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2012 Vol. 37, No. 11
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2012, 36(11): 1273-1276.
Abstract:
Objective: To construct human osteoinductive factor(OIF) recombinant lentivirus vector and over-express OIF stably in human aortic smooth muscle cells(HASMCs) . Methods: OIF-3FLAG was amplified by PCR and cloned into vector(pMSCV PIG) . The lentivirus vector pMSCV PIG-OIF-3FLAG was constructed and indentified by sequencing. pMSCV PIG-OIF-3FLAG or pMSCV PIG with helper vectors of VSVG and GAG-POL were co-transfected into 293T cells. Infectious lentivirus was harvested at 48 h post-transfection. The lentivirus was added in HASMCs. After 48 h, puromycin was added for 4 d. When the cells reached 90%, HASMCs over-expressed pMSCV PIG-OIF-3FLAG stably. Human OIF expression was verified by quantitative real-time PCR and western blot analysis. Results: The lentivirus vector pMSCV PIG-OIF-3FLAG was successfully constructed and confirmed by enzyme digestion and sequencing. HASMCs were infected by lentivirus supernatant. Over-expression of OIF in HASMCs was revealed by real-time PCR and western blot. Conclusions: Human OIF gene recombined lentivirus vector and a cell model which over-expessing OIF stably in HASMCs were successfully established.
Objective: To construct human osteoinductive factor(OIF) recombinant lentivirus vector and over-express OIF stably in human aortic smooth muscle cells(HASMCs) . Methods: OIF-3FLAG was amplified by PCR and cloned into vector(pMSCV PIG) . The lentivirus vector pMSCV PIG-OIF-3FLAG was constructed and indentified by sequencing. pMSCV PIG-OIF-3FLAG or pMSCV PIG with helper vectors of VSVG and GAG-POL were co-transfected into 293T cells. Infectious lentivirus was harvested at 48 h post-transfection. The lentivirus was added in HASMCs. After 48 h, puromycin was added for 4 d. When the cells reached 90%, HASMCs over-expressed pMSCV PIG-OIF-3FLAG stably. Human OIF expression was verified by quantitative real-time PCR and western blot analysis. Results: The lentivirus vector pMSCV PIG-OIF-3FLAG was successfully constructed and confirmed by enzyme digestion and sequencing. HASMCs were infected by lentivirus supernatant. Over-expression of OIF in HASMCs was revealed by real-time PCR and western blot. Conclusions: Human OIF gene recombined lentivirus vector and a cell model which over-expessing OIF stably in HASMCs were successfully established.
2012, 36(11): 1277-1279,1282.
Abstract:
Objective: To investigate the growth inhibition role of arsenic trioxide(As2O3) on human amalignant melanoma A375 cell, and observe whether Caspase-3 protein can be expressed in A375 cell after induced by As2O3 . To provide theory and experiment base for treament of malignant melanoma. Methods: The morphologic changes of A375 cells were detected by fluorescence staining, apoptosis rate of A375 cells was detected with flow cytometry. Caspase-3 protein was detected by immunehistochemistry in A375 cells. The expression of Survivin mRNA was examined by semi-quantitative RT-PCR. Results: As2O3 caused morphologic change of A375 cells by fluorescence staining. As2O3 promoted A375 cell apoptosis significantly. When A375 cells were treated with As2O3 in 5, 10, and 20 mol/L for 24 hours, the apoptosis rate was 7. 07%, 38. 66% and 4. 34%. Caspase-3 protein was expressed positively in A375 cells by co-culture with As2O3 . The expression of Survivin mRNA was inhibited by As2O3 . Conclusions: As2O3 can cause morphologic change of A375 cells and promote apoptosis happening. As2O3 can induce A375 cells to express Caspase-3 protein. As2O3 can inhibit Survivin protein expression in A375 cells.
Objective: To investigate the growth inhibition role of arsenic trioxide(As2O3) on human amalignant melanoma A375 cell, and observe whether Caspase-3 protein can be expressed in A375 cell after induced by As2O3 . To provide theory and experiment base for treament of malignant melanoma. Methods: The morphologic changes of A375 cells were detected by fluorescence staining, apoptosis rate of A375 cells was detected with flow cytometry. Caspase-3 protein was detected by immunehistochemistry in A375 cells. The expression of Survivin mRNA was examined by semi-quantitative RT-PCR. Results: As2O3 caused morphologic change of A375 cells by fluorescence staining. As2O3 promoted A375 cell apoptosis significantly. When A375 cells were treated with As2O3 in 5, 10, and 20 mol/L for 24 hours, the apoptosis rate was 7. 07%, 38. 66% and 4. 34%. Caspase-3 protein was expressed positively in A375 cells by co-culture with As2O3 . The expression of Survivin mRNA was inhibited by As2O3 . Conclusions: As2O3 can cause morphologic change of A375 cells and promote apoptosis happening. As2O3 can induce A375 cells to express Caspase-3 protein. As2O3 can inhibit Survivin protein expression in A375 cells.
2012, 36(11): 1280-1282.
Abstract:
Objective: To build a stereotactic data set and internal surface regression equation of cerebral parietooccipital sulcus. Methods: The cross-sectional MRI data of 30 normal adult were imported into Photoshop by form transition. Through the strict registration of the image, the coordinate system of the software coincide with the system based on AC-PC line were made. The X and Y values of sample points with per 3 mm of the X-axis which began from the most inside point of the parietooccipital sulcus and along the parietooccipital sulcus line were measured and recorded and the multiplication of the thickness and number of the layer acted as Z values. All data of sample points of the parietooccipital sulcus were collected and analyzed by SPSS 16. 0 software, and plane equation was solved. Results: The three-dimensional stereotactic data set and internal surface regression equation of the pareitooccipital sulcus were constructed. In sagittal plane, the straight line regression equations of Z toward Y were 0. 774(Z) and 26. 975(Y) in right side and 0. 760(Z) and 28. 599(Y) in left side. Conclusions: The stereotactic data set and internal surfacicial regression equation of the pareitooccipital sulcus are constructed in the three-dimensional coordinates, which can play an important role in the construction of stereotactic surgery, and provide anatomical basis for interventional radiologic treatment.
