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2006 Vol. 31, No. 5
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2006, 31(5): 441-444.
Abstract:
Objective: To prepare anti-onchosphere rabbit sera and observe the in vivo expression of recombinant plasmid pcDNA3.1/TSO45-4B containing gene of stage-specific antigen TSO45-4B of Taenia solium onchosphere.Methods: The soluble antigen of onchosphere collected from hatched eggs was injected into rabbits,and the anti-onchosphere rabbit sera were collected and detected by Dot-Blot and ELISA.The recombinant plasmid pcDNA3.1/TSO45-4B was injected into Kunming mice's skeletal muscle and its expression was detected by immunohistochemistry assay.Results: The titer of polyclonal antibody to antigen of onchosphere in rabbit serum was(1:20 100).The specific masculine brown-yellow color reaction was seen in the muscle fibers of recombinant plasmid injected group.Conclusions: The successful expression of pcDNA3.1/TSO45-4B has provided the basis of in vivo expression for developing DNA vaccine for prevention of cysticercosis.
Objective: To prepare anti-onchosphere rabbit sera and observe the in vivo expression of recombinant plasmid pcDNA3.1/TSO45-4B containing gene of stage-specific antigen TSO45-4B of Taenia solium onchosphere.Methods: The soluble antigen of onchosphere collected from hatched eggs was injected into rabbits,and the anti-onchosphere rabbit sera were collected and detected by Dot-Blot and ELISA.The recombinant plasmid pcDNA3.1/TSO45-4B was injected into Kunming mice's skeletal muscle and its expression was detected by immunohistochemistry assay.Results: The titer of polyclonal antibody to antigen of onchosphere in rabbit serum was(1:20 100).The specific masculine brown-yellow color reaction was seen in the muscle fibers of recombinant plasmid injected group.Conclusions: The successful expression of pcDNA3.1/TSO45-4B has provided the basis of in vivo expression for developing DNA vaccine for prevention of cysticercosis.
2006, 31(5): 444-446.
Abstract:
Objective: To establish the ideal animal model for the study of Perthes disease.Methods: Twenty healthy young dogs were randomily divided into two groups.Dogs in one group were injected into muscle with methylprednisolone twice a week for 2 moths.Dogs in other groups were frozen by liquid nitrogen.Xray,MRI,ECT and histology examination were made periodically after experiment to evaluate osteonecrosis.Results: Necrosis of the femoral head in 4th and 8th week was confirmed in two experiment groups.The success rates were 40.0% in 4th week and 75.0% in 8th week with the methylprednisolone group,while in the liquid nitrogen group,the success rates were 90.0% in 4th week and 100.0% in 8th week.Conclusions: The model of Perthes disease could be built up by steroid-injection or liquid nitrogen in young dogs,but not all the dogs model can be built by steroid-injection.The animal model of Perthes disease with liquid nitrogen in young dogs can be established.
Objective: To establish the ideal animal model for the study of Perthes disease.Methods: Twenty healthy young dogs were randomily divided into two groups.Dogs in one group were injected into muscle with methylprednisolone twice a week for 2 moths.Dogs in other groups were frozen by liquid nitrogen.Xray,MRI,ECT and histology examination were made periodically after experiment to evaluate osteonecrosis.Results: Necrosis of the femoral head in 4th and 8th week was confirmed in two experiment groups.The success rates were 40.0% in 4th week and 75.0% in 8th week with the methylprednisolone group,while in the liquid nitrogen group,the success rates were 90.0% in 4th week and 100.0% in 8th week.Conclusions: The model of Perthes disease could be built up by steroid-injection or liquid nitrogen in young dogs,but not all the dogs model can be built by steroid-injection.The animal model of Perthes disease with liquid nitrogen in young dogs can be established.
2006, 31(5): 447-450.
Abstract:
Objective: To evaluate the clinicopathological significance of expressions of c-erbB-2,p53,proliferating cell nuclear antigen(PCNA),DNA topoisomerase Ⅱ(TopoⅡ),estrogen receptor(ER) and progesterone receptor(PR) in breast cancer.Methods: Eighty-nine cases of paraffin-embedded specimens of breast carcinoma were studied by immunohistochemical technique.Results: The positive rates of c-erbB-2,p53,TopoⅡand PCNA were 64.04%,29.21%,55.06%,and 59.55%,respectively.The expressions of c-erbB-2,PCNA were positively correlated with lymph node metastases(P<0.05),and there was the closely relationship between PCNA and invasive mammary carcinoma.But there was no relationship between TopoⅡ and 1ymph node metastases(P>0.05).Conclusions: The results suggested that c-erbB-2 and PCNA can be effective markers for estimating the prognosis of breast cancer.The expressions of TopoⅡ may be useful in predicting prognosis and leading chemotherapy.
Objective: To evaluate the clinicopathological significance of expressions of c-erbB-2,p53,proliferating cell nuclear antigen(PCNA),DNA topoisomerase Ⅱ(TopoⅡ),estrogen receptor(ER) and progesterone receptor(PR) in breast cancer.Methods: Eighty-nine cases of paraffin-embedded specimens of breast carcinoma were studied by immunohistochemical technique.Results: The positive rates of c-erbB-2,p53,TopoⅡand PCNA were 64.04%,29.21%,55.06%,and 59.55%,respectively.The expressions of c-erbB-2,PCNA were positively correlated with lymph node metastases(P<0.05),and there was the closely relationship between PCNA and invasive mammary carcinoma.But there was no relationship between TopoⅡ and 1ymph node metastases(P>0.05).Conclusions: The results suggested that c-erbB-2 and PCNA can be effective markers for estimating the prognosis of breast cancer.The expressions of TopoⅡ may be useful in predicting prognosis and leading chemotherapy.
2006, 31(5): 450-453.
Abstract:
Objective: To observe the inhibitory effect of sulindac on the growth of gastric cancer cells BGC-823 and its antineoplastic mechanisms.Methods: Human gastric cancer cells BGC-823 were cultured with various concentrations of sulindac for different time.The proliferation of BGC-823 cells was measured by MTT colorimetrie assay;and the cell cycle distribution was examined by flow cytometry.Cell apoptosis was determind by TUNEL.The expressions of ki-67,survivin and COX-2 in cells were detected by immunohistochemical staining.Results: Sulindac inhibited proliferation of BGC-823 cells,increased the proportion of cells in the G0/G1 phase,decreased the proportion of cells in the S phase,induced apoptosis of the BGC-823 cell line and decreased the expressions of ki-67,survivin and COX-2 in cells.All the effects were in a time-dependent and dose-dependent manner(P<0.05).Conclusions: Sulindac inhibits the growth of gastric cancer cells BGC-823 and the antitumor mechanism may be related to changing cell cycle distribution,inducing cell apoptosis and inhibiting the expression of COX-2,survivin and ki-67.
Objective: To observe the inhibitory effect of sulindac on the growth of gastric cancer cells BGC-823 and its antineoplastic mechanisms.Methods: Human gastric cancer cells BGC-823 were cultured with various concentrations of sulindac for different time.The proliferation of BGC-823 cells was measured by MTT colorimetrie assay;and the cell cycle distribution was examined by flow cytometry.Cell apoptosis was determind by TUNEL.The expressions of ki-67,survivin and COX-2 in cells were detected by immunohistochemical staining.Results: Sulindac inhibited proliferation of BGC-823 cells,increased the proportion of cells in the G0/G1 phase,decreased the proportion of cells in the S phase,induced apoptosis of the BGC-823 cell line and decreased the expressions of ki-67,survivin and COX-2 in cells.All the effects were in a time-dependent and dose-dependent manner(P<0.05).Conclusions: Sulindac inhibits the growth of gastric cancer cells BGC-823 and the antitumor mechanism may be related to changing cell cycle distribution,inducing cell apoptosis and inhibiting the expression of COX-2,survivin and ki-67.
2006, 31(5): 454-456.
Abstract:
Objective: To seek an advantageous anesthetic time for taxis of palate.Methods: One hundred and eighty patients with lateral complete cleft palate were randomly divided into 3 groups according to their ages:patients in group A were 1-2 years old,group B were 2-3 years old and group C were 4-7 years old.SpO2 during anesthesia and the anesthetic complications were observed.Results: SpO2 was significantly higher in group B and C than in group A(P<0.01).Complications such as laryngospasm,tracheospasm and glossocoma were significantly fewer in group B and C than in group A(P<0.05),while the differences between group B and group C were not significant(P>0.05).Conclusions: The advantageous anesthetic time for cleft palate taxis is during 2-3 years of age.
Objective: To seek an advantageous anesthetic time for taxis of palate.Methods: One hundred and eighty patients with lateral complete cleft palate were randomly divided into 3 groups according to their ages:patients in group A were 1-2 years old,group B were 2-3 years old and group C were 4-7 years old.SpO2 during anesthesia and the anesthetic complications were observed.Results: SpO2 was significantly higher in group B and C than in group A(P<0.01).Complications such as laryngospasm,tracheospasm and glossocoma were significantly fewer in group B and C than in group A(P<0.05),while the differences between group B and group C were not significant(P>0.05).Conclusions: The advantageous anesthetic time for cleft palate taxis is during 2-3 years of age.
2006, 31(5): 456-458.
Abstract:
Objective: To study the immunological characteristics of patients with hepatolenticular degeneration(HLD).Methods: The levels of serum immunoglobulins,complement and T-lymphocyte subsets,the indexes of the humoral immunity and the propotion of CD19+ in 21 patients with HLD and 19 normal controls were measured with immune turbidimetry and flow cytometry.Results: The levels of IgG and IgM in group HLD were increased compared with the controls,except for C4,which was depressed(P<0.05~P<0.01),together with the ratio of CD4+/CD8+,the propotion of CD3-CD16+CD56+、CD4+ increased(P<0.05~P<0.01).Conclusions: The study showed that there was disorder of the immunological state in patients with HLD,and suggests the manifestation of which is activation of the humoral immunity and depression of the cellular immunity.
