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2008 Vol. 33, No. 6
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2008, 33(6): 631-634.
Abstract:
Objective: To investigate the effect of glucocorticoid on expression of hypothalamic Agouti-related peptide(AGRP),and cocaine and amphetamine regulated transcript(CART) in normal and obese rats.Methods: Both normal and obese SD rats were randomly assigned to 3 groups:normal salina group(NSG),low dose glucocorticoid group(LDG) and high dose glucocorticoid group(HDG),and intraperitoneal injection with normal saline 0.2 ml/100 g,hydrocortisone sodium succinate 5 mg/kg and 15 mg/kg for 20 days.Real-time RT-PCR was used to measure the expression of AGRP,CART mRNA in hypothalamus.Results: After high dose glucocorticoid injection,the mRNA expressions of orexigenic neuropeptide AGRP and anorexigenic neuropeptide CART,were significantly lower than the NSG in hypothalamus of both obese and normal rats(P<0.05).After low dose glucocorticoid injection,there were no differences of hypothalamic AGRP,CART mRNA expressions from the NSG in obese rats.In normal rats,the expressions of AGRP mRNA were significantly lower than those of the NSG after low dose glucocorticoid injection(P<0.05) while no difference of CART mRNA from the NSG.Conclusions: High dose glucocorticoid downregulated orexigenic neuropeptide AGRP and anorexigenic neuropeptide CART mRNA expression levels in hypothalamus of obese and normal rats.Low dose glucocorticoid downregulated orexigenic neuropeptide AGRP mRNA expression in hypothalamus of normal rats.
Objective: To investigate the effect of glucocorticoid on expression of hypothalamic Agouti-related peptide(AGRP),and cocaine and amphetamine regulated transcript(CART) in normal and obese rats.Methods: Both normal and obese SD rats were randomly assigned to 3 groups:normal salina group(NSG),low dose glucocorticoid group(LDG) and high dose glucocorticoid group(HDG),and intraperitoneal injection with normal saline 0.2 ml/100 g,hydrocortisone sodium succinate 5 mg/kg and 15 mg/kg for 20 days.Real-time RT-PCR was used to measure the expression of AGRP,CART mRNA in hypothalamus.Results: After high dose glucocorticoid injection,the mRNA expressions of orexigenic neuropeptide AGRP and anorexigenic neuropeptide CART,were significantly lower than the NSG in hypothalamus of both obese and normal rats(P<0.05).After low dose glucocorticoid injection,there were no differences of hypothalamic AGRP,CART mRNA expressions from the NSG in obese rats.In normal rats,the expressions of AGRP mRNA were significantly lower than those of the NSG after low dose glucocorticoid injection(P<0.05) while no difference of CART mRNA from the NSG.Conclusions: High dose glucocorticoid downregulated orexigenic neuropeptide AGRP and anorexigenic neuropeptide CART mRNA expression levels in hypothalamus of obese and normal rats.Low dose glucocorticoid downregulated orexigenic neuropeptide AGRP mRNA expression in hypothalamus of normal rats.
2008, 33(6): 634-636.
Abstract:
Objective: To study the relationship of expressions of survivin and HIF-1α in the tissue of non-small cell lung cancer(NSCLC) with differentiation,lymph node metastasis and clinical stage and the correlation between them.Methods: The expressions of survivin and HIF-1α protein were detected by immunohistochemistry in the specimens of 120 NSCLC samples and 40 benign lung disease samples.Results: The positive rate of survivin in NSCLC was 81.60%,and there was no expression in the tissue of benign lung disease.The positive rate of HIF-1α was 58.33%,higher than that in the tissue of benign disease,which was 10.00%;both the expressions of survivin and HIF-1α had relationship with differentiation,lymph node metastasis and clinical stage,and the expression of survivin had positive relation with the expression of HIF-1α in NSCLC(P=0.005).Conclusions: The expressions of survivin and HIF-1α were high and they both have relationship with differentiation,lymph node metastasis and clinical stage of NSCLC,and the expression of HIF-1α protein had positively correlation with expression of survivin protein.
Objective: To study the relationship of expressions of survivin and HIF-1α in the tissue of non-small cell lung cancer(NSCLC) with differentiation,lymph node metastasis and clinical stage and the correlation between them.Methods: The expressions of survivin and HIF-1α protein were detected by immunohistochemistry in the specimens of 120 NSCLC samples and 40 benign lung disease samples.Results: The positive rate of survivin in NSCLC was 81.60%,and there was no expression in the tissue of benign lung disease.The positive rate of HIF-1α was 58.33%,higher than that in the tissue of benign disease,which was 10.00%;both the expressions of survivin and HIF-1α had relationship with differentiation,lymph node metastasis and clinical stage,and the expression of survivin had positive relation with the expression of HIF-1α in NSCLC(P=0.005).Conclusions: The expressions of survivin and HIF-1α were high and they both have relationship with differentiation,lymph node metastasis and clinical stage of NSCLC,and the expression of HIF-1α protein had positively correlation with expression of survivin protein.
2008, 33(6): 637-639.
Abstract:
Objective: To study on differentiation of marrow-derived endothelial progenitor cells(EPCs) in vivo,and to further reveal EPCs' biological characteristics.Methods: Rat bone marrow-derived EPCs were separated by density gradient centrifugation,and cultrued in vitro;and then labelled with Hoechst 33342 and collected.The labelled EPCs were injected subcutaneously into nude mice's back,on the both sides of the spinal.Animals were observed daily and measured the size of the subcutaneous masses.At the 50th day after transplantation,the subcutaneous masses were removed and then performed HE and immunofluorescent staining to detect the expression of antigens CD31,Flk1,CD34.Results: At the 20th day after transplantation,we observed the subcutaneous masses with size of(0.13±0.018) cm2 on the nude mice's back,and then the subcutaneous masses were gradually increasing.At the 40th day,EPCs began to get into period of growth stagnation.Tube-like structure,muscle tissue and fatty-like cells were observed through HE staining,at the same vision,blue fluorescent-marked cells were observed through fluorescence microscopy.The result of Immunofluorescence staining showed that some of the blue fluorescent cells expressed antigens CD31,Flk1,CD34.Conclusions: The part of the EPCs transplanted to nude mice differentiated into endothelial-like cells and formed tube-like structure,but the other part differentiated into non-endothelial cells.
Objective: To study on differentiation of marrow-derived endothelial progenitor cells(EPCs) in vivo,and to further reveal EPCs' biological characteristics.Methods: Rat bone marrow-derived EPCs were separated by density gradient centrifugation,and cultrued in vitro;and then labelled with Hoechst 33342 and collected.The labelled EPCs were injected subcutaneously into nude mice's back,on the both sides of the spinal.Animals were observed daily and measured the size of the subcutaneous masses.At the 50th day after transplantation,the subcutaneous masses were removed and then performed HE and immunofluorescent staining to detect the expression of antigens CD31,Flk1,CD34.Results: At the 20th day after transplantation,we observed the subcutaneous masses with size of(0.13±0.018) cm2 on the nude mice's back,and then the subcutaneous masses were gradually increasing.At the 40th day,EPCs began to get into period of growth stagnation.Tube-like structure,muscle tissue and fatty-like cells were observed through HE staining,at the same vision,blue fluorescent-marked cells were observed through fluorescence microscopy.The result of Immunofluorescence staining showed that some of the blue fluorescent cells expressed antigens CD31,Flk1,CD34.Conclusions: The part of the EPCs transplanted to nude mice differentiated into endothelial-like cells and formed tube-like structure,but the other part differentiated into non-endothelial cells.
2008, 33(6): 640-643.
