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2017 Vol. 42, No. 10
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2017, 42(10): 1293-1297,1301.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.001
Abstract:
Objective:To compare the effects between 5/6 nephrectomy and adenine irrigation in establishing the rat model of chronic renal failure complicated with renal anemia on the kidney function of rats.Methods:Twenty rats were randomly divided into the control group,model 1,2 and 3 groups(5 rats each group).The control group was irrigated with 2ml sterilized distilled water everyday,The model 1 was established with Platt method,the model 2 was established with 200 mg·kg-1·d-1 adenine for 2 weeks,then one time two days for 2 weeks,and the model 3 were established with 300 mg·kg-1·d-1 adenine for 10 days,then 250 mg·kg-1·d-1 adenine for 11 days.After 8 weeks of experiment,the parameters of the body mass,blood pressure,hemoglobin and renal function,the renal pathological changes,and the expressions of α-smooth muscle actin,osteopontin,and type Ⅰ and Ⅲ collagen in kidney tissue detected by immunohistochemical method between two groups were compared.Results:The body mass in model 2 and 3 groups was significantly lower than that in control group(P<0.01).The blood pressure in four groups increased in varying degrees(P<0.05 to P<0.01).Compared with the control group,the levels of hemoglobin in three model groups significnatly decreased(P<0.01),the levels of serum creatinine and urea nitrogen in model groups increased(P<0.05 to P<0.01),and the level of of uric acid in model 3 group increased (P<0.05).The renal morphology and color changes in model groups were found.Compared with control group,the levels of α-smooth muscle actin,osteopontin,and type Ⅰ and Ⅲ collagen in three model groups significnatly increased(P<0.05 to P<0.01).Conclusions:Both 5/6 nephrectomy and adenine can be used to establish the rat model of chronic renal failure complicated with renal anemia,and the appropriate method and drug dose should be chosen according to the purpose of the experiment.
Objective:To compare the effects between 5/6 nephrectomy and adenine irrigation in establishing the rat model of chronic renal failure complicated with renal anemia on the kidney function of rats.Methods:Twenty rats were randomly divided into the control group,model 1,2 and 3 groups(5 rats each group).The control group was irrigated with 2ml sterilized distilled water everyday,The model 1 was established with Platt method,the model 2 was established with 200 mg·kg-1·d-1 adenine for 2 weeks,then one time two days for 2 weeks,and the model 3 were established with 300 mg·kg-1·d-1 adenine for 10 days,then 250 mg·kg-1·d-1 adenine for 11 days.After 8 weeks of experiment,the parameters of the body mass,blood pressure,hemoglobin and renal function,the renal pathological changes,and the expressions of α-smooth muscle actin,osteopontin,and type Ⅰ and Ⅲ collagen in kidney tissue detected by immunohistochemical method between two groups were compared.Results:The body mass in model 2 and 3 groups was significantly lower than that in control group(P<0.01).The blood pressure in four groups increased in varying degrees(P<0.05 to P<0.01).Compared with the control group,the levels of hemoglobin in three model groups significnatly decreased(P<0.01),the levels of serum creatinine and urea nitrogen in model groups increased(P<0.05 to P<0.01),and the level of of uric acid in model 3 group increased (P<0.05).The renal morphology and color changes in model groups were found.Compared with control group,the levels of α-smooth muscle actin,osteopontin,and type Ⅰ and Ⅲ collagen in three model groups significnatly increased(P<0.05 to P<0.01).Conclusions:Both 5/6 nephrectomy and adenine can be used to establish the rat model of chronic renal failure complicated with renal anemia,and the appropriate method and drug dose should be chosen according to the purpose of the experiment.
2017, 42(10): 1298-1301.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.002
Abstract:
Objective:To explore the expressions and correlations of G-protein coupled receptor 30(GPR30),estrogen receptor α(ERα) and cyclin D1 in papillary thyroid carcinoma(PTC) tissues.Methods:The expression levels of GPR30,ERα and cyclin D1 in 76 PTC samples were detected using immunohistochemical S-P method.The differences of the positive expression rates of GPR30,ERα and cyclin D1,and correlations of GPR30,ERα and cyclin D1 with pathological features were analyzed.Results:The positive expression rates of GPR30,ERα and cyclin D1 in PTC tissues were 67.1%,76.3% and 78.9%,respectively,and the expressions of GPR30,ERα and cyclin D1 in normal thyroid tissues around cancer were not found.The positive expression rates of GPR30 and cyclin D1 in patients with lymph node metastasis were higher than those in patients without metastasis(P<0.05 and P<0.01),which was not related to the age and gender of patients,and size and MACIS score of tumor.The expression rate of ERα in female patients was higher than that in male(P<0.01),and which in patients with tumor diameter ≥ 2 cm or <2 cm was not related to the age of patients,lymph node metastasis and MACIS score of tumor(P>0.05).The expressions of GPR30,ERα and cyclin D1 between any two in PTC tissues was positively correlated(P<0.01).Conclusions:The GPR30 and ERα combined with estrogen can affect the occurrence and development of PTC,which can be set as the potential targets of clinical diagnosis and treatment。
Objective:To explore the expressions and correlations of G-protein coupled receptor 30(GPR30),estrogen receptor α(ERα) and cyclin D1 in papillary thyroid carcinoma(PTC) tissues.Methods:The expression levels of GPR30,ERα and cyclin D1 in 76 PTC samples were detected using immunohistochemical S-P method.The differences of the positive expression rates of GPR30,ERα and cyclin D1,and correlations of GPR30,ERα and cyclin D1 with pathological features were analyzed.Results:The positive expression rates of GPR30,ERα and cyclin D1 in PTC tissues were 67.1%,76.3% and 78.9%,respectively,and the expressions of GPR30,ERα and cyclin D1 in normal thyroid tissues around cancer were not found.The positive expression rates of GPR30 and cyclin D1 in patients with lymph node metastasis were higher than those in patients without metastasis(P<0.05 and P<0.01),which was not related to the age and gender of patients,and size and MACIS score of tumor.The expression rate of ERα in female patients was higher than that in male(P<0.01),and which in patients with tumor diameter ≥ 2 cm or <2 cm was not related to the age of patients,lymph node metastasis and MACIS score of tumor(P>0.05).The expressions of GPR30,ERα and cyclin D1 between any two in PTC tissues was positively correlated(P<0.01).Conclusions:The GPR30 and ERα combined with estrogen can affect the occurrence and development of PTC,which can be set as the potential targets of clinical diagnosis and treatment。
2017, 42(10): 1302-1304,1308.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.003
Abstract:
Objective:To evaluate the effects of atomization inhalation of dexmedetomidine on heme oxygenase-1 expression in lung tissues and Qs/Qt during one-lung ventilation in rats.Methods:Forty-eight male SD rats weighed 300-350 g were randomly divided into the two-lung ventilation group(group C),one-lung ventilation group(group O),intravenous injection dexmedetomidine group(group V) and atomization inhalation dexmedetomidine group(group I)(12 rats each group).The group C was coped with two-lung ventilation for 1 h(tidal volume of 10 mL/kg,ventilatory frequency of 60/min and respiration ratio of 1:2) after endotracheal intubation.The O,V and I groups were treated with right lung ventilation for 1 h(tidal volume of 5 mL/kg,ventilatory frequency of 80/min and respiration ratio of 1:2) after endotracheal intubation.The group V was injected with 5 μg/kg of dexmedetomidine at the speed of 2.5 μg·kg-1·h-1 from before operation to the end of operation.The group I was coped with atomization inhalation of 0.1 μg dexmedetomidine combined with 4 mL 0.9% sodium chloride solution before 10 min of operation,oxygen flow for 2 L/min,and the 1 μg dexmedetomidine was continuously inhaled after endotracheal intubation.The group C and group O were coped with the same volume of 0.9% sodium chloride solution.The arterial blood gas,respiratory index(RI),oxygenation index(OI) and Qs/Qt were detected before mechanical ventilation(T0),at the end of mechanical ventilation(T1) and after 30 min of mechanical ventilation(T2).The contents of TNF-α,IL-6 and IL-10 in lung tissue were detected using ELISA assay,the W/D and expression of heme oxygenase-1(HO-1) were examined,and the pathobiology change of lung tissue was observed.Results:Compared with the group C,the contents of TNF-α,IL-6 and IL-10 in lung tissues and W/D increased,the expression of HO-1 upregulated,the RI at T1and T2 increased,the QI decreased and QS/Qt increased in group O,group V and group I(P<0.05 to P<0.01).Compared with the group O,the contents of TNF-α and IL-6 in lung tissues and W/D decreased,the content of IL-10 increased,the expression of HO-1 upregulated,the RI decreased,the QI increased,the QS/Qt decreased,and the pathological injury alleviated in group V and group I(P<0.01).The differences of all indexes between group V and group I were not statistically significant(P>0.05).Conclusions:The atomization inhalation of dexmedetomidine can attenuate the lung injury induced by one-lung ventilation in rats,the mechanism of which maybe involves in the upregulation of HO-1 expression,and decreasing of Qs/Qt.