Objective: To study the immunological characteristics of patients with hepatolenticular degeneration(HLD).Methods: The levels of serum immunoglobulins,complement and T-lymphocyte subsets,the indexes of the humoral immunity and the propotion of CD19+ in 21 patients with HLD and 19 normal controls were measured with immune turbidimetry and flow cytometry.Results: The levels of IgG and IgM in group HLD were increased compared with the controls,except for C4,which was depressed(P<0.05~P<0.01),together with the ratio of CD4+/CD8+,the propotion of CD3-CD16+CD56+、CD4+ increased(P<0.05~P<0.01).Conclusions: The study showed that there was disorder of the immunological state in patients with HLD,and suggests the manifestation of which is activation of the humoral immunity and depression of the cellular immunity.
2006, 31(5): 459-461.
Abstract:
Objective: To study the clinical significance of partial splenic embolization(PSE) in treatmeot of hypersplenism with liver cirrhosis.Methods: Twenty patients with liver cirrhosis and hypersplenism were treated with gelfoam(1 mm×1 mm×1 mm).The embolization degree ranged from 50% to 70%.Results: White blood cell(WBC) count increased immediately on the first day after PSE.WBC and Platelet(PLT) counts reached peak level on the third day and in the second week respectively after PSE,and then decreased gradually.WBC and PLT counts were normal or close to normal after one month.Neither severe side effects nor complications occurred.Conclusions: The embolization of the degree ranging from 50% to 70% may alleviate hypersplenism in patients with liver cirrhosis.
Objective: To study the clinical significance of partial splenic embolization(PSE) in treatmeot of hypersplenism with liver cirrhosis.Methods: Twenty patients with liver cirrhosis and hypersplenism were treated with gelfoam(1 mm×1 mm×1 mm).The embolization degree ranged from 50% to 70%.Results: White blood cell(WBC) count increased immediately on the first day after PSE.WBC and Platelet(PLT) counts reached peak level on the third day and in the second week respectively after PSE,and then decreased gradually.WBC and PLT counts were normal or close to normal after one month.Neither severe side effects nor complications occurred.Conclusions: The embolization of the degree ranging from 50% to 70% may alleviate hypersplenism in patients with liver cirrhosis.
2006, 31(5): 461-463.
Abstract:
Objective: To compare the effects of different dosages of fentanyl combined with propofol on plasma cardiac troponin-I(cTn-I) and management of fast track cardiac anesthesia(FTCA) in patients undergoing open heart surgery with cardiopulmonary bypass(CPB).Methods: Thirty patients,scheduled for undergoing atrioseptopexy or ventriseptopexy were randomly divided into three fentanyl dosage groups,ten patients each group.Fentanyl of 15 μg/kg was administered in group A,30 μg/kg in group B and 50 μg/kg in group C.Three groups were combined with intravenous infusion of propofol 2 to 4 mg·kg-1·h-1.The radial artery blood samples were collected to measure the plasma concentration of cTn-I before operation(T1),at the onset of aorta unclamping(T2),30 min after aorta unclamping(T3),4 h after weaning from CPB(T4) and 24 h after weaning from CPB(T5).ECG,HR,BP,CVP,SpO2,PETCO2,and body temperature(naso-pharyngeal and rectal) were continuously monitored during operation.Time of emergence from anesthesia and exlubation and the duration of stay in ICU were recorded.Results: The plasma concentration of cTn-I was increased significantly in three groups at T3,T4 and T5 compared with those at(T1)(P<0.01) and reached the peak level at T4 then was decreased at T5.cTn-I values in group B.Group C were lower than those in group A(P<0.01).There were no significant differences between group B and C(P>0.05).Time of emergence from anesthesia were significantly shorter in group A than in group B and C(P<0.05).Time of extubation were significantly shorter in group A and B than in group C(P<0.01 and P<0.05).The duration of stay in ICU showed a tendency of increase but there were no significant differences in three groups(P>0.05).Conclusions: Fentanyl 30 μg/kg combined with propofol 2 to 4 mg·kg-1·h-1 is suitable for protecting the myocardium from reperfusion injury and managemant of FTCA.
Objective: To compare the effects of different dosages of fentanyl combined with propofol on plasma cardiac troponin-I(cTn-I) and management of fast track cardiac anesthesia(FTCA) in patients undergoing open heart surgery with cardiopulmonary bypass(CPB).Methods: Thirty patients,scheduled for undergoing atrioseptopexy or ventriseptopexy were randomly divided into three fentanyl dosage groups,ten patients each group.Fentanyl of 15 μg/kg was administered in group A,30 μg/kg in group B and 50 μg/kg in group C.Three groups were combined with intravenous infusion of propofol 2 to 4 mg·kg-1·h-1.The radial artery blood samples were collected to measure the plasma concentration of cTn-I before operation(T1),at the onset of aorta unclamping(T2),30 min after aorta unclamping(T3),4 h after weaning from CPB(T4) and 24 h after weaning from CPB(T5).ECG,HR,BP,CVP,SpO2,PETCO2,and body temperature(naso-pharyngeal and rectal) were continuously monitored during operation.Time of emergence from anesthesia and exlubation and the duration of stay in ICU were recorded.Results: The plasma concentration of cTn-I was increased significantly in three groups at T3,T4 and T5 compared with those at(T1)(P<0.01) and reached the peak level at T4 then was decreased at T5.cTn-I values in group B.Group C were lower than those in group A(P<0.01).There were no significant differences between group B and C(P>0.05).Time of emergence from anesthesia were significantly shorter in group A than in group B and C(P<0.05).Time of extubation were significantly shorter in group A and B than in group C(P<0.01 and P<0.05).The duration of stay in ICU showed a tendency of increase but there were no significant differences in three groups(P>0.05).Conclusions: Fentanyl 30 μg/kg combined with propofol 2 to 4 mg·kg-1·h-1 is suitable for protecting the myocardium from reperfusion injury and managemant of FTCA.
2006, 31(5): 464-465.
Abstract:
Objective: To explore the diagnosis and treatment of hemophagocytic syndrome(HPS).Methods: The clinical manifestations,laboratory findings,diagnosis and treatment of 8 cases of HPS were analyzed and reviewed with current literature.Results: Prolonged fever with unknown origin and hepatosplenomegaly were present in all the 8 cases(splenomegaly in 5 cases).Cytopenia with bilineage in the peripheral blood was found in 3 cases and pancytopenia in 5 cases.Hemophagocytosis in bone marrow was easily observed.The only patient infected by cytomegalovirus recovered rapidly after effective treatment.Conclusions: HPS is a rare disease with complex clinical presentation.Bone marrow aspiration should be performed in time or repeatedly to increase the diagnosis rate,which is of great value to further treatment.
Objective: To explore the diagnosis and treatment of hemophagocytic syndrome(HPS).Methods: The clinical manifestations,laboratory findings,diagnosis and treatment of 8 cases of HPS were analyzed and reviewed with current literature.Results: Prolonged fever with unknown origin and hepatosplenomegaly were present in all the 8 cases(splenomegaly in 5 cases).Cytopenia with bilineage in the peripheral blood was found in 3 cases and pancytopenia in 5 cases.Hemophagocytosis in bone marrow was easily observed.The only patient infected by cytomegalovirus recovered rapidly after effective treatment.Conclusions: HPS is a rare disease with complex clinical presentation.Bone marrow aspiration should be performed in time or repeatedly to increase the diagnosis rate,which is of great value to further treatment.
2006, 31(5): 466-467.
Abstract:
Objective: To study the changes of the level of urinary acetylglucosaminidase(N-acetyl-β-D-glucosaminidase,NAG) in patients with chronic renal failure(CRF) and its clinical significance in the development of CRF.Methods: Eight-seven patients were divided into 2 groups according to the stability of the disease and the level of blood pressure;and thirty healthy persons acted as controls.Their NAG activity was measured by colorimetry test and compared.Results: The urinary NAG level in CRF patients was significantly higher than that of the controls(P<0.01).The urinary NAG level in patients with unstable CRF was significantly higher than that in patients with stable CRF(P<0.01),and the urinary NAG level of CRF patients with hypertension was higher than that of patients without hypertension(P<0.01).Conclusions: The instability of CRF and hypertension may be pivotal factors that aggravate tubulointerstital injury.
Objective: To study the changes of the level of urinary acetylglucosaminidase(N-acetyl-β-D-glucosaminidase,NAG) in patients with chronic renal failure(CRF) and its clinical significance in the development of CRF.Methods: Eight-seven patients were divided into 2 groups according to the stability of the disease and the level of blood pressure;and thirty healthy persons acted as controls.Their NAG activity was measured by colorimetry test and compared.Results: The urinary NAG level in CRF patients was significantly higher than that of the controls(P<0.01).The urinary NAG level in patients with unstable CRF was significantly higher than that in patients with stable CRF(P<0.01),and the urinary NAG level of CRF patients with hypertension was higher than that of patients without hypertension(P<0.01).Conclusions: The instability of CRF and hypertension may be pivotal factors that aggravate tubulointerstital injury.
2006, 31(5): 468-469.
Abstract:
Objective: To observe the clinical response and side effect of docetaxel combined with cisplatin in treatment of advanced breast cancer.Methods: All the patients were administered docetaxel 70 mg/m2 iv drip d1,and dexamethasone 7.5 mg bid d1-3 twenty-four hours before chemotherapy.Cisplatin 25-30 mg/m2 was given iv drip d1-3,every 3 weeks as a circle.Results: Five cases presented complete remission(CR),and 29 cases partial remission(PR).The total response rate(PR+CR) was 72.3%.The main toxicity was neutropenia,nausea and vomiting.Conclusions: The regimen of docetaxel combined with cisplatin has significant antitumor activity for patients with advanced breast cancer.
