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2011 Vol. 36, No. 2
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2011, 36(2): 109-111,116.
Abstract:
Objective: To investigate the mechanism of remote ischemic postconditioning (RPostC) induced cardioprotection against ischemia/reperfusion (I/R) injury in rat model. Methods: Anesthetized male Sprague Dawley rats were randomly divided into the two groups,ischemia/reperfusion (I/R,n=12) and remote ischemic postconditioning group (RPostC,n=12). Throughout the experiment,a lead Ⅱ electrocardiogram was continuously monitored. At the end of reperfusion,arterial blood sample was obtained to quantify plasma activity of lactate dehydrogenase (LDH) and creatine kinase (CK). The infarct size was measured by triphenyltetrazolium chloride staining method. The expressions of Bcl-2 and Bax at mRNA level of left anterior myocardium were detected by RT-PCR analysis. Results: Compared with the I/R group,the levels of ventricular arrhythmia,CK,LDH release and infarct size in RPostC group were reduced significantly (P < 0.01 and P < 0.05); and the ratio of Bcl-2/Bax mRNA expression was increased (P < 0.05). Conclusions: Remote ischemic postconditioning has the significant protective effect on rat against myocardial ischemia and reperfusion injury,and the anti-apoptosis might be a mechanisms of cardioprotection.
Objective: To investigate the mechanism of remote ischemic postconditioning (RPostC) induced cardioprotection against ischemia/reperfusion (I/R) injury in rat model. Methods: Anesthetized male Sprague Dawley rats were randomly divided into the two groups,ischemia/reperfusion (I/R,n=12) and remote ischemic postconditioning group (RPostC,n=12). Throughout the experiment,a lead Ⅱ electrocardiogram was continuously monitored. At the end of reperfusion,arterial blood sample was obtained to quantify plasma activity of lactate dehydrogenase (LDH) and creatine kinase (CK). The infarct size was measured by triphenyltetrazolium chloride staining method. The expressions of Bcl-2 and Bax at mRNA level of left anterior myocardium were detected by RT-PCR analysis. Results: Compared with the I/R group,the levels of ventricular arrhythmia,CK,LDH release and infarct size in RPostC group were reduced significantly (P < 0.01 and P < 0.05); and the ratio of Bcl-2/Bax mRNA expression was increased (P < 0.05). Conclusions: Remote ischemic postconditioning has the significant protective effect on rat against myocardial ischemia and reperfusion injury,and the anti-apoptosis might be a mechanisms of cardioprotection.
2011, 36(2): 112-116.
Abstract:
Objective: To study the expression of monocyte chemoattractant protein-1 (MCP-1) and mRNA in the renal tubules of STZ-induced diabetic nephropathy rats. Methods: Sixty SD rats were randomly divided into two groups:normal control (n=10) and diabetic nephropathy group (n=50). Diabetes was induced by intraperitoneal injection of STZ. And the diabetic rats were randomly divided into model group, irbesartan treatment group, haikunshenxi at low and high doses treatment group. Twenty four-hour urine volume,24-hour urinary protein excretion were examined at weeks 4,8,and 12. All rats were sacrificed at week 12; body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge were improved by haikunshenxi preparation. The expression of MCP-1 and mRNA in rat kidney were detected respectively by immunohistochemistry and in situ hybridization. Results: The expression of MCP-1and mRNA in haikunshenxi treatment group was depressed greatly in the renal tubular epithelial cell and renal interstitial cell (P < 0.01). Conclusions: Haikunshenxi can inhibit the overexpression of MCP-1 and mRNA in kidney of diabetic rats and delay the progress of diabetic nephropathy.
Objective: To study the expression of monocyte chemoattractant protein-1 (MCP-1) and mRNA in the renal tubules of STZ-induced diabetic nephropathy rats. Methods: Sixty SD rats were randomly divided into two groups:normal control (n=10) and diabetic nephropathy group (n=50). Diabetes was induced by intraperitoneal injection of STZ. And the diabetic rats were randomly divided into model group, irbesartan treatment group, haikunshenxi at low and high doses treatment group. Twenty four-hour urine volume,24-hour urinary protein excretion were examined at weeks 4,8,and 12. All rats were sacrificed at week 12; body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge were improved by haikunshenxi preparation. The expression of MCP-1 and mRNA in rat kidney were detected respectively by immunohistochemistry and in situ hybridization. Results: The expression of MCP-1and mRNA in haikunshenxi treatment group was depressed greatly in the renal tubular epithelial cell and renal interstitial cell (P < 0.01). Conclusions: Haikunshenxi can inhibit the overexpression of MCP-1 and mRNA in kidney of diabetic rats and delay the progress of diabetic nephropathy.
2011, 36(2): 117-120.
Abstract:
Objective: To observe the effect of homocysteine (Hcy) on apoptosis and activation of nuclear factor-κB (NF-κB) in ECV-304 cells and investigate the possible mechanism in Hcy induced atherosclerosis. Methods: The apoptosis of ECV-304 cells was detected by PI staining. The expression of Bcl-2 and the location of NF-κB in cells were examined by immunohistochemistry method. The expression of NF-κB in cell nuclear was detected by flow cytometry. Results: Hcy induced apoptosis of ECV-304 cells and the apoptosis index were increased with Hcy concentration and treatment time. When the cells were treated with 10.0 mmol/L Hcy for 24 hours,the number of positive cells in expression of Bcl-2 was increased significantly compared with 1.0,5.0 mmol/L and control group (P < 0.01). In control group,NF-κB was located in cytoplasma,when the cells were incubated with 5 and 10 mmol/L Hcy,NF-κB was transferred into cellular nuclear. After incubation with 5 mmol/L Hcy for 0.5,1,and 2 hours,the expression rate of NF-κB in cell nuclear was (16.76 ±2.55)%, (12.91 ±1.38)% and (14.15 ±1.74)%,respectively. Conclusions: Hcy can induce apoptosis of ECV-304 cells through reducing the expression Bcl-2 in dose-dependent manner. Hcy can activate NF-κB in a concentration-dependent pattern.
Objective: To observe the effect of homocysteine (Hcy) on apoptosis and activation of nuclear factor-κB (NF-κB) in ECV-304 cells and investigate the possible mechanism in Hcy induced atherosclerosis. Methods: The apoptosis of ECV-304 cells was detected by PI staining. The expression of Bcl-2 and the location of NF-κB in cells were examined by immunohistochemistry method. The expression of NF-κB in cell nuclear was detected by flow cytometry. Results: Hcy induced apoptosis of ECV-304 cells and the apoptosis index were increased with Hcy concentration and treatment time. When the cells were treated with 10.0 mmol/L Hcy for 24 hours,the number of positive cells in expression of Bcl-2 was increased significantly compared with 1.0,5.0 mmol/L and control group (P < 0.01). In control group,NF-κB was located in cytoplasma,when the cells were incubated with 5 and 10 mmol/L Hcy,NF-κB was transferred into cellular nuclear. After incubation with 5 mmol/L Hcy for 0.5,1,and 2 hours,the expression rate of NF-κB in cell nuclear was (16.76 ±2.55)%, (12.91 ±1.38)% and (14.15 ±1.74)%,respectively. Conclusions: Hcy can induce apoptosis of ECV-304 cells through reducing the expression Bcl-2 in dose-dependent manner. Hcy can activate NF-κB in a concentration-dependent pattern.
