Author Login
Experts Review
Academic Editor
Editor-in-chief
2011 Vol. 36, No. 10
Display Method:
2011, 36(10): 1041-1042,1046.
Abstract:
Objective: To explore the prompt methods in determination of viability of the adult of Trichinella spiralis. Methods: The adult worms of Trichinella spiralis were stained with 0.5% safranine,1.0% neutral red or 0.5% methylene blue. Results: The dead adults were observed when stained with 0.5% safranine or 1.0% neutral red in the study. The vital adults were unstained with all three stains. Conclusions: Both 0.5% safranine staining and 1.0% neutral red staining are prompt method in determination of viability of the adult of Trichinella spiralis.
Objective: To explore the prompt methods in determination of viability of the adult of Trichinella spiralis. Methods: The adult worms of Trichinella spiralis were stained with 0.5% safranine,1.0% neutral red or 0.5% methylene blue. Results: The dead adults were observed when stained with 0.5% safranine or 1.0% neutral red in the study. The vital adults were unstained with all three stains. Conclusions: Both 0.5% safranine staining and 1.0% neutral red staining are prompt method in determination of viability of the adult of Trichinella spiralis.
2011, 36(10): 1043-1046.
Abstract:
Objective: To measure the changes of diaphragm contracting function and ultrastructure in diet-induced obesity impuberism female Sprague-Dawley rats,and explore the possible mechanism. Methods: Thirty female Sprague-Dawley weaning rats were randomly divided into model group and control group according to their weight. The rats in control group were fed with standard chow and in model group were fed with high fat diet for 8 weeks. Body weight was compared between the both groups at the end of experiment. In perfusion diaphragm strips model in vitro,the parameters which reflex the diaphragm contractility including peak twitch tension (Pt),maximum tetanic tension (Po),force-frequency relationship and fatigue index (FI) were assessed by Medlab-U/4C biological signal collecting system. The changes of diaphragm ultrastructure were studied by electron microscopy in rats. Results: Compared with the rats in control group,Pt,Po,FI were lower in model group (P < 0.05-P < 0.01). Compared with the rats in control group,tetanic force under the stimulus frequency of 10,20,40,60,100 Hz were decreased in model group (P < 0.01). Electron microscope morphometry of diaphragm in model group revealed a large number of lipid droplets was distributed in diaphragm,muscle fiber edema,and small number of filaments disorder were observed;the quantity of mitochondria was decreased,mitochondria was oedema and expanded,its cristae broken and vague,a great quantity of mitochondria were vacuolization or vesiculation,and myelin figures was observed. Conclusions: In dietinduced obesity Sprague-Dawley rats,the contractile function of diaphragm is decreased and likely to fatigue. The mechanism may be related to fat deposits in diaphragm and breakage of mitochondria.
Objective: To measure the changes of diaphragm contracting function and ultrastructure in diet-induced obesity impuberism female Sprague-Dawley rats,and explore the possible mechanism. Methods: Thirty female Sprague-Dawley weaning rats were randomly divided into model group and control group according to their weight. The rats in control group were fed with standard chow and in model group were fed with high fat diet for 8 weeks. Body weight was compared between the both groups at the end of experiment. In perfusion diaphragm strips model in vitro,the parameters which reflex the diaphragm contractility including peak twitch tension (Pt),maximum tetanic tension (Po),force-frequency relationship and fatigue index (FI) were assessed by Medlab-U/4C biological signal collecting system. The changes of diaphragm ultrastructure were studied by electron microscopy in rats. Results: Compared with the rats in control group,Pt,Po,FI were lower in model group (P < 0.05-P < 0.01). Compared with the rats in control group,tetanic force under the stimulus frequency of 10,20,40,60,100 Hz were decreased in model group (P < 0.01). Electron microscope morphometry of diaphragm in model group revealed a large number of lipid droplets was distributed in diaphragm,muscle fiber edema,and small number of filaments disorder were observed;the quantity of mitochondria was decreased,mitochondria was oedema and expanded,its cristae broken and vague,a great quantity of mitochondria were vacuolization or vesiculation,and myelin figures was observed. Conclusions: In dietinduced obesity Sprague-Dawley rats,the contractile function of diaphragm is decreased and likely to fatigue. The mechanism may be related to fat deposits in diaphragm and breakage of mitochondria.
2011, 36(10): 1047-1049.
Abstract:
Objective: To construct the wild-type and mutant human apolipoprotein M (ApoM) expression plasmid. Methods: The total RNA was extracted from HepG2 cells with Trizol. ApoM gene was amplified by RT-PCR,and inserted into PMD18-T vectors. After ApoM vector was transformed into E. coli JM109,the positive clones were obtained. Then the recombinant plasmids were identified by restriction enzyme digestion and DNA sequencing. In vitro site-directed mutagenesis was carried out with site-directed mutagenesis kit. Successful site-directed mutagenesis was conformed by DNA sequencing. The wild-type and mutant coding genes were subcloned into prokaryotic expression vector pGEX-KG and eukaryotic expression vector pcDNA3.1(+). The recombinant plasmids were transformed into E. coli DH5α and identified by restriction endonuclease digestion and DNA sequencing. Results: The human ApoM gene was successfully cloned. The wild-type and mutant pGEX-KG-ApoM and pcDNA3.1(+)-ApoM recombinant plasmids were constructed successfully. Conclusions: With the techniques of RT-PCR,sited-directed mutagenesis and gene recombination,the wild-type and mutant pGEX-KG-ApoM and pcDNA3.1(+)-ApoM were successfully constructed,which lays the foundation for further investigating the role of ApoM.
Objective: To construct the wild-type and mutant human apolipoprotein M (ApoM) expression plasmid. Methods: The total RNA was extracted from HepG2 cells with Trizol. ApoM gene was amplified by RT-PCR,and inserted into PMD18-T vectors. After ApoM vector was transformed into E. coli JM109,the positive clones were obtained. Then the recombinant plasmids were identified by restriction enzyme digestion and DNA sequencing. In vitro site-directed mutagenesis was carried out with site-directed mutagenesis kit. Successful site-directed mutagenesis was conformed by DNA sequencing. The wild-type and mutant coding genes were subcloned into prokaryotic expression vector pGEX-KG and eukaryotic expression vector pcDNA3.1(+). The recombinant plasmids were transformed into E. coli DH5α and identified by restriction endonuclease digestion and DNA sequencing. Results: The human ApoM gene was successfully cloned. The wild-type and mutant pGEX-KG-ApoM and pcDNA3.1(+)-ApoM recombinant plasmids were constructed successfully. Conclusions: With the techniques of RT-PCR,sited-directed mutagenesis and gene recombination,the wild-type and mutant pGEX-KG-ApoM and pcDNA3.1(+)-ApoM were successfully constructed,which lays the foundation for further investigating the role of ApoM.
2011, 36(10): 1050-1053,1057.
Abstract:
Objective: To set up the retinoic acid (RA)-induced exencephaly model in KM mice,and analyze the toxicity of RA to fetus and its mother. Methods: The KM mice were randomly divided into test and control groups. The mice were orally treated with a single dose of 20,30,40 and 50 mg/kg of all-trans-RA which dissolved in peanut oil at a selective time of gestational day (GD)7.50 d,GD7.75 d,GD8.00 d or GD8.50 d. The mice in control group were administrated with a volume of peanut oil,equal to the dose of RA. Results: RA could lead to exposed exencephaly at different development stage and different drug dosage,and the incidence of exencephaly was increased with the increasing of RA dosage and decreased with the addtion of the gestational age. The ideal animal model of exencephaly was established by administrating 30 mg/kg RA to GD 7.75 d mice. In addition,the fetus in test group was displayed an open neural tube and the GD16.50 d fetus was showed a exposed brain tissue at cephalosume. The fetal cranial bone and skin were incomplete developed. Conclusions: The mouse model of exencephaly was sucessfully established and laid the basis for the further study.