Objective: To observe the clinical response and side effect of docetaxel combined with cisplatin in treatment of advanced breast cancer.Methods: All the patients were administered docetaxel 70 mg/m2 iv drip d1,and dexamethasone 7.5 mg bid d1-3 twenty-four hours before chemotherapy.Cisplatin 25-30 mg/m2 was given iv drip d1-3,every 3 weeks as a circle.Results: Five cases presented complete remission(CR),and 29 cases partial remission(PR).The total response rate(PR+CR) was 72.3%.The main toxicity was neutropenia,nausea and vomiting.Conclusions: The regimen of docetaxel combined with cisplatin has significant antitumor activity for patients with advanced breast cancer.
2006, 31(5): 470-472.
Abstract:
Objective: To evaluate the therapeutic effect and toxicity of COMxEP regimens in treatment of non-Hodgkin's lymphoma(NHL).Methods: Thirty-seven patients with intermediate-high grade NHL were treated with a combination chemotherapy of COMxEP.COMxEP regimen consisted of VP16,60-70 mg/m2(iv gtt,Day1-5);MxT,6-8 mg/m2(iv gtt,Day1);VCR,1.4 mg/m2(iv,Day1,8);CTX,600-800 mg(iv,Day1,8);Pred,40-60 mg/day(po,Day1-5).One course lasted for 4 weeks and each patient received(4.8) courses on the average.Results: In the thirty seven patients receiving COMxEP regimen,complete remission(CR) rate was(67.6%),partial remission(PR) rate 21.6%,SD rate 14.3% and PD was no,and the overall response rate was 89.2%(33/37),with a median duration of 26 months.In the patients with refractory disease,CR rate was 53.8%,PR rate 30.8%,SD rate 15.4% and PD was no,and the overall response rate was 84.6%.The main side effects were bone marrow depression,enteron reaction and alopecie.Conclusions: COMxEP regimen is effective for patients with newly diagnosed or refractory disease,and the toxicity is tolerable.This regimen can be considered as a routine or salvage therapy for intermediate-high grade NHL.
Objective: To evaluate the therapeutic effect and toxicity of COMxEP regimens in treatment of non-Hodgkin's lymphoma(NHL).Methods: Thirty-seven patients with intermediate-high grade NHL were treated with a combination chemotherapy of COMxEP.COMxEP regimen consisted of VP16,60-70 mg/m2(iv gtt,Day1-5);MxT,6-8 mg/m2(iv gtt,Day1);VCR,1.4 mg/m2(iv,Day1,8);CTX,600-800 mg(iv,Day1,8);Pred,40-60 mg/day(po,Day1-5).One course lasted for 4 weeks and each patient received(4.8) courses on the average.Results: In the thirty seven patients receiving COMxEP regimen,complete remission(CR) rate was(67.6%),partial remission(PR) rate 21.6%,SD rate 14.3% and PD was no,and the overall response rate was 89.2%(33/37),with a median duration of 26 months.In the patients with refractory disease,CR rate was 53.8%,PR rate 30.8%,SD rate 15.4% and PD was no,and the overall response rate was 84.6%.The main side effects were bone marrow depression,enteron reaction and alopecie.Conclusions: COMxEP regimen is effective for patients with newly diagnosed or refractory disease,and the toxicity is tolerable.This regimen can be considered as a routine or salvage therapy for intermediate-high grade NHL.
2006, 31(5): 472-474.
Abstract:
Objective: To investigate the effect of early postoperative enteral nutrition(EN) on the function of intestinal mucosa after total gastrectomy.Methods: Twenty-nine patients undergoing total gastrectomy for gastric cancer were randomly divided into EN group and parenteral nutrition(PN) group.Both groups received the same amount of nitrogen and calorie from 24 hours to the eighth day after operation,and the serum level of peripheral immuinoglobulins and serum diamine oxidase(DAO) were measured.Results: DAO activity decreased in both groups.EN group recovered within 1 week but PN group did not recover until the 14th day.The level of IgA in EN group was significant higher than that in PN group as well as that of EN group before operation(P<0.01).Conclusions: Early EN can alleviate the immune suppression and preserve the integrity of intestinal mucosa barrier after total gastrectomy.
Objective: To investigate the effect of early postoperative enteral nutrition(EN) on the function of intestinal mucosa after total gastrectomy.Methods: Twenty-nine patients undergoing total gastrectomy for gastric cancer were randomly divided into EN group and parenteral nutrition(PN) group.Both groups received the same amount of nitrogen and calorie from 24 hours to the eighth day after operation,and the serum level of peripheral immuinoglobulins and serum diamine oxidase(DAO) were measured.Results: DAO activity decreased in both groups.EN group recovered within 1 week but PN group did not recover until the 14th day.The level of IgA in EN group was significant higher than that in PN group as well as that of EN group before operation(P<0.01).Conclusions: Early EN can alleviate the immune suppression and preserve the integrity of intestinal mucosa barrier after total gastrectomy.
2006, 31(5): 475-476.
Abstract:
Objective: To understand the gap between the current practice of medications on patients with acute myocardial infarction(AMI) in hospital and guideline application.Methods: Ninety-two patients with AMI from Tongcheng People's Hospital received different therapies in Department of Cardiology from December 2002 to November 2005.The application of aspirin,low molecular heparin(LWMH),Nitrates,β-blocker,angiotensin converting enzyme inhibitors(ACEI),reperfusion,percutaneous corongary intervention(PCI) and cholesterol lowering agents were analyzed on different AMI patients.Results: Percentage of medications in AMI patients in different periods was:aspirin 90.32% to 93.10%,LWMH 70.97% to 82.76%,Nitrates 78.13% to 80.65%,β-blocker 62.50% to 65.52%,ACEI 46.88% to 58.62%,cholesterol lowering agents 6.25% to 68.79%,Fibrinolytics 31.25% to 41.38%,PCI3.13% to 17.24%.Conclusions: Application of medications proved effective by evidence-based medicine in clinical practice is mostly better in the class A gradeⅡ hospitals,and partly equal to the class A grade Ⅲ hospitals.But the application in China could be further improved.The gap of PCI application is quite big and it's hard to improve fast.Fibrinolytics is still the main method of reperfusion in the early period.
Objective: To understand the gap between the current practice of medications on patients with acute myocardial infarction(AMI) in hospital and guideline application.Methods: Ninety-two patients with AMI from Tongcheng People's Hospital received different therapies in Department of Cardiology from December 2002 to November 2005.The application of aspirin,low molecular heparin(LWMH),Nitrates,β-blocker,angiotensin converting enzyme inhibitors(ACEI),reperfusion,percutaneous corongary intervention(PCI) and cholesterol lowering agents were analyzed on different AMI patients.Results: Percentage of medications in AMI patients in different periods was:aspirin 90.32% to 93.10%,LWMH 70.97% to 82.76%,Nitrates 78.13% to 80.65%,β-blocker 62.50% to 65.52%,ACEI 46.88% to 58.62%,cholesterol lowering agents 6.25% to 68.79%,Fibrinolytics 31.25% to 41.38%,PCI3.13% to 17.24%.Conclusions: Application of medications proved effective by evidence-based medicine in clinical practice is mostly better in the class A gradeⅡ hospitals,and partly equal to the class A grade Ⅲ hospitals.But the application in China could be further improved.The gap of PCI application is quite big and it's hard to improve fast.Fibrinolytics is still the main method of reperfusion in the early period.
2006, 31(5): 477-478.
Abstract:
Objective: To analyze the risk factors for recurrent peptic ulcers bleeding.Methods: The clinical data such as sex,age,size of ulcer,volume of blooding,H.pylori infection,stomachache,presence of concomitant diseases,history of treatment(NSAID),nutritional status,eating habit,labor intensity,sleep,etc were collected in 84 peptic ulcer patients combined with hemorrhage.Results: The occurrence of hemorrhage was observed in 70.73% of the patients,gastric ulcer in 26.83%,patients with age under 50 years in 78.05%,initial treatment in 87.80%,H.pylori infection in 86.59% and wakefulness in 78.05%.The male accounted for 76.83% and the female 23.17%.The other important risk factors were eating habit,exertion and undernutrition.Conclusions: We suggest that lack of treatment,H.pylori infection,age<50,wakefulness,eating habit,labor intensity,undernutrition are the risk factors for recurrent peptic ulcer hemorrhage.
Objective: To analyze the risk factors for recurrent peptic ulcers bleeding.Methods: The clinical data such as sex,age,size of ulcer,volume of blooding,H.pylori infection,stomachache,presence of concomitant diseases,history of treatment(NSAID),nutritional status,eating habit,labor intensity,sleep,etc were collected in 84 peptic ulcer patients combined with hemorrhage.Results: The occurrence of hemorrhage was observed in 70.73% of the patients,gastric ulcer in 26.83%,patients with age under 50 years in 78.05%,initial treatment in 87.80%,H.pylori infection in 86.59% and wakefulness in 78.05%.The male accounted for 76.83% and the female 23.17%.The other important risk factors were eating habit,exertion and undernutrition.Conclusions: We suggest that lack of treatment,H.pylori infection,age<50,wakefulness,eating habit,labor intensity,undernutrition are the risk factors for recurrent peptic ulcer hemorrhage.
2006, 31(5): 479-481.
Abstract:
Objective: To explore the diagnosis and treatment of malignant gastric stromal tumors.Methods: The clinical data of 20 cases of malignant gastric stromal tumor having received surgical management were reviewed.Fourteen cases underwent radical subtotal or total gastrectomy,5 local resections,1 parative total gastrectomy and 5 combined resection of other viscera.Results: Most of the patients with malignant gastric stromal tumors were adults.The patients ≥ 50 years of age accounted for 70%(14/20).Gastrointestinal hemorrhage,anemia and pain were the main clinical manifestations.There were no perioperative death and main postoperative complication,all cases healed.Conclusions: Endoscopy and imaging examination are the main methods to detect malignant gastric stromal tumors.Preoperation diagnosis is still difficult.Surgical resection is the main method for pathologic diagnosis and treatment.Sometimes combined resection of other viscera is necessary to ensure the tumor completely resected,which is of great value to prevent recurrence of the tumor.