Abstract:
Objective: To construct recombinant plasmid with fusion gene Tat47-57-HBcAg,express fusion protein Tat47-57-HBcAg in E.coli and identification the Tat47-57-HBcAg by western blot analysis.Methods: To synthesize the sequence of Tat47-57 and amplify HBcAg gene by PCR,splice the two sequences with splicing by overlap extension PCR,link fusion gene into pET28a,the sequences correct vector be transformed into E.coli Rosetta-gamiTM 2(DE3),then the transformed E.coli is induced by isopropyl β-D-1-Thiogalactopyranoside and the expression product is analyzed by SDS-PAGE,furthermore,identification the expression product by Western blot with HBcAg monoclonal antibody.Results: Fusion protein Tat47-57-HBcAg is highly effective expressed in E.coli Rosetta-gamiTM 2(DE3).The solubility analysis of expression product indicated that the fusion protein are mostly expressed in supernatant and expression product could react with HBcAg monoclonal antibody by western blot analysis.Conclusions: The Tat47-57-HBcAg fusion protein can be expressed in E.coli Rossetta-gami 2 correctly as soluble protein and be recognized by HBcAg monoclonal antibody.
Objective: To construct recombinant plasmid with fusion gene Tat47-57-HBcAg,express fusion protein Tat47-57-HBcAg in E.coli and identification the Tat47-57-HBcAg by western blot analysis.Methods: To synthesize the sequence of Tat47-57 and amplify HBcAg gene by PCR,splice the two sequences with splicing by overlap extension PCR,link fusion gene into pET28a,the sequences correct vector be transformed into E.coli Rosetta-gamiTM 2(DE3),then the transformed E.coli is induced by isopropyl β-D-1-Thiogalactopyranoside and the expression product is analyzed by SDS-PAGE,furthermore,identification the expression product by Western blot with HBcAg monoclonal antibody.Results: Fusion protein Tat47-57-HBcAg is highly effective expressed in E.coli Rosetta-gamiTM 2(DE3).The solubility analysis of expression product indicated that the fusion protein are mostly expressed in supernatant and expression product could react with HBcAg monoclonal antibody by western blot analysis.Conclusions: The Tat47-57-HBcAg fusion protein can be expressed in E.coli Rossetta-gami 2 correctly as soluble protein and be recognized by HBcAg monoclonal antibody.
2008, 33(6): 644-647.
Abstract:
Objective: To clone genes of encoding porcine interleukin-12 p35 and p40 subunits,for preparing the compound nucleir acid vaccine against the porcine bladder worm.Methods: Peripheral blood mononuclear cells and splenic lymphocytes of porcine were isolated and cultured for 10 hours,and stimulated with 100 ng/ml IFN-γ for 12 hours,followed by stimulated with 1 μg/ml lipopolysaccharide for 3 hours.Total RNA from these stimulated porcine lymphocytes were extracted,cDNA of genes for porcine IL-12 p35 and p40 subunit were ampliphied by RT-PCR assay and inserted into pMD18-T vector.The cloned genes were sequenced.Results: The PCR products of porcine IL-12 p35 and p40 subunits were 616 bp and 1 011 bp,respectively,verified by electrophoresis,sequence analysis for cloned genes in pMD18-T vector showed one base was different in each but none affecting amino acids coding,confirmed to be genes of porcine IL-12 p35 and p40 subunit.Conclusions: cDNA encoding porcine interleukin-12 p35 and p40 subunit have been successfully cloned into cloning vector pMD18-T.
Objective: To clone genes of encoding porcine interleukin-12 p35 and p40 subunits,for preparing the compound nucleir acid vaccine against the porcine bladder worm.Methods: Peripheral blood mononuclear cells and splenic lymphocytes of porcine were isolated and cultured for 10 hours,and stimulated with 100 ng/ml IFN-γ for 12 hours,followed by stimulated with 1 μg/ml lipopolysaccharide for 3 hours.Total RNA from these stimulated porcine lymphocytes were extracted,cDNA of genes for porcine IL-12 p35 and p40 subunit were ampliphied by RT-PCR assay and inserted into pMD18-T vector.The cloned genes were sequenced.Results: The PCR products of porcine IL-12 p35 and p40 subunits were 616 bp and 1 011 bp,respectively,verified by electrophoresis,sequence analysis for cloned genes in pMD18-T vector showed one base was different in each but none affecting amino acids coding,confirmed to be genes of porcine IL-12 p35 and p40 subunit.Conclusions: cDNA encoding porcine interleukin-12 p35 and p40 subunit have been successfully cloned into cloning vector pMD18-T.
2008, 33(6): 648-651.
Abstract:
Objective: To observe resistant-drug bacteria and their genotype of ESBLs in K.pneumoniae and E.coli from clinical specimens and to understand their epidemiological status and the mechanisms of drug-resistant to antibiotics.Methods: The isolation,cultivation and identification of bacteria were performed by the traditional procedures.Microdilution and K-B method were applied to detect the resistance of ESBL-producing in K.pneumoniae and E.coli to cefoperazone/sulbactam.ESBLs were confirmed by double disk test.Polymerase chain reaction(PCR) was applied to amplify the TEM and SHV genotype of beta-lactamase.Results: The resistance rates to common antibiotics were up to 90% in K.pneumoniae and E.coli,including to AMP,AMP/SB,PIP,CF,CFZ,CRM,CPD,CAX,CAZ,CTX,CPE and AZT.Furthermore,the resistance was multiple resistant-drug bacteria except for sensitive to imipenem.There-fore,the carbopenems was main antibiotics to bacteria producing ESBLs.The percentage of the genotype of TEM or SHV or TEM and SHV of the bacteria-producing ESBLs in K.pneumoniae were 26.92%,30.76%,7.69%,respectively,and were 46.15%,5.12%,10.25% in E.coli,respectively.Conclusions: The resistance rates are high to lots of common antibiotics in K.pneumoniae and E.coli,especially to penicillin and cephalosporin except for sensitive to imipenem,the carbopenems are main antibiotics to bacteria-producing ESBLs.Most of ESBLs-producting bacteria carry the genotype of TEM or SHV.The SHV genotype of β-lactamase is mainly in K.pneumoniae,TEM genotype of β-lactamase is found mainly in E.coli.
Objective: To observe resistant-drug bacteria and their genotype of ESBLs in K.pneumoniae and E.coli from clinical specimens and to understand their epidemiological status and the mechanisms of drug-resistant to antibiotics.Methods: The isolation,cultivation and identification of bacteria were performed by the traditional procedures.Microdilution and K-B method were applied to detect the resistance of ESBL-producing in K.pneumoniae and E.coli to cefoperazone/sulbactam.ESBLs were confirmed by double disk test.Polymerase chain reaction(PCR) was applied to amplify the TEM and SHV genotype of beta-lactamase.Results: The resistance rates to common antibiotics were up to 90% in K.pneumoniae and E.coli,including to AMP,AMP/SB,PIP,CF,CFZ,CRM,CPD,CAX,CAZ,CTX,CPE and AZT.Furthermore,the resistance was multiple resistant-drug bacteria except for sensitive to imipenem.There-fore,the carbopenems was main antibiotics to bacteria producing ESBLs.The percentage of the genotype of TEM or SHV or TEM and SHV of the bacteria-producing ESBLs in K.pneumoniae were 26.92%,30.76%,7.69%,respectively,and were 46.15%,5.12%,10.25% in E.coli,respectively.Conclusions: The resistance rates are high to lots of common antibiotics in K.pneumoniae and E.coli,especially to penicillin and cephalosporin except for sensitive to imipenem,the carbopenems are main antibiotics to bacteria-producing ESBLs.Most of ESBLs-producting bacteria carry the genotype of TEM or SHV.The SHV genotype of β-lactamase is mainly in K.pneumoniae,TEM genotype of β-lactamase is found mainly in E.coli.
2008, 33(6): 652-655.
