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2006 Vol. 31, No. 3
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2006, 31(3): 226-229.
Abstract:
Objective: To explore the biological activities of viral macrophage inflammatory protein(vMIP) encoded by K6 gene of human herpesvirus 8.Methods: Combined ability of vMIP to receptors was measured by Cross-linking assays.Biological activity of vMIP were measured with Cellular chemotaxis assays and calcium mobilization.Results: Cross-linking assays showed that vMIP bound with receptors of peripheral blood mononuclear,and vMIP suppressed by chemotacitic activity of PBMCs to human macrophage inflammatory protein(hMIP-1α) and EC50=3.39 ng/ml.But itself was not remarkably associated with the normal,rapid mobilization of calcium from intracellular stores.Instead,it blocked calcium mobilization induced by endogenous chemokines.Conclusions: The study has demonstrated that vMIP can bind to receptor of hMIP-1(CCR5) and stop it from hMIP-1 and conduction of messages.But itself did not induce cellular chemotaxis,meaning that vMIP has ability of binding to CCR5 similar hMIP-1α and absent of function activating receptor like hMIP-1α,so vMIP only has sealing effect on CCR5.This also suggests that only of effects of vMIP is to seal CCR5 of host cells,vMIP is the natural sealing factor for CCR5 and result in inhibiting inflammatory reaction mediated by interaction between CCR5 and human MIPs,so possibly it is important value in prevention and treatment of inflammation,transplantion immunity and HIV infection.
Objective: To explore the biological activities of viral macrophage inflammatory protein(vMIP) encoded by K6 gene of human herpesvirus 8.Methods: Combined ability of vMIP to receptors was measured by Cross-linking assays.Biological activity of vMIP were measured with Cellular chemotaxis assays and calcium mobilization.Results: Cross-linking assays showed that vMIP bound with receptors of peripheral blood mononuclear,and vMIP suppressed by chemotacitic activity of PBMCs to human macrophage inflammatory protein(hMIP-1α) and EC50=3.39 ng/ml.But itself was not remarkably associated with the normal,rapid mobilization of calcium from intracellular stores.Instead,it blocked calcium mobilization induced by endogenous chemokines.Conclusions: The study has demonstrated that vMIP can bind to receptor of hMIP-1(CCR5) and stop it from hMIP-1 and conduction of messages.But itself did not induce cellular chemotaxis,meaning that vMIP has ability of binding to CCR5 similar hMIP-1α and absent of function activating receptor like hMIP-1α,so vMIP only has sealing effect on CCR5.This also suggests that only of effects of vMIP is to seal CCR5 of host cells,vMIP is the natural sealing factor for CCR5 and result in inhibiting inflammatory reaction mediated by interaction between CCR5 and human MIPs,so possibly it is important value in prevention and treatment of inflammation,transplantion immunity and HIV infection.
2006, 31(3): 230-232.
Abstract:
Objective: To investigate the effect of protein kinase C (PKC) on the apoptosis of human γδT cells induced by ethanol.Methods: The peripheral blood mononuclear cells of healthy donors were stimulated with the peptide antigen derived from Mycobacterium tuberculosis antigen (Mtb-Ag),to generate Mtb-Ag activated T cells (γδT cells were predominant).The apoptosis of human γδT cells induced by different concentrations of ethanol was measured by flowcytometry using Annexin-V-FITC/PI staining,and the effect of pretreatment with the PKC inhibitor Rottlerin on the apoptosis of human γδT cells induced by ethanol was also investigated.Results: The apoptosis of activated human γδT cells stimulated with Mtb-Ag were significantly induced by treatment of γδT ceils with 4.0% ethanol for 4,12 and 24 hours (P<0.01).The apoptosis of γδT cells induced by 4.0% ethanol were inhibited remarkably by 5.0 μmol/L and 40.0 μmol/L Rottlerin,the inhibitory rates of apoptosis were 75.17% and 58.95%(P<0.01),respectively.Conclusions: The results suggested the PKC signaling pathways participated in the apoptosis of γδT cells induced by ethanol.
Objective: To investigate the effect of protein kinase C (PKC) on the apoptosis of human γδT cells induced by ethanol.Methods: The peripheral blood mononuclear cells of healthy donors were stimulated with the peptide antigen derived from Mycobacterium tuberculosis antigen (Mtb-Ag),to generate Mtb-Ag activated T cells (γδT cells were predominant).The apoptosis of human γδT cells induced by different concentrations of ethanol was measured by flowcytometry using Annexin-V-FITC/PI staining,and the effect of pretreatment with the PKC inhibitor Rottlerin on the apoptosis of human γδT cells induced by ethanol was also investigated.Results: The apoptosis of activated human γδT cells stimulated with Mtb-Ag were significantly induced by treatment of γδT ceils with 4.0% ethanol for 4,12 and 24 hours (P<0.01).The apoptosis of γδT cells induced by 4.0% ethanol were inhibited remarkably by 5.0 μmol/L and 40.0 μmol/L Rottlerin,the inhibitory rates of apoptosis were 75.17% and 58.95%(P<0.01),respectively.Conclusions: The results suggested the PKC signaling pathways participated in the apoptosis of γδT cells induced by ethanol.
2006, 31(3): 233-234.
Abstract:
Objective: To study the effect of arsenic trioxide on apoptosis of melanoma B16 cells.Methods: Early apoptosis rate,late apoptosis rate and total apoptosis rate in melanoma B16 cells were analysed by flow cytometry.Results: At the doses of arsenic trioxide were 5 μmol/L,10 μmol/L,or 20 μmol/L,the early apoptosis rates in melanoma B16 cells were 7.18%,21.1%,and 3.59%,respectively;the late apoptosis rates were 2.63%,5.35%,and 6.34%,respectively;the total apoptosis rates were 9.81%,26.45%,and 9.93%,respectively.Conclusions: The early apoptosis rate and the late apoptosis rate in melanoma B16 cells have dose-dependent relationship with arsenic trioxide.