Objective:To evaluate the effects of atomization inhalation of dexmedetomidine on heme oxygenase-1 expression in lung tissues and Qs/Qt during one-lung ventilation in rats.Methods:Forty-eight male SD rats weighed 300-350 g were randomly divided into the two-lung ventilation group(group C),one-lung ventilation group(group O),intravenous injection dexmedetomidine group(group V) and atomization inhalation dexmedetomidine group(group I)(12 rats each group).The group C was coped with two-lung ventilation for 1 h(tidal volume of 10 mL/kg,ventilatory frequency of 60/min and respiration ratio of 1:2) after endotracheal intubation.The O,V and I groups were treated with right lung ventilation for 1 h(tidal volume of 5 mL/kg,ventilatory frequency of 80/min and respiration ratio of 1:2) after endotracheal intubation.The group V was injected with 5 μg/kg of dexmedetomidine at the speed of 2.5 μg·kg-1·h-1 from before operation to the end of operation.The group I was coped with atomization inhalation of 0.1 μg dexmedetomidine combined with 4 mL 0.9% sodium chloride solution before 10 min of operation,oxygen flow for 2 L/min,and the 1 μg dexmedetomidine was continuously inhaled after endotracheal intubation.The group C and group O were coped with the same volume of 0.9% sodium chloride solution.The arterial blood gas,respiratory index(RI),oxygenation index(OI) and Qs/Qt were detected before mechanical ventilation(T0),at the end of mechanical ventilation(T1) and after 30 min of mechanical ventilation(T2).The contents of TNF-α,IL-6 and IL-10 in lung tissue were detected using ELISA assay,the W/D and expression of heme oxygenase-1(HO-1) were examined,and the pathobiology change of lung tissue was observed.Results:Compared with the group C,the contents of TNF-α,IL-6 and IL-10 in lung tissues and W/D increased,the expression of HO-1 upregulated,the RI at T1and T2 increased,the QI decreased and QS/Qt increased in group O,group V and group I(P<0.05 to P<0.01).Compared with the group O,the contents of TNF-α and IL-6 in lung tissues and W/D decreased,the content of IL-10 increased,the expression of HO-1 upregulated,the RI decreased,the QI increased,the QS/Qt decreased,and the pathological injury alleviated in group V and group I(P<0.01).The differences of all indexes between group V and group I were not statistically significant(P>0.05).Conclusions:The atomization inhalation of dexmedetomidine can attenuate the lung injury induced by one-lung ventilation in rats,the mechanism of which maybe involves in the upregulation of HO-1 expression,and decreasing of Qs/Qt.
2017, 42(10): 1305-1308.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.004
Abstract:
Objective:To establish the determination method of the serum contents of paeoniflorin and erulic acid in rats treated with intragastric administration of Siwu decoction using high performance liquid chromatography(HPLC).Methods:Twenty-four rats were randomly divided into the control group,low and high dosage of Siwu decoction groups(8 rats each group).The control group,low and high dosage of Siwu decoction groups were treated with intragastric administration of distilled water,0.3 g/mL and 0.6 g/mL Siwu decoction(10 mL/kg) for 7 days.The serum in three groups were harvested from abdominal aorta under chloralic hydras anesthesia,and analyzed using HPLC at the 230 nm of wave length.Results:The linear regression equation,r value and linear range of paeoniflorin and erulic acid were Y=1.019 0X-0.031 & Y=1.026X+0.091,0.999 8 & 0.999 7 and 0.62 to 18.03 μg/mL & 0.32 to 5.11 μg/mL,respectively.The precision,specificity,stability and extraction recovery rate met with the requirement.The serum contents of paeoniflorin and erulic acid in high dosage of Siwu decoction group were significantly higher than those in low dosage of Siwu decoction(P<0.01).Conclusions:This method can determinate the serum levels of paeoniflorin and erulic acid in rats treated with intragastric administration of Siwu decoction.
Objective:To establish the determination method of the serum contents of paeoniflorin and erulic acid in rats treated with intragastric administration of Siwu decoction using high performance liquid chromatography(HPLC).Methods:Twenty-four rats were randomly divided into the control group,low and high dosage of Siwu decoction groups(8 rats each group).The control group,low and high dosage of Siwu decoction groups were treated with intragastric administration of distilled water,0.3 g/mL and 0.6 g/mL Siwu decoction(10 mL/kg) for 7 days.The serum in three groups were harvested from abdominal aorta under chloralic hydras anesthesia,and analyzed using HPLC at the 230 nm of wave length.Results:The linear regression equation,r value and linear range of paeoniflorin and erulic acid were Y=1.019 0X-0.031 & Y=1.026X+0.091,0.999 8 & 0.999 7 and 0.62 to 18.03 μg/mL & 0.32 to 5.11 μg/mL,respectively.The precision,specificity,stability and extraction recovery rate met with the requirement.The serum contents of paeoniflorin and erulic acid in high dosage of Siwu decoction group were significantly higher than those in low dosage of Siwu decoction(P<0.01).Conclusions:This method can determinate the serum levels of paeoniflorin and erulic acid in rats treated with intragastric administration of Siwu decoction.
2017, 42(10): 1309-1313.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.005
Abstract:
Objective:To explore the effects of astragalus injection on the angiogenesis and HIF-1α/VEGF(hypoxia inducible factors-1α/vascular endothelial growth factor) signal pathway in brain tissue of rats with cerebral ischemia reperfusion injury.Methods:Seventy-two rats were randomly divided into the control group,model group and astragalus injection group(24 rats each group).The rat model of cerebral ischemia reperfusion injury models were established by nylon monofilament induced method.Before operation,after operation and after 7 days of adminstration,the neurological scores in all rats were evaluated,and the expression levels of microvessel density(MVD) in brain tissue of rats were detected by immunohistochemical method,the mRNA and protein expression levels of HIF-1α and VEGF in brain tissue of rats were detected by RT-PCR and Western Blotting,respectively.Results:After 7 dyas of adminstration,the neurological score in astragalus injection group was significantly higher than that in model group(P<0.01),and the level of MVD,mRNA and protein expression levels of HIF-1α and VEGF in astragalus injection group were significantly higher than those in model group(P<0.01).Conclusions:Astragalus injection can promote the angiogenesis in brain tissue of rats with cerebral ischemia reperfusion injury,the mechanism of which maybe activate the pathway of HIF-1α/VEGF.
