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2017 Vol. 42, No. 1
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2017, 42(1): 3-7,12.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.002
Abstract:
Objective: To investigate the competency and job performance status of clinical nurses in general hospitals,analyze the correlation,and explore the influencing factors of job performance.Methods: Four hundred registered nurses working more than 1 year from 4 tertiary general hospitals in a city were investigated by cluster sampling questionnaire using the Questionnaire of Nurses' Competency and Scale of Nurses' Job Performance.Results: The average scores of the competency and job performance in clinical nurses were (3.70±0.47) and (4.02±0.54),respectively.The univariate analysis of variance indicated that the differences of the competency and job performance in different age,department,title,duty,nature of employment,work experience,initial education and marital status clinical nurses were statistically significant(P<0.05 to P<0.01).The results of pearson liner correlation analysis showed that the overall competency and 14 competent characteristics were positively correlated with the job performance and its 3 dimensions(P<0.01).Stepwise regression analysis showed that the important inflencing variables on the job performance of clinical nurses were the working year,moral responsibility,compassion and moral responsibility in turn(P<0.01).Conclusions: Clinical nurses' competency is one of the important influencing factor on job performance.Nursing managers should implemented the specific measure to the influencing factor of job performance,and cluture the needs of the competency of clinical nurses in order to further improve the job performance.
Objective: To investigate the competency and job performance status of clinical nurses in general hospitals,analyze the correlation,and explore the influencing factors of job performance.Methods: Four hundred registered nurses working more than 1 year from 4 tertiary general hospitals in a city were investigated by cluster sampling questionnaire using the Questionnaire of Nurses' Competency and Scale of Nurses' Job Performance.Results: The average scores of the competency and job performance in clinical nurses were (3.70±0.47) and (4.02±0.54),respectively.The univariate analysis of variance indicated that the differences of the competency and job performance in different age,department,title,duty,nature of employment,work experience,initial education and marital status clinical nurses were statistically significant(P<0.05 to P<0.01).The results of pearson liner correlation analysis showed that the overall competency and 14 competent characteristics were positively correlated with the job performance and its 3 dimensions(P<0.01).Stepwise regression analysis showed that the important inflencing variables on the job performance of clinical nurses were the working year,moral responsibility,compassion and moral responsibility in turn(P<0.01).Conclusions: Clinical nurses' competency is one of the important influencing factor on job performance.Nursing managers should implemented the specific measure to the influencing factor of job performance,and cluture the needs of the competency of clinical nurses in order to further improve the job performance.
2017, 42(1): 8-12.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.003
Abstract:
Objective: To investigate the effects of the subjective well-being and organizational identification on the job performance in nurses of tertiary hospital.Methods: Two hundred and forty-two nurses from tertiary hospital were investigated using the self-compiled general information questionnaire,subjective well-being scale,organizational identification scale and job performance scale,the result of which was descriptively and relatively analyzed,and constructed the structural equation model.Results: The total scores of the subjective well-being scale,organizational identification scale and job performance scale were(75.27±10.49),(31.83±5.27) and(74.19±8.71),respectively.The dimension scores from high to down of the relationship performance,learning performance,task performance and innovation performance were(19.36±2.50),(18.48±2.65),(18.31±2.33) and(18.19±3.20),respectively.The subjective well-being was not related to the task performance(P>0.05).There were the positive relationship between subjective well-being,organizational identification and job performance(P<0.05).The structural equation model showed that the subjective well-being and organizational identification had a direct effect on job performance,and with organizational identification as an intermediary variable,the subjective well-being had a direct effect on job performance.The standardized path coefficients of the subjective well-being and organizational identification to the job performance were 0.30 and 0.33,respectively,and the standardized total effect of the subjective well-being on the performance was 0.39,and the exogenous variable of subjective well-being and organizational identification could explain 25% of the total variation of the internal variable job performance.Conclusions: The subjective well-being,job involvement and job performance in nurses of tertiary hospital is optimistic,but which still has the improvement room.The hospital and nursing administrators should improve the subjective well-being and organizational identification of nurse to increase job performance.
Objective: To investigate the effects of the subjective well-being and organizational identification on the job performance in nurses of tertiary hospital.Methods: Two hundred and forty-two nurses from tertiary hospital were investigated using the self-compiled general information questionnaire,subjective well-being scale,organizational identification scale and job performance scale,the result of which was descriptively and relatively analyzed,and constructed the structural equation model.Results: The total scores of the subjective well-being scale,organizational identification scale and job performance scale were(75.27±10.49),(31.83±5.27) and(74.19±8.71),respectively.The dimension scores from high to down of the relationship performance,learning performance,task performance and innovation performance were(19.36±2.50),(18.48±2.65),(18.31±2.33) and(18.19±3.20),respectively.The subjective well-being was not related to the task performance(P>0.05).There were the positive relationship between subjective well-being,organizational identification and job performance(P<0.05).The structural equation model showed that the subjective well-being and organizational identification had a direct effect on job performance,and with organizational identification as an intermediary variable,the subjective well-being had a direct effect on job performance.The standardized path coefficients of the subjective well-being and organizational identification to the job performance were 0.30 and 0.33,respectively,and the standardized total effect of the subjective well-being on the performance was 0.39,and the exogenous variable of subjective well-being and organizational identification could explain 25% of the total variation of the internal variable job performance.Conclusions: The subjective well-being,job involvement and job performance in nurses of tertiary hospital is optimistic,but which still has the improvement room.The hospital and nursing administrators should improve the subjective well-being and organizational identification of nurse to increase job performance.
2017, 42(1): 13-17.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.004
Abstract:
Objective: To explore the effects of the psychological contract on job performance of nurses in public hospital.Methods: Five hundred and twenty-three nurses from more than secondary public hospital were investigated using the nurses' psychological contract scale and hospital nurses' job performance rating scale.The relationship between the nurses' psychological contract and job performance was explored using the descriptive statistics analysis,pearson correlation analysis and multiple stepwise regression analysise.Results: The mean scores of the hospital responsibility and nurse responsibility in psychological contract of nurses,and the job performance of nurses were(3.16±0.88),(4.49±0.58) and(4.65±0.43),respectively.Pearson correlation analysis showed that the each dimension of the psychological contract of nurses was positively correlated with the task performance and contextual performance(P<0.05 to P<0.01),and negatively correlated with the anti-production performance(P<0.05 to P<0.01).Multiple stepwise regression analysis showed that the working year,hospital characteristics,nurse team responsibility and nurse development responsibility entered the regression equation of job performance.Conclusions: Nurses perceiving psychological contract is at the medium level,the un-satisfaction of hospital responsibility is higher than that of nurses,and the manager should pay attention to the performance of hospital responsibility.The work performance of nurses is at a relatively high level,and which is mainly affected by the nurses' responsibility in psychological contract.To establish and maintain the psychological contract is an important measure to improve the job performance of nurses.
Objective: To explore the effects of the psychological contract on job performance of nurses in public hospital.Methods: Five hundred and twenty-three nurses from more than secondary public hospital were investigated using the nurses' psychological contract scale and hospital nurses' job performance rating scale.The relationship between the nurses' psychological contract and job performance was explored using the descriptive statistics analysis,pearson correlation analysis and multiple stepwise regression analysise.Results: The mean scores of the hospital responsibility and nurse responsibility in psychological contract of nurses,and the job performance of nurses were(3.16±0.88),(4.49±0.58) and(4.65±0.43),respectively.Pearson correlation analysis showed that the each dimension of the psychological contract of nurses was positively correlated with the task performance and contextual performance(P<0.05 to P<0.01),and negatively correlated with the anti-production performance(P<0.05 to P<0.01).Multiple stepwise regression analysis showed that the working year,hospital characteristics,nurse team responsibility and nurse development responsibility entered the regression equation of job performance.Conclusions: Nurses perceiving psychological contract is at the medium level,the un-satisfaction of hospital responsibility is higher than that of nurses,and the manager should pay attention to the performance of hospital responsibility.The work performance of nurses is at a relatively high level,and which is mainly affected by the nurses' responsibility in psychological contract.To establish and maintain the psychological contract is an important measure to improve the job performance of nurses.
2017, 42(1): 18-22.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.005
Abstract:
Objective: To investigate the relationships between working pressure,coping style and job performance of clinical nurses.Methods: Six hundred nurses were investigated using the general information questionnaire,nurse working pressure scale,simple coping style scale and job performance scale,and the correlation analysis and regression analysis of which was performed.Results: The differences of the working pressure in different marital status,department and monthly income were statistically significant(P<0.05 to P<0.01).The working pressure was significantly negative correlation with the job performance(P<0.01).The working pressure was negatively correlated with the positive coping style,and significantly positive correlation with the negative coping style(P<0.01).The coping style played a regulating role in the relationship between working pressure and job performance.Conclusions: The working pressure of clinical nurses is at a moderate level,which can directly affect the job performance,and also indirectly affect the job performance through coping style.The nursing administrators can improve the work performance by alleviating the working pressure of nurses and encouraging nurses to adopt positive coping style.