Objective: To set up the retinoic acid (RA)-induced exencephaly model in KM mice,and analyze the toxicity of RA to fetus and its mother. Methods: The KM mice were randomly divided into test and control groups. The mice were orally treated with a single dose of 20,30,40 and 50 mg/kg of all-trans-RA which dissolved in peanut oil at a selective time of gestational day (GD)7.50 d,GD7.75 d,GD8.00 d or GD8.50 d. The mice in control group were administrated with a volume of peanut oil,equal to the dose of RA. Results: RA could lead to exposed exencephaly at different development stage and different drug dosage,and the incidence of exencephaly was increased with the increasing of RA dosage and decreased with the addtion of the gestational age. The ideal animal model of exencephaly was established by administrating 30 mg/kg RA to GD 7.75 d mice. In addition,the fetus in test group was displayed an open neural tube and the GD16.50 d fetus was showed a exposed brain tissue at cephalosume. The fetal cranial bone and skin were incomplete developed. Conclusions: The mouse model of exencephaly was sucessfully established and laid the basis for the further study.
2011, 36(10): 1054-1057.
Abstract:
Objective: To investigate the effect of Ara-C at low concentrations on extracellular matrix metalloproteinase inducer (CD147) expression in Jurkat cells. Methods: Jurkat cells were divided into six groups:control group and cells treated with different concentrations of Ara-C at 1,5,10,20,and 30 ng/ml. CD147 mRNA and protein expression were assayed by RT-PCR,Western blot and flow cytometry,respectively. The colony formation in Jurkat cells which was stimulated with Ara-C or not was observed by inverted microscope. Results: Different concentrations of Ara-C significantly up-regulated the expressions of CD147 mRNA and protein,and increased the colony formation in Jurkat cells (P < 0.01). Conclusions: Ara-C at low concentrations can enhance the expression of CD147 and increase the adhesive force in Jurkat cells.
Objective: To investigate the effect of Ara-C at low concentrations on extracellular matrix metalloproteinase inducer (CD147) expression in Jurkat cells. Methods: Jurkat cells were divided into six groups:control group and cells treated with different concentrations of Ara-C at 1,5,10,20,and 30 ng/ml. CD147 mRNA and protein expression were assayed by RT-PCR,Western blot and flow cytometry,respectively. The colony formation in Jurkat cells which was stimulated with Ara-C or not was observed by inverted microscope. Results: Different concentrations of Ara-C significantly up-regulated the expressions of CD147 mRNA and protein,and increased the colony formation in Jurkat cells (P < 0.01). Conclusions: Ara-C at low concentrations can enhance the expression of CD147 and increase the adhesive force in Jurkat cells.
2011, 36(10): 1058-1060,1063.
Abstract:
Objective: To explore the expression of KAI1/CD82 and C-erbB-2 in breast cancer and its clinieal significance. Methods: The expression of KAI1/CD82,C-erbB-2 and estrogen receptor (ER) in 57 cases of breast cancer and 11 cases of benign mammary lesions were detected by immunohistochemistry S-P method,and their correlation with the clinicopathologic features was analyzed. Results: The positive rate of KAI1/CD82 was 31.58% in the breast cancer and 81.82% in the benign mammary lesions. The positive rate of C-erbB-2 was 71.93% in the breast cancer and zero in benign mammary lesions. The difference was statistically significant between the two groups (P < 0.01). The positive expression of KAI1/CD82 had statistical difference in cases with no metastasis of auxiliary lymph nodes and cases with negative expression of ER (P < 0.05). The positive expression of C-erbB-2 in cases with positive expression of ER was lower than that in cases with negative expression of ER (P < 0.05). The expression of KAI1/CD82 and C-erbB-2 in breast cancer had no correlation. Conclusions: KAI1/CD82 and C-erbB-2 both participate in the proceeding of breast cancer,they may help to evaluate the invasiveness and metastasis of breast cancer.
Objective: To explore the expression of KAI1/CD82 and C-erbB-2 in breast cancer and its clinieal significance. Methods: The expression of KAI1/CD82,C-erbB-2 and estrogen receptor (ER) in 57 cases of breast cancer and 11 cases of benign mammary lesions were detected by immunohistochemistry S-P method,and their correlation with the clinicopathologic features was analyzed. Results: The positive rate of KAI1/CD82 was 31.58% in the breast cancer and 81.82% in the benign mammary lesions. The positive rate of C-erbB-2 was 71.93% in the breast cancer and zero in benign mammary lesions. The difference was statistically significant between the two groups (P < 0.01). The positive expression of KAI1/CD82 had statistical difference in cases with no metastasis of auxiliary lymph nodes and cases with negative expression of ER (P < 0.05). The positive expression of C-erbB-2 in cases with positive expression of ER was lower than that in cases with negative expression of ER (P < 0.05). The expression of KAI1/CD82 and C-erbB-2 in breast cancer had no correlation. Conclusions: KAI1/CD82 and C-erbB-2 both participate in the proceeding of breast cancer,they may help to evaluate the invasiveness and metastasis of breast cancer.
2011, 36(10): 1061-1063.
Abstract:
Objective: To investigate the analgesic effect of local application of ropivacaine to the neck muscle and the incision of cervical spondylosis on patients undergoing anterior and posterior combined approach. Methods: Forty patients with cervical spondylosis receiving anterior and posterior combined approach were randomly divided into control group and observation group. The patients in the observation group were injected with 20 ml 0.375% ropivacaine in the neck muscle and incision before operation,and applied additional spray of 20 ml to the neck muscle and incision after operation. The patients in the control group were given PCA analgesia pump containing flurbiprofen axetil and fentanyl after operation. The sedation scores and body comfort scores (BCS) at 0,2,6,12 and 24 hours after operation were evaluated. The pain in the neck,napex and incision was recorded according to the visual analog score (VAS). The adverse effects after operation were observed. Results: The VAS scores and BCS scores of the observation group at different time points within 12 hours were statistically different from those of the control group (P < 0.01),and those at 24 hours had no statistical difference (P > 0.05). The sedation scores of the observation group at different time points within 6 hours were statistically different from those of the control group (P < 0.01),and those after 12 hours had no statistical difference (P > 0.05). The incidence rate of the adverse effects had no statistical difference between the two groups (P > 0.05). Conclusions: Local application of ropivacaine can reduce the postoperative pain in cervical spondylosis patients undergoing anterior and posterior combined approach,and no obvious toxicity related to the use of ropivacaine has been observed.
Objective: To investigate the analgesic effect of local application of ropivacaine to the neck muscle and the incision of cervical spondylosis on patients undergoing anterior and posterior combined approach. Methods: Forty patients with cervical spondylosis receiving anterior and posterior combined approach were randomly divided into control group and observation group. The patients in the observation group were injected with 20 ml 0.375% ropivacaine in the neck muscle and incision before operation,and applied additional spray of 20 ml to the neck muscle and incision after operation. The patients in the control group were given PCA analgesia pump containing flurbiprofen axetil and fentanyl after operation. The sedation scores and body comfort scores (BCS) at 0,2,6,12 and 24 hours after operation were evaluated. The pain in the neck,napex and incision was recorded according to the visual analog score (VAS). The adverse effects after operation were observed. Results: The VAS scores and BCS scores of the observation group at different time points within 12 hours were statistically different from those of the control group (P < 0.01),and those at 24 hours had no statistical difference (P > 0.05). The sedation scores of the observation group at different time points within 6 hours were statistically different from those of the control group (P < 0.01),and those after 12 hours had no statistical difference (P > 0.05). The incidence rate of the adverse effects had no statistical difference between the two groups (P > 0.05). Conclusions: Local application of ropivacaine can reduce the postoperative pain in cervical spondylosis patients undergoing anterior and posterior combined approach,and no obvious toxicity related to the use of ropivacaine has been observed.
2011, 36(10): 1064-1066.
