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2012 Vol. 37, No. 10
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2012, 36(10): 1141-1144.
Abstract:
Objective: To detect the changes of the number of NK cells and intracellular killing molecules in peripheral blood of patients with pulmonary tuberculosis. Methods: Percentages of NK cells in peripheral whole blood samples from 145 pulmonary TB patients(105 first-treated pulmonary TB and 40 retreated pulmonary TB) and 112 healthy controls were collected, labelled with anti- CD3,CD56,CD8, granulysin,perforin and granzyme antibody, and analyzed by flow cytometry. Results: The percentage of NK cells in first-treated pulmonary TB patients was(16. 95 8. 27) %,which was significantly lower than that in healthy controls(19. 99 9. 28) % (P ＜ 0. 01 ) . Absolute value of NK cells in first-treated pulmonary TB patients was (33. 31 21. 44 ) 104 /ml,which was significantly lower than that in healthy controls (44. 32 26. 69) 104 /ml) (P ＜ 0. 05) . Absolute value of NK cells in retreated pulmonary TB patients was (36. 8 24. 97) 104 /ml. Granulysin［(85. 78 11. 63) % vs (87. 31 10. 34) %］,perforin［(82. 88 17. 05) % vs (82. 85 13. 03) %］in NK cells had no difference bewteen pulmonary TB patients and healthy controls(P ＞ 0. 05) ,but the difference of granzyme［(79. 07 15. 64) % vs (88. 04 7. 27) %］was significant(P ＜ 0. 01) . Conclusions: The number of NK cells in peripheral whole blood of first-treated pulmonary TB patients NK was significantly decreased. However, the expressions of granulysin and perforin in NK cells had no difference between pulmonary TB patients and healthy controls.
Objective: To detect the changes of the number of NK cells and intracellular killing molecules in peripheral blood of patients with pulmonary tuberculosis. Methods: Percentages of NK cells in peripheral whole blood samples from 145 pulmonary TB patients(105 first-treated pulmonary TB and 40 retreated pulmonary TB) and 112 healthy controls were collected, labelled with anti- CD3,CD56,CD8, granulysin,perforin and granzyme antibody, and analyzed by flow cytometry. Results: The percentage of NK cells in first-treated pulmonary TB patients was(16. 95 8. 27) %,which was significantly lower than that in healthy controls(19. 99 9. 28) % (P ＜ 0. 01 ) . Absolute value of NK cells in first-treated pulmonary TB patients was (33. 31 21. 44 ) 104 /ml,which was significantly lower than that in healthy controls (44. 32 26. 69) 104 /ml) (P ＜ 0. 05) . Absolute value of NK cells in retreated pulmonary TB patients was (36. 8 24. 97) 104 /ml. Granulysin［(85. 78 11. 63) % vs (87. 31 10. 34) %］,perforin［(82. 88 17. 05) % vs (82. 85 13. 03) %］in NK cells had no difference bewteen pulmonary TB patients and healthy controls(P ＞ 0. 05) ,but the difference of granzyme［(79. 07 15. 64) % vs (88. 04 7. 27) %］was significant(P ＜ 0. 01) . Conclusions: The number of NK cells in peripheral whole blood of first-treated pulmonary TB patients NK was significantly decreased. However, the expressions of granulysin and perforin in NK cells had no difference between pulmonary TB patients and healthy controls.
2012, 36(10): 1144-1146.
Abstract:
Objective: To analyze the changes of dendritic cells (DC) and its subsets in peripheral blood of patients with vivax malaria, and explore its immune mechanism. Methods: Eighteen patients with vivax malaria (malaria group) and 36 healthy adults (control group) were selected. The proportions of plasmacytoid DC(pDC) and myeloid DC(mDC) in peripheral blood were detected by flow cytometry four-colour fluorimetric analysis technique. Results: pDC subset in malaria group was significantly lower than that in control group(P ＜ 0. 01) . mDC/pDC in malaria group was significantly higher than that in control group(P ＜ 0. 01) , and there was no difference in the proportion of mDC between the two groups(P ＞ 0. 05) . Conclusions: There presents a general immunosuppression during plasmodium vivax infections. Plasmodium vivax parasites interact with DC,which involves inducing and adjusting Th1 immune response by altering mDC/pDC ratio.
Objective: To analyze the changes of dendritic cells (DC) and its subsets in peripheral blood of patients with vivax malaria, and explore its immune mechanism. Methods: Eighteen patients with vivax malaria (malaria group) and 36 healthy adults (control group) were selected. The proportions of plasmacytoid DC(pDC) and myeloid DC(mDC) in peripheral blood were detected by flow cytometry four-colour fluorimetric analysis technique. Results: pDC subset in malaria group was significantly lower than that in control group(P ＜ 0. 01) . mDC/pDC in malaria group was significantly higher than that in control group(P ＜ 0. 01) , and there was no difference in the proportion of mDC between the two groups(P ＞ 0. 05) . Conclusions: There presents a general immunosuppression during plasmodium vivax infections. Plasmodium vivax parasites interact with DC,which involves inducing and adjusting Th1 immune response by altering mDC/pDC ratio.
2012, 36(10): 1147-1150,1161.
Abstract:
Objective: To isolate and culture of umbilical cord blood mesenchymal stem cells(UCB MSCs) from human umbilical cord blood and explore the effect of induction indoleamine2,3-dioxygenase(IDO) expresson on the treatment of immune mediated aplastic anemia(AA) in mice. Methods: UCB was collected from full-term deliveries. UCB MSCs were isolated and cultured using the lymphocyte isolation method. The cell morphologhy was observed under inverted microscope,and the surface markers of MSCs were analyzed by flow cytometry. Osteogenic and adipogenic induction was used to verify the differentiation potential of UCB MSCs. The murine model with immune mediated aplastic anemia were established. The caudal vein of mice was injected with UCB MSCs induced by IFN-, and the treatment effect was observed. Results: A little of adherent spindle cells presented at 1 week culture of UCB MSCs. About 3 weeks of culture, these uniformity fibroblast-like cells were found, and showed parallelly or whirlpool-shape arrangement. After passage,MSCs reached 80% confluence at 1 week. Immunophenotype analysis showed that MSCs expressed CD44(94. 36%) and CD73 (92. 48%) ,but no CD34 of hematopoietic marker. UCB MSCs that induced by IFN- promoted IDO activity, and had therapeutic effect on haematogenesis function recovery of mice with aplastic anemia. Conclusions: Under IFN- induction, the isolated and cultured UCB MSCs from umbilical cord blood can improve the IDO activity,which has therapeutic effect on murine immune mediated aplastic anemia.
Objective: To isolate and culture of umbilical cord blood mesenchymal stem cells(UCB MSCs) from human umbilical cord blood and explore the effect of induction indoleamine2,3-dioxygenase(IDO) expresson on the treatment of immune mediated aplastic anemia(AA) in mice. Methods: UCB was collected from full-term deliveries. UCB MSCs were isolated and cultured using the lymphocyte isolation method. The cell morphologhy was observed under inverted microscope,and the surface markers of MSCs were analyzed by flow cytometry. Osteogenic and adipogenic induction was used to verify the differentiation potential of UCB MSCs. The murine model with immune mediated aplastic anemia were established. The caudal vein of mice was injected with UCB MSCs induced by IFN-, and the treatment effect was observed. Results: A little of adherent spindle cells presented at 1 week culture of UCB MSCs. About 3 weeks of culture, these uniformity fibroblast-like cells were found, and showed parallelly or whirlpool-shape arrangement. After passage,MSCs reached 80% confluence at 1 week. Immunophenotype analysis showed that MSCs expressed CD44(94. 36%) and CD73 (92. 48%) ,but no CD34 of hematopoietic marker. UCB MSCs that induced by IFN- promoted IDO activity, and had therapeutic effect on haematogenesis function recovery of mice with aplastic anemia. Conclusions: Under IFN- induction, the isolated and cultured UCB MSCs from umbilical cord blood can improve the IDO activity,which has therapeutic effect on murine immune mediated aplastic anemia.
2012, 36(10): 1151-1154,1158.