Objective: To explore the diagnosis and treatment of malignant gastric stromal tumors.Methods: The clinical data of 20 cases of malignant gastric stromal tumor having received surgical management were reviewed.Fourteen cases underwent radical subtotal or total gastrectomy,5 local resections,1 parative total gastrectomy and 5 combined resection of other viscera.Results: Most of the patients with malignant gastric stromal tumors were adults.The patients ≥ 50 years of age accounted for 70%(14/20).Gastrointestinal hemorrhage,anemia and pain were the main clinical manifestations.There were no perioperative death and main postoperative complication,all cases healed.Conclusions: Endoscopy and imaging examination are the main methods to detect malignant gastric stromal tumors.Preoperation diagnosis is still difficult.Surgical resection is the main method for pathologic diagnosis and treatment.Sometimes combined resection of other viscera is necessary to ensure the tumor completely resected,which is of great value to prevent recurrence of the tumor.
2006, 31(5): 481-482.
Abstract:
Objective: To summarize the surgical treatment for ventricular septal defect(VSD) combined with pulmonary hypertension(PH) in children.Methods: Fifteen patients with ventricular septal defect combined with pulmonary hypertension diagnosed by color Doppler flow imaging(CDFI) were included in this group and underwent the Dacron patch repair under hypothermal cardiopulmonary by pass.Results: Death occurred in no patients after the operation.The low cardiac output occurred in two patients and the hydrothorax in one patient.The residual shunt of 2 mm was found in the CDFI in one patient when discharged from the hospital.The clinical symptoms disappeared and the state of the nutrition and the development for the patients was significantly improved in the follow-up 12 months.Conclusions: Most children with ventricular septal defect combined with pulmonary hypertension can be cured with early diagnosis and treatment.The operation is safe.After the operation the patient can recover completely.
Objective: To summarize the surgical treatment for ventricular septal defect(VSD) combined with pulmonary hypertension(PH) in children.Methods: Fifteen patients with ventricular septal defect combined with pulmonary hypertension diagnosed by color Doppler flow imaging(CDFI) were included in this group and underwent the Dacron patch repair under hypothermal cardiopulmonary by pass.Results: Death occurred in no patients after the operation.The low cardiac output occurred in two patients and the hydrothorax in one patient.The residual shunt of 2 mm was found in the CDFI in one patient when discharged from the hospital.The clinical symptoms disappeared and the state of the nutrition and the development for the patients was significantly improved in the follow-up 12 months.Conclusions: Most children with ventricular septal defect combined with pulmonary hypertension can be cured with early diagnosis and treatment.The operation is safe.After the operation the patient can recover completely.
2006, 31(5): 483-485.
Abstract:
Objective: To evaluate the clinical effects of ureteroscopic pneumatic lithotripsy for the treatment of ureteral steinstrasse.Methods: Eighteen patients with ureteral steinstrasse were treated with pneumatic lithotripsy and the calculi were removed under ureteroscope.Results: All the patients were treated successfully with the cure rate of 100%.The complication rate in the operation was 11.1%,including 2 patients with ureteral submucosal false passage.Conclusions: Treatment of ureteral steinstrasse with ureteroscope and pneumatic lithotripsy is minimally invasive and effective.
Objective: To evaluate the clinical effects of ureteroscopic pneumatic lithotripsy for the treatment of ureteral steinstrasse.Methods: Eighteen patients with ureteral steinstrasse were treated with pneumatic lithotripsy and the calculi were removed under ureteroscope.Results: All the patients were treated successfully with the cure rate of 100%.The complication rate in the operation was 11.1%,including 2 patients with ureteral submucosal false passage.Conclusions: Treatment of ureteral steinstrasse with ureteroscope and pneumatic lithotripsy is minimally invasive and effective.
2006, 31(5): 485-486.
Abstract:
Objective: To study the correlation between atrial fibrillation(AF) in acute myocardial infarction(AMI) and coronary lesions.Methods: Eighteen patients who developed AF after the onset of AMI were classified into AF group and 20 patients who did not develop AF after the onset of AMI were classified into non-AF group.According to the onset time of AF,AF group was divided into two subgroups:subgroup A(n=8,AF developed within 24 hours after the onset of AMI)and subgroup B(n=10,AF developed>24 hours after the onset of AMI).The angiographic outcome was compared between the two groups and the two subgroups.Results: The incidence of multivessel disease was significantly higher in AF group than in non-AF group(P<0.05).Occlusion of the right proximal coronary artery was responsible for AMI in subgroup A,while occlusion of the left anterior descending branch was responsible for AMI in subgroup B(P<0.05).Conclusions: The patients who developed AF in the course of MI demonstrates severer coronary lesion.The onset time of AF in AMI is related to the location of the infarct-related coronary lesions.
Objective: To study the correlation between atrial fibrillation(AF) in acute myocardial infarction(AMI) and coronary lesions.Methods: Eighteen patients who developed AF after the onset of AMI were classified into AF group and 20 patients who did not develop AF after the onset of AMI were classified into non-AF group.According to the onset time of AF,AF group was divided into two subgroups:subgroup A(n=8,AF developed within 24 hours after the onset of AMI)and subgroup B(n=10,AF developed>24 hours after the onset of AMI).The angiographic outcome was compared between the two groups and the two subgroups.Results: The incidence of multivessel disease was significantly higher in AF group than in non-AF group(P<0.05).Occlusion of the right proximal coronary artery was responsible for AMI in subgroup A,while occlusion of the left anterior descending branch was responsible for AMI in subgroup B(P<0.05).Conclusions: The patients who developed AF in the course of MI demonstrates severer coronary lesion.The onset time of AF in AMI is related to the location of the infarct-related coronary lesions.
2006, 31(5): 487-489.
Abstract:
Objective: To introduce the technique of minimally invasive percutaneous plate osteosynthesis(MIPPO) in treatment of tibial fractures and to observe the outcome.Methods: Twelve cases of tibial fractures were treated with MIPPO and no external fixation was used.Active and passive motion of ankle joint without weight-bearing was allowed two days after operation.Results: All the patients were followed up for 6 to 18 months.No infection,delayed union,nonunion or malunion were found.The mean time of bone union was 14 weeks.According to Johner-Wruhs score standard,the results were excellent in 8 cases,good in 3 cases and fair in 1 case.The excellent and good rates were 91.6%.Conclusions: MIPPO is a safe and effective technique for treatment of tibia fractures.
Objective: To introduce the technique of minimally invasive percutaneous plate osteosynthesis(MIPPO) in treatment of tibial fractures and to observe the outcome.Methods: Twelve cases of tibial fractures were treated with MIPPO and no external fixation was used.Active and passive motion of ankle joint without weight-bearing was allowed two days after operation.Results: All the patients were followed up for 6 to 18 months.No infection,delayed union,nonunion or malunion were found.The mean time of bone union was 14 weeks.According to Johner-Wruhs score standard,the results were excellent in 8 cases,good in 3 cases and fair in 1 case.The excellent and good rates were 91.6%.Conclusions: MIPPO is a safe and effective technique for treatment of tibia fractures.
2006, 31(5): 489-490.
Abstract:
Objective: To observe the change of heart rate variability(HRV) in patients with coronary artery disease(CAD) before and after percutaneous coronary intervention(PCI) and to evaluate the prognosis of CAD patients receiving PCI.Methods: Thirty-two PCI patients confirmed by coronary arteriography and suitable to PCI were divided into angina pectoris group(20 cases) and myocardial infarction group(12 cases).The time-domain(SDNN,SDANN,rMSSD,PNN50) and frequency-domain(LF,HF) of HRV were analyzed before PCI and one month,six months after PCI.Results: There was a significant improvement of SDNN,SDANN and HF(P<0.01) one month after PCI,and more improvement was observed in other indices(P<0.01) except rMSSD(P>0.05)six months after PCI.Conclusions: HRV in patients with CAD can be improved significantly after PCI,and it may be related to the improvement of myocardial circulation,and the correction of the dysfunction of sinus node and myocardium due to the unbalance of neuro-humoral factors.
Objective: To observe the change of heart rate variability(HRV) in patients with coronary artery disease(CAD) before and after percutaneous coronary intervention(PCI) and to evaluate the prognosis of CAD patients receiving PCI.Methods: Thirty-two PCI patients confirmed by coronary arteriography and suitable to PCI were divided into angina pectoris group(20 cases) and myocardial infarction group(12 cases).The time-domain(SDNN,SDANN,rMSSD,PNN50) and frequency-domain(LF,HF) of HRV were analyzed before PCI and one month,six months after PCI.Results: There was a significant improvement of SDNN,SDANN and HF(P<0.01) one month after PCI,and more improvement was observed in other indices(P<0.01) except rMSSD(P>0.05)six months after PCI.Conclusions: HRV in patients with CAD can be improved significantly after PCI,and it may be related to the improvement of myocardial circulation,and the correction of the dysfunction of sinus node and myocardium due to the unbalance of neuro-humoral factors.
2006, 31(5): 491-493.
Abstract:
Objective: To investigate the etiology and the treatment of postpartum hemorrhage.Methods: Strengthening uterine contraction,keeping effective circulation and removing etiological factor were used to treat 367 women with postpartum hemorrhage in our hospital.Results: The primary reason of postpartum hemorrhage was the weakness of uterine contraction in 209 cases.The placenta factor and laceration of birth canal was the second cause.The risk factors were the caesarean delivery,the large infant,the extension of labor cause,the residue of placenta or embryolema and all kinds of pregnancy complications.Conclusions: To prevent postpartum hemorrhage is one of the important measures to improve the quality of obstetrics and reduce the mortality of pregnant and lying-in women.
Objective: To investigate the etiology and the treatment of postpartum hemorrhage.Methods: Strengthening uterine contraction,keeping effective circulation and removing etiological factor were used to treat 367 women with postpartum hemorrhage in our hospital.Results: The primary reason of postpartum hemorrhage was the weakness of uterine contraction in 209 cases.The placenta factor and laceration of birth canal was the second cause.The risk factors were the caesarean delivery,the large infant,the extension of labor cause,the residue of placenta or embryolema and all kinds of pregnancy complications.Conclusions: To prevent postpartum hemorrhage is one of the important measures to improve the quality of obstetrics and reduce the mortality of pregnant and lying-in women.