Abstract:
Objective: To investigate the relationship between the expression of human papilloma virus (HPV)16/18 and the clinicopathologic parameters in cervical carcinoma. Methods: S-P immuno-histochemical method was used to detect the expression of HPV 16/18 in 42 patients with cervical carcinomas and 30 normal cervical tissues. The association between the expression of HPV 16/18 and the clinicopathologic parameters (such as age,histologic grade,clinical stage and lymph node metastasis) in cervical carcinomas was assessed. Results: The expression of HPV 16/18 in cervical carcinoma was significant higher than that in normal cervical tissues (P < 0.01). The expression of HPV 16/18 in lymph node was correlated with the clinical stage and lymph node metastasis of cervical carcinoma (P=0.032),but not related to the tumor histological grade or patients'age (P > 0.05 and P=1.000). Conclusions: The expression of HPV 16/18 is correlated with the clinicopathologic parameters and prognosis of cervical carcinoma,and may serve as a useful reference index to judge the lymph node metastasis of the cervical carcinoma.
Objective: To investigate the relationship between the expression of human papilloma virus (HPV)16/18 and the clinicopathologic parameters in cervical carcinoma. Methods: S-P immuno-histochemical method was used to detect the expression of HPV 16/18 in 42 patients with cervical carcinomas and 30 normal cervical tissues. The association between the expression of HPV 16/18 and the clinicopathologic parameters (such as age,histologic grade,clinical stage and lymph node metastasis) in cervical carcinomas was assessed. Results: The expression of HPV 16/18 in cervical carcinoma was significant higher than that in normal cervical tissues (P < 0.01). The expression of HPV 16/18 in lymph node was correlated with the clinical stage and lymph node metastasis of cervical carcinoma (P=0.032),but not related to the tumor histological grade or patients'age (P > 0.05 and P=1.000). Conclusions: The expression of HPV 16/18 is correlated with the clinicopathologic parameters and prognosis of cervical carcinoma,and may serve as a useful reference index to judge the lymph node metastasis of the cervical carcinoma.
2011, 36(10): 1067-1068,1070.
Abstract:
Objective: To analyze the clinical features and treatment of lumbar intervertebral disc herniation in adolescents. Methods: The clinical data and imaging of 11 cases of lumbar intervertebral disc herniation in adolescents with the age of 15 to 18 were analyzed retrospectively. All the cases were performed interlaminar partial laminal fenestration after invalid conservative therapy. Results: All the cases were followed up from 0.5 to 2.5 years. The results were excellent in 8 cases and good in 2 cases. The excellent and good rates reached 90.90%. Conclusions: Trauma and malformation are the chief causes of the adolescent lumbar disc herniation. It presents obvious physical signs in clinical manifestation. The radiological diagnosis is nonspecific,but CT or MRI examination may help to make accurate diagnosis. If conservative therapy does not work,surgical therapy should be considered,which always has a good prognosis.
Objective: To analyze the clinical features and treatment of lumbar intervertebral disc herniation in adolescents. Methods: The clinical data and imaging of 11 cases of lumbar intervertebral disc herniation in adolescents with the age of 15 to 18 were analyzed retrospectively. All the cases were performed interlaminar partial laminal fenestration after invalid conservative therapy. Results: All the cases were followed up from 0.5 to 2.5 years. The results were excellent in 8 cases and good in 2 cases. The excellent and good rates reached 90.90%. Conclusions: Trauma and malformation are the chief causes of the adolescent lumbar disc herniation. It presents obvious physical signs in clinical manifestation. The radiological diagnosis is nonspecific,but CT or MRI examination may help to make accurate diagnosis. If conservative therapy does not work,surgical therapy should be considered,which always has a good prognosis.
2011, 36(10): 1069-1070.
Abstract:
Objective: To evaluate the efficacy of abdominal flap's repairing early wound of the hands by electric burns. Methods: Twenty-three cases were repaired with abdominal flaps in the early stage. Results: Flaps in 21 cases survived in good shape and function,2 cases were necrosis and liquefaction under the flaps because of debridement was not complete,but they were healed after drainage and resuture. Conclusions: The effect of the application of the abdominal flaps in repairing the early wound of the hands by electric burns is reliable.
Objective: To evaluate the efficacy of abdominal flap's repairing early wound of the hands by electric burns. Methods: Twenty-three cases were repaired with abdominal flaps in the early stage. Results: Flaps in 21 cases survived in good shape and function,2 cases were necrosis and liquefaction under the flaps because of debridement was not complete,but they were healed after drainage and resuture. Conclusions: The effect of the application of the abdominal flaps in repairing the early wound of the hands by electric burns is reliable.
2011, 36(10): 1071-1074.
Abstract:
Objective: To study the expressions and clinical correlations of Ki-67 and vascular endothelial growth factor (VEGF) in borderline ovarian tumors (BOT),in order to help differential diagnosis from other ovary tumors. Methods: Immunohistochemistry test (ElivisionTM plus method) was used to detect the expressions of Ki-67 and VEGF in 38 cases with BOT,compared to 30 cases with benign ovarian tumors and 30 cases with epithelial ovarian cancer. Results: The expressions of Ki-67 and VEGF in BOT,benign ovarian tumors and epithelial ovarian cancer were gradually increased (P < 0.01). The expression of Ki-67 was increased with the increment of VEGF expression. The expressions of Ki-67 and VEGF did not correlate to pathological type (P > 0.05). And there was a good unanimity and significant correlation between the expressions of Ki-67 and VEGF. Conclusions: The over-expressions of Ki-67 and VEGF in ovarian tumors indicated high malignant degree,and these changes may prompt the aggressive behavior. Combined detection with Ki-67 and VEGF may be a valuable marker for prediction of tumor progression and managements of ovarian tumors.
Objective: To study the expressions and clinical correlations of Ki-67 and vascular endothelial growth factor (VEGF) in borderline ovarian tumors (BOT),in order to help differential diagnosis from other ovary tumors. Methods: Immunohistochemistry test (ElivisionTM plus method) was used to detect the expressions of Ki-67 and VEGF in 38 cases with BOT,compared to 30 cases with benign ovarian tumors and 30 cases with epithelial ovarian cancer. Results: The expressions of Ki-67 and VEGF in BOT,benign ovarian tumors and epithelial ovarian cancer were gradually increased (P < 0.01). The expression of Ki-67 was increased with the increment of VEGF expression. The expressions of Ki-67 and VEGF did not correlate to pathological type (P > 0.05). And there was a good unanimity and significant correlation between the expressions of Ki-67 and VEGF. Conclusions: The over-expressions of Ki-67 and VEGF in ovarian tumors indicated high malignant degree,and these changes may prompt the aggressive behavior. Combined detection with Ki-67 and VEGF may be a valuable marker for prediction of tumor progression and managements of ovarian tumors.
2011, 36(10): 1075-1077.
Abstract:
Objective: To probe into the incidence of transient hypothyroxinemia (THP) in premature infants and to observe their early quality of life. Methods: One hundred and twenty-five hospitalized premature infants were divided into two groups. Group A included 56 premature infants with no other diseases,group B included 69 premature infants who were accompanied by other diseases,and 62 normal neonates acted as control. Electrochemistry luminescence was used to detect the blood thyroid-stimulating hormone (TSH),total thyroxine (TT4) and total triodothyroxine (TT3) of all the three groups on the 1st and 7th day. Results: The incidence of THP was 62.4% in the premature. The concentration of blood TSH,TT4 and TT3 on the 7th day was lower than that on the 1st day in all the three groups. The TSH,TT4 and TT3 in group A and B was lower than that of the control (P < 0.01);the TSH,TT4 and TT3 in group A showed no significant difference with group B on the 1st day (P < 0.01),but the TT4 in group B was lower than that in group A on the 7th day (P < 0.01);the TT4/TSH in the three groups showed no significant difference on the 1st day (P > 0.05),but on the 7th day,the TT4 and TSH in the control group were higher than those in group A and B (P < 0.01). The infants in THP group needed more oxygen,and the time of regular defecation,sustaining of hyperbilirubinemia,heat amounts to 418.4 kJ·kg-1·d-1 and restoring birth weight was delayed compared with the control (P < 0.01). Conclusions: The development of the thypothalamus-pituitary-thyroid gland of the premature infants is immature,which will influence their early quality of life.
