and the level of PaCO2 decreased greatly(P<0.01).At 24 hours after treatment,the X-ray presentations improved significantly.Conclusions: PS therapy can improve the pathological changes of HMD,reduce the time of respirator utilization,the time of hospital stay and the fatality rate.
Objective: To explore the effect of pulmonary surfactant(PS) on hyaline membrane disease(HMD) of newborn.Methods: PS was administered to seventeen neonates with HMD,then the changes of clinical manifestation,the parameter of mechanical aeration,the results of blood gas and the X-ray presentations of the lung were observed.Results: After medical treatment,the symptoms and signs of the neonates were improved.The pressure peak of mechanical aeration parameter(PIP),positive breath pressure(PEEP),average gas trace pressure(MAP) and breath frequency(RR) dropped remarkably(P<0.05-P<0.01).The level of PaO2 and pH increased obviously(P<0.05 and P<0.01),and the level of PaCO2 decreased greatly(P<0.01).At 24 hours after treatment,the X-ray presentations improved significantly.Conclusions: PS therapy can improve the pathological changes of HMD,reduce the time of respirator utilization,the time of hospital stay and the fatality rate.
2006, 31(2): 172-174.
Abstract:
Objective: To estimate the value of spiral CT in diagnosis and treatment of aortic dissection.Methods: The imaging data of fifteen cases of aortic dissection were analyzed retrospectively.Results: On unenhanced CT scan,the imaging findings showed dilation of the aorta,linear hypoattenuating intima or intimal calcifications in the lumen and egg-like calcification on the aortic wall.On enhanced CT scan,double lumens,intimal flap,shape and location of the rupture,extension of the involved aorta and its branches were demonstrated.Conclusions: Spiral CT,especially multi-detected CT,is effective in diagnosis of aortic dissection and is of great value in guiding clinical treatment.
Objective: To estimate the value of spiral CT in diagnosis and treatment of aortic dissection.Methods: The imaging data of fifteen cases of aortic dissection were analyzed retrospectively.Results: On unenhanced CT scan,the imaging findings showed dilation of the aorta,linear hypoattenuating intima or intimal calcifications in the lumen and egg-like calcification on the aortic wall.On enhanced CT scan,double lumens,intimal flap,shape and location of the rupture,extension of the involved aorta and its branches were demonstrated.Conclusions: Spiral CT,especially multi-detected CT,is effective in diagnosis of aortic dissection and is of great value in guiding clinical treatment.
2006, 31(2): 176-177.
Abstract:
Objective: To analyze the correlation between creatinine enzyme and dry chemistry method in detection of blood plasma creatinine and to estimate the bias between the two methods.Methods: According to the guideline approved by the National Committee for Clinical Laboratory Standards(NCCLS),48 patients’ samples were analyzed in 6 operating days and each sample was analyzed in duplicate using both creatinine enzyme and dry chemistry methods.The dry chemistry method was evaluated with creatinine enzyme method acting as control.The duplicates were assessed with each method within the same run.The coefficient of correlation was calculated as well as the bias.Results: There was a significant difference between the enzyme and dry chemical method in blood creatinine determination(P<0.001),but they had a positive relativity;its linear equation was Ŷ=-3.449 4+0.929 6X and r2=(0.993 2).When the concentration of creatinine was 100 μmol/L,500 μmol/L and 900 μmol/L,the relative bias of dry chemistry method was 10.5%,7.7%,7.4%.Conclusions: The results were significantly different in detection of serum creatinine with the two different methods,but its correlation was good.The Laboratory must establish its own reference ranges for different methods or practice an emendation between the two methods.
Objective: To analyze the correlation between creatinine enzyme and dry chemistry method in detection of blood plasma creatinine and to estimate the bias between the two methods.Methods: According to the guideline approved by the National Committee for Clinical Laboratory Standards(NCCLS),48 patients’ samples were analyzed in 6 operating days and each sample was analyzed in duplicate using both creatinine enzyme and dry chemistry methods.The dry chemistry method was evaluated with creatinine enzyme method acting as control.The duplicates were assessed with each method within the same run.The coefficient of correlation was calculated as well as the bias.Results: There was a significant difference between the enzyme and dry chemical method in blood creatinine determination(P<0.001),but they had a positive relativity;its linear equation was Ŷ=-3.449 4+0.929 6X and r2=(0.993 2).When the concentration of creatinine was 100 μmol/L,500 μmol/L and 900 μmol/L,the relative bias of dry chemistry method was 10.5%,7.7%,7.4%.Conclusions: The results were significantly different in detection of serum creatinine with the two different methods,but its correlation was good.The Laboratory must establish its own reference ranges for different methods or practice an emendation between the two methods.