2006, 31(5): 493-494.
Abstract:
Objective: To evaluate the clinical manifestation of diabetes mellitus in children and to improve the clinical diagnosis and management in children with type 1 diabetes mellitus(T1-DM).Methods: The clinical data of 32 children diagnosised as T1-DM between 1999 and 2006 in Anhui Provincial Children's Hospital were retrospectively analyzed,and the treatment outcomes of diabetes ketoacidosis(DKA) in 10 children were observed.Results: The preliminary symptoms of T1-DM were DKA in 10 patients,respiratory infection in 4 patients,vomiting in 3 patients and stomachache in one patient.The low-dosage insulin was effective to children with DKA.Conclusions: The onset of the T1-DM in children is usually latent with atypical clinical manifestations.The DKA,the respiratory and digestive diseases are the preliminary symptoms.Therefore it can be easily misdiagnosed and treated in wrong way.The insulin is effective to it.
Objective: To evaluate the clinical manifestation of diabetes mellitus in children and to improve the clinical diagnosis and management in children with type 1 diabetes mellitus(T1-DM).Methods: The clinical data of 32 children diagnosised as T1-DM between 1999 and 2006 in Anhui Provincial Children's Hospital were retrospectively analyzed,and the treatment outcomes of diabetes ketoacidosis(DKA) in 10 children were observed.Results: The preliminary symptoms of T1-DM were DKA in 10 patients,respiratory infection in 4 patients,vomiting in 3 patients and stomachache in one patient.The low-dosage insulin was effective to children with DKA.Conclusions: The onset of the T1-DM in children is usually latent with atypical clinical manifestations.The DKA,the respiratory and digestive diseases are the preliminary symptoms.Therefore it can be easily misdiagnosed and treated in wrong way.The insulin is effective to it.
2006, 31(5): 495-496.
Abstract:
Objective: To evaluate the effect of radiotherapy on gliomas and the prognostic factor.Methods: Seventy-four patients with gliomas were treated by radiotherapy.Among them,39 cases were in low grade(Ⅰ+Ⅱ grade),31 cases in high grade(Ⅲ+Ⅳ grade) and 4 cases had the ependyma tumor.All the patients received radiotherapy in 3-4 weeks after operation.Thirty-one cases were performed total resection,38 cases bulk-resection and 5 cases partial resection.Among them,16 cases of high grade received chemotherapy after radiotherapy.Results: The patients under the age of 45,the total resection group and the patients of lower-ranking in pathology had a higher survival rate(1,3 and 5 years)(P<0.05 to P<0.01).Conclusions: Radiotherapy is effective for patients with gliomas.Age,pathological grading,operation mode are the important prognosis factors.
Objective: To evaluate the effect of radiotherapy on gliomas and the prognostic factor.Methods: Seventy-four patients with gliomas were treated by radiotherapy.Among them,39 cases were in low grade(Ⅰ+Ⅱ grade),31 cases in high grade(Ⅲ+Ⅳ grade) and 4 cases had the ependyma tumor.All the patients received radiotherapy in 3-4 weeks after operation.Thirty-one cases were performed total resection,38 cases bulk-resection and 5 cases partial resection.Among them,16 cases of high grade received chemotherapy after radiotherapy.Results: The patients under the age of 45,the total resection group and the patients of lower-ranking in pathology had a higher survival rate(1,3 and 5 years)(P<0.05 to P<0.01).Conclusions: Radiotherapy is effective for patients with gliomas.Age,pathological grading,operation mode are the important prognosis factors.
2006, 31(5): 497-498.
Abstract:
Objective: To study the curative effect and side effect of thalidomide combined with chemotherapy in treatment of multiple myeloma.Methods: The treatment group(21 cases) was administered thalidomide at a dose of 200 mg/d and chemotherapy.The dosage increased to 300 mg/d after 4 weeks when the effect was not satisfactory,which was maintained for half a year.The chemotherapy regimens were MP,VAD and VMCP.The control group(24 cases) was given chemotherapy only.The chemotherapy regimens and doses were the same with the treatment group.Results: In the treatment group,3 cases had partial relief(61.90%),4 cases improved(19.05%) and 4 cases had no response(19.05%).The total efficacy rate was 80.95%,which was significantly higher than that of the control group(with a total efficacy rate of 54.17%)(P<0.01).The improvement rate of most indexes in the treatment group was higher than that in the control group(P<0.05-P<0.005).The side effects included constipation,lethargy,dermatitis and edema in the two low limbs,which were all tolerable.Conclusions: Thalidomide combined with chemotherapy in treatment of multiple myeloma has the merits of fewer side effects,good tolerance,convenient administration and significant curative effect.It is worth popularizing.
Objective: To study the curative effect and side effect of thalidomide combined with chemotherapy in treatment of multiple myeloma.Methods: The treatment group(21 cases) was administered thalidomide at a dose of 200 mg/d and chemotherapy.The dosage increased to 300 mg/d after 4 weeks when the effect was not satisfactory,which was maintained for half a year.The chemotherapy regimens were MP,VAD and VMCP.The control group(24 cases) was given chemotherapy only.The chemotherapy regimens and doses were the same with the treatment group.Results: In the treatment group,3 cases had partial relief(61.90%),4 cases improved(19.05%) and 4 cases had no response(19.05%).The total efficacy rate was 80.95%,which was significantly higher than that of the control group(with a total efficacy rate of 54.17%)(P<0.01).The improvement rate of most indexes in the treatment group was higher than that in the control group(P<0.05-P<0.005).The side effects included constipation,lethargy,dermatitis and edema in the two low limbs,which were all tolerable.Conclusions: Thalidomide combined with chemotherapy in treatment of multiple myeloma has the merits of fewer side effects,good tolerance,convenient administration and significant curative effect.It is worth popularizing.
2006, 31(5): 499-500.
Abstract:
Objective: To evaluate the efficacy and safety of doxifluridine in treatment of old and weak patients with advanced gastric and colorectal cancer.Methods: Twenty cases of advanced gastric and colorectal cancer were given doxifluridine 200 mg four times daily for 14 days,followed by a week rest.The regime was repeated for 2-3 cycles.Results: Among the patients,CR was found in 0 cases,PR in 6 cases,SD in 9 cases and PD in 5 cases.the overall response rate was 30%.There were few adverse events.WHO grade Ⅰ/Ⅱ leukopenia occurred in 5 cases(25%),grade I thrombocytopenia occurred in 2 cases(10%),gradeⅠ/Ⅱ nausea and vomiting in 3 cases(15%),and the damage to the liver and kidney and heart function was not obvious.Conclusions: Doxifluridine has certain effect on patients with advanced gastric and colorectal cancer,with slight toxin or side effects.
Objective: To evaluate the efficacy and safety of doxifluridine in treatment of old and weak patients with advanced gastric and colorectal cancer.Methods: Twenty cases of advanced gastric and colorectal cancer were given doxifluridine 200 mg four times daily for 14 days,followed by a week rest.The regime was repeated for 2-3 cycles.Results: Among the patients,CR was found in 0 cases,PR in 6 cases,SD in 9 cases and PD in 5 cases.the overall response rate was 30%.There were few adverse events.WHO grade Ⅰ/Ⅱ leukopenia occurred in 5 cases(25%),grade I thrombocytopenia occurred in 2 cases(10%),gradeⅠ/Ⅱ nausea and vomiting in 3 cases(15%),and the damage to the liver and kidney and heart function was not obvious.Conclusions: Doxifluridine has certain effect on patients with advanced gastric and colorectal cancer,with slight toxin or side effects.
2006, 31(5): 501-503.
Abstract:
Objective: To investigate the clinical characteristics,treatment of ocular trauma in children.Methods: Retrospective clinical analysis was done in 376 patients(392 eyes) with ocular trauma in children who were treated by our department in recent decade.Results: The first reason that caused blind in ocular trauma in children was perforating injury followed by blunt trauma.Without treatment,48.98%(192 eyes) were blind.After treatment,the blind was reduced to 32.91%(129eyes).The visual acuities of 146 eyes(37.24%) recovered over 0.3.Conclusions: The ocular trauma in children injured visual function seriously.Good effect can be obtained by proper treatment.
Objective: To investigate the clinical characteristics,treatment of ocular trauma in children.Methods: Retrospective clinical analysis was done in 376 patients(392 eyes) with ocular trauma in children who were treated by our department in recent decade.Results: The first reason that caused blind in ocular trauma in children was perforating injury followed by blunt trauma.Without treatment,48.98%(192 eyes) were blind.After treatment,the blind was reduced to 32.91%(129eyes).The visual acuities of 146 eyes(37.24%) recovered over 0.3.Conclusions: The ocular trauma in children injured visual function seriously.Good effect can be obtained by proper treatment.
2006, 31(5): 503-504.
Abstract:
Objective: To analyze the causes of hostpital readmission in patients underwent prostatectomy.Methods: The clinical data of 13 patients who were rehospitalized after operation of prostatectomy were analyzed retrospectively.Results: Dysuria was found in 9 cases,of which 5 were bladder neck restriction,3 were posterior urethal stricture,and 1 was remained glands.One case had stones in the bladder and 3 cases had epididymitis.Conclusions: Posterior urethal stricture,bladder neck restriction and epididymitis are the main causes of readmission in patients who have received prostatectomy.
Objective: To analyze the causes of hostpital readmission in patients underwent prostatectomy.Methods: The clinical data of 13 patients who were rehospitalized after operation of prostatectomy were analyzed retrospectively.Results: Dysuria was found in 9 cases,of which 5 were bladder neck restriction,3 were posterior urethal stricture,and 1 was remained glands.One case had stones in the bladder and 3 cases had epididymitis.Conclusions: Posterior urethal stricture,bladder neck restriction and epididymitis are the main causes of readmission in patients who have received prostatectomy.