Objective: To probe into the incidence of transient hypothyroxinemia (THP) in premature infants and to observe their early quality of life. Methods: One hundred and twenty-five hospitalized premature infants were divided into two groups. Group A included 56 premature infants with no other diseases,group B included 69 premature infants who were accompanied by other diseases,and 62 normal neonates acted as control. Electrochemistry luminescence was used to detect the blood thyroid-stimulating hormone (TSH),total thyroxine (TT4) and total triodothyroxine (TT3) of all the three groups on the 1st and 7th day. Results: The incidence of THP was 62.4% in the premature. The concentration of blood TSH,TT4 and TT3 on the 7th day was lower than that on the 1st day in all the three groups. The TSH,TT4 and TT3 in group A and B was lower than that of the control (P < 0.01);the TSH,TT4 and TT3 in group A showed no significant difference with group B on the 1st day (P < 0.01),but the TT4 in group B was lower than that in group A on the 7th day (P < 0.01);the TT4/TSH in the three groups showed no significant difference on the 1st day (P > 0.05),but on the 7th day,the TT4 and TSH in the control group were higher than those in group A and B (P < 0.01). The infants in THP group needed more oxygen,and the time of regular defecation,sustaining of hyperbilirubinemia,heat amounts to 418.4 kJ·kg-1·d-1 and restoring birth weight was delayed compared with the control (P < 0.01). Conclusions: The development of the thypothalamus-pituitary-thyroid gland of the premature infants is immature,which will influence their early quality of life.
2011, 36(10): 1078-1080,1083.
Abstract:
Objective: To explore the expression of survivin and p53 in epithelial borderline ovarian tumors and their clinical significance and correlation. Methods: Immunohistochemical EliVision method was used to detect the expression of survivin and p53 in 30 cases of epithelial ovarian carcinomas,30 cases of epithelial borderline ovarian tumors and 30 cases of epithelial ovarian benign tumors. Results: In epithelial benign ovarian tumors,epithelial borderline ovarian tumors and epithelial ovarian carcinomas,the positive rates of survivin immunostaining were 13.33%,43.33% and 86.67%,respectively;there were significant differences among the three groups (P < 0.05). The positive rates of p53 were 23.33%,36.67% and 73.33%,respectively;there was statistical difference between the epithelial ovarian carcinomas and the epithelial benign or borderline ovarian tumors (P < 0.05-P < 0.01),but no statistical difference was observed between the benign ovarian tumors and the borderline ovarian tumors (P > 0.05). There was a positive correlation between the expression of survivin and p53 in borderline ovarian tumors (P < 0.05). Conclusions: Over expression of survivin and p53 suggests that they may participate in the oncogensis and progression of epithelial borderline ovarian tumors,which can provide reference for the diagnosis and prognosis of the epithelial borderline ovarian tumors. Both survivin and p53 may play an important role in the pathogenesis of epithelial borderline ovarian tumors.
Objective: To explore the expression of survivin and p53 in epithelial borderline ovarian tumors and their clinical significance and correlation. Methods: Immunohistochemical EliVision method was used to detect the expression of survivin and p53 in 30 cases of epithelial ovarian carcinomas,30 cases of epithelial borderline ovarian tumors and 30 cases of epithelial ovarian benign tumors. Results: In epithelial benign ovarian tumors,epithelial borderline ovarian tumors and epithelial ovarian carcinomas,the positive rates of survivin immunostaining were 13.33%,43.33% and 86.67%,respectively;there were significant differences among the three groups (P < 0.05). The positive rates of p53 were 23.33%,36.67% and 73.33%,respectively;there was statistical difference between the epithelial ovarian carcinomas and the epithelial benign or borderline ovarian tumors (P < 0.05-P < 0.01),but no statistical difference was observed between the benign ovarian tumors and the borderline ovarian tumors (P > 0.05). There was a positive correlation between the expression of survivin and p53 in borderline ovarian tumors (P < 0.05). Conclusions: Over expression of survivin and p53 suggests that they may participate in the oncogensis and progression of epithelial borderline ovarian tumors,which can provide reference for the diagnosis and prognosis of the epithelial borderline ovarian tumors. Both survivin and p53 may play an important role in the pathogenesis of epithelial borderline ovarian tumors.
2011, 36(10): 1081-1083.
Abstract:
Objective: To investigate the correlation between nutrition status and thyroid hormone in the patients with chronic obstructive pulmonary disease (COPD). Methods: One hundred and forty-eight COPD patients in stable stage and 30 normal controls were studied. Nutritional parameters,including body mass index (BMI),percentage of normal body mass (NM%),triceps skin-fold thickness (TSF),mid-upper arm circumference (MAC),serum albumin (ALB),and total lymphocyte counts (LYM) were determined. COPD patients were divided into malnutrition group (groupⅠ,47 cases) and non-malnutrition group (group Ⅱ,101 cases) according to these nutritional parameters. Thyroid hormones (including T3,T4 and TSH) were detected by radioimmunoassay method. The correlation between these parameters were analyzed. Results: In contrast to control group and groupⅡ,there were significant differences on BMI,NW%,TSF,MAC,ALB,LYM,T3 and T4 in group Ⅰ(P < 0.01). Except that T4 in control group was higher than group Ⅱ,there were no significant difference on these parameters between the two groups (P > 0.05). Significant correlation was existed between T3 and all nutritional parameters (P < 0.05-P < 0.01),T4 was only linked to albumin (P < 0.05). Conclusions: There is significant correlation between thyronine and nutritional status in patients with COPD.
Objective: To investigate the correlation between nutrition status and thyroid hormone in the patients with chronic obstructive pulmonary disease (COPD). Methods: One hundred and forty-eight COPD patients in stable stage and 30 normal controls were studied. Nutritional parameters,including body mass index (BMI),percentage of normal body mass (NM%),triceps skin-fold thickness (TSF),mid-upper arm circumference (MAC),serum albumin (ALB),and total lymphocyte counts (LYM) were determined. COPD patients were divided into malnutrition group (groupⅠ,47 cases) and non-malnutrition group (group Ⅱ,101 cases) according to these nutritional parameters. Thyroid hormones (including T3,T4 and TSH) were detected by radioimmunoassay method. The correlation between these parameters were analyzed. Results: In contrast to control group and groupⅡ,there were significant differences on BMI,NW%,TSF,MAC,ALB,LYM,T3 and T4 in group Ⅰ(P < 0.01). Except that T4 in control group was higher than group Ⅱ,there were no significant difference on these parameters between the two groups (P > 0.05). Significant correlation was existed between T3 and all nutritional parameters (P < 0.05-P < 0.01),T4 was only linked to albumin (P < 0.05). Conclusions: There is significant correlation between thyronine and nutritional status in patients with COPD.
2011, 36(10): 1084-1086.
Abstract:
Objective: To evaluate the clinical significance of serum tumor markers carbohydrate antigen 19-9(CA19-9),carbohydrate antigen 242(CA242) and carcinoembryonic antigen (CEA) in differential diagnosis of benign and malignant obstructive jaundice. Methods: Serum levels of CA199,CA242 and CEA were detected by radioimmunoassay in 88 patients with malignant obstructive jaundice and 52 patients with benign obstructive jaundice. The receiver-operating characteristics curve (ROC) of the three serum tumor markers was made and the areas under the curve (AUC) were calculated. The sensitivity,specificity,positive predict value,negative predict value,positive likelihood ratio and negative likelihood ratio of CA199,CA242 and CEA were calculated and analyzed. Results: CA19-9,CA242 and CEA in patients with malignant obstructive jaundice were significantly higher than those in patients with benign obstructive jaundice (P < 0.01). The AUC of CA19-9,CA242 and CEA were 0.882,0.904 and 0.843,respectively. The positive likelihood ratio priority of CA19-9,CA242 and CEA were 1.96,6.04 and 10.44,respectively,and the negative likelihood ratio priority was 0.11,0.30 and 0.42,respectively. Serial combination testing increased the diagnostic specificity,and parallel combination testing increased the diagnostic sensitivity. Conclusions: Combined measurement of serum CA19-9,CA242 and CEA is valuable in differential diagnosis of benign and malignant obstructive jaundice.
