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2011 Vol. 36, No. 12
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2011, 36(12): 1289-1292,1295.
Abstract:
Objective:To investigate the effects of heparanase(HPA) gene silencing by miRNA interference on invasion and migration of GBC-SD cells of gallbladder carcinoma. Methods:Four pairs of single-stranded recombinant plasmid miRNA oligo-DNA targeting human HPA gene sequence were synthesized and transfected into GBC-SD cells by lipofectamineTM 2000. HPA mRNA expression was detectede by RT-PCR before and after transfection, the recombinant plasmid which showed the best silencing effect for further experiments was selected. Transwell invasion assays and wound scarification assays were performed to examine the migration and invasive ability of GBC-SD cells. Results:Recombinant plasmid pcDNA6.2-GW/EmGFP-miR-HPA-2 showed the best silencing effects. The migration distance in wound scarification assays of GBC-SD cells transfected with recombinant plasmid pcDNA 6.2-GW/EmGFP-miR-HPA-2 at 12h,24h was significant shorter than negative control and blank groups(P<0.05-P<0.01). The invasive ability of GBC-SD cells transfected with pcDNA6.2-GW/EmGFP-miR-HPA-2 was decreased significantly when compared with negative control and blank groups(P<0.01). Conclusions:The GBC-SD cells transfected with recombinant plasmid pcDNA6.2-GW/EmGFPmiR showed decreased HPA mRNA expression,as well as inhibited invasion and migration in vitro.
Objective:To investigate the effects of heparanase(HPA) gene silencing by miRNA interference on invasion and migration of GBC-SD cells of gallbladder carcinoma. Methods:Four pairs of single-stranded recombinant plasmid miRNA oligo-DNA targeting human HPA gene sequence were synthesized and transfected into GBC-SD cells by lipofectamineTM 2000. HPA mRNA expression was detectede by RT-PCR before and after transfection, the recombinant plasmid which showed the best silencing effect for further experiments was selected. Transwell invasion assays and wound scarification assays were performed to examine the migration and invasive ability of GBC-SD cells. Results:Recombinant plasmid pcDNA6.2-GW/EmGFP-miR-HPA-2 showed the best silencing effects. The migration distance in wound scarification assays of GBC-SD cells transfected with recombinant plasmid pcDNA 6.2-GW/EmGFP-miR-HPA-2 at 12h,24h was significant shorter than negative control and blank groups(P<0.05-P<0.01). The invasive ability of GBC-SD cells transfected with pcDNA6.2-GW/EmGFP-miR-HPA-2 was decreased significantly when compared with negative control and blank groups(P<0.01). Conclusions:The GBC-SD cells transfected with recombinant plasmid pcDNA6.2-GW/EmGFPmiR showed decreased HPA mRNA expression,as well as inhibited invasion and migration in vitro.
2011, 36(12): 1293-1295.
Abstract:
Objective:To observe the oxidative damage of rat liver mitochondria induced by different doses of manganese and explore the related mechanism. Methods:Twenty four male SD rats were divided into 4 groups,6 rats in each group,including control group,low dose group,medium dose group and high dose group. The rats in control group were injected normal saline intraperitoneally and the rats in low dose, medium and high dose groups were injected 2 g·kg-1·d-1, 8 g·kg-1·d-1 and 32 g·kg-1·d-1 MnCl2 intraperitoneally respectively for 30 days. The rat liver tissue was extracted and mitochondria suspension was obtained by differential centrifugation separation to measure mitochondrial PT pore,the degree of mitochondrial swelling,the mitochondrial membrane potential and the content of succinate dehydrogenase (SDH), cytochrome C, hydroxyl radical OH·and glutathione peroxidase (GSH-PX). Results:The mitochondrial PT pore in the four groups was different and the differences had statistical significances(P<0.01); the mitochondrial swelling degree in high dose group was lower than that in control and low dose group(P<0.05); the mitochondrial membrane potential in high dose group was decreased compared with that of other groups(P<0.05-P<0.01); the content of SDH in medium and high dose group was decreased compared with that in control and low dose group(P<0.01) and the difference of SDH between medium and high dose group had statistical significance(P<0.01); the cytochrome C content in high dose group was higher than that in control and low dose group(P<0.05); the content of hydroxyl radical OH·in high dose group was increased compared with that in other three groups (P<0.01); in the same time, the content of GSH-PX in low, medium and high groups decreased compared with that in control group(P<0.05). Conclusions:Manganese can induce mitochondrial oxidative damage. Manganese has the toxic effects on rat mitochondria and the toxic effects induce rat liver mitochondrial PT pore opening,decreasing the optical density of membrane potential and the content of SDH and GSH-PX,increasing the content of cytochrome C and hydroxyl radical OH·significantly.
Objective:To observe the oxidative damage of rat liver mitochondria induced by different doses of manganese and explore the related mechanism. Methods:Twenty four male SD rats were divided into 4 groups,6 rats in each group,including control group,low dose group,medium dose group and high dose group. The rats in control group were injected normal saline intraperitoneally and the rats in low dose, medium and high dose groups were injected 2 g·kg-1·d-1, 8 g·kg-1·d-1 and 32 g·kg-1·d-1 MnCl2 intraperitoneally respectively for 30 days. The rat liver tissue was extracted and mitochondria suspension was obtained by differential centrifugation separation to measure mitochondrial PT pore,the degree of mitochondrial swelling,the mitochondrial membrane potential and the content of succinate dehydrogenase (SDH), cytochrome C, hydroxyl radical OH·and glutathione peroxidase (GSH-PX). Results:The mitochondrial PT pore in the four groups was different and the differences had statistical significances(P<0.01); the mitochondrial swelling degree in high dose group was lower than that in control and low dose group(P<0.05); the mitochondrial membrane potential in high dose group was decreased compared with that of other groups(P<0.05-P<0.01); the content of SDH in medium and high dose group was decreased compared with that in control and low dose group(P<0.01) and the difference of SDH between medium and high dose group had statistical significance(P<0.01); the cytochrome C content in high dose group was higher than that in control and low dose group(P<0.05); the content of hydroxyl radical OH·in high dose group was increased compared with that in other three groups (P<0.01); in the same time, the content of GSH-PX in low, medium and high groups decreased compared with that in control group(P<0.05). Conclusions:Manganese can induce mitochondrial oxidative damage. Manganese has the toxic effects on rat mitochondria and the toxic effects induce rat liver mitochondrial PT pore opening,decreasing the optical density of membrane potential and the content of SDH and GSH-PX,increasing the content of cytochrome C and hydroxyl radical OH·significantly.
2011, 36(12): 1296-1298.
Abstract:
Objective:To investigate the expressions of AMP-activated protein kinase (AMPK) and LKB1 in adipose tissues of normal-fed rats and high-fat-fed rats,and explore the impact of AMPK and LKB1 on the metabolism of glucose and lipid. Methods:Thirty male six-week age Sprague-Dawley rats were randomly divided into two groups:15 were fed with normal control diet as NC group, the rest 15 were fed with a high-fat diet as HF group. After 16 weeks,the average body weight(BW) in HF group were over 20% higher than in NC group. All rats were weighed under anesthesia. The levels of free fatty acid (FFA),triglyceride (TG), total cholesterol (TCH),fasting plasma glucose(FPG) and fasting insulin(FINS) in serum were measured. After all rats were killed,the protein levels of AMPKα,P-AMPKα and P-LKB1 in adipose tissues were analyzed with Western blot. Results:Compared with NC group,the levels of BW,FFA,TG,FPG and FINS in HF group were significantly higher(P<0.05). In adipose tissues,the expressions of P-AMPKα and PLKB1 in HF group were lower than those in NC group(P<0.05). Conclusions:High-fat nutrition diet induces the activities of LKB1 and AMPK,promoteds the synthesis of TG,increases blood glucose,induces obesity and insulin resistance in rats.
Objective:To investigate the expressions of AMP-activated protein kinase (AMPK) and LKB1 in adipose tissues of normal-fed rats and high-fat-fed rats,and explore the impact of AMPK and LKB1 on the metabolism of glucose and lipid. Methods:Thirty male six-week age Sprague-Dawley rats were randomly divided into two groups:15 were fed with normal control diet as NC group, the rest 15 were fed with a high-fat diet as HF group. After 16 weeks,the average body weight(BW) in HF group were over 20% higher than in NC group. All rats were weighed under anesthesia. The levels of free fatty acid (FFA),triglyceride (TG), total cholesterol (TCH),fasting plasma glucose(FPG) and fasting insulin(FINS) in serum were measured. After all rats were killed,the protein levels of AMPKα,P-AMPKα and P-LKB1 in adipose tissues were analyzed with Western blot. Results:Compared with NC group,the levels of BW,FFA,TG,FPG and FINS in HF group were significantly higher(P<0.05). In adipose tissues,the expressions of P-AMPKα and PLKB1 in HF group were lower than those in NC group(P<0.05). Conclusions:High-fat nutrition diet induces the activities of LKB1 and AMPK,promoteds the synthesis of TG,increases blood glucose,induces obesity and insulin resistance in rats.
2011, 36(12): 1299-1302.
Abstract:
Objective:To investigate the expression of hypoxia inducible factor-1α(HIF-1α) and vascular endothelial growth factor-C (VEGF-C) and their relation to lymphangiogenesis and lymphatic metastasis in colorectal cancer. Methods:The expression of HIF-1α and VEGF-C in colorectal cancer tissues and normal colorectal tissues were detected in 72 patients with immunohistochemical EliVsion plus method and microlymphtic vessel density in VEGFR-3 staining was calculated. Results:The positive rates of HIF-1α and VEGF-C expressions density in colorectal cancer and adjacent normal tissue were 77.8%,83.0% and 44.4%,42.0%,respectively,there were the statistical differences(P<0.01). The positive rate of HIF-1α was 90.0% in lymph node metastasis cases,no transfer for 63.5%, the difference had statistical significance(P<0.01). The positive rate of VEGF-C was 95.0% in lymph node metastasis cases,no transfer for 68.8%,which had the statistical difference(χ2=7.78,P<0.01); the positive rates of HIF-1α were 12% and 44%,which had the statistical significance (χ2=8.82, P<0.01). In high, medium differentiation of colorectal cancer group and low, undifferentiated group,the positive rates of VEGF-C was 14% and 46%,respectively,also had the statistical difference(P<0.01). The expression of HIF-1α and VEGF-C in colorectal cancer tissues had synergistic effect (r=0.105, P<0.01). Conclusions:The expression of HIF-1α and VEGF-C are high in colorectal cancer,and low in adjacent tissues. The positive expression is associated with lymph node metastasis and the differentiation degree in carcinoma tissues,and has no relationship with age and sex. The expression of HIF-1α and VEGF-C are associated with each other in colorectal cancer tissues, and exists the synergy effect in colorectal cancer metastasis process.