2011, 36(2): 121-123.
Abstract:
Objective: To establish a suitable primary culture method of rat cerebellar granule neurons. Methods: Rat cerebellar granule neurons were prepared from 5-7 day old Sprague-Dawley rat pups,the cerebella was freed of meninges,minced,trypsinized, then the cell suspension was preplated for 30 min for remove any glial cells,dissociated cells were seeded at plates which had been pre-coated with Poly-L-Lysine,arabinosylcytosine was added to the culture medium on day 3 after seeding for inhibition of non-neuronal cell division. Neurons were identified by neuron-specific enolase immunofluorescence technic. Results: The survival rate of the cells was (98 ±1.07)%; the neurons were affixed to the culture plate after 24 hours,neurite growth was apparently on day 3,integrated neural network was formed on day 6-8. Cerebellar granule neurons was about 90% by neuron-specific enolase identifying. Conclusions: Neuron purity was higher in the experiment; it is a perfect technique for primary culture of rat cerebellar granule neurons.
Objective: To establish a suitable primary culture method of rat cerebellar granule neurons. Methods: Rat cerebellar granule neurons were prepared from 5-7 day old Sprague-Dawley rat pups,the cerebella was freed of meninges,minced,trypsinized, then the cell suspension was preplated for 30 min for remove any glial cells,dissociated cells were seeded at plates which had been pre-coated with Poly-L-Lysine,arabinosylcytosine was added to the culture medium on day 3 after seeding for inhibition of non-neuronal cell division. Neurons were identified by neuron-specific enolase immunofluorescence technic. Results: The survival rate of the cells was (98 ±1.07)%; the neurons were affixed to the culture plate after 24 hours,neurite growth was apparently on day 3,integrated neural network was formed on day 6-8. Cerebellar granule neurons was about 90% by neuron-specific enolase identifying. Conclusions: Neuron purity was higher in the experiment; it is a perfect technique for primary culture of rat cerebellar granule neurons.
2011, 36(2): 124-126,129.
Abstract:
Objective: To observe the changes of plasma high mobility group protein -1 (HMGB-1) in rabbits after acute CO poisoning dynamically,discuss the association between HMGB-1 and delayed neuropsychological sequelae,and to analyze its possible mechanism in the course of the disease. Methods: Twenty-four healthy rabbits were randomly divided into control group,delayed neuropsychological sequelae after acute CO poisoning group (model group),and sodium butyrate pretreatment group (intervention group),8 in each. The rabbits were injected continuously interval high purity CO gas intraperitoneally to prepare the model of delayed neuropsychological sequelae after acute CO poisoning. After the end of modeling,1,3,6,12,24 h,and 3,7,14,21 d,blood was drawn from ear vein each time to test the levels of HMGB-1. Results: The plasma HMGB-1 levels at 3 h after the end of modeling in model group had statistical difference in contrast to control group (P < 0.05). From 6 h to 21 d after the end of modeling,the differences between model group and control group were all significance (P < 0.01),while were not statistical difference between intervention group and control group (P > 0.05); from 6 h to 21 d,in poisoned rabbits (model group and intervention group),the rate of delayed encephalopathy was significant difference compared to no encephalopathy happening (P < 0.01). Conclusions: Early plasma HMGB-1 levels are highly correlated with delayed neuropsychological sequelae after acute CO poisoning, and HMGB-1 can play a crucial role in the formation of delayed encephalopathy.
Objective: To observe the changes of plasma high mobility group protein -1 (HMGB-1) in rabbits after acute CO poisoning dynamically,discuss the association between HMGB-1 and delayed neuropsychological sequelae,and to analyze its possible mechanism in the course of the disease. Methods: Twenty-four healthy rabbits were randomly divided into control group,delayed neuropsychological sequelae after acute CO poisoning group (model group),and sodium butyrate pretreatment group (intervention group),8 in each. The rabbits were injected continuously interval high purity CO gas intraperitoneally to prepare the model of delayed neuropsychological sequelae after acute CO poisoning. After the end of modeling,1,3,6,12,24 h,and 3,7,14,21 d,blood was drawn from ear vein each time to test the levels of HMGB-1. Results: The plasma HMGB-1 levels at 3 h after the end of modeling in model group had statistical difference in contrast to control group (P < 0.05). From 6 h to 21 d after the end of modeling,the differences between model group and control group were all significance (P < 0.01),while were not statistical difference between intervention group and control group (P > 0.05); from 6 h to 21 d,in poisoned rabbits (model group and intervention group),the rate of delayed encephalopathy was significant difference compared to no encephalopathy happening (P < 0.01). Conclusions: Early plasma HMGB-1 levels are highly correlated with delayed neuropsychological sequelae after acute CO poisoning, and HMGB-1 can play a crucial role in the formation of delayed encephalopathy.
2011, 36(2): 127-129.
Abstract:
Objective: To observe the changes of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6),and their influence on the blood pressure and heart rates of rats by establishing models of chronic stress. Methods: Forty healthy male SD rats were randomly divided into two groups. Control group (n=10) were not given any incentives,and the experimental group (n=30) were established models of chronic stress with Katz method. Serum TNF-α and IL-6 levels of the rats were measured by enzyme-linked immunoassay,and the blood pressure and heart rates were recorded. Results: Compared with the control group,the open field activity of the rats with chronic stress decreased (P < 0.01),serum IL-6 and TNF-α levels increased (P < 0.05),the blood pressure was elevated and the heart rate speeded (P < 0.01). Conclusions: Stress may affect the emotional behavior,increase the serum IL-6 and TNF-α levels and influence the blood pressure and heart rates of the rats.
Objective: To observe the changes of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6),and their influence on the blood pressure and heart rates of rats by establishing models of chronic stress. Methods: Forty healthy male SD rats were randomly divided into two groups. Control group (n=10) were not given any incentives,and the experimental group (n=30) were established models of chronic stress with Katz method. Serum TNF-α and IL-6 levels of the rats were measured by enzyme-linked immunoassay,and the blood pressure and heart rates were recorded. Results: Compared with the control group,the open field activity of the rats with chronic stress decreased (P < 0.01),serum IL-6 and TNF-α levels increased (P < 0.05),the blood pressure was elevated and the heart rate speeded (P < 0.01). Conclusions: Stress may affect the emotional behavior,increase the serum IL-6 and TNF-α levels and influence the blood pressure and heart rates of the rats.
2011, 36(2): 130-132.