Abstract:
Objective: To study whether curcumin has the protective effect on atherosclerosis via modulation inflammation and endothelial function. Methods: Human umbilical veins endodermis cells(HUVEC) were cultured in vitro. The effects of curcumin on the changes of vascular endothelial cell morphology and the expressions of inflammation factors including inter-cellular adhesion molecule-1(ICAM-1) , interleukin-6 (IL-6) and interleukin-8(IL-8) were studied on the early phase model of atherosclerosis induced by oxidative low-density lipoprotein(OX-LDL) . Results: Compared with control group,OX-LDL inhibited the proliferation of HUVEC and induced the expressions of ICAM-1, IL-6 and IL-8 in HUVEC,which were higher than in control group(P ＜ 0. 01) . Compared with ox-LDL group,curcumin reduced the apoptosis of HUVEC, the expressions of ICAM-1, IL-6 and IL-8 were decreased,which were statistical different(P ＜ 0. 01) . Conclusions: Curcumin can improve atherosclerosis and protect vascular endothelial cells by inhibition of the expressions of inflammatory factors.
Objective: To study whether curcumin has the protective effect on atherosclerosis via modulation inflammation and endothelial function. Methods: Human umbilical veins endodermis cells(HUVEC) were cultured in vitro. The effects of curcumin on the changes of vascular endothelial cell morphology and the expressions of inflammation factors including inter-cellular adhesion molecule-1(ICAM-1) , interleukin-6 (IL-6) and interleukin-8(IL-8) were studied on the early phase model of atherosclerosis induced by oxidative low-density lipoprotein(OX-LDL) . Results: Compared with control group,OX-LDL inhibited the proliferation of HUVEC and induced the expressions of ICAM-1, IL-6 and IL-8 in HUVEC,which were higher than in control group(P ＜ 0. 01) . Compared with ox-LDL group,curcumin reduced the apoptosis of HUVEC, the expressions of ICAM-1, IL-6 and IL-8 were decreased,which were statistical different(P ＜ 0. 01) . Conclusions: Curcumin can improve atherosclerosis and protect vascular endothelial cells by inhibition of the expressions of inflammatory factors.
2012, 36(10): 1159-1161.
Abstract:
Objective: To explore the significance of cervical acid phosphatase (CAP) in the early diagnosis of cervical cancer. Methods: The CAP expression of cervical smears of 350 cases was detected by immunohistochemical staining method with Mark-PAP kit. The red granular deposit in cytoplasm was regarded as CAP positive,which was compared with the Results diagnosed by Thinprep cytologic test(TCT) . Results: The CAP expression of the cervical smears of the patents with cervical cancer was confirmed,while CAP was negative in normal and inflammatory cervical cells. The Results of the CAP test and TCT test were consistent. Conclusions: The CAP expression may be instructive significance in the early diagnosis of cervical cancer.
Objective: To explore the significance of cervical acid phosphatase (CAP) in the early diagnosis of cervical cancer. Methods: The CAP expression of cervical smears of 350 cases was detected by immunohistochemical staining method with Mark-PAP kit. The red granular deposit in cytoplasm was regarded as CAP positive,which was compared with the Results diagnosed by Thinprep cytologic test(TCT) . Results: The CAP expression of the cervical smears of the patents with cervical cancer was confirmed,while CAP was negative in normal and inflammatory cervical cells. The Results of the CAP test and TCT test were consistent. Conclusions: The CAP expression may be instructive significance in the early diagnosis of cervical cancer.
2012, 36(10): 1162-1165.
Abstract:
Objective: To investgate the expression and clinical significance of the deathreceptor 5(DR 5) and decoyreceptor 2(DcR 2) of TNF-related apoptosis-inducing ligand (TRAIL) in endometrial cancer. Methods: The expressions of DR5 and DcR2 of endometrium tissues from 52 cases with endometrial cancer,20 cases with atypical endometrium hyperplasia and 23 cases normal endometrium tissue were detected by immunohistochemistry. The pathological grade of surgery, tumor differentiation degree, tissue type, muscular layer infiltration and clinical pathological factors of endometrial cancer were analyzed. Results: The expressions of DcR2 of normal endometrium tissue were higher than that of endometrial cancer tissue (P ＜ 0. 01 ) . The expressions of DR5 of normal endometrium,atypical endometrium hyperplasia and endometrial cancer tissue showed increasing trend,the Results of multiple comparison had statistically significant (P ＜ 0. 01) . Conclusions: Endometrium carcinoma cells can be easily induced apoptosis by TRAIL with high expression of DR5 and low expression of DcR2,which can provide a basis for treating endometrium carcinoma.
Objective: To investgate the expression and clinical significance of the deathreceptor 5(DR 5) and decoyreceptor 2(DcR 2) of TNF-related apoptosis-inducing ligand (TRAIL) in endometrial cancer. Methods: The expressions of DR5 and DcR2 of endometrium tissues from 52 cases with endometrial cancer,20 cases with atypical endometrium hyperplasia and 23 cases normal endometrium tissue were detected by immunohistochemistry. The pathological grade of surgery, tumor differentiation degree, tissue type, muscular layer infiltration and clinical pathological factors of endometrial cancer were analyzed. Results: The expressions of DcR2 of normal endometrium tissue were higher than that of endometrial cancer tissue (P ＜ 0. 01 ) . The expressions of DR5 of normal endometrium,atypical endometrium hyperplasia and endometrial cancer tissue showed increasing trend,the Results of multiple comparison had statistically significant (P ＜ 0. 01) . Conclusions: Endometrium carcinoma cells can be easily induced apoptosis by TRAIL with high expression of DR5 and low expression of DcR2,which can provide a basis for treating endometrium carcinoma.
2012, 36(10): 1166-1169.
Abstract:
Objective: To explore the characteristics and related factors of coronary artery lesions in patients with premature coronary heart disease. Methods: One hundred and two patients diagnosed coronary heart disease by coronary angiography were divided into early onset group(43 cases,male ＜ 55 years old and female ＜ 65 years old) and late onset group(59 cases,male 55 years old and female 65 years old) . The characteristics and related factors of coronary artery lesions of two groups were analysed. Results: The proportion of single vascular lesions in patients with premature coronary heart disease were significantly higher than that of late onset coronary heart disease group(P ＜ 0. 01) . The smoking rate, family history of ischemic cardiovascular disease,body mass index,blood triglycerides, hemoglobin and blood fibrinogen levels of early onset group were obviously higher than that of late onset group(P ＜ 0. 05 to P ＜ 0. 01) . The uric acid levels of early onset group were lower than that of late onset group(P ＜ 0. 05) . Conclusions: The incidence of premature coronary artery disease is closely related to the family history of ischemic cardiovascular disease, fat,high blood triglycerides and the blood fibrinogen level. The proportion of single vascular lesions is more than that of late onset group. To prevent the coronary heart disease should actively control the related risk factors of cardiovascular disease as soon as possible.
Objective: To explore the characteristics and related factors of coronary artery lesions in patients with premature coronary heart disease. Methods: One hundred and two patients diagnosed coronary heart disease by coronary angiography were divided into early onset group(43 cases,male ＜ 55 years old and female ＜ 65 years old) and late onset group(59 cases,male 55 years old and female 65 years old) . The characteristics and related factors of coronary artery lesions of two groups were analysed. Results: The proportion of single vascular lesions in patients with premature coronary heart disease were significantly higher than that of late onset coronary heart disease group(P ＜ 0. 01) . The smoking rate, family history of ischemic cardiovascular disease,body mass index,blood triglycerides, hemoglobin and blood fibrinogen levels of early onset group were obviously higher than that of late onset group(P ＜ 0. 05 to P ＜ 0. 01) . The uric acid levels of early onset group were lower than that of late onset group(P ＜ 0. 05) . Conclusions: The incidence of premature coronary artery disease is closely related to the family history of ischemic cardiovascular disease, fat,high blood triglycerides and the blood fibrinogen level. The proportion of single vascular lesions is more than that of late onset group. To prevent the coronary heart disease should actively control the related risk factors of cardiovascular disease as soon as possible.
2012, 36(10): 1169-1170.
Abstract:
Objective: To explore the effects of sodium aescinate on preventing cluster headache. Methods: Twenty-two patients with episodic cluster headache were treated with sodium aescinate(20 mg /d) by intravenous infusion for 10 days, and then using tablet by oral(0. 3 g /d, twice a day) . The frequency and duration of headache of all patients were observed,which was compared with that of pretreatment. Results: The duration and frequency of cluster headache of 22 patients after treatment were obviously shorter and lower than that of pre-treatment(P ＜ 0. 05) . Conclusions: The effects of sodium aescinate treating cluster headache are good. Sodium aescinate is little side-effect and well tolerated,which is an ideal drug to treat episodic cluster headache.