Objective:To investigate the expression of hypoxia inducible factor-1α(HIF-1α) and vascular endothelial growth factor-C (VEGF-C) and their relation to lymphangiogenesis and lymphatic metastasis in colorectal cancer. Methods:The expression of HIF-1α and VEGF-C in colorectal cancer tissues and normal colorectal tissues were detected in 72 patients with immunohistochemical EliVsion plus method and microlymphtic vessel density in VEGFR-3 staining was calculated. Results:The positive rates of HIF-1α and VEGF-C expressions density in colorectal cancer and adjacent normal tissue were 77.8%,83.0% and 44.4%,42.0%,respectively,there were the statistical differences(P<0.01). The positive rate of HIF-1α was 90.0% in lymph node metastasis cases,no transfer for 63.5%, the difference had statistical significance(P<0.01). The positive rate of VEGF-C was 95.0% in lymph node metastasis cases,no transfer for 68.8%,which had the statistical difference(χ2=7.78,P<0.01); the positive rates of HIF-1α were 12% and 44%,which had the statistical significance (χ2=8.82, P<0.01). In high, medium differentiation of colorectal cancer group and low, undifferentiated group,the positive rates of VEGF-C was 14% and 46%,respectively,also had the statistical difference(P<0.01). The expression of HIF-1α and VEGF-C in colorectal cancer tissues had synergistic effect (r=0.105, P<0.01). Conclusions:The expression of HIF-1α and VEGF-C are high in colorectal cancer,and low in adjacent tissues. The positive expression is associated with lymph node metastasis and the differentiation degree in carcinoma tissues,and has no relationship with age and sex. The expression of HIF-1α and VEGF-C are associated with each other in colorectal cancer tissues, and exists the synergy effect in colorectal cancer metastasis process.
2011, 36(12): 1303-1305.
Abstract:
Objective:To evaluate and compare the urodynamic parameters and continence rate of urine reservoir function among patients who had undergone orthotopic bladder substitution with sigmoid or ileal segments. Methods:Continent urinary reservoirs were constructed in 73 patients. Thirty-three patients were received a sigmoid neobladder (SN) and 40 patients with an ileal neobladder (IN). All patients were examined urodynamically and taken intravenous pyelography to test their continence status. Urodynamic parameters and continence were obtained. Results:Complete follow-up was performed in 73 cases 12 months averagely postoperatively. The patients were examined after 3-6 months. There was no perioperative fatality cases. The average reservoir capacity of IN(485±60) ml was higher than of SN(350±52) ml(P<0.01),the bladder filling pressure and urination bladder pressure were lower than of IN(P<0.01). The urination continence ability was satisfied in the two groups. Daytime continence was 87.5% in IN group and 90.9% in SN group,nighttime continence was 65.0% in IN group and 48.5% in SN group(P>0.05). Two cases of hydronephrosis with mild impaired renal function were found in SN. Conclusions:A neobladder constructed from detubularized ileum or sigmoid achieves adequate reservoir capacity,accepted urodynamics with a satisfactory daytime continence rate. Nighttime incontinence in patients with IN and SN is still a vexed problem.
Objective:To evaluate and compare the urodynamic parameters and continence rate of urine reservoir function among patients who had undergone orthotopic bladder substitution with sigmoid or ileal segments. Methods:Continent urinary reservoirs were constructed in 73 patients. Thirty-three patients were received a sigmoid neobladder (SN) and 40 patients with an ileal neobladder (IN). All patients were examined urodynamically and taken intravenous pyelography to test their continence status. Urodynamic parameters and continence were obtained. Results:Complete follow-up was performed in 73 cases 12 months averagely postoperatively. The patients were examined after 3-6 months. There was no perioperative fatality cases. The average reservoir capacity of IN(485±60) ml was higher than of SN(350±52) ml(P<0.01),the bladder filling pressure and urination bladder pressure were lower than of IN(P<0.01). The urination continence ability was satisfied in the two groups. Daytime continence was 87.5% in IN group and 90.9% in SN group,nighttime continence was 65.0% in IN group and 48.5% in SN group(P>0.05). Two cases of hydronephrosis with mild impaired renal function were found in SN. Conclusions:A neobladder constructed from detubularized ileum or sigmoid achieves adequate reservoir capacity,accepted urodynamics with a satisfactory daytime continence rate. Nighttime incontinence in patients with IN and SN is still a vexed problem.
2011, 36(12): 1306-1308,1311.
Abstract:
Objective:To observe the expression of inducible nitric oxide synthases(iNOS) in laryngocarcinoma tissues and its role in the invasion and metastasis of laryngeal carcinoma. Methods:The expression of iNOS in 66 cases of laryngealcarcinoma tissues and the normal mucous membrane were detecdted by immunohistochemistry S-P method. The correlation between the expression of iNOS and the clinical stage,clinical classification,pathological grade and lymph node metastasis of laryngeal carcinoma was analyzed. Results:The positive rate of iNOS in laryngealcarcinoma tissues and the normal mucous membrance was 81.82% and 6.06%,respectively(P<0.05); the expression of iNOS was significantly correlated with the clinical stage and classification of patients with laryngeal carcinoma (P<0.05),but had no relation with the patients'age,pathological grade or lymph node metastasis(P>0.05). Conclusions:iNOS may act as an important tumor marker in lymph node metastasis of laryngeal carcinoma. It plays an important role in the invasion,metastasis and prognosis of laryngeal carcinoma.
Objective:To observe the expression of inducible nitric oxide synthases(iNOS) in laryngocarcinoma tissues and its role in the invasion and metastasis of laryngeal carcinoma. Methods:The expression of iNOS in 66 cases of laryngealcarcinoma tissues and the normal mucous membrane were detecdted by immunohistochemistry S-P method. The correlation between the expression of iNOS and the clinical stage,clinical classification,pathological grade and lymph node metastasis of laryngeal carcinoma was analyzed. Results:The positive rate of iNOS in laryngealcarcinoma tissues and the normal mucous membrance was 81.82% and 6.06%,respectively(P<0.05); the expression of iNOS was significantly correlated with the clinical stage and classification of patients with laryngeal carcinoma (P<0.05),but had no relation with the patients'age,pathological grade or lymph node metastasis(P>0.05). Conclusions:iNOS may act as an important tumor marker in lymph node metastasis of laryngeal carcinoma. It plays an important role in the invasion,metastasis and prognosis of laryngeal carcinoma.
2011, 36(12): 1309-1311.
Abstract:
Objective:To explore the efficiency and safety of endoscopic ligation by painless gastroscopy for treatment of esophageal varices. Methods:Twenty five patients with esophageal varices due to liver cirrhosis were performed 35 endoscopic ligations,10 cases of which received ordinary gastroscopy and 25 cases painless gastroscopy. The respiration,heart rate,blood pressure and blood oxygen saturation were observed during operation; and the patients' tolerance and the surgeon's satisfaction to the performance were investigated after the operation. The liver function and prothrombin time were examined before and 7 days after the operation in all the patients. Results:There were no significant differences with regard to the heart rate,respiration and oxygen saturation between the two groups(P>0.05),but the diastolic pressure and systolic pressure in the ordinary gastroscopy group were higher than those in the painless gastroscopy group during the operation(P<0.05). More satisfactory operating vision was obtained in the painless gastrocopy group than in the ordinary gastroscopy group (P<0.01). There were no significant differences with regard to the alanine aminotransferase, albumin,total bilirubin and prothrombin time before and seven days after the operation between the two groups(P>0.05). The alanine aminotransferase and prothrombin time at seven days after operation were significantly lower than those before operation in the painless gastroscopy group; the alanine aminotransferase at seven days after operation was significantly lower than that before operation in the ordinary gastroscopy group. Conclusions:Endoscopic esophageal varices ligation by painless gastroscopy is safe and easy to be accepted by patients and surgeons.
Objective:To explore the efficiency and safety of endoscopic ligation by painless gastroscopy for treatment of esophageal varices. Methods:Twenty five patients with esophageal varices due to liver cirrhosis were performed 35 endoscopic ligations,10 cases of which received ordinary gastroscopy and 25 cases painless gastroscopy. The respiration,heart rate,blood pressure and blood oxygen saturation were observed during operation; and the patients' tolerance and the surgeon's satisfaction to the performance were investigated after the operation. The liver function and prothrombin time were examined before and 7 days after the operation in all the patients. Results:There were no significant differences with regard to the heart rate,respiration and oxygen saturation between the two groups(P>0.05),but the diastolic pressure and systolic pressure in the ordinary gastroscopy group were higher than those in the painless gastroscopy group during the operation(P<0.05). More satisfactory operating vision was obtained in the painless gastrocopy group than in the ordinary gastroscopy group (P<0.01). There were no significant differences with regard to the alanine aminotransferase, albumin,total bilirubin and prothrombin time before and seven days after the operation between the two groups(P>0.05). The alanine aminotransferase and prothrombin time at seven days after operation were significantly lower than those before operation in the painless gastroscopy group; the alanine aminotransferase at seven days after operation was significantly lower than that before operation in the ordinary gastroscopy group. Conclusions:Endoscopic esophageal varices ligation by painless gastroscopy is safe and easy to be accepted by patients and surgeons.
2011, 36(12): 1312-1313,1316.