2006, 31(2): 178-180.
Abstract:
Objective: To study the reliability of flow cytometer UF-100 in detecting asymptomatic urinary tract infections(AUTI) in early pregnancy.Methods: Three-hundred and nine fresh midstream clean-catch urine sample were collected in accordance with standard guideline.10 μl of cleancatch midstream urine specimen was examined by cultures(colony counts of ≥ 10[4~5] CFU/ml) the other portion was tested with UF-100 for presence of bacteria count and white blood cell.Results: Twenty-one patient(6.8%) had cultures of 10,000 or more colony-forming units of a uropathogen per milliliter.The sensitivities of the UF-100 test in identifying patients with positive cultures was 71.4%,and the specificities was 94.1%.Six patients(28.6% 6/21) had negative UF-100 screening tests but positive urine cultures,and other seventeen patients had positive UF-100 screening tests but negative urine cultures,The positive predict value(PPV) of urine UF-100 evaluation was 46.9%.There was a significant difference in positive rate between the two methods(P<0.05),but there was a significant correlation in test results between the two methods(P<0.005).Conclusions: Flow cytometer UF-100 tests are not sufficiently sensitive to be of use in the screening for AUTI and therefore many patients would be missed.Bacteriuria culture must be done in diagnosing AUTI.
Objective: To study the reliability of flow cytometer UF-100 in detecting asymptomatic urinary tract infections(AUTI) in early pregnancy.Methods: Three-hundred and nine fresh midstream clean-catch urine sample were collected in accordance with standard guideline.10 μl of cleancatch midstream urine specimen was examined by cultures(colony counts of ≥ 10[4~5] CFU/ml) the other portion was tested with UF-100 for presence of bacteria count and white blood cell.Results: Twenty-one patient(6.8%) had cultures of 10,000 or more colony-forming units of a uropathogen per milliliter.The sensitivities of the UF-100 test in identifying patients with positive cultures was 71.4%,and the specificities was 94.1%.Six patients(28.6% 6/21) had negative UF-100 screening tests but positive urine cultures,and other seventeen patients had positive UF-100 screening tests but negative urine cultures,The positive predict value(PPV) of urine UF-100 evaluation was 46.9%.There was a significant difference in positive rate between the two methods(P<0.05),but there was a significant correlation in test results between the two methods(P<0.005).Conclusions: Flow cytometer UF-100 tests are not sufficiently sensitive to be of use in the screening for AUTI and therefore many patients would be missed.Bacteriuria culture must be done in diagnosing AUTI.
2006, 31(2): 180-181.
Abstract:
Objective: To observe the relationship between HCV-RNA,anti-GOR and alanine arninotransber(ALT) in patients with various hepatic diseases resulted from HCV.Serum HCV-RNA,anti-GOR and ALT levels of 69 cases of various hepatic diseases with anti-HCV positive were measured and compared.Methods: Anti-HCV and anti-GOR were measured by ELISA and HCVRNA was determined by PCR.Results: The positive rates of HCV-RNA and anti-GOR were 59.4%,61.9%,56.3% and 71.9% 66.7%,(68.8%) respectively in the patients with chronic hepatitis C,hepatocirrhosis,and hepatocellular carcinoma.The positive rate of anti-GOR was significantly higher in the ALT>40 u/L group than that in the ALT ≤ 40 u/L group(P<0.005 and P<0.05).Conclusions: Detection of anti-GOR is of value for the diagnosis of HCV.The positive rate of anti-GOR correlates with the activity of ALT.