Objective:To explore the effects of astragalus injection on the angiogenesis and HIF-1α/VEGF(hypoxia inducible factors-1α/vascular endothelial growth factor) signal pathway in brain tissue of rats with cerebral ischemia reperfusion injury.Methods:Seventy-two rats were randomly divided into the control group,model group and astragalus injection group(24 rats each group).The rat model of cerebral ischemia reperfusion injury models were established by nylon monofilament induced method.Before operation,after operation and after 7 days of adminstration,the neurological scores in all rats were evaluated,and the expression levels of microvessel density(MVD) in brain tissue of rats were detected by immunohistochemical method,the mRNA and protein expression levels of HIF-1α and VEGF in brain tissue of rats were detected by RT-PCR and Western Blotting,respectively.Results:After 7 dyas of adminstration,the neurological score in astragalus injection group was significantly higher than that in model group(P<0.01),and the level of MVD,mRNA and protein expression levels of HIF-1α and VEGF in astragalus injection group were significantly higher than those in model group(P<0.01).Conclusions:Astragalus injection can promote the angiogenesis in brain tissue of rats with cerebral ischemia reperfusion injury,the mechanism of which maybe activate the pathway of HIF-1α/VEGF.
2017, 42(10): 1313-1317.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.006
Abstract:
Objective:To investigate the active mechanism of the surface marker Cripto-1(CR-1) expression on esophageal cancer stem cell growth.Methods:Eca109 stem cells was isolated from Eca109 cells.The total proteins of HEEC,Eca109 cells and Eca109 stem cells were extracted,and the expression level of CR-1 was detected using Western-blot.The silencing CNE2/CR1- vector targeting CR-1 gene and overexpression vector CNE1/CR-1+ were constructed,and transfected into the Eca109 stem cells using lipofectamine.The control group was set.The transfection efficiency was examined using RT-PCR.The proliferation of Eca109 stem cells in control group,CNE2/CR-1- and CNE1/CR-1+ were detected using MTT.the prognosis of Eca109 stem cells in each group was examined using flow cytometer.The protein expression levels of Akt and p-Akt in each group were detected using Western-blot.Results:The expression levels of CR-1 in Eca109 cells and Eca109 stem cells were significantly higher than that in HEEC cells(P<0.01).RT-PCR results showed that the expression level of CR-1 in CNE2/CR-1-group was significantly lower than that in control group,and the expression level of CR-1 in CNE1/CR-1+ group was significantly higher than that in control group(P<0.01).MTT assay results showed that the cell proliferation in CNE2/CR-1- group was significantly lower than that in control group,and which in CNE1/CR-1+ group was significantly higher than that in control group(P<0.01).Flow cytometry results showed that the apoptosis rate of cells in CNE2/CR-1- group was significantly higher than that in control group,which in CNE1/CR-1+ group was significantly lower than that in control group(P<0.01).Western-blot results showed that the expression level of p-Akt protein in CNE2/CR-1- was significantly lower than that in control group,and the expression level of p-Akt protein in CNE1/CR-1+ group was significantly higher than that tin control group(P<0.01).The expression change of Akt protein was not obvious(P>0.05).Conclusions:The CR-1,an esophageal cancer stem cell marker,may regulate the proliferation and apoptosis of esophageal stem cell by PI3K/Akt signaling pathway.
Objective:To investigate the active mechanism of the surface marker Cripto-1(CR-1) expression on esophageal cancer stem cell growth.Methods:Eca109 stem cells was isolated from Eca109 cells.The total proteins of HEEC,Eca109 cells and Eca109 stem cells were extracted,and the expression level of CR-1 was detected using Western-blot.The silencing CNE2/CR1- vector targeting CR-1 gene and overexpression vector CNE1/CR-1+ were constructed,and transfected into the Eca109 stem cells using lipofectamine.The control group was set.The transfection efficiency was examined using RT-PCR.The proliferation of Eca109 stem cells in control group,CNE2/CR-1- and CNE1/CR-1+ were detected using MTT.the prognosis of Eca109 stem cells in each group was examined using flow cytometer.The protein expression levels of Akt and p-Akt in each group were detected using Western-blot.Results:The expression levels of CR-1 in Eca109 cells and Eca109 stem cells were significantly higher than that in HEEC cells(P<0.01).RT-PCR results showed that the expression level of CR-1 in CNE2/CR-1-group was significantly lower than that in control group,and the expression level of CR-1 in CNE1/CR-1+ group was significantly higher than that in control group(P<0.01).MTT assay results showed that the cell proliferation in CNE2/CR-1- group was significantly lower than that in control group,and which in CNE1/CR-1+ group was significantly higher than that in control group(P<0.01).Flow cytometry results showed that the apoptosis rate of cells in CNE2/CR-1- group was significantly higher than that in control group,which in CNE1/CR-1+ group was significantly lower than that in control group(P<0.01).Western-blot results showed that the expression level of p-Akt protein in CNE2/CR-1- was significantly lower than that in control group,and the expression level of p-Akt protein in CNE1/CR-1+ group was significantly higher than that tin control group(P<0.01).The expression change of Akt protein was not obvious(P>0.05).Conclusions:The CR-1,an esophageal cancer stem cell marker,may regulate the proliferation and apoptosis of esophageal stem cell by PI3K/Akt signaling pathway.
2017, 42(10): 1318-1320,1323.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.007
Abstract:
Objective:To investigate the clinical significance of the detection of circulating tumor cells(CTCs) in peripheral venous blood of breast cancer patients.Methods:The CTCs in peripheral venous blood of 60 patients with breast cancer before new stage treatment were examined using density gradient centrifugation combined with flow cytometry(FCM),and the expression levels of estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor-2(HER-2),p53 and Ki-67 were detected using the immunohistochemical method.Twenty healthy female volunteers were set as the control group at the same time.Results:The positive rate of CTCs in observation group was 38.3%(23/60),the positive CTCs was not detected in control group,and the difference of the positive expression of CTCs between two groups was statistically significant(P<0.01).The differences of the positive expression of CTCs in different ages and pathological types patients were not statistically significant(P>0.05).The difference of the positive expression of CTCs in different TNM stages patients was statistically significant(P<0.01).The positive expression of CTCs in metastatic breast cancer patients was significantly higher than that in nonmetastatic breast cancer patients(P<0.01).The differences of the positive expression of CTCs in patients with different ER,PR,HER-2,Ki-67 and molecular classification were not statistically significant(P>0.05).Conclusions:The CTCs is related to the TNM staging and metastatsis of breast cancer,which has a great significance in the diagnosis,treatment and evaluating prognosis of breast cancer.
Objective:To investigate the clinical significance of the detection of circulating tumor cells(CTCs) in peripheral venous blood of breast cancer patients.Methods:The CTCs in peripheral venous blood of 60 patients with breast cancer before new stage treatment were examined using density gradient centrifugation combined with flow cytometry(FCM),and the expression levels of estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor-2(HER-2),p53 and Ki-67 were detected using the immunohistochemical method.Twenty healthy female volunteers were set as the control group at the same time.Results:The positive rate of CTCs in observation group was 38.3%(23/60),the positive CTCs was not detected in control group,and the difference of the positive expression of CTCs between two groups was statistically significant(P<0.01).The differences of the positive expression of CTCs in different ages and pathological types patients were not statistically significant(P>0.05).The difference of the positive expression of CTCs in different TNM stages patients was statistically significant(P<0.01).The positive expression of CTCs in metastatic breast cancer patients was significantly higher than that in nonmetastatic breast cancer patients(P<0.01).The differences of the positive expression of CTCs in patients with different ER,PR,HER-2,Ki-67 and molecular classification were not statistically significant(P>0.05).Conclusions:The CTCs is related to the TNM staging and metastatsis of breast cancer,which has a great significance in the diagnosis,treatment and evaluating prognosis of breast cancer.