Abstract:
Objective:To investigate the clinical features and prognosis of clinically amyopathic dermatomyositis(CADM). Methods:The clinical data including the skin lesion,muscle involvement and pathological changes of the lung in 10 patients with CADM were analyzed retrospectively. Results:All the 10 patients presented characteristic skin lesions such as Gottron, S papulae (9 cases), heliotropic periorbital erythrema(8 cases), V-sign rash (10 cases) and shawl-sign rash (5 cases). Interstitial lung disease (ILD) occurred in 9 patients,and 4 of them died of respiratory failure resulting from rapidly progressive ILD. Conclusions:ILD is the most common and severe complication leading to mortality in CADM patients; therefore it should be highly alerted to and properly managed.
Objective:To investigate the clinical features and prognosis of clinically amyopathic dermatomyositis(CADM). Methods:The clinical data including the skin lesion,muscle involvement and pathological changes of the lung in 10 patients with CADM were analyzed retrospectively. Results:All the 10 patients presented characteristic skin lesions such as Gottron, S papulae (9 cases), heliotropic periorbital erythrema(8 cases), V-sign rash (10 cases) and shawl-sign rash (5 cases). Interstitial lung disease (ILD) occurred in 9 patients,and 4 of them died of respiratory failure resulting from rapidly progressive ILD. Conclusions:ILD is the most common and severe complication leading to mortality in CADM patients; therefore it should be highly alerted to and properly managed.
2011, 36(12): 1314-1316.
Abstract:
Objective:To study the value of serum calcium and C reaction protein (CRP) in predicting the severity of acute pancreatitis(AP). Methods:The serum calcium and CRP were monitored on day 1,2,3 and 4 in 52 patients with mild AP(MAP) and 21 patients with severe AP(SAP),their diagnostic value was assessed. Results:The level of serum CRP increased significantly in AP group on the the first day of admission and reached the peak on the second day,which was more obvious in SAP group than in MAP group(P<0.01). The diagnostic sensitivity of CRP was 87.3%,specificity 91.8% and AUC 0.924. No significant difference was found between the MAP group and the normal control(P>0.05),and the levels continued to desend with time goes by. Compared with the normal control group,the level of serum calcium in SAP group decreased notablely on the the first day of admission(P<0.01). The diagnostic sensitivity of serum calcium was 82.4%,specificity 86.1% and AUC 0.876. Conclusions:Serum calcium and CRP can be used as a reliable index in evaluation of the severity of acute pancreatitis.
Objective:To study the value of serum calcium and C reaction protein (CRP) in predicting the severity of acute pancreatitis(AP). Methods:The serum calcium and CRP were monitored on day 1,2,3 and 4 in 52 patients with mild AP(MAP) and 21 patients with severe AP(SAP),their diagnostic value was assessed. Results:The level of serum CRP increased significantly in AP group on the the first day of admission and reached the peak on the second day,which was more obvious in SAP group than in MAP group(P<0.01). The diagnostic sensitivity of CRP was 87.3%,specificity 91.8% and AUC 0.924. No significant difference was found between the MAP group and the normal control(P>0.05),and the levels continued to desend with time goes by. Compared with the normal control group,the level of serum calcium in SAP group decreased notablely on the the first day of admission(P<0.01). The diagnostic sensitivity of serum calcium was 82.4%,specificity 86.1% and AUC 0.876. Conclusions:Serum calcium and CRP can be used as a reliable index in evaluation of the severity of acute pancreatitis.
2011, 36(12): 1317-1319.
Abstract:
Objective:To explore the changes of bacterial spectrum and its resistance in pediatric sepsis so as to provide basis for correct use of antibiotics. Methods:The bacteria strains detected in the blood culture of children suffering from sepsis from 2005 to 2010 and the drug sensitivity were studied retrospectively. The patients admitted from 2005 to 2007 were included in Group A and patients admitted from 2008 to 2010 were in group B. The changes of bacterial spectrum and the drug resistance were compared between the two groups. Results:Five hundred and eighty-two strains were isolated,among which the Gram-positive bacteria accounted for 358 (61.5%),the Gram-negative bacteria 188 (32.3%),the Candida 8 (1.4%) and others 28 (4.8%). The major strains included coagulase-negative Staphylococci(51.2%),Escherichia coli(20.4%),Staphylococcus aureus(6.4%),Klebsiella pneumoniae(3.4%) and Enterobacter(3.4%). The drug resistance of group B to common antibiotics was higher than that of group A; meanwhile,the positive strains of extended-spectrum β-lactamases had a tendency to increase. Conclusions:The gram-positive bacterium is the main pathogen in pediatric septicemia and has a tendency to increase. With the application of broad-spectrum antibiotics,the drug resistance increases significantly.
Objective:To explore the changes of bacterial spectrum and its resistance in pediatric sepsis so as to provide basis for correct use of antibiotics. Methods:The bacteria strains detected in the blood culture of children suffering from sepsis from 2005 to 2010 and the drug sensitivity were studied retrospectively. The patients admitted from 2005 to 2007 were included in Group A and patients admitted from 2008 to 2010 were in group B. The changes of bacterial spectrum and the drug resistance were compared between the two groups. Results:Five hundred and eighty-two strains were isolated,among which the Gram-positive bacteria accounted for 358 (61.5%),the Gram-negative bacteria 188 (32.3%),the Candida 8 (1.4%) and others 28 (4.8%). The major strains included coagulase-negative Staphylococci(51.2%),Escherichia coli(20.4%),Staphylococcus aureus(6.4%),Klebsiella pneumoniae(3.4%) and Enterobacter(3.4%). The drug resistance of group B to common antibiotics was higher than that of group A; meanwhile,the positive strains of extended-spectrum β-lactamases had a tendency to increase. Conclusions:The gram-positive bacterium is the main pathogen in pediatric septicemia and has a tendency to increase. With the application of broad-spectrum antibiotics,the drug resistance increases significantly.
2011, 36(12): 1320-1321.
Abstract:
Objective:To evaluate the coronal approach in treatment of zygomatic fractures. Methods:The clinical data of 34 cases of zygomatic fracture which had been treated through semi-coronal incision and fixed internally with a mini-titanium plate in our hospital were analyzed retrospectively. Results:All the cases healed at one stage. No complication occurred after the operation. The facial contour and function recovered well. Conclusions:It is safe and effective to treat the zygomatic fracture by the approach of semi-coronal incision and internal fixation with a mini-titanium plate.
Objective:To evaluate the coronal approach in treatment of zygomatic fractures. Methods:The clinical data of 34 cases of zygomatic fracture which had been treated through semi-coronal incision and fixed internally with a mini-titanium plate in our hospital were analyzed retrospectively. Results:All the cases healed at one stage. No complication occurred after the operation. The facial contour and function recovered well. Conclusions:It is safe and effective to treat the zygomatic fracture by the approach of semi-coronal incision and internal fixation with a mini-titanium plate.
2011, 36(12): 1322-1324,1327.
Abstract:
Objective:To observe therapy effect of ω-3 polyunsaturated fatty acids in severe brain injury complicated by lung infection. Methods:Fifty-six cases of severe brain injury in patients with pulmonary infection were randomly divided into control group and Ewing group. Before division, all the patients were given the relevant examination and treatments such as antibiotics, reducing intracranial pressure,as well as endotracheal intubation, ventilator application and post-tracheotomy. Both groups were given energy supplement 105~125 kJ·kg-1·d-1,but in Ewing group,some of the heat were provided by the fat emulsion of ω-3 fatty acids. On the 0th,2nd,4th and 7th day patients'maximum day temperature,respiratory rate,heart rate,white blood cell,oxygenation index were monitored,and finally prognosis,ICU stay time,ventilation time and an average cost of the two groups of patients during ICU stay time were recorded. Results:The control group and Ewing group with positive anti-infection treatments are getting better. Clinical indicators of Ewing group and the control group on the 2nd day were not statistically significant(P>0.05). The vital signs(temperature,heart rate,respiration) and white blood cells of the two groups of patients showed downward trend over time,while the oxygenation index showed an upward trend over time,which is more significant for Ewing group on the 4th and 7th day compared with the control group, and the difference was statistically significant(P<0.05-P<0.01). After the 7-day observation in the prognosis outcome,the two groups showed no significant differences,not statistically significant(P>0.05). However,in the average ventilator time,ICU length of stay and cost,the two groups has significant differences,with statistical significance(P<0.01). Conclusions:ω-3 polyunsaturated fatty acids on severe brain injury in patients with pulmonary infection in the body has a protective effect.
Objective:To observe therapy effect of ω-3 polyunsaturated fatty acids in severe brain injury complicated by lung infection. Methods:Fifty-six cases of severe brain injury in patients with pulmonary infection were randomly divided into control group and Ewing group. Before division, all the patients were given the relevant examination and treatments such as antibiotics, reducing intracranial pressure,as well as endotracheal intubation, ventilator application and post-tracheotomy. Both groups were given energy supplement 105~125 kJ·kg-1·d-1,but in Ewing group,some of the heat were provided by the fat emulsion of ω-3 fatty acids. On the 0th,2nd,4th and 7th day patients'maximum day temperature,respiratory rate,heart rate,white blood cell,oxygenation index were monitored,and finally prognosis,ICU stay time,ventilation time and an average cost of the two groups of patients during ICU stay time were recorded. Results:The control group and Ewing group with positive anti-infection treatments are getting better. Clinical indicators of Ewing group and the control group on the 2nd day were not statistically significant(P>0.05). The vital signs(temperature,heart rate,respiration) and white blood cells of the two groups of patients showed downward trend over time,while the oxygenation index showed an upward trend over time,which is more significant for Ewing group on the 4th and 7th day compared with the control group, and the difference was statistically significant(P<0.05-P<0.01). After the 7-day observation in the prognosis outcome,the two groups showed no significant differences,not statistically significant(P>0.05). However,in the average ventilator time,ICU length of stay and cost,the two groups has significant differences,with statistical significance(P<0.01). Conclusions:ω-3 polyunsaturated fatty acids on severe brain injury in patients with pulmonary infection in the body has a protective effect.