Objective: To build a stereotactic data set and internal surface regression equation of cerebral parietooccipital sulcus. Methods: The cross-sectional MRI data of 30 normal adult were imported into Photoshop by form transition. Through the strict registration of the image, the coordinate system of the software coincide with the system based on AC-PC line were made. The X and Y values of sample points with per 3 mm of the X-axis which began from the most inside point of the parietooccipital sulcus and along the parietooccipital sulcus line were measured and recorded and the multiplication of the thickness and number of the layer acted as Z values. All data of sample points of the parietooccipital sulcus were collected and analyzed by SPSS 16. 0 software, and plane equation was solved. Results: The three-dimensional stereotactic data set and internal surface regression equation of the pareitooccipital sulcus were constructed. In sagittal plane, the straight line regression equations of Z toward Y were 0. 774(Z) and 26. 975(Y) in right side and 0. 760(Z) and 28. 599(Y) in left side. Conclusions: The stereotactic data set and internal surfacicial regression equation of the pareitooccipital sulcus are constructed in the three-dimensional coordinates, which can play an important role in the construction of stereotactic surgery, and provide anatomical basis for interventional radiologic treatment.
2012, 36(11): 1283-1286.
Abstract:
Objective: To evaluate the feasibility of non-woven silk fibroin net as a biocompatible scaffold of the auricular chondrocytes for construction of tissue-engineered cartilage. Methods: Chondrocytes were harvested from auricular cartilage by trypsin and type Ⅱ collagenase digestion, and expanded in culture flasks. The chondrocytes(P1) were seeded into non-woven silk fibroin nets, and incubated in CO2 incubator. The adhesion, proliferation and growth of cells were observed under inverted microscope every day and the microstructure of the constructs was observed by using scanning electron microscope(SEM) at 3, 7 and 10 d after coculture. The constructs were harvested and analyzed by histology at 1, 2 , 4 and 6 weeks after coculture, and the mRNA expression of type Ⅱ collagen was examined using RT-PCR. Results: The scaffold was covered with chondrocytes at 48 h and these cells began to secrete extracellular matrix(ECM) at 72 h after coculture. The surface of scaffold was covered with chondrocytes and ECM under SEM. Stent shallow already had some cartilage tissue and a little lacunae at 2 weeks. The cartilage tissue thickened and lacunae increased, but the certer of stent had a little cells, which were star or spindle type and secreted a little ECM at 4 and 6 weeks. The mRNA expression of type Ⅱ collagen was detected by RT-PCR at all time-points. Conclusions: Cartilage-like tissues can form at non-woven silk fibroin net with the auricular chondrocytes, but culture conditions should be further optimized.
Objective: To evaluate the feasibility of non-woven silk fibroin net as a biocompatible scaffold of the auricular chondrocytes for construction of tissue-engineered cartilage. Methods: Chondrocytes were harvested from auricular cartilage by trypsin and type Ⅱ collagenase digestion, and expanded in culture flasks. The chondrocytes(P1) were seeded into non-woven silk fibroin nets, and incubated in CO2 incubator. The adhesion, proliferation and growth of cells were observed under inverted microscope every day and the microstructure of the constructs was observed by using scanning electron microscope(SEM) at 3, 7 and 10 d after coculture. The constructs were harvested and analyzed by histology at 1, 2 , 4 and 6 weeks after coculture, and the mRNA expression of type Ⅱ collagen was examined using RT-PCR. Results: The scaffold was covered with chondrocytes at 48 h and these cells began to secrete extracellular matrix(ECM) at 72 h after coculture. The surface of scaffold was covered with chondrocytes and ECM under SEM. Stent shallow already had some cartilage tissue and a little lacunae at 2 weeks. The cartilage tissue thickened and lacunae increased, but the certer of stent had a little cells, which were star or spindle type and secreted a little ECM at 4 and 6 weeks. The mRNA expression of type Ⅱ collagen was detected by RT-PCR at all time-points. Conclusions: Cartilage-like tissues can form at non-woven silk fibroin net with the auricular chondrocytes, but culture conditions should be further optimized.
2012, 36(11): 1287-1288,1293.
Abstract:
Objective: To investigate the changes of plasma interleukin-17A(IL-17A) level in patients with systemic lupus erythematosus(SLE) and analyze the clinical implication. Methods: Plasma IL-17A level was measured by ELISA from 60 SLE patients(13 without glucocorticoid treatment and 47 with glucocorticoid treatment) and 29 healthy persons. The relationship between IL-17A and clinical or laboratory parameters of SLE patients was explored. Results: IL-17A levels were elevated significantly in SLE patients without glucocorticoid treatment than in SLE patients with glucocorticoid treatment or healthy persons(P ＜ 0. 05-P ＜ 0. 01) . The negative correlation was observed between IL-17A expression and C4, erythrocyte sedimentation rate(P ＜ 0. 05) , but no correlation with C3, IgG, anti ds-DNA antibody, SLE disease activity index score and 24 h urine protein excretion(P ＞ 0. 05) . Conclusions: The level of plasma IL-17A in SLE patient without glucocorticoid treatment was higher than in normal control. IL-17A might play an important role in the pathogenesis of SLE. Treatment with glucocorticoid can decrease IL-17A level in SLE significantly.
Objective: To investigate the changes of plasma interleukin-17A(IL-17A) level in patients with systemic lupus erythematosus(SLE) and analyze the clinical implication. Methods: Plasma IL-17A level was measured by ELISA from 60 SLE patients(13 without glucocorticoid treatment and 47 with glucocorticoid treatment) and 29 healthy persons. The relationship between IL-17A and clinical or laboratory parameters of SLE patients was explored. Results: IL-17A levels were elevated significantly in SLE patients without glucocorticoid treatment than in SLE patients with glucocorticoid treatment or healthy persons(P ＜ 0. 05-P ＜ 0. 01) . The negative correlation was observed between IL-17A expression and C4, erythrocyte sedimentation rate(P ＜ 0. 05) , but no correlation with C3, IgG, anti ds-DNA antibody, SLE disease activity index score and 24 h urine protein excretion(P ＞ 0. 05) . Conclusions: The level of plasma IL-17A in SLE patient without glucocorticoid treatment was higher than in normal control. IL-17A might play an important role in the pathogenesis of SLE. Treatment with glucocorticoid can decrease IL-17A level in SLE significantly.
2012, 36(11): 1289-1293.