Objective: To explore the effects of sodium aescinate on preventing cluster headache. Methods: Twenty-two patients with episodic cluster headache were treated with sodium aescinate(20 mg /d) by intravenous infusion for 10 days, and then using tablet by oral(0. 3 g /d, twice a day) . The frequency and duration of headache of all patients were observed,which was compared with that of pretreatment. Results: The duration and frequency of cluster headache of 22 patients after treatment were obviously shorter and lower than that of pre-treatment(P ＜ 0. 05) . Conclusions: The effects of sodium aescinate treating cluster headache are good. Sodium aescinate is little side-effect and well tolerated,which is an ideal drug to treat episodic cluster headache.
2012, 36(10): 1171-1173,1177.
Abstract:
Objective: To evaluate the long-term effects of vascular pedicled iliac periosteal flap graft on Perthes disease. Methods: Fifty-three hips of 49 patients with Perthes disease were treated with vascular pedicled iliac periosteal flap. All cases were followed-up for 3 to 16 years. According to the lateral pillar classification, 19 hips were B type, 23 hips as B/C type and 11 hips as C type. Results: The Harris hip scores were obviously improved after treatment(P ＜ 0. 01) . According to the Stulberg classification system modified, 39 hips were StulbergclassⅠor Ⅱ grade including 16 hips as Herring B type, 19 hips as B/C type and 4 hips as C type. Ten hips were Stulbergclass Ⅲ grade including 3 hips as Herring B type,3 hips as B/C type and 4 hips as C type. Four hips were Stulbergclass Ⅳ grade including 1 hip as B/C type and 3 hips as C type. There was not statistical difference between the Stulberg grades of B and B/C type postoperation(P ＞ 0. 05) . Compared with C type, the Stulberg grades of B and B/C type had significantly different(P ＜ 0. 01) . The prognosis of 34 hips in male(41 hips) and 5 hips in female(12 hips) were StulbergclassⅠor Ⅱgrade. The prognosis of 32 hips in 4 to 7 years children(37 hips) and 7 hips in 8 to 12 years children(16 hips) were StulbergclassⅠor Ⅱgrade. Conclusions: The effects of treating Perthes disease with vascular pedicled iliac periosteal flap graft are good. The lateral pillar classification, the age and gender of patients can help us to evaluate prognosis.
Objective: To evaluate the long-term effects of vascular pedicled iliac periosteal flap graft on Perthes disease. Methods: Fifty-three hips of 49 patients with Perthes disease were treated with vascular pedicled iliac periosteal flap. All cases were followed-up for 3 to 16 years. According to the lateral pillar classification, 19 hips were B type, 23 hips as B/C type and 11 hips as C type. Results: The Harris hip scores were obviously improved after treatment(P ＜ 0. 01) . According to the Stulberg classification system modified, 39 hips were StulbergclassⅠor Ⅱ grade including 16 hips as Herring B type, 19 hips as B/C type and 4 hips as C type. Ten hips were Stulbergclass Ⅲ grade including 3 hips as Herring B type,3 hips as B/C type and 4 hips as C type. Four hips were Stulbergclass Ⅳ grade including 1 hip as B/C type and 3 hips as C type. There was not statistical difference between the Stulberg grades of B and B/C type postoperation(P ＞ 0. 05) . Compared with C type, the Stulberg grades of B and B/C type had significantly different(P ＜ 0. 01) . The prognosis of 34 hips in male(41 hips) and 5 hips in female(12 hips) were StulbergclassⅠor Ⅱgrade. The prognosis of 32 hips in 4 to 7 years children(37 hips) and 7 hips in 8 to 12 years children(16 hips) were StulbergclassⅠor Ⅱgrade. Conclusions: The effects of treating Perthes disease with vascular pedicled iliac periosteal flap graft are good. The lateral pillar classification, the age and gender of patients can help us to evaluate prognosis.
2012, 36(10): 1174-1177.
Abstract:
Objective: To investigate the oxidative stress condition in patients with different impaired glucose regulation and analyze the relevance of oxidative stress and insulin resistance(IR) and -cell function. Methods: Twenty-eight patients with normal glucose tolerance(NGT) , 30 with impaired fasting glucose(IFG) , 30 with impaired glucose tolerance(IGT) and 32 with type 2 diabetes mellitus (T2DM) were observed. The serum SOD,GSH-PX and MDA levels in all cases were measured,with HOMA-IR,HOMA-,I30 / G30,MBCI respectively to assess IR, the basal insulin secretion, early stage of insulin secretion and insulin secretion after glucose load. Results: Compared to NGT, the serum MDA level was increased from NGT、IFG、IGT toT2DM(P ＜ 0. 01) . Compared to IFG, there was statistical difference in IGT and T2DM(P ＜ 0. 01) . There was no statistical difference between IGT and T2DM(P ＞ 0. 05) . The serum SOD,GSH-PX levels were decreased from NGT, IFG, IGT to T2DM. There was statistical difference between NGT and T2DM and no statistical difference among IFG,IGT and T2DM. There was positive correlation between MDA and HOMA-IR,between SOD and HOMA-,△I30 /△G30,MBCI,between GSH-PX and HOMA-,MBCI. There was negative correlation between MDA and HOMA-, △I30 /△G30,MBCI,between SOD and HOMA-IR. Conclusions: IR and -cell function decline was important pathophysiological changes in type 2 diabetes mellitus and emerged before the diagnosis of diabetes. From NGT、IFG、IGT to T2DM, the degree of oxidative stress was getting higher and oxidative injury was existed in pre-diabetes mellitus. There was confidential correlation between oxidative stress and IR and -cell function. Oxidative stress may have an important function with the development of T2DM.
Objective: To investigate the oxidative stress condition in patients with different impaired glucose regulation and analyze the relevance of oxidative stress and insulin resistance(IR) and -cell function. Methods: Twenty-eight patients with normal glucose tolerance(NGT) , 30 with impaired fasting glucose(IFG) , 30 with impaired glucose tolerance(IGT) and 32 with type 2 diabetes mellitus (T2DM) were observed. The serum SOD,GSH-PX and MDA levels in all cases were measured,with HOMA-IR,HOMA-,I30 / G30,MBCI respectively to assess IR, the basal insulin secretion, early stage of insulin secretion and insulin secretion after glucose load. Results: Compared to NGT, the serum MDA level was increased from NGT、IFG、IGT toT2DM(P ＜ 0. 01) . Compared to IFG, there was statistical difference in IGT and T2DM(P ＜ 0. 01) . There was no statistical difference between IGT and T2DM(P ＞ 0. 05) . The serum SOD,GSH-PX levels were decreased from NGT, IFG, IGT to T2DM. There was statistical difference between NGT and T2DM and no statistical difference among IFG,IGT and T2DM. There was positive correlation between MDA and HOMA-IR,between SOD and HOMA-,△I30 /△G30,MBCI,between GSH-PX and HOMA-,MBCI. There was negative correlation between MDA and HOMA-, △I30 /△G30,MBCI,between SOD and HOMA-IR. Conclusions: IR and -cell function decline was important pathophysiological changes in type 2 diabetes mellitus and emerged before the diagnosis of diabetes. From NGT、IFG、IGT to T2DM, the degree of oxidative stress was getting higher and oxidative injury was existed in pre-diabetes mellitus. There was confidential correlation between oxidative stress and IR and -cell function. Oxidative stress may have an important function with the development of T2DM.
2012, 36(10): 1178-1180.
Abstract:
Objective: To investigate the dynamic changes of vascular endothelial growth factor(VEGF) and angiopoietin-1(Ang-1) levels in serum and its significance of patients with acute cerebral infarction and the relationship between the content of two kinds of growth factor and infarction area, and theirselves. Methods: The serum levels of VEGF and Ang-1 of 30 controls and 50 patients with acute cerebral infarction at hours 48,days 2,3 ,7 , 14 and 21 were measured by ELISA. Results: The levels of VEGF in patients with acute cerebral infarction at different time-points were significantly higher than that of control group(P ＜ 0. 01) ,which were dynamic changes and peaked on the 3rd day(P ＜ 0. 01) . The levels of VEGF were closely related to infarction size(P ＜ 0. 05 ～ P ＜ 0. 01) . The levels of Ang-1 in patients with acute cerebral infarction increased on the 7th day and peaked on the 14th day,which were related to infarction size and positive correlation with VEGF content(P ＜ 0. 05) . Conclusions: The serum levels of VEGF and Ang-1 in patients with cerebral infarction increase significantly,which suggest that the two angiogenic factros involve in the pathophysiological process of ischemic brain damage. The serum levels of VFGF and Ang-1 have a certain significance to estimate the infarction size.