Objective: To evaluate the clinical significance of serum tumor markers carbohydrate antigen 19-9(CA19-9),carbohydrate antigen 242(CA242) and carcinoembryonic antigen (CEA) in differential diagnosis of benign and malignant obstructive jaundice. Methods: Serum levels of CA199,CA242 and CEA were detected by radioimmunoassay in 88 patients with malignant obstructive jaundice and 52 patients with benign obstructive jaundice. The receiver-operating characteristics curve (ROC) of the three serum tumor markers was made and the areas under the curve (AUC) were calculated. The sensitivity,specificity,positive predict value,negative predict value,positive likelihood ratio and negative likelihood ratio of CA199,CA242 and CEA were calculated and analyzed. Results: CA19-9,CA242 and CEA in patients with malignant obstructive jaundice were significantly higher than those in patients with benign obstructive jaundice (P < 0.01). The AUC of CA19-9,CA242 and CEA were 0.882,0.904 and 0.843,respectively. The positive likelihood ratio priority of CA19-9,CA242 and CEA were 1.96,6.04 and 10.44,respectively,and the negative likelihood ratio priority was 0.11,0.30 and 0.42,respectively. Serial combination testing increased the diagnostic specificity,and parallel combination testing increased the diagnostic sensitivity. Conclusions: Combined measurement of serum CA19-9,CA242 and CEA is valuable in differential diagnosis of benign and malignant obstructive jaundice.
2011, 36(10): 1087-1089.
Abstract:
Objective: To study the value of cystatin C in monitoring the renal function of patients receiving chemotherapy with platinum containing regimens. Methods: The blood samples of the patients receiving chemotherapy with platinum containing regimens were collected before and 7 days after the therapy to detect the cystatin C and creatinine,and the correlation between them was also observed. Results: Thirty-seven patients received 74 cycles of chemotherapy in all. The correlation between serum Cr and Cystatin C was confirmed (r=0.398,P < 0.01);Cystatin C was correlated with creatinine significantly (r=0.412,P < 0.01),and its correlation with CCr was no better than creatinine (r=0.412 vs r=0.567,P > 0.05);the serum Cystatin C level of the patients was higher after chemotherapy than before (P < 0.01). Sixteen of the patients received chemotherapy with cisplatin for 32 cycles,and their serum Cystatin C levels increased (P < 0.01);21 patients received chemotherapy with non-cisplatin for 42 cycles,and the results were the same (P < 0.01). The serum creatinine concentrations were not significantly different before and after chemotherapy in the two groups (P > 0.05). The abnormal rate of Cystatin C in patients receiving chemotherapy with cisplatin was higher than that with non-cisplatin chemotherapy (P < 0.01). Creatinine was detected abnormal only in one case. Conclusions: Cystatin C presents a much more sensitive clinical marker than creatinine for early assessment of GFR damage caused by chemotherapy with platinum containing regimens,but as there are some difficulties in clinical application,it could not act as a substitute for creatinine.
Objective: To study the value of cystatin C in monitoring the renal function of patients receiving chemotherapy with platinum containing regimens. Methods: The blood samples of the patients receiving chemotherapy with platinum containing regimens were collected before and 7 days after the therapy to detect the cystatin C and creatinine,and the correlation between them was also observed. Results: Thirty-seven patients received 74 cycles of chemotherapy in all. The correlation between serum Cr and Cystatin C was confirmed (r=0.398,P < 0.01);Cystatin C was correlated with creatinine significantly (r=0.412,P < 0.01),and its correlation with CCr was no better than creatinine (r=0.412 vs r=0.567,P > 0.05);the serum Cystatin C level of the patients was higher after chemotherapy than before (P < 0.01). Sixteen of the patients received chemotherapy with cisplatin for 32 cycles,and their serum Cystatin C levels increased (P < 0.01);21 patients received chemotherapy with non-cisplatin for 42 cycles,and the results were the same (P < 0.01). The serum creatinine concentrations were not significantly different before and after chemotherapy in the two groups (P > 0.05). The abnormal rate of Cystatin C in patients receiving chemotherapy with cisplatin was higher than that with non-cisplatin chemotherapy (P < 0.01). Creatinine was detected abnormal only in one case. Conclusions: Cystatin C presents a much more sensitive clinical marker than creatinine for early assessment of GFR damage caused by chemotherapy with platinum containing regimens,but as there are some difficulties in clinical application,it could not act as a substitute for creatinine.
2011, 36(10): 1090-1091.
Abstract:
Objective: To observe the efficacy of repaglinide in treatment of newly diagnosed type 2 diabetes mellitus. Methods: Two hundred patients who were newly diagnosed to have type 2 diabetes mellifus were administered repaglinide 1.0 mg 3 times a day before meals in addition to the therapy of diet-control and adequate exercise. And the effect was observed in the following 16 weeks. The BMI,blood fat,fasting blood glucose,blood glucose 2 hours after meals,HbA1C and fasting insulin at 8 weeks and 16 weeks after the therapy were detected. Results: After 16 weeks of treatment,the fasting blood glucose,blood glucose 2 hours after meals and HbA1C were significantly decreased (P < 0.01). Conclusions: Repaglinide may effectively control the blood glucose of patients with type 2 diabetes with few adverse reactions and a low incidence of hypoglycemia.
Objective: To observe the efficacy of repaglinide in treatment of newly diagnosed type 2 diabetes mellitus. Methods: Two hundred patients who were newly diagnosed to have type 2 diabetes mellifus were administered repaglinide 1.0 mg 3 times a day before meals in addition to the therapy of diet-control and adequate exercise. And the effect was observed in the following 16 weeks. The BMI,blood fat,fasting blood glucose,blood glucose 2 hours after meals,HbA1C and fasting insulin at 8 weeks and 16 weeks after the therapy were detected. Results: After 16 weeks of treatment,the fasting blood glucose,blood glucose 2 hours after meals and HbA1C were significantly decreased (P < 0.01). Conclusions: Repaglinide may effectively control the blood glucose of patients with type 2 diabetes with few adverse reactions and a low incidence of hypoglycemia.
2011, 36(10): 1092-1094.
Abstract:
Objective: To investigate the safety and effect of palatorrhaphy in cleft palate repair on infants. Methods: One hundred and eighteen cases of infants,from 5 to 18 month,were included. Two-flap was adopted as main method in operation,but combined with method of vomer bone flap repaire in complete cleft palate and. The improved Langenbeck method was adopted in incomplete cleft palate and cleft soft palate. The high frequency electric knife was used to stop bleeding. The oropharynx ventiduct was detained in oral cavity after operation. Results: One hundred and eighteen cases were safety during the anesthesia and operation. There was no severe complication during and after the operation. The infants all received primary healing except 3 cases of incision erosion,2 cases of palate perforation. After followed up,nearly 90% infants in 87 cases received good speech sound recovery to or near the level of normal children after surgery. Conclusions: The infant palatorrhaphy is safety and effective,as long as a fine anesthesia and practiced operation are given.