Abstract:
Objective: To investigate the regulation of transcription factor RORγt on the IFN-γ production in different T cell subpopulations.Methods: Peripheral blood mononuclear cells were isolated from peripheral blood of healthy donors by density gradient centrifugation,and were stimulated with CD3 monoclonal antibody and cultured in IL-2 containing medium for 12 days.The activated T cells were transfected with chemical synthesized small interfering RNA(siRNA) sequences for RORγt gene using liposomes.The expression of RORγt gene was detected in RORγt-siRNA transfected T cells by RT-PCR assay.The percentages of IFN-γ producing cells in total T cells,CD4+ and CD8+ T cells were measured in the transfected T cells by flowcytometry.Results: By using semi-quantity RT-PCR assay,the RORγt gene expression was inhibited in activated T cells that transfected with RORγt-siRNA-1026 and-1197,but not inhibited in that with RORγt-siRNA-982.Among the RORγt-siRNA-1026 transfected activated T cells,the percentage of IFN-γ producing cells in CD4+ T cells(32.78±3.41) significantly decreased,compared to untransfected group(44.54±1.75) (P<0.01).Conclusions: Transcription factor RORγt is involved in the positive regulation for IFN-γ production in CD4+ T cells.
Objective: To investigate the regulation of transcription factor RORγt on the IFN-γ production in different T cell subpopulations.Methods: Peripheral blood mononuclear cells were isolated from peripheral blood of healthy donors by density gradient centrifugation,and were stimulated with CD3 monoclonal antibody and cultured in IL-2 containing medium for 12 days.The activated T cells were transfected with chemical synthesized small interfering RNA(siRNA) sequences for RORγt gene using liposomes.The expression of RORγt gene was detected in RORγt-siRNA transfected T cells by RT-PCR assay.The percentages of IFN-γ producing cells in total T cells,CD4+ and CD8+ T cells were measured in the transfected T cells by flowcytometry.Results: By using semi-quantity RT-PCR assay,the RORγt gene expression was inhibited in activated T cells that transfected with RORγt-siRNA-1026 and-1197,but not inhibited in that with RORγt-siRNA-982.Among the RORγt-siRNA-1026 transfected activated T cells,the percentage of IFN-γ producing cells in CD4+ T cells(32.78±3.41) significantly decreased,compared to untransfected group(44.54±1.75) (P<0.01).Conclusions: Transcription factor RORγt is involved in the positive regulation for IFN-γ production in CD4+ T cells.
2008, 33(6): 656-660.
Abstract:
Objective: To characterize the outer membrane proteins in a Carbapenem resistant Acinetobacter junii(A.junii) clinical isolate and to speculate their roles involved in drug resistance.Methods: A multi-drug,including imipenem,resistant A.junii strain ZN3858 was recovered from the sputum of an acute exacerbation of chronic obstructive pulmonary disease in patient.Species identification was performed by the API 20NE and the combination of ARDRA and RAPD fingerprinting analysis.With the reference of A.junii ATCC 17908,the outer membrane proteins(OMPs) profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and the quantitatively different expressed proteins were selected.Matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOFMS) and PCR were employed to study the characters of the proteins concerned.Results: This carbapenem-resistant A.junii isolates ZN3858 had reduced expression of 35-,and 47-kDa outer-membrane proteins resembling oprD and oprF precursor respectively,which were OMPs associated with antibiotic resistance.Meanwhile,40 kDa outer membrane protein(omp40) was unexpectedly up-regulated,which highly resemble HMB-AB,an imipenem resistance OMP in imipenem resistant A.baumannii.The partial coding DNA sequence(CDS) of omp40 was obtained and the Genbank Accession Number assigned as AY626903.Conclusions: Outer membrane proteins may be involved in multi-drug resistance in A.junii.omp40 in A.junii is probably the counterpart of HMB-AB in A.baumannii,further research is necessary to elucidate its function in imipenem resistance.
Objective: To characterize the outer membrane proteins in a Carbapenem resistant Acinetobacter junii(A.junii) clinical isolate and to speculate their roles involved in drug resistance.Methods: A multi-drug,including imipenem,resistant A.junii strain ZN3858 was recovered from the sputum of an acute exacerbation of chronic obstructive pulmonary disease in patient.Species identification was performed by the API 20NE and the combination of ARDRA and RAPD fingerprinting analysis.With the reference of A.junii ATCC 17908,the outer membrane proteins(OMPs) profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and the quantitatively different expressed proteins were selected.Matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOFMS) and PCR were employed to study the characters of the proteins concerned.Results: This carbapenem-resistant A.junii isolates ZN3858 had reduced expression of 35-,and 47-kDa outer-membrane proteins resembling oprD and oprF precursor respectively,which were OMPs associated with antibiotic resistance.Meanwhile,40 kDa outer membrane protein(omp40) was unexpectedly up-regulated,which highly resemble HMB-AB,an imipenem resistance OMP in imipenem resistant A.baumannii.The partial coding DNA sequence(CDS) of omp40 was obtained and the Genbank Accession Number assigned as AY626903.Conclusions: Outer membrane proteins may be involved in multi-drug resistance in A.junii.omp40 in A.junii is probably the counterpart of HMB-AB in A.baumannii,further research is necessary to elucidate its function in imipenem resistance.
2008, 33(6): 661-664.
Abstract:
Objective: To investigate the changes of the cell cycle of differentiated HL-60 initiated by dimethylsulphoxide(DMSO) and explore the specific phase of the cell differentiation in cell cycle.Methods: HL-60 cells influenced by dimethylsulphoxide capable of inducing differentiation for different time were used as subjects.The cell volume and the differentiation marker on the cell surface were analyzed by flow cytometry.The differentiated cells were identified by confocal microscope after stained with propidium iodide.Meanwhile,The change of the cell cycles induced by those drugs was analyzed by flow cytometry.The expression level of Ki67 in G1 phase was tested by flow cytometry.The specific phase of the drug-induced cell differentiation in cell cycle was explored by flow cytometry sorting combined with confocal microscopic pictures of the different phase cells.Results: When the drug-induced time prolonged,the induced cell volume enlarged and the induced cells began to express CD11b(differentiation marker of myeloid cell).The peak value of G0/G1 phase was elevated in drug-induced cell cell cycle and the level of S phase was declined in drug-induced cell cycle.The expression level of Ki67 in G1 phase was gradually declined.The differentiated cells were only observed in G0/G1 phase.Conclusions: Dimethylsulphoxide can induce HL-60 cell to differentiate along the way to granulocytic cells and induce the arrest of cell cycle in early G1 phase to late G1 phase.The cell differentiation was completed in early G1 phase in cell cycle.
Objective: To investigate the changes of the cell cycle of differentiated HL-60 initiated by dimethylsulphoxide(DMSO) and explore the specific phase of the cell differentiation in cell cycle.Methods: HL-60 cells influenced by dimethylsulphoxide capable of inducing differentiation for different time were used as subjects.The cell volume and the differentiation marker on the cell surface were analyzed by flow cytometry.The differentiated cells were identified by confocal microscope after stained with propidium iodide.Meanwhile,The change of the cell cycles induced by those drugs was analyzed by flow cytometry.The expression level of Ki67 in G1 phase was tested by flow cytometry.The specific phase of the drug-induced cell differentiation in cell cycle was explored by flow cytometry sorting combined with confocal microscopic pictures of the different phase cells.Results: When the drug-induced time prolonged,the induced cell volume enlarged and the induced cells began to express CD11b(differentiation marker of myeloid cell).The peak value of G0/G1 phase was elevated in drug-induced cell cell cycle and the level of S phase was declined in drug-induced cell cycle.The expression level of Ki67 in G1 phase was gradually declined.The differentiated cells were only observed in G0/G1 phase.Conclusions: Dimethylsulphoxide can induce HL-60 cell to differentiate along the way to granulocytic cells and induce the arrest of cell cycle in early G1 phase to late G1 phase.The cell differentiation was completed in early G1 phase in cell cycle.
2008, 33(6): 665-668.