Objective: To investigate the dynamic changes of vascular endothelial growth factor(VEGF) and angiopoietin-1(Ang-1) levels in serum and its significance of patients with acute cerebral infarction and the relationship between the content of two kinds of growth factor and infarction area, and theirselves. Methods: The serum levels of VEGF and Ang-1 of 30 controls and 50 patients with acute cerebral infarction at hours 48,days 2,3 ,7 , 14 and 21 were measured by ELISA. Results: The levels of VEGF in patients with acute cerebral infarction at different time-points were significantly higher than that of control group(P ＜ 0. 01) ,which were dynamic changes and peaked on the 3rd day(P ＜ 0. 01) . The levels of VEGF were closely related to infarction size(P ＜ 0. 05 ～ P ＜ 0. 01) . The levels of Ang-1 in patients with acute cerebral infarction increased on the 7th day and peaked on the 14th day,which were related to infarction size and positive correlation with VEGF content(P ＜ 0. 05) . Conclusions: The serum levels of VEGF and Ang-1 in patients with cerebral infarction increase significantly,which suggest that the two angiogenic factros involve in the pathophysiological process of ischemic brain damage. The serum levels of VFGF and Ang-1 have a certain significance to estimate the infarction size.
The investigation and analysis of the preoperative nutrition risk of patients with esophageal cancer
2012, 36(10): 1181-1182,1185.
Abstract:
Objective: To screen the preoperative nutrition risks of patients with esophageal cancer (EC) ,and discuss the correlation between the nutrition risk(NR) and clinical features. Methods: The preoperative NR and correlation between NR and clinical features of 80 cases with EC were analyzed using the Nutritional Risk Screening 2002 (NRS 2002) . Malnutrition was determined according to the body mass index of Chinese standard (lowered than 18. 5 kg /m2 ) and clinical data,which was recorded as 3 points. Results: Of the 80 EC patients who were evaluated by NRS 2002,64 patients (80. 0%) were judged at malnutrition risk,among these 64 patients, according to the age of EC patients,65. 6% were 60 years old and 34. 4% were ＜ 60 years old. The incidence of the malnutrition risk in patients with early,middle and late stage were 1. 6%,78. 1% and 20. 3%,respectively,and difference of three stages was statistical significant(P ＜ 0. 05 or P ＜ 0. 01) . The incidence of malnutrition risk was not correlated with the sex,lesion location, pathological type and degree of histology(P ＞ 0. 05) . Conclusions: The incidence of malnutrition risk of patients with EC is high,NRS 2002 can help to find the NR of patients with EC,which has relations with the age and pathological stage of EC.
Objective: To screen the preoperative nutrition risks of patients with esophageal cancer (EC) ,and discuss the correlation between the nutrition risk(NR) and clinical features. Methods: The preoperative NR and correlation between NR and clinical features of 80 cases with EC were analyzed using the Nutritional Risk Screening 2002 (NRS 2002) . Malnutrition was determined according to the body mass index of Chinese standard (lowered than 18. 5 kg /m2 ) and clinical data,which was recorded as 3 points. Results: Of the 80 EC patients who were evaluated by NRS 2002,64 patients (80. 0%) were judged at malnutrition risk,among these 64 patients, according to the age of EC patients,65. 6% were 60 years old and 34. 4% were ＜ 60 years old. The incidence of the malnutrition risk in patients with early,middle and late stage were 1. 6%,78. 1% and 20. 3%,respectively,and difference of three stages was statistical significant(P ＜ 0. 05 or P ＜ 0. 01) . The incidence of malnutrition risk was not correlated with the sex,lesion location, pathological type and degree of histology(P ＞ 0. 05) . Conclusions: The incidence of malnutrition risk of patients with EC is high,NRS 2002 can help to find the NR of patients with EC,which has relations with the age and pathological stage of EC.
The investigation and analysis of the preoperative nutrition risk of patients with esophageal cancer
2012, 36(10): 1183-1185.
Abstract:
Objective: To screen the preoperative nutrition risks of patients with esophageal cancer (EC) ,and discuss the correlation between the nutrition risk(NR) and clinical features. Methods: The preoperative NR and correlation between NR and clinical features of 80 cases with EC were analyzed using the Nutritional Risk Screening 2002 (NRS 2002) . Malnutrition was determined according to the body mass index of Chinese standard (lowered than 18. 5 kg /m2 ) and clinical data,which was recorded as 3 points. Results: Of the 80 EC patients who were evaluated by NRS 2002,64 patients (80. 0%) were judged at malnutrition risk,among these 64 patients, according to the age of EC patients,65. 6% were = 60 years old and 34. 4% were ＜ 60 years old. The incidence of the malnutrition risk in patients with early,middle and late stage were 1. 6%,78. 1% and 20. 3%,respectively,and difference of three stages was statistical significant (P ＜ 0. 05 or P ＜ 0. 01) . The incidence of malnutrition risk was not correlated with the sex,lesion location, pathological type and degree of histology(P ＞ 0. 05) . Conclusions: The incidence of malnutrition risk of patients with EC is high,NRS 2002 can help to find the NR of patients with EC,which has relations with the age and pathological stage of EC.
Objective: To screen the preoperative nutrition risks of patients with esophageal cancer (EC) ,and discuss the correlation between the nutrition risk(NR) and clinical features. Methods: The preoperative NR and correlation between NR and clinical features of 80 cases with EC were analyzed using the Nutritional Risk Screening 2002 (NRS 2002) . Malnutrition was determined according to the body mass index of Chinese standard (lowered than 18. 5 kg /m2 ) and clinical data,which was recorded as 3 points. Results: Of the 80 EC patients who were evaluated by NRS 2002,64 patients (80. 0%) were judged at malnutrition risk,among these 64 patients, according to the age of EC patients,65. 6% were = 60 years old and 34. 4% were ＜ 60 years old. The incidence of the malnutrition risk in patients with early,middle and late stage were 1. 6%,78. 1% and 20. 3%,respectively,and difference of three stages was statistical significant (P ＜ 0. 05 or P ＜ 0. 01) . The incidence of malnutrition risk was not correlated with the sex,lesion location, pathological type and degree of histology(P ＞ 0. 05) . Conclusions: The incidence of malnutrition risk of patients with EC is high,NRS 2002 can help to find the NR of patients with EC,which has relations with the age and pathological stage of EC.
2012, 36(10): 1186-1189.
Abstract:
Objective: To investigate the expression and clinical significance of 4-hydroxynonenal (4-HNE) in prostate cancer. Methods: The 4-HNE expression of 67 specimens of prostate cancer tissue and 78 specimens of prostate hyperplasia tissue were detected by immunohistochemistry,and the relationship between its expression and the Gleason grade was analysed. The content of 4- HNE of prostate cancer and prostate hyperplasia patients and free prostate disease elderly men were detected and compared by ELISA. Results: The expression postive rates of 4-HNE in prostatic cancer and prostatic hyperplasia were 100. 00% and 2. 56% respecively, and their difference had statistical significance(P ＜ 0. 01) . There was no correlation between the expression of 4-HNE and Gleason grade(P ＞ 0. 05) . The contents of 4-HNE in the serums of prostate cancer patients were obviously higher than those of prostate hyperplasia and free prostate disease patients(P ＜ 0. 01) . Conclusions: The expression levels of 4-HNE are high in prostate cancer tissue and serum of patient. 4-HNE may play an important role on the mechanism of prostate cancer happens,which can be used to diagnose prostate cancer.
Objective: To investigate the expression and clinical significance of 4-hydroxynonenal (4-HNE) in prostate cancer. Methods: The 4-HNE expression of 67 specimens of prostate cancer tissue and 78 specimens of prostate hyperplasia tissue were detected by immunohistochemistry,and the relationship between its expression and the Gleason grade was analysed. The content of 4- HNE of prostate cancer and prostate hyperplasia patients and free prostate disease elderly men were detected and compared by ELISA. Results: The expression postive rates of 4-HNE in prostatic cancer and prostatic hyperplasia were 100. 00% and 2. 56% respecively, and their difference had statistical significance(P ＜ 0. 01) . There was no correlation between the expression of 4-HNE and Gleason grade(P ＞ 0. 05) . The contents of 4-HNE in the serums of prostate cancer patients were obviously higher than those of prostate hyperplasia and free prostate disease patients(P ＜ 0. 01) . Conclusions: The expression levels of 4-HNE are high in prostate cancer tissue and serum of patient. 4-HNE may play an important role on the mechanism of prostate cancer happens,which can be used to diagnose prostate cancer.