Objective: To investigate the relationships between working pressure,coping style and job performance of clinical nurses.Methods: Six hundred nurses were investigated using the general information questionnaire,nurse working pressure scale,simple coping style scale and job performance scale,and the correlation analysis and regression analysis of which was performed.Results: The differences of the working pressure in different marital status,department and monthly income were statistically significant(P<0.05 to P<0.01).The working pressure was significantly negative correlation with the job performance(P<0.01).The working pressure was negatively correlated with the positive coping style,and significantly positive correlation with the negative coping style(P<0.01).The coping style played a regulating role in the relationship between working pressure and job performance.Conclusions: The working pressure of clinical nurses is at a moderate level,which can directly affect the job performance,and also indirectly affect the job performance through coping style.The nursing administrators can improve the work performance by alleviating the working pressure of nurses and encouraging nurses to adopt positive coping style.
2017, 42(1): 23-26.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.006
Abstract:
Objective: To investigate the effects of the volunteer motivation and organizational identification on the job performance of the clinical nurses in tertiary hospital.Methods: A total of 242 nurses from the tertiary hospital were investigated using the volunteer motivation scale,organizational identification scale and job performance scale.Results: Both the volunteer motivation and organizational identification were positive correlation with the job performance(P<0.05 to P<0.01).The volunteer motivation and organizational identification directly affected the job performance.The organizational identification played a mediating role in the relationship between volunteer motivation and job performance,the mediating effect of which accounted for 29.55% of the total effect in job performance.Conclusions: The job performance of clinical nurses in tertiary hospital is at moderate level.Nursing managers can enhance the job performance of nurses by improving the volunteer motivation and organizational identification.
Objective: To investigate the effects of the volunteer motivation and organizational identification on the job performance of the clinical nurses in tertiary hospital.Methods: A total of 242 nurses from the tertiary hospital were investigated using the volunteer motivation scale,organizational identification scale and job performance scale.Results: Both the volunteer motivation and organizational identification were positive correlation with the job performance(P<0.05 to P<0.01).The volunteer motivation and organizational identification directly affected the job performance.The organizational identification played a mediating role in the relationship between volunteer motivation and job performance,the mediating effect of which accounted for 29.55% of the total effect in job performance.Conclusions: The job performance of clinical nurses in tertiary hospital is at moderate level.Nursing managers can enhance the job performance of nurses by improving the volunteer motivation and organizational identification.
2017, 42(1): 27-29,33.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.007
Abstract:
Objective: To investigate the effects of transcription factors ETS-1 on the proliferation of the breast cancer cells by regulating miR-96.Methods: The expression of miR-96 in breast cancer cell MCF-7 with silencing the ETS-1 expression induced by the RNA interference technique was detected.The binding site of transcription factor ETS-1 was predicted using bioinformatics method,and validated with chromatin immune precipitation reaction.The miR-96 was transfected into breast cancer cell MCF-7,and the effect of which on the proliferation and clone information of breast cancer cells was observed.Results: The expression of miR-96 in breast cancer cells was significantly increased after inhibiting the expression of ETS-1(P<0.01).The expression of ETS-1 was regulated by the ETS-1 binding to the promoter region of miR-96.Compared with the control group,the abilities of proliferation and clone formation of MCF-7 were significantly increased after the overexpression of miR-96(P<0.01).Conclusions: ETS-1 can affect the proliferation and clone formation ability of breast cancer cells by regulating the expression of miR-96,which is expected to become a new target in the treatment of breast cancer.
Objective: To investigate the effects of transcription factors ETS-1 on the proliferation of the breast cancer cells by regulating miR-96.Methods: The expression of miR-96 in breast cancer cell MCF-7 with silencing the ETS-1 expression induced by the RNA interference technique was detected.The binding site of transcription factor ETS-1 was predicted using bioinformatics method,and validated with chromatin immune precipitation reaction.The miR-96 was transfected into breast cancer cell MCF-7,and the effect of which on the proliferation and clone information of breast cancer cells was observed.Results: The expression of miR-96 in breast cancer cells was significantly increased after inhibiting the expression of ETS-1(P<0.01).The expression of ETS-1 was regulated by the ETS-1 binding to the promoter region of miR-96.Compared with the control group,the abilities of proliferation and clone formation of MCF-7 were significantly increased after the overexpression of miR-96(P<0.01).Conclusions: ETS-1 can affect the proliferation and clone formation ability of breast cancer cells by regulating the expression of miR-96,which is expected to become a new target in the treatment of breast cancer.
2017, 42(1): 30-33.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.008
Abstract:
Objective: To explore the effects of the miR-186 target-regulating the osteopontin(OPN) expression on the osteogenic differentiation of bone marrow mesenchymal stem cells(bMSCs).Methods: The bMSCs were isolated from bone marrow,induced with BMP 2,and identified using the Alkaline Phosphatase(ALP) and Von-kossa staining.Targeting pairing relationship between miR-186 and OPN was predicted by bioinformatics,and identified with luciferase assay.After transfecting miR-186 mimics and siRNA interfering OPN gene into cells,the expressions of miR-186 and OPN were determined by real-time PCR,and the protein expression of OPN was detected using western blot.Results: The uniform distribution of bMSCs was found under light microscope,and the induced cells could be stained blue by ALP.MiRanda and TargetScan showed that miR-186 was well complementary with OPN gene.The result of luciferase assay showed that miR-186 could inhibit the expression of OPN mRNA.The results of real-time PCR and western blot showed that the over-expression of miR-186 could downregulate the expressions of OPN mRNA and protein.Conclusions: MiR-186 can inhibit the osteogenic differentiation of bMSCs by downregulating the OPN gene expression.
Objective: To explore the effects of the miR-186 target-regulating the osteopontin(OPN) expression on the osteogenic differentiation of bone marrow mesenchymal stem cells(bMSCs).Methods: The bMSCs were isolated from bone marrow,induced with BMP 2,and identified using the Alkaline Phosphatase(ALP) and Von-kossa staining.Targeting pairing relationship between miR-186 and OPN was predicted by bioinformatics,and identified with luciferase assay.After transfecting miR-186 mimics and siRNA interfering OPN gene into cells,the expressions of miR-186 and OPN were determined by real-time PCR,and the protein expression of OPN was detected using western blot.Results: The uniform distribution of bMSCs was found under light microscope,and the induced cells could be stained blue by ALP.MiRanda and TargetScan showed that miR-186 was well complementary with OPN gene.The result of luciferase assay showed that miR-186 could inhibit the expression of OPN mRNA.The results of real-time PCR and western blot showed that the over-expression of miR-186 could downregulate the expressions of OPN mRNA and protein.Conclusions: MiR-186 can inhibit the osteogenic differentiation of bMSCs by downregulating the OPN gene expression.
2017, 42(1): 34-35,56.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.009
Abstract:
Objective: To research the inhibition effects of brucea javanica oil on the expression of vascular endothelial growth factor(VEGF) in U251 glioma cells.Methods: The human U251 glioma cell was cultivated in vitro,set control group and three drug groups(the final concentration of Brucea javanica oil emulsion is 5 g/L,2.5 g/L,1.25 g/L).The content of VEGF in the supernatant of culture cells was measured using ELISA,and the change of VEGF protein in U251 glioma cell was measured using the immunocytochemistry.Results: The VEGF level in the supernatant of culture cells showed that the significant decreasing of the VEGF level was identified with the increasing of drug concentration(P<0.01).The immunocytochemistry showed that the expression of VEGF protein decreased with the dose increasing of brucea javanica oil.Conclusions: Brucea javanica oil emulsion can inhibit the expression of VEGF protein in U251 glioma cells.
Objective: To research the inhibition effects of brucea javanica oil on the expression of vascular endothelial growth factor(VEGF) in U251 glioma cells.Methods: The human U251 glioma cell was cultivated in vitro,set control group and three drug groups(the final concentration of Brucea javanica oil emulsion is 5 g/L,2.5 g/L,1.25 g/L).The content of VEGF in the supernatant of culture cells was measured using ELISA,and the change of VEGF protein in U251 glioma cell was measured using the immunocytochemistry.Results: The VEGF level in the supernatant of culture cells showed that the significant decreasing of the VEGF level was identified with the increasing of drug concentration(P<0.01).The immunocytochemistry showed that the expression of VEGF protein decreased with the dose increasing of brucea javanica oil.Conclusions: Brucea javanica oil emulsion can inhibit the expression of VEGF protein in U251 glioma cells.