Abstract:
Objective: To study the risk factors of osteoporosis(OP) in female patients with rheumatoid arthritis(RA) , and the related clinical factors. Methods: The bone mineral density(BMD) of the lumbar vertebrae(L1-L4) , femoral neck and total femoral of 115 female patients with RA and 101 female healthy subjects area were measured by dual energy X-ray absorptiometry; and the clinical and laboratory indexes were observed in the meantime. Results: The incidence of OP in female patients with RA was significant higher than that in healthy subjects(P ＜ 0. 01) . The BMD of all positions in female patients with RA was lower than that in healthy subjects(P ＜ 0. 05-P ＜ 0. 01) . The RA patients diagnosed as having OP had lower BMD than those having not(P ＜ 0. 01) . The former had elder ages, longer years of menopause, longer duration of disease and morning stiffness, tender joint counts, higher HAQ scores and worse function of joint and X-ray status than the latter(P ＜ 0. 05-P ＜ 0. 01) . The osteoporotic fracture probability in patients with RA was obviously higher than that in healthy subjects in the next 10 years(P ＜ 0. 01) . The BMD of L1-L4 in female patients with RA who were taking corticosteroid was lower than that of patients taking no corticosteroid(P ＜ 0. 05) . Logistic regression analysis showed that menopause[OR = 4. 582(1. 503-13. 974) , P ＜ 0. 01］and X-ray status[OR = 2. 267(1. 233-4. 167) , P ＜ 0. 01) were the main risk factors for the OP in female patients with RA. Conclusions: Compared with the healthy subjects, the incidence of OP and the risk of osteoporotic fracture in female patients with RA rise apparently. The occurrence of OP is related with several risk factors, such as menopause, worse X-ray status and taking corticosteroid.
Objective: To study the risk factors of osteoporosis(OP) in female patients with rheumatoid arthritis(RA) , and the related clinical factors. Methods: The bone mineral density(BMD) of the lumbar vertebrae(L1-L4) , femoral neck and total femoral of 115 female patients with RA and 101 female healthy subjects area were measured by dual energy X-ray absorptiometry; and the clinical and laboratory indexes were observed in the meantime. Results: The incidence of OP in female patients with RA was significant higher than that in healthy subjects(P ＜ 0. 01) . The BMD of all positions in female patients with RA was lower than that in healthy subjects(P ＜ 0. 05-P ＜ 0. 01) . The RA patients diagnosed as having OP had lower BMD than those having not(P ＜ 0. 01) . The former had elder ages, longer years of menopause, longer duration of disease and morning stiffness, tender joint counts, higher HAQ scores and worse function of joint and X-ray status than the latter(P ＜ 0. 05-P ＜ 0. 01) . The osteoporotic fracture probability in patients with RA was obviously higher than that in healthy subjects in the next 10 years(P ＜ 0. 01) . The BMD of L1-L4 in female patients with RA who were taking corticosteroid was lower than that of patients taking no corticosteroid(P ＜ 0. 05) . Logistic regression analysis showed that menopause[OR = 4. 582(1. 503-13. 974) , P ＜ 0. 01］and X-ray status[OR = 2. 267(1. 233-4. 167) , P ＜ 0. 01) were the main risk factors for the OP in female patients with RA. Conclusions: Compared with the healthy subjects, the incidence of OP and the risk of osteoporotic fracture in female patients with RA rise apparently. The occurrence of OP is related with several risk factors, such as menopause, worse X-ray status and taking corticosteroid.
2012, 36(11): 1294-1295,1297.
Abstract:
Objective: To investigate the endoscopic and pathologic characteristics of 52 cases with the serrated adenoma of large intestine. Methods: The endoscopic and pathologic characteristics of 52 cases with the serrated adenoma of large intestine were retrospectively analysed. Results: Among 52 cases with serrated adenoma, there were 19 cases in rectum, 13 cases in sigmoid colon, 2 cases in the junction of rectum and sigmoid colon, 6 cases in descending colon, 8 cases in transverse colon, 3 cases in ascending colon and 1 case in cecum. One case with multiple adenoma and 51 cases with single adenoma were found, which diameters were 1 to 25 mm. Fifty-five cases with serrated adenoma included 32 cases with wide base polyps, 15 cases with sub-pedicle polyps and 5 cases with pedicle polyps. The pathologic examination showed 17 cases with pure serrated adenoma, 33 cases with serrated adenoma complicated with low grade intraepithelial neoplasia, 1 case with serrated adenoma complicated with high grade intraepithelial neoplasia and 1 case with local malignant of serrated adenoma. Conclusions: The clinical symptoms of serrated adenoma are non-specificity, the single and wide base polyps complicated with intraepithelial neoplasia are most, located in rectum.
Objective: To investigate the endoscopic and pathologic characteristics of 52 cases with the serrated adenoma of large intestine. Methods: The endoscopic and pathologic characteristics of 52 cases with the serrated adenoma of large intestine were retrospectively analysed. Results: Among 52 cases with serrated adenoma, there were 19 cases in rectum, 13 cases in sigmoid colon, 2 cases in the junction of rectum and sigmoid colon, 6 cases in descending colon, 8 cases in transverse colon, 3 cases in ascending colon and 1 case in cecum. One case with multiple adenoma and 51 cases with single adenoma were found, which diameters were 1 to 25 mm. Fifty-five cases with serrated adenoma included 32 cases with wide base polyps, 15 cases with sub-pedicle polyps and 5 cases with pedicle polyps. The pathologic examination showed 17 cases with pure serrated adenoma, 33 cases with serrated adenoma complicated with low grade intraepithelial neoplasia, 1 case with serrated adenoma complicated with high grade intraepithelial neoplasia and 1 case with local malignant of serrated adenoma. Conclusions: The clinical symptoms of serrated adenoma are non-specificity, the single and wide base polyps complicated with intraepithelial neoplasia are most, located in rectum.
2012, 36(11): 1296-1297.