2012, 36(10): 1189-1190.
Abstract:
Objective: To investigate the effects of acellular allogeneic dermis scaffold and autologous skin composite transplantation on repairing skin defects resulting from burn injury or scar removal on skin appearance and observe the functions after burn repair and scar revision. Methods: Six wounds resulted from escharectomy in 4 cases of burns and 5 wounds of scar revision in 3 cases were applied with acellular allogeneic dermis scaffold and autologous split-thickness skin composite transplantationon. Results: All the 11 wounds were repaired with this method,healed primary and the skin grew well. After following-up for 4 to 6 months, there was no apparent scar contracture, and the skin had a good elasticity, appearance and functions were all satisfactory. Conclusions: The wounds of burn repair and scar revision healed well and the skin appearance and functions were satisfactory after the application of acellular allogeneic dermis scaffold and autologous skin composite transplantation.
Objective: To investigate the effects of acellular allogeneic dermis scaffold and autologous skin composite transplantation on repairing skin defects resulting from burn injury or scar removal on skin appearance and observe the functions after burn repair and scar revision. Methods: Six wounds resulted from escharectomy in 4 cases of burns and 5 wounds of scar revision in 3 cases were applied with acellular allogeneic dermis scaffold and autologous split-thickness skin composite transplantationon. Results: All the 11 wounds were repaired with this method,healed primary and the skin grew well. After following-up for 4 to 6 months, there was no apparent scar contracture, and the skin had a good elasticity, appearance and functions were all satisfactory. Conclusions: The wounds of burn repair and scar revision healed well and the skin appearance and functions were satisfactory after the application of acellular allogeneic dermis scaffold and autologous skin composite transplantation.
2012, 36(10): 1191-1192,1197.
Abstract:
Objective: To explore the diagnosis and treatment of blunt pancreatic injury. Methods: The clinical data of 32 cases with blunt pancreatic injury were retrospectively analyzed from Jan 2005 to Jul 2011. Among 32 cases,17 and 15 cases were diagnosed throngh operation and preoperative examination of B-ultrasonography and multi-layer CT respectively. Five cases with nonoperative treatment,5 cases with the tail of pancreas and spleen resecting, 15 cases with pancreatic debridement drainage,2 cases with duodenal diverticulization,4 cases with pancreatic head end embedding and far end jejunum Roux-en-Y anastomosis and 1 case with pancreaticoduodenal resection were implemented. Results: Thirty cases were cured and the cure rate was 93. 75%. Two cases died and the fatality rate was 6. 25%,which were caused by hemorrhage, infection,multiple organ failure and important organ injury. Three cases with pancreatic leakage were found and two cases cured by conservative treatment and 1 case cured by operating. Two cases of 4 cases with pancreas pseudocyst were self-healing,Its cured by puncturing drainage and operation. Conclusions: The multi-layer CT examination and exploratory laparotomy are the important method for diagnosis of pancreatic injury. The site and degrees of pancreatic injury and pancreatic duct damage are the important basis of treatment and the proper perioperative management can reduce the complications and fatality rate.
Objective: To explore the diagnosis and treatment of blunt pancreatic injury. Methods: The clinical data of 32 cases with blunt pancreatic injury were retrospectively analyzed from Jan 2005 to Jul 2011. Among 32 cases,17 and 15 cases were diagnosed throngh operation and preoperative examination of B-ultrasonography and multi-layer CT respectively. Five cases with nonoperative treatment,5 cases with the tail of pancreas and spleen resecting, 15 cases with pancreatic debridement drainage,2 cases with duodenal diverticulization,4 cases with pancreatic head end embedding and far end jejunum Roux-en-Y anastomosis and 1 case with pancreaticoduodenal resection were implemented. Results: Thirty cases were cured and the cure rate was 93. 75%. Two cases died and the fatality rate was 6. 25%,which were caused by hemorrhage, infection,multiple organ failure and important organ injury. Three cases with pancreatic leakage were found and two cases cured by conservative treatment and 1 case cured by operating. Two cases of 4 cases with pancreas pseudocyst were self-healing,Its cured by puncturing drainage and operation. Conclusions: The multi-layer CT examination and exploratory laparotomy are the important method for diagnosis of pancreatic injury. The site and degrees of pancreatic injury and pancreatic duct damage are the important basis of treatment and the proper perioperative management can reduce the complications and fatality rate.
2012, 36(10): 1193-1997.
Abstract:
Objective: To investigate the expression of integrin 3 and Ki-67 in esophageal carcinoma and its relation to the recurrence and metastasis of tumor. Methods: The expression of integrin 3 and Ki-67 of the cases that survived over 5 years without recurrence or metastasis(group A) and died of recurrence or metastasis within 1 year(group B) were detected by immunohistochemistry. Results: No expression or little expression of integrin 3 of normal esophageal mucosa were detected,but varying degrees of expression in esophageal carcinoma. All tissue expressed Ki-67,which were found at the basilar part of normal squamous epithelium with express strength less than 1 + and esophageal carcinoma tissue. The integrin 3 and Ki-67 expressions of group B were significantly higher that of the group A(P ＜ 0. 01) . The expression of integrin 3 in ESCC had interrelation with the tumor length,T stage and lymph metastasis(P ＜ 0. 05 to P ＜ 0. 01) . The expression of Ki-67 had interrelation with tumor differentiation and lymph metastasis (P ＜ 0. 05 and P ＜ 0. 01) . Conclusions: The expression of integrin 3 in esophageal squmous cell carcinoma is a significant marker in predicting the recurrence or metastasis of tumor,which combined with Ki-67 may be of prognostic value to predict the recurrence or metastasis of esophageal carcinoma.
Objective: To investigate the expression of integrin 3 and Ki-67 in esophageal carcinoma and its relation to the recurrence and metastasis of tumor. Methods: The expression of integrin 3 and Ki-67 of the cases that survived over 5 years without recurrence or metastasis(group A) and died of recurrence or metastasis within 1 year(group B) were detected by immunohistochemistry. Results: No expression or little expression of integrin 3 of normal esophageal mucosa were detected,but varying degrees of expression in esophageal carcinoma. All tissue expressed Ki-67,which were found at the basilar part of normal squamous epithelium with express strength less than 1 + and esophageal carcinoma tissue. The integrin 3 and Ki-67 expressions of group B were significantly higher that of the group A(P ＜ 0. 01) . The expression of integrin 3 in ESCC had interrelation with the tumor length,T stage and lymph metastasis(P ＜ 0. 05 to P ＜ 0. 01) . The expression of Ki-67 had interrelation with tumor differentiation and lymph metastasis (P ＜ 0. 05 and P ＜ 0. 01) . Conclusions: The expression of integrin 3 in esophageal squmous cell carcinoma is a significant marker in predicting the recurrence or metastasis of tumor,which combined with Ki-67 may be of prognostic value to predict the recurrence or metastasis of esophageal carcinoma.
2012, 36(10): 1198-1201.
Abstract:
Objective: To explore the influence and clinical significance of cyclindependent kinase inhibitor 2A (CDKN2A) inactivation on small cell lung cancer(SCLC) . Methods: The gene and protein expression of CDKN2A in SCLC tissue of 72 cases and non-tumor lung tissue of 68 cases were detected by the semi-quantitative RT-PCR, IHC and Western blot. Results: The CDKN2A and its first and second exon mRNA expression of SCLC were significantly less than that of non-tumor lung tissue,and there was statistical significance between their diffenence(P ＜ 0. 01) . The protein expression rate of CDKN2A in SCLC(68. 1%) was significantly higher than that of non-tumor lung tissue(36. 8%) (P ＜ 0. 01) . The protein expression of CDKN2A in SCLC was significantly lower than that of non-tumor lung tissue(P ＜ 0. 05) . There was not statistical significance between the CDKN2A expression in different pathological stages and lymph node metastasis of SCLC tissue (P ＞ 0. 05) . Conclusions: The CDKN2A gene mutation and its encoded protein inactivation are related to the development and progression of SCLC.