Abstract:
Objective: To construc and identify the expression vector which contains a luciferase gene driven by human telomerase reverse transcriptase(hTERT) gene promotor.Methods: The fragment of hTERT promoter about 1 100 bp was acquired by nested PCR amplification,the correct sequence was inserted into the luciferase gene expression vector.In order to get a lot of containing hTERT promoter luciferase recombinant,the construction of pGL3-hTERTp recombinant was transformed into JM190 bacteria.Restriction endonuclease and PCR were used to identify the recombinant and sequencing.Results: After identifying which used restriction endonuclease and PCR method,we successfully constructed the recombinant of hTERT promoter luciferase gene expression vector.Conclusions: A novel recombinant luciferase gene expression vector driven by hTERT promoter is made successfully,and the newly constructed recombinant is useful for study the molecular mechanism that As2O3 inhibits the expression of telomerase of HL-60 cells.
Objective: To construc and identify the expression vector which contains a luciferase gene driven by human telomerase reverse transcriptase(hTERT) gene promotor.Methods: The fragment of hTERT promoter about 1 100 bp was acquired by nested PCR amplification,the correct sequence was inserted into the luciferase gene expression vector.In order to get a lot of containing hTERT promoter luciferase recombinant,the construction of pGL3-hTERTp recombinant was transformed into JM190 bacteria.Restriction endonuclease and PCR were used to identify the recombinant and sequencing.Results: After identifying which used restriction endonuclease and PCR method,we successfully constructed the recombinant of hTERT promoter luciferase gene expression vector.Conclusions: A novel recombinant luciferase gene expression vector driven by hTERT promoter is made successfully,and the newly constructed recombinant is useful for study the molecular mechanism that As2O3 inhibits the expression of telomerase of HL-60 cells.
2008, 33(6): 668-671.
Abstract:
Objective: To investigate the differentially expressed genes of proliferating hemangiomas and normal tissue by cDNA microarray analysis of gene expression profiles and to identify the key disease related genes.Methods: Samples were processed from total RNA and purified to mRNA,which was reverse transcripted and hybridized onto Biodoor Genechip expression microarrays.Gene expression analysis were performed to determine the differences between the proliferating hemangiomas and the normal tissue.Results: In proliferating hemangioma,122 genes were up-regulated and 127 genes were down-regulated.Those over-expressed gene were mainly related to cell proliferation,angiogenesis,and immune cell adhesion,cell cycle-related protein,while the less-expressed genes were mainly related to cell apoptosis and tumor suppression.Conclusions: Over-expression of genes related to cell proliferation and less-expression of cell apoptosis related genes lead to imbalance between cell proliferation and apoptosis,which probably lead to the occurrence of hemangiomas.
Objective: To investigate the differentially expressed genes of proliferating hemangiomas and normal tissue by cDNA microarray analysis of gene expression profiles and to identify the key disease related genes.Methods: Samples were processed from total RNA and purified to mRNA,which was reverse transcripted and hybridized onto Biodoor Genechip expression microarrays.Gene expression analysis were performed to determine the differences between the proliferating hemangiomas and the normal tissue.Results: In proliferating hemangioma,122 genes were up-regulated and 127 genes were down-regulated.Those over-expressed gene were mainly related to cell proliferation,angiogenesis,and immune cell adhesion,cell cycle-related protein,while the less-expressed genes were mainly related to cell apoptosis and tumor suppression.Conclusions: Over-expression of genes related to cell proliferation and less-expression of cell apoptosis related genes lead to imbalance between cell proliferation and apoptosis,which probably lead to the occurrence of hemangiomas.
2008, 33(6): 672-676.
Abstract:
Objective: To investigate the effects of bacteria motif,namely CpG oligodeoxynucleotide(CpG-ODN) on morphology and melanogenesis production of and prostaglandin E2(PGE2) in melanocytes.Methods: The cultured B16 melanoma cells and melan-α melanocytes in vitro were treated with the CpG-ODN.The B16 cell viability,tyrosinase activity,melanin content and the level of PGE2 in the culture supernatants was measured by MTT assay,oxidation of L-dopa,NaOH assay and ELISA assay respectively.In addition,the morphological changes of melan-α cells were observed after the CpG-ODN was added.Results: The CpG-ODN at the concentration of 5 μg/ml promoted the proliferation of B16 cells,10 μg/ml with little effects,and 15 μg/ml with depressant effects on the cell proliferation after drugs intervention for 24 h.After 48 h treating,the cell proliferation rates in various groups became stable and were similar to each other,without significant difference between groups at different concentrations of CpG-ODN(P>0.05).The tyrosinase activity and melanin synthesis were promoted by 10 μg/ml CpG-ODN,whose effects resembled that of 25 nmol/ml α-MSH(P>0.05).In addition,10 μg/ml CpG-ODN increased the level of PGE2 in the culture supernatants,but no statistical significance compared with the control group(P>0.05).It supposed that due to lagging of detection time.The cytodendrites of melan-α cells treated with 10 μg/ml CpG-ODN for 24 h were elongated and enriched,we could see the second and even tertiary dendrites appearing.Conclusions: The optimal concentration of CpG-ODN for stimulating mouse melanocytes is 10 μg/ml,and its effects are in a time-dependent manner.This study is a primary research about CpG-ODN stimulating normal human melanocytes.
Objective: To investigate the effects of bacteria motif,namely CpG oligodeoxynucleotide(CpG-ODN) on morphology and melanogenesis production of and prostaglandin E2(PGE2) in melanocytes.Methods: The cultured B16 melanoma cells and melan-α melanocytes in vitro were treated with the CpG-ODN.The B16 cell viability,tyrosinase activity,melanin content and the level of PGE2 in the culture supernatants was measured by MTT assay,oxidation of L-dopa,NaOH assay and ELISA assay respectively.In addition,the morphological changes of melan-α cells were observed after the CpG-ODN was added.Results: The CpG-ODN at the concentration of 5 μg/ml promoted the proliferation of B16 cells,10 μg/ml with little effects,and 15 μg/ml with depressant effects on the cell proliferation after drugs intervention for 24 h.After 48 h treating,the cell proliferation rates in various groups became stable and were similar to each other,without significant difference between groups at different concentrations of CpG-ODN(P>0.05).The tyrosinase activity and melanin synthesis were promoted by 10 μg/ml CpG-ODN,whose effects resembled that of 25 nmol/ml α-MSH(P>0.05).In addition,10 μg/ml CpG-ODN increased the level of PGE2 in the culture supernatants,but no statistical significance compared with the control group(P>0.05).It supposed that due to lagging of detection time.The cytodendrites of melan-α cells treated with 10 μg/ml CpG-ODN for 24 h were elongated and enriched,we could see the second and even tertiary dendrites appearing.Conclusions: The optimal concentration of CpG-ODN for stimulating mouse melanocytes is 10 μg/ml,and its effects are in a time-dependent manner.This study is a primary research about CpG-ODN stimulating normal human melanocytes.
2008, 33(6): 677-680.
Abstract:
Objective: To observe the effect of TrKA to the growth of breast cancer cells,investigate the molecular mechanism and provide new target of medicine for the treatment of breast cancer.Methods: The expression of TrKA mRNA and protein was detected by Real Time-PCR and Western-blot before and after interference.The proliferated effect of nerve growth factorl(NGF) was tested by MTT and the protein expression of caspase-3 was detected by Western-blot after RNAi.Results: The levels of TrKA mRNA and protein were decreased by 74.7% and 80.5% respectively after RNAi(P< 0.01);NGF could stimulate the proliferation of MCF-7 cells.The OD490 of the group of MCF-7+NGF was higher than that of the group of MCF-7 at each time point(P<0.01).The effect of NGF to the proliferation of cells was inhibited after RNAi.The absorption of the group TrKA-SiRNA and TrKA-SiRNA+NGF were lower than the group MCF-7 at each time(P<0.01);the protein of caspase-3 was activated in the group of TrKA-SiRNA and TrKA-SiRNA+NGF compared with MCF-7.The protein expression of cleaved caspase-3 was increased(P<0.01).Conclusions: Transfection of TrKA gene SiRNA can inhibit cellular proliferation and promote apoptosis.This suggests that the biologic axis of NGF-TrKA plays a siginificant role in the growth of breast cancer and the axis of interference may be a new target for the breast cancer treatment.