Abstract:
Objective: To study the clinical significance of preserving intercostobrachial nerve(ICBN) during the axillary lymph nodes excision in breast cancer operation. Methods: Sixty-one patients with stages Ⅰ and Ⅱ breast cancer were divided into treatment group(30 cases) and control group(31 cases) . ICBN was regularly resected in operation in control group, ICBN was preserved in axillary lymph node dissection operation in treatment group. Patients in both groups were treated as breast cancer regular therapy and followed rehabiliation exercise after operation. Results: The incidece of abnormal sense on medial upper arm in one, three and six months after operation was 16. 7%, 6. 7%, and 6. 7%, respectively, in treatment group, which was significantly lower than that 77. 4%, 64. 5%, and 58. 1%, respectively, in control group(P ＜ 0. 01) . Conclusions: Preserving ICBN in the axillary lymph nodes excision in breast cancer operation can reduce the incidence of abnormal sense on medial upper arm and improve life quality.
Objective: To study the clinical significance of preserving intercostobrachial nerve(ICBN) during the axillary lymph nodes excision in breast cancer operation. Methods: Sixty-one patients with stages Ⅰ and Ⅱ breast cancer were divided into treatment group(30 cases) and control group(31 cases) . ICBN was regularly resected in operation in control group, ICBN was preserved in axillary lymph node dissection operation in treatment group. Patients in both groups were treated as breast cancer regular therapy and followed rehabiliation exercise after operation. Results: The incidece of abnormal sense on medial upper arm in one, three and six months after operation was 16. 7%, 6. 7%, and 6. 7%, respectively, in treatment group, which was significantly lower than that 77. 4%, 64. 5%, and 58. 1%, respectively, in control group(P ＜ 0. 01) . Conclusions: Preserving ICBN in the axillary lymph nodes excision in breast cancer operation can reduce the incidence of abnormal sense on medial upper arm and improve life quality.
2012, 36(11): 1298-1299,1302.
Abstract:
Objective: To evaluate the effect of atorvastatin on pulse pressure in patients with essential hypertension(EH) . Methods: One hundred and eight EH patients were randomly allocated into two groups:common treated group(54 cases) and atorvastatin treated group(54 cases) . Patients in common treated group were accepted common treatment and those in atorvastatin treated group were treated with atorvastatin 40 mg daily besides common treatment. All of them were treated for 12 weeks. Pulse pressure was compared between the two groups before treatment and after 4, 8 , 12 weeks treatment. Results: There was no difference of pulse pressure between two groups before treatment(P ＞ 0. 05) , but was significantly lower in atorvastatin treated group than in common treated group after treatment(P ＜ 0. 01) , and with the treatment prolonging, pulse pressure had an obvious lower tendency in atorvastatin treated group(P ＜ 0. 01) . Conclusions: Atorvastatin could decrease pulse pressure effectively in patients with EH.
Objective: To evaluate the effect of atorvastatin on pulse pressure in patients with essential hypertension(EH) . Methods: One hundred and eight EH patients were randomly allocated into two groups:common treated group(54 cases) and atorvastatin treated group(54 cases) . Patients in common treated group were accepted common treatment and those in atorvastatin treated group were treated with atorvastatin 40 mg daily besides common treatment. All of them were treated for 12 weeks. Pulse pressure was compared between the two groups before treatment and after 4, 8 , 12 weeks treatment. Results: There was no difference of pulse pressure between two groups before treatment(P ＞ 0. 05) , but was significantly lower in atorvastatin treated group than in common treated group after treatment(P ＜ 0. 01) , and with the treatment prolonging, pulse pressure had an obvious lower tendency in atorvastatin treated group(P ＜ 0. 01) . Conclusions: Atorvastatin could decrease pulse pressure effectively in patients with EH.
2012, 36(11): 1300-1302.
Abstract:
Objective: To compare the effects of complete omentum(CO) and partial omentum(PO) resection in subtotal gastrectomy of early gastric cancer. Methods: Forty patients with early gastric cancer undergone with open distal subtotal gastrectomy were randomly divided into CO and PO group(20 patients in each group) . The clinicopathological parameters and the related indexes of postoperative prognosis of two groups were compared. Results: The time of operation and hospital stay of PO group were shorter than that of CO group(P ＜ 0. 01-P ＜ 0. 05) . The serum albumin level of PO group on the first day after operation was higher than that of CO group(P ＜ 0. 05) . There were no differences in the other indexes between the two groups(P ＞ 0. 05) . One case of PO group appeared atelectasis, which was successfully controled by conservative therapy. All patients were alive without recurrence until December 30, 2010. Conclusions: The partial omentum resection in open distal subtotal gastrectomy in the treatment of early gastric cancer has a certain clinical value.
Objective: To compare the effects of complete omentum(CO) and partial omentum(PO) resection in subtotal gastrectomy of early gastric cancer. Methods: Forty patients with early gastric cancer undergone with open distal subtotal gastrectomy were randomly divided into CO and PO group(20 patients in each group) . The clinicopathological parameters and the related indexes of postoperative prognosis of two groups were compared. Results: The time of operation and hospital stay of PO group were shorter than that of CO group(P ＜ 0. 01-P ＜ 0. 05) . The serum albumin level of PO group on the first day after operation was higher than that of CO group(P ＜ 0. 05) . There were no differences in the other indexes between the two groups(P ＞ 0. 05) . One case of PO group appeared atelectasis, which was successfully controled by conservative therapy. All patients were alive without recurrence until December 30, 2010. Conclusions: The partial omentum resection in open distal subtotal gastrectomy in the treatment of early gastric cancer has a certain clinical value.
2012, 36(11): 1303-1304.
Abstract:
Objective: To investigate the clinical effects of hyperextension reduction and the internal fixation of short-segment pedicle bone grafting in treatment of thoracolumbar vertebral compression fractures. Methods: Seventeen vertebral fractures of 15 cases were treated with postural reduction combined with bilateral internal fixation of short-segment vertebral pedicle bone grafting. Cobb's angle measurements, VAS scores and X-ray vertebral height were measured in the preoperative and postoperative, and the end time follow-up. Results: Seventeen vertebral fractures of 15 cases got average follow-up of 10-15 months. The vertebral height and Cobb's angle had significant difference in the preoperative and postoperative(P ＜ 0. 01) and the vertebral height and Cobb' s angle had no significant difference in the postoperative, and the end time follow-up(P ＞ 0. 05) . Conclusions: Treatment of thoracolumbar vertebral compression fractures with hyperextension reduction and the internal fixation of short-segment pedicle bone grafting is a simple and effective way, which can maintain the height of thoracic and lumbar vertebral body and prevent angle deformity of kyphose.