2011, 36(12): 1325-1327.
Abstract:
Objective:To compare the effects and side effects of EOF (oxaliplatin combined with epirubicin and 5-Fluorouacil/leucovorin) with XELOX(oxaliplatin combined with xeloda) in treatment of patients with advanced gastric cancer. Methods:Among 75 patients with advanced gastric cancer,39 cases received EOF treatment and 36 cases received XELOX treatment. The efficacy and toxicity were evaluated after 2 therapy courses. Every therapy course included 3 weeks. Results:The overall effectve rate was 43.6% in EOF group,and 41.7% in XELOX group. There was no significant difference between the two groups(P>0.05). The incidence of hand-foot syndrome in XELOX group was higher than that in EOF group(P<0.01),but that of myelosuppression,nausea,vomiting and alopecie in EOX group higher than that in XEL0X group(P<0.01). Conclusions:The treatment of EOF and XELOX shows well-confirmed effects for advanced gastric cancer. XELOX is more convenient and suitable for the elder or weaker patients,but EOF is more suitable for the recurrent patients.
Objective:To compare the effects and side effects of EOF (oxaliplatin combined with epirubicin and 5-Fluorouacil/leucovorin) with XELOX(oxaliplatin combined with xeloda) in treatment of patients with advanced gastric cancer. Methods:Among 75 patients with advanced gastric cancer,39 cases received EOF treatment and 36 cases received XELOX treatment. The efficacy and toxicity were evaluated after 2 therapy courses. Every therapy course included 3 weeks. Results:The overall effectve rate was 43.6% in EOF group,and 41.7% in XELOX group. There was no significant difference between the two groups(P>0.05). The incidence of hand-foot syndrome in XELOX group was higher than that in EOF group(P<0.01),but that of myelosuppression,nausea,vomiting and alopecie in EOX group higher than that in XEL0X group(P<0.01). Conclusions:The treatment of EOF and XELOX shows well-confirmed effects for advanced gastric cancer. XELOX is more convenient and suitable for the elder or weaker patients,but EOF is more suitable for the recurrent patients.
2011, 36(12): 1328-1329,1332.
Abstract:
Objective:To investigate the effect and clinical applicable value of percutaneous sclerotherapy guided by ultrasound for lymphatic malformations. Methods:Ten cases of lymphatic malformations were treated with percutaneous sclerosis therapy guided by ultrasound,which the style,location and size were identified by ultrasonography and magnetic resonance imaging. Results:The lesion in 8 patients were complete disappearance,2 were obviously improved,which required a second of third therapy,the lesion were complete disappearance too. No allergy,no local ulceration and pulmonary fibrosis complications. Conclusions:Percutaneous sclerotherapy guided by ultrasound is accurate, minimally invasive, safe, and reliable for lymphatic malformations in body surface, which may avoid or decrease the happening of complications.
Objective:To investigate the effect and clinical applicable value of percutaneous sclerotherapy guided by ultrasound for lymphatic malformations. Methods:Ten cases of lymphatic malformations were treated with percutaneous sclerosis therapy guided by ultrasound,which the style,location and size were identified by ultrasonography and magnetic resonance imaging. Results:The lesion in 8 patients were complete disappearance,2 were obviously improved,which required a second of third therapy,the lesion were complete disappearance too. No allergy,no local ulceration and pulmonary fibrosis complications. Conclusions:Percutaneous sclerotherapy guided by ultrasound is accurate, minimally invasive, safe, and reliable for lymphatic malformations in body surface, which may avoid or decrease the happening of complications.
2011, 36(12): 1330-1332.
Abstract:
Objective:To investigate the clinical effects of holmium laser lithotripsy through ureteropyeloscopy on patient with ureteral calculi. Methods:The data of 86 cases underwent holmium laser lithotripsy through ureteropyeloscopy were analysed retrospectively. Gralet rate, lithagogue rate and complication were statistically analysed. Results:Among 86 cases, gralet rate was 82 cases in a single procedure. Ureteral stone moved up into the pelvis in 3 cases, a double J tube was placed in the renal pelvis and extrocorporeal shock wave lithotripsy was used. A case with serious narrow distal ureters was conducted open operation. The mean operative time was (42.3±7.4) min and the mean hospital stay was (2.5±1.7) days. The duration of follow-up was 1 to 3 months for all patients. The stone free rate was 98.8% (85 of 86). Ureteral perforation and postoperative hyperpyrexia happened in 1 case and 6 cases respectively. Conclusions:The method of holmium laser lithotripsy through ureteropyeloscopy is safety, reliability and less invasion, so it is the best way to cure the middle and distal ureter calculi.
Objective:To investigate the clinical effects of holmium laser lithotripsy through ureteropyeloscopy on patient with ureteral calculi. Methods:The data of 86 cases underwent holmium laser lithotripsy through ureteropyeloscopy were analysed retrospectively. Gralet rate, lithagogue rate and complication were statistically analysed. Results:Among 86 cases, gralet rate was 82 cases in a single procedure. Ureteral stone moved up into the pelvis in 3 cases, a double J tube was placed in the renal pelvis and extrocorporeal shock wave lithotripsy was used. A case with serious narrow distal ureters was conducted open operation. The mean operative time was (42.3±7.4) min and the mean hospital stay was (2.5±1.7) days. The duration of follow-up was 1 to 3 months for all patients. The stone free rate was 98.8% (85 of 86). Ureteral perforation and postoperative hyperpyrexia happened in 1 case and 6 cases respectively. Conclusions:The method of holmium laser lithotripsy through ureteropyeloscopy is safety, reliability and less invasion, so it is the best way to cure the middle and distal ureter calculi.
2011, 36(12): 1333-1335.
Abstract:
Objective:To explore the risk factors and preventive measures for conversion to open cholecystectomy in emergent laparoscopic cholecystectomy(LC). Methods:The clinical data of 103 cases of LC were performed single factor analysis and multiple factor analysis with logistic regression; and the independent risk factors affecting the conversion of LC to laparotomy were concluded. Results:The operation was successfully performed in 103 cases,and 9 cases were converted to open surgery. The analysis revealed that the risk factors included thickened gallbladder wall,times of cholecystitis attacks, history of upper abdominal operation, cystic duct calculus incarceration and ALT(P<0.05 to P<0.01). Conclusions:The risk factors of conversion to open cholecystectomy include thickened gallbladder wall, history of upper abdominal operation,times of acute cholecystitis attacks(> 3 times), stone incarceration on the neck of gallbladder and ALT levels. Analysis of the risk factors and master the operative skills is efective method to reduce the rate of complications and conversion to laparotomy.
Objective:To explore the risk factors and preventive measures for conversion to open cholecystectomy in emergent laparoscopic cholecystectomy(LC). Methods:The clinical data of 103 cases of LC were performed single factor analysis and multiple factor analysis with logistic regression; and the independent risk factors affecting the conversion of LC to laparotomy were concluded. Results:The operation was successfully performed in 103 cases,and 9 cases were converted to open surgery. The analysis revealed that the risk factors included thickened gallbladder wall,times of cholecystitis attacks, history of upper abdominal operation, cystic duct calculus incarceration and ALT(P<0.05 to P<0.01). Conclusions:The risk factors of conversion to open cholecystectomy include thickened gallbladder wall, history of upper abdominal operation,times of acute cholecystitis attacks(> 3 times), stone incarceration on the neck of gallbladder and ALT levels. Analysis of the risk factors and master the operative skills is efective method to reduce the rate of complications and conversion to laparotomy.
2011, 36(12): 1336-1338.
Abstract:
Objective:To investigate the diagnostic significance of C-reactive protein (CRP) and procalcitonin (PCT) on sepsis. Methods:According to the criterion of sepsis compiled by America College of Chest Physicians/America Society of Critic Care Medicine in 1991. Sixty-four severe patients were selected in ICU. The patients were divided into two groups:sepsis group(n=50) and none sepsis group(n=14),the baseline clinical data of two groups were comparable. PCT and CRP were measured by double-antibody sandwich ELISA method, solid-phase semi-quantitative immunochromatography and quantitative immunization rate turbidimetry, the sensitivity,specificity,positive predictive value,negative predictive value,Youdens' index of two biomarkers were calculated. The ROC was described for further analysis. Results:The sensitivity, specificity, positive predictive value, negative predictive value, Youdens' index and the area under the curve of ROC of PCT were better than those of CRP. And the combination of two biomarkers had higher sensitivity than that of CRP or PCT separately. Conclusions:PCT has a relatively good diagnostic significance on sepsis. The combination of multibiomarkers should be considered to apply in order to increase the sensitivity of dignosis for sepsis.
Objective:To investigate the diagnostic significance of C-reactive protein (CRP) and procalcitonin (PCT) on sepsis. Methods:According to the criterion of sepsis compiled by America College of Chest Physicians/America Society of Critic Care Medicine in 1991. Sixty-four severe patients were selected in ICU. The patients were divided into two groups:sepsis group(n=50) and none sepsis group(n=14),the baseline clinical data of two groups were comparable. PCT and CRP were measured by double-antibody sandwich ELISA method, solid-phase semi-quantitative immunochromatography and quantitative immunization rate turbidimetry, the sensitivity,specificity,positive predictive value,negative predictive value,Youdens' index of two biomarkers were calculated. The ROC was described for further analysis. Results:The sensitivity, specificity, positive predictive value, negative predictive value, Youdens' index and the area under the curve of ROC of PCT were better than those of CRP. And the combination of two biomarkers had higher sensitivity than that of CRP or PCT separately. Conclusions:PCT has a relatively good diagnostic significance on sepsis. The combination of multibiomarkers should be considered to apply in order to increase the sensitivity of dignosis for sepsis.
2011, 36(12): 1339-1340.