2017, 42(10): 1321-1323.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.008
Abstract:
Objective:To analyze the influencing factor of bronchopulmonary dysplasia(BPD) in premature infants for providing a reference in the clinical prevention and treatment of premature infants with BPD.Methods:Nine hundred and eighty-six premature infants(gestational age <37 weeks and survival ≥ 28 d) were divided into the non-BPD group and BPD group.The data of gender,gestational age,mode of delivery,birth weight,maternal age,arterial occlusion,ventilator-associated pneumonia,pulmonary hemorrhage,anemia,premature infants with hyperbilirubinemia,use oxygen time and hospitalization time in two groups were retrospectively analyzed.Results:Among the 986 premature infants,138 cases with BPD were diagnosed,the incidence rate of which was 14%.The difference of the maternal age between two groups was not statistically significant(P>0.05),and the differences of gestational age,birth weight,use oxygen time and hospitalization time between two groups were statistically significant(P<0.01).The differences of the incidence rate of BPD in different genders of premature infants,and premature infants with or without respiratory distress syndrome were not statistically significant(P>0.05).The differences of the incidence rate of BPD in different gestational age,gestational hypertension,amniotic fluid pollution,pulmonary surfactant use,pulmonary hemorrhage,ADA,anemia and mechanical ventilation in premature infants were statistically significant(P<0.05 to P<0.01).The multiple logistic regression analysis showed that the low gestational age,use pulmonary surfactant,amniotic fluid pollution,PDA and mechanical ventilation were the independent influencing factor in the incidence of BPD of premature infants(P<0.05).Conclusions:The influencing factor of the incidence of BPD of premature infants is more.Among the premature infants with gestational age less than 30 weeks,avoiding amniotic fluid pollution and reasonable reducing mechanical ventilation time can help to reduce the BPD of premature infants.
Objective:To analyze the influencing factor of bronchopulmonary dysplasia(BPD) in premature infants for providing a reference in the clinical prevention and treatment of premature infants with BPD.Methods:Nine hundred and eighty-six premature infants(gestational age <37 weeks and survival ≥ 28 d) were divided into the non-BPD group and BPD group.The data of gender,gestational age,mode of delivery,birth weight,maternal age,arterial occlusion,ventilator-associated pneumonia,pulmonary hemorrhage,anemia,premature infants with hyperbilirubinemia,use oxygen time and hospitalization time in two groups were retrospectively analyzed.Results:Among the 986 premature infants,138 cases with BPD were diagnosed,the incidence rate of which was 14%.The difference of the maternal age between two groups was not statistically significant(P>0.05),and the differences of gestational age,birth weight,use oxygen time and hospitalization time between two groups were statistically significant(P<0.01).The differences of the incidence rate of BPD in different genders of premature infants,and premature infants with or without respiratory distress syndrome were not statistically significant(P>0.05).The differences of the incidence rate of BPD in different gestational age,gestational hypertension,amniotic fluid pollution,pulmonary surfactant use,pulmonary hemorrhage,ADA,anemia and mechanical ventilation in premature infants were statistically significant(P<0.05 to P<0.01).The multiple logistic regression analysis showed that the low gestational age,use pulmonary surfactant,amniotic fluid pollution,PDA and mechanical ventilation were the independent influencing factor in the incidence of BPD of premature infants(P<0.05).Conclusions:The influencing factor of the incidence of BPD of premature infants is more.Among the premature infants with gestational age less than 30 weeks,avoiding amniotic fluid pollution and reasonable reducing mechanical ventilation time can help to reduce the BPD of premature infants.
2017, 42(10): 1324-1326,1329.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.009
Abstract:
Objective:To compare the efficacy and safety of antegrade and retrograde radical retropubic prostatectomy in the treatment of localized prostate cancer.Methods:Seventy patients with localized prostate cancer were randomly divided into the antegrade and retrograde groups(35 cases each group),and treated with antegrade and retrograde radical retropubic prostatectomy,respectively.The age,prostate volume,prostate-specific antigen,Gleason score,peroperative bleeding,operation time,postoperative pathological staging,following-up time,operation complication,and postoperative maximum urinary flow rate,sexual life recovery,biochemical recurrent time and number between two groups were compared.Results:No death in two groups was found.The differences of the age,prostate volume,prostate-specific antigen,Gleason score,pathological staging,following-up time,operation complication,and postoperative maximum urinary flow rate,biochemical recurrent number and sexual life recovery between two groups were not statistically significant(P>0.05).The operation time and peroperative bleeding in antegrade group were significantly less than those in retrograde group(P<0.01),and the time of detecting maximum urinary flow and biochemical recurrent time in antegrade group were significantly shorter than those in retrograde group(P<0.05 to P<0.01).Conclusions:Both antegrade and retrograde radical retropubic prostatectomy in the treatment of localized prostate cancer are safe and effective,and the peroperative bleeding and operation time in patients treated with antegrade radical retropublic prostatectomy are less and shorter compared with retrograde radical retropublic prostatectomy.
Objective:To compare the efficacy and safety of antegrade and retrograde radical retropubic prostatectomy in the treatment of localized prostate cancer.Methods:Seventy patients with localized prostate cancer were randomly divided into the antegrade and retrograde groups(35 cases each group),and treated with antegrade and retrograde radical retropubic prostatectomy,respectively.The age,prostate volume,prostate-specific antigen,Gleason score,peroperative bleeding,operation time,postoperative pathological staging,following-up time,operation complication,and postoperative maximum urinary flow rate,sexual life recovery,biochemical recurrent time and number between two groups were compared.Results:No death in two groups was found.The differences of the age,prostate volume,prostate-specific antigen,Gleason score,pathological staging,following-up time,operation complication,and postoperative maximum urinary flow rate,biochemical recurrent number and sexual life recovery between two groups were not statistically significant(P>0.05).The operation time and peroperative bleeding in antegrade group were significantly less than those in retrograde group(P<0.01),and the time of detecting maximum urinary flow and biochemical recurrent time in antegrade group were significantly shorter than those in retrograde group(P<0.05 to P<0.01).Conclusions:Both antegrade and retrograde radical retropubic prostatectomy in the treatment of localized prostate cancer are safe and effective,and the peroperative bleeding and operation time in patients treated with antegrade radical retropublic prostatectomy are less and shorter compared with retrograde radical retropublic prostatectomy.
2017, 42(10): 1327-1329.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.010
Abstract:
Objective:To explore the surgical technique of the cranioplasty of digital forming titanium mesh.Methods:The clinical data of 202 skull defect patients treated with cranioplasty of digital forming titanium mesh were retrospectively analyzed from March 2006 to March 2016.Results:All patients recovered well,the head appearance is satisfactory,and the results of CT showed that the titanium mesh and skull is closely fit.The neurological dysfunction of all patients was significantly improved.The scalp effusion in 6 cases,local infection in 1 case,small intracerebral hematoma in 2 cases,preoperative epidural hematoma in 3 cases,a large number of epidural hematoma after shunt in 2 cases,and postoperative grand mal epilepsy in 2 cases were identified.No titanium mesh exposure,loosening,deformation,chewing pain and mouth restriction in all patients were observed.Conclusions:Improving surgical technique can significantly reduce the complications of cranioplasty of digital forming titanium mesh.