Objective: To study the effect of arsenic trioxide on apoptosis of melanoma B16 cells.Methods: Early apoptosis rate,late apoptosis rate and total apoptosis rate in melanoma B16 cells were analysed by flow cytometry.Results: At the doses of arsenic trioxide were 5 μmol/L,10 μmol/L,or 20 μmol/L,the early apoptosis rates in melanoma B16 cells were 7.18%,21.1%,and 3.59%,respectively;the late apoptosis rates were 2.63%,5.35%,and 6.34%,respectively;the total apoptosis rates were 9.81%,26.45%,and 9.93%,respectively.Conclusions: The early apoptosis rate and the late apoptosis rate in melanoma B16 cells have dose-dependent relationship with arsenic trioxide.
2006, 31(3): 235-237.
Abstract:
Objective: To investigate the role of tumor necrosis factor-α(TNF-α) in liver damage in rats with obstructive jaundice and the protective effect of anti-TNF-αMcAb.Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups:bile duct-ligation(BDL) group,bile duct-ligation+anti-TNF-αMcAb(BDL+anti-TNF-αMcAb) group and sham-operation(SO) group.BDL+anti-TNF-αMcAb group was subjected to damage with intravenous administration of anti-TNF-αMcAb(1mg/kg) for five days,starting from the 3rd day after bile duct-ligation;BDL group and SO group were administered with saline in the same manner.The levels of serum alanine amiotransferase(ALT),aspartate aminotransferase(AST) and TNF-α were detected in each group.The histopathology was observed by light and electron microscopy in the liver of rats with obstructive jaundice.Results: The levels of serum ALT,AST and TNF-α in BDL group and BDL+anti-TNF-αMcAb group were markedly higher than those in SO group(P<0.01),and the levels of serum ALT,AST and TNF-α in BDL+anti-TNF-αMcAb group were much lower than those in BDL group(P<0.05 to P<0.01),the changes of liver histopathology were minor in BDL+anti-TNF-αMcAb group.SO group had no apparent change.Conclusions: TNF-α may be one of the most important mediators in live damage induced by obstructive jaundice,and the use of anti-TNF-αMcAb can relieve the liver damage.
Objective: To investigate the role of tumor necrosis factor-α(TNF-α) in liver damage in rats with obstructive jaundice and the protective effect of anti-TNF-αMcAb.Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups:bile duct-ligation(BDL) group,bile duct-ligation+anti-TNF-αMcAb(BDL+anti-TNF-αMcAb) group and sham-operation(SO) group.BDL+anti-TNF-αMcAb group was subjected to damage with intravenous administration of anti-TNF-αMcAb(1mg/kg) for five days,starting from the 3rd day after bile duct-ligation;BDL group and SO group were administered with saline in the same manner.The levels of serum alanine amiotransferase(ALT),aspartate aminotransferase(AST) and TNF-α were detected in each group.The histopathology was observed by light and electron microscopy in the liver of rats with obstructive jaundice.Results: The levels of serum ALT,AST and TNF-α in BDL group and BDL+anti-TNF-αMcAb group were markedly higher than those in SO group(P<0.01),and the levels of serum ALT,AST and TNF-α in BDL+anti-TNF-αMcAb group were much lower than those in BDL group(P<0.05 to P<0.01),the changes of liver histopathology were minor in BDL+anti-TNF-αMcAb group.SO group had no apparent change.Conclusions: TNF-α may be one of the most important mediators in live damage induced by obstructive jaundice,and the use of anti-TNF-αMcAb can relieve the liver damage.
2006, 31(3): 238-240.
Abstract:
Objective: To study the effects of taurine on diaphragm injury in diabetic rats.Methods: Twenty-four SD rats were randomly divided into normal control(NC) group,diabetic(DM) group and taurine treatment(Tau) group.DM group and Tau group rats were given streptozocin(STZ) 50 mg/kg intraperitoneal injection.NC group and DM group rats were treated with watar,Tau group rats were treated with 1% taurine in drinking watar.After 4 weeks,the body weight(BW) and level of blood glucose(BG) were measured.Superoxide dismutase(SOD),nitric oxide synthase(NOS) activities and malondialdehyde(MDA),NO contents in diaphragm tissues were also measured.The ultrastructure of diaphragm were intvestigated by electron microscope.Results: SOD,NOS activities,NO contents were significantly lower(P<0.01),BG and MDA content were significantly higher in DM group(P<0.01).Compare with DM group,SOD,NOS activities and NO contents were higher(P<0.05 and P<0.01),BG and MDA content were lower in Tau group(P<0.01).The ultrastructure under electron microscope indicated that in DM group diaphragm fibres fracture,mitochondrions swelling and degeneration.The above changes were inhibited by treatment of taurine.Conclusions: Taurine can increase SOD,NOS activities,NO contents and decrease MDA conten in diabetic diaphragm.
Objective: To study the effects of taurine on diaphragm injury in diabetic rats.Methods: Twenty-four SD rats were randomly divided into normal control(NC) group,diabetic(DM) group and taurine treatment(Tau) group.DM group and Tau group rats were given streptozocin(STZ) 50 mg/kg intraperitoneal injection.NC group and DM group rats were treated with watar,Tau group rats were treated with 1% taurine in drinking watar.After 4 weeks,the body weight(BW) and level of blood glucose(BG) were measured.Superoxide dismutase(SOD),nitric oxide synthase(NOS) activities and malondialdehyde(MDA),NO contents in diaphragm tissues were also measured.The ultrastructure of diaphragm were intvestigated by electron microscope.Results: SOD,NOS activities,NO contents were significantly lower(P<0.01),BG and MDA content were significantly higher in DM group(P<0.01).Compare with DM group,SOD,NOS activities and NO contents were higher(P<0.05 and P<0.01),BG and MDA content were lower in Tau group(P<0.01).The ultrastructure under electron microscope indicated that in DM group diaphragm fibres fracture,mitochondrions swelling and degeneration.The above changes were inhibited by treatment of taurine.Conclusions: Taurine can increase SOD,NOS activities,NO contents and decrease MDA conten in diabetic diaphragm.