2006, 31(5): 505-506.
Abstract:
Objective: To investigate the causes and methods of reoperation after cholecystectomy as well as its prevention.Methods: The causes and methods of reoperation in 9 patients underwent cholecystectomy were analyzed.Results: The main causes were residual cholelithiasis(6/9),hemorrhage in the abdominal cavity(1/9),choledochus injury(1/9),and bile leakage(1/9).Different reoperation methods were employed according to causes of complications.Nine patients were all recovered with good curative effect.Conclusions: A reasonable method is crucial to the treatment of cholecystectomy.Almost all reoperations can be prevented through preoperative evaluation and application of variety of methods in operation.
Objective: To investigate the causes and methods of reoperation after cholecystectomy as well as its prevention.Methods: The causes and methods of reoperation in 9 patients underwent cholecystectomy were analyzed.Results: The main causes were residual cholelithiasis(6/9),hemorrhage in the abdominal cavity(1/9),choledochus injury(1/9),and bile leakage(1/9).Different reoperation methods were employed according to causes of complications.Nine patients were all recovered with good curative effect.Conclusions: A reasonable method is crucial to the treatment of cholecystectomy.Almost all reoperations can be prevented through preoperative evaluation and application of variety of methods in operation.
2006, 31(5): 507-508.
Abstract:
Objective: To investigate the effects,surgical method and complications of excision of cervical and broad ligament myoma.Methods: The clinical data of ten cases with cervical and broad ligament myoma were retrospectively analyzed.Results: Ten cases were entirely cured with surgical excision of myoma.There was no injury of the tissue and organs next to the tumor.However,severe bleeding occurred in two cases with large broad ligament myoma during operation.Conclusions: Surgical excision is still the main therapeutic method for the patient with large broad ligament myoma.It is very important for preventing ureter and artery from injury to identify the relationship between myoma and adjoining tissue.
Objective: To investigate the effects,surgical method and complications of excision of cervical and broad ligament myoma.Methods: The clinical data of ten cases with cervical and broad ligament myoma were retrospectively analyzed.Results: Ten cases were entirely cured with surgical excision of myoma.There was no injury of the tissue and organs next to the tumor.However,severe bleeding occurred in two cases with large broad ligament myoma during operation.Conclusions: Surgical excision is still the main therapeutic method for the patient with large broad ligament myoma.It is very important for preventing ureter and artery from injury to identify the relationship between myoma and adjoining tissue.
2006, 31(5): 508-510.
Abstract:
Objective: To summarize the experience in treating patients with severe craniocerebral injury.Methods: A total of 120 patients with severe craniocerebral injury(GCS<8) treated at our department from July 1997 to July 2005 were retrospectively analyzed.Among these patients,56 underwent operations while 64 were conservatively treated.Results: According to Glasgow outcome score(GOS),24 patients had good results,17 patients had medium nerve function defect,39 patients had serious nerve function defect,38 patients died,and 2 patients were in vegetative state.Conclusions: The prognosis can be improved and the mortality can be decreased if the standardize treatment of patients with severe craniocerebral injury is reinforced.
Objective: To summarize the experience in treating patients with severe craniocerebral injury.Methods: A total of 120 patients with severe craniocerebral injury(GCS<8) treated at our department from July 1997 to July 2005 were retrospectively analyzed.Among these patients,56 underwent operations while 64 were conservatively treated.Results: According to Glasgow outcome score(GOS),24 patients had good results,17 patients had medium nerve function defect,39 patients had serious nerve function defect,38 patients died,and 2 patients were in vegetative state.Conclusions: The prognosis can be improved and the mortality can be decreased if the standardize treatment of patients with severe craniocerebral injury is reinforced.
2006, 31(5): 511-512.
Abstract:
Objective: To explore the relationship between iodinate oil filling shape and the clinical effect after chemoembolization for liver cancer.Methods: Sixty cases of primary hepatocellular carcinoma underwent radiography and transcatheter iodinate oil chemoembolization(TOCE) via proper hepatic artery.CT scan was performed four to six weeks after TOCE.The shape of iodinate oil concentration and its relationship with the survival rate were analyzed.Results: The iodinate oil filling shape after chemoembolization for liver cancer after TOCE could be classified into four types according to the distributive limits and deposition extent of iodized oil:complete type,defective type,aggregated type and sporadic type,the 2-year survival rates of these four types were 69.2%,57.9%,(20.0%) and 0/8,respectively.Conclusions: Clinical therapeutic effect is closely related to the iodinate oil morphology.The more iodinate oil is filled,the higher the survival rate will be.
Objective: To explore the relationship between iodinate oil filling shape and the clinical effect after chemoembolization for liver cancer.Methods: Sixty cases of primary hepatocellular carcinoma underwent radiography and transcatheter iodinate oil chemoembolization(TOCE) via proper hepatic artery.CT scan was performed four to six weeks after TOCE.The shape of iodinate oil concentration and its relationship with the survival rate were analyzed.Results: The iodinate oil filling shape after chemoembolization for liver cancer after TOCE could be classified into four types according to the distributive limits and deposition extent of iodized oil:complete type,defective type,aggregated type and sporadic type,the 2-year survival rates of these four types were 69.2%,57.9%,(20.0%) and 0/8,respectively.Conclusions: Clinical therapeutic effect is closely related to the iodinate oil morphology.The more iodinate oil is filled,the higher the survival rate will be.
2006, 31(5): 513-515.
Abstract:
Objective: To explore the indications for total gastrectomy in treatment of cardiac carcinoma and the procedure of reconstruction of the digestive tract.Methods: Sixty-five patients with cardiac cancer underwent esophagus-jejunum Roux-en-Y reconstruction(RYR)(36 patients) or esophagus-jejunum tip-side anastomosis(TSR)(29 patients) after total gastrectomy.Results: Death in operation and anastomotic leakage occurred in no patients.The post-operative 1-year and 2-year survival rates were 58.5% and 35.4%,respectively.RYR group was better than TSR group in the intestinal volume and time in the intestine,also in the incidence rate of the reflux esophagitis.Conclusions: The patients with the advanced cardiac cancer are in good condition and the total gastrectomy surgical treatment should be employed actively.In addition,total gastrectomy is suitable for patients with small stomach or cardiac cancer following gastrectomy.Esophagus-jejunum Roux-en-Y reconstruction with better curative effect is better than esophagus-jejunum tip-side anastomosis.
Objective: To explore the indications for total gastrectomy in treatment of cardiac carcinoma and the procedure of reconstruction of the digestive tract.Methods: Sixty-five patients with cardiac cancer underwent esophagus-jejunum Roux-en-Y reconstruction(RYR)(36 patients) or esophagus-jejunum tip-side anastomosis(TSR)(29 patients) after total gastrectomy.Results: Death in operation and anastomotic leakage occurred in no patients.The post-operative 1-year and 2-year survival rates were 58.5% and 35.4%,respectively.RYR group was better than TSR group in the intestinal volume and time in the intestine,also in the incidence rate of the reflux esophagitis.Conclusions: The patients with the advanced cardiac cancer are in good condition and the total gastrectomy surgical treatment should be employed actively.In addition,total gastrectomy is suitable for patients with small stomach or cardiac cancer following gastrectomy.Esophagus-jejunum Roux-en-Y reconstruction with better curative effect is better than esophagus-jejunum tip-side anastomosis.
2006, 31(5): 515-516.
Abstract:
Objective: To investigate the clinical efficacy of loop electrosurgical excision procedure(LEEP) in treatment of cervical lesions.Methods: Thirty-one patients with different cervical diseases were diagnosed by optical observation,cytology,colposcopy and cervical biopsy.The lesions were treated with LEEP.The cure rate,the operative time,bleeding volume and postoperative effects were recorded.Results: The cure rate of LEEP was 96.78%.The mean operative time was 4.65 minutes.The mean bleeding volume was 4.97 ml.No secondary bleeding and postoperative infection occurred.All these surgical specimens were examined and the diagnosis was confirmed by pathology.Conclusions: The LEEP in management of the cervical diseases is simple and safe with short operative time,less bleeding and vaginal discharge.It is the best method in treatment of cervical pathological lesions.
Objective: To investigate the clinical efficacy of loop electrosurgical excision procedure(LEEP) in treatment of cervical lesions.Methods: Thirty-one patients with different cervical diseases were diagnosed by optical observation,cytology,colposcopy and cervical biopsy.The lesions were treated with LEEP.The cure rate,the operative time,bleeding volume and postoperative effects were recorded.Results: The cure rate of LEEP was 96.78%.The mean operative time was 4.65 minutes.The mean bleeding volume was 4.97 ml.No secondary bleeding and postoperative infection occurred.All these surgical specimens were examined and the diagnosis was confirmed by pathology.Conclusions: The LEEP in management of the cervical diseases is simple and safe with short operative time,less bleeding and vaginal discharge.It is the best method in treatment of cervical pathological lesions.
2006, 31(5): 517-518.
Abstract:
Objective: To investigate the changes of the serum level of C-reactive protein(CRP) in patients with acute cerebral infarction and its relationship to the severity of cerebral infarction.Methods: The serum level of CRP in 63 patients and 24 healthy persons was determined.All the patients were scored by clinic neurological deficit scale(NDS),and compared in groups.Results: The serum level of CRP in the patients was significantly higher than that in normal controls(P<0.01) and it was significantly higher in the serious group than in the middle and mild groups(P<0.01).Conclusions: The serum level of CRP is closely correlated with the severity of cerebral infarction.
Objective: To investigate the changes of the serum level of C-reactive protein(CRP) in patients with acute cerebral infarction and its relationship to the severity of cerebral infarction.Methods: The serum level of CRP in 63 patients and 24 healthy persons was determined.All the patients were scored by clinic neurological deficit scale(NDS),and compared in groups.Results: The serum level of CRP in the patients was significantly higher than that in normal controls(P<0.01) and it was significantly higher in the serious group than in the middle and mild groups(P<0.01).Conclusions: The serum level of CRP is closely correlated with the severity of cerebral infarction.