Abstract:
Objective:To investigate the effect of low-dose of erythromycin on feeding intolerance in premature infants. Methods:Seventy-four premature infants with feeding intolerance were randomly divided into treatment group (36 cases) and control group (38 cases). The control group received routine therapy, while the treatment group were administered intravenous injection of erythromycin 5 mg·kg-1·d-1 once a day for 5-7 days in addition to the routine therapy. Results:The therapeutic effect of treatment group was superior to the control group in the extinction time of vomiting, stomach retention and abdominal distension as well as the time of nutrition getting full into the gastrointestinal tract and achieving birth weight(P<0.05). Diarrhea occurred in 2 cases in the treatment group and 1 case in the control group. There wasn't statistical significance(P>0.05). Conclusions:The erythromycin in lowdose can improve the feeding into intolerance of premature children. It is worthly of application.
Objective:To investigate the effect of low-dose of erythromycin on feeding intolerance in premature infants. Methods:Seventy-four premature infants with feeding intolerance were randomly divided into treatment group (36 cases) and control group (38 cases). The control group received routine therapy, while the treatment group were administered intravenous injection of erythromycin 5 mg·kg-1·d-1 once a day for 5-7 days in addition to the routine therapy. Results:The therapeutic effect of treatment group was superior to the control group in the extinction time of vomiting, stomach retention and abdominal distension as well as the time of nutrition getting full into the gastrointestinal tract and achieving birth weight(P<0.05). Diarrhea occurred in 2 cases in the treatment group and 1 case in the control group. There wasn't statistical significance(P>0.05). Conclusions:The erythromycin in lowdose can improve the feeding into intolerance of premature children. It is worthly of application.
2011, 36(12): 1341-1342,1345.
Abstract:
Objective:To study the relationship of serum visfatin levels and acute coronary syndrome(ACS) and the role of serum visfatin in vulnerable plaques. Methods:The serum levels of visfatin and matrix metalloproteinase-9(MMP-9) in 40 cases with ACS,22 cases with stable angina pectoris(SAP) and 26 control cases were measured by ELISA,and the correlation between visfatin and MMP-9 was evaluated. Results:The levels of visfatin and MMP-9 in cases with ACS were significantly higher than those in SAP and control groups(P<0.01). Significant positive correlation of visfatin and MMP-9 in patients with ACS were found(P<0.01). Conclusions:The serum levels of visfatin and ACS exist correlation. Visfatin may act as the biomarkers of vulnerable plaques and be applied in the early prevention and management of ACS.
Objective:To study the relationship of serum visfatin levels and acute coronary syndrome(ACS) and the role of serum visfatin in vulnerable plaques. Methods:The serum levels of visfatin and matrix metalloproteinase-9(MMP-9) in 40 cases with ACS,22 cases with stable angina pectoris(SAP) and 26 control cases were measured by ELISA,and the correlation between visfatin and MMP-9 was evaluated. Results:The levels of visfatin and MMP-9 in cases with ACS were significantly higher than those in SAP and control groups(P<0.01). Significant positive correlation of visfatin and MMP-9 in patients with ACS were found(P<0.01). Conclusions:The serum levels of visfatin and ACS exist correlation. Visfatin may act as the biomarkers of vulnerable plaques and be applied in the early prevention and management of ACS.
2011, 36(12): 1343-1345.
Abstract:
Objective:To investigate the reltationship between the expression of heparanase and syndecan-1 gene mRNA and the pathological grade in human gliomas. Methods:The realtime polymerase chain reactions was empolyed to detect the expression level of heparanase and syndecan-1 gene mRNA in 40 cases of human gliomas and in 10 cases of benign brain tumors,which was used to evaluate tumor grade. Results:The expression level of heparanase mRNA in gliomas is higher than that in brain tumors(P<0.01),and rising with tumor grade. The expression level of syndecan-1 mRNA in gliomas is significant lower compared to that of benign brain tumors(P<0.01),and decresing with tumor grade. The expression level of heparanase mRNA is reversely correlated to the expression level of syndecan-1 mRNA in human gliomas(P<0.01). Conclusions:The heparanase and syndecan-1 gene expression maybe correlate to the oncogensis and progression of human gliomas,which can provide an effective way to diagnose and treat the human gliomas.
Objective:To investigate the reltationship between the expression of heparanase and syndecan-1 gene mRNA and the pathological grade in human gliomas. Methods:The realtime polymerase chain reactions was empolyed to detect the expression level of heparanase and syndecan-1 gene mRNA in 40 cases of human gliomas and in 10 cases of benign brain tumors,which was used to evaluate tumor grade. Results:The expression level of heparanase mRNA in gliomas is higher than that in brain tumors(P<0.01),and rising with tumor grade. The expression level of syndecan-1 mRNA in gliomas is significant lower compared to that of benign brain tumors(P<0.01),and decresing with tumor grade. The expression level of heparanase mRNA is reversely correlated to the expression level of syndecan-1 mRNA in human gliomas(P<0.01). Conclusions:The heparanase and syndecan-1 gene expression maybe correlate to the oncogensis and progression of human gliomas,which can provide an effective way to diagnose and treat the human gliomas.
2011, 36(12): 1346-1347.
Abstract:
Objective:To observe the effect of ransumbilical single-port access in laparoscopic cholecystectomy(LC),and to sum up the surgical experience. Methods:The clinical data of 20 cases having received transumbilical single-hole LC were reviewed and analyzed. Among the 20 cases,11 suffered from cholecystitis and biliary calculus, 7 cholecystitis and polyp of the gallbladder, 2 cholecystitis and biliary calculus and polyp of the gallbladder. Results:The operation was successful in all the 20 cases. No infection or cholerrhagia occurred. All the patients were discharged from hospital 4-7 days after operation. No obvious scar was observed in the umbilical region during the subsequent visits one month later. Conclusions:As a new technology,transumbilical single-hole LC can achieve the effect of both minimal invasion and good vision. It is safe and well worth popularizing.
Objective:To observe the effect of ransumbilical single-port access in laparoscopic cholecystectomy(LC),and to sum up the surgical experience. Methods:The clinical data of 20 cases having received transumbilical single-hole LC were reviewed and analyzed. Among the 20 cases,11 suffered from cholecystitis and biliary calculus, 7 cholecystitis and polyp of the gallbladder, 2 cholecystitis and biliary calculus and polyp of the gallbladder. Results:The operation was successful in all the 20 cases. No infection or cholerrhagia occurred. All the patients were discharged from hospital 4-7 days after operation. No obvious scar was observed in the umbilical region during the subsequent visits one month later. Conclusions:As a new technology,transumbilical single-hole LC can achieve the effect of both minimal invasion and good vision. It is safe and well worth popularizing.
2011, 36(12): 1348-1349,1352.
Abstract:
Objective:To explore the effect of thoracic epidural block on central venous oxygen saturation(ScVO2) in elderly patients undergoing chest surgery. Methods:Forty ASA Ⅰ-Ⅱ patients undergoing esophagectomy or lobectomy were randomized to receive either general combined thoracic epidural anesthesia(group A,n=20) or pure general anesthesia (group B,n=20). In group A, thoracic 5-6 or 6-7(T5-6 or T6-7) vertebral space for puncture and epidural catheter was placed before general anesthesia. A test dose of 4 ml 1.5% lidocaine was given. When correct positioning of the epidural catheter was confirmed,general anesthesia in the two groups was induced with etomidate 0.2-0.3 mg/kg,fentanyl 3-4 μg/kg and syn-atracurium 0.15 mg/kg,anesthesia was maintained with 1%-3% sevoflurane and remifentanil and intermittent intravenous injection bolused of syn-atracurium. One point five percent lidocaine was continuously infused at a rate of 5-6 ml/h during operation in group A. Patients in group B were received only general anesthesia. The hemodynamics,SaO2 and ScVO2 in two groups pre-,intra- and after-operation were recorded. Results:Compared with group B,the hemodynamics in group A was stable. ScVO2 in group A and group B was dropped significantly after operation(P<0.01), but the ScVO2 in group B was droped furtherly. Conclusions:Thoracic epidural block can significantly raise ScVO2 and depress the consumption of oxygen in patients who received chest surgery.
Objective:To explore the effect of thoracic epidural block on central venous oxygen saturation(ScVO2) in elderly patients undergoing chest surgery. Methods:Forty ASA Ⅰ-Ⅱ patients undergoing esophagectomy or lobectomy were randomized to receive either general combined thoracic epidural anesthesia(group A,n=20) or pure general anesthesia (group B,n=20). In group A, thoracic 5-6 or 6-7(T5-6 or T6-7) vertebral space for puncture and epidural catheter was placed before general anesthesia. A test dose of 4 ml 1.5% lidocaine was given. When correct positioning of the epidural catheter was confirmed,general anesthesia in the two groups was induced with etomidate 0.2-0.3 mg/kg,fentanyl 3-4 μg/kg and syn-atracurium 0.15 mg/kg,anesthesia was maintained with 1%-3% sevoflurane and remifentanil and intermittent intravenous injection bolused of syn-atracurium. One point five percent lidocaine was continuously infused at a rate of 5-6 ml/h during operation in group A. Patients in group B were received only general anesthesia. The hemodynamics,SaO2 and ScVO2 in two groups pre-,intra- and after-operation were recorded. Results:Compared with group B,the hemodynamics in group A was stable. ScVO2 in group A and group B was dropped significantly after operation(P<0.01), but the ScVO2 in group B was droped furtherly. Conclusions:Thoracic epidural block can significantly raise ScVO2 and depress the consumption of oxygen in patients who received chest surgery.
2011, 36(12): 1350-1352.
Abstract:
Objective:To assess the clinical value of dual-source computed tomography coronary angiography(DSCTA) in diagnosing coronary heant disease(CHD) patients with multi vessel disease and type 2 diabetes. Methods:Forty-eight patients suffering from coronary artery disease and type 2 diabetes were underwent both DSCTA and coronary angiography (CAG). Regarding CAG as a standard reference,50% stenosis in two or more than two major coronary arteries were as multi vessel disease,the reliability of DSCTA for diagnosing coronary stenosis was analyzed. Results:In the forty-eight patients,compared with CAG,the sensitivity and specificity of DSCTA for detecting light, middle and severe multivessel disease were 91.91%, 100.00%, 100.00%, 94.72%, respectively. Conclusions:DSCTA could reliably provide promising value of diagnosing multi vessel disease in CHD patients with type 2 diabetes.