Objective: To investigate the clinical effects of hyperextension reduction and the internal fixation of short-segment pedicle bone grafting in treatment of thoracolumbar vertebral compression fractures. Methods: Seventeen vertebral fractures of 15 cases were treated with postural reduction combined with bilateral internal fixation of short-segment vertebral pedicle bone grafting. Cobb's angle measurements, VAS scores and X-ray vertebral height were measured in the preoperative and postoperative, and the end time follow-up. Results: Seventeen vertebral fractures of 15 cases got average follow-up of 10-15 months. The vertebral height and Cobb's angle had significant difference in the preoperative and postoperative(P ＜ 0. 01) and the vertebral height and Cobb' s angle had no significant difference in the postoperative, and the end time follow-up(P ＞ 0. 05) . Conclusions: Treatment of thoracolumbar vertebral compression fractures with hyperextension reduction and the internal fixation of short-segment pedicle bone grafting is a simple and effective way, which can maintain the height of thoracic and lumbar vertebral body and prevent angle deformity of kyphose.
2012, 36(11): 1305-1306,1309.
Abstract:
Objective: To observe investigate the efficacy and safety of preemptive analgesia with flurbiprofen axetil in preventing postoperative restlessness of patients under general anaesthesia with remifentanil. Methods: One hundred and twenty patients under general anaesthesia with remifentanil were evenly divided into two groups(group F and group C) . Group F were intravenously injected with flurbiprofen axetil 50 mg and group C were injected with placebo(fat emulsion) 5 ml before surgery. The anesthesia awaking time, extubation time and postoperative restlessness were observed in the two groups, and the postoperative analgesia was evaluated by visual analogue scale. Results: When flurbiprofen axetil was used as preemptive analgesia, the postoperative restlessness rate was 5. 0% in group F, which was obviously lower than the 41. 7% in group C(P ＜ 0. 01) . Conclusions: Preemptive analgesia with flurbiprofen axetil can effectively prevent the postoperative restlessness of patients under general anaesthesia with remifentanil.
Objective: To observe investigate the efficacy and safety of preemptive analgesia with flurbiprofen axetil in preventing postoperative restlessness of patients under general anaesthesia with remifentanil. Methods: One hundred and twenty patients under general anaesthesia with remifentanil were evenly divided into two groups(group F and group C) . Group F were intravenously injected with flurbiprofen axetil 50 mg and group C were injected with placebo(fat emulsion) 5 ml before surgery. The anesthesia awaking time, extubation time and postoperative restlessness were observed in the two groups, and the postoperative analgesia was evaluated by visual analogue scale. Results: When flurbiprofen axetil was used as preemptive analgesia, the postoperative restlessness rate was 5. 0% in group F, which was obviously lower than the 41. 7% in group C(P ＜ 0. 01) . Conclusions: Preemptive analgesia with flurbiprofen axetil can effectively prevent the postoperative restlessness of patients under general anaesthesia with remifentanil.
2012, 36(11): 1307-1309.
Abstract:
Objective: To investigate the relationship of the related factors, clinical characteristics, prognosis and severity score of neonatal thrombocytopenia(NT) . Methods: Eighty-nine infants with NT were collected in neonatal intensive care unit, these patients were divided into mild group and severe group. The related factors, treatment, prognosis and relationship with the severity score were analyzed. Results: Compared with mild group, the recovery time of platelet in severe group was prolonged(P ＜ 0. 05) , red blood cell count and severity score were decreased, C reactive protein and incidence of were increased, there were significant differences(P ＜ 0. 05-0. 01) . Conclusions: Infection is the key factor of NT, there is very close relationship between the level of platelet and severity score. Early diagnosis and treatment is very crucial, and most patients have a favorable prognosis.
Objective: To investigate the relationship of the related factors, clinical characteristics, prognosis and severity score of neonatal thrombocytopenia(NT) . Methods: Eighty-nine infants with NT were collected in neonatal intensive care unit, these patients were divided into mild group and severe group. The related factors, treatment, prognosis and relationship with the severity score were analyzed. Results: Compared with mild group, the recovery time of platelet in severe group was prolonged(P ＜ 0. 05) , red blood cell count and severity score were decreased, C reactive protein and incidence of were increased, there were significant differences(P ＜ 0. 05-0. 01) . Conclusions: Infection is the key factor of NT, there is very close relationship between the level of platelet and severity score. Early diagnosis and treatment is very crucial, and most patients have a favorable prognosis.
2012, 36(11): 1310-1311,1314.
Abstract:
Objective: To investigate the effects of holmium laser combined with extracorporeal shock wave lithotripsy(ESWL) by percutaneous nephroscope in treatment of complex renal calculi. Methods: Holmium laser combined with ESWL by percutaneous nephroscope were used to treat 46 cases with complex renal calculi. Results: The mean time of operation and hospital stays were 56 minutes(15-221 min) and 15. 7 days(10-21 d) , and stones clearance was 97. 8%. The severe complications of septic shock and surrounding organs injury were not found. Conclusions: The technology of treatment of complex renal calculi with holmium laser combined with ESWL lithotripsy by percutaneous nephroscope is effective and safe way. It is high clearance rate and less complications, and worthy of clinic application.
Objective: To investigate the effects of holmium laser combined with extracorporeal shock wave lithotripsy(ESWL) by percutaneous nephroscope in treatment of complex renal calculi. Methods: Holmium laser combined with ESWL by percutaneous nephroscope were used to treat 46 cases with complex renal calculi. Results: The mean time of operation and hospital stays were 56 minutes(15-221 min) and 15. 7 days(10-21 d) , and stones clearance was 97. 8%. The severe complications of septic shock and surrounding organs injury were not found. Conclusions: The technology of treatment of complex renal calculi with holmium laser combined with ESWL lithotripsy by percutaneous nephroscope is effective and safe way. It is high clearance rate and less complications, and worthy of clinic application.
2012, 36(11): 1312-1314.