2006, 31(3): 241-242.
Abstract:
Objective: To probe the cytogenetic base on male infertility patients.Methods: The chromosome karyotypes of 266 male infertility patients were analyzed by cytogenetic method.Results: Among 266 cases,66 cases(24.81% of total patients) showed abnormal karyotypes,including 52 cases(78.79% of totality) of Klinefelter syndrome,6 cases of Yqh+,6 cases of chromosome structure abnormality,one case of 46,XY,del(y),(q11) and one case of 46,XX male syndrome.Conclusions: Chromosome abnormality is an important cause in male infertility,which has serious effect on the growth of male.Klinefelter syndrome is the main type of chromosome abnormality in male infertility.Comparing with others,its symptom is very typical.
Objective: To probe the cytogenetic base on male infertility patients.Methods: The chromosome karyotypes of 266 male infertility patients were analyzed by cytogenetic method.Results: Among 266 cases,66 cases(24.81% of total patients) showed abnormal karyotypes,including 52 cases(78.79% of totality) of Klinefelter syndrome,6 cases of Yqh+,6 cases of chromosome structure abnormality,one case of 46,XY,del(y),(q11) and one case of 46,XX male syndrome.Conclusions: Chromosome abnormality is an important cause in male infertility,which has serious effect on the growth of male.Klinefelter syndrome is the main type of chromosome abnormality in male infertility.Comparing with others,its symptom is very typical.
2006, 31(3): 243-245.
Abstract:
Objective: To investigate the association between the genes of human leukocyte antigens(HLA)-DR1-DR10 and the clinical features,stages by X-ray image as well as the laboratory test results of rheumatoid arthritis(RA) in the population of Han nationality in northern Anhui province.Methods: The method of amplification with polymerase chain reaction-sequence specific primer(PCR-SSP) was used to determine the HLA-DR1-DR10 gene types in 80 patients with RA and 40 healthy controls from Han nationality in northern Anhui.The association between the gene types of HLA-DR and the clinical features,X-ray stages of articulations and the laboratory test results were analyzed.Results: The frequency of HLA-DR4 alleles was 35% in the RA patients and 15% in the healthy controls.The frequency of HLA-DR4 alleles was significantly higher in RA patients than that in normal subjects(P<0.05).The frequency of HLA-DR4 alleles was higher in the group with positive sera of the rheumatoid factor(RF) and subsequent lung diseases(P<0.05 and P<0.005).The level of RF,anti-CCP antibody and erythrocyte sedimentation rate(ESR)in patients carrying HLA-DR4 gene was significantly higher than that of other patients(P<0.05-P<0.01).The time of morning stiffness was longer in the patients with HLA-DR4 allele positive(P<0.001).The function and X-ray staging of articulations of class Ⅲ or Ⅳ were more frequently seen in patients with HLA-DR4 positive than in others(P<0.01).Conclusions: The susceptibility of RA is related to HLA-DR4 allele in the population of Han nationality in northern Anhui.HLA-DR4 allele may be related with the time of morning stiffness,level of RF and anti-CCP antibody,ESR and the degree of sclerotic erosion.Patients with HLA-DR4 positive are more liable to acquire subsequent lung injury.
Objective: To investigate the association between the genes of human leukocyte antigens(HLA)-DR1-DR10 and the clinical features,stages by X-ray image as well as the laboratory test results of rheumatoid arthritis(RA) in the population of Han nationality in northern Anhui province.Methods: The method of amplification with polymerase chain reaction-sequence specific primer(PCR-SSP) was used to determine the HLA-DR1-DR10 gene types in 80 patients with RA and 40 healthy controls from Han nationality in northern Anhui.The association between the gene types of HLA-DR and the clinical features,X-ray stages of articulations and the laboratory test results were analyzed.Results: The frequency of HLA-DR4 alleles was 35% in the RA patients and 15% in the healthy controls.The frequency of HLA-DR4 alleles was significantly higher in RA patients than that in normal subjects(P<0.05).The frequency of HLA-DR4 alleles was higher in the group with positive sera of the rheumatoid factor(RF) and subsequent lung diseases(P<0.05 and P<0.005).The level of RF,anti-CCP antibody and erythrocyte sedimentation rate(ESR)in patients carrying HLA-DR4 gene was significantly higher than that of other patients(P<0.05-P<0.01).The time of morning stiffness was longer in the patients with HLA-DR4 allele positive(P<0.001).The function and X-ray staging of articulations of class Ⅲ or Ⅳ were more frequently seen in patients with HLA-DR4 positive than in others(P<0.01).Conclusions: The susceptibility of RA is related to HLA-DR4 allele in the population of Han nationality in northern Anhui.HLA-DR4 allele may be related with the time of morning stiffness,level of RF and anti-CCP antibody,ESR and the degree of sclerotic erosion.Patients with HLA-DR4 positive are more liable to acquire subsequent lung injury.
2006, 31(3): 246-248.
Abstract:
Objective: To investigate the changes of hemodynamics and homeostasis during orthotopic liver transplantation and the anesthetic management.Methods: Fourteen patients with the chronic end-stage liver disease underwent orthotopic liver transplantation under combined intravenous-inhalational anesthesia.The Swan-Ganz tube was inserted to the right ventricle to observe the changes of hemodynamics and homeostasis at different stages.Results: All the patients received the operation smoothly.At anhepatic stage,pulmonary arterial wedge pressure(PAWP),mean systemic arterial pressure(MAP) and central venous pressure(CVP) decreased significantly compared with those before operation(P<0.01),but at the point of unclamping vena cava,PAWP and CVP increased significantly,while MAP decreased(P<0.01).Mean pulmonary arterial pressure(MPAP) kept at a high level at the end of operation(P<0.01).cardiac output(CO) was lower at the point of clamping or unclamping vena cava than before operation(P<0.05).Conclusions: The changes of homodynamics mainly occur at anhepatic and early neohepatic stage.Comprehensive treatment and timely management may check the changes.