Objective: To explore the influence and clinical significance of cyclindependent kinase inhibitor 2A (CDKN2A) inactivation on small cell lung cancer(SCLC) . Methods: The gene and protein expression of CDKN2A in SCLC tissue of 72 cases and non-tumor lung tissue of 68 cases were detected by the semi-quantitative RT-PCR, IHC and Western blot. Results: The CDKN2A and its first and second exon mRNA expression of SCLC were significantly less than that of non-tumor lung tissue,and there was statistical significance between their diffenence(P ＜ 0. 01) . The protein expression rate of CDKN2A in SCLC(68. 1%) was significantly higher than that of non-tumor lung tissue(36. 8%) (P ＜ 0. 01) . The protein expression of CDKN2A in SCLC was significantly lower than that of non-tumor lung tissue(P ＜ 0. 05) . There was not statistical significance between the CDKN2A expression in different pathological stages and lymph node metastasis of SCLC tissue (P ＞ 0. 05) . Conclusions: The CDKN2A gene mutation and its encoded protein inactivation are related to the development and progression of SCLC.
2012, 36(10): 1201-1202.
Abstract:
Objective: To investigate the effects and clinical significance of early minimal feeding and non-nutritive sucking for very low birth weight infants(VLBWI) to prevent. feeding intolerance. Methods: Sixty-nine cases of VLBWI were randomly divided into treatment group(30 cases) ,who were given early minimal feeding and non-nutritive sucking, and control group(39 cases) ,who were given the traditional method to feed. The time of recovering to birth weight, reaching whole enteral feeding and meconium emptying, and the number of cases of feeding intolerance were recorded. Results: In treatment group, the time of recovering birth weight, reaching whole enteral feeding and meconium emptying were shorter and the rates of feeding intolerance were lower than control group(P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: Early feeding intervention can promote intestinal mature, reduce the feeding problems,and improve the nutritional status of very low birth weight infants.
Objective: To investigate the effects and clinical significance of early minimal feeding and non-nutritive sucking for very low birth weight infants(VLBWI) to prevent. feeding intolerance. Methods: Sixty-nine cases of VLBWI were randomly divided into treatment group(30 cases) ,who were given early minimal feeding and non-nutritive sucking, and control group(39 cases) ,who were given the traditional method to feed. The time of recovering to birth weight, reaching whole enteral feeding and meconium emptying, and the number of cases of feeding intolerance were recorded. Results: In treatment group, the time of recovering birth weight, reaching whole enteral feeding and meconium emptying were shorter and the rates of feeding intolerance were lower than control group(P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: Early feeding intervention can promote intestinal mature, reduce the feeding problems,and improve the nutritional status of very low birth weight infants.
2012, 36(10): 1203-1205.
Abstract:
Objective: To compare the effects of esomeprazole and omemeprazole of proton pump inhibitors on gastric acid secretion and esophageal inflammation control of patients with gastro-esophageal reflux disease(GERD) . Methods: Sixty-four cases with GERD were randomly divided into A group which were treated with oral 40 mg esomeprazole and B group which were treated with oral 40 mg omemeprazole once a day for 4 weeks. Results: The total effective rates of symptom remission of group A and B were 96. 6% and 77. 1%, respectively,which had no statistical differences(P ＞ 0. 05) ; the cure rates of csophagitis of group A and B were 93. 1% and 65. 7%, respectively,which had statistical differences(P ＜0. 05) . Conclusions: The cure rates of esomepraole are better than that of omemeprazole.
Objective: To compare the effects of esomeprazole and omemeprazole of proton pump inhibitors on gastric acid secretion and esophageal inflammation control of patients with gastro-esophageal reflux disease(GERD) . Methods: Sixty-four cases with GERD were randomly divided into A group which were treated with oral 40 mg esomeprazole and B group which were treated with oral 40 mg omemeprazole once a day for 4 weeks. Results: The total effective rates of symptom remission of group A and B were 96. 6% and 77. 1%, respectively,which had no statistical differences(P ＞ 0. 05) ; the cure rates of csophagitis of group A and B were 93. 1% and 65. 7%, respectively,which had statistical differences(P ＜0. 05) . Conclusions: The cure rates of esomepraole are better than that of omemeprazole.
2012, 36(10): 1205-1206.
Abstract:
Objective: To explore the application value of nasal tracheal cannula guided by light wand in critical patients in ICU. Methods: The front of metal wire was equipmented with a light-emitting light bulbs,batteries were produced after the termination of a light wand. Endotracheal tube will be set in light stick along slowly, and inserted into the nasal cavity to anterior cricothyroid membrane with ray bright spot for the seeker, then insert the trachea, remove the light stick, inflate airbags. Results: The smooth tubs were in place in 25-60 seconds in 68 cases, all patients were carried out to achieve prominent respiratory purpose of artificial ventilation,had a good tolerance and safety performance, as well as the requirements of a long time inserted tube with no special complications. Conclusions: Light wand guided nasal intubation can be fast, simple and safe to create artificial airway, the success rate is high, it has an important clinical value.
Objective: To explore the application value of nasal tracheal cannula guided by light wand in critical patients in ICU. Methods: The front of metal wire was equipmented with a light-emitting light bulbs,batteries were produced after the termination of a light wand. Endotracheal tube will be set in light stick along slowly, and inserted into the nasal cavity to anterior cricothyroid membrane with ray bright spot for the seeker, then insert the trachea, remove the light stick, inflate airbags. Results: The smooth tubs were in place in 25-60 seconds in 68 cases, all patients were carried out to achieve prominent respiratory purpose of artificial ventilation,had a good tolerance and safety performance, as well as the requirements of a long time inserted tube with no special complications. Conclusions: Light wand guided nasal intubation can be fast, simple and safe to create artificial airway, the success rate is high, it has an important clinical value.
2012, 36(10): 1207-1209.
Abstract:
Objective: To explore the clinical effects of artificial femoral head replacement in treatment of senile patients with intertrochanteric fracture. Methods: Thirty senile patients with intertrochanteric fracture were treated with artificial femoral head replacement,which were fixed with steel wire after fracture restoration to recover the intertrochanteric anatomy relationship by the lateral incision of hip joint. The artificial bone cement femoral head were placed and antibiotics were applied at postoperation. The hip jiont function were exercised at 1 week and began to walk at 2 weeks postoperation. Results: All surgeries were successful without death and failure cases. Fracture restoration was good and the basic life of the patients were taken care of oneself,which effective rates were of 86. 7% after a follow-up of from 12 to 24 months. Conclusions: Treatment of senile patients with intertrochanteria femoral fractures with the artificial femoral head placement can shorten the time of patients staying in bed. Early functional exercise of hip jiont can reduce complications and curative effects are reliable.
Objective: To explore the clinical effects of artificial femoral head replacement in treatment of senile patients with intertrochanteric fracture. Methods: Thirty senile patients with intertrochanteric fracture were treated with artificial femoral head replacement,which were fixed with steel wire after fracture restoration to recover the intertrochanteric anatomy relationship by the lateral incision of hip joint. The artificial bone cement femoral head were placed and antibiotics were applied at postoperation. The hip jiont function were exercised at 1 week and began to walk at 2 weeks postoperation. Results: All surgeries were successful without death and failure cases. Fracture restoration was good and the basic life of the patients were taken care of oneself,which effective rates were of 86. 7% after a follow-up of from 12 to 24 months. Conclusions: Treatment of senile patients with intertrochanteria femoral fractures with the artificial femoral head placement can shorten the time of patients staying in bed. Early functional exercise of hip jiont can reduce complications and curative effects are reliable.
2012, 36(10): 1228-1229.
Abstract:
Objective: To investigate the significance of molybdenum target in inspecting breast hypoechoic mass. Methods: Sixty-five cases with breast hypoechoic mass diagnosed by ultrasound were detected by mammography of molybdenum target. Results: Seventeen cases with non-clear lesions and 23 cases with breast hyperplastic mass were regularly followed-up. Twenty-five cases were opertated, which included 15 cases of breast cancer,5 cases of fibroadenoma,3 cases of breast adenosis,1 case of papilloma and 1 case of fibrous tissue proliferation complicated with inflammatory cell infiltration. The diagnostic accuracy rate of the dense type,many and few gland type of breast cancer was 43% and the diagnostic accuracy rate of the degeneration type of breast cancer was 82%. The clustered minute calcification was found in 7 cases of 25 cases. Conclusions: The molybdenum target examination for breast hypoechoic mass can improve the diagnostic accuracy rate of mass.