Objective:To explore the surgical technique of the cranioplasty of digital forming titanium mesh.Methods:The clinical data of 202 skull defect patients treated with cranioplasty of digital forming titanium mesh were retrospectively analyzed from March 2006 to March 2016.Results:All patients recovered well,the head appearance is satisfactory,and the results of CT showed that the titanium mesh and skull is closely fit.The neurological dysfunction of all patients was significantly improved.The scalp effusion in 6 cases,local infection in 1 case,small intracerebral hematoma in 2 cases,preoperative epidural hematoma in 3 cases,a large number of epidural hematoma after shunt in 2 cases,and postoperative grand mal epilepsy in 2 cases were identified.No titanium mesh exposure,loosening,deformation,chewing pain and mouth restriction in all patients were observed.Conclusions:Improving surgical technique can significantly reduce the complications of cranioplasty of digital forming titanium mesh.
2017, 42(10): 1330-1332.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.011
Abstract:
Objective:To investigate the expression level of serum decoy receptor 3(DcR3) in patients with rheumatoid arthritis(RA),and its clinical significance.Methods:Sixty RA patients and 30 healthy donators were divided into the RA group and control group,respectively,and the RA group were subdivided into the low activity group and activity group according to the disease activity index.The serum levels of DcR3 and IFN-γ in two groups were detected using enzyme-linked immunosorbent assay(ELISA).Results:The levels of DcR3 and IFN-γ in RA group were significantly higher than those in control group(P<0.01).The differences of the serum levels of DcR3 and IFN-γ between activity group,low activity group and control group were statistically significant(P<0.01).The serum levels of DcR3 and IFN-γ in activity group were significantly higher than those in activity group and control group(P<0.01).The serum level IFN-γ in low activity group was significantly higher than that in control group(P<0.01).The serum level of DcR3 in RA patients was positive correlation with the level of IFN-γ(P<0.05).Conclusions:The expression levels of DcR3 and IFN-γ increase in RA patients,and which is positive correlation with the level of IFN-γ.DcR3 may be involved in the pathogenesis of RA,and is related to the disease activity.
Objective:To investigate the expression level of serum decoy receptor 3(DcR3) in patients with rheumatoid arthritis(RA),and its clinical significance.Methods:Sixty RA patients and 30 healthy donators were divided into the RA group and control group,respectively,and the RA group were subdivided into the low activity group and activity group according to the disease activity index.The serum levels of DcR3 and IFN-γ in two groups were detected using enzyme-linked immunosorbent assay(ELISA).Results:The levels of DcR3 and IFN-γ in RA group were significantly higher than those in control group(P<0.01).The differences of the serum levels of DcR3 and IFN-γ between activity group,low activity group and control group were statistically significant(P<0.01).The serum levels of DcR3 and IFN-γ in activity group were significantly higher than those in activity group and control group(P<0.01).The serum level IFN-γ in low activity group was significantly higher than that in control group(P<0.01).The serum level of DcR3 in RA patients was positive correlation with the level of IFN-γ(P<0.05).Conclusions:The expression levels of DcR3 and IFN-γ increase in RA patients,and which is positive correlation with the level of IFN-γ.DcR3 may be involved in the pathogenesis of RA,and is related to the disease activity.
2017, 42(10): 1333-1336.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.012
Abstract:
Objective:To investigate the clinical significance and biological function of microRNA(miR-106b) expression in retinoblastoma,and effects of miR-106b on the viability,proliferation and apoptosis of retinoblastoma cells.Methods:The expressions of miR-106b in 33 specimens of retinoblastoma tissue and precancerous tissues were detected using qRT-PCR,and the correlation of which with clinicopathologic feature was analyzed.The miR-106b mimics or inhibitors were transfected into the retinoblastoma cells.The viability,proliferation and apoptosis of retinoblastoma cells were detected using MTT,BrdU and flow cytometry,and the expression of Rb1 protein was detected using Western blot.Results:The expression of miR-106b in retinoblastoma tissue was significantly higher than that in precancerous tissue(P<0.01),which was not correlated with optic nerve infiltration(P<0.05).The expressions of miR-106b in retinoblastoma cells Y79 and HXO-Rb44 were significantly higher than that in normal retinal vascular endothelial cells ACBRI-181(P<0.01).MiR-106b inhibitor could obviously inhibit the expression of miR-106b in HXO-Rb44 cells(P<0.01),and significantly lead to the decreasing of cell viability,proliferation and increasing of percentage of cell apoptosis(P<0.05).The decreasing expression of miR-106b in HXO-Rb44 cells could significantly increase the Rb1 protein expression,and the increasing expression of miR-106b in Y79 cells could significantly decrease the Rb1 protein expression.Conclusions:The expression of miR-106b increases in retinoblastoma tissue,which is correlated with the adverse clinicopathological features of retinoblastoma.MiR-106b can strengthen the viability and proliferation,and reduce the apoptosis of retinoblastoma cells and the expression of Rb1 in retinoblastoma cells.MiR-106b may play a key role in the development and progression of retinoblastoma.
Objective:To investigate the clinical significance and biological function of microRNA(miR-106b) expression in retinoblastoma,and effects of miR-106b on the viability,proliferation and apoptosis of retinoblastoma cells.Methods:The expressions of miR-106b in 33 specimens of retinoblastoma tissue and precancerous tissues were detected using qRT-PCR,and the correlation of which with clinicopathologic feature was analyzed.The miR-106b mimics or inhibitors were transfected into the retinoblastoma cells.The viability,proliferation and apoptosis of retinoblastoma cells were detected using MTT,BrdU and flow cytometry,and the expression of Rb1 protein was detected using Western blot.Results:The expression of miR-106b in retinoblastoma tissue was significantly higher than that in precancerous tissue(P<0.01),which was not correlated with optic nerve infiltration(P<0.05).The expressions of miR-106b in retinoblastoma cells Y79 and HXO-Rb44 were significantly higher than that in normal retinal vascular endothelial cells ACBRI-181(P<0.01).MiR-106b inhibitor could obviously inhibit the expression of miR-106b in HXO-Rb44 cells(P<0.01),and significantly lead to the decreasing of cell viability,proliferation and increasing of percentage of cell apoptosis(P<0.05).The decreasing expression of miR-106b in HXO-Rb44 cells could significantly increase the Rb1 protein expression,and the increasing expression of miR-106b in Y79 cells could significantly decrease the Rb1 protein expression.Conclusions:The expression of miR-106b increases in retinoblastoma tissue,which is correlated with the adverse clinicopathological features of retinoblastoma.MiR-106b can strengthen the viability and proliferation,and reduce the apoptosis of retinoblastoma cells and the expression of Rb1 in retinoblastoma cells.MiR-106b may play a key role in the development and progression of retinoblastoma.
2017, 42(10): 1337-1339.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.013
Abstract:
Objective:To analyze the effects of maternal body mass index(BMI) on pregnant women and newborn.Methods:Nine hundred pregnant women were divided into the lean group (BMI<20 kg/m2),normal group(20kg/m2 ≤ BMI<25kg/m2) and obesity group(BMI ≥ 25 kg/m2) according to the BMI before pregnancy.The pregnant women were divided into the less than 15kg group,15 to 20kg group and more than 20kg group according to the body mass increasing degree during pregnancy.The effects of maternal BMI before pregnancy and body mass increasing on the delivery mode and outcome of pregnant women were recorded.Results:The spontaneous labor rate and cesarean section rate in lean group,normal group and obesity group decreased and increased successively,respectively(P<0.01).With the increasing of body weight of pregnant women,the cesarean section rate and macrosomia birth rate showed an upward trend(P<0.01).The detection rates of gestational hypertension,gestational diabetes,labor block,postpartum hemorrhage and neonatal asphyxia in lean group,normal group and obesity group increased gradually(P<0.01).With the increasing of body mass during pregnancy,the detection rates of gestational hypertension,gestational diabetes,postpartum hemorrhage in obesity group increased gradually(P<0.01).Conclusions:Obesity and excessive increasing weight during pregnancy can improve the risk of gestational hypertension and diabetes,which also can significantly increase the cesarean section rate,fetal macrosomia occurrence,and adverse pregnancy outcomes(such as labor block,postpartum hemorrhage and neonatal asphyxia).