Objective: To investigate the changes of hemodynamics and homeostasis during orthotopic liver transplantation and the anesthetic management.Methods: Fourteen patients with the chronic end-stage liver disease underwent orthotopic liver transplantation under combined intravenous-inhalational anesthesia.The Swan-Ganz tube was inserted to the right ventricle to observe the changes of hemodynamics and homeostasis at different stages.Results: All the patients received the operation smoothly.At anhepatic stage,pulmonary arterial wedge pressure(PAWP),mean systemic arterial pressure(MAP) and central venous pressure(CVP) decreased significantly compared with those before operation(P<0.01),but at the point of unclamping vena cava,PAWP and CVP increased significantly,while MAP decreased(P<0.01).Mean pulmonary arterial pressure(MPAP) kept at a high level at the end of operation(P<0.01).cardiac output(CO) was lower at the point of clamping or unclamping vena cava than before operation(P<0.05).Conclusions: The changes of homodynamics mainly occur at anhepatic and early neohepatic stage.Comprehensive treatment and timely management may check the changes.
2006, 31(3): 249-250.
Abstract:
Objective: To observe the effect and safety of intravesical instillation of hydroxycamptothecin(HCPT) associated with pirarubicin(THP) for preventing postoperative recurrence of the superficial bladder cancer.Methods: The intravesical instillation of HCPT associated with THP was performed in 32 patients with bladder cancer after operation and till two years after operation.The local and overall reaction was recorded for each instillation.The cystoscopy was performed once every three months.Results: All the patients were followed up for 9 up to 50 months with 20 months on everage.Recurrence occurred in two patients(6.3%).The aforementioned therapy was tolerated by all patients.Conclusions: The intravesical instillation of HCPT associated with THP is an effective and safe treatment for superficial bladder cancer with good tolerance,less toxic and adverse effects and satisfactory results.
Objective: To observe the effect and safety of intravesical instillation of hydroxycamptothecin(HCPT) associated with pirarubicin(THP) for preventing postoperative recurrence of the superficial bladder cancer.Methods: The intravesical instillation of HCPT associated with THP was performed in 32 patients with bladder cancer after operation and till two years after operation.The local and overall reaction was recorded for each instillation.The cystoscopy was performed once every three months.Results: All the patients were followed up for 9 up to 50 months with 20 months on everage.Recurrence occurred in two patients(6.3%).The aforementioned therapy was tolerated by all patients.Conclusions: The intravesical instillation of HCPT associated with THP is an effective and safe treatment for superficial bladder cancer with good tolerance,less toxic and adverse effects and satisfactory results.
2006, 31(3): 251-253.
Abstract:
Objective: To investigate the correlation between sleep apnea syndrome(SAS) and the age and body weight.Methods: One hundred and sixteen patients with SAS were diagnosed by polysomnography(PSG) and classified into the aged group and middle-aged group,the obesity group and non-obesity group.Results: The apnea hypopnea index(AHI),longest apnea time(LAT) and mean apnea time(MAT) of the obesity group were significantly higher than those of the non-obesity group,and the index of lowest arterial O2 saturation(LSaO2) and mean arterial O2 saturation(MSaO2) were lower than those of the non-obesity group(P<0.005 to P<0.001).Both the body mass index(BMI) and neck circumference were lower in the aged group than in the middle-aged group(P<0.01 to P<0.005);AHI,LAT and MAT were lower in the aged group comparatively.LSaO2 and MSaO2 were significantly higher in the aged group than those in the middle-aged group(P<0.05 to P<0.005).Conclusions: The severity of SAS is positively correlated with body weight and negatively correlated with age.
Objective: To investigate the correlation between sleep apnea syndrome(SAS) and the age and body weight.Methods: One hundred and sixteen patients with SAS were diagnosed by polysomnography(PSG) and classified into the aged group and middle-aged group,the obesity group and non-obesity group.Results: The apnea hypopnea index(AHI),longest apnea time(LAT) and mean apnea time(MAT) of the obesity group were significantly higher than those of the non-obesity group,and the index of lowest arterial O2 saturation(LSaO2) and mean arterial O2 saturation(MSaO2) were lower than those of the non-obesity group(P<0.005 to P<0.001).Both the body mass index(BMI) and neck circumference were lower in the aged group than in the middle-aged group(P<0.01 to P<0.005);AHI,LAT and MAT were lower in the aged group comparatively.LSaO2 and MSaO2 were significantly higher in the aged group than those in the middle-aged group(P<0.05 to P<0.005).Conclusions: The severity of SAS is positively correlated with body weight and negatively correlated with age.
2006, 31(3): 253-254.
Abstract:
Objective: To investigate the application of symmetric triangular flap in repairing incomplete unilateral cleft lips.Methods: Thirty cases of incomplete unilateral cleft lip were repaired with symmetric triangular flap.A vermilion triangular flap was designed in the affected side and inserted into the other side to repair the vermilion.Results: All the thirty cases achieved first phase healing,with symmetry nasal ala,satisfactory shape of the upper lip and inconspicuous scar.Conclusions: The method is rational and simple,and is easy to master.The shape of the upper lip and nose is satisfactory after repairing.
Objective: To investigate the application of symmetric triangular flap in repairing incomplete unilateral cleft lips.Methods: Thirty cases of incomplete unilateral cleft lip were repaired with symmetric triangular flap.A vermilion triangular flap was designed in the affected side and inserted into the other side to repair the vermilion.Results: All the thirty cases achieved first phase healing,with symmetry nasal ala,satisfactory shape of the upper lip and inconspicuous scar.Conclusions: The method is rational and simple,and is easy to master.The shape of the upper lip and nose is satisfactory after repairing.
2006, 31(3): 255-257.