Abstract:
Objective: To observe the clinical effect of budesonide combined with salbutamol atomization inhalation on acute exacerbation phase of chronic obstructive pulmonary disease(AECOPD) . Methods: One hundred patients with AECOPD were randomly divided into two groups:50 patients in control group were injected with dexamethasone(10 mg) intravenously once a day; 50 patients in observed group were treated with budesonide(1 mg) combined with salbutamol(0. 5 ml) atomization inhalation twice a day. The changes of clinical symptom scores, electrolytes levels, blood glucose levels, lung function, blood gas analysis including forced expiratoy volume in the first second, PaO2 and PaCO2 on the first day of 7th day of treatment and the last day before discharge were recorded, meanwhile the side effects were observed. Results: FEV1, PaO2 and PaCO2 in these two groups were improved, and there was significant difference after treatment(P ＜ 0. 05-P ＜ 0. 01) ; the side effect in observed group was significantly less than that in control group. Conclusions: The treatment of budesonide combined with salbutamol atomization inhalation can improve lung function and clinical symptom of patients with AECOPD, which is superior to the effect of intravenous glucocorticoid, and the adverse effect has decreased.
Objective: To observe the clinical effect of budesonide combined with salbutamol atomization inhalation on acute exacerbation phase of chronic obstructive pulmonary disease(AECOPD) . Methods: One hundred patients with AECOPD were randomly divided into two groups:50 patients in control group were injected with dexamethasone(10 mg) intravenously once a day; 50 patients in observed group were treated with budesonide(1 mg) combined with salbutamol(0. 5 ml) atomization inhalation twice a day. The changes of clinical symptom scores, electrolytes levels, blood glucose levels, lung function, blood gas analysis including forced expiratoy volume in the first second, PaO2 and PaCO2 on the first day of 7th day of treatment and the last day before discharge were recorded, meanwhile the side effects were observed. Results: FEV1, PaO2 and PaCO2 in these two groups were improved, and there was significant difference after treatment(P ＜ 0. 05-P ＜ 0. 01) ; the side effect in observed group was significantly less than that in control group. Conclusions: The treatment of budesonide combined with salbutamol atomization inhalation can improve lung function and clinical symptom of patients with AECOPD, which is superior to the effect of intravenous glucocorticoid, and the adverse effect has decreased.
2012, 36(11): 1315-1317.
Abstract:
Objective: To explore the risk factors and prevention measures of ureteroscopy complications for improving the safety of ureterorenoscope. Methods: The clinical data of 118 cases with ureteroscope detecting and pneumatic lithotripsy through rigid ureteroscope were analysed retrospectively. The preoperative and postoperative complications, surgeon' s clinical experience, operative techniques and skills and the condition of ureter and anesthesia were studied. Results: The complications included ureteral injury(8 cases, 6. 7%) , stone upper migration(12 cases, 11. 7%) , haematuria(10 cases, 8. 5%) , lumbago(9 cases, 7. 6%) and fever(＞ 39 ℃)(3 cases, 2. 5%) . The X-ray of abdominal urinary tract was detected 2 months after operation, and the rates of stone clearance were 98. 1%(101 cases) . Conclusions: The surgeons' skills, their awareness for the risk factors of ureterorenoscope and correct operation methods can effectively prevent the occurrence of clinical complications.
Objective: To explore the risk factors and prevention measures of ureteroscopy complications for improving the safety of ureterorenoscope. Methods: The clinical data of 118 cases with ureteroscope detecting and pneumatic lithotripsy through rigid ureteroscope were analysed retrospectively. The preoperative and postoperative complications, surgeon' s clinical experience, operative techniques and skills and the condition of ureter and anesthesia were studied. Results: The complications included ureteral injury(8 cases, 6. 7%) , stone upper migration(12 cases, 11. 7%) , haematuria(10 cases, 8. 5%) , lumbago(9 cases, 7. 6%) and fever(＞ 39 ℃)(3 cases, 2. 5%) . The X-ray of abdominal urinary tract was detected 2 months after operation, and the rates of stone clearance were 98. 1%(101 cases) . Conclusions: The surgeons' skills, their awareness for the risk factors of ureterorenoscope and correct operation methods can effectively prevent the occurrence of clinical complications.
2012, 36(11): 1330-1331,1335.
Abstract:
Objective: To establish an HP LC method for determination of naftopidil preparations. Methods: In this study, Waters C18 column(150. 0 mm 4. 6 mm, 5 m) was used, with acetonitrile-methanol-0. 02 mol/L sodium acetate(40∶ 30∶ 30) as mobile phase at a flow rate of 1. 0 ml/min, and the detection wavelength was 283 nm. The injection volume was 10 l and column temperature was set at 30 ℃. Results: The calibration curve was over the range of 0. 998-29. 940 g/ml(with the correlation coefficient of 0. 9999) . The mean recovery of three concentration were 101. 61%, 100. 44% and 99. 28% while the RSD(%) was 0. 25, 0. 12 and 0. 44, respectively. Conclusions: The method is simple, accurate, reproducible and suitable for the quality control of Naftopidil preparations.
Objective: To establish an HP LC method for determination of naftopidil preparations. Methods: In this study, Waters C18 column(150. 0 mm 4. 6 mm, 5 m) was used, with acetonitrile-methanol-0. 02 mol/L sodium acetate(40∶ 30∶ 30) as mobile phase at a flow rate of 1. 0 ml/min, and the detection wavelength was 283 nm. The injection volume was 10 l and column temperature was set at 30 ℃. Results: The calibration curve was over the range of 0. 998-29. 940 g/ml(with the correlation coefficient of 0. 9999) . The mean recovery of three concentration were 101. 61%, 100. 44% and 99. 28% while the RSD(%) was 0. 25, 0. 12 and 0. 44, respectively. Conclusions: The method is simple, accurate, reproducible and suitable for the quality control of Naftopidil preparations.
2012, 36(11): 1332-1335.
Abstract:
Objective: To evaluate the bioequivalence of lisinopril tablets in healthy volunteers. Methods: A single oral dose of 10 mg test or reference formulations were given to 20 male healthy volunteers in a randomized crossover design. Three ml of blood was drawn out from the ulnar vein of the volunteers at 1, 2 , 3 , 4 , 6 , 8 , 10, 12, 16, 24, 36, 48 and 60 h before and after the administration. The plasma concentration of lisinopril was determined by LC-MS/MS method. The pharmacokinetic parameters and relative bioavailability were calculated with DAS program to evaluate the bioequivalence of the two formulations. Results: The pharmacokinetic parameters of lisinopril test formulation were obtained as follows:tmax was(6. 1 1. 0) h, Cmax(64. 24 25. 2) g/L, AUC0-60(942. 0 330) g L-1h-1 and AUC0-(969. 6 327) gL-1h-1 ; for reference formulation:tmax was(6. 1 1. 2) h, Cmax(68. 19 26. 5) g/L, AUC0-144(980. 1 340) gL-1h-1 and AUC0-(1002. 3 337) gL-1h-1 . Calculated with AUC0-144 , the bioavailability of the two formulations was(98. 8 27. 5) %. Conclusions: The lisinopril tablet test formulation and reference formulation are bioequivalent.