Abstract:
Objective: To observe the features of pathological structure of pneum-arteriolar in complex congenital heart disease with diminutive pulmonary blood. Methods: Twenty-eight patients suffering from congenital heart disease with diminutive pulmonary blood (study group) and 28 matched volunteers (sex,age and body weight) without cardiopulmonary disease (control group) were select to obtain right middle lobe lung tissue pieces (1 cm×1 cm×1 cm) after thoracotomy. Optical microscope was connected biomedical image analysis,the pulmonary arterioles with the diameter ≤ 100 μm and cross-section complete were select to measure the pulmonary arterioles outside and inside diameter of five pulmonary arterioles in lung tissue, and the average percentage of media thickness (mMTPA) was calculate. After taking the average the total vascular area of pulmonary arterioles,vascular lumen area,vessel wall area, vessel wall area/vessel total area (WA/TA),vascular lumen area/vessel total area (EA/TA),and perunit area the number of pulmonary arterioles (APSC) were calculate. Results: The outside diameter of pulmonary arterioles in study group was no significant difference compared with the control group (P > 0.05),and the inside diameter of pulmonary arterioles in study group was higher (P < 0.01),the mMTPA,WA/TA and APSC of pulmonary arterioles in study group was significantly lower (P < 0.01). Conclusions: Tunica media of pulmonary artery was thiner and vascular lumen expanded, APSC of per unit area was reducing in congenital heart disease with diminutive pulmonary blood.
Objective: To observe the features of pathological structure of pneum-arteriolar in complex congenital heart disease with diminutive pulmonary blood. Methods: Twenty-eight patients suffering from congenital heart disease with diminutive pulmonary blood (study group) and 28 matched volunteers (sex,age and body weight) without cardiopulmonary disease (control group) were select to obtain right middle lobe lung tissue pieces (1 cm×1 cm×1 cm) after thoracotomy. Optical microscope was connected biomedical image analysis,the pulmonary arterioles with the diameter ≤ 100 μm and cross-section complete were select to measure the pulmonary arterioles outside and inside diameter of five pulmonary arterioles in lung tissue, and the average percentage of media thickness (mMTPA) was calculate. After taking the average the total vascular area of pulmonary arterioles,vascular lumen area,vessel wall area, vessel wall area/vessel total area (WA/TA),vascular lumen area/vessel total area (EA/TA),and perunit area the number of pulmonary arterioles (APSC) were calculate. Results: The outside diameter of pulmonary arterioles in study group was no significant difference compared with the control group (P > 0.05),and the inside diameter of pulmonary arterioles in study group was higher (P < 0.01),the mMTPA,WA/TA and APSC of pulmonary arterioles in study group was significantly lower (P < 0.01). Conclusions: Tunica media of pulmonary artery was thiner and vascular lumen expanded, APSC of per unit area was reducing in congenital heart disease with diminutive pulmonary blood.
2011, 36(2): 133-136.
Abstract:
Objective: To observe the expression of epithelial cell adhesion molecule (Ep-CAM) and heparanase (Hpa) in primary gallbladder carcinoma,and to investigate their role in the genesis and development of primary gallbladder carcinoma. Methods: Ep-CAM protein and Hpa protein were determined in 50 cases of primary gallbladder carcinoma,20 cases of gallbladder adenoma and 20 cases of chronic cholecystitis using immunohistochemistry EliVisionTM plus staining method. Results: The positive rate of Ep-CAM expression in gallbladder carcinoma was higher than that in cholecystitis (P < 0.05). The positive rate of Hpa expression in gallbladder carcinoma was higher than that in gallbladder adenoma and cholecystitis (P < 0.05). The positive rate of Ep-CAM expression and Hpa in gallbladder carcinoma was related with the Nevin stages (P < 0.05 and P < 0.01). The median survival time of the tumor patients with or without overexpression of Ep-CAM was 13 and 24 months, respectively. Conclusions: The expression of Ep-CAM and Hpa may play an important part in the genesis and progression of gallbladder carcinoma,and may act as a marker for gallbladder carcinoma metastasis. Ep-CAM can be used as a prognostic marker for patients with advanced gallbladder carcinoma.
Objective: To observe the expression of epithelial cell adhesion molecule (Ep-CAM) and heparanase (Hpa) in primary gallbladder carcinoma,and to investigate their role in the genesis and development of primary gallbladder carcinoma. Methods: Ep-CAM protein and Hpa protein were determined in 50 cases of primary gallbladder carcinoma,20 cases of gallbladder adenoma and 20 cases of chronic cholecystitis using immunohistochemistry EliVisionTM plus staining method. Results: The positive rate of Ep-CAM expression in gallbladder carcinoma was higher than that in cholecystitis (P < 0.05). The positive rate of Hpa expression in gallbladder carcinoma was higher than that in gallbladder adenoma and cholecystitis (P < 0.05). The positive rate of Ep-CAM expression and Hpa in gallbladder carcinoma was related with the Nevin stages (P < 0.05 and P < 0.01). The median survival time of the tumor patients with or without overexpression of Ep-CAM was 13 and 24 months, respectively. Conclusions: The expression of Ep-CAM and Hpa may play an important part in the genesis and progression of gallbladder carcinoma,and may act as a marker for gallbladder carcinoma metastasis. Ep-CAM can be used as a prognostic marker for patients with advanced gallbladder carcinoma.
2011, 36(2): 137-138,141.
Abstract:
Objective: To observe the therapeutic effect of endovenous laser treatment (EVLT) combined with high ligation and punctate stripping on simple great saphenous varicosities. Methods: The data of 8 patients (11 lower limbs) who had received EVLT plus high ligation and punctate stripping were retrospectively analyzed. Results: The outcome was satisfactory in all the cases,and the recent symptoms disappeared. No relapse of varicose vein or lower limb swelling was observed during the follow-up of 1-7 months. Two cases (2 lower limbs) had thermal burns of anterior tibial skin and 1 case (1 lower limb) subcutaneous ecchymosis. Conclusions: EVLT combined with high ligation and punctate stripping has affirmed effect in treatment of great saphenous varicosity with the advantage of few complications. It is a safe and reliable minimally invasive therapy.
Objective: To observe the therapeutic effect of endovenous laser treatment (EVLT) combined with high ligation and punctate stripping on simple great saphenous varicosities. Methods: The data of 8 patients (11 lower limbs) who had received EVLT plus high ligation and punctate stripping were retrospectively analyzed. Results: The outcome was satisfactory in all the cases,and the recent symptoms disappeared. No relapse of varicose vein or lower limb swelling was observed during the follow-up of 1-7 months. Two cases (2 lower limbs) had thermal burns of anterior tibial skin and 1 case (1 lower limb) subcutaneous ecchymosis. Conclusions: EVLT combined with high ligation and punctate stripping has affirmed effect in treatment of great saphenous varicosity with the advantage of few complications. It is a safe and reliable minimally invasive therapy.