Objective: To investigate the safety and effect of palatorrhaphy in cleft palate repair on infants. Methods: One hundred and eighteen cases of infants,from 5 to 18 month,were included. Two-flap was adopted as main method in operation,but combined with method of vomer bone flap repaire in complete cleft palate and. The improved Langenbeck method was adopted in incomplete cleft palate and cleft soft palate. The high frequency electric knife was used to stop bleeding. The oropharynx ventiduct was detained in oral cavity after operation. Results: One hundred and eighteen cases were safety during the anesthesia and operation. There was no severe complication during and after the operation. The infants all received primary healing except 3 cases of incision erosion,2 cases of palate perforation. After followed up,nearly 90% infants in 87 cases received good speech sound recovery to or near the level of normal children after surgery. Conclusions: The infant palatorrhaphy is safety and effective,as long as a fine anesthesia and practiced operation are given.
2011, 36(10): 1103-1104,1107.
Abstract:
Objective: To observe the accuracy of optical (PLT-O) method and electric impedance (PLT-I) method in counting the platelet by the XE-2100 automatic hematology analyzer. Methods: The platelet samples were collected from the high-value group (40 cases)(PLT > 300×109/L),median-value group (40 cases)(80×109/L < PLT ≤ 300×109/L) and low-value group (40 cases)(PLT ≤ 80×109/L). The platelet counting was conducted using PLT-O method and PLT-I method with the XE-2100 automatic hematology analyzer,and the results were compared with those by manual method. Results: The results obtained by PLT-O and PLT-I methods were consistent with those by manual method in the median-value group and the high-value platelet group (P > 0.05). However,of those lowvalue groups,the results of PLT-O are more consistent with the results of manual method than the one of PLT-I. Conclusions: The counting results by PLT-O and PLT-I methods using the XE-2100 hematology analyzer are consistent in the median-value and the highvalue platelet groups,but results by PLT-O have more important reference value in the re-examination to low-value platelet group.
Objective: To observe the accuracy of optical (PLT-O) method and electric impedance (PLT-I) method in counting the platelet by the XE-2100 automatic hematology analyzer. Methods: The platelet samples were collected from the high-value group (40 cases)(PLT > 300×109/L),median-value group (40 cases)(80×109/L < PLT ≤ 300×109/L) and low-value group (40 cases)(PLT ≤ 80×109/L). The platelet counting was conducted using PLT-O method and PLT-I method with the XE-2100 automatic hematology analyzer,and the results were compared with those by manual method. Results: The results obtained by PLT-O and PLT-I methods were consistent with those by manual method in the median-value group and the high-value platelet group (P > 0.05). However,of those lowvalue groups,the results of PLT-O are more consistent with the results of manual method than the one of PLT-I. Conclusions: The counting results by PLT-O and PLT-I methods using the XE-2100 hematology analyzer are consistent in the median-value and the highvalue platelet groups,but results by PLT-O have more important reference value in the re-examination to low-value platelet group.
2011, 36(10): 1105-1107.
Abstract:
Objective: To ensure the security of clinical blood transfusion by analyzing the causes of substandard filling of blood transfusion application forms. Methods: Two thousand eight hundred and sixty-four transfusion application forms collected from our department during Jan. 2009 to Dec. 2009 were checked and analyzed;integrity and standard filling of all the items were regarded as qualified forms. Results: One thousand two hundred and thirty-three (43.1%) of the transfusion application forms were substandardly filled,and 1 538 unstandard handwriting errors were detected. Conclusions: Substandardly filled transfusion application forms are frequently seen,which may be hidden danger to transfusion safety and lead to health disputes. It is necessary for medical workers to fill the transfusion application forms correctly to ensure the security of clinical blood transfusion.
Objective: To ensure the security of clinical blood transfusion by analyzing the causes of substandard filling of blood transfusion application forms. Methods: Two thousand eight hundred and sixty-four transfusion application forms collected from our department during Jan. 2009 to Dec. 2009 were checked and analyzed;integrity and standard filling of all the items were regarded as qualified forms. Results: One thousand two hundred and thirty-three (43.1%) of the transfusion application forms were substandardly filled,and 1 538 unstandard handwriting errors were detected. Conclusions: Substandardly filled transfusion application forms are frequently seen,which may be hidden danger to transfusion safety and lead to health disputes. It is necessary for medical workers to fill the transfusion application forms correctly to ensure the security of clinical blood transfusion.
2011, 36(10): 1108-1110.
Abstract:
Objective: To evaluate the clinical effect of L-form bacterium culture in urine samples from patients with urinary infection. Methods: Five hundred and ten urine specimens were examined with the routine and L-form bacterium culture,the isolated strains were tested with drug susceptibility test. Results: Two hundred and thirty out of 510 urine specimens were identified as bacterial growth. Of them,100 wild-type strains and 60 L-form strains alone were isolated,and both the wild-type and the L-form strains from 70 urine samples. The drug susceptibility test were displayed that all of the L-form strains were susceptible to macrolides and tetracycline antibiotics,while the wild-type to penicllium and cephem antibiotics. Conclusions: Most of the urinary infection may be attributed to Lform bacterium. Therefore,the L-form bacterium culture should be done as a routine test and it contributes to the clinical diagnosis and rational use of antibiotics.
Objective: To evaluate the clinical effect of L-form bacterium culture in urine samples from patients with urinary infection. Methods: Five hundred and ten urine specimens were examined with the routine and L-form bacterium culture,the isolated strains were tested with drug susceptibility test. Results: Two hundred and thirty out of 510 urine specimens were identified as bacterial growth. Of them,100 wild-type strains and 60 L-form strains alone were isolated,and both the wild-type and the L-form strains from 70 urine samples. The drug susceptibility test were displayed that all of the L-form strains were susceptible to macrolides and tetracycline antibiotics,while the wild-type to penicllium and cephem antibiotics. Conclusions: Most of the urinary infection may be attributed to Lform bacterium. Therefore,the L-form bacterium culture should be done as a routine test and it contributes to the clinical diagnosis and rational use of antibiotics.
2011, 36(10): 1110-1112.
Abstract:
Objective: To investigate the seven drug-resistance genes in meticillin-resistant coagulase negative staphylococcus (MRCNS) in Suzhou area. Methods: The drug-resistance genes of mecA,gyrA,qacA/B/C,qacA,ermA/B/C,ermB and TetM were determined with polymerase chain reaction in 133 strains of MRCNS. Results: In the 133 strains of MRCNS,the carrier rates of mecA,gyrA,TetM,qacA/B/C,ermB,qacA and ermA/B/C were 100.0%,47.4%,51.9%,45.1%,25.6%,24.8% and 12.0%,respectively. Conclusions: In Suzhou area of Anhui province,the carrier rates of mecA,qacA/B/C and qacA genes in MRCNS are similar to that of other domestic regions,but that of gyrA,and TetM genes are higher,and ermA/B/C and ermB lower.
Objective: To investigate the seven drug-resistance genes in meticillin-resistant coagulase negative staphylococcus (MRCNS) in Suzhou area. Methods: The drug-resistance genes of mecA,gyrA,qacA/B/C,qacA,ermA/B/C,ermB and TetM were determined with polymerase chain reaction in 133 strains of MRCNS. Results: In the 133 strains of MRCNS,the carrier rates of mecA,gyrA,TetM,qacA/B/C,ermB,qacA and ermA/B/C were 100.0%,47.4%,51.9%,45.1%,25.6%,24.8% and 12.0%,respectively. Conclusions: In Suzhou area of Anhui province,the carrier rates of mecA,qacA/B/C and qacA genes in MRCNS are similar to that of other domestic regions,but that of gyrA,and TetM genes are higher,and ermA/B/C and ermB lower.
2011, 36(10): 1113-1115.
Abstract:
Objective: To explore the resistance changes of Escherichia coli (E. coli) in urinary tract infection,and provide proofs about the reasonable use of drugs in clinic. Methods: Two hundred and thirty-four strains of E. coli in 2008 to 2010 annual urine specimen with extended-spectrum β-lactamase (ESBLs) detection and drug susceptibility test were detected,and the drug susceptibility results were analyzed. Results: The rate of enzyme production of E. coli showed a rising trend;the drug sensitivity test showed that the resistance existed in normal antibiotics was in different degrees except for Imipenem,and the rate of medical resisitance in ESBLs (+) bacteria was higher than in ESBLs (-). Conclusions: The resistance of urinary tract infection which caused by E. coli especially in ESBLs (+) strains is so serious. It should be strengthen in the detections of the medical resistance,which provide the references for the clinic rational drug use.