2017, 42(1): 36-40,56.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.010
Abstract:
Objective: To explore the effects of Taohongsiwu decoction on the angiogenesis and PI3K/AKT signal pathway in brain tissue of rats with cerebral ischemia reperfusion injury(CIRI).Methods: Adult male SD rats were randomly divided into the sham group,model group,Huatuozaizao pill group(2.0 g/kg),and high,middle and low dose of Taohongsiwu decoction groups(2.1 g/kg,1.4 g/kg and 0.7 g/kg).The CIRI model rats were established using the modified MCAO,and successively infused for 7 days.The nerve function scoring,microvessel density(MVD) and vascular endothelial growth factor(VEGF),and levels of AKT and p-AKT in brain tissue of all groups were investigated using the Zea longa,immunohistochemistry and Western blot,respectively.Results: The nerve function injury in sham group were not found,the scores of the nerve dysfunction in Huatuozaizao pill group,and high,middle and low dose of Taohongsiwu decoction groups were significantly lower than that in model group(P<0.05 to P<0.01).the score of the nerve dysfunction in high dose of Taohongsiwu decoction group was significantly lower than that in Huatuozaizao pill group,and middle and low dose of Taohongsiwu decoction groups(P<0.05 to P<0.01).The levels of MVD and VEGF in sham group were significantly lower than those in model group(P<0.01),the levels of MVD in Huatuozaizao pill group and high,middle dose of Taohongsiwu decoction groups were significantly higher than those in model group(P<0.01),and the level of VEGF in high dose of Taohongsiwu decoction group was significantly higher than that in model group(P<0.01).The levels of MVD and p-AKT in high dose of Taohongsiwu decoction group were higher than those in Huatuozaizao pill group,and middle and low dose of Taohongsiwu decoction groups(P<0.05 to P<0.01),the level of VEGF in high dose of Taohongsiwu decoction group was lower than that in low dose of Taohongsiwu decoction group(P<0.05),and the differences of the levels of AKT between each group were not statistically significant(P>0.05).Conclusions: Taohongsiwu decoction in treating the rats with CIRI has significant effects,the mechanism of which may be involved in promoting the angiogenesis and recovering the nerve function by activating PI3K/AKT signal pathway in ischemic site of brain.
Objective: To explore the effects of Taohongsiwu decoction on the angiogenesis and PI3K/AKT signal pathway in brain tissue of rats with cerebral ischemia reperfusion injury(CIRI).Methods: Adult male SD rats were randomly divided into the sham group,model group,Huatuozaizao pill group(2.0 g/kg),and high,middle and low dose of Taohongsiwu decoction groups(2.1 g/kg,1.4 g/kg and 0.7 g/kg).The CIRI model rats were established using the modified MCAO,and successively infused for 7 days.The nerve function scoring,microvessel density(MVD) and vascular endothelial growth factor(VEGF),and levels of AKT and p-AKT in brain tissue of all groups were investigated using the Zea longa,immunohistochemistry and Western blot,respectively.Results: The nerve function injury in sham group were not found,the scores of the nerve dysfunction in Huatuozaizao pill group,and high,middle and low dose of Taohongsiwu decoction groups were significantly lower than that in model group(P<0.05 to P<0.01).the score of the nerve dysfunction in high dose of Taohongsiwu decoction group was significantly lower than that in Huatuozaizao pill group,and middle and low dose of Taohongsiwu decoction groups(P<0.05 to P<0.01).The levels of MVD and VEGF in sham group were significantly lower than those in model group(P<0.01),the levels of MVD in Huatuozaizao pill group and high,middle dose of Taohongsiwu decoction groups were significantly higher than those in model group(P<0.01),and the level of VEGF in high dose of Taohongsiwu decoction group was significantly higher than that in model group(P<0.01).The levels of MVD and p-AKT in high dose of Taohongsiwu decoction group were higher than those in Huatuozaizao pill group,and middle and low dose of Taohongsiwu decoction groups(P<0.05 to P<0.01),the level of VEGF in high dose of Taohongsiwu decoction group was lower than that in low dose of Taohongsiwu decoction group(P<0.05),and the differences of the levels of AKT between each group were not statistically significant(P>0.05).Conclusions: Taohongsiwu decoction in treating the rats with CIRI has significant effects,the mechanism of which may be involved in promoting the angiogenesis and recovering the nerve function by activating PI3K/AKT signal pathway in ischemic site of brain.
2017, 42(1): 41-43.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.011
Abstract:
Objective: To explore the mechanism of Qing Ying Hua Yu particle in the treatment of phlebitis of rabbit.Methods: The infusion phlebitis rabbit model induced by vinoerbline was established.The animals were divided into the Qing Ying Hua Yu group,Tong Sai Mai group,model group and blank group.The related indicators in 4 groups were observed.Results: The adjusting the coagulation function and reducing the fibrinogen in Qing Ying Hua Yu group were better than those in Tong Sai Mai group(P<0.05 to P<0.01).The difference of thrombogenesis between Qing Ying Hua Yu group and Tong Sai Mai group,the difference of inflammatory cell infiltration between Qing Ying Hua Yu group and model group,and the difference of drop of epithelial cells between Qing Ying Hua Yu group and blank group were statistically significant(P<0.01).Conclusions: Qing Ying Hua Yu particle can treat the superficial thrombophlebitis by repairing endothelial cells,controlling inflammation and organizing thrombus.
Objective: To explore the mechanism of Qing Ying Hua Yu particle in the treatment of phlebitis of rabbit.Methods: The infusion phlebitis rabbit model induced by vinoerbline was established.The animals were divided into the Qing Ying Hua Yu group,Tong Sai Mai group,model group and blank group.The related indicators in 4 groups were observed.Results: The adjusting the coagulation function and reducing the fibrinogen in Qing Ying Hua Yu group were better than those in Tong Sai Mai group(P<0.05 to P<0.01).The difference of thrombogenesis between Qing Ying Hua Yu group and Tong Sai Mai group,the difference of inflammatory cell infiltration between Qing Ying Hua Yu group and model group,and the difference of drop of epithelial cells between Qing Ying Hua Yu group and blank group were statistically significant(P<0.01).Conclusions: Qing Ying Hua Yu particle can treat the superficial thrombophlebitis by repairing endothelial cells,controlling inflammation and organizing thrombus.
2017, 42(1): 44-47.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.012
Abstract:
Objective: To investigate the effects of luteolin on the proliferation,differentiation,mineralization and Wnt pathway of MC3T3-E1 osteoblasts in vitro.Methods: The MC3T3-E1 osteoblasts were cultured with the 0.25 to 8.00 μmol/L of luteolin for 12,24 and 48 h in vitro.The 0.04 μmol/L of lovastatin treating cell was used as the positive control group.The proliferation,activity of alkaline phosphatase(ALP) and mineralization capacity of MC3T3-E1 osteoblasts in vitro were detected by cell counting kits-8 method,alkaline phosphatase kit and Alizarin Red S staining,respectively.The mRNA expressions of type Ⅰ collagen,osteocalcin,low density lipoprotein receptor related protein 5(Lrp5),β-catenin,osteoprotegerin and nuclear factor κB-activating factor receptor ligands were quantified by real-time reverse transcription PCR(qPCR).Results: Luteolin could improve the proliferation of MC3T3-E1 osteoblasts with time- and dose-dependence(P<0.05 to P<0.01).Luteolin could promote the activity of ALP,strengthen the mineralization capacity and upregulate the mRNA expressions of type Ⅰ collagen,osteocalcin,Lrp5,β-catenin,osteoprotegerin and nuclear factor κB-activating factor receptor ligands in MC3T3-E1s with dose-dependence in vitro.Conclusions: The 2.00,4.00 and 8.00 μmol/L of luteolin can promote the proliferation,differentiation and mineralization and the expressions pf Lrp5 and β-catenin in MC3T3-E1 osteoblasts.
Objective: To investigate the effects of luteolin on the proliferation,differentiation,mineralization and Wnt pathway of MC3T3-E1 osteoblasts in vitro.Methods: The MC3T3-E1 osteoblasts were cultured with the 0.25 to 8.00 μmol/L of luteolin for 12,24 and 48 h in vitro.The 0.04 μmol/L of lovastatin treating cell was used as the positive control group.The proliferation,activity of alkaline phosphatase(ALP) and mineralization capacity of MC3T3-E1 osteoblasts in vitro were detected by cell counting kits-8 method,alkaline phosphatase kit and Alizarin Red S staining,respectively.The mRNA expressions of type Ⅰ collagen,osteocalcin,low density lipoprotein receptor related protein 5(Lrp5),β-catenin,osteoprotegerin and nuclear factor κB-activating factor receptor ligands were quantified by real-time reverse transcription PCR(qPCR).Results: Luteolin could improve the proliferation of MC3T3-E1 osteoblasts with time- and dose-dependence(P<0.05 to P<0.01).Luteolin could promote the activity of ALP,strengthen the mineralization capacity and upregulate the mRNA expressions of type Ⅰ collagen,osteocalcin,Lrp5,β-catenin,osteoprotegerin and nuclear factor κB-activating factor receptor ligands in MC3T3-E1s with dose-dependence in vitro.Conclusions: The 2.00,4.00 and 8.00 μmol/L of luteolin can promote the proliferation,differentiation and mineralization and the expressions pf Lrp5 and β-catenin in MC3T3-E1 osteoblasts.