2006, 31(5): 519-520.
Abstract:
Objective: To investigate the operative treatment of gluteal muscles contracture,the prevention and the cure of complications.Methods: According to the classification degree of gluteal muscles contracture and the diagnosis criteria of severe cases made by Liu Guohui et al,the diagnosed 287 patients underwent the operation,and the postoperative function exercise.The patients were followed up.Results: The follow up was 4.5 years in average ranged from eleven months to eight years.The effective rate was 100% and the cure rate was 97.5% with less complication and without recurrence.Conclusions: The operation can greatly improve the curative effect of gluteal muscles contracture and reduce postoperative complications to release the pathological tissues completely with less trauma and perform effective function exercise.
Objective: To investigate the operative treatment of gluteal muscles contracture,the prevention and the cure of complications.Methods: According to the classification degree of gluteal muscles contracture and the diagnosis criteria of severe cases made by Liu Guohui et al,the diagnosed 287 patients underwent the operation,and the postoperative function exercise.The patients were followed up.Results: The follow up was 4.5 years in average ranged from eleven months to eight years.The effective rate was 100% and the cure rate was 97.5% with less complication and without recurrence.Conclusions: The operation can greatly improve the curative effect of gluteal muscles contracture and reduce postoperative complications to release the pathological tissues completely with less trauma and perform effective function exercise.
2006, 31(5): 521-522.
Abstract:
Objective: To assess the efficacy and toxicity of a combination therapy of continuous venous infusion(civ) of low-dose of 5-fluorouracil(5-Fu)and cisplatin in treatment of advanced gastric cancer.Methods: The regimen consisted of 5-Fu 250 mg·m-2·d-1 civ,and PDD 6 mg·m-2·d-1,5 d/w,which were used for 3 weeks.The effect and the toxicity were evaluated after 2 cycles of treatment.Results: Gross efficiency rate was 52.8% in 1 the case of CR and 18 cases of PR.The Major toxic effect was gastrointestinal tract reaction.Repression of marrow was light.Conclusions: The chemotherapy regimen mentioned above is effective and has little toxic effect.It is suitable for treatment of advanced gastric cancer.
Objective: To assess the efficacy and toxicity of a combination therapy of continuous venous infusion(civ) of low-dose of 5-fluorouracil(5-Fu)and cisplatin in treatment of advanced gastric cancer.Methods: The regimen consisted of 5-Fu 250 mg·m-2·d-1 civ,and PDD 6 mg·m-2·d-1,5 d/w,which were used for 3 weeks.The effect and the toxicity were evaluated after 2 cycles of treatment.Results: Gross efficiency rate was 52.8% in 1 the case of CR and 18 cases of PR.The Major toxic effect was gastrointestinal tract reaction.Repression of marrow was light.Conclusions: The chemotherapy regimen mentioned above is effective and has little toxic effect.It is suitable for treatment of advanced gastric cancer.
2006, 31(5): 523-524.
Abstract:
Objective: To explore the clinical features and treatment of severe lateral frontal orbit traffic injury.Methods: Clinical data of 27 such patients treated from June 1998 to August 2002 were retrospectively analyzed.Results: The injuries caused by traffic blunt force impact were located on one side of frontal orbit,with confined but complicated wounds.The sufferers had severe open injury,disturbed by slight consciousness disorder with many eye complications.The prospect of mental disorder was obvious.All had mild combined general injury with few contrecoup contusion of brain located in the frontal and temporal lobes and with intracerebral hematoma as well as peridural and subdural hematoma confined to frontotemporale and anterior basocranium.The optic canal fracture and exophthalmus were also commonly seen.Conclusions: Lateral frontal orbit traffic injury is characteristic of high incidence.Its characteristics are related to physiological-anatomic ones in terms of types and impacted areas in traffic injury.It is required in phase I operation that thorough debridement be performed,intracranial hematoma and fragments of necrotic brain tissues be removed in order to prevent cerebral hernia,reduction of fractured frontal orbit be conducted,optic canal be depressurized to save vision and leakage of cerebrospinal fluid be repaired to avoid intracranial infection.
Objective: To explore the clinical features and treatment of severe lateral frontal orbit traffic injury.Methods: Clinical data of 27 such patients treated from June 1998 to August 2002 were retrospectively analyzed.Results: The injuries caused by traffic blunt force impact were located on one side of frontal orbit,with confined but complicated wounds.The sufferers had severe open injury,disturbed by slight consciousness disorder with many eye complications.The prospect of mental disorder was obvious.All had mild combined general injury with few contrecoup contusion of brain located in the frontal and temporal lobes and with intracerebral hematoma as well as peridural and subdural hematoma confined to frontotemporale and anterior basocranium.The optic canal fracture and exophthalmus were also commonly seen.Conclusions: Lateral frontal orbit traffic injury is characteristic of high incidence.Its characteristics are related to physiological-anatomic ones in terms of types and impacted areas in traffic injury.It is required in phase I operation that thorough debridement be performed,intracranial hematoma and fragments of necrotic brain tissues be removed in order to prevent cerebral hernia,reduction of fractured frontal orbit be conducted,optic canal be depressurized to save vision and leakage of cerebrospinal fluid be repaired to avoid intracranial infection.
2006, 31(5): 525-526.
Abstract:
Objective: To investigate the cause of residual gallstones after cholecystectomy as well as the prevention and treatment method.Methods: Fourteen patients with residual stone after cholecystectomy were retrospectively reviewed.Results: The missed diagnosis of gallstone before operation was found in 12 patients and the iatrogenic residual stone was found in two patients.Conclusions: Patients must be examined carefully before operation and several methods of exploration of common bile duct can avoid secondary operation.
Objective: To investigate the cause of residual gallstones after cholecystectomy as well as the prevention and treatment method.Methods: Fourteen patients with residual stone after cholecystectomy were retrospectively reviewed.Results: The missed diagnosis of gallstone before operation was found in 12 patients and the iatrogenic residual stone was found in two patients.Conclusions: Patients must be examined carefully before operation and several methods of exploration of common bile duct can avoid secondary operation.
2006, 31(5): 527-528.
Abstract:
Objective: To evaluate the clinical outcome of transurethral urethrotomy and open operation for urethral stricture.Methods: The data of 103 patients with urethral stricture undergoing the transurethral urethrotomy and the open operation from 1984 to 2005 were reviewed.Results: Among the 65 patients underweut open internal urethrotomy,89.4% were cured,and among the 28 patients underwent posterior urethral anastomosis,92.7% were cured.Conclusions: The treatment for urethral stricture depends on location of urethral stricture,length of the urethral stricture,hardness of scar and state of the body.The open internal urethrotomy is believed to be the first choice of treatment in many patients.
Objective: To evaluate the clinical outcome of transurethral urethrotomy and open operation for urethral stricture.Methods: The data of 103 patients with urethral stricture undergoing the transurethral urethrotomy and the open operation from 1984 to 2005 were reviewed.Results: Among the 65 patients underweut open internal urethrotomy,89.4% were cured,and among the 28 patients underwent posterior urethral anastomosis,92.7% were cured.Conclusions: The treatment for urethral stricture depends on location of urethral stricture,length of the urethral stricture,hardness of scar and state of the body.The open internal urethrotomy is believed to be the first choice of treatment in many patients.
2006, 31(5): 529-531.
Abstract:
Objective: To explore the effectiveness and operative technique of microsurgery through lateral fissure approach for treating hypertensive hemorrhage of basal nuclei region(HBNH).Methods: Twenth-two patients with HBNH accepted at the early stage the microsurgery through small skull window behind pterion and lateral fissure approach to remove the hematoma after they were diagnosed by CT that the hematoma volume is 30-70 ml and no cerebral hernia occurred.Results: The CT reexamination within postoperative 12 hours showed that the hematoma was removed by over 80% were 77.2%,below 70%in 1 case;Postoperative rebleeding(<10 ml) occurred in 1 case;After 6 months follow-up,the ADL was good were 72.7% and the mortality was 13.6%.Conclusions: With proper case selection,microsurgery through lateral fissure approach is a kind of minimal invasive method with little damage,good exposure,comparatively complete hematoma removal,desirable effect of decompressing the intracerebral pressure and stopping the blood flow,favorable recovery of neural functions and low morbidity and mortality.
Objective: To explore the effectiveness and operative technique of microsurgery through lateral fissure approach for treating hypertensive hemorrhage of basal nuclei region(HBNH).Methods: Twenth-two patients with HBNH accepted at the early stage the microsurgery through small skull window behind pterion and lateral fissure approach to remove the hematoma after they were diagnosed by CT that the hematoma volume is 30-70 ml and no cerebral hernia occurred.Results: The CT reexamination within postoperative 12 hours showed that the hematoma was removed by over 80% were 77.2%,below 70%in 1 case;Postoperative rebleeding(<10 ml) occurred in 1 case;After 6 months follow-up,the ADL was good were 72.7% and the mortality was 13.6%.Conclusions: With proper case selection,microsurgery through lateral fissure approach is a kind of minimal invasive method with little damage,good exposure,comparatively complete hematoma removal,desirable effect of decompressing the intracerebral pressure and stopping the blood flow,favorable recovery of neural functions and low morbidity and mortality.
2006, 31(5): 532-533.
Abstract:
Objective: To compare the analgesic effects of propofol and nitric oxide on painless artificial abortion.Methods: Two hundred women with early pregnancy were randomly divided into two groups.Propofol combined with a little fentanyl was used in the observation group while 50% nitric oxide combined with 50% oxygen was used in the control group.The effects of analgesia,cervix relaxation,the occurrence of artificial abortion syndrome and bleeding during the operation were observed.Results: Both propofol and nitric oxide had good effect of analgesia and cervix relaxation.Propofol had a better effect than nitric oxide in artificial abortion(P<0.05).There was no significant difference in the occurrence of artificial abortion syndrome and the quantity of bleeding between the two groups(P>0.05).The time of the mobilization of the patients in the observation group is significantly later than that in the control group(P<0.001).Conclusions: Both propofol and nitric oxide have a good effect of analgesia in the induced abortion.Propofol is better than nitric oxide.