Objective: To explore the resistance changes of Escherichia coli (E. coli) in urinary tract infection,and provide proofs about the reasonable use of drugs in clinic. Methods: Two hundred and thirty-four strains of E. coli in 2008 to 2010 annual urine specimen with extended-spectrum β-lactamase (ESBLs) detection and drug susceptibility test were detected,and the drug susceptibility results were analyzed. Results: The rate of enzyme production of E. coli showed a rising trend;the drug sensitivity test showed that the resistance existed in normal antibiotics was in different degrees except for Imipenem,and the rate of medical resisitance in ESBLs (+) bacteria was higher than in ESBLs (-). Conclusions: The resistance of urinary tract infection which caused by E. coli especially in ESBLs (+) strains is so serious. It should be strengthen in the detections of the medical resistance,which provide the references for the clinic rational drug use.
2011, 36(10): 1115-1118,1121.
Abstract:
Objective: To explore the correlation between family factors together with parent-child attachment and the aggressive behaviors in middle school students so as to provide basis for prevention of the behavior. Methods: Two thousand six hundred and twenty-six students from 4 middle schools in Bengbu were investigated with stratified and random cluster sampling method. All the students were surveyed by a self-designed questionnaire,Aggressive Questionnaire and Inventory of Parents. Results: The students'grade,gender,parental occupation,rearing pattern,attitude and parent-child attachment all affected the aggressive behavior of the middle school students to some extent (P < 0.05 to P < 0.01). Multiple linear regression analysis indicated that the main influential factors for middle school students'aggressive behaviors were parental alienation (P < 0.01). Conclusions: The middle school students'aggressive behaviors are related with family factors,and the degree of parent-child attachment and parental alienation are risk factors for the aggressive behaviors of middle school students.
Objective: To explore the correlation between family factors together with parent-child attachment and the aggressive behaviors in middle school students so as to provide basis for prevention of the behavior. Methods: Two thousand six hundred and twenty-six students from 4 middle schools in Bengbu were investigated with stratified and random cluster sampling method. All the students were surveyed by a self-designed questionnaire,Aggressive Questionnaire and Inventory of Parents. Results: The students'grade,gender,parental occupation,rearing pattern,attitude and parent-child attachment all affected the aggressive behavior of the middle school students to some extent (P < 0.05 to P < 0.01). Multiple linear regression analysis indicated that the main influential factors for middle school students'aggressive behaviors were parental alienation (P < 0.01). Conclusions: The middle school students'aggressive behaviors are related with family factors,and the degree of parent-child attachment and parental alienation are risk factors for the aggressive behaviors of middle school students.
2011, 36(10): 1119-1121.
Abstract:
Objective: To explore the correlation between college students'interpersonal trust and parental rearing pattern. Methods: Three hundred and seventeen college students were surveyed by Interpersonal Trust Scale and Egma Minnen av Bardndosnauppforstran. Results: Significant differences were observed in the only-child or not,as well as students from different resident places (P < 0.05 and P < 0.01). Interpersonal trust was positively correlated with the emotional warmth,and negatively correlated with parents'uncharitable punishment and rejection (P < 0.05-P < 0.01). Stepwise regression analysis showed that multivariable introducing equation of upbringing factors included father's warmth and excessive protection,mother's refusal and denying attitude (P < 0.05-P < 0.01). Conclusions: Parental rearing patterns can affect college students'interpersonal trust. Proper patterns of parental rearing can help to improve their interpersonal trust.
Objective: To explore the correlation between college students'interpersonal trust and parental rearing pattern. Methods: Three hundred and seventeen college students were surveyed by Interpersonal Trust Scale and Egma Minnen av Bardndosnauppforstran. Results: Significant differences were observed in the only-child or not,as well as students from different resident places (P < 0.05 and P < 0.01). Interpersonal trust was positively correlated with the emotional warmth,and negatively correlated with parents'uncharitable punishment and rejection (P < 0.05-P < 0.01). Stepwise regression analysis showed that multivariable introducing equation of upbringing factors included father's warmth and excessive protection,mother's refusal and denying attitude (P < 0.05-P < 0.01). Conclusions: Parental rearing patterns can affect college students'interpersonal trust. Proper patterns of parental rearing can help to improve their interpersonal trust.
2011, 36(10): 1122-1124.
Abstract:
Objective: To evaluate the effect of the teaching reform carried out in medical statistics laboratory courses in preventive medical undergraduates. Methods: A survey was conducted in two classes including 99 students enrolled in Bengbu Medical College majored in preventive medicine. One class served as the experimental group and the other as the control group. TCE was adopted in the experimental classes,and the effect was evaluated by the results of the students'final examination and the questionnaires. Results: The final examination score of the experimental group was obviously higher than that of the control groups (P < 0.01);the survey results showed that the students ability in communication,self-teaching,expression,information-acquisition and problem-solving in the experiment group was higher than that in the control group (P < 0.01). Conclusions: TCE teaching models may stimulate the students'interest in medical statistics laboratory courses and enhance their ability of settling problems in practice.
Objective: To evaluate the effect of the teaching reform carried out in medical statistics laboratory courses in preventive medical undergraduates. Methods: A survey was conducted in two classes including 99 students enrolled in Bengbu Medical College majored in preventive medicine. One class served as the experimental group and the other as the control group. TCE was adopted in the experimental classes,and the effect was evaluated by the results of the students'final examination and the questionnaires. Results: The final examination score of the experimental group was obviously higher than that of the control groups (P < 0.01);the survey results showed that the students ability in communication,self-teaching,expression,information-acquisition and problem-solving in the experiment group was higher than that in the control group (P < 0.01). Conclusions: TCE teaching models may stimulate the students'interest in medical statistics laboratory courses and enhance their ability of settling problems in practice.
2011, 36(10): 1127-1128.
Abstract:
Objective: To provide the evidence which help nurse change the healthy diet by investigating the status of breakfast of nurses. Methods: The nutrition and breakfast behavior of 542 nurses were investigated and analyzed through the self-designed questionnaires. Results: It showed that 96.9% nurses believed the importance of breakfast practice and 84.7% of those had breakfast everyday. Foods contained water-rice,milk,bread,dumpling and cake. Less carbohydrate and protein were given and no fruits and vegetables. Eight-two point seven percentage nurses were hungry in the morning and 14.6% of those were suffered from hypoglycemia,almost happened between 10:00 to 11:00,about 57.8%. Conclusions: A single species and inadequate nutrition are harmful for nurses. Rationally readjusting the dietary pattern is recommended.
Objective: To provide the evidence which help nurse change the healthy diet by investigating the status of breakfast of nurses. Methods: The nutrition and breakfast behavior of 542 nurses were investigated and analyzed through the self-designed questionnaires. Results: It showed that 96.9% nurses believed the importance of breakfast practice and 84.7% of those had breakfast everyday. Foods contained water-rice,milk,bread,dumpling and cake. Less carbohydrate and protein were given and no fruits and vegetables. Eight-two point seven percentage nurses were hungry in the morning and 14.6% of those were suffered from hypoglycemia,almost happened between 10:00 to 11:00,about 57.8%. Conclusions: A single species and inadequate nutrition are harmful for nurses. Rationally readjusting the dietary pattern is recommended.
2011, 36(10): 1129-1131.
Abstract:
Objective: To evaluate the value of MRI in determining the sort and degree of meniscus injury. Methods: The MRI of 208 cases of meniscus injury was reviewed. Results: Among the 208 cases,137 of the injury were seen in the wall of the meniscus and 71 in the lateral side. Thirty-one cases were determined as meniscus injury degreeⅠ,94 as degree Ⅱ and 83 as degree Ⅲ by arthroscopy. Eighty-three cases were proved to be meniscal tear by surgery or arthroscopy. Conclusions: MRI can clearly demonstrate the meniscus injury in the knee. It is considered to be a good method in diagnosing meniscus injury.