Abstract:
Objective: To investigate the sensitivity and specificity of anti-SmD1 antibody in diagnosis of systemic lupus erythematosus and the relationship between the level of antibody in sera and the activity of systemic lupus erythematosus(SLE).Methods: The anti-SmD1 antibody in 137 cases with SLE and 75 cases without SLE were measured by line immuno assay(LIA).The cases with positive results were determined by ELISA again.The specificity and sensitvity of anti-SmD1 antibody in sera of the patients were analyzed and the relations between the clinical manifestations,examination results and the active score of the patients were observed.Results: The sensitivity of anti-SmD1 antibody by LIA was 84.9% and the specificity was 96.0%.The level of anti-SmD1 antibody was positively related with SLEDMI(r=0.674,P<0.01).Conclusions: The sensitivity by LIA is significantly higher than that by IBT.The level of anti-SmD1 antibody in sera is associated with the activity of SLE.
Objective: To investigate the sensitivity and specificity of anti-SmD1 antibody in diagnosis of systemic lupus erythematosus and the relationship between the level of antibody in sera and the activity of systemic lupus erythematosus(SLE).Methods: The anti-SmD1 antibody in 137 cases with SLE and 75 cases without SLE were measured by line immuno assay(LIA).The cases with positive results were determined by ELISA again.The specificity and sensitvity of anti-SmD1 antibody in sera of the patients were analyzed and the relations between the clinical manifestations,examination results and the active score of the patients were observed.Results: The sensitivity of anti-SmD1 antibody by LIA was 84.9% and the specificity was 96.0%.The level of anti-SmD1 antibody was positively related with SLEDMI(r=0.674,P<0.01).Conclusions: The sensitivity by LIA is significantly higher than that by IBT.The level of anti-SmD1 antibody in sera is associated with the activity of SLE.
2006, 31(3): 257-258.
Abstract:
Objective: To investigate the therapeutic efficacy of triamcinolone injection through peripheral nerve trunk on trigeminal neuralgia.Methods: Eighty-six cases suffering from trigeminal neuralgia were injected triamcinolone through the peripheral nerve trunk in foramen rotundun and foramen ovale once a week for five weeks.Results: The pain disappeared in 70 cases and was relived in 14 cases after treatment.The remission rate was 97.6%.Seventy cases were followed up for one year,and 6 cases recurred,with a recurrence rate of 8.6%;sixty cases were followed for three-years and 18 cases recurred,with a recurrence rate of 30%.Conclusions: The therapy of triamcinolone is simple,safe and effective for treatment of trigenminal neuraligia.
Objective: To investigate the therapeutic efficacy of triamcinolone injection through peripheral nerve trunk on trigeminal neuralgia.Methods: Eighty-six cases suffering from trigeminal neuralgia were injected triamcinolone through the peripheral nerve trunk in foramen rotundun and foramen ovale once a week for five weeks.Results: The pain disappeared in 70 cases and was relived in 14 cases after treatment.The remission rate was 97.6%.Seventy cases were followed up for one year,and 6 cases recurred,with a recurrence rate of 8.6%;sixty cases were followed for three-years and 18 cases recurred,with a recurrence rate of 30%.Conclusions: The therapy of triamcinolone is simple,safe and effective for treatment of trigenminal neuraligia.
2006, 31(3): 259-261.
Abstract:
Objective: To review the perioperative management for tetralogy of fallot(TOF).Methods: Seventeen patients with tetralogy of fallot received surgical treatments,and the clinical data,preoperative heart function and surgical results were reviewed and analysed.Results: There was no early or late death,and no myocardical infarction.The follow-up ranged from 3 months to half a year.No residual leakage or obstruction of right ventricle outflow tract occurred.Conclusions: Proper selection of indications,early detection and appropriate management of postoperative complication are essential to the surgical outcome.
Objective: To review the perioperative management for tetralogy of fallot(TOF).Methods: Seventeen patients with tetralogy of fallot received surgical treatments,and the clinical data,preoperative heart function and surgical results were reviewed and analysed.Results: There was no early or late death,and no myocardical infarction.The follow-up ranged from 3 months to half a year.No residual leakage or obstruction of right ventricle outflow tract occurred.Conclusions: Proper selection of indications,early detection and appropriate management of postoperative complication are essential to the surgical outcome.
2006, 31(3): 261-262.
Abstract:
Objective: To investigate the rational scope of resection in treatment of the upper third gastric cancer.Methods: The clinical data of 191 patients undergoing radical resection for upper third gastric adenocarcinoma were analyzed and the efficacy of lymphadenectomy for the groups of the lymph nodes were evaluated.Results: The index of efficacy was the highest for lymph node removal in the paracardial regions,followed by along the left gastric artery,along the pancreatic artery,along the left renal vein and along the abdominal aorta.Removal of the lateroaortic lymph node on the cranial side of the left renal vein had a relatively ligh efficacy index.In patients with the esophagus involved,the efficacy of lower paraesophageal and supraphrenic lymph node removal was high.Conclusions: The scope of lymphadenectomy should be tailored to tumor location.The lymphadenectomy might include the lateroaortic lymph nodes for advanced gastric cancer in the upper third of the stomach and the supradiaphragmatic and lower paraesophageal nodes for tumors extending to the esophagus.
Objective: To investigate the rational scope of resection in treatment of the upper third gastric cancer.Methods: The clinical data of 191 patients undergoing radical resection for upper third gastric adenocarcinoma were analyzed and the efficacy of lymphadenectomy for the groups of the lymph nodes were evaluated.Results: The index of efficacy was the highest for lymph node removal in the paracardial regions,followed by along the left gastric artery,along the pancreatic artery,along the left renal vein and along the abdominal aorta.Removal of the lateroaortic lymph node on the cranial side of the left renal vein had a relatively ligh efficacy index.In patients with the esophagus involved,the efficacy of lower paraesophageal and supraphrenic lymph node removal was high.Conclusions: The scope of lymphadenectomy should be tailored to tumor location.The lymphadenectomy might include the lateroaortic lymph nodes for advanced gastric cancer in the upper third of the stomach and the supradiaphragmatic and lower paraesophageal nodes for tumors extending to the esophagus.