2017, 42(1): 48-51.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.013
Abstract:
Objective: To investigate the changes of serum amino acid levels in type 1 and type 2 diabetic rats,and its correlation between the amino acid metabolism and diabetes.Methods: The type 1 diabetes mellitus model induced by streptozotocin and type 2 spontaneous goto-kakizaki diabetic male rats were divided into the experiment group,and male Wistar rats injected by the same volume of sodium citrate suspension were set as the control group.The serum levels of 14 kinds of amino acid level were determined by liquid chromatography-mass spectrometry.Results: Compared with the control group,the levels of valine and isoleucine in experiment group increased(P<0.05 to P<0.01),and the levels of glutamine,lysine,arginine and tyrosine in experiment group decreased(P<0.05 to P<0.01).Conclusions: The levels of branched-chain amino acid and basic amino acid in type 2 diabetic mode increase and decrease,respectively.The change of amino acid level can provide some clues and help in the occurrence of complications.
Objective: To investigate the changes of serum amino acid levels in type 1 and type 2 diabetic rats,and its correlation between the amino acid metabolism and diabetes.Methods: The type 1 diabetes mellitus model induced by streptozotocin and type 2 spontaneous goto-kakizaki diabetic male rats were divided into the experiment group,and male Wistar rats injected by the same volume of sodium citrate suspension were set as the control group.The serum levels of 14 kinds of amino acid level were determined by liquid chromatography-mass spectrometry.Results: Compared with the control group,the levels of valine and isoleucine in experiment group increased(P<0.05 to P<0.01),and the levels of glutamine,lysine,arginine and tyrosine in experiment group decreased(P<0.05 to P<0.01).Conclusions: The levels of branched-chain amino acid and basic amino acid in type 2 diabetic mode increase and decrease,respectively.The change of amino acid level can provide some clues and help in the occurrence of complications.
2017, 42(1): 52-56.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.014
Abstract:
Objective: To evaluate the clinical effects of Dachengqi decoction combined with Western medicine in the treatment of early postoperative inflammatory intestinal obstruction.Methods: The related literatures were searched from CNKI,CBM and Wanfang database,and statistically analyzed using RevMan5.2 software.Results: Twenty-one studies and 1 037 patients were selected.The results of Meta-analysis showed that the cure rate of the Dachengqi decoction combined with Western medicine in the treatment of early postoperative inflammatory intestinal obstruction was higher than that of Western medicine[OR=5.16,95%CI(3.30 to 8.06),P<0.01].The recovery time of anus exhaust in the patients treated with the Dachengqi decoction combined with Western medicine was shorter than that in the patients treated with Western medicine[WMD=-1.50,95%CI(-1.95 to -1.15),P<0.01].The hospitalization time in the patients treated with the Dachengqi decoction combined with Western medicine was shorter than that in the patients treated with Western medicine[WMD=-3.45,95%CI(-4.55 to -2.35),P<0.01].The serum level of interleukin-6 in the patients treated with the Dachengqi decoction combined with Western medicine was lower than that in the patients treated with Western medicine[SMD=-6.01,95%CI(-9.19 to 2.84),P<0.01].The difference of the serum level of tumor necrosis factor-α between two groups was not statistically significant[SMD=-1.87,95%CI(-4.00 to 0.27),P>0.05].Conclusions: The treatment of early postoperative inflammatory intestinal obstruction with Dachengqi decoction combined with Western medicine can effectively improve the cure rate,shorten the recovery time of of anus exhaust and hospitalization time,and decrease the serum level of interleukin-6 of patients.
Objective: To evaluate the clinical effects of Dachengqi decoction combined with Western medicine in the treatment of early postoperative inflammatory intestinal obstruction.Methods: The related literatures were searched from CNKI,CBM and Wanfang database,and statistically analyzed using RevMan5.2 software.Results: Twenty-one studies and 1 037 patients were selected.The results of Meta-analysis showed that the cure rate of the Dachengqi decoction combined with Western medicine in the treatment of early postoperative inflammatory intestinal obstruction was higher than that of Western medicine[OR=5.16,95%CI(3.30 to 8.06),P<0.01].The recovery time of anus exhaust in the patients treated with the Dachengqi decoction combined with Western medicine was shorter than that in the patients treated with Western medicine[WMD=-1.50,95%CI(-1.95 to -1.15),P<0.01].The hospitalization time in the patients treated with the Dachengqi decoction combined with Western medicine was shorter than that in the patients treated with Western medicine[WMD=-3.45,95%CI(-4.55 to -2.35),P<0.01].The serum level of interleukin-6 in the patients treated with the Dachengqi decoction combined with Western medicine was lower than that in the patients treated with Western medicine[SMD=-6.01,95%CI(-9.19 to 2.84),P<0.01].The difference of the serum level of tumor necrosis factor-α between two groups was not statistically significant[SMD=-1.87,95%CI(-4.00 to 0.27),P>0.05].Conclusions: The treatment of early postoperative inflammatory intestinal obstruction with Dachengqi decoction combined with Western medicine can effectively improve the cure rate,shorten the recovery time of of anus exhaust and hospitalization time,and decrease the serum level of interleukin-6 of patients.
2017, 42(1): 57-60,63.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.015
Abstract:
Objective: To study the effects of dexmedetomidine(Dex) on early postoperative cognitive function in elderly patients treated with lung cancer operation,and its correlation with the anesthetic drug dose.Methods: Sixty elderly patients scheduled by lobectomy or pneumonectomy under general anesthesia were randomly divided into the control group(group C),low dose Dex group(group LD),middle dose Dex group(group MD) and high dose Dex group(group HD)(15 cases each group).The mean arterial pressure,heart rate and blood oxygen saturation in all patients were detected before anesthesia induction(T0),opening the chest(T1),end of operation(T2) and 1 d after operation LD(T3).The postoperative cognitive function in four groups were measured using mini-mental state examination method(MMSE) 1 d before operation,and 1 d,3 d and 7 d after operation.The sedation scores of all patients were recorded using the Sedation-Agitation Scale in postanesthesia care unit(PACU).Results: Compared with the T0 time-point,the mean arterial pressure at T1 in group C significantly increased(P<0.01),and the heart rate at T2 and T3 in LD,MD and HD groups decreased(P<0.05 to P<0.01).Compared with the group C,the heart rate at T1 to T3 decreased(P<0.05 to P<0.01).The differences of the PACU scores between four groups were not statistically significant(P>0.05).Compared 1 d before operation,the MMSE score in group C decreased significantly 1 d after operation(P<0.01).Compared with group C,the MMSE scores in other three groups significantly increased 1 d after operation(P<0.01).The differences of the incidences of postoperative cognitive dysfunction between four groups were statistically significant 1 d after operation(P<0.05).Conclusions: The application of Dex in elderly patients treated with lobectomy or pneumonectomy can maintan the hemodynamic stability,and reduce the incidence of postoperative cognitive dysfunction,which has no obvious dependence on the anesthetic drug dose.
Objective: To study the effects of dexmedetomidine(Dex) on early postoperative cognitive function in elderly patients treated with lung cancer operation,and its correlation with the anesthetic drug dose.Methods: Sixty elderly patients scheduled by lobectomy or pneumonectomy under general anesthesia were randomly divided into the control group(group C),low dose Dex group(group LD),middle dose Dex group(group MD) and high dose Dex group(group HD)(15 cases each group).The mean arterial pressure,heart rate and blood oxygen saturation in all patients were detected before anesthesia induction(T0),opening the chest(T1),end of operation(T2) and 1 d after operation LD(T3).The postoperative cognitive function in four groups were measured using mini-mental state examination method(MMSE) 1 d before operation,and 1 d,3 d and 7 d after operation.The sedation scores of all patients were recorded using the Sedation-Agitation Scale in postanesthesia care unit(PACU).Results: Compared with the T0 time-point,the mean arterial pressure at T1 in group C significantly increased(P<0.01),and the heart rate at T2 and T3 in LD,MD and HD groups decreased(P<0.05 to P<0.01).Compared with the group C,the heart rate at T1 to T3 decreased(P<0.05 to P<0.01).The differences of the PACU scores between four groups were not statistically significant(P>0.05).Compared 1 d before operation,the MMSE score in group C decreased significantly 1 d after operation(P<0.01).Compared with group C,the MMSE scores in other three groups significantly increased 1 d after operation(P<0.01).The differences of the incidences of postoperative cognitive dysfunction between four groups were statistically significant 1 d after operation(P<0.05).Conclusions: The application of Dex in elderly patients treated with lobectomy or pneumonectomy can maintan the hemodynamic stability,and reduce the incidence of postoperative cognitive dysfunction,which has no obvious dependence on the anesthetic drug dose.