2011, 36(2): 139-141.
Abstract:
Objective: To investigate the clinical pathologic characteristics,histogenesis,diagnosis and differential diagnosis of primary diffuse large B-cell lymphoma (DLBCL) of the breast. Methods: Two cases of breast DLBCL were analyzed by immunohistochemistry and morphology,and the related literatures were reviewed. Results: Two patients both noticed a mass in their left breast,and their ages were 46 and 42 years. The tumors were well circumscribed accompanied by the focal region proliferation response of lymphocyte. The tumor cells widespreadly infiltrated in the surrounding tissue. The nucleus of tumor cells were round, irregular and unusual large, accompanied by apoptosis and focal necrosis. Conclusions: Primary DLBCL of the breast,which the tumorgenesis and morphology are similar to lymphoma in other parts,is a rare subtype lymphoma of non-lymph node origin. The recent development of immunohistochemical markers may be useful to distinguish the breast cancer and sarcoma in addition to conventional histological criteria for diagnosis.
Objective: To investigate the clinical pathologic characteristics,histogenesis,diagnosis and differential diagnosis of primary diffuse large B-cell lymphoma (DLBCL) of the breast. Methods: Two cases of breast DLBCL were analyzed by immunohistochemistry and morphology,and the related literatures were reviewed. Results: Two patients both noticed a mass in their left breast,and their ages were 46 and 42 years. The tumors were well circumscribed accompanied by the focal region proliferation response of lymphocyte. The tumor cells widespreadly infiltrated in the surrounding tissue. The nucleus of tumor cells were round, irregular and unusual large, accompanied by apoptosis and focal necrosis. Conclusions: Primary DLBCL of the breast,which the tumorgenesis and morphology are similar to lymphoma in other parts,is a rare subtype lymphoma of non-lymph node origin. The recent development of immunohistochemical markers may be useful to distinguish the breast cancer and sarcoma in addition to conventional histological criteria for diagnosis.
2011, 36(2): 142-144.
Abstract:
Objective: To investigate the influence of flurbiprofen on cytokines tumor necrosis factor-α (TNF-α),and interleukin-6 (IL-6) in children undergoing cardiopulmonary bypass (CPB). Methods: In this pilot, prospective, and randomized double-blinded study,twenty children with cardiac function classⅠ-Ⅱ scheduled for repairing ventricular septal defect were divided into two groups:children in flurbiprofen group (F group,n=10) were received 1 mg/kg flurbiprofen 15 min before skin incision and children in control group (C group,n=10) were received 0.1 ml/kg intralipid instead of flurbiprofen. Blood samples were taken 10 min before skin incision (T1),10 min after aorta declamped (T2), 30 min (T3), 4 h (T4), 24 h (T5), 48 h (T6) after termination of CPB for determination of plasma TNF-α and IL-6 concentration. Results: The plasma TNF-α levels was higher at T3 and T5 compared to baseline (P < 0.05-P < 0.01),but F group was lower than in C group at T3 (P < 0.01). IL-6 was higher during T2-T6 in C group (P < 0.05-P < 0.01) compared to T1,but F group was lower than C group at T2,T3 and T6 (P < 0.01). Conclusions: Flurbiprofen is effective for reducing CPB-induced inflammatory response and it is benefitful for maintaining stability of circulation.
Objective: To investigate the influence of flurbiprofen on cytokines tumor necrosis factor-α (TNF-α),and interleukin-6 (IL-6) in children undergoing cardiopulmonary bypass (CPB). Methods: In this pilot, prospective, and randomized double-blinded study,twenty children with cardiac function classⅠ-Ⅱ scheduled for repairing ventricular septal defect were divided into two groups:children in flurbiprofen group (F group,n=10) were received 1 mg/kg flurbiprofen 15 min before skin incision and children in control group (C group,n=10) were received 0.1 ml/kg intralipid instead of flurbiprofen. Blood samples were taken 10 min before skin incision (T1),10 min after aorta declamped (T2), 30 min (T3), 4 h (T4), 24 h (T5), 48 h (T6) after termination of CPB for determination of plasma TNF-α and IL-6 concentration. Results: The plasma TNF-α levels was higher at T3 and T5 compared to baseline (P < 0.05-P < 0.01),but F group was lower than in C group at T3 (P < 0.01). IL-6 was higher during T2-T6 in C group (P < 0.05-P < 0.01) compared to T1,but F group was lower than C group at T2,T3 and T6 (P < 0.01). Conclusions: Flurbiprofen is effective for reducing CPB-induced inflammatory response and it is benefitful for maintaining stability of circulation.
2011, 36(2): 145-146,149.
Abstract:
Objective: To explore the early diagnosis and treatment of testicular torsion so as to improve the treatment efficacy. Methods: Color Doppler showed that the blood flow of the testis decreased obviously in 2 cases and disappeared in 11 cases. Thir teen cases of testicular torsion were treated by surgery. Results: All the 13 cases were proved to be testicular torsion by surgical exploration. Ten cases of testicular torsion occurred on the left side and 3 cases on the right. Necrosis was observed in 11 cases and the testis was removed; the testis of the other 2 cases changed red after reset and was reserved. Conclusions: Early diagnosis of testicular torsion is very important. External application of ice cap may be one of the ways to prevent necrosis of the reversed testis. Early surgical exploration is essential to retain the reversed testis.
Objective: To explore the early diagnosis and treatment of testicular torsion so as to improve the treatment efficacy. Methods: Color Doppler showed that the blood flow of the testis decreased obviously in 2 cases and disappeared in 11 cases. Thir teen cases of testicular torsion were treated by surgery. Results: All the 13 cases were proved to be testicular torsion by surgical exploration. Ten cases of testicular torsion occurred on the left side and 3 cases on the right. Necrosis was observed in 11 cases and the testis was removed; the testis of the other 2 cases changed red after reset and was reserved. Conclusions: Early diagnosis of testicular torsion is very important. External application of ice cap may be one of the ways to prevent necrosis of the reversed testis. Early surgical exploration is essential to retain the reversed testis.
2011, 36(2): 147-149.
Abstract:
Objective: To observe the effect of free anterolateral thigh flap on soft tissue defect repair. Methods: Twenty-five cases with defects in different parts of limb were repaired by anterolateral thigh flap transplantation. In all of these cases,there were 14 cases repaired simply with free anterolateral thigh flap,while 1 case associated with free fibula transplantation and 3 cases associated with thumb reconstruction simultaneously. Anterolateral femoral nerves with end branches were connected with nerves of accepted zone by anastomosis in 14 cases. Results: Twenty-three pieces of skin flap survived completely; vascular crisis and skin necrosis occurred in 2 cases; 1 case of palm and 2 cases foot back flap transplantation were treated by additional thinner surgery for their less satisfied shapes. Follow-up by 6 to 24 months,14 cases of flap transplantation with nerve anastomosis acquired different degrees of sensory nerve recovery,as five cases of S2,four cases of S3 and three cases of S4. Twenty-three cases satisfied with the recovery of organ function and the appearance of the surgical sites. Conclusions: The free anterolateral thigh flap has many advantages,such as high survival rate and beautiful shape,so it is ideal for repairing tissue defects of limb flap and especially fit for young patients with cosmetic requirements.