2006, 31(3): 263-265.
Abstract:
Objective: To explore the expression of vascular endothelial growth factor(VEGF) and its relationship with progression of human brain glioma.Methods: VEGF protein was detected by immunohistochemistry in 60 cases of glioma and 10 cases of normal brain tissues.Nucleic acid hybridization in situ was used to examine the expression of VEGF mRNA in the tissues.Results: The positive expression of VEGF protein and VEGF mRNA increased significantly with the pathological grades of glioma(P<0.01).There was no expression in the normal human brain.Conclusions: The high expression of VEGF is obviously related to the progression of human glioma.VEGF may contribute to the progression of human glioma by playing a part in the formation of angiogenesis.
Objective: To explore the expression of vascular endothelial growth factor(VEGF) and its relationship with progression of human brain glioma.Methods: VEGF protein was detected by immunohistochemistry in 60 cases of glioma and 10 cases of normal brain tissues.Nucleic acid hybridization in situ was used to examine the expression of VEGF mRNA in the tissues.Results: The positive expression of VEGF protein and VEGF mRNA increased significantly with the pathological grades of glioma(P<0.01).There was no expression in the normal human brain.Conclusions: The high expression of VEGF is obviously related to the progression of human glioma.VEGF may contribute to the progression of human glioma by playing a part in the formation of angiogenesis.
2006, 31(3): 265-266.
Abstract:
Objective: To observe the change of thyroid hormone in the serum of patients with chronic obstructive pulmonary disease(COPD) at the acute stage and the stationary phase.Methods: The levels of T3,FT3,T4,FT4 and TSH in the serum of 39 patients with COPD were detected before treatment and two weeks after treatment,and the results were compared with those of the controls.Results: The levels of T3,FT3,T4 in the serum of patients with COPD were lower than those of the controls(P<0.01).After treatment,the above levels decreased significantly in 36 cases(P<0.01),but did not in 3 severe cases.Conclusions: The dynamic change of thyroid hormone in the serum of patients with COPD is of value for the judgment of the prognosis of the patients.
Objective: To observe the change of thyroid hormone in the serum of patients with chronic obstructive pulmonary disease(COPD) at the acute stage and the stationary phase.Methods: The levels of T3,FT3,T4,FT4 and TSH in the serum of 39 patients with COPD were detected before treatment and two weeks after treatment,and the results were compared with those of the controls.Results: The levels of T3,FT3,T4 in the serum of patients with COPD were lower than those of the controls(P<0.01).After treatment,the above levels decreased significantly in 36 cases(P<0.01),but did not in 3 severe cases.Conclusions: The dynamic change of thyroid hormone in the serum of patients with COPD is of value for the judgment of the prognosis of the patients.
2006, 31(3): 267-268.
Abstract:
Objective: To observe the influence of trial vaginal delivery in women with cord around neck foetus on the outcome of perinatal infants.Methods: Sevety-six cases with cord around neck foetus undergoing trial vaginal delivery and 111 cases controls were analyzed retrospectively.Results: The incidence of fetal distress in cases with cord around neck foetus in vaginal delivery was no significant difference compared with the control group(P<0.05),wheareas the incidence of fetal distress in foetus with cord around neck for 2 week was significantly higher than that in case with cord around neck for 1 week.The incidence of asphyxia and difficult labor in the two groups had no significant difference(P>0.05).Conclusions: The parturient with cord around neck feotus should be given trial vaginal delivery.Close monitoring in delivery and timely and effective treatment may reduce the incidence of cesarean section and improve the outcome of perinatal infants.
Objective: To observe the influence of trial vaginal delivery in women with cord around neck foetus on the outcome of perinatal infants.Methods: Sevety-six cases with cord around neck foetus undergoing trial vaginal delivery and 111 cases controls were analyzed retrospectively.Results: The incidence of fetal distress in cases with cord around neck foetus in vaginal delivery was no significant difference compared with the control group(P<0.05),wheareas the incidence of fetal distress in foetus with cord around neck for 2 week was significantly higher than that in case with cord around neck for 1 week.The incidence of asphyxia and difficult labor in the two groups had no significant difference(P>0.05).Conclusions: The parturient with cord around neck feotus should be given trial vaginal delivery.Close monitoring in delivery and timely and effective treatment may reduce the incidence of cesarean section and improve the outcome of perinatal infants.
2006, 31(3): 269-271.
Abstract:
Objective: To discuss the clinic value of management of the premature infants.Methods: The clinical data of 98 premature infants were reviewed.Results: Ninty-eight cases have different degrees of complications,including hyperbilirubinemia,cold injury syndrome,pneumonia,apnea,hypoglycemia,hypocalcemia,intracranial hemorrhage,pulmonary hemorrhage,septicemia,purulent meningitis,and ventriculitis.Eighty-two cases have recovered from illness.Sixteen cases have died.Death rate is 16.33%.Death rate of fetal age less than 32 weeks was obviously higher than that of more than 32 weeks(P<0.01).Death rate of birth weight less than(1 500 g) was obviously higher than that of more than 1 500 g(P<0.05).The causes of death were mainly pulmonary hemorrhage,intracranial hemorrhage,pneumonia,septicemia and purulent meningitis.Conclusions: Distinguish early and treat correctly the complications of the premature infant is the main measure to reduce the death rate of the premature infant.
Objective: To discuss the clinic value of management of the premature infants.Methods: The clinical data of 98 premature infants were reviewed.Results: Ninty-eight cases have different degrees of complications,including hyperbilirubinemia,cold injury syndrome,pneumonia,apnea,hypoglycemia,hypocalcemia,intracranial hemorrhage,pulmonary hemorrhage,septicemia,purulent meningitis,and ventriculitis.Eighty-two cases have recovered from illness.Sixteen cases have died.Death rate is 16.33%.Death rate of fetal age less than 32 weeks was obviously higher than that of more than 32 weeks(P<0.01).Death rate of birth weight less than(1 500 g) was obviously higher than that of more than 1 500 g(P<0.05).The causes of death were mainly pulmonary hemorrhage,intracranial hemorrhage,pneumonia,septicemia and purulent meningitis.Conclusions: Distinguish early and treat correctly the complications of the premature infant is the main measure to reduce the death rate of the premature infant.