2017, 42(1): 61-63.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.016
Abstract:
Objective: To investigate the influencing factors of the slow healing of perineal wound after Miles operation of rectal cancer.Methods: The clinical data of 210 rectal cancer patients diagnosed by pathology were retrospectively analyzed after Miles operation.The influencing factors of the slow healing of perineal wound,which included the gender,age,preoperative nutritional status,intraoperative pelvic peritoneal repair or not,preoperative radiotherapy or not and preoperative and postoperative hyperbaric oxygen therapy,were investigated,and the correlation of the poor healing of incision with influencing factor were statistically analyzed.Results: The difference of the primary healing rate of incision in patients with different gender,age,preoperative nutritional status and peritoneal repair were not statistically significant(P>0.05).The difference of the primary healing rate of incision in patients with preoperative radiotherapy was significant lower than that in patients without preoperative radiotherapy(P<0.01),and the difference of the primary healing rate of incision in patients with preoperative and postoperative hyperbaric oxygen therapy was significant higher than that in patients without preoperative and postoperative hyperbaric oxygen therapy(P<0.05).Conclusions: After Miles operation of rectal cancer,the preoperative radiotherapy can delay the primary healing of incision,the preoperative and postoperative hyperbaric oxygen therapy can promote the primary healing of incision,and the hyperbaric oxygen therapy can be used to prevent the poor wound healing.
Objective: To investigate the influencing factors of the slow healing of perineal wound after Miles operation of rectal cancer.Methods: The clinical data of 210 rectal cancer patients diagnosed by pathology were retrospectively analyzed after Miles operation.The influencing factors of the slow healing of perineal wound,which included the gender,age,preoperative nutritional status,intraoperative pelvic peritoneal repair or not,preoperative radiotherapy or not and preoperative and postoperative hyperbaric oxygen therapy,were investigated,and the correlation of the poor healing of incision with influencing factor were statistically analyzed.Results: The difference of the primary healing rate of incision in patients with different gender,age,preoperative nutritional status and peritoneal repair were not statistically significant(P>0.05).The difference of the primary healing rate of incision in patients with preoperative radiotherapy was significant lower than that in patients without preoperative radiotherapy(P<0.01),and the difference of the primary healing rate of incision in patients with preoperative and postoperative hyperbaric oxygen therapy was significant higher than that in patients without preoperative and postoperative hyperbaric oxygen therapy(P<0.05).Conclusions: After Miles operation of rectal cancer,the preoperative radiotherapy can delay the primary healing of incision,the preoperative and postoperative hyperbaric oxygen therapy can promote the primary healing of incision,and the hyperbaric oxygen therapy can be used to prevent the poor wound healing.
Analysis of positive results and drug resistance in 76 cases of blood culture of neonatal septicemia
2017, 42(1): 64-66.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.017
Abstract:
Objective: To understand the neonatal septicemia pathogen and drug resistance analysis of pathogens,and provide the basis for clinical rational use of antibiotics,timely and clearly.Methods: Detections of pathogenic bacteria and drug sensitivity results were retrospectively analyzed in neonatal septicemia.Results: Seventy-six strains of pathogens were isolated in the blood samples in clinical diagnosis of sepsis,including 71 strains of gram positive bacteria cases(93.4%) The top three were Staphylococcus epidermidis 35(46.0%),Staphylococcus haemolyticus 20(26.3%),Enterococcus 6 cases(7.9%);4 strains of gram negative bacteria(5.3%);Candida 1 strains(1.3%) of gram positive bacteria.The resistance that gram positive bacteria to penicillin,amoxicillin and clavulanate potassium and ampicillin,oxacillin was high,There was no resistance to vancomycin and linezolid.Conclusions: It is important to detect the drug resistance of pathogenic bacteria of neonatal septicemia,and base on the drug sensitivity results of sensitive antimicrobial drugs to take rational use of antibiotics to reduce the emergence of bacterial resistance,improve the clinical treatment effect.
Objective: To understand the neonatal septicemia pathogen and drug resistance analysis of pathogens,and provide the basis for clinical rational use of antibiotics,timely and clearly.Methods: Detections of pathogenic bacteria and drug sensitivity results were retrospectively analyzed in neonatal septicemia.Results: Seventy-six strains of pathogens were isolated in the blood samples in clinical diagnosis of sepsis,including 71 strains of gram positive bacteria cases(93.4%) The top three were Staphylococcus epidermidis 35(46.0%),Staphylococcus haemolyticus 20(26.3%),Enterococcus 6 cases(7.9%);4 strains of gram negative bacteria(5.3%);Candida 1 strains(1.3%) of gram positive bacteria.The resistance that gram positive bacteria to penicillin,amoxicillin and clavulanate potassium and ampicillin,oxacillin was high,There was no resistance to vancomycin and linezolid.Conclusions: It is important to detect the drug resistance of pathogenic bacteria of neonatal septicemia,and base on the drug sensitivity results of sensitive antimicrobial drugs to take rational use of antibiotics to reduce the emergence of bacterial resistance,improve the clinical treatment effect.
2017, 42(1): 67-70.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.018
Abstract:
Objective: To investigate the clinical value of the levels of urine neutrophil gelatinase-associated lipocalin(NGAL) and interleukin-18(IL-18) in diabetic nephropathy(DN).Methods: According to the urinary albumin/urine creatinine(ACR),98 patients with type 2 diabetes mellitus were divided into the normal albuminuria group(ACR less than 30 mg/g),micro albuminuria group(ACR ranging from 30 to 300 mg/g) and macro albuminuria group(ACR more than 300 mg/g).Thirty healthy people were set as the control group.The levels of urine NGAL and IL-18 in 4 groups were detected by ELISA,the correlations of which with ACR and glomerular filtration rate(GFR) were analyzed.Results: The levels of urine NGAL and IL-18 in 4 groups increased with the increasing of ACR level(P<0.01).The levels of urine NGAL and IL-18 were positive correlation with the ACR(P<0.01),and negative correlation with the GFR(P<0.01).The level of urine NGAL was positive correlation with the urine IL-18(P<0.01).Conclusions: The levels of urine NGAL and IL-18 increase obviously in DN patients.In addition to the glomerular damage,the renal tubular damage still exists in DN patients,and the renal tubule damage is closely related to the glomerular damage.
Objective: To investigate the clinical value of the levels of urine neutrophil gelatinase-associated lipocalin(NGAL) and interleukin-18(IL-18) in diabetic nephropathy(DN).Methods: According to the urinary albumin/urine creatinine(ACR),98 patients with type 2 diabetes mellitus were divided into the normal albuminuria group(ACR less than 30 mg/g),micro albuminuria group(ACR ranging from 30 to 300 mg/g) and macro albuminuria group(ACR more than 300 mg/g).Thirty healthy people were set as the control group.The levels of urine NGAL and IL-18 in 4 groups were detected by ELISA,the correlations of which with ACR and glomerular filtration rate(GFR) were analyzed.Results: The levels of urine NGAL and IL-18 in 4 groups increased with the increasing of ACR level(P<0.01).The levels of urine NGAL and IL-18 were positive correlation with the ACR(P<0.01),and negative correlation with the GFR(P<0.01).The level of urine NGAL was positive correlation with the urine IL-18(P<0.01).Conclusions: The levels of urine NGAL and IL-18 increase obviously in DN patients.In addition to the glomerular damage,the renal tubular damage still exists in DN patients,and the renal tubule damage is closely related to the glomerular damage.
2017, 42(1): 70-72.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.019
Abstract:
Objective: To investigate the tuberculosis(TB) infection risks of medical personnel and its intervention strategy in TB hospital.Methods: The age,gender,length of work,type of personnel,marital status,title and education level in 86 medical personnel were investigated using self-designed questionnaire,which was evaluated using Likert 5-rating method.The influencing factors of TB infection risk were analyzed,and the corresponding intervention strategies were formulated.Results: The age,length of work,type of personnel and professional title were the main risks of TB infection of medical personnel in TB hospital(P<0.01).Conclusions: In the process of clinical medical care in TB hospital,the medical personnel face many infection risks.The corresponding intervention strategy should be formulated by medical institution according to the individual difference,which is used to safeguard the health of medical personnel.
Objective: To investigate the tuberculosis(TB) infection risks of medical personnel and its intervention strategy in TB hospital.Methods: The age,gender,length of work,type of personnel,marital status,title and education level in 86 medical personnel were investigated using self-designed questionnaire,which was evaluated using Likert 5-rating method.The influencing factors of TB infection risk were analyzed,and the corresponding intervention strategies were formulated.Results: The age,length of work,type of personnel and professional title were the main risks of TB infection of medical personnel in TB hospital(P<0.01).Conclusions: In the process of clinical medical care in TB hospital,the medical personnel face many infection risks.The corresponding intervention strategy should be formulated by medical institution according to the individual difference,which is used to safeguard the health of medical personnel.