Objective: To evaluate the value of MRI in determining the sort and degree of meniscus injury. Methods: The MRI of 208 cases of meniscus injury was reviewed. Results: Among the 208 cases,137 of the injury were seen in the wall of the meniscus and 71 in the lateral side. Thirty-one cases were determined as meniscus injury degreeⅠ,94 as degree Ⅱ and 83 as degree Ⅲ by arthroscopy. Eighty-three cases were proved to be meniscal tear by surgery or arthroscopy. Conclusions: MRI can clearly demonstrate the meniscus injury in the knee. It is considered to be a good method in diagnosing meniscus injury.
2011, 36(10): 1135-1137.
Abstract:
Objective: To study the age role in the adverse reaction of patient-controlled analgesia (PCA) by elderly hip surgical patients. Methods: The incidence of the adverse reaction was retrospectively analyzed in different age groups (60-70 years old groups and more than 70 groups years old) of elderly hip surgical patients who accepted PCA or not. Results: The overall incidence of the adverse reaction in elderly hip surgical patients was not significantly different between the ones who accepted PCA and the ones who not accepted PCA (P > 0.05). In the 60-70 years old age group,the incidence of the adverse reaction was not significantly different between the ones who accepted PCA and the ones who not accepted PCA (P > 0.05);But in the elderly hip surgical patients whose age more than 70 years old,the incidence of the adverse reaction in patients who accepted PCA was significantly higher than that in the ones who not accepted PCA (P < 0.05). On the other hand,in the elderly hip surgical patients who accepted PCA,the incidence of the adverse reaction in the ones whose age more than 70 years old was significantly higher than that in the 60-70 years old age group (P < 0.05). In the elderly hip surgical patients who not accepted PCA,the incidence of the adverse reaction in different age groups was not significantly different (P > 0.05). Conclusions: The age is an important factor led to the adverse reaction of PCA in the elderly hip surgical patients whose age more than 70 years old.
Objective: To study the age role in the adverse reaction of patient-controlled analgesia (PCA) by elderly hip surgical patients. Methods: The incidence of the adverse reaction was retrospectively analyzed in different age groups (60-70 years old groups and more than 70 groups years old) of elderly hip surgical patients who accepted PCA or not. Results: The overall incidence of the adverse reaction in elderly hip surgical patients was not significantly different between the ones who accepted PCA and the ones who not accepted PCA (P > 0.05). In the 60-70 years old age group,the incidence of the adverse reaction was not significantly different between the ones who accepted PCA and the ones who not accepted PCA (P > 0.05);But in the elderly hip surgical patients whose age more than 70 years old,the incidence of the adverse reaction in patients who accepted PCA was significantly higher than that in the ones who not accepted PCA (P < 0.05). On the other hand,in the elderly hip surgical patients who accepted PCA,the incidence of the adverse reaction in the ones whose age more than 70 years old was significantly higher than that in the 60-70 years old age group (P < 0.05). In the elderly hip surgical patients who not accepted PCA,the incidence of the adverse reaction in different age groups was not significantly different (P > 0.05). Conclusions: The age is an important factor led to the adverse reaction of PCA in the elderly hip surgical patients whose age more than 70 years old.
2011, 36(10): 1138-1141.
Abstract:
Objective: To investigate the learning strategy level in five-year higher vocational nursing students and analyze the characteristics and differences,as well as its influencing factors,which serves as the basis of teaching guidance and learning strategy adjustment,and promoting the development of the students'learning ability. Methods: Both quantitative and qualitative research methods were used in the study of the learning strategies of 224 five-year higher vocational nursing students on a random basis. Results: A relatively low level of learning strategy was found during the study. The level of learning strategy hadn't been raised with grade;there had significant differences in time management,information processing,learning aids and self test among students in different grades (P < 0.01);and there had also statistical differences in the students attitudes,attentiveness and choices of main points (P < 0.05). Conclusions: The current levels of learning strategies among higher vocational nursing students were relatively low. Therefore,more guidance should be given in raising the students'professional awareness and improving the learning strategies guideness,in an effort to encourage the development of self-learning and life-long learning ability in higher vocational nursing students.
Objective: To investigate the learning strategy level in five-year higher vocational nursing students and analyze the characteristics and differences,as well as its influencing factors,which serves as the basis of teaching guidance and learning strategy adjustment,and promoting the development of the students'learning ability. Methods: Both quantitative and qualitative research methods were used in the study of the learning strategies of 224 five-year higher vocational nursing students on a random basis. Results: A relatively low level of learning strategy was found during the study. The level of learning strategy hadn't been raised with grade;there had significant differences in time management,information processing,learning aids and self test among students in different grades (P < 0.01);and there had also statistical differences in the students attitudes,attentiveness and choices of main points (P < 0.05). Conclusions: The current levels of learning strategies among higher vocational nursing students were relatively low. Therefore,more guidance should be given in raising the students'professional awareness and improving the learning strategies guideness,in an effort to encourage the development of self-learning and life-long learning ability in higher vocational nursing students.
2011, 36(10): 1143-1145.
Abstract:
Objective: To propose treatment that improving life quality in patients with chronic respiratory failure through investigation of health education demand. Methods: Questionnaire method was used to investigate the demand of health education in the patients with chronic bronchitis. In addition,several relevant method and contents were made for education. Results: Patients with chronic bronchitis were desired to obtain health education to enhance self-healthy care. Developing effective health education reduced the recurrence of chronic respiratory failure. Conclusions: Health education can decrease the recurrence of chronic respiratory failure,postpone progress of the disease and improve quality of life of patients in this study.
Objective: To propose treatment that improving life quality in patients with chronic respiratory failure through investigation of health education demand. Methods: Questionnaire method was used to investigate the demand of health education in the patients with chronic bronchitis. In addition,several relevant method and contents were made for education. Results: Patients with chronic bronchitis were desired to obtain health education to enhance self-healthy care. Developing effective health education reduced the recurrence of chronic respiratory failure. Conclusions: Health education can decrease the recurrence of chronic respiratory failure,postpone progress of the disease and improve quality of life of patients in this study.
2011, 36(10): 1147-1149.
Abstract:
Objective: To explore the role of three-way pipe in transurethral resection of prostate (TURP) and to sum up the nursing experience during operation. Methods: Forty-six cases in the experimental group were performed routine bladder colostomy followed by the drainage rinses and 48 cases in the control group were contacted directly to the flow pipe. The operation time,frequency of intraoperational drainage tube jam and bleeding volume during operation were recorded. Results: The average operation time was 110 minutes in the control group and 90 minutes in the experimental group. Intraoperational drainage tube jam occurred for 96 times in the experimental group and 1 time in the control group. The average bleeding volume was 466 ml in the control group and it was only 46 ml in the experimental group. The difference was significant (P < 0.05). Conclusions: The effect of three-way pipe is satisfactory for drainage in TURP and is easy to popularize.
Objective: To explore the role of three-way pipe in transurethral resection of prostate (TURP) and to sum up the nursing experience during operation. Methods: Forty-six cases in the experimental group were performed routine bladder colostomy followed by the drainage rinses and 48 cases in the control group were contacted directly to the flow pipe. The operation time,frequency of intraoperational drainage tube jam and bleeding volume during operation were recorded. Results: The average operation time was 110 minutes in the control group and 90 minutes in the experimental group. Intraoperational drainage tube jam occurred for 96 times in the experimental group and 1 time in the control group. The average bleeding volume was 466 ml in the control group and it was only 46 ml in the experimental group. The difference was significant (P < 0.05). Conclusions: The effect of three-way pipe is satisfactory for drainage in TURP and is easy to popularize.
Email
RSS