Objective: To observe the effect of free anterolateral thigh flap on soft tissue defect repair. Methods: Twenty-five cases with defects in different parts of limb were repaired by anterolateral thigh flap transplantation. In all of these cases,there were 14 cases repaired simply with free anterolateral thigh flap,while 1 case associated with free fibula transplantation and 3 cases associated with thumb reconstruction simultaneously. Anterolateral femoral nerves with end branches were connected with nerves of accepted zone by anastomosis in 14 cases. Results: Twenty-three pieces of skin flap survived completely; vascular crisis and skin necrosis occurred in 2 cases; 1 case of palm and 2 cases foot back flap transplantation were treated by additional thinner surgery for their less satisfied shapes. Follow-up by 6 to 24 months,14 cases of flap transplantation with nerve anastomosis acquired different degrees of sensory nerve recovery,as five cases of S2,four cases of S3 and three cases of S4. Twenty-three cases satisfied with the recovery of organ function and the appearance of the surgical sites. Conclusions: The free anterolateral thigh flap has many advantages,such as high survival rate and beautiful shape,so it is ideal for repairing tissue defects of limb flap and especially fit for young patients with cosmetic requirements.
2011, 36(2): 150-152,155.
Abstract:
Objective: To investigate the clinicopathological features,diagnosis and differential diagnosis for metastatic neuroendocrine carcinoma of the spleen. Methods: Paraffin imbedding section,morphological observation and immunohistochemistry,with analysis of clinical manifestation and imaging data,were applied in one case of metastatic neuroendocrine carcinoma of the spleen,and the related literatures were reviewed. Results: A 30-year-old male presented lassitude, fever, pain in the left upper quadrant, moderate splenomegaly,splenic rupture. Colored Doppler and computed tomography showed space-occupying lesions in the spleen. Pathologic features:tumor cells were located in splenic pulp around splenic spontaneous rupture site and mass in hilum of spleen. Tumor cells were arranged in sheets or in trabs or in glandular cavity or in pseudo-rosettes,and were separated with fibrous stroma. The tumor cells were moderate size and relatively uniform,with abundant eosinophilic cytoplasm and round or orbicular-ovate nuclei with prominent nucleoli. Few pathological karyokinesis was observed. Lymph nodes in hilum of spleen and blood vessel were also invaded. The tumor cells were strongly positive for CD56,poorly positive for Syn,CgA and CK. Conclusions: Metastatic neuroendocrine carcinoma of the spleen is extremely rare. The morphology is quite similar to the neuroendocrine carcinoma in other organs,and immunohistochemistry is helpful in the diagnosis. Cooperation of clinicians and pathologists may enhance the diagnostic level.
Objective: To investigate the clinicopathological features,diagnosis and differential diagnosis for metastatic neuroendocrine carcinoma of the spleen. Methods: Paraffin imbedding section,morphological observation and immunohistochemistry,with analysis of clinical manifestation and imaging data,were applied in one case of metastatic neuroendocrine carcinoma of the spleen,and the related literatures were reviewed. Results: A 30-year-old male presented lassitude, fever, pain in the left upper quadrant, moderate splenomegaly,splenic rupture. Colored Doppler and computed tomography showed space-occupying lesions in the spleen. Pathologic features:tumor cells were located in splenic pulp around splenic spontaneous rupture site and mass in hilum of spleen. Tumor cells were arranged in sheets or in trabs or in glandular cavity or in pseudo-rosettes,and were separated with fibrous stroma. The tumor cells were moderate size and relatively uniform,with abundant eosinophilic cytoplasm and round or orbicular-ovate nuclei with prominent nucleoli. Few pathological karyokinesis was observed. Lymph nodes in hilum of spleen and blood vessel were also invaded. The tumor cells were strongly positive for CD56,poorly positive for Syn,CgA and CK. Conclusions: Metastatic neuroendocrine carcinoma of the spleen is extremely rare. The morphology is quite similar to the neuroendocrine carcinoma in other organs,and immunohistochemistry is helpful in the diagnosis. Cooperation of clinicians and pathologists may enhance the diagnostic level.
2011, 36(2): 153-155.
Abstract:
Objective: To analyze the clinical effect of three different kinds of internal fixations in treatment of the femoral intertrochanteric fracture. Methods: The clinical data of 80 patients with the femoral intertrochanteric fracture having been treated with the procedure of locking compression plate (LCP), dynamic hip screw (DHS), or proximal femoral nail (PFN) were analyzed retrospectively. The mean operation time,cut length,blood loss,healing time,incidence of the complication and rate of excellent and good results of the three groups were compared and analyzed. Results: There were statistical differences in the mean operation time,cut length,blood loss and healing time among the three groups (P < 0.01). The incidence of complications showed no statistical difference among the three groups (P > 0.05). As to the rate of excellent and good results, LCP group were superior to DHS group, but the difference was not significant among the three groups (P > 0.05). Conclusions: All the three procedures are effective. However,LCP fixation takes less operation time,less healing time,causes less surgical trauma and demonstrates better recovery than the other two groups in treatment of the intertrochanteric fracture.
Objective: To analyze the clinical effect of three different kinds of internal fixations in treatment of the femoral intertrochanteric fracture. Methods: The clinical data of 80 patients with the femoral intertrochanteric fracture having been treated with the procedure of locking compression plate (LCP), dynamic hip screw (DHS), or proximal femoral nail (PFN) were analyzed retrospectively. The mean operation time,cut length,blood loss,healing time,incidence of the complication and rate of excellent and good results of the three groups were compared and analyzed. Results: There were statistical differences in the mean operation time,cut length,blood loss and healing time among the three groups (P < 0.01). The incidence of complications showed no statistical difference among the three groups (P > 0.05). As to the rate of excellent and good results, LCP group were superior to DHS group, but the difference was not significant among the three groups (P > 0.05). Conclusions: All the three procedures are effective. However,LCP fixation takes less operation time,less healing time,causes less surgical trauma and demonstrates better recovery than the other two groups in treatment of the intertrochanteric fracture.
2011, 36(2): 156-157,160.
Abstract:
Objective: To observe the changes and the clinical significances of blood lipids in patients with rheumatoid arthritis (RA) in active phase. Methods: Fifty-six patients with RA (30 cases of active type,26 non-active) and 30 healthy controls were randomly chosen. Plasma lipids,erythrocyte sedimentation rate (ESR),C reactive protein (CRP) and rheumatoid factor (RF) were studied. All data were subjected to statistical analysis. Results: The high-density lipoprotein cholesterol (HDL-C) level in active RA group was significantly decreased as compared with normal control group (P < 0.01); there was no difference in HDL-C level between non-active RA group and normal control group (P > 0.05); however, levels of serum total cholesterol, triglyceride and low-density lipoprotein cholesterol had no significant differences among these three groups (P > 0.05). Conclusions: The levels of plasma lipids in patient with RA are abnormal,which is related to inflammation activity.