2017, 42(1): 73-75.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.020
Abstract:
Objective: To investigate the effects of telbivudine and lamivudine in the treatment of chronic hepatitis B(CHB).Methods: Eighty-two CHB patients were randomly divided into the telbivudine group and lamivudine group(41 cases each group).Telbivudine group and lamivudine group were treated with telbivudine and lamivudine,respectively.The levels of HBV-DNA,HBeAg,AST and ALT in two groups between before and after treatment were compared.The rates of negative-inverted of HBV-DNA and HBeAg and recovery rates of AST and ALT in two groups were compared after 12,24 and 48 weeks of treatment,the safety of which was evaluated.Results: After 12,24 and 48 weeks of treatment,the levels of HBV-DNA,HBeAg,AST and ALT in two groups were significantly lower than before treatment(P<0.01).After 48 weeks of treatment,the rate of negative-inverted of HBV-DNA in telbivudine group was higher than that in lamivudine group(P<0.05).After 24 and 48 weeks of treatment,the recovery rates of AST and ALT in telbivudine group were significantly higher than those in lamivudine group(P<0.05).Conclusions: The treatment of CHB patients with telbivudine is good effect and safety,which is worthy of clinical use.
Objective: To investigate the effects of telbivudine and lamivudine in the treatment of chronic hepatitis B(CHB).Methods: Eighty-two CHB patients were randomly divided into the telbivudine group and lamivudine group(41 cases each group).Telbivudine group and lamivudine group were treated with telbivudine and lamivudine,respectively.The levels of HBV-DNA,HBeAg,AST and ALT in two groups between before and after treatment were compared.The rates of negative-inverted of HBV-DNA and HBeAg and recovery rates of AST and ALT in two groups were compared after 12,24 and 48 weeks of treatment,the safety of which was evaluated.Results: After 12,24 and 48 weeks of treatment,the levels of HBV-DNA,HBeAg,AST and ALT in two groups were significantly lower than before treatment(P<0.01).After 48 weeks of treatment,the rate of negative-inverted of HBV-DNA in telbivudine group was higher than that in lamivudine group(P<0.05).After 24 and 48 weeks of treatment,the recovery rates of AST and ALT in telbivudine group were significantly higher than those in lamivudine group(P<0.05).Conclusions: The treatment of CHB patients with telbivudine is good effect and safety,which is worthy of clinical use.
2017, 42(1): 76-78.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.021
Abstract:
Objective: To explore the effects of different anesthesia methods on the postoperative pulmonary infection in elderly patients with esophageal carcinoma.Methods: Eighty patients with esophageal cancer were divided into the control group and observation group according to the anesthesia method(40 cases each group).The control group and observation group were anesthetized using single lumen endotracheal intubation and single lumen endotracheal intubation combined with epidural,respectively.The amount of bleeding,operation time,postoperative 2 d visual analogue score,hospitalization time,recovery time,extubation time and postoperative pulmonary infection in two groups were observed.Results: Compared with the control group,the recovery time and extubation time in the observation group significantly shortened(P<0.01),and the incidence of pulmonary infection in observation group was significantly less than that in control group(P<0.05).Conclusions: General anesthesia combined with epidural anesthesia is suit for elderly patients with esophageal cancer,the anesthesia effect of which is ideal.The incidence of pulmonary infection after general anesthesia with endotracheal intubation is lower,which is worthy of clinical application.
Objective: To explore the effects of different anesthesia methods on the postoperative pulmonary infection in elderly patients with esophageal carcinoma.Methods: Eighty patients with esophageal cancer were divided into the control group and observation group according to the anesthesia method(40 cases each group).The control group and observation group were anesthetized using single lumen endotracheal intubation and single lumen endotracheal intubation combined with epidural,respectively.The amount of bleeding,operation time,postoperative 2 d visual analogue score,hospitalization time,recovery time,extubation time and postoperative pulmonary infection in two groups were observed.Results: Compared with the control group,the recovery time and extubation time in the observation group significantly shortened(P<0.01),and the incidence of pulmonary infection in observation group was significantly less than that in control group(P<0.05).Conclusions: General anesthesia combined with epidural anesthesia is suit for elderly patients with esophageal cancer,the anesthesia effect of which is ideal.The incidence of pulmonary infection after general anesthesia with endotracheal intubation is lower,which is worthy of clinical application.
2017, 42(1): 78-80.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.022
Abstract:
Objective: To investigate the safety and clinical effects of the pereutaneous nephrolithotomy(PCNL) guided by ordinary ultrasound.Methods: One hundred and sixty patients treated with pereutaneous nephrolithotomy guided by ordinary ultrasound.The success puncture rate in percutaneous renal channel,bleeding and postoperative complications were retrospectively analyzed.Results: Total 198 channels were established in 169 cases.The success rate of puncture was 96.0%(190/198).The second puncture operation for looking for the residual stone was not successful,but the puncture combined with C-arm X-ray was successful.Seven patients with postoperative blood transfusion for 200 mL to 400 mL were treated with non-selective renal artery embolization.The pleural effusion in 18 cases were identified,and 1 case need to be closed drainage.The postoperative fever in 11 cases,no infectious shock,no poor prognosis,and lungs and other visceral injury were found.Conclusions: The improved percutaneous renal punture guided by ordinary ultrasound in pereutaneous nephrolithotomy is simple,rapid,accurate,economic,high successful rate,which can effectively reduce the difficulty of puncture,and is worthy of promotion in primary hospitals.
Objective: To investigate the safety and clinical effects of the pereutaneous nephrolithotomy(PCNL) guided by ordinary ultrasound.Methods: One hundred and sixty patients treated with pereutaneous nephrolithotomy guided by ordinary ultrasound.The success puncture rate in percutaneous renal channel,bleeding and postoperative complications were retrospectively analyzed.Results: Total 198 channels were established in 169 cases.The success rate of puncture was 96.0%(190/198).The second puncture operation for looking for the residual stone was not successful,but the puncture combined with C-arm X-ray was successful.Seven patients with postoperative blood transfusion for 200 mL to 400 mL were treated with non-selective renal artery embolization.The pleural effusion in 18 cases were identified,and 1 case need to be closed drainage.The postoperative fever in 11 cases,no infectious shock,no poor prognosis,and lungs and other visceral injury were found.Conclusions: The improved percutaneous renal punture guided by ordinary ultrasound in pereutaneous nephrolithotomy is simple,rapid,accurate,economic,high successful rate,which can effectively reduce the difficulty of puncture,and is worthy of promotion in primary hospitals.
2017, 42(1): 81-83.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.023
Abstract:
Objective: To explore the application value of casual blood pressure and 24-hour ambulatory blood pressure monitoring in the diagnosis of pregnancy-induced hypertension disease.Methods: One hundred and twenty patients with pregnancy-induced hypertension were divided into the primipara group(90 cases) and multipara group(30 cases).The casual blood pressure and 24-hour ambulatory blood pressure monitoring in two groups were implemented,and the results of which were analyzed.Results: The difference of diastolic blood pressure(DBP) between two groups was not statistically significant(P>0.05).The DBP of the casual blood pressure and 24-hour ambulatory blood pressure in primipara group were higher than those in multipara group(P<0.05 to P<0.01).The diagnosis rate of pregnancy-induced hypertension disease of casual blood pressure(100.0%) was significantly higher than that of 24-hour ambulatory blood pressure(25.0%)(P<0.01).The detection rate of pregnancy-induced hypertension disease of 24-hour ambulatory blood pressure in primipara group(12.22%) was significantly lower than that in multipara group(63.33%)(P<0.01).Conclusions: Compared with the casual blood pressure,the 24-hour ambulatory blood pressure monitoring can continuously record the change of 24h blood pressure,which is convenient,can really reflect the blood pressure levels of the whole day,and has some advantages in the diagnosis and screening of pregnancy-induced hypertension disease.
Objective: To explore the application value of casual blood pressure and 24-hour ambulatory blood pressure monitoring in the diagnosis of pregnancy-induced hypertension disease.Methods: One hundred and twenty patients with pregnancy-induced hypertension were divided into the primipara group(90 cases) and multipara group(30 cases).The casual blood pressure and 24-hour ambulatory blood pressure monitoring in two groups were implemented,and the results of which were analyzed.Results: The difference of diastolic blood pressure(DBP) between two groups was not statistically significant(P>0.05).The DBP of the casual blood pressure and 24-hour ambulatory blood pressure in primipara group were higher than those in multipara group(P<0.05 to P<0.01).The diagnosis rate of pregnancy-induced hypertension disease of casual blood pressure(100.0%) was significantly higher than that of 24-hour ambulatory blood pressure(25.0%)(P<0.01).The detection rate of pregnancy-induced hypertension disease of 24-hour ambulatory blood pressure in primipara group(12.22%) was significantly lower than that in multipara group(63.33%)(P<0.01).Conclusions: Compared with the casual blood pressure,the 24-hour ambulatory blood pressure monitoring can continuously record the change of 24h blood pressure,which is convenient,can really reflect the blood pressure levels of the whole day,and has some advantages in the diagnosis and screening of pregnancy-induced hypertension disease.