Objective: To evaluate the bioequivalence of lisinopril tablets in healthy volunteers. Methods: A single oral dose of 10 mg test or reference formulations were given to 20 male healthy volunteers in a randomized crossover design. Three ml of blood was drawn out from the ulnar vein of the volunteers at 1, 2 , 3 , 4 , 6 , 8 , 10, 12, 16, 24, 36, 48 and 60 h before and after the administration. The plasma concentration of lisinopril was determined by LC-MS/MS method. The pharmacokinetic parameters and relative bioavailability were calculated with DAS program to evaluate the bioequivalence of the two formulations. Results: The pharmacokinetic parameters of lisinopril test formulation were obtained as follows:tmax was(6. 1 1. 0) h, Cmax(64. 24 25. 2) g/L, AUC0-60(942. 0 330) g L-1h-1 and AUC0-(969. 6 327) gL-1h-1 ; for reference formulation:tmax was(6. 1 1. 2) h, Cmax(68. 19 26. 5) g/L, AUC0-144(980. 1 340) gL-1h-1 and AUC0-(1002. 3 337) gL-1h-1 . Calculated with AUC0-144 , the bioavailability of the two formulations was(98. 8 27. 5) %. Conclusions: The lisinopril tablet test formulation and reference formulation are bioequivalent.
2012, 36(11): 1336-1337,1340.
Abstract:
Objective: To survey the status of instruments and staffs of clinical laboratories in secondary level country hospitals of Anhui province and provide reference for improving instruments configuration and clinical laboratory examination level. Methods: The secondary level country hospitals in west, middle and south of Anhui province were selected randomly, and sample investigation of equipments and staffs were carried out through questionnaires or inspection methods. Results: There was no regional differences in instruments and equipments in secondary level country hospitals(P ＞ 0. 05) . Among those critical issues, blood gas analyzer was equipped with the lowest rate, PCR instrument was equipped generally with low rate. The rate of instruments maintenance from manufacturers was low generally, and the autonomous maintenance was variable in those country hospitals. The degree of clinical laboratory physician was mainly junior college, and the professional title was primary mostly. The laboratory quality control was received attention widespreadly. Conclusions: In our province, the efficiency of instruments configuration was low for secondary level country hospitals, the instruments configuration and staff training should be improved.
Objective: To survey the status of instruments and staffs of clinical laboratories in secondary level country hospitals of Anhui province and provide reference for improving instruments configuration and clinical laboratory examination level. Methods: The secondary level country hospitals in west, middle and south of Anhui province were selected randomly, and sample investigation of equipments and staffs were carried out through questionnaires or inspection methods. Results: There was no regional differences in instruments and equipments in secondary level country hospitals(P ＞ 0. 05) . Among those critical issues, blood gas analyzer was equipped with the lowest rate, PCR instrument was equipped generally with low rate. The rate of instruments maintenance from manufacturers was low generally, and the autonomous maintenance was variable in those country hospitals. The degree of clinical laboratory physician was mainly junior college, and the professional title was primary mostly. The laboratory quality control was received attention widespreadly. Conclusions: In our province, the efficiency of instruments configuration was low for secondary level country hospitals, the instruments configuration and staff training should be improved.
2012, 36(11): 1338-1340.
Abstract:
Objective: To evaluate the clinical value of measuring serum ischemia modified albumin(IMA) using photocolorimetry. Methods: The IMA levels of 46 cases with cTnI positive or electrocardiogram ST session abnormal and 70 healthy subjects were measured through photocolorimetry, analysed by ROC curve, evaluated by the characters of clinical diagnosis and compared with the ultrafiltration albumin cobalt binding test. Results: The intra and inter-assay CV of the precision test results were 1. 3% and 1. 8%, respectively, and the AUC of ROC curve was 0. 995. When the cutoff value was 75 u/ml, the sensitivity, specificity and accuracy of IMA were 97. 8%, 91. 4% and 94. 0%, respectively, and the positive and negative predictive value were 88. 2% and 98. 5%, respectively. The IMA levels detected by two methods had no statistically significance(P ＞ 0. 05) . Conclusions: Compared with the ultrafiltration albumin cobalt binding test, the photocolorimetry to measure the serum IMA is good repeatability and simple and convenient. The high sensitivity and negative predictive value show the high clinical value of IMA for acute coronary syndromes; exclusion diagnosis or thoracalgia diagnosis.
Objective: To evaluate the clinical value of measuring serum ischemia modified albumin(IMA) using photocolorimetry. Methods: The IMA levels of 46 cases with cTnI positive or electrocardiogram ST session abnormal and 70 healthy subjects were measured through photocolorimetry, analysed by ROC curve, evaluated by the characters of clinical diagnosis and compared with the ultrafiltration albumin cobalt binding test. Results: The intra and inter-assay CV of the precision test results were 1. 3% and 1. 8%, respectively, and the AUC of ROC curve was 0. 995. When the cutoff value was 75 u/ml, the sensitivity, specificity and accuracy of IMA were 97. 8%, 91. 4% and 94. 0%, respectively, and the positive and negative predictive value were 88. 2% and 98. 5%, respectively. The IMA levels detected by two methods had no statistically significance(P ＞ 0. 05) . Conclusions: Compared with the ultrafiltration albumin cobalt binding test, the photocolorimetry to measure the serum IMA is good repeatability and simple and convenient. The high sensitivity and negative predictive value show the high clinical value of IMA for acute coronary syndromes; exclusion diagnosis or thoracalgia diagnosis.
2012, 36(11): 1341-1342.