Objective: To observe the effect of TrKA to the growth of breast cancer cells,investigate the molecular mechanism and provide new target of medicine for the treatment of breast cancer.Methods: The expression of TrKA mRNA and protein was detected by Real Time-PCR and Western-blot before and after interference.The proliferated effect of nerve growth factorl(NGF) was tested by MTT and the protein expression of caspase-3 was detected by Western-blot after RNAi.Results: The levels of TrKA mRNA and protein were decreased by 74.7% and 80.5% respectively after RNAi(P< 0.01);NGF could stimulate the proliferation of MCF-7 cells.The OD490 of the group of MCF-7+NGF was higher than that of the group of MCF-7 at each time point(P<0.01).The effect of NGF to the proliferation of cells was inhibited after RNAi.The absorption of the group TrKA-SiRNA and TrKA-SiRNA+NGF were lower than the group MCF-7 at each time(P<0.01);the protein of caspase-3 was activated in the group of TrKA-SiRNA and TrKA-SiRNA+NGF compared with MCF-7.The protein expression of cleaved caspase-3 was increased(P<0.01).Conclusions: Transfection of TrKA gene SiRNA can inhibit cellular proliferation and promote apoptosis.This suggests that the biologic axis of NGF-TrKA plays a siginificant role in the growth of breast cancer and the axis of interference may be a new target for the breast cancer treatment.
2008, 33(6): 680-682.
Abstract:
Objective: To explore the identification of the proglottids of Bertiella studeri for providing evidences for the pathogenic diagnosis of human bertiellosis.Methods: The segments were collected from the patient's feces and observed directly' and then fixed with 10% formaline,the morphology data of the proglottids were measured,recorded and analyzed.Results: The segment collected in the patient's feces contained several to more than dozens proglotiids,the average length of the proglottids was 0.1 cm and width 0.68-1.10 cm.The living segment could contract strongly and formed a special biangular end.Conclusions: The shape of the segment collected from the patient's feces could changed remarkably by means of contraction and the morphologic characteristics of the proglottids that the width is far more than its length,both the characteristics and the epidemic data are important clue for bertiellosis diagnosis.
Objective: To explore the identification of the proglottids of Bertiella studeri for providing evidences for the pathogenic diagnosis of human bertiellosis.Methods: The segments were collected from the patient's feces and observed directly' and then fixed with 10% formaline,the morphology data of the proglottids were measured,recorded and analyzed.Results: The segment collected in the patient's feces contained several to more than dozens proglotiids,the average length of the proglottids was 0.1 cm and width 0.68-1.10 cm.The living segment could contract strongly and formed a special biangular end.Conclusions: The shape of the segment collected from the patient's feces could changed remarkably by means of contraction and the morphologic characteristics of the proglottids that the width is far more than its length,both the characteristics and the epidemic data are important clue for bertiellosis diagnosis.
2008, 33(6): 683-686.
Abstract:
Objective: To explore the relationship of construction practice bases for preventive medicine specialty with graduates employment.Methods: According to the necessity of preventive medicine specialty students practice and employment,the specialty practice bases was builed in the developed area of Jiangsu,Zhejiang and Shanghai(Jiang,Zhe,Hu) for graduates employment to create favourabale conditions.Results: The 14 practice bases(deal with 40 practice units) of preventive medicine specialty was established in the Shanghai,Jiangsu,et al five area(including central of disease preventive and control,sanition supervise bureau or institute and clinical practice hospital).The graduates employment rate was all 100% for three years.The graduates employment rate in out Anhui privince is going up from 28.1% of 2005 year first graduates to 61.1% of 2007 year graduates,but over 93% of graduates employment was in the Jiang,Zhe,Hu and priphery area.Conclusions: The practice was proved that the specialty practice was an important link for undergraduates education.Not only the strenghening practice bases construction can ensure to achieve specialty education goal and education quality,but also practice bases construction was to strenghen general ability of specialty students and to raise employment rate of specialty graduates.
Objective: To explore the relationship of construction practice bases for preventive medicine specialty with graduates employment.Methods: According to the necessity of preventive medicine specialty students practice and employment,the specialty practice bases was builed in the developed area of Jiangsu,Zhejiang and Shanghai(Jiang,Zhe,Hu) for graduates employment to create favourabale conditions.Results: The 14 practice bases(deal with 40 practice units) of preventive medicine specialty was established in the Shanghai,Jiangsu,et al five area(including central of disease preventive and control,sanition supervise bureau or institute and clinical practice hospital).The graduates employment rate was all 100% for three years.The graduates employment rate in out Anhui privince is going up from 28.1% of 2005 year first graduates to 61.1% of 2007 year graduates,but over 93% of graduates employment was in the Jiang,Zhe,Hu and priphery area.Conclusions: The practice was proved that the specialty practice was an important link for undergraduates education.Not only the strenghening practice bases construction can ensure to achieve specialty education goal and education quality,but also practice bases construction was to strenghen general ability of specialty students and to raise employment rate of specialty graduates.
2008, 33(6): 712-715.
Abstract:
Objective: To investigate a new methods in the location of cerebral sulcus.Methods: Techniques of configuration tracing based on continuous sections was used to identify and localize precisely the cerebral central sulcus on the continuous transverse MR imaging by using a computer.Results: The cerebral central sulcus was identified and localized precisely on the continuous transverse MR imaging.The results were confirmed by compasion of specimens of sectional brain.Conclusions: The methods of continuous configuration tracing can be used in the study of sectional-imaging anatomy.
Objective: To investigate a new methods in the location of cerebral sulcus.Methods: Techniques of configuration tracing based on continuous sections was used to identify and localize precisely the cerebral central sulcus on the continuous transverse MR imaging by using a computer.Results: The cerebral central sulcus was identified and localized precisely on the continuous transverse MR imaging.The results were confirmed by compasion of specimens of sectional brain.Conclusions: The methods of continuous configuration tracing can be used in the study of sectional-imaging anatomy.
2008, 33(6): 716-719.
Abstract:
Objective: To investigate a new method for the study on coronal and sagittal sectional-imaging anatomy.Methods: Techniques of three dimension cursor based on volume multi-orientation sections were used to identify and localize precisely the cerebral central sulcus on the continuous coronal and sagittal MRI by using a person computer.Results: The cerebral central sulcus on the continuous coronal and sagittal MRI were identified and localized precisely.The results were unanimous with the continuous tracing configuration.Conclusions: The method of 3-Dimension-Cursor can be used in the study of sectional-imaging anatomy.
Objective: To investigate a new method for the study on coronal and sagittal sectional-imaging anatomy.Methods: Techniques of three dimension cursor based on volume multi-orientation sections were used to identify and localize precisely the cerebral central sulcus on the continuous coronal and sagittal MRI by using a person computer.Results: The cerebral central sulcus on the continuous coronal and sagittal MRI were identified and localized precisely.The results were unanimous with the continuous tracing configuration.Conclusions: The method of 3-Dimension-Cursor can be used in the study of sectional-imaging anatomy.
2008, 33(6): 719-722.
Abstract:
Objective: To reconstruct and visualize three-dimentional model of Rolando fissure for the stereotactic anatomy.Methods: Rolando fissure,the lateral ventricle,the cerebrum and the borderyline of head were identified and segmented on a series of transverse MRI of head,and the three-dimentional model of them were reconstructed in 3D-Doctor workstation with the method of surface rendering.Results: The three-dimentional model of Rolando fissure,other interesting structures and their spatial relation were observated in three-dimensional virtual space in random angles.Conclusions: Anatomical observation and measurement can be implemented in the three-dimentional model.Furthermore,it was hoped that the model would benefit to virtual surgical training and surgical planning in neurosurgery.