Objective: To compare the analgesic effects of propofol and nitric oxide on painless artificial abortion.Methods: Two hundred women with early pregnancy were randomly divided into two groups.Propofol combined with a little fentanyl was used in the observation group while 50% nitric oxide combined with 50% oxygen was used in the control group.The effects of analgesia,cervix relaxation,the occurrence of artificial abortion syndrome and bleeding during the operation were observed.Results: Both propofol and nitric oxide had good effect of analgesia and cervix relaxation.Propofol had a better effect than nitric oxide in artificial abortion(P<0.05).There was no significant difference in the occurrence of artificial abortion syndrome and the quantity of bleeding between the two groups(P>0.05).The time of the mobilization of the patients in the observation group is significantly later than that in the control group(P<0.001).Conclusions: Both propofol and nitric oxide have a good effect of analgesia in the induced abortion.Propofol is better than nitric oxide.
2006, 31(5): 534-535.
Abstract:
Objective: To explore operative methods for female pseudohermaphroditism caused by extraneous androgen.Methods: According to characteristics of vuval malformation,plastic operation of clitoris,greater and smaller lips of pudendum,prolonged urethra and lower segmental vagina were performed respectively and female vulva was restored.Results: Operations on 13 patients were all successful.Female vulva was recovered.Vagina could hold two fingers.External open position of urethra was normal.Sexual life was satisfactory after marriage.Conclusions: Various corrections were designed according to characteristics of vuval malformation.Autografts are fully used to make the anatomical structure and physiological functions tend to be normal after correction.
Objective: To explore operative methods for female pseudohermaphroditism caused by extraneous androgen.Methods: According to characteristics of vuval malformation,plastic operation of clitoris,greater and smaller lips of pudendum,prolonged urethra and lower segmental vagina were performed respectively and female vulva was restored.Results: Operations on 13 patients were all successful.Female vulva was recovered.Vagina could hold two fingers.External open position of urethra was normal.Sexual life was satisfactory after marriage.Conclusions: Various corrections were designed according to characteristics of vuval malformation.Autografts are fully used to make the anatomical structure and physiological functions tend to be normal after correction.
2006, 31(5): 536-537.
Abstract:
Objective: To investigate the effect of postterm pregnancy on the prognosis of the pregnant women and the perinatal children.Methods: The records of 4 383 multiparas from January 2001 to July 2005 were retrospectively analyzed.Results: The incidence of caesarean section,the postpartum hemorrhage,the oligohydramnios,the fetal macrosomia,the fetal distress and the perinatal death in postterm pregnancy group were higher than those in the term pregnancy group(P<0.01 and P=0.000).Conclusions: The postterm pregnancy is very harmful to the pregnant women and the perinatal children.In order to reduce the incidence of postterm pregnancy and improve the prognosis of the pregnant women and the perinatal children,the pregnancy should be terminated in time.
Objective: To investigate the effect of postterm pregnancy on the prognosis of the pregnant women and the perinatal children.Methods: The records of 4 383 multiparas from January 2001 to July 2005 were retrospectively analyzed.Results: The incidence of caesarean section,the postpartum hemorrhage,the oligohydramnios,the fetal macrosomia,the fetal distress and the perinatal death in postterm pregnancy group were higher than those in the term pregnancy group(P<0.01 and P=0.000).Conclusions: The postterm pregnancy is very harmful to the pregnant women and the perinatal children.In order to reduce the incidence of postterm pregnancy and improve the prognosis of the pregnant women and the perinatal children,the pregnancy should be terminated in time.
2006, 31(5): 538-539.
Abstract:
Objective: To explore the effect of internal fixation of fibula combined with small-incision reduction fixation and external fixative frame in treatment of fractures of tibia and fibula.Methods: Thirty-six patients with fractures of tibia and fibula were treated by internal fixation of fibula combined with small-incision reduction fixation and external frame from January 2002 through April 2005.Results: During the follow-up period from 6 to 34 months in thirty-six patients,according to the criteria of Johner and Wruh,twenty patients were assessed as excellent,nine as good,five as fair and two as poor.Conclusions: The combined operative procedure is simple and reliable and the functional recovery is satisfactory.
Objective: To explore the effect of internal fixation of fibula combined with small-incision reduction fixation and external fixative frame in treatment of fractures of tibia and fibula.Methods: Thirty-six patients with fractures of tibia and fibula were treated by internal fixation of fibula combined with small-incision reduction fixation and external frame from January 2002 through April 2005.Results: During the follow-up period from 6 to 34 months in thirty-six patients,according to the criteria of Johner and Wruh,twenty patients were assessed as excellent,nine as good,five as fair and two as poor.Conclusions: The combined operative procedure is simple and reliable and the functional recovery is satisfactory.
2006, 31(5): 564-565.
Abstract:
Objective: To compare the results of serum HBsAg detection by eletrochemiluminescence immunoassay(ECLIA) and enzyme-linked immunobsorbent assay(ELISA).Methods: ECLIA and one-step and two-step ELISA were used to detect quantitative HBsAg and 99 serum samples which were divided into four models:①HBsAg(+)/HBeAg(+)/HBcAb(+),②HBsAg(+)/HBeAb(+)/HBcAb(+),③HBsAg(+)/HBcAb(+),④HBeAg(+)/HBcAb(+).Results: The within-day CV by ECLIA increased 3.3% and 3.2% compared to by one-step ELISA and two-step ELISA alone;and the between-day CV by ECLIA increased 3.5% and 3.3%,and the sensitivity was 0.063,0.5 and 0.25ng/ml respectively.The positive ratio by ECLIA and one-step,two-step ELISA was 100% in model①②③.The result was positive in two samples of model④by ECLIA 1:50 dilute and two-step ELISA,while the result was negative by ECLIA undiluted and one-step ELISA.In model①,the level of 1:50 diluted HBsAg COI was higher than that of undiluted.Conclusions: One-step ELISA is simple and quick in detecting HBsAg,but too high or too low concentration of the sample may issue false negative.To avoid hook effect,two-step ELISA is recommended.ECLIA has a high sensitivity,but there also exists hook effect.Prediluting samples or Elecsys HBsAg confirmatory test should be adopted when necessary.
Objective: To compare the results of serum HBsAg detection by eletrochemiluminescence immunoassay(ECLIA) and enzyme-linked immunobsorbent assay(ELISA).Methods: ECLIA and one-step and two-step ELISA were used to detect quantitative HBsAg and 99 serum samples which were divided into four models:①HBsAg(+)/HBeAg(+)/HBcAb(+),②HBsAg(+)/HBeAb(+)/HBcAb(+),③HBsAg(+)/HBcAb(+),④HBeAg(+)/HBcAb(+).Results: The within-day CV by ECLIA increased 3.3% and 3.2% compared to by one-step ELISA and two-step ELISA alone;and the between-day CV by ECLIA increased 3.5% and 3.3%,and the sensitivity was 0.063,0.5 and 0.25ng/ml respectively.The positive ratio by ECLIA and one-step,two-step ELISA was 100% in model①②③.The result was positive in two samples of model④by ECLIA 1:50 dilute and two-step ELISA,while the result was negative by ECLIA undiluted and one-step ELISA.In model①,the level of 1:50 diluted HBsAg COI was higher than that of undiluted.Conclusions: One-step ELISA is simple and quick in detecting HBsAg,but too high or too low concentration of the sample may issue false negative.To avoid hook effect,two-step ELISA is recommended.ECLIA has a high sensitivity,but there also exists hook effect.Prediluting samples or Elecsys HBsAg confirmatory test should be adopted when necessary.
2006, 31(5): 566-567.
Abstract:
Objective: To investigate the blood lead levels of traffic police in Suzhou city and the correlative factors.Methods: The blood lead levels of the traffic police in Suzhou and the correlative factors were studied.A mono-factor correlation analysis and multiple stepwise regression analysis were carried out to analyze the possible factors affecting blood lead levels(Ŷ),such as outdoor-on-duty years(X1),hemoglobin(X2),smoking(X3) and blood zinc(X4).Results: The average levels of blood lead and urine lead of the traffic police were 119.79 and 6.99 μg/L respectively,which were significantly higher than those of the controls(88.38 and 5.34(μg/L))(P<0.001 and P<0.01).In mono-factor correlation analysis,the blood lead levels showed a positive correlation with outdoor-on-duty years(P<0.001),and a negative correlation with concentrations of hemoglobin(P<0.001).In multiple stepwise regression analysis,the regression equation was established as Ŷ=132.95+0.93X1-0.86X2+7.37X3+14.30X4.Conclusions: It suggests that the blood lead levels rise with the increase of outdoor-on-duty years,and the synthesis of hemoglobin is inhibited to a certain extent.
Objective: To investigate the blood lead levels of traffic police in Suzhou city and the correlative factors.Methods: The blood lead levels of the traffic police in Suzhou and the correlative factors were studied.A mono-factor correlation analysis and multiple stepwise regression analysis were carried out to analyze the possible factors affecting blood lead levels(Ŷ),such as outdoor-on-duty years(X1),hemoglobin(X2),smoking(X3) and blood zinc(X4).Results: The average levels of blood lead and urine lead of the traffic police were 119.79 and 6.99 μg/L respectively,which were significantly higher than those of the controls(88.38 and 5.34(μg/L))(P<0.001 and P<0.01).In mono-factor correlation analysis,the blood lead levels showed a positive correlation with outdoor-on-duty years(P<0.001),and a negative correlation with concentrations of hemoglobin(P<0.001).In multiple stepwise regression analysis,the regression equation was established as Ŷ=132.95+0.93X1-0.86X2+7.37X3+14.30X4.Conclusions: It suggests that the blood lead levels rise with the increase of outdoor-on-duty years,and the synthesis of hemoglobin is inhibited to a certain extent.
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