2006, 31(3): 271-272.
Abstract:
Objective: To observe the clinical features of the newborns with aspiration pneumonia and to investigate the effect of phentolamine in treatment of the disease.Methods: Fifty-three newborns with aspiration pneumonia were randomly divided into phentolamine group(32 cases) and routine group(21 cases).In addition to the routine therapy of antibiotic,oxygen and support treatment,the test group were added phentolamine 2-5 μg·kg-1·min-1 twice a day,two hours at a time and the progress of the disease was observed.Results: The total effective rates were 93.7% and 61.9% in the phentolamine group and the routine group respectively.The difference between the two groups was remarkable(P<0.05);the time of ecphysesis and the hospital stay in the phentolamine group were significantly less than that of the routine group(P<0.001).Conclusions: The application of Phentolamine based on the routine treatment can obviously improve the clinical symptoms and shorten the hospital stay.It is safe and effective.
Objective: To observe the clinical features of the newborns with aspiration pneumonia and to investigate the effect of phentolamine in treatment of the disease.Methods: Fifty-three newborns with aspiration pneumonia were randomly divided into phentolamine group(32 cases) and routine group(21 cases).In addition to the routine therapy of antibiotic,oxygen and support treatment,the test group were added phentolamine 2-5 μg·kg-1·min-1 twice a day,two hours at a time and the progress of the disease was observed.Results: The total effective rates were 93.7% and 61.9% in the phentolamine group and the routine group respectively.The difference between the two groups was remarkable(P<0.05);the time of ecphysesis and the hospital stay in the phentolamine group were significantly less than that of the routine group(P<0.001).Conclusions: The application of Phentolamine based on the routine treatment can obviously improve the clinical symptoms and shorten the hospital stay.It is safe and effective.
2006, 31(3): 273-274.
Abstract:
Objective: To evaluate video-assisted minithoracotomy surgery(VAMTS) in the treatment of thoracic injuries.Methods: VAMTS were performed on 13 patients.Among them,9 were removed of clotted hemothorax or decortication of lung,(2 cases) were treated with lung lacerated wound patching technique,1 case with diaphragm lacerated wound patching and the diaphragmatic hernia natrium technique and 2 cases were removed of the foreign matters in the lung(bullet).Results: All the patients recovered well.There was no operative mortality or postoperative complications.Conclusions: VAMTS is a safe and effective technique for choracic injuries with the advantages of little trauma and cost efficacy.
Objective: To evaluate video-assisted minithoracotomy surgery(VAMTS) in the treatment of thoracic injuries.Methods: VAMTS were performed on 13 patients.Among them,9 were removed of clotted hemothorax or decortication of lung,(2 cases) were treated with lung lacerated wound patching technique,1 case with diaphragm lacerated wound patching and the diaphragmatic hernia natrium technique and 2 cases were removed of the foreign matters in the lung(bullet).Results: All the patients recovered well.There was no operative mortality or postoperative complications.Conclusions: VAMTS is a safe and effective technique for choracic injuries with the advantages of little trauma and cost efficacy.
2006, 31(3): 290-292.
Abstract:
Objective: To study the efficacy of metal stent combined with arterial intubation chemotherapy for treatment of advanced esophageal carcinoma.Methods: The self-expanding Ni-Ti memorized alloy stent was placed though the mouth of 19 patients with advanced esophageal carcinoma under fluoroscopic guidance.When the physical condition of the patients recovered 1-2 weeks later,selective arterial intubation chemotherapy was given to the location of the carcinoma with Seldinger method.Results: Twenty-one stents were used in 19 cases.Significant efficacy was obtained in all the patients.Effective arterial intubation chemotherapy was obtained in 4 cases,striking improvement in 11 cases and no response in 3 cases.One was out of contact after the first treatment.The total efficacy rate was 78.9%,with no severe complications.Conclusions: The metal stent for advanced esophageal carcinoma has certain efficacy in near future and can improve the long-term efficacy and the life quality of the patients when combined with arterial intubation chemotherapy.
Objective: To study the efficacy of metal stent combined with arterial intubation chemotherapy for treatment of advanced esophageal carcinoma.Methods: The self-expanding Ni-Ti memorized alloy stent was placed though the mouth of 19 patients with advanced esophageal carcinoma under fluoroscopic guidance.When the physical condition of the patients recovered 1-2 weeks later,selective arterial intubation chemotherapy was given to the location of the carcinoma with Seldinger method.Results: Twenty-one stents were used in 19 cases.Significant efficacy was obtained in all the patients.Effective arterial intubation chemotherapy was obtained in 4 cases,striking improvement in 11 cases and no response in 3 cases.One was out of contact after the first treatment.The total efficacy rate was 78.9%,with no severe complications.Conclusions: The metal stent for advanced esophageal carcinoma has certain efficacy in near future and can improve the long-term efficacy and the life quality of the patients when combined with arterial intubation chemotherapy.
2006, 31(3): 295-296.
Abstract:
Objective: To analyze and compare the anti-bacterial effects of 21 antibiotics on the nitrobacteria isolated from 3 hospitals in Huaibei area in order to direct proper use of drugs in clinics.Methods: A total of 326 nitrobacteria were tested by Micro Scan WalkAWay-40,Vitek-32 and Kirby-Beuer for drug sensitivity.The susceptibility test was conducted according to NCCLS formulated in 1999.Results: Among 326 strains of enterobacteriaceae were isolated Escherichia coli(216),Klebsiella pneumoniae(37),Klebsiella rhinoschleromatis(18),Enterobacter agglomerans(19),Enterobacter aerogenes(18) and Enterobacter cloacae(18).Escherichia coli and Klebsiella pneumoniae resistance to imipenem were the lowest(1.4% and 2.8%),and then were cefoperazon/sulbactam,piperacillin/tazobactam,gentamicin,amikacin.Conclusions: Of the 21 antibiotics tested,Imipenem is the most sensitive one.Clinical treatment should be performed according to the results of the susceptibility test.