Objective: To reconstruct and visualize three-dimentional model of Rolando fissure for the stereotactic anatomy.Methods: Rolando fissure,the lateral ventricle,the cerebrum and the borderyline of head were identified and segmented on a series of transverse MRI of head,and the three-dimentional model of them were reconstructed in 3D-Doctor workstation with the method of surface rendering.Results: The three-dimentional model of Rolando fissure,other interesting structures and their spatial relation were observated in three-dimensional virtual space in random angles.Conclusions: Anatomical observation and measurement can be implemented in the three-dimentional model.Furthermore,it was hoped that the model would benefit to virtual surgical training and surgical planning in neurosurgery.
2008, 33(6): 723-725.
Abstract:
Objective: To observe the presence of the paracingulate sulcus(PCS) and the continuity of the cingulate sulcus(CS).Methods: Thirty healthy adult brain sagittal-sectional MRI data were imported into efilm workstation in the form of Dicom 3.0,and the lateral median sagittal planes of every two hemispheres were selected.The presence of the PCS and the continuity of the CS were observed.Results: The presence of the PCS:the left hemisphere was 56.67%,the right was 26.67%.The presence of continuous cingulate sulcus:the left was 63.33% when no PCS,and 88.24% when there was PCS;The right was 50% when no PCS,and 71.43% when there was PCS.Conclusions: The results reveal a striking hemispheric asymmetry in the presence of paracingulate sulcus and considerable variability in the above morphological features.
Objective: To observe the presence of the paracingulate sulcus(PCS) and the continuity of the cingulate sulcus(CS).Methods: Thirty healthy adult brain sagittal-sectional MRI data were imported into efilm workstation in the form of Dicom 3.0,and the lateral median sagittal planes of every two hemispheres were selected.The presence of the PCS and the continuity of the CS were observed.Results: The presence of the PCS:the left hemisphere was 56.67%,the right was 26.67%.The presence of continuous cingulate sulcus:the left was 63.33% when no PCS,and 88.24% when there was PCS;The right was 50% when no PCS,and 71.43% when there was PCS.Conclusions: The results reveal a striking hemispheric asymmetry in the presence of paracingulate sulcus and considerable variability in the above morphological features.
2008, 33(6): 725-727.
Abstract:
Objective: To explore the morphological characteristic of cerebral parietooccipital sulcus on series axial MR imaging of brain.Methods: On MRI of 30 normal volunteers' brain,parietooccipital sulcus was observed by the method of continuous tracking and three dimensional cursor on eFilm 2.1 workstation.The parietooccipital sulcus was identified and observated,and the morphological characteristic was analyzed on series sections.Results: The parietooccipital sulcus showed shaped-"一","U" and "Y" on cross-sectional MR imaging(Z=-6-36mm).The sulcus appeared "一"-shaped most commonly(87.3%) on 622 sections,and the others appeared U-shaped and Y-shaped(12.7%).Conclusions: The parietooccipital sulcus is identified accurately,and the cuneus and precuneus can be localized easily depend on the results.
Objective: To explore the morphological characteristic of cerebral parietooccipital sulcus on series axial MR imaging of brain.Methods: On MRI of 30 normal volunteers' brain,parietooccipital sulcus was observed by the method of continuous tracking and three dimensional cursor on eFilm 2.1 workstation.The parietooccipital sulcus was identified and observated,and the morphological characteristic was analyzed on series sections.Results: The parietooccipital sulcus showed shaped-"一","U" and "Y" on cross-sectional MR imaging(Z=-6-36mm).The sulcus appeared "一"-shaped most commonly(87.3%) on 622 sections,and the others appeared U-shaped and Y-shaped(12.7%).Conclusions: The parietooccipital sulcus is identified accurately,and the cuneus and precuneus can be localized easily depend on the results.
2008, 33(6): 728-731.
Abstract:
Objective: To explore the effect of As2O3 on expressions of NF-κB,C-IAP2 and caspase-3 in melanoma A375 cell line.Methods: The expression of NF-κB p65 nuclear protein and the activity of caspase-3 were examined by Western blot,the expression of C-IAP2 mRNA was examined by semi-quantitative RT-PCR in A375 cells.Results: Treated with 2.5,5.0 and 10.0 μmol/L As2O3 for 24 h,the expression ratio of NF-κB p65 nucleoprotein was(39.60±7.76)%,(10.17±0.90)% and(4.43±0.91)%,respectively;the expression ratio of cleaved caspase-3 was(10.03±2.06)%,(23.43±3.28)% and(35.70±4.61)%,respectively;the ratio of C-IAP2 mRNA was(66.78±15.46)%,(30.16±5.52)% and(6.46±4.54)%,respectively.There was significant difference between control group and experiment group(P<0.05 to P<0.01) (P<0.01).With the concentration of As2O3 increasing,caspase-3 was activated dramatically,NF-κB p65 and C-IAP2 mRNA decreased dramatically.Conclusions: The signal transduction pathway of NF-κB,C-IAP2 and caspase-3 may be associated the apoptosis of A375 cells induced by As2O3.
Objective: To explore the effect of As2O3 on expressions of NF-κB,C-IAP2 and caspase-3 in melanoma A375 cell line.Methods: The expression of NF-κB p65 nuclear protein and the activity of caspase-3 were examined by Western blot,the expression of C-IAP2 mRNA was examined by semi-quantitative RT-PCR in A375 cells.Results: Treated with 2.5,5.0 and 10.0 μmol/L As2O3 for 24 h,the expression ratio of NF-κB p65 nucleoprotein was(39.60±7.76)%,(10.17±0.90)% and(4.43±0.91)%,respectively;the expression ratio of cleaved caspase-3 was(10.03±2.06)%,(23.43±3.28)% and(35.70±4.61)%,respectively;the ratio of C-IAP2 mRNA was(66.78±15.46)%,(30.16±5.52)% and(6.46±4.54)%,respectively.There was significant difference between control group and experiment group(P<0.05 to P<0.01) (P<0.01).With the concentration of As2O3 increasing,caspase-3 was activated dramatically,NF-κB p65 and C-IAP2 mRNA decreased dramatically.Conclusions: The signal transduction pathway of NF-κB,C-IAP2 and caspase-3 may be associated the apoptosis of A375 cells induced by As2O3.
2008, 33(6): 731-735.
Abstract:
Objective: To study the relation between the change of ribonuclease inhibitor(RI) and malignant proferations in different malignant degree human breast cancer cell lines MCF-7、MDA-MB-231、SKBR-3.Methods: Cell profigeration was determined by MTT assay;The cell cycle was measured by flow cytometry(FCM);the expression of RI mRNA was examined by semi-quantitative RT-PCR;immunohistochemistry was used to detected the protein expression of RI.Results: Human breast cancer cell lines MCF-7 cell malignant proliferation.S period was less than the propotion of human breast cancer cell lines MDA-MB-231,SKBR-3(P<0.05-P<0.01),and the expressions of RI mRNA and protein in the human breast cancer cell lines MCF-7 were higer than that of human breast cancer cell lines MDA-MB-231、SKBR-3(P<0.01).Conclusions: RI and human breast cancer cells malignant profigeration growth has certain relevance.