Objective:To assess the clinical value of dual-source computed tomography coronary angiography(DSCTA) in diagnosing coronary heant disease(CHD) patients with multi vessel disease and type 2 diabetes. Methods:Forty-eight patients suffering from coronary artery disease and type 2 diabetes were underwent both DSCTA and coronary angiography (CAG). Regarding CAG as a standard reference,50% stenosis in two or more than two major coronary arteries were as multi vessel disease,the reliability of DSCTA for diagnosing coronary stenosis was analyzed. Results:In the forty-eight patients,compared with CAG,the sensitivity and specificity of DSCTA for detecting light, middle and severe multivessel disease were 91.91%, 100.00%, 100.00%, 94.72%, respectively. Conclusions:DSCTA could reliably provide promising value of diagnosing multi vessel disease in CHD patients with type 2 diabetes.
2011, 36(12): 1353-1354.
Abstract:
Objective:To summarize the experience on treatment of restoring residual root and crown of anterior and posterior teeth by post and core crown. Methods:The residual root and crown of 126 anterior and posterior teeth were received root canal therapy firstly, then the anterior teeth were adopted casting post and core or finished products root thread post,resin core,while the posterior teeth were adopted finished products root thread post, resin core, or porcelain-fused-to-metal or metal crown. Results:The 126 posterior and anterior teeth were reexamined after 12 months,120 teeth were achieved the success standard,6 teeth was failed. Conclusions:The residual root and crown of anterior and posterior teeth without significant periodontal destruction can be absolutely kept and maintained normal mastication function,furtherly can meet the requirement of beauty,when the anterior and posterior teeth were adopted casting post and core,finished products root thread post,resin core or porcelain-fused-to-metal or metal crown therapy.
Objective:To summarize the experience on treatment of restoring residual root and crown of anterior and posterior teeth by post and core crown. Methods:The residual root and crown of 126 anterior and posterior teeth were received root canal therapy firstly, then the anterior teeth were adopted casting post and core or finished products root thread post,resin core,while the posterior teeth were adopted finished products root thread post, resin core, or porcelain-fused-to-metal or metal crown. Results:The 126 posterior and anterior teeth were reexamined after 12 months,120 teeth were achieved the success standard,6 teeth was failed. Conclusions:The residual root and crown of anterior and posterior teeth without significant periodontal destruction can be absolutely kept and maintained normal mastication function,furtherly can meet the requirement of beauty,when the anterior and posterior teeth were adopted casting post and core,finished products root thread post,resin core or porcelain-fused-to-metal or metal crown therapy.
2011, 36(12): 1355-1356.
Abstract:
Objective:To explore the reasons for misdiagnosis of anxiety disorders as coronary heart disease. Methods:Thirty cases with anxiety disorders misdiagnosed as coronary heart disease were analyzed retrospectively. Results:The patients were relatively young at age(30 to 45); female were more easily affected,accounting for 1.5 times more than the male; most of the victims endured great social and mental pressure. The mark of HAMA after treatment was obviously lower than that before treatment(P<0.01). Six cases recovered completely,18 improved significantly,5 improved and 1 had no response. Conclusions:As anxiety disorders and coronary heart disease present many similar or the same symptoms,it is easy to be misdiagnosed. Besides possessing rich professional knowledge,clinical doctors should ask about the case history of the patients in detail and make comprehensive analysis to decrease the incidence of misdiagnosis.
Objective:To explore the reasons for misdiagnosis of anxiety disorders as coronary heart disease. Methods:Thirty cases with anxiety disorders misdiagnosed as coronary heart disease were analyzed retrospectively. Results:The patients were relatively young at age(30 to 45); female were more easily affected,accounting for 1.5 times more than the male; most of the victims endured great social and mental pressure. The mark of HAMA after treatment was obviously lower than that before treatment(P<0.01). Six cases recovered completely,18 improved significantly,5 improved and 1 had no response. Conclusions:As anxiety disorders and coronary heart disease present many similar or the same symptoms,it is easy to be misdiagnosed. Besides possessing rich professional knowledge,clinical doctors should ask about the case history of the patients in detail and make comprehensive analysis to decrease the incidence of misdiagnosis.
2011, 36(12): 1357-1358,1361.
Abstract:
Objective:To observe the change of high sensitivity C-reactive protein(hs-CRP) in patients with atrial fibrillation so as to explore the correlation between inflammation and atrial fibrillation. Methods:Eighty-four patients with atrial fibrillation were divided into two groups:persistent atrial fibrillation(> 30 days) group(43 cases) and paroxysmal atrial fibrillation(< 48 hours) group(41 cases),and 40 patients with sinus rhythm acted as control. The serum hs-CRP content of each group was measured by enzyme linked immunosorbent assay. Results:The serum level of hs-CRP in the persistent atrial fibrillation group was higher than that in the paroxysmal atrial fibrillation group and the control group (P<0.01); the serum level of hs-CRP content in the paroxysmal atrial fibrillation group was higher than that in the control group(P<0.01). Conclusions:The result indicates that inflammation may play an important role in the genesis and sustaining of atrial fibrillation.
Objective:To observe the change of high sensitivity C-reactive protein(hs-CRP) in patients with atrial fibrillation so as to explore the correlation between inflammation and atrial fibrillation. Methods:Eighty-four patients with atrial fibrillation were divided into two groups:persistent atrial fibrillation(> 30 days) group(43 cases) and paroxysmal atrial fibrillation(< 48 hours) group(41 cases),and 40 patients with sinus rhythm acted as control. The serum hs-CRP content of each group was measured by enzyme linked immunosorbent assay. Results:The serum level of hs-CRP in the persistent atrial fibrillation group was higher than that in the paroxysmal atrial fibrillation group and the control group (P<0.01); the serum level of hs-CRP content in the paroxysmal atrial fibrillation group was higher than that in the control group(P<0.01). Conclusions:The result indicates that inflammation may play an important role in the genesis and sustaining of atrial fibrillation.
2011, 36(12): 1359-1361.
Abstract:
Objective:To explore the clinical value of mammography and stereotactic biopsy in nonpalpable breast lesions. Methods:Stereotactic biopsy was performed in 85 patients who appeared clinical nonpalpable breast lesions,but abnormality by mammography. The results were analyzed retrospectively. Results:All lesions were resected only one time. Guide metal wire was completely taken out. There was no complication. In 85 cases,62 cases were diagnosed to be benign lesion,the other 23 cases were finally diagnosed to be malignant. Conclusions:Mammography and stereotactic biopsy are useful in the diagnosis of the clinical nonpapable breast lesions. It is safe and reliable,which is an important method to find early breast cancer.
Objective:To explore the clinical value of mammography and stereotactic biopsy in nonpalpable breast lesions. Methods:Stereotactic biopsy was performed in 85 patients who appeared clinical nonpalpable breast lesions,but abnormality by mammography. The results were analyzed retrospectively. Results:All lesions were resected only one time. Guide metal wire was completely taken out. There was no complication. In 85 cases,62 cases were diagnosed to be benign lesion,the other 23 cases were finally diagnosed to be malignant. Conclusions:Mammography and stereotactic biopsy are useful in the diagnosis of the clinical nonpapable breast lesions. It is safe and reliable,which is an important method to find early breast cancer.
2011, 36(12): 1362-1364.
Abstract:
Objective:To explore the effect of immunoglobulin(IG) on patients with end stage liver diseases combined with infection. Methods:One hundred and five patients with end stage liver diseases combined with infection were randomized into treatment group (53 cases) and control group (52 cases). The control group received the routine therapy of antibiotics and the treatment group were administered IG in addition to the antibiotics. The clinical effect was evaluated according to the Child-Pugh score. Results:The patients with the score of Child-Pugh < 10 in the two groups presented increased total bilirubin,decreased cholesterol and prolonged prothrombin time after one week's therapy, and there were no significant differences between the two groups. In patients with the score of ChildPugh 10-15, the percentage of efficiency was 80.00% and 46.15% (P<0.05) in the study group and the control group respectively,and the percentage of inefficiency was 20.00% and 53.85% (P<0.05). Conclusions:Early therapy of IG can effectively increase the anti-infection ability of patients with end stage liver diseases combined with infection whose score of Child-Pugh is 10-15, and may help to reduce the mycotic double infection.
Objective:To explore the effect of immunoglobulin(IG) on patients with end stage liver diseases combined with infection. Methods:One hundred and five patients with end stage liver diseases combined with infection were randomized into treatment group (53 cases) and control group (52 cases). The control group received the routine therapy of antibiotics and the treatment group were administered IG in addition to the antibiotics. The clinical effect was evaluated according to the Child-Pugh score. Results:The patients with the score of Child-Pugh < 10 in the two groups presented increased total bilirubin,decreased cholesterol and prolonged prothrombin time after one week's therapy, and there were no significant differences between the two groups. In patients with the score of ChildPugh 10-15, the percentage of efficiency was 80.00% and 46.15% (P<0.05) in the study group and the control group respectively,and the percentage of inefficiency was 20.00% and 53.85% (P<0.05). Conclusions:Early therapy of IG can effectively increase the anti-infection ability of patients with end stage liver diseases combined with infection whose score of Child-Pugh is 10-15, and may help to reduce the mycotic double infection.
2011, 36(12): 1365-1366.
Abstract:
Objective:To explore the method of diagnosing atypicai placental abruption so as to enhance the diagnosis level. Methods:The clinical data of 48 patients with atypical placental abruption were analyzed retrospectively. Results:The atypical placental abruption accounted for 1.6% of all the deliveries at the corresponding time period. The diagnosis mainly relied on the clinical monitoring data and clinical signs. The accuracy diagnosis reached 91.7%. Conclusions:The diagnosis of atypical placental abruption should be based on the history,clinical symptoms,B-test,fetal heart rate monitoring and dynamic observation.