Objective: To analyze and compare the anti-bacterial effects of 21 antibiotics on the nitrobacteria isolated from 3 hospitals in Huaibei area in order to direct proper use of drugs in clinics.Methods: A total of 326 nitrobacteria were tested by Micro Scan WalkAWay-40,Vitek-32 and Kirby-Beuer for drug sensitivity.The susceptibility test was conducted according to NCCLS formulated in 1999.Results: Among 326 strains of enterobacteriaceae were isolated Escherichia coli(216),Klebsiella pneumoniae(37),Klebsiella rhinoschleromatis(18),Enterobacter agglomerans(19),Enterobacter aerogenes(18) and Enterobacter cloacae(18).Escherichia coli and Klebsiella pneumoniae resistance to imipenem were the lowest(1.4% and 2.8%),and then were cefoperazon/sulbactam,piperacillin/tazobactam,gentamicin,amikacin.Conclusions: Of the 21 antibiotics tested,Imipenem is the most sensitive one.Clinical treatment should be performed according to the results of the susceptibility test.
2006, 31(3): 297-298.
Abstract:
Objective: To investigate the relationship between the serum levels of high-sensitivity c-reactive protein(hs-CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α) and the pathogenesis of vascular complication in patients with diabetes mellitus.Methods: Serum hs-CRP was measured by particle immunoturbidimetric method on analysis instrument Hitachi 7170A.IL-6 and TNF-α were measured by double antibody sandwich ELISA.Results: The serum levels of hs-CRP,IL-6,and TNF-α of participants with vascular disease were significantly increased than that of participants without vascular disease and that in normal controls,and the level of hsCRP was positively correlated with the levels of IL-6,and TNF-α in type 2 diabetes,but not in normal controls.Conclusions: IL-6,and TNF-α,positively correlated with hs-CRP could play important roles in pathogenesis and progression of type 2 diabetes vascular complication.
Objective: To investigate the relationship between the serum levels of high-sensitivity c-reactive protein(hs-CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α) and the pathogenesis of vascular complication in patients with diabetes mellitus.Methods: Serum hs-CRP was measured by particle immunoturbidimetric method on analysis instrument Hitachi 7170A.IL-6 and TNF-α were measured by double antibody sandwich ELISA.Results: The serum levels of hs-CRP,IL-6,and TNF-α of participants with vascular disease were significantly increased than that of participants without vascular disease and that in normal controls,and the level of hsCRP was positively correlated with the levels of IL-6,and TNF-α in type 2 diabetes,but not in normal controls.Conclusions: IL-6,and TNF-α,positively correlated with hs-CRP could play important roles in pathogenesis and progression of type 2 diabetes vascular complication.
2006, 31(3): 304-306.
Abstract:
Objective: To investigate the mental health status of nursing university freshmen.Methods: Using the University Personality Inventory(UPI),the author made the research on 236 nursing university students.Results: The general mental health status of nursing university freshmen,which the intermediate were in the major,was below the level of undergraduate in the nation.63.56% nursing freshmen have psychological problems,52.97%-70.76% nurse presenced sensitivity and lack of self-confidence in cognize,more than half of the students presented tendency of neurasthenia or depression,and instability.Gender and discipline of students had no effect on the mental health.Conclusions: Being freshmen,derivable mostly from country,heavy tasks,weakness of mental education in school and the family were the influential factors which caused the low level of nursing students.By means of inquisition for freshmen,multifarious mental health education,training the correlative teachers and developing social practice,the school could reinforce the mental health of university students.
Objective: To investigate the mental health status of nursing university freshmen.Methods: Using the University Personality Inventory(UPI),the author made the research on 236 nursing university students.Results: The general mental health status of nursing university freshmen,which the intermediate were in the major,was below the level of undergraduate in the nation.63.56% nursing freshmen have psychological problems,52.97%-70.76% nurse presenced sensitivity and lack of self-confidence in cognize,more than half of the students presented tendency of neurasthenia or depression,and instability.Gender and discipline of students had no effect on the mental health.Conclusions: Being freshmen,derivable mostly from country,heavy tasks,weakness of mental education in school and the family were the influential factors which caused the low level of nursing students.By means of inquisition for freshmen,multifarious mental health education,training the correlative teachers and developing social practice,the school could reinforce the mental health of university students.
2006, 31(3): 309-310.
Abstract:
Objective: To observe the clinical characteristics of early chronic renal failure complicated by hypertension.Methods: Forty-one cases of early chronic renal failure accompanied by hypertension were observed and intervened by health education,mental nursing,diet and drugs.Results: Of the 41 cases,38 were complicated by hypertension(92.7%),including 12 cases of hypertension at the first stage(31.6%),10 cases at the second stage(26.3%) and 16 cases at the third stage(42.1%).By intervention,the blood pressure of the patients with early chronic renal failure decreased to some degree and the clinical symptoms were improved.Conclusions: Hypertension often occurs in patients with chronic renal failure at early stage.Early intervention may delay the progress of chronic renal failure.
Objective: To observe the clinical characteristics of early chronic renal failure complicated by hypertension.Methods: Forty-one cases of early chronic renal failure accompanied by hypertension were observed and intervened by health education,mental nursing,diet and drugs.Results: Of the 41 cases,38 were complicated by hypertension(92.7%),including 12 cases of hypertension at the first stage(31.6%),10 cases at the second stage(26.3%) and 16 cases at the third stage(42.1%).By intervention,the blood pressure of the patients with early chronic renal failure decreased to some degree and the clinical symptoms were improved.Conclusions: Hypertension often occurs in patients with chronic renal failure at early stage.Early intervention may delay the progress of chronic renal failure.
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