Objective: To study the relation between the change of ribonuclease inhibitor(RI) and malignant proferations in different malignant degree human breast cancer cell lines MCF-7、MDA-MB-231、SKBR-3.Methods: Cell profigeration was determined by MTT assay;The cell cycle was measured by flow cytometry(FCM);the expression of RI mRNA was examined by semi-quantitative RT-PCR;immunohistochemistry was used to detected the protein expression of RI.Results: Human breast cancer cell lines MCF-7 cell malignant proliferation.S period was less than the propotion of human breast cancer cell lines MDA-MB-231,SKBR-3(P<0.05-P<0.01),and the expressions of RI mRNA and protein in the human breast cancer cell lines MCF-7 were higer than that of human breast cancer cell lines MDA-MB-231、SKBR-3(P<0.01).Conclusions: RI and human breast cancer cells malignant profigeration growth has certain relevance.
2008, 33(6): 736-739.
Abstract:
Objective: To explore proliferative activity of CD4+CD25+ Treg cells induced by transforming growth factor-β1(TGF-β1) in neonatal cord blood.Methods: Cord blood mononuclear cells were labeled with carboxyfluorescein diacetate,succinimidyl ester(CFSE),and then stimulated with TGF-β1,CD3mAb and cultured in 10% NBS-1640 medium in presence of IL-2.The dynamics of proliferation of CD4+CD25+ and CD8+ T cells on day 4,day 6,day 8 after culture was determined by flow cytometry.Results: Proliferative kinetics analysis showed proliferation index(PI) on day 6 and day 8 of culture with TGF-β1 in CD4+CD25+ Treg cells(11.52 and 23.04) were significantly higher than those in control cells(6.68 and 10.46) and those cultured with TGF-β1 in CD8+ T cells(4.23 and 5.43).On day 8 of culture with TGF-β1,proportion of the generations from 8th to 10th in CD4+CD25+ Treg(52.74%) was strikingly higher than those in control cells(36.26%) and those in CD8+ T cells cultured with TGF-β1(13.54%),while the proportion of the generations from 8th to 10th in control CD8+ T cells was 29.44%.Conclusions: Proliferative activity of CD4+CD25+ Treg cells induced by TGF-β1 in neonatal cord blood was obviously stronger than that of CD8+ T cells.
Objective: To explore proliferative activity of CD4+CD25+ Treg cells induced by transforming growth factor-β1(TGF-β1) in neonatal cord blood.Methods: Cord blood mononuclear cells were labeled with carboxyfluorescein diacetate,succinimidyl ester(CFSE),and then stimulated with TGF-β1,CD3mAb and cultured in 10% NBS-1640 medium in presence of IL-2.The dynamics of proliferation of CD4+CD25+ and CD8+ T cells on day 4,day 6,day 8 after culture was determined by flow cytometry.Results: Proliferative kinetics analysis showed proliferation index(PI) on day 6 and day 8 of culture with TGF-β1 in CD4+CD25+ Treg cells(11.52 and 23.04) were significantly higher than those in control cells(6.68 and 10.46) and those cultured with TGF-β1 in CD8+ T cells(4.23 and 5.43).On day 8 of culture with TGF-β1,proportion of the generations from 8th to 10th in CD4+CD25+ Treg(52.74%) was strikingly higher than those in control cells(36.26%) and those in CD8+ T cells cultured with TGF-β1(13.54%),while the proportion of the generations from 8th to 10th in control CD8+ T cells was 29.44%.Conclusions: Proliferative activity of CD4+CD25+ Treg cells induced by TGF-β1 in neonatal cord blood was obviously stronger than that of CD8+ T cells.
2008, 33(6): 739-742.
Abstract:
Objective: To explore relationship between polymorphism of the human intestinal fatty acid binding protein gene and metabolic parameters of type 2 diabetic patients.Methods: One hundred and fourty Han nationality in Bengbu were screened for presence of codon 54 variation by PCR-RFLP assay,identified by the enzyme HhaⅠ[Eighty patients with type 2 diabetes mellitus(T2DM) and sixty normal controls(NC)].Results: The polymorphic restriction site was found in the FABP 2 identified by the enzyme HhaⅠin Han nationality of Bengbu(the freqencies of Ala54 and thr54 in Bengbu were 0.67and 0.33,respectively).The type 2 diabetes-mellitus(T2DM) subjects had higher HOMR-IR,FINS,FPG,TC,TG,LDL and lower ISI than NC subjects.The 2DM subjects with genotype Thr54(+) (Thr54 homozygotes and heterozygotes) had higher FPG、FINS、HOMR-IR、TG、LDL(P<0.01) and lower ISI(P<0.01) than those with genotype Thr(-) (Ala54 homozygotes).Conclusions: The polymorphy of FABP2 has been identified in the Han nationality in Bengbu.The genotype Thr54 homozygotes and heterozygotes of FABP2 gene seems to be associated with the insulin resistance of the type 2 diabetic patients.They also may be involved in the determination of lipoprotein profiles in the type 2 diabetic patients.
Objective: To explore relationship between polymorphism of the human intestinal fatty acid binding protein gene and metabolic parameters of type 2 diabetic patients.Methods: One hundred and fourty Han nationality in Bengbu were screened for presence of codon 54 variation by PCR-RFLP assay,identified by the enzyme HhaⅠ[Eighty patients with type 2 diabetes mellitus(T2DM) and sixty normal controls(NC)].Results: The polymorphic restriction site was found in the FABP 2 identified by the enzyme HhaⅠin Han nationality of Bengbu(the freqencies of Ala54 and thr54 in Bengbu were 0.67and 0.33,respectively).The type 2 diabetes-mellitus(T2DM) subjects had higher HOMR-IR,FINS,FPG,TC,TG,LDL and lower ISI than NC subjects.The 2DM subjects with genotype Thr54(+) (Thr54 homozygotes and heterozygotes) had higher FPG、FINS、HOMR-IR、TG、LDL(P<0.01) and lower ISI(P<0.01) than those with genotype Thr(-) (Ala54 homozygotes).Conclusions: The polymorphy of FABP2 has been identified in the Han nationality in Bengbu.The genotype Thr54 homozygotes and heterozygotes of FABP2 gene seems to be associated with the insulin resistance of the type 2 diabetic patients.They also may be involved in the determination of lipoprotein profiles in the type 2 diabetic patients.
2008, 33(6): 743-746.
Abstract:
Objective: To investigate the function of CD4+CD25+ T regulatory(CD4+CD25+ Treg) cells in NOD mice.Methods: The frequency of CD4+CD25+ T cells from pancreatic lympho node(PLN) was analysed,the percentages change over time in the proportion of NOD CD4+ CD25+ T cells expressing Foxp3 were observed,mean fluorescent intensity(MFI) of Foxp3 in CD4+ CD25+ FoxP3+ T cells was assayed,and in vitro suppression assay was performed.Results: No percentages change over time in the proportion of NOD CD4+ CD25+ T cells expressing Foxp3 were observed.But the mean fluorescent intensity(MFI) of Foxp3 in CD4+CD25+FoxP3+ T cells decreased with time.In vitro suppression assay,PLN CD4+CD25+ T cells show decrease in their ability to suppress the proliferation of CD4+CD25- T cells.There exists some relationship between the impairment of in vitro suppression and the reduction at the protein expression levels of Foxp3 on a per-cell basis.Conclusions: It is the impairment of the function of CD4+CD25+ Treg cells that results in the development of diabetes mellitus in NOD mice.
Objective: To investigate the function of CD4+CD25+ T regulatory(CD4+CD25+ Treg) cells in NOD mice.Methods: The frequency of CD4+CD25+ T cells from pancreatic lympho node(PLN) was analysed,the percentages change over time in the proportion of NOD CD4+ CD25+ T cells expressing Foxp3 were observed,mean fluorescent intensity(MFI) of Foxp3 in CD4+ CD25+ FoxP3+ T cells was assayed,and in vitro suppression assay was performed.Results: No percentages change over time in the proportion of NOD CD4+ CD25+ T cells expressing Foxp3 were observed.But the mean fluorescent intensity(MFI) of Foxp3 in CD4+CD25+FoxP3+ T cells decreased with time.In vitro suppression assay,PLN CD4+CD25+ T cells show decrease in their ability to suppress the proliferation of CD4+CD25- T cells.There exists some relationship between the impairment of in vitro suppression and the reduction at the protein expression levels of Foxp3 on a per-cell basis.Conclusions: It is the impairment of the function of CD4+CD25+ Treg cells that results in the development of diabetes mellitus in NOD mice.