2017, 42(1): 97-98,101.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.030
Abstract:
Objective: To explore the clinical value of high frequency color ultrasonography in the diagnosis of phyllodes tumor of the breast(PTB),and improve the understanding of the disease.Methods: Twenty small volume malignant PTB female patients diagnosed by pathology were examined using high frequency color ultrasonography.Results: Among 20 PTB patients,5 cases tumors were lobular,14 cases tumors were oval,and 1 case tumor was irregular.The boundary of tumor in 18 cases were clear,and 2 cases were unclear.The attenuation,unchanged and enhancement of the internal echo of tumor were 0 case,5 cases and 15 cases,respectively.The cystic change in 6 cases and calcification in 2 cases were found.The results of color Doppler flow imaging showed that the blood flow signals in all patients could be detected,which included class Ⅰ in 9 cases,class Ⅱ in 7 cases and class Ⅲ in 4 cases.The average of the systolic peak velocity and resistance index of blood flow in 20 cases were (19.35±6.50) cm and (0.72±0.04),respectively.The pathological types of 20 cases included benign tumor in 9 cases,borderline tumor in 5 cases and malignant in 6 cases.There was not significant corresponding relationship between the blood flow signal class and PTB pathological type.Conclusions: The high frequency color ultrasonography can clearly display the characteristic sonogram of the small volume malignant PTB,which can provide the high clinical value in the differential diagnosis and selecting appropriate clinical treatment program for tumor.
Objective: To explore the clinical value of high frequency color ultrasonography in the diagnosis of phyllodes tumor of the breast(PTB),and improve the understanding of the disease.Methods: Twenty small volume malignant PTB female patients diagnosed by pathology were examined using high frequency color ultrasonography.Results: Among 20 PTB patients,5 cases tumors were lobular,14 cases tumors were oval,and 1 case tumor was irregular.The boundary of tumor in 18 cases were clear,and 2 cases were unclear.The attenuation,unchanged and enhancement of the internal echo of tumor were 0 case,5 cases and 15 cases,respectively.The cystic change in 6 cases and calcification in 2 cases were found.The results of color Doppler flow imaging showed that the blood flow signals in all patients could be detected,which included class Ⅰ in 9 cases,class Ⅱ in 7 cases and class Ⅲ in 4 cases.The average of the systolic peak velocity and resistance index of blood flow in 20 cases were (19.35±6.50) cm and (0.72±0.04),respectively.The pathological types of 20 cases included benign tumor in 9 cases,borderline tumor in 5 cases and malignant in 6 cases.There was not significant corresponding relationship between the blood flow signal class and PTB pathological type.Conclusions: The high frequency color ultrasonography can clearly display the characteristic sonogram of the small volume malignant PTB,which can provide the high clinical value in the differential diagnosis and selecting appropriate clinical treatment program for tumor.
2017, 42(1): 99-101.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.031
Abstract:
Objective: To explore the application value of 64-slice spiral CT 3D reconstruction technique in the nasal dorsum narrow osteotomy.Methods: Twenty nasolacrimal duct injury patients and 20 non-nasolacrimal duct injury treated with the nasal dorsum narrow osteotomy were divided into the observation group and control group.The nasolacrimal duct length,nasolacrimal duct wall thickness,nose angle,projection angle between nasolacrimal tube axial and sagittal section,angle between nasal length axis and horizontal plane and angle between nasolacrimal tube axial and coronal plane in two groups were detected using the 64-slice spiral CT 3D the 64-slice spiral CT 3D reconstruction,and analyzed.Results: The detection of 64-slice spiral CT 3D reconstruction showed that nasolacrimal duct length,nasolacrimal duct wall thickness,nose angle,projection angle between nasolacrimal tube axial and sagittal section,angle between nasal length axis and horizontal plane and angle between nasolacrimal tube axial and coronal plane in observation group were(11.58±11.58) mm,(1.25±0.36) mm,(128.51±5.65)°,(8.63±0.38)°,(73.81±3.26)° and(12.25±0.69)°,respectively,which was significantly higher than those in control group(P<0.01).Conclusions: The preoperative measurement of nasolacrimal duct with 64-slice spiral CT 3D reconstruction technique can accurately guide the nasal dorsum osteotomy,which has important clinical significance.
Objective: To explore the application value of 64-slice spiral CT 3D reconstruction technique in the nasal dorsum narrow osteotomy.Methods: Twenty nasolacrimal duct injury patients and 20 non-nasolacrimal duct injury treated with the nasal dorsum narrow osteotomy were divided into the observation group and control group.The nasolacrimal duct length,nasolacrimal duct wall thickness,nose angle,projection angle between nasolacrimal tube axial and sagittal section,angle between nasal length axis and horizontal plane and angle between nasolacrimal tube axial and coronal plane in two groups were detected using the 64-slice spiral CT 3D the 64-slice spiral CT 3D reconstruction,and analyzed.Results: The detection of 64-slice spiral CT 3D reconstruction showed that nasolacrimal duct length,nasolacrimal duct wall thickness,nose angle,projection angle between nasolacrimal tube axial and sagittal section,angle between nasal length axis and horizontal plane and angle between nasolacrimal tube axial and coronal plane in observation group were(11.58±11.58) mm,(1.25±0.36) mm,(128.51±5.65)°,(8.63±0.38)°,(73.81±3.26)° and(12.25±0.69)°,respectively,which was significantly higher than those in control group(P<0.01).Conclusions: The preoperative measurement of nasolacrimal duct with 64-slice spiral CT 3D reconstruction technique can accurately guide the nasal dorsum osteotomy,which has important clinical significance.
2017, 42(1): 104-105,110.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.033
Abstract:
Objective: To investigate the clinical value of the serum(1,3)-β-D-glucan assay in the diagnosis of invasive fungal infection.Methods: The serum levels of(1,3)-β-D-glucan in 1 100 patients were retrospectively analyzed.Results: The sensitivity,specificity,positive predictive value and negative predictive value of the(1,3)-β-D-glucan assay were 78.0%,89.9%,74.8% and 91.4%,respectively,the difference between the result of fungal G test and clinical diagnosis was statistically significant(χ2=491.25,P<0.01).The sensitivity,specificity,positive predictive value and negative predictive value of fungal culture were 48.9%,92.3%,71.0% and 82.5%,respectively,the difference between the result of fungal G test and clinical diagnosis was statistically significant(χ2=239.34,P<0.01).Conclusions: The(1,3)-β-D-glucan assay is rapid and high negative predictive value.The conventional fungal culture is long time consuming,high specificity and susceptibility to contamination.The fungal G test combined with fungal culture can improve the sensitivity and specificity of the clinical diagnosis in suspect invasive fungal infection.
Objective: To investigate the clinical value of the serum(1,3)-β-D-glucan assay in the diagnosis of invasive fungal infection.Methods: The serum levels of(1,3)-β-D-glucan in 1 100 patients were retrospectively analyzed.Results: The sensitivity,specificity,positive predictive value and negative predictive value of the(1,3)-β-D-glucan assay were 78.0%,89.9%,74.8% and 91.4%,respectively,the difference between the result of fungal G test and clinical diagnosis was statistically significant(χ2=491.25,P<0.01).The sensitivity,specificity,positive predictive value and negative predictive value of fungal culture were 48.9%,92.3%,71.0% and 82.5%,respectively,the difference between the result of fungal G test and clinical diagnosis was statistically significant(χ2=239.34,P<0.01).Conclusions: The(1,3)-β-D-glucan assay is rapid and high negative predictive value.The conventional fungal culture is long time consuming,high specificity and susceptibility to contamination.The fungal G test combined with fungal culture can improve the sensitivity and specificity of the clinical diagnosis in suspect invasive fungal infection.
Analysis of the sleep quality and its relative impact factors in a medical academy students of Hebei
2017, 42(1): 106-110.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.034
Abstract:
Objective: To investigate the sleep status and its impact factors in a medical academy students.Methods: The medical academy students were investigated using the Pittsburgh Sleep Quality Index(PSQI) and self-made sleep impact factors questionnaire by stratified random sampling surveys.Results: The total score of students PSQI was(8.13±2.65),the detection rate of sleep problem was 54.85%.The scores of the sleep time and sleep disorders in boy students were lower than that in girl students(P<0.05 and P<0.01),and the score of the length of sleep time in boy students was higher than that in girl students(P<0.01).The differences of the score of each item of sleep quality between urban and rural students were not statistically significant(P>0.05).The scores of the sleep quality and sleep medication in medical specialty students were higher than that in nursing specialty students(P<0.05 and P<0.01).The scores of academic pressure,friendship(including classmate relation),mobile internet dependency and physical illness in boy students were lower than that in girl students(P<0.05 to P<0.01),but the score of physical training in boy students was significantly higher than that in girl students(P<0.01).The score of the economic pressure in rural student was higher than that in urban student(P<0.05),the difference of the score of other impact factors of sleep quality from different area students was not statistically significant(P>0.05).The scores of the sleep quality of student parents,specialty pressure,academic pressure and physical training in medical specialty students were significantly higher than those in nursing specialty students(P<0.01),but the scores of mobile internet dependency,hunger and physical illness in medical specialty students were significantly lower than those in nursing specialty students(P<0.01),and sleep medication in medical specialty students were higher than that in nursing specialty students(P<0.01).The total score of the impact factors was positively correlated with the total score of PSQI(P<0.01).Except the teacher-student relationship,the total score of other 14 factors was positively correlated with the total score of PSQI(P<0.01).The coefficient correlation of specialty was the highest(r=0.199),and the coefficient correlation of hunger was the lowest(r=0.084).Conclusions: The sleep quality and mental health education of aca demy students should be emphasized and improved,which can be achieved in life style,interpersonal relationship,school administration and psychological counseling.