Objective:To analyze the effects of maternal body mass index(BMI) on pregnant women and newborn.Methods:Nine hundred pregnant women were divided into the lean group (BMI<20 kg/m2),normal group(20kg/m2 ≤ BMI<25kg/m2) and obesity group(BMI ≥ 25 kg/m2) according to the BMI before pregnancy.The pregnant women were divided into the less than 15kg group,15 to 20kg group and more than 20kg group according to the body mass increasing degree during pregnancy.The effects of maternal BMI before pregnancy and body mass increasing on the delivery mode and outcome of pregnant women were recorded.Results:The spontaneous labor rate and cesarean section rate in lean group,normal group and obesity group decreased and increased successively,respectively(P<0.01).With the increasing of body weight of pregnant women,the cesarean section rate and macrosomia birth rate showed an upward trend(P<0.01).The detection rates of gestational hypertension,gestational diabetes,labor block,postpartum hemorrhage and neonatal asphyxia in lean group,normal group and obesity group increased gradually(P<0.01).With the increasing of body mass during pregnancy,the detection rates of gestational hypertension,gestational diabetes,postpartum hemorrhage in obesity group increased gradually(P<0.01).Conclusions:Obesity and excessive increasing weight during pregnancy can improve the risk of gestational hypertension and diabetes,which also can significantly increase the cesarean section rate,fetal macrosomia occurrence,and adverse pregnancy outcomes(such as labor block,postpartum hemorrhage and neonatal asphyxia).
2017, 42(10): 1340-1343.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.014
Abstract:
Objective:To detect the methylation of CDH13 gene in patients with acute myeloid leukemia(AML),and explore the relationships with clinical features and prognosis of AML patients.Methods:The methylation status of CDH13 promoter gene in bone marrow from 34 patients with AML(AML group) and 22 nonmalignant tumor patients with iron deficiency anemia(control group) were detected using the methylation specific polymerase chain reaction(PCR).The expressions of CDH13 mRNA in two groups were detected using semi-quantitative reverse transcription PCR.All patients were followed-up for 2 years,and the overall survival time of patients were calculated.Results:The CDH13 gene methylation positive rate of AML group(61.76%) was higher than that of control group(27.27%)(P<0.05).The relative content of CDH13 mRNA in AML group(0.355±0.109) was significantly lower than that in control group(0.745±0.271)(P<0.01).The differences of the methylation status of CDH13 gene in AML patients of indifferent gender,age and clinical typing were not statistically significant(P>0.05).Kaplan-Meier survival curve analysis showed that the overall survival time in AML patients with positive methylation of CDH13 gene[(12.4±3.3)months] was significantly shorter than that in AML patients with negative methylation of CDH13 gene[(18.6±4.1) months](P<0.01).Conclusions:The methylation phenomenon of CDH13 gene can be found in patients with AML,and detecting the CDH13 gene expression can help to judge the prognosis of patients.
Objective:To detect the methylation of CDH13 gene in patients with acute myeloid leukemia(AML),and explore the relationships with clinical features and prognosis of AML patients.Methods:The methylation status of CDH13 promoter gene in bone marrow from 34 patients with AML(AML group) and 22 nonmalignant tumor patients with iron deficiency anemia(control group) were detected using the methylation specific polymerase chain reaction(PCR).The expressions of CDH13 mRNA in two groups were detected using semi-quantitative reverse transcription PCR.All patients were followed-up for 2 years,and the overall survival time of patients were calculated.Results:The CDH13 gene methylation positive rate of AML group(61.76%) was higher than that of control group(27.27%)(P<0.05).The relative content of CDH13 mRNA in AML group(0.355±0.109) was significantly lower than that in control group(0.745±0.271)(P<0.01).The differences of the methylation status of CDH13 gene in AML patients of indifferent gender,age and clinical typing were not statistically significant(P>0.05).Kaplan-Meier survival curve analysis showed that the overall survival time in AML patients with positive methylation of CDH13 gene[(12.4±3.3)months] was significantly shorter than that in AML patients with negative methylation of CDH13 gene[(18.6±4.1) months](P<0.01).Conclusions:The methylation phenomenon of CDH13 gene can be found in patients with AML,and detecting the CDH13 gene expression can help to judge the prognosis of patients.
2017, 42(10): 1343-1346.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.015
Abstract:
Objective:To explore the expressions of FMNL2 and RAC1 in colorectal cancer(CRC) tissues and corresponding precancerous lesions,and correlation of the expressions with clinical pathological parameters.Methods:The mRNA and protein expressions of FMNL2 and RAC1 in CRC tissues and corresponding precancerous lesions were detected by real-time PCR and immunohistochemistry(IHC) method.Results:The mRNA expressions of FMNL2 and RAC1 in CRC tissues were higher than those in corresponding precancerous lesions(P<0.01).The positive rates of FMNL2 and RAC1 in CRC tissues(61.7% and 68.3%) were higher than those in corresponding precancerous lesions(20.8% and 31.7%),respectively(P<0.05).The expressions of FMNL2 and RAC1 were closely related to the tumor metastasis,lymph node metastasis and TNM staging(P<0.05 to P<0.01),but which was not related to the gender and age of patients,and size,differentiation and invasion of tumor(P>0.05).The expression of FMNL2 was positively correlated with RAC1(r=0.495,P<0.05).Conclusions:The expressions of FMNL2 and RAC1 in CRC tissues are high,which is closely related to the tumor metastasis.The combined detection of FMNL2 and RAC1 have important significances in predicting tumor metastasis and prognosis of tumor.
Objective:To explore the expressions of FMNL2 and RAC1 in colorectal cancer(CRC) tissues and corresponding precancerous lesions,and correlation of the expressions with clinical pathological parameters.Methods:The mRNA and protein expressions of FMNL2 and RAC1 in CRC tissues and corresponding precancerous lesions were detected by real-time PCR and immunohistochemistry(IHC) method.Results:The mRNA expressions of FMNL2 and RAC1 in CRC tissues were higher than those in corresponding precancerous lesions(P<0.01).The positive rates of FMNL2 and RAC1 in CRC tissues(61.7% and 68.3%) were higher than those in corresponding precancerous lesions(20.8% and 31.7%),respectively(P<0.05).The expressions of FMNL2 and RAC1 were closely related to the tumor metastasis,lymph node metastasis and TNM staging(P<0.05 to P<0.01),but which was not related to the gender and age of patients,and size,differentiation and invasion of tumor(P>0.05).The expression of FMNL2 was positively correlated with RAC1(r=0.495,P<0.05).Conclusions:The expressions of FMNL2 and RAC1 in CRC tissues are high,which is closely related to the tumor metastasis.The combined detection of FMNL2 and RAC1 have important significances in predicting tumor metastasis and prognosis of tumor.