Objective: To investigate the significance of molybdenum target in inspecting breast hypoechoic mass. Methods: Sixty-five cases with breast hypoechoic mass diagnosed by ultrasound were detected by mammography of molybdenum target. Results: Seventeen cases with non-clear lesions and 23 cases with breast hyperplastic mass were regularly followed-up. Twenty-five cases were opertated, which included 15 cases of breast cancer,5 cases of fibroadenoma,3 cases of breast adenosis,1 case of papilloma and 1 case of fibrous tissue proliferation complicated with inflammatory cell infiltration. The diagnostic accuracy rate of the dense type,many and few gland type of breast cancer was 43% and the diagnostic accuracy rate of the degeneration type of breast cancer was 82%. The clustered minute calcification was found in 7 cases of 25 cases. Conclusions: The molybdenum target examination for breast hypoechoic mass can improve the diagnostic accuracy rate of mass.
2012, 36(10): 1232-1234.
Abstract:
Objective: To analyze the KPC genotype in imipenem resistant-Klebsiella pneumoniae. Methods: The modified Hodge test (MHT) was used to screen the KPC enzyme-producing Klebsiella pneumoniae; the KPC gene was confirmed by PCR and DNA sequencing, and the KPC genotype was further confirmed by comparison with GenBank from internet. Homology of KPC was analyzed by using pulsed-field gel electrophoresis(PFGE) . Results: Ten of the 13 strains(76. 92%) of imipenem resistant-Klebsiella pneumoniae were proved positive by MHT,2 (15. 38%) of which presented positive KPC gene; and the KPC gene was confirmed as type-2 by comparision with GenBank. The Results of PFGE demonstrated that the 2 strains came from the same clone. Conclusions: KPC-2 type carbapenemases gene in Klebsiella pneumoniae has been isolated.
Objective: To analyze the KPC genotype in imipenem resistant-Klebsiella pneumoniae. Methods: The modified Hodge test (MHT) was used to screen the KPC enzyme-producing Klebsiella pneumoniae; the KPC gene was confirmed by PCR and DNA sequencing, and the KPC genotype was further confirmed by comparison with GenBank from internet. Homology of KPC was analyzed by using pulsed-field gel electrophoresis(PFGE) . Results: Ten of the 13 strains(76. 92%) of imipenem resistant-Klebsiella pneumoniae were proved positive by MHT,2 (15. 38%) of which presented positive KPC gene; and the KPC gene was confirmed as type-2 by comparision with GenBank. The Results of PFGE demonstrated that the 2 strains came from the same clone. Conclusions: KPC-2 type carbapenemases gene in Klebsiella pneumoniae has been isolated.
2012, 36(10): 1235-1237.
Abstract:
Objective: To study the clinical significance of plasma D-dimer and fibrinogen levels in diagnosis of malignancy. Methods: The plasma D-dimer and fibrinogen levels were tested in 60 common malignancy patients and 30 healthy cases as control by German Sysmex CA-1500 auto coagulative instrument. Results: Compared with control group, the plasma D-dimer and fibrinogen levels in malignancy patients' were increased obviously(P ＜ 0. 01) . There was no statistical difference about plasma D-dimer and fibrinogen positive rates among the common five cancers(P ＞ 0. 05) ,but had significant difference with control group(P ＜ 0. 05) . Conclusions: Malignancy patients have the secondary different degree of fibrinolysis,plasma D-dimer and fibrinogen levels are able to facilitate the diagnosis of malignancy.
Objective: To study the clinical significance of plasma D-dimer and fibrinogen levels in diagnosis of malignancy. Methods: The plasma D-dimer and fibrinogen levels were tested in 60 common malignancy patients and 30 healthy cases as control by German Sysmex CA-1500 auto coagulative instrument. Results: Compared with control group, the plasma D-dimer and fibrinogen levels in malignancy patients' were increased obviously(P ＜ 0. 01) . There was no statistical difference about plasma D-dimer and fibrinogen positive rates among the common five cancers(P ＞ 0. 05) ,but had significant difference with control group(P ＜ 0. 05) . Conclusions: Malignancy patients have the secondary different degree of fibrinolysis,plasma D-dimer and fibrinogen levels are able to facilitate the diagnosis of malignancy.
2012, 36(10): 1237-1239.
Abstract:
Objective: To explore the correlation of iron deficiency anemia (IDA) of months to 6 years old children in Huaibei. Methods: Trace element iron and the parameters of hemoglobin and red blood cell were detcected by atomic absorption method and three classification of blood cell analyzer respectively. Results: The incidence of anemia and iron deficiency of different ages had statistical significance(P ＜ 0. 01) , and with the age increasing, the incidence showed a decreasing trend(P ＜ 0. 05) ,but no statistical significance for different sex of the same age(P ＞ 0. 05) . The incidence of IDA and recessive IDA of all cases were 8. 0% and 5. 4% respectively. The incidence of IDA in rural area was significant higher than that in urban area(8. 20% versus 2. 55%,P ＜ 0. 01) . Conclusions: The measure of trace element iron can be used as screen test for the early diagnosis of IDA, especially for recessive IDA. Actively adopting comprehensive intervention measures and strengthening nutrition education can improve children＇s health levels.
Objective: To explore the correlation of iron deficiency anemia (IDA) of months to 6 years old children in Huaibei. Methods: Trace element iron and the parameters of hemoglobin and red blood cell were detcected by atomic absorption method and three classification of blood cell analyzer respectively. Results: The incidence of anemia and iron deficiency of different ages had statistical significance(P ＜ 0. 01) , and with the age increasing, the incidence showed a decreasing trend(P ＜ 0. 05) ,but no statistical significance for different sex of the same age(P ＞ 0. 05) . The incidence of IDA and recessive IDA of all cases were 8. 0% and 5. 4% respectively. The incidence of IDA in rural area was significant higher than that in urban area(8. 20% versus 2. 55%,P ＜ 0. 01) . Conclusions: The measure of trace element iron can be used as screen test for the early diagnosis of IDA, especially for recessive IDA. Actively adopting comprehensive intervention measures and strengthening nutrition education can improve children＇s health levels.
2012, 36(10): 1240-1244.
Abstract:
Objective: To explore the influential factors of the medical college students' attitude on suicide and provide theoretical evidence for change of the students' negative attitude. Methods: In a method of random sampling,3 030 medical students of lower grade were chosen to conduct a questionnaire investigation by using suicide attitude scale,suicide ideation scale and Beck depression questionnaire. Results: Medical college students kept a positive perception toward the discrimination against suicide(29. 12 14. 13 marks) and social importance of suicide(25. 34 15. 57 marks) ,and kept a negative perception toward the unpreventable of suicide (71. 52 13. 82 marks) ,uncontrolled of suicide(65. 01 18. 72 marks) ,positivism of suicide(64. 65 17. 35 marks) and the extent of suicide on changing others' behaviors(65. 81 15. 24 marks) ; while they were neutral on how much the attempted suicide was like the completed suicide(53. 70 21. 48 marks) . Single factor analysis revealed that the factors which influenced all subscales of suicide attitudes were different. The Results of multiple linear regression showed that family function was a protective element while suicide ideation and depression were dangerous elements. Conclusions: Relevant Methods should be taken to change the medical college students' negative attitude on suicide, so as to dispel any injury.
Objective: To explore the influential factors of the medical college students' attitude on suicide and provide theoretical evidence for change of the students' negative attitude. Methods: In a method of random sampling,3 030 medical students of lower grade were chosen to conduct a questionnaire investigation by using suicide attitude scale,suicide ideation scale and Beck depression questionnaire. Results: Medical college students kept a positive perception toward the discrimination against suicide(29. 12 14. 13 marks) and social importance of suicide(25. 34 15. 57 marks) ,and kept a negative perception toward the unpreventable of suicide (71. 52 13. 82 marks) ,uncontrolled of suicide(65. 01 18. 72 marks) ,positivism of suicide(64. 65 17. 35 marks) and the extent of suicide on changing others' behaviors(65. 81 15. 24 marks) ; while they were neutral on how much the attempted suicide was like the completed suicide(53. 70 21. 48 marks) . Single factor analysis revealed that the factors which influenced all subscales of suicide attitudes were different. The Results of multiple linear regression showed that family function was a protective element while suicide ideation and depression were dangerous elements. Conclusions: Relevant Methods should be taken to change the medical college students' negative attitude on suicide, so as to dispel any injury.
2012, 36(10): 1244-1246,1249.