2008, 33(6): 747-749.
Abstract:
Objective: To determine the antibiotic concentration in disc space under different micro-circumstances and to provide theoretic basis for clinical antibiotic treatment of pyogenic discitis.Methods: The pyogenic discitis model was established by injecting Staphylococcus aureus into the intervertebral space of the dog.Cefazolin was injected into the inferior vena cava,and 30 minutes later,the infected disc(B) was obtained and the infected disc debrided.One week later,cefazolin 40 mg/kg was injected and the tissues of infected disc space was harvested after debridement(D) and normal nucleus(E).The cefazolin concentrations of B,D,and E were tested by High-pressure Liquid Chromatography method.Results: The cefazolin concentrations of the normal nucleus,infected disc and the tissues of the infected disc space after debridement were(4.62±1.74) μg/mg,(7.00±2.48) μg/mg,and(14.11±2.10) μg/mg,respectively.Significant difference in cefazolin concentration existed between the tissues of infected disc space after debridement and the normal nucleus,as well as between the tissues of infected disc space after debridement and the infected disc(P<0.01).Conclusions: The concentration of cefazolin in the tissues of infected disc space rises significantly after debridement.
Objective: To determine the antibiotic concentration in disc space under different micro-circumstances and to provide theoretic basis for clinical antibiotic treatment of pyogenic discitis.Methods: The pyogenic discitis model was established by injecting Staphylococcus aureus into the intervertebral space of the dog.Cefazolin was injected into the inferior vena cava,and 30 minutes later,the infected disc(B) was obtained and the infected disc debrided.One week later,cefazolin 40 mg/kg was injected and the tissues of infected disc space was harvested after debridement(D) and normal nucleus(E).The cefazolin concentrations of B,D,and E were tested by High-pressure Liquid Chromatography method.Results: The cefazolin concentrations of the normal nucleus,infected disc and the tissues of the infected disc space after debridement were(4.62±1.74) μg/mg,(7.00±2.48) μg/mg,and(14.11±2.10) μg/mg,respectively.Significant difference in cefazolin concentration existed between the tissues of infected disc space after debridement and the normal nucleus,as well as between the tissues of infected disc space after debridement and the infected disc(P<0.01).Conclusions: The concentration of cefazolin in the tissues of infected disc space rises significantly after debridement.
2008, 33(6): 749-750.
Abstract:
Objective: To study the CT features of cystic renal cell carcinoma in order to improve accuracy of the preoperatiue diagnosis.Methods: The CT imaging data of 11 cases of cystic renal cell carcinoma confirmed by pathology were retrospectively analyzed.Results: Seven cases demonstrated multilocular cystic mass with irregular inner wall and septas,and the inner wall and septa were obviously enhanced.Four cases revealed unilocular cystic renal cell carcinoma with enhanced mural nodules and non-enhanced muddy cystic content.Conclusions: The cystic renal cell carcinoma has certain CT characteristics,which is of value for surgical operation.
Objective: To study the CT features of cystic renal cell carcinoma in order to improve accuracy of the preoperatiue diagnosis.Methods: The CT imaging data of 11 cases of cystic renal cell carcinoma confirmed by pathology were retrospectively analyzed.Results: Seven cases demonstrated multilocular cystic mass with irregular inner wall and septas,and the inner wall and septa were obviously enhanced.Four cases revealed unilocular cystic renal cell carcinoma with enhanced mural nodules and non-enhanced muddy cystic content.Conclusions: The cystic renal cell carcinoma has certain CT characteristics,which is of value for surgical operation.
2008, 33(6): 751-753.
Abstract:
Objective: To investigate the hemodynamic effects of aerosolized iloprost on pulmonary hypertension(PAH) of patients with congenital heart disease(CHD).Methods: Before and after inhaled iloprost hemodynamic parameters was measure in 20 PAH patients with CHD.Results: Iloprost inhalation can decreased systolic pulmonary artery pressure,diastolic pulmonary artery pressure(PAP),mean pulmonary artery pressure,and pulmonary vascular resistance(PVR).Iloprost inhalation increased systemic arterial oxygen saturation,systemic arterial partial pressure of oxygen,systemic blood flow,pulmonary blood flow and cardiac index(CI).And there was no significant influence on mixed venous oxygen content,mixed venous oxygen saturation,systolic arterial pressure,diastolic arterial pressure,mean systemic arterial pressure,pulmonary artery wedge pressure and heart rate.Conclusions: Aerosolized iloprost can safely and effectively decrease PAP,PVR and increase CI.
Objective: To investigate the hemodynamic effects of aerosolized iloprost on pulmonary hypertension(PAH) of patients with congenital heart disease(CHD).Methods: Before and after inhaled iloprost hemodynamic parameters was measure in 20 PAH patients with CHD.Results: Iloprost inhalation can decreased systolic pulmonary artery pressure,diastolic pulmonary artery pressure(PAP),mean pulmonary artery pressure,and pulmonary vascular resistance(PVR).Iloprost inhalation increased systemic arterial oxygen saturation,systemic arterial partial pressure of oxygen,systemic blood flow,pulmonary blood flow and cardiac index(CI).And there was no significant influence on mixed venous oxygen content,mixed venous oxygen saturation,systolic arterial pressure,diastolic arterial pressure,mean systemic arterial pressure,pulmonary artery wedge pressure and heart rate.Conclusions: Aerosolized iloprost can safely and effectively decrease PAP,PVR and increase CI.
2008, 33(6): 754-756.
Abstract:
Objective: To study the expressions of Cyclin D1 and p16 in the tissues of non-small-cell lung cancer(NSCLC) and its clinical significance.Methods: The expressions of Cyclin D1 and p16 in 62 cases of primary lung cancer were determined by immunohistochemical method and 20 cases of pulmonary benign cases acted as control.Results: The expressions of Cyclin D1 and p16 were high in the tissues of lung cancer,with a positive rate of 62.9% and 56.5%,respectively.The positive rates of Cyclin D1 and p16 in the 20 controls was 85.0%.The expression of Cyclin D1 was not related to the tumor's diameter,lymph node metastasis or clinical stages(P>0.05).The level of p16 was not related to the size of the tumor,but was associated with the lymph node metastases and clinical stages(P<0.005).Conclusions: Over expression of Cyclin D1 is related to the carcinogenesis and may act as a marker in the early diagnosis of lung cancer,but it has no correlation with the biological behavior or prognosis of lung cancer,while p16 is associated with the biological behavior and prognosis of lung cancer.
Objective: To study the expressions of Cyclin D1 and p16 in the tissues of non-small-cell lung cancer(NSCLC) and its clinical significance.Methods: The expressions of Cyclin D1 and p16 in 62 cases of primary lung cancer were determined by immunohistochemical method and 20 cases of pulmonary benign cases acted as control.Results: The expressions of Cyclin D1 and p16 were high in the tissues of lung cancer,with a positive rate of 62.9% and 56.5%,respectively.The positive rates of Cyclin D1 and p16 in the 20 controls was 85.0%.The expression of Cyclin D1 was not related to the tumor's diameter,lymph node metastasis or clinical stages(P>0.05).The level of p16 was not related to the size of the tumor,but was associated with the lymph node metastases and clinical stages(P<0.005).Conclusions: Over expression of Cyclin D1 is related to the carcinogenesis and may act as a marker in the early diagnosis of lung cancer,but it has no correlation with the biological behavior or prognosis of lung cancer,while p16 is associated with the biological behavior and prognosis of lung cancer.
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