Objective: To investigate the sleep status and its impact factors in a medical academy students.Methods: The medical academy students were investigated using the Pittsburgh Sleep Quality Index(PSQI) and self-made sleep impact factors questionnaire by stratified random sampling surveys.Results: The total score of students PSQI was(8.13±2.65),the detection rate of sleep problem was 54.85%.The scores of the sleep time and sleep disorders in boy students were lower than that in girl students(P<0.05 and P<0.01),and the score of the length of sleep time in boy students was higher than that in girl students(P<0.01).The differences of the score of each item of sleep quality between urban and rural students were not statistically significant(P>0.05).The scores of the sleep quality and sleep medication in medical specialty students were higher than that in nursing specialty students(P<0.05 and P<0.01).The scores of academic pressure,friendship(including classmate relation),mobile internet dependency and physical illness in boy students were lower than that in girl students(P<0.05 to P<0.01),but the score of physical training in boy students was significantly higher than that in girl students(P<0.01).The score of the economic pressure in rural student was higher than that in urban student(P<0.05),the difference of the score of other impact factors of sleep quality from different area students was not statistically significant(P>0.05).The scores of the sleep quality of student parents,specialty pressure,academic pressure and physical training in medical specialty students were significantly higher than those in nursing specialty students(P<0.01),but the scores of mobile internet dependency,hunger and physical illness in medical specialty students were significantly lower than those in nursing specialty students(P<0.01),and sleep medication in medical specialty students were higher than that in nursing specialty students(P<0.01).The total score of the impact factors was positively correlated with the total score of PSQI(P<0.01).Except the teacher-student relationship,the total score of other 14 factors was positively correlated with the total score of PSQI(P<0.01).The coefficient correlation of specialty was the highest(r=0.199),and the coefficient correlation of hunger was the lowest(r=0.084).Conclusions: The sleep quality and mental health education of aca demy students should be emphasized and improved,which can be achieved in life style,interpersonal relationship,school administration and psychological counseling.
2017, 42(1): 111-116.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.035
Abstract:
Objective: To study the prescription,preparation technology and quality standard of Kuijieling tablet.Methods: Single factor experiments were used to investigate the important factors affecting the process of tablet forming;the five herb medicine in this prescription were identified by thin layer chromatography(TLC) and atractylenolide Ⅰand atractylenolide Ⅲ were quantitatively determined by high performance liquid chromatography.Results: The formula for determining the core of Kuijieling was as follows:Disintegration agent was L-HPC and PVPP each 8%,the binder was 95% ethanol solution,and the lubricant was 0.25% magnesium stearate;spots in the TLC chromatogram for qualitative identification of the five herb medicine were clear and the resolution was high.AtractylenolideⅠshowed a good linear relationship in the range of 0.024 5-0.220 5 μg/mL and atractylenolide Ⅲ showed a good linear relationship in the range of 0.012 5-0.112 5 μg/mL.The results of the various examinations on Kuijieling colon specific tablets were all in line with the requirements of the Pharmacopoeia.Conclusions: The prescription process is scientific and reasonable.The established quality standard method is feasible.
Objective: To study the prescription,preparation technology and quality standard of Kuijieling tablet.Methods: Single factor experiments were used to investigate the important factors affecting the process of tablet forming;the five herb medicine in this prescription were identified by thin layer chromatography(TLC) and atractylenolide Ⅰand atractylenolide Ⅲ were quantitatively determined by high performance liquid chromatography.Results: The formula for determining the core of Kuijieling was as follows:Disintegration agent was L-HPC and PVPP each 8%,the binder was 95% ethanol solution,and the lubricant was 0.25% magnesium stearate;spots in the TLC chromatogram for qualitative identification of the five herb medicine were clear and the resolution was high.AtractylenolideⅠshowed a good linear relationship in the range of 0.024 5-0.220 5 μg/mL and atractylenolide Ⅲ showed a good linear relationship in the range of 0.012 5-0.112 5 μg/mL.The results of the various examinations on Kuijieling colon specific tablets were all in line with the requirements of the Pharmacopoeia.Conclusions: The prescription process is scientific and reasonable.The established quality standard method is feasible.
2017, 42(1): 121-125.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.037
Abstract:
Objective: To investigate the real psychological experience in puerperas with late pregnancy complicated with acute aortic dissection.Methods: Four puerperas with late pregnancy complicated with acute aortic dissection were interviewed using qualitative research,the data of which was analyzed by Colaizzi method.Results: The worrying about disease,self-accusation and guilt,financial depress,and social role change and requirement for social support were the psychological experiences of the puerpera with late pregnancy complicated with acute aortic dissection according to the analysis and classification of the interview.Conclusions: The puerpera with late pregnancy complicated with acute aortic dissection lacks of the relevant knowledge of dissecting aneurysm disease,and has multiple negative psychological experience during the course of ill,delivery and operation.The medical staff can provide the effective support and intervent to help the patients safely pass the special time.
Objective: To investigate the real psychological experience in puerperas with late pregnancy complicated with acute aortic dissection.Methods: Four puerperas with late pregnancy complicated with acute aortic dissection were interviewed using qualitative research,the data of which was analyzed by Colaizzi method.Results: The worrying about disease,self-accusation and guilt,financial depress,and social role change and requirement for social support were the psychological experiences of the puerpera with late pregnancy complicated with acute aortic dissection according to the analysis and classification of the interview.Conclusions: The puerpera with late pregnancy complicated with acute aortic dissection lacks of the relevant knowledge of dissecting aneurysm disease,and has multiple negative psychological experience during the course of ill,delivery and operation.The medical staff can provide the effective support and intervent to help the patients safely pass the special time.
2017, 42(1): 125-127.
doi: 10.13898/j.cnki.issn.1000-2200.2017.01.038
Abstract:
Objective: To investigate the effects of perioperative comprehensive respiratory function training on postoperative lung founction after abdominal surgery in elderly patients.Methods: Seventy-three elderly patients treated with laparoscopic appendectomy or cholecystectomy were randomly divided into the control group(36 cases) and observation group(37 cases).The control group and observation group were treated with the routine nursing and respiratory function training,respectively.The postoperative complication,preoperative and postoperative lung function and blood gas between two groups were compared.Results: The difference of the incidence rate of complication between two grouops was not statistically significnat(P>0.05).Compared before operation,the postoperative FEV1 and FEV1/FEV in control group were decreased(P<0.05),and the postoperative FEV1 and FEV1/FEV in observation group were improved compared with the control group(P<0.05).Compared before operation,the postoperative SaO2 and PaO2 significantly decreased,and the postoperative PaCO2 increased in control group(P<0.05).Compared with the control group,the postoperative SaO2,PaO2 and PaCO2 in observation group were improved(P<0.05).Conclusions: The perioperative comprehensive respiratory function training is an important way in improving respiratory function after laparoscopic surgery,which can help to reduce the incidence of postoperative respiratory complications.
Objective: To investigate the effects of perioperative comprehensive respiratory function training on postoperative lung founction after abdominal surgery in elderly patients.Methods: Seventy-three elderly patients treated with laparoscopic appendectomy or cholecystectomy were randomly divided into the control group(36 cases) and observation group(37 cases).The control group and observation group were treated with the routine nursing and respiratory function training,respectively.The postoperative complication,preoperative and postoperative lung function and blood gas between two groups were compared.Results: The difference of the incidence rate of complication between two grouops was not statistically significnat(P>0.05).Compared before operation,the postoperative FEV1 and FEV1/FEV in control group were decreased(P<0.05),and the postoperative FEV1 and FEV1/FEV in observation group were improved compared with the control group(P<0.05).Compared before operation,the postoperative SaO2 and PaO2 significantly decreased,and the postoperative PaCO2 increased in control group(P<0.05).Compared with the control group,the postoperative SaO2,PaO2 and PaCO2 in observation group were improved(P<0.05).Conclusions: The perioperative comprehensive respiratory function training is an important way in improving respiratory function after laparoscopic surgery,which can help to reduce the incidence of postoperative respiratory complications.
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