Objective: To observe the changes and the clinical significances of blood lipids in patients with rheumatoid arthritis (RA) in active phase. Methods: Fifty-six patients with RA (30 cases of active type,26 non-active) and 30 healthy controls were randomly chosen. Plasma lipids,erythrocyte sedimentation rate (ESR),C reactive protein (CRP) and rheumatoid factor (RF) were studied. All data were subjected to statistical analysis. Results: The high-density lipoprotein cholesterol (HDL-C) level in active RA group was significantly decreased as compared with normal control group (P < 0.01); there was no difference in HDL-C level between non-active RA group and normal control group (P > 0.05); however, levels of serum total cholesterol, triglyceride and low-density lipoprotein cholesterol had no significant differences among these three groups (P > 0.05). Conclusions: The levels of plasma lipids in patient with RA are abnormal,which is related to inflammation activity.
2011, 36(2): 158-160.
Abstract:
Objective: To observe the clinical effect of oxygen atomized inhalation of pulmicort repule ambroxol in treatment of infants with bronchiolitis. Methods: Ninety-three infants with bronchopneumonia were randomly divided into two groups. The treatment group (48 cases) were administered oxygen atomized inhalation of pulmicort repule 0.5 mg,ambloxoi 15 mg and NS 2 ml twice a day,while the control group (45 cases) were treated with ultrasonic atomized inhalation of α-chymotrpsin 3 mg,DXM 2 mg and NS 20 ml twice a day. The clinical symptoms were observed. Results: The disappearing time of the clinical symptoms,wheezing rale and the mean therapy time of the treatment group were shorter than those of the control group (P < 0.05-P < 0.01). The overall response rates of the treatment group and the control group were 80.00% and 93.75%, respectively. The difference was significant (P < 0.01). Conclusions: The therapy of oxygen atomized inhalation of pulmicort repule and ambroxol is effective on infants with bronchiolitis,and is worth spreading
Objective: To observe the clinical effect of oxygen atomized inhalation of pulmicort repule ambroxol in treatment of infants with bronchiolitis. Methods: Ninety-three infants with bronchopneumonia were randomly divided into two groups. The treatment group (48 cases) were administered oxygen atomized inhalation of pulmicort repule 0.5 mg,ambloxoi 15 mg and NS 2 ml twice a day,while the control group (45 cases) were treated with ultrasonic atomized inhalation of α-chymotrpsin 3 mg,DXM 2 mg and NS 20 ml twice a day. The clinical symptoms were observed. Results: The disappearing time of the clinical symptoms,wheezing rale and the mean therapy time of the treatment group were shorter than those of the control group (P < 0.05-P < 0.01). The overall response rates of the treatment group and the control group were 80.00% and 93.75%, respectively. The difference was significant (P < 0.01). Conclusions: The therapy of oxygen atomized inhalation of pulmicort repule and ambroxol is effective on infants with bronchiolitis,and is worth spreading
2011, 36(2): 161-162,165.
Abstract:
Objective: To discuss the advantages and disadvantages of minimal thoracoabdominal incision without disconnecting the costal arch and the traditional incision in treatment of thoracoabdominal injuries. Methods: The operation time,hospital stay,out-of-bed time and postoperative complications between 34 cases treated by thoracoabdominal incision without disconnecting the costal arch (test group) and 26 cases by traditional incision (control group) were compared and analyzed. Results: The operation time,hospital stay,out-of-bed time of the test group were obviously less than that of the control group (P < 0.01). The postoperative complications of the test group were less than the control group (P < 0.01). Conclusions: The minimal thoracoabdominal incision without disconnecting the costal arch is superior to the traditional incision in treatment of thoracoabdominal injuries,which is advantageous to the recovery and rehabilitation of the patients.
Objective: To discuss the advantages and disadvantages of minimal thoracoabdominal incision without disconnecting the costal arch and the traditional incision in treatment of thoracoabdominal injuries. Methods: The operation time,hospital stay,out-of-bed time and postoperative complications between 34 cases treated by thoracoabdominal incision without disconnecting the costal arch (test group) and 26 cases by traditional incision (control group) were compared and analyzed. Results: The operation time,hospital stay,out-of-bed time of the test group were obviously less than that of the control group (P < 0.01). The postoperative complications of the test group were less than the control group (P < 0.01). Conclusions: The minimal thoracoabdominal incision without disconnecting the costal arch is superior to the traditional incision in treatment of thoracoabdominal injuries,which is advantageous to the recovery and rehabilitation of the patients.
2011, 36(2): 163-165.
Abstract:
Objective: To evaluate the clinical value of retroperitoneal laparoscopic ureterolithotomy for the treatment of impacted upper ureteral calculi. Methods: The clinical data of 21 cases by retroperitoneal laparoscopic ureterolithotomy (retroperitoneal laparoscopic group) and 27 cases by open ureterolithotomy (open surgical group) for the treatment of impacted upper ureteral calculi were retrospectively analyzed and compared. Results: All the 48 cases were treated successfully with no serious complication. Compared with open surgical group,intraoperative blood loss,the time of intestinal functional recovery,out-of-bed action and hospital stay after operation were significantly better than those in retroperitoneal laparoscopic group (P < 0.01), the time of operation, the time of drainage-tube and urinary catheter removed after operation had no significant difference between these two groups (P > 0.05). Conclusions: Retroperitoneal laparoscopic ureterolithotomy is safe, minimally invasive and effective. It may be the first choice for treatment of impacted upper ureteral calculi.
Objective: To evaluate the clinical value of retroperitoneal laparoscopic ureterolithotomy for the treatment of impacted upper ureteral calculi. Methods: The clinical data of 21 cases by retroperitoneal laparoscopic ureterolithotomy (retroperitoneal laparoscopic group) and 27 cases by open ureterolithotomy (open surgical group) for the treatment of impacted upper ureteral calculi were retrospectively analyzed and compared. Results: All the 48 cases were treated successfully with no serious complication. Compared with open surgical group,intraoperative blood loss,the time of intestinal functional recovery,out-of-bed action and hospital stay after operation were significantly better than those in retroperitoneal laparoscopic group (P < 0.01), the time of operation, the time of drainage-tube and urinary catheter removed after operation had no significant difference between these two groups (P > 0.05). Conclusions: Retroperitoneal laparoscopic ureterolithotomy is safe, minimally invasive and effective. It may be the first choice for treatment of impacted upper ureteral calculi.
2011, 36(2): 166-167.