Abstract:
Objective: To investigate the infection status of Helicobacter pylori(Hp) among healthy population in Bengbu City. Methods: Immune colloidal gold assay was used to detect Hp urease antibody in serum among 2 283 cases of healthy people in Bengbu city. Results: The overall Hp infection rate was 16. 1% in total 2 283 cases. Among them, the infection rate for male was 16. 9%, and for female was 14. 2%, it was no statistical difference among male and female(P ＞ 0. 05) . There were statistical significances about Hp infection rate among different ages in male(P ＜ 0. 01) , but no statistical significance among different ages in female(P ＞ 0. 05) . The infection rates of male in 50-59 age group and above 70 age group were higher than those of female groups and was statistically significant(P ＜ 0. 05) . Conclusions: The infection rate of Hp was high in healthy population in Bengbu. Hence, Hp detection should be routinely conducted in physical examination. The early precaution will be useful for early cure and prevent relevant diseases.
Objective: To investigate the infection status of Helicobacter pylori(Hp) among healthy population in Bengbu City. Methods: Immune colloidal gold assay was used to detect Hp urease antibody in serum among 2 283 cases of healthy people in Bengbu city. Results: The overall Hp infection rate was 16. 1% in total 2 283 cases. Among them, the infection rate for male was 16. 9%, and for female was 14. 2%, it was no statistical difference among male and female(P ＞ 0. 05) . There were statistical significances about Hp infection rate among different ages in male(P ＜ 0. 01) , but no statistical significance among different ages in female(P ＞ 0. 05) . The infection rates of male in 50-59 age group and above 70 age group were higher than those of female groups and was statistically significant(P ＜ 0. 05) . Conclusions: The infection rate of Hp was high in healthy population in Bengbu. Hence, Hp detection should be routinely conducted in physical examination. The early precaution will be useful for early cure and prevent relevant diseases.
2012, 36(11): 1343-1344.
Abstract:
Objective: To investigate the relationship between sperm vigor and sperm morphology parameters. Methods: The sperm specimens of 6 725 cases with male infertility were stained by Diff-Quik to analyse their morphologies. Results: The percentage of normal sperms in morphology with normal vigor was significantly higher than that of the sperms with abnormal vigor(P ＜ 0. 01) . The percentage of head, midpiece and tail faults and the index of deformity sperm of sperms with normal vigor were significantly lower than those of the sperms with abnormal vigor(P ＜ 0. 01) . Conclusions: The percentage of normal morphology and head, midpiece and tail faults and the index of deformity sperm of sperms are strongly linked to their vigor, which can be used as an important parameter of male fertility.
Objective: To investigate the relationship between sperm vigor and sperm morphology parameters. Methods: The sperm specimens of 6 725 cases with male infertility were stained by Diff-Quik to analyse their morphologies. Results: The percentage of normal sperms in morphology with normal vigor was significantly higher than that of the sperms with abnormal vigor(P ＜ 0. 01) . The percentage of head, midpiece and tail faults and the index of deformity sperm of sperms with normal vigor were significantly lower than those of the sperms with abnormal vigor(P ＜ 0. 01) . Conclusions: The percentage of normal morphology and head, midpiece and tail faults and the index of deformity sperm of sperms are strongly linked to their vigor, which can be used as an important parameter of male fertility.
2012, 36(11): 1345-1346.
Abstract:
Objective: To explore the application of color Doppler ultrasound in the acute scrotum. Methods: The results of color Doppler ultrasound examination of 72 patients with acute scrotum were retrospectively analyzed, and compared with following-up results. Results: Of 23 cases with testicular torsion, 22 cases were diagnosed by ultrasonography, which ultrasonography displayed mainly raising testicular location, transverse or oblique, abnormal morphology, uneven hypoechoic and blood flow signal loss. Of 45 cases with epididymitis, 44 cases were diagnosed by ultrasonography, which ultrasonography displayed mainly the increasing testis and epididymis, reducting and uneven local echo. Four cases with testicular trauma were correctly diagnosed by ultrasonography, which ultrasonography displayed mainly no complete testicular morphology, partial envelope echo interrupt and uneven echo in injury area. Conclusions: Color Doppler ultrasound can reliably diagnose and differentially diagose the acute scrotum, which can provide the important application value for clinical diagnosis and treatment.
Objective: To explore the application of color Doppler ultrasound in the acute scrotum. Methods: The results of color Doppler ultrasound examination of 72 patients with acute scrotum were retrospectively analyzed, and compared with following-up results. Results: Of 23 cases with testicular torsion, 22 cases were diagnosed by ultrasonography, which ultrasonography displayed mainly raising testicular location, transverse or oblique, abnormal morphology, uneven hypoechoic and blood flow signal loss. Of 45 cases with epididymitis, 44 cases were diagnosed by ultrasonography, which ultrasonography displayed mainly the increasing testis and epididymis, reducting and uneven local echo. Four cases with testicular trauma were correctly diagnosed by ultrasonography, which ultrasonography displayed mainly no complete testicular morphology, partial envelope echo interrupt and uneven echo in injury area. Conclusions: Color Doppler ultrasound can reliably diagnose and differentially diagose the acute scrotum, which can provide the important application value for clinical diagnosis and treatment.
2012, 36(11): 1357-1361.
Abstract:
Objective: To summarize the common problems of the results content expression in the original medical manuscript in order to provide the authors' writting with draw lesson. Methods: The common problems of the results content were analyzed by some examples, editing measures were proposed appropriately, and the correct forms of the expression were elaborated. Results: There were most common problems such as literature instead of results, non-accurate or non-specific figure expression, the repetitions between script and graph, script and table or graph and table, confusions of results and discussion or methods and results, un-detailed location of graph or table, etc. Conclusions: The authors must grasp the expression forms of the result contents and handle the relationship among the expressions of charts, tables and script of medical thesis correctly.
Objective: To summarize the common problems of the results content expression in the original medical manuscript in order to provide the authors' writting with draw lesson. Methods: The common problems of the results content were analyzed by some examples, editing measures were proposed appropriately, and the correct forms of the expression were elaborated. Results: There were most common problems such as literature instead of results, non-accurate or non-specific figure expression, the repetitions between script and graph, script and table or graph and table, confusions of results and discussion or methods and results, un-detailed location of graph or table, etc. Conclusions: The authors must grasp the expression forms of the result contents and handle the relationship among the expressions of charts, tables and script of medical thesis correctly.
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