2017, 42(10): 1347-1348,1352.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.016
Abstract:
Objective:To investigate the effects of fibrinolytic enzyme on inflammatory factors in non-surgical patients with non-ST-segment elevation myocardial infarction.Methods:Eighty patients with non-ST-segment elevation myocardial infarction and normal physical examinees were divided into the treatment group and control group,respectively.The serum levels of high-sensitivity c-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α) and interleukin 6(IL-6) in two groups were observed.Eighty patients with non-ST-segment elevation myocardial infarction were randomly subdivided into the routine treatment group and fibrinolytic enzyme treatment group,and the effects of fibrinolytic enzyme on the levels of hs-CRP,TNF-α and IL-6 were observed.Results:Compared with the control group,the serum levels of hs-CRP,TNF-α and IL-6 in observation group at admission significantly increased(P<0.01).After treatment,the serum levels of hs-CRP,TNF-α and IL-6 in fibrinolytic enzyme treatment group significantly decreased compared with the routine treatment group(P<0.01).Conclusions:The non-ST-segment elevation myocardial infarction involves the immune inflammatory injury,and fibrinolytic enzyme can inhibit the expression of immune inflammatory factors.
Objective:To investigate the effects of fibrinolytic enzyme on inflammatory factors in non-surgical patients with non-ST-segment elevation myocardial infarction.Methods:Eighty patients with non-ST-segment elevation myocardial infarction and normal physical examinees were divided into the treatment group and control group,respectively.The serum levels of high-sensitivity c-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α) and interleukin 6(IL-6) in two groups were observed.Eighty patients with non-ST-segment elevation myocardial infarction were randomly subdivided into the routine treatment group and fibrinolytic enzyme treatment group,and the effects of fibrinolytic enzyme on the levels of hs-CRP,TNF-α and IL-6 were observed.Results:Compared with the control group,the serum levels of hs-CRP,TNF-α and IL-6 in observation group at admission significantly increased(P<0.01).After treatment,the serum levels of hs-CRP,TNF-α and IL-6 in fibrinolytic enzyme treatment group significantly decreased compared with the routine treatment group(P<0.01).Conclusions:The non-ST-segment elevation myocardial infarction involves the immune inflammatory injury,and fibrinolytic enzyme can inhibit the expression of immune inflammatory factors.
2017, 42(10): 1349-1352.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.017
Abstract:
Objective:To observe the effects of L-carnitine injection on inflammation and red blood cell(RBC) immune function in diabetic nephropathy(DN) patients treated with maintenance hemodialysis(MHD).Methods:Eighty-nine MHD patients were randomly into the observation group(47 cases) and control group(42 cases).Two groups were treated with peritoneal dialysis,2 to 3 times a week,4 h each time.The observation group was intravenously injected with L-carnitine for 5 min before the end of the dialysis,1 g each time.The inflammatory factors and RBC immune indexes in two groups were detected after 1 and 3 months of treatment.Results:With the prolonging of treatment,the levels of IL-6,TNF-α,and hs-CRP in observation group declined gradually,and the decreasing degree of those in control group was not obvious.The levels of inflammatory factors in observation group after 1 month and 3 months of treatment were significantly lower than those in control group(P< 0.01).After treatment,the percentages of RBC-C3bRR wreath and CD35 positive RBC,and level of SOD gradually increased,and the percentage of RBC-ICR wreath declined gradually in two groups.After 1 month and 3 months of treatment,the percentages of RBC-C3bRR wreath and CD35 positive RBC in observation group were significantly higher than those in control group(P<0.05 to P<0.01),but the percentage of RBC-ICR wreath in observation group after 1 month and 3 months of treatment was significantly lower than that in control group(P<0.01).Conclusions:The exogenous L-carnitine in DN patients treated with MHD can obviously improve micro-inflammation status,and erythrocyte immune function.
Objective:To observe the effects of L-carnitine injection on inflammation and red blood cell(RBC) immune function in diabetic nephropathy(DN) patients treated with maintenance hemodialysis(MHD).Methods:Eighty-nine MHD patients were randomly into the observation group(47 cases) and control group(42 cases).Two groups were treated with peritoneal dialysis,2 to 3 times a week,4 h each time.The observation group was intravenously injected with L-carnitine for 5 min before the end of the dialysis,1 g each time.The inflammatory factors and RBC immune indexes in two groups were detected after 1 and 3 months of treatment.Results:With the prolonging of treatment,the levels of IL-6,TNF-α,and hs-CRP in observation group declined gradually,and the decreasing degree of those in control group was not obvious.The levels of inflammatory factors in observation group after 1 month and 3 months of treatment were significantly lower than those in control group(P< 0.01).After treatment,the percentages of RBC-C3bRR wreath and CD35 positive RBC,and level of SOD gradually increased,and the percentage of RBC-ICR wreath declined gradually in two groups.After 1 month and 3 months of treatment,the percentages of RBC-C3bRR wreath and CD35 positive RBC in observation group were significantly higher than those in control group(P<0.05 to P<0.01),but the percentage of RBC-ICR wreath in observation group after 1 month and 3 months of treatment was significantly lower than that in control group(P<0.01).Conclusions:The exogenous L-carnitine in DN patients treated with MHD can obviously improve micro-inflammation status,and erythrocyte immune function.
2017, 42(10): 1353-1355.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.018
Abstract:
Objective:To study the value of serum glactin-3,HMGB-1 and TSGF in the diagnosis of thyroid carcinoma.Methods:A total of 60 patients with thyroid carcinoma and 60 patients with thyroid adenoma were selected as the subjects,and the healthy subjects were selected as normal control group(n=60).The levels of serum glactin-3,HMGB-1 and TSGF were detected by enzyme-linked immunosorbent assay (ELISA).The levels of serum glactin-3,HMGB-1 and TSGF in thyroid carcinoma,thyroid adenoma and normal thyroid group were compared.The sensitivity and specificity of thyroid carcinoma between the three indexes were compared with the combined detection of thyroid carcinoma.Results:The levels of glactin-3,HMGB-1 and TSGF in thyroid cancer group were significantly higher than those in thyroid adenoma group(P<0.01),while those in thyroid adenoma group were significantly higher than those in control group(P<0.01).The positive rates and joint positive rates of glactin-3,HMGB-1 and TSGF in thyroid cancer group were significantly higher than those in thyroid adenoma group(P<0.01).The AUC,sensitivity and specificity of the three markers in the diagnosis of thyroid cancer were 0.86,93.3% and 91.7%,which were significantly higher than the sensitivity and specificity of each index(P<0.01).Conclusions:The combined detection of glactin-3,HMGB-1 and TSGF of thyroid cancer had good sensitivity and specificity.
Objective:To study the value of serum glactin-3,HMGB-1 and TSGF in the diagnosis of thyroid carcinoma.Methods:A total of 60 patients with thyroid carcinoma and 60 patients with thyroid adenoma were selected as the subjects,and the healthy subjects were selected as normal control group(n=60).The levels of serum glactin-3,HMGB-1 and TSGF were detected by enzyme-linked immunosorbent assay (ELISA).The levels of serum glactin-3,HMGB-1 and TSGF in thyroid carcinoma,thyroid adenoma and normal thyroid group were compared.The sensitivity and specificity of thyroid carcinoma between the three indexes were compared with the combined detection of thyroid carcinoma.Results:The levels of glactin-3,HMGB-1 and TSGF in thyroid cancer group were significantly higher than those in thyroid adenoma group(P<0.01),while those in thyroid adenoma group were significantly higher than those in control group(P<0.01).The positive rates and joint positive rates of glactin-3,HMGB-1 and TSGF in thyroid cancer group were significantly higher than those in thyroid adenoma group(P<0.01).The AUC,sensitivity and specificity of the three markers in the diagnosis of thyroid cancer were 0.86,93.3% and 91.7%,which were significantly higher than the sensitivity and specificity of each index(P<0.01).Conclusions:The combined detection of glactin-3,HMGB-1 and TSGF of thyroid cancer had good sensitivity and specificity.