Abstract:
Objective: To evaluate the clinical efficacy of retroperitoneal anatomical laparoscopy on adrenal tumorectomy. Methods: A total of six patients were operate on retroperitoneal anatomical laparoscopic adrenal tumorectomy. Results: Five cases were successful, one case converted to open surgery for excessive bleeding. The operation time was ranged from 60 min to 150 min. All patients recovered well with no obvious complications. Conclusions: Retroperitoneal anatomical laparoscopic adrenal tumorectomy has more advantages for clear operation field,less bleeding and complications. It could be a standard surgery option for adrenal tumor.
Objective: To evaluate the clinical efficacy of retroperitoneal anatomical laparoscopy on adrenal tumorectomy. Methods: A total of six patients were operate on retroperitoneal anatomical laparoscopic adrenal tumorectomy. Results: Five cases were successful, one case converted to open surgery for excessive bleeding. The operation time was ranged from 60 min to 150 min. All patients recovered well with no obvious complications. Conclusions: Retroperitoneal anatomical laparoscopic adrenal tumorectomy has more advantages for clear operation field,less bleeding and complications. It could be a standard surgery option for adrenal tumor.
2011, 36(2): 171-173.
Abstract:
Objective: To evaluate diastole function of left ventricular in early diabetic cardiomyopathy by two-dimensional strain echocardiography (2-DSE). Methods: Twenty-nine diabetic patients and 30 healthy people were chosed,strain rate based on Doppler tissue imaging and 2-DSE were performed on DM and healthy subjects at apical long axis,two-chamber and four-chamber views. The following parameters were analyzed in basal and middle segments (all in longitudinal direction only):early diastolic strain rate (SRe), late diastolic strain rate (SRa) and the ratio of SRe/SRa; mitral Doppler inflow were analyzed and E/A ratio were calculated. The results were compared. Results: Compared with healthy subjects,the parameters of E,E/A,SRe,SRe/Sra,in DM decreased significantly (P < 0.01),there was a significant difference in those parameters of SRe,SRe/SRa between two groups. Conclusions: 2-DSE is a new simple,rapid,angle-independent and reproducible method to assess global and regional left ventricular myocardial diastole function quanlitification. It can assess early left ventricular diastolic dysfunction in diabetic cardiomyopathy.
Objective: To evaluate diastole function of left ventricular in early diabetic cardiomyopathy by two-dimensional strain echocardiography (2-DSE). Methods: Twenty-nine diabetic patients and 30 healthy people were chosed,strain rate based on Doppler tissue imaging and 2-DSE were performed on DM and healthy subjects at apical long axis,two-chamber and four-chamber views. The following parameters were analyzed in basal and middle segments (all in longitudinal direction only):early diastolic strain rate (SRe), late diastolic strain rate (SRa) and the ratio of SRe/SRa; mitral Doppler inflow were analyzed and E/A ratio were calculated. The results were compared. Results: Compared with healthy subjects,the parameters of E,E/A,SRe,SRe/Sra,in DM decreased significantly (P < 0.01),there was a significant difference in those parameters of SRe,SRe/SRa between two groups. Conclusions: 2-DSE is a new simple,rapid,angle-independent and reproducible method to assess global and regional left ventricular myocardial diastole function quanlitification. It can assess early left ventricular diastolic dysfunction in diabetic cardiomyopathy.
2011, 36(2): 174-175.
Abstract:
Objective: To explore the value of color doppler ultrasonography in diagnosis of breast cancer. Methods: One hundred and twenty-five patients with breast cancer were examined by doppler ultrasonography. The edge shape,internal echo,back echo,surrounding tissues and color doppler flow imaging were observed and analyzed. Results: One hundred and twenty cases were correctly diagnosed among 125 patients by postoperative pathology section. The diagnosis corresponding rate was 96.0%. The features of typical breast cancer included irregular profile,no obvious echo envelope,posteriorecho attenuation,grit calcification,and length and width diameter proportionality >1; but mastocarcinoma had no above features. Conclusions: Ultrasonic wave is of high value in diagnosis of breast cancer.
Objective: To explore the value of color doppler ultrasonography in diagnosis of breast cancer. Methods: One hundred and twenty-five patients with breast cancer were examined by doppler ultrasonography. The edge shape,internal echo,back echo,surrounding tissues and color doppler flow imaging were observed and analyzed. Results: One hundred and twenty cases were correctly diagnosed among 125 patients by postoperative pathology section. The diagnosis corresponding rate was 96.0%. The features of typical breast cancer included irregular profile,no obvious echo envelope,posteriorecho attenuation,grit calcification,and length and width diameter proportionality >1; but mastocarcinoma had no above features. Conclusions: Ultrasonic wave is of high value in diagnosis of breast cancer.
2011, 36(2): 176-178.
Abstract:
Objective: To investigate Klebsiella pneumoniae infection in neonatal ward and its drug resistance so as to provide basis for rational use of antibiotics. Methods: Klebsiella pneumonia isolated by ATBTM New biochemical identification system in neonatal ward from Jun. 2008 to Jun. 2010 and the drug resistance (determined by K-B) were analyzed retrospectively. Results: A total of 123 strains of Klebsiella pneumoniae were isolated from the samples. Of them,76 were isolated from sputum and throat swab specimens,18 from tracheal intubations,15 from blood samples,9 from the urine and 5 from other specimens. Klebsiella pneumoniae was highly resistant to cephalosporin antibiotics and sensitive to imipenem and cephalosporins containing enzyme inhibitor. The rate of extendedspectrum β-lactamases (ESBLs) producing strains in Klebsiella pneumoniae was 60.9%. Conclusions: Klebsiella pneumoniae in neonatal ward produces high ESBLs, and is highly resistant to commonly used antimicrobial, presenting multidrug resistance, which should arouse the attention of the clinicians. Imipenem and cephalosporin containing inhibitors can be selected as the first choice for treatment of neonatal Klebsiella pneumoniae.
Objective: To investigate Klebsiella pneumoniae infection in neonatal ward and its drug resistance so as to provide basis for rational use of antibiotics. Methods: Klebsiella pneumonia isolated by ATBTM New biochemical identification system in neonatal ward from Jun. 2008 to Jun. 2010 and the drug resistance (determined by K-B) were analyzed retrospectively. Results: A total of 123 strains of Klebsiella pneumoniae were isolated from the samples. Of them,76 were isolated from sputum and throat swab specimens,18 from tracheal intubations,15 from blood samples,9 from the urine and 5 from other specimens. Klebsiella pneumoniae was highly resistant to cephalosporin antibiotics and sensitive to imipenem and cephalosporins containing enzyme inhibitor. The rate of extendedspectrum β-lactamases (ESBLs) producing strains in Klebsiella pneumoniae was 60.9%. Conclusions: Klebsiella pneumoniae in neonatal ward produces high ESBLs, and is highly resistant to commonly used antimicrobial, presenting multidrug resistance, which should arouse the attention of the clinicians. Imipenem and cephalosporin containing inhibitors can be selected as the first choice for treatment of neonatal Klebsiella pneumoniae.