Objective:To explore the method of diagnosing atypicai placental abruption so as to enhance the diagnosis level. Methods:The clinical data of 48 patients with atypical placental abruption were analyzed retrospectively. Results:The atypical placental abruption accounted for 1.6% of all the deliveries at the corresponding time period. The diagnosis mainly relied on the clinical monitoring data and clinical signs. The accuracy diagnosis reached 91.7%. Conclusions:The diagnosis of atypical placental abruption should be based on the history,clinical symptoms,B-test,fetal heart rate monitoring and dynamic observation.
2011, 36(12): 1373-1376,1379.
Abstract:
Objective:To study the relationship between the expressions of hepatitis B virus (HBV) antigen and CD8+CD28+T lymphocyte in liver tissue of hepatitis B patients and analyze the corresponding clinical significance. Methods:The expressions of HBV antigens and nucleic acid were detected by immunohistochemistry and situ hybridization method in each groups(mild,moderate and severe hepatitis B groups). The expression of CD28 T lymphocyte in liver tissue was determined by immunohistochemistry and the results of each group were compared. Results:There was no significant difference in the expressions of HBsAg(+) HBcAg(+), HBsAg(+) HBcAg(-),HBsAg(-) HBcAg(+) and HBsAg(-) HBcAg(-) in the three groups(P>0.05). The expression of CD8+CD28+T lymphocyte in mild hepatitis group was lower than in control group,the expressions of CD8+CD28+T lymphocyte and CD8+CD28-T lymphocyte were higher in moderate and severe hepatitis B groups than in control group(P<0.05). The expression of CD8+CD28+T lymphocyte was increased progressively,while the expression of CD8+CD28-T lymphocyte was decreased progressively with the grade of inflammation(P<0.01). The expression of CD8+CD28+T lymphocyte in moderate and severe hepatitis patients with HBsAg(+) HBcAg(+) was lower than in other groups with HBsAg(+) HBcAg(-),HBsAg(-) HBcAg(+) and HBsAg(-) HBcAg(-) (P<0.05~P<0.01). There were no significant difference about the expressions of HBV DNA and HBeAg in different levels of inflammation(P>0.05). Conclusions:HBV can reflect the replication of HBV DNA in liver tissue more accurately. As an indirect reflection of immune status, it can be used as a selector of anti-viral treatment indication and a predictor of efficacy. The expression of CD28 on the surface of CD8+T lymphocyte is closely related with the clearance of HBV,and the high expression of CD28 can promote HBV clearance in liver cells.
Objective:To study the relationship between the expressions of hepatitis B virus (HBV) antigen and CD8+CD28+T lymphocyte in liver tissue of hepatitis B patients and analyze the corresponding clinical significance. Methods:The expressions of HBV antigens and nucleic acid were detected by immunohistochemistry and situ hybridization method in each groups(mild,moderate and severe hepatitis B groups). The expression of CD28 T lymphocyte in liver tissue was determined by immunohistochemistry and the results of each group were compared. Results:There was no significant difference in the expressions of HBsAg(+) HBcAg(+), HBsAg(+) HBcAg(-),HBsAg(-) HBcAg(+) and HBsAg(-) HBcAg(-) in the three groups(P>0.05). The expression of CD8+CD28+T lymphocyte in mild hepatitis group was lower than in control group,the expressions of CD8+CD28+T lymphocyte and CD8+CD28-T lymphocyte were higher in moderate and severe hepatitis B groups than in control group(P<0.05). The expression of CD8+CD28+T lymphocyte was increased progressively,while the expression of CD8+CD28-T lymphocyte was decreased progressively with the grade of inflammation(P<0.01). The expression of CD8+CD28+T lymphocyte in moderate and severe hepatitis patients with HBsAg(+) HBcAg(+) was lower than in other groups with HBsAg(+) HBcAg(-),HBsAg(-) HBcAg(+) and HBsAg(-) HBcAg(-) (P<0.05~P<0.01). There were no significant difference about the expressions of HBV DNA and HBeAg in different levels of inflammation(P>0.05). Conclusions:HBV can reflect the replication of HBV DNA in liver tissue more accurately. As an indirect reflection of immune status, it can be used as a selector of anti-viral treatment indication and a predictor of efficacy. The expression of CD28 on the surface of CD8+T lymphocyte is closely related with the clearance of HBV,and the high expression of CD28 can promote HBV clearance in liver cells.
2011, 36(12): 1377-1379.
Abstract:
Objective:To investigate the effect of blood storage time on the results of automatic blood cell analyzer for normal and pathological specimens at room temperature. Methods:The blood samples of 40 healthy persons and 20 hematologic patients were collected to be analyzed in 20 minutes. Then,the remnant blood samples were analyzed respectively after being stored for 8,9,10,11,12 and 24 hours at room temperature(25±0.5)℃,and the results were compared with thats in 20 minutes as controls. Results:The result of platelet in 40 blood samples were decreased obviously at 11th hour,and had significant differences with controls(P<0.05). At 24th hour,the percentage of neutrophils was decreased while the ratio of lymphocytes and monocytes was increased obviously(P<0.05). The results of red blood cells and platelet from pathological specimens were significantly lower than controls(P<0.05). Conclusions:The repeatability and the level of preanalytical quality control could be enhanced when the samples were analyzed within 10 hours at room temperature.
Objective:To investigate the effect of blood storage time on the results of automatic blood cell analyzer for normal and pathological specimens at room temperature. Methods:The blood samples of 40 healthy persons and 20 hematologic patients were collected to be analyzed in 20 minutes. Then,the remnant blood samples were analyzed respectively after being stored for 8,9,10,11,12 and 24 hours at room temperature(25±0.5)℃,and the results were compared with thats in 20 minutes as controls. Results:The result of platelet in 40 blood samples were decreased obviously at 11th hour,and had significant differences with controls(P<0.05). At 24th hour,the percentage of neutrophils was decreased while the ratio of lymphocytes and monocytes was increased obviously(P<0.05). The results of red blood cells and platelet from pathological specimens were significantly lower than controls(P<0.05). Conclusions:The repeatability and the level of preanalytical quality control could be enhanced when the samples were analyzed within 10 hours at room temperature.
2011, 36(12): 1380-1381,1384.
Abstract:
Objective:To survey the serotype and antibiotics resistance of enteropathogenic Escherichia coli(E.coli) in children with diarrhea in Nanjing area. Methods:Five thousand four hundred and nine cases of feces samples were cultured,isolated E.coli strains were indentified by serotyping,and the antibiotic susceptibility was determined by disk diffusion test. Results:Six hundred and thirtythree strains of E.coli were isolated(11.70%) and 12 serotypes were detected. The incidence of ESBLs was 82.15% in the isolated strains. Conclusions:E.coli is an important pathogen leading to infant diarrhea,and highly resistant to many antibiotics; we should identify E.coli and select correct antibiotics according to the results of susceptibility test.
Objective:To survey the serotype and antibiotics resistance of enteropathogenic Escherichia coli(E.coli) in children with diarrhea in Nanjing area. Methods:Five thousand four hundred and nine cases of feces samples were cultured,isolated E.coli strains were indentified by serotyping,and the antibiotic susceptibility was determined by disk diffusion test. Results:Six hundred and thirtythree strains of E.coli were isolated(11.70%) and 12 serotypes were detected. The incidence of ESBLs was 82.15% in the isolated strains. Conclusions:E.coli is an important pathogen leading to infant diarrhea,and highly resistant to many antibiotics; we should identify E.coli and select correct antibiotics according to the results of susceptibility test.
2011, 36(12): 1382-1384.
Abstract:
Objective:To explore the value of serum cystatin C(Cys C) levels in determining the impairment of early renal function. Methods:Serum Cys C in 110 cases with renal function impairment was detected by an automated particle-enhanced turbidimetric immunoassay method,serum creatinine was determined by picricacid method and serum urea by urea enzymatic method,all of which were divided into 5 periods according to the level of glomerular filtration rate(eGFR),and the correlation analysis was carried out. Results:The serum level of Cys C increased with the decrease of the eGFR level in the 110 cases(P<0.01). The Cys C concentration levels had statistical meaning between the 5 periods(P<0.01). The abnormal detection in the 5 periods was 52.94%,64.29%, 92.00%,100.00% and 100.00%,respectively. The difference was significant(P<0.01). Conclusions:Serum Cys C is a sensitive indicator reflecting early renal damage,which is of great value in diagnosis of early renal diseases.
Objective:To explore the value of serum cystatin C(Cys C) levels in determining the impairment of early renal function. Methods:Serum Cys C in 110 cases with renal function impairment was detected by an automated particle-enhanced turbidimetric immunoassay method,serum creatinine was determined by picricacid method and serum urea by urea enzymatic method,all of which were divided into 5 periods according to the level of glomerular filtration rate(eGFR),and the correlation analysis was carried out. Results:The serum level of Cys C increased with the decrease of the eGFR level in the 110 cases(P<0.01). The Cys C concentration levels had statistical meaning between the 5 periods(P<0.01). The abnormal detection in the 5 periods was 52.94%,64.29%, 92.00%,100.00% and 100.00%,respectively. The difference was significant(P<0.01). Conclusions:Serum Cys C is a sensitive indicator reflecting early renal damage,which is of great value in diagnosis of early renal diseases.
2011, 36(12): 1385-1387.
Abstract:
Objective:To evaluate the results of serum electrolytes by two automatic biochemistry analyzer systems:7600-020 fullautomatic biochemical analyzer(Hitachi,Japan) and VITROS 350 dry-type biochemistry analyzer(Johnson,US),and assess the bias, which to ensure the consistency of test results. Methods:According to the National Committee for clinical laboratory standards EP9-A documents,7600-020 system was as control,Vitros-350 system was as experimental instrument,measure serum electrolytes by the two instruments,record the data,check the discrete point,calculate linear equations and correlation coefficients(r),and carry on the bias estimation. Results:Correlations between the two instruments were fine enough(P<0.05) and the clinical acceptability assessments did not exceed half of the acceptable error by CLIA'88. Conclusions:The electrolyte results which determined by Hitachi 7600-020 automatic biochemical analyzer and Johnson Vitros-350 dry type biochemistry analyzer are basically consistent and have a good comparability,either of the two instruments can be selected to do electrolyte determination.