2017, 42(10): 1356-1358,1362.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.019
Abstract:
Objective:To investigate the effects of Shenqifuzheng injection(SFI) on bone marrow hematopoietic and immune function in patients with acute lymphoblastic leukemia(ALL) before and after chemotherapy.Methods:Sixty ALL patients were randomly divided into the observation group and control group(30 cases each group).Two groups were treated with chemotherapy,and the observation group were additionally treated with SFI.The blood routine,and T and B lymphocyte subsets in two groups were observed before and after treatment.Results:The effective rate in observation group and control group were 90%,and 86.67%,respectively,and the difference of which was not statistically significant(P>0.05).During two weeks of chemotherapy and after 1 week of chemotherapy,the counts of white blood cell and red blood cell,and hemoglobin level in observation group were significantly higher than those in control group(P<0.05~P<0.0),and the ratios of CD3+,CD4+ and CD4+/CD8+ in observation group after chemotherapy were better than those in control group(P<0.05).The differences of the levels of blood platelet and CD3-CD19+ between two groups were not statistically significant(P>0.05).Conclusions:The treatment of ALL with SFI can effectively improve the myelosuppression induced by chemotherapy,enhance the immunologic function,and promote the recovery of patients after chemotherapy.
Objective:To investigate the effects of Shenqifuzheng injection(SFI) on bone marrow hematopoietic and immune function in patients with acute lymphoblastic leukemia(ALL) before and after chemotherapy.Methods:Sixty ALL patients were randomly divided into the observation group and control group(30 cases each group).Two groups were treated with chemotherapy,and the observation group were additionally treated with SFI.The blood routine,and T and B lymphocyte subsets in two groups were observed before and after treatment.Results:The effective rate in observation group and control group were 90%,and 86.67%,respectively,and the difference of which was not statistically significant(P>0.05).During two weeks of chemotherapy and after 1 week of chemotherapy,the counts of white blood cell and red blood cell,and hemoglobin level in observation group were significantly higher than those in control group(P<0.05~P<0.0),and the ratios of CD3+,CD4+ and CD4+/CD8+ in observation group after chemotherapy were better than those in control group(P<0.05).The differences of the levels of blood platelet and CD3-CD19+ between two groups were not statistically significant(P>0.05).Conclusions:The treatment of ALL with SFI can effectively improve the myelosuppression induced by chemotherapy,enhance the immunologic function,and promote the recovery of patients after chemotherapy.
2017, 42(10): 1359-1362.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.020
Abstract:
Objective:To investigate the intracranial pressure changes in hypertensive cerebral hemorrhage patients treated with drilling and craniotomy drainage,and its correlation with postoperative cognitive dysfunction.Methods:One hundred and six patients with hypertensive cerebral hemorrhage were randomly divided into the craniotomy group and drilling group using the random number table method(53 cases each group).The craniotomy group and drilling group were treated with traditional craniotomy drainage and minimally invasive drilling drainage,respectively.The postoperative neurologic deficit and cognitive function recovery after 1h,12h,24h,72h and 1w of operation between two groups were compared.Results:The intracranial pressure after 1h,12h,24h,72h and 1w of operation in drilling group were significantly higher than those in craniotomy group(P<0.01).After 6 months of operation,the NIHSS score in drilling group was lower than that in craniotomy group(P<0.01),and the NIHSS scores of two groups were significantly lower than that before operation(P<0.01).After 1 month of operation,the levels of NSE and BDNF in drilling group were lower than those in craniotomy group(P<0.01),the levels of NSE and BDNF in two groups after 1 month of operation were significantly lower and higher than those after 3d of operation,respectively(P<0.01).After 6 months of operation,the cognitive dysfunction in two groups were improved compared before operation(P<0.01).The distribution of MMSE score in drilling group was better than that in craniotomy group(P<0.01),The rate of cognitive impairment in drilling group was significantly lower than that in craniotomy group(P<0.05).Conclusions:In the treatment of hypertensive cerebral hemorrhage,the craniotomy drainage has advantage in reducing intracranial pressure,and the drilling drainage has advantage in improving nerve function and cognitive function.
Objective:To investigate the intracranial pressure changes in hypertensive cerebral hemorrhage patients treated with drilling and craniotomy drainage,and its correlation with postoperative cognitive dysfunction.Methods:One hundred and six patients with hypertensive cerebral hemorrhage were randomly divided into the craniotomy group and drilling group using the random number table method(53 cases each group).The craniotomy group and drilling group were treated with traditional craniotomy drainage and minimally invasive drilling drainage,respectively.The postoperative neurologic deficit and cognitive function recovery after 1h,12h,24h,72h and 1w of operation between two groups were compared.Results:The intracranial pressure after 1h,12h,24h,72h and 1w of operation in drilling group were significantly higher than those in craniotomy group(P<0.01).After 6 months of operation,the NIHSS score in drilling group was lower than that in craniotomy group(P<0.01),and the NIHSS scores of two groups were significantly lower than that before operation(P<0.01).After 1 month of operation,the levels of NSE and BDNF in drilling group were lower than those in craniotomy group(P<0.01),the levels of NSE and BDNF in two groups after 1 month of operation were significantly lower and higher than those after 3d of operation,respectively(P<0.01).After 6 months of operation,the cognitive dysfunction in two groups were improved compared before operation(P<0.01).The distribution of MMSE score in drilling group was better than that in craniotomy group(P<0.01),The rate of cognitive impairment in drilling group was significantly lower than that in craniotomy group(P<0.05).Conclusions:In the treatment of hypertensive cerebral hemorrhage,the craniotomy drainage has advantage in reducing intracranial pressure,and the drilling drainage has advantage in improving nerve function and cognitive function.
2017, 42(10): 1363-1364,1367.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.021
Abstract:
Objective:To investigate the effectiveness and prognosis of microsurgery in the treatment of cavernous angiomas in brain stem.Methods:The remission of clinical symptoms in 12 patients with cavernous hemangioma were observed after operation.The quality of life in all cases between before and after operation was compared using the European cancer research and treatment group scale.Results:The incidence rates of headache,vomiting and ataxia in all patients reduced after operation(P<0.05).The total score of life quality and each dimension score in all patients between before operation,after 3,6 and 12 months of operation were different,and the scores of life quality after 3,6 and 12 months of operation were higher than that of before operation(P<0.05).Conclusions:The effect of microsurgical treatment of cavernous hemangioma in brain stem is good,which can improve the prognosis and the quality of life of patients after operation.
Objective:To investigate the effectiveness and prognosis of microsurgery in the treatment of cavernous angiomas in brain stem.Methods:The remission of clinical symptoms in 12 patients with cavernous hemangioma were observed after operation.The quality of life in all cases between before and after operation was compared using the European cancer research and treatment group scale.Results:The incidence rates of headache,vomiting and ataxia in all patients reduced after operation(P<0.05).The total score of life quality and each dimension score in all patients between before operation,after 3,6 and 12 months of operation were different,and the scores of life quality after 3,6 and 12 months of operation were higher than that of before operation(P<0.05).Conclusions:The effect of microsurgical treatment of cavernous hemangioma in brain stem is good,which can improve the prognosis and the quality of life of patients after operation.
2017, 42(10): 1365-1367.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.022
Abstract:
Objective:To investigate the clinical efficacy and safety of Huangqi Dihuang decoction combined with cyclophosphamide in the treatment of refractory nephrotic syndrome.Methods:Ninety-six patients with refractory nephrotic syndrome were randomly