2011, 36(2): 181-182,186.
Abstract:
Objective: To survey the grateful disposition in college students and to analyze the related factors. Methods: One thousand three hundreds college students in four colleges in one city were investigated with a self-designed questionnaire including sociology of population data and Gratitude Questionnaire 6. Results: The scores were significantly different in students of different gender, birthplaces,whether or not being the only child in the family,and students in the medical college and other colleges (P < 0.01); no significant difference was observed in the students having or having not accepted aids (P > 0.05). The grateful disposition was related to the gender,number of children in the family,mother's education level and family income (P < 0.05 to P < 0.01),but not to father's education level (P > 0.05). Conclusions: The grateful disposition of the college students is influenced by the family,school and society.
Objective: To survey the grateful disposition in college students and to analyze the related factors. Methods: One thousand three hundreds college students in four colleges in one city were investigated with a self-designed questionnaire including sociology of population data and Gratitude Questionnaire 6. Results: The scores were significantly different in students of different gender, birthplaces,whether or not being the only child in the family,and students in the medical college and other colleges (P < 0.01); no significant difference was observed in the students having or having not accepted aids (P > 0.05). The grateful disposition was related to the gender,number of children in the family,mother's education level and family income (P < 0.05 to P < 0.01),but not to father's education level (P > 0.05). Conclusions: The grateful disposition of the college students is influenced by the family,school and society.
2011, 36(2): 183-186.
Abstract:
Objective: To investigate the association of subjective well-being with self-concordance and personality of the medical graduates. Methods: A total of 332 medical graduates were surveyed by General Well-Being Schedule,Self Consistency and Congruence Scale,and Eysenck Personality Questionnaire. Results: The scores of the average general well-being turned out to be 79.76 ±12.76 and self-consistency 89.56 ±13.41; differences existed in students of different genders and different grades (P < 0.05 to P < 0.01); no distinct differences were observed in personality types (P > 0.05). Self-stereotypy,lack of self-experiences,psychoticism and nervosity were negatively related to the general well-being (P < 0.01),while self-flexibility and extroverted personality were positively related to the general well-being (P < 0.01). Conclusions: Self-flexibility and stable personality trait are important factors affecting the general well-being of the medical graduates.
Objective: To investigate the association of subjective well-being with self-concordance and personality of the medical graduates. Methods: A total of 332 medical graduates were surveyed by General Well-Being Schedule,Self Consistency and Congruence Scale,and Eysenck Personality Questionnaire. Results: The scores of the average general well-being turned out to be 79.76 ±12.76 and self-consistency 89.56 ±13.41; differences existed in students of different genders and different grades (P < 0.05 to P < 0.01); no distinct differences were observed in personality types (P > 0.05). Self-stereotypy,lack of self-experiences,psychoticism and nervosity were negatively related to the general well-being (P < 0.01),while self-flexibility and extroverted personality were positively related to the general well-being (P < 0.01). Conclusions: Self-flexibility and stable personality trait are important factors affecting the general well-being of the medical graduates.
Establishment and application of multimedia teaching system in the morphological experimental course
2011, 36(2): 187-189.
Abstract:
Objective: To establish and utilize multimedia teaching system in the experimental course centre of morphylogy. Methods: The experimental resourses were integrated to set up the multimedia teaching system in the experimental course. The methods of between multimedia and traditional teaching were compared by questionnaire in the experimental teaching of morphyology. Results: Ninety point four percent students approved the use of the multimedia system in the experimental teaching. Multimedia teaching method significantly increased the students' attention, understanding, amount of information and learning interest than the traditional teaching method. Conclusions: Multimedia system in experimental teaching was established successfully,utilized extensively and better than the method of traditional teaching.
Objective: To establish and utilize multimedia teaching system in the experimental course centre of morphylogy. Methods: The experimental resourses were integrated to set up the multimedia teaching system in the experimental course. The methods of between multimedia and traditional teaching were compared by questionnaire in the experimental teaching of morphyology. Results: Ninety point four percent students approved the use of the multimedia system in the experimental teaching. Multimedia teaching method significantly increased the students' attention, understanding, amount of information and learning interest than the traditional teaching method. Conclusions: Multimedia system in experimental teaching was established successfully,utilized extensively and better than the method of traditional teaching.
2011, 36(2): 192-194.
Abstract:
Objective: To investigate the efficacy of health belief model on self-care behavior of the diabetic patients. Methods: A selfcontrol observation based-on health belief theory was carried out in 65 patients with type 2 diabetes. The diabetes knowledge,self-care ability and blood glucose control of the patients were compared before and after intervention. Results: After intervention,the patients' knowledge about diabetes,ability of diet control,regular exercise,medical order compliance,foot care and preventive management of high and low blood glucose were enhanced,and their blood sugar level improved significantly (P < 0.01),while their self-monitoring ability presented no significant difference before and after intervention (P > 0.05). Conclusions: Health belief model can improve the efficiency of diabetes healthy education as well as the self-care behavior of the diabetic patients.
Objective: To investigate the efficacy of health belief model on self-care behavior of the diabetic patients. Methods: A selfcontrol observation based-on health belief theory was carried out in 65 patients with type 2 diabetes. The diabetes knowledge,self-care ability and blood glucose control of the patients were compared before and after intervention. Results: After intervention,the patients' knowledge about diabetes,ability of diet control,regular exercise,medical order compliance,foot care and preventive management of high and low blood glucose were enhanced,and their blood sugar level improved significantly (P < 0.01),while their self-monitoring ability presented no significant difference before and after intervention (P > 0.05). Conclusions: Health belief model can improve the efficiency of diabetes healthy education as well as the self-care behavior of the diabetic patients.
2011, 36(2): 195-197.
Abstract:
Objective: To observe the clinical effect of nursing intervention on the parturients in the labor with nuchal cord. Methods: One hundred and eighty parturients in labor with nuchal cord were divided into observation group and control group. The control group was given general nursing and necessary psychological intervention. The observation group used another temporary nursing before childbirth. Then outcomes of mother and infant were compared. Results: The rates of cesarean section and neonatal asphyxia in observation group were significantly lower than in control group (P < 0.01). Conclusions: The nursing intervention has a good clinical effect on the prognosis of mather and infant when the parturients in the labor with nuchal cord.
Objective: To observe the clinical effect of nursing intervention on the parturients in the labor with nuchal cord. Methods: One hundred and eighty parturients in labor with nuchal cord were divided into observation group and control group. The control group was given general nursing and necessary psychological intervention. The observation group used another temporary nursing before childbirth. Then outcomes of mother and infant were compared. Results: The rates of cesarean section and neonatal asphyxia in observation group were significantly lower than in control group (P < 0.01). Conclusions: The nursing intervention has a good clinical effect on the prognosis of mather and infant when the parturients in the labor with nuchal cord.
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