Objective:To evaluate the results of serum electrolytes by two automatic biochemistry analyzer systems:7600-020 fullautomatic biochemical analyzer(Hitachi,Japan) and VITROS 350 dry-type biochemistry analyzer(Johnson,US),and assess the bias, which to ensure the consistency of test results. Methods:According to the National Committee for clinical laboratory standards EP9-A documents,7600-020 system was as control,Vitros-350 system was as experimental instrument,measure serum electrolytes by the two instruments,record the data,check the discrete point,calculate linear equations and correlation coefficients(r),and carry on the bias estimation. Results:Correlations between the two instruments were fine enough(P<0.05) and the clinical acceptability assessments did not exceed half of the acceptable error by CLIA'88. Conclusions:The electrolyte results which determined by Hitachi 7600-020 automatic biochemical analyzer and Johnson Vitros-350 dry type biochemistry analyzer are basically consistent and have a good comparability,either of the two instruments can be selected to do electrolyte determination.
2011, 36(12): 1388-1390.
Abstract:
Objective:To develop a duplex quantitative real-time PCR assay for rapid detection of Staphylococcus aureus and identification of methicilin resistant Staphylococcus aureus(MRSA). Methods:Two pairs of specific primers and TaqMan fluorescent probe were designed according to the conserved region of NUC gene sequences of Staphylococcus aureus and MecA gene sequences of MRSA,respectively. The 5' end labeled with different fluorescent reporter groups (FAM and HEX) and the 3' end labeled with fluorescence quenching group were used to detect Staphylococcus aureus and identify the gene of MRSA in order of evaluate its sensitivity and specificity. Results:The genes of NUC and MecA were specifically amplified in the duplex PCR reaction respectively. The MRSA in the study presented MecA and NUC gene,while methicillin susceptible Staphylococcus aureus strains had only a NUC gene; the same species of Staphylococcus epidermidis,Streptococcus faecalis and Micrococcus luteus had no specific amplification gene; at least 100MRSA strains were detected by using this duplex real-time PCR. Conclusions:The duplex real-time PCR assay can not only monitor the community and clinical-acquired MRSA infection, but provide necessary basis for pharmacotherapy to MRSA infection patients.
Objective:To develop a duplex quantitative real-time PCR assay for rapid detection of Staphylococcus aureus and identification of methicilin resistant Staphylococcus aureus(MRSA). Methods:Two pairs of specific primers and TaqMan fluorescent probe were designed according to the conserved region of NUC gene sequences of Staphylococcus aureus and MecA gene sequences of MRSA,respectively. The 5' end labeled with different fluorescent reporter groups (FAM and HEX) and the 3' end labeled with fluorescence quenching group were used to detect Staphylococcus aureus and identify the gene of MRSA in order of evaluate its sensitivity and specificity. Results:The genes of NUC and MecA were specifically amplified in the duplex PCR reaction respectively. The MRSA in the study presented MecA and NUC gene,while methicillin susceptible Staphylococcus aureus strains had only a NUC gene; the same species of Staphylococcus epidermidis,Streptococcus faecalis and Micrococcus luteus had no specific amplification gene; at least 100MRSA strains were detected by using this duplex real-time PCR. Conclusions:The duplex real-time PCR assay can not only monitor the community and clinical-acquired MRSA infection, but provide necessary basis for pharmacotherapy to MRSA infection patients.
2011, 36(12): 1391-1392.
Abstract:
Objective:To explore the cytomorphology and other related laboratory features of multiple myeloma(MM). Methods:The bone marrow cell morphology and related laboratory features of 32 patients with MM were retrospectively analyzed. Results:There were many laboratory test results of MM,such as anaemia,abnormal RBC distribution,higher sedimentation rate of red blood cell,remarkable increase of seroglobulin, decrease of albumin/globulin, albuminuria and so on. According to the bone marrow cell morphology, the multiple myeloma could be divided into 4 groups, as plasmocyte MM, proplasmacyte MM, plasmablast MM, and reticulocyte MM. Conclusions:Morphology observation of bone marrow cells is an important method in MM diagnosis,while blood routine examination, RBC distribution in blood smear,blood sedimentation and determination of serum protein also should be recommended. MM can be more exactly diagnosed by the examination of medullary cytology and the related laboratory examinations.
Objective:To explore the cytomorphology and other related laboratory features of multiple myeloma(MM). Methods:The bone marrow cell morphology and related laboratory features of 32 patients with MM were retrospectively analyzed. Results:There were many laboratory test results of MM,such as anaemia,abnormal RBC distribution,higher sedimentation rate of red blood cell,remarkable increase of seroglobulin, decrease of albumin/globulin, albuminuria and so on. According to the bone marrow cell morphology, the multiple myeloma could be divided into 4 groups, as plasmocyte MM, proplasmacyte MM, plasmablast MM, and reticulocyte MM. Conclusions:Morphology observation of bone marrow cells is an important method in MM diagnosis,while blood routine examination, RBC distribution in blood smear,blood sedimentation and determination of serum protein also should be recommended. MM can be more exactly diagnosed by the examination of medullary cytology and the related laboratory examinations.
2011, 36(12): 1402-1403.
Abstract:
Objective:To explore the safety suction method for adult acute respiratory distress syndrome (ARDS) patients who received mechanical ventilation. Methods:Forty ARDS patients who received mechanical ventilation by implementation of artifical airway were randomly divided into experimental and control groups,20 cases in each group. The patients in experimental group were performed with closed endotracheal suction by the tee tubes without offline and the patients in control group were performed common open style suction. The variations of partial pressure of oxygen in artery(PaO2),arterial oxygen saturation(SaO2),heart vatel(HR), diastolic blood pressure (DBP) and systolic blood pressure (SBP) were monitored in each group. Results:In control group after endotracheal suction,the levels of SaO2 and PaO2 were decreased, the levels of SBP and HR were increased (P<0.01), and no significant change of DBP(P>0.05). In experimental group after endotracheal suction,there had no significant changes of the levels of SaO2,PaO2 and DBP,HR and SBP were decreased (P>0.05). Compared with control group,the changes of PaO2 and SaO2 had significant differences in experimental group(P<0.01). Conclusions:Closed endotracheal suction by tee tubes without offline can improve blood oxygen levels in ARDS patients who received mechanical ventilation and maintain hemodynamical stablity.
Objective:To explore the safety suction method for adult acute respiratory distress syndrome (ARDS) patients who received mechanical ventilation. Methods:Forty ARDS patients who received mechanical ventilation by implementation of artifical airway were randomly divided into experimental and control groups,20 cases in each group. The patients in experimental group were performed with closed endotracheal suction by the tee tubes without offline and the patients in control group were performed common open style suction. The variations of partial pressure of oxygen in artery(PaO2),arterial oxygen saturation(SaO2),heart vatel(HR), diastolic blood pressure (DBP) and systolic blood pressure (SBP) were monitored in each group. Results:In control group after endotracheal suction,the levels of SaO2 and PaO2 were decreased, the levels of SBP and HR were increased (P<0.01), and no significant change of DBP(P>0.05). In experimental group after endotracheal suction,there had no significant changes of the levels of SaO2,PaO2 and DBP,HR and SBP were decreased (P>0.05). Compared with control group,the changes of PaO2 and SaO2 had significant differences in experimental group(P<0.01). Conclusions:Closed endotracheal suction by tee tubes without offline can improve blood oxygen levels in ARDS patients who received mechanical ventilation and maintain hemodynamical stablity.
2011, 36(12): 1404-1406.
Abstract:
Objective:To evaluate the nursing methods on lung cancer patients treated with CT-guided percutaneous radiofrequency ablation(RFA) under intravenous anesthesia. Methods:Eleven patients were treated with CT-guided RFA. All patients were received health education and psychological nursing before operation,careful co-operating during operation and corresponding observation after operation. Results:The successful rate of RFA was 100%. The complications were not observed during operation,however,after RFA, the symptom of fever from 37.5 to 38.6℃ was appeared in 5 cases,chest pain happened in 3 cases and hemoptysis in 3 cases. These complications were quickly disappeared through corresponding treatment and enhancing nursing. Conclusions:Intensive nursing and active care during perioperative period of RFA are contributed to increase the successful rate of operation and decrease the incidence of complication. It is necessary to discover and deal with the complications as early as possible and promote postoperative recovery.
Objective:To evaluate the nursing methods on lung cancer patients treated with CT-guided percutaneous radiofrequency ablation(RFA) under intravenous anesthesia. Methods:Eleven patients were treated with CT-guided RFA. All patients were received health education and psychological nursing before operation,careful co-operating during operation and corresponding observation after operation. Results:The successful rate of RFA was 100%. The complications were not observed during operation,however,after RFA, the symptom of fever from 37.5 to 38.6℃ was appeared in 5 cases,chest pain happened in 3 cases and hemoptysis in 3 cases. These complications were quickly disappeared through corresponding treatment and enhancing nursing. Conclusions:Intensive nursing and active care during perioperative period of RFA are contributed to increase the successful rate of operation and decrease the incidence of complication. It is necessary to discover and deal with the complications as early as possible and promote postoperative recovery.
2011, 36(12): 1407-1409.
Abstract:
Objective:To explore the efficacy of barrier technology in management of portable blood glucose meters and provide accurate reference indexes for clinical application. Methods:The barrier technology was adopted in management of portable blood glucose meters,and the data collected were statistically analyzed. Results:The barrier technology improved the nursing staff'operative skills in using blood glucose meters,and the correct rate increased. Conclusions:The barrier technology can improve the management of the portable blood glucose meter.
Objective:To explore the efficacy of barrier technology in management of portable blood glucose meters and provide accurate reference indexes for clinical application. Methods:The barrier technology was adopted in management of portable blood glucose meters,and the data collected were statistically analyzed. Results:The barrier technology improved the nursing staff'operative skills in using blood glucose meters,and the correct rate increased. Conclusions:The barrier technology can improve the management of the portable blood glucose meter.
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