Abstract:
Objective: To know the psychological experience of during diagnosis of 21 cases with breast cancer and provide a theoretical basis and reference for clinical nursing. Methods: Twenty-one cases with suspected breast cancer were interviewed through the phenomenological research method. The information of interview was analysed,sorted in according to Colaizzi' s analysis,and different themes were extracted. Results: Research showed that the psychological experience of all cases with breast cancer patients has a lot of in common,but there were still differences during their diagnosis. These were not timely seek medical advice,the shock of the illness,desire of life and chances,anxiety and fear,the demand of family love. Conclusions: Doctor need understand a series of psychological changes of the patients and tell the related knowledge of disease. At the same time,patient can obtain the moral support from family,especially husband as far as possible,which can help patients recast the life confidence.
Objective: To know the psychological experience of during diagnosis of 21 cases with breast cancer and provide a theoretical basis and reference for clinical nursing. Methods: Twenty-one cases with suspected breast cancer were interviewed through the phenomenological research method. The information of interview was analysed,sorted in according to Colaizzi' s analysis,and different themes were extracted. Results: Research showed that the psychological experience of all cases with breast cancer patients has a lot of in common,but there were still differences during their diagnosis. These were not timely seek medical advice,the shock of the illness,desire of life and chances,anxiety and fear,the demand of family love. Conclusions: Doctor need understand a series of psychological changes of the patients and tell the related knowledge of disease. At the same time,patient can obtain the moral support from family,especially husband as far as possible,which can help patients recast the life confidence.
2012, 36(10): 1247-1249.
Abstract:
Objective: To investigate the related factors and preventive measures for nosocomial infection(NI) in the Department of Geriatrics. Methods: The clinical data of 673 cases of elderly inpatients were analyzed. The single factor analysis and logistic regression analysis about the variable quantity of the influence factors which induced NI were performed. Results: Among the 673 inpatients,52 patients were gotten NI. The most common NI usually was occurred in lung,upper respiratory tract, gastrointestinal or urinary tract. In the related examination for variable quantity of single factor, the time of staying in hospital, age,diabetes, cerebral vascular disease and invasive manipulation were the independent dangerous factors of NI. The staying time in hospital was the most improtant factor. Conclusions: The time of staying in hospital,age,diabetes,cerebral vascular disease and invasive manipulation were the independent dangerous factors that contribute to NI of inpatients. Prevention and cure of these factors should be emphasized to decrease the incidence of NI in elderly inpatients.
Objective: To investigate the related factors and preventive measures for nosocomial infection(NI) in the Department of Geriatrics. Methods: The clinical data of 673 cases of elderly inpatients were analyzed. The single factor analysis and logistic regression analysis about the variable quantity of the influence factors which induced NI were performed. Results: Among the 673 inpatients,52 patients were gotten NI. The most common NI usually was occurred in lung,upper respiratory tract, gastrointestinal or urinary tract. In the related examination for variable quantity of single factor, the time of staying in hospital, age,diabetes, cerebral vascular disease and invasive manipulation were the independent dangerous factors of NI. The staying time in hospital was the most improtant factor. Conclusions: The time of staying in hospital,age,diabetes,cerebral vascular disease and invasive manipulation were the independent dangerous factors that contribute to NI of inpatients. Prevention and cure of these factors should be emphasized to decrease the incidence of NI in elderly inpatients.
2012, 36(10): 1250-1252.
Abstract:
Objective: To investigate the effects of psychological intervention on sexual function and quality of patients treated with vaginoplasty. Methods: Forty-two cases with vaginoplasty were divided into the control group(20 cases) ,which was treated with the conventional perioperative care and discharge guidance, and the intervention group(22 cases) ,which was treated with added 12 months of comprehensive psychological behavior intervention. At 3,6 and 12 months post-operation, the effects of nuring of all cases were investigated according to sexual Quality of Life Questionnaire for Women(SQLQ-W) and Quality of Live Brief Questionnaire(QOLBREF) . Results: The scores of the SQLQ-W and QOL-BREF of two groups were obviously enhanced at 6 and 12 months post-operation, and significant differences were found(P ＜ 0. 05-P ＜ 0. 01) . The score difference of the SQLQ-W of two groups had no statistical significance at 3 and 6 months post-operation (P ＞ 0. 05) ,while at 12 months post-operation the scores of intervention group were significant higher that of the control group. At 6 and 12 months post-operation, the scores of QOL-BREF of intervention group were higher that of the control group(P ＜ 0. 01) . Conclusions: The psychological intervention can improve the sexual function and quality of patients treated with vaginoplasty.
Objective: To investigate the effects of psychological intervention on sexual function and quality of patients treated with vaginoplasty. Methods: Forty-two cases with vaginoplasty were divided into the control group(20 cases) ,which was treated with the conventional perioperative care and discharge guidance, and the intervention group(22 cases) ,which was treated with added 12 months of comprehensive psychological behavior intervention. At 3,6 and 12 months post-operation, the effects of nuring of all cases were investigated according to sexual Quality of Life Questionnaire for Women(SQLQ-W) and Quality of Live Brief Questionnaire(QOLBREF) . Results: The scores of the SQLQ-W and QOL-BREF of two groups were obviously enhanced at 6 and 12 months post-operation, and significant differences were found(P ＜ 0. 05-P ＜ 0. 01) . The score difference of the SQLQ-W of two groups had no statistical significance at 3 and 6 months post-operation (P ＞ 0. 05) ,while at 12 months post-operation the scores of intervention group were significant higher that of the control group. At 6 and 12 months post-operation, the scores of QOL-BREF of intervention group were higher that of the control group(P ＜ 0. 01) . Conclusions: The psychological intervention can improve the sexual function and quality of patients treated with vaginoplasty.
2012, 36(10): 1253-1255.
Abstract:
Objective: To reduce or avoid the incidence of pressure sore through strengthening quality control. Methods: The threelevel quality control system of pressure sore was establish, the process of evaluation, report and treatment were regulated,and the management was standardized for pressure sore patients. Results: Through the improvement of three-level quality control system, hospitalized patients reporting high-risk pressure ulcer rate, the effective return rate, improved or cured rate higher than the standardized management(P ＜ 0. 01) , it was inevitable pressure ulcer incidence is lower than specification management(P ＜ 0. 01) , intopressure sores were no significant differences before and after(P ＞ 0. 05) . Conclusions: The implementation of three-level quality control and systematization and standardization of pressure sore management can provide guarantee for the prevention of pressure sore.
Objective: To reduce or avoid the incidence of pressure sore through strengthening quality control. Methods: The threelevel quality control system of pressure sore was establish, the process of evaluation, report and treatment were regulated,and the management was standardized for pressure sore patients. Results: Through the improvement of three-level quality control system, hospitalized patients reporting high-risk pressure ulcer rate, the effective return rate, improved or cured rate higher than the standardized management(P ＜ 0. 01) , it was inevitable pressure ulcer incidence is lower than specification management(P ＜ 0. 01) , intopressure sores were no significant differences before and after(P ＞ 0. 05) . Conclusions: The implementation of three-level quality control and systematization and standardization of pressure sore management can provide guarantee for the prevention of pressure sore.
2012, 36(10): 1255-1257.
Abstract:
Objective: To investigate the effects of retention enema modified on ulcerative colitis. Methods: Thirty patients with ulcerative colitis were randomly divided into observation group(15 cases) and control group(15 cases) . The control group were treated with traditional retention enema and the observation group with retention enema modified. The therapeutic effects of two groups were observed. Results: The retention time and therapeutic efficacy of rectal solution in observation group were better than that of the control group(P ＜ 0. 01) . The hospital stays of two groups had not statistical significance(P ＞ 0. 05) . Conclusions: Therapeutic efficacy of retention enema modified is good, and which is simple, economy,patient satisfaction. The method can be easily mastered and operated.
Objective: To investigate the effects of retention enema modified on ulcerative colitis. Methods: Thirty patients with ulcerative colitis were randomly divided into observation group(15 cases) and control group(15 cases) . The control group were treated with traditional retention enema and the observation group with retention enema modified. The therapeutic effects of two groups were observed. Results: The retention time and therapeutic efficacy of rectal solution in observation group were better than that of the control group(P ＜ 0. 01) . The hospital stays of two groups had not statistical significance(P ＞ 0. 05) . Conclusions: Therapeutic efficacy of retention enema modified is good, and which is simple, economy,patient satisfaction. The method can be easily mastered and operated.
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