Objective: To observe the relationship between HCV-RNA,anti-GOR and alanine arninotransber(ALT) in patients with various hepatic diseases resulted from HCV.Serum HCV-RNA,anti-GOR and ALT levels of 69 cases of various hepatic diseases with anti-HCV positive were measured and compared.Methods: Anti-HCV and anti-GOR were measured by ELISA and HCVRNA was determined by PCR.Results: The positive rates of HCV-RNA and anti-GOR were 59.4%,61.9%,56.3% and 71.9% 66.7%,(68.8%) respectively in the patients with chronic hepatitis C,hepatocirrhosis,and hepatocellular carcinoma.The positive rate of anti-GOR was significantly higher in the ALT>40 u/L group than that in the ALT ≤ 40 u/L group(P<0.005 and P<0.05).Conclusions: Detection of anti-GOR is of value for the diagnosis of HCV.The positive rate of anti-GOR correlates with the activity of ALT.
2006, 31(2): 182-184.
Abstract:
Objective: To study the variation of soluble serum Fas and soluble Fas Ligand in patients with aplastic anemia.Methods: The level of sFas and sFas Ligand in the serum of 30 patients with aplastic anemia were measured by enzyme-linked immunosorbent assay(ELISA).Results: The level of sFas in the patients with aplastic anemia(AA) was apparently lower than that of normal controls(P<0.01),and the level of sFas in the patients with severe aplastic anemia(SAA)was much lower than that of patients with chronic aplastic anemia(CAA)(P<0.05);The level of sFas in AA patients was related to the clinical types and curative effects.The levels of sFas ligand in AA patients and controls had no difference.Conclusions: The level of sFas in AA patients is below the normal.Detection of the level of sFas in AA patients is an effective indication of the condition of the patients.
Objective: To study the variation of soluble serum Fas and soluble Fas Ligand in patients with aplastic anemia.Methods: The level of sFas and sFas Ligand in the serum of 30 patients with aplastic anemia were measured by enzyme-linked immunosorbent assay(ELISA).Results: The level of sFas in the patients with aplastic anemia(AA) was apparently lower than that of normal controls(P<0.01),and the level of sFas in the patients with severe aplastic anemia(SAA)was much lower than that of patients with chronic aplastic anemia(CAA)(P<0.05);The level of sFas in AA patients was related to the clinical types and curative effects.The levels of sFas ligand in AA patients and controls had no difference.Conclusions: The level of sFas in AA patients is below the normal.Detection of the level of sFas in AA patients is an effective indication of the condition of the patients.
2006, 31(2): 190-192.
Abstract:
Objective: To analyze the correlative factors of ocular trauma and to take effective nursing measures to improve the vision and reduce the rate of blindness.Methods: A retrospective clinical analysis of correlative factors of ocular trauma was performed on 132 admission cases(135 eyes) in 2004 and to find out the points of nursing care.Results: The patients with ocular trauma accounted for 16.79% of the admission cases in the same period.Most of the ocular trauma patients were children,students and the youth.The patients’occupations were mainly students,farmers and no-jobs.Fifty-six(42.42%) cases went to hospital within 24 hours after injury and 76(57.58%) cases after 24 hours.Blindness occurred in 29 eyes,with a blindness rate of 21.48%.Conclusions: Preventive measures,first-aid and health education on nursing care should be strengthened and the patients should also have self-protective consciousness so as to reduce the rate of ocular trauma and blindness.
Objective: To analyze the correlative factors of ocular trauma and to take effective nursing measures to improve the vision and reduce the rate of blindness.Methods: A retrospective clinical analysis of correlative factors of ocular trauma was performed on 132 admission cases(135 eyes) in 2004 and to find out the points of nursing care.Results: The patients with ocular trauma accounted for 16.79% of the admission cases in the same period.Most of the ocular trauma patients were children,students and the youth.The patients’occupations were mainly students,farmers and no-jobs.Fifty-six(42.42%) cases went to hospital within 24 hours after injury and 76(57.58%) cases after 24 hours.Blindness occurred in 29 eyes,with a blindness rate of 21.48%.Conclusions: Preventive measures,first-aid and health education on nursing care should be strengthened and the patients should also have self-protective consciousness so as to reduce the rate of ocular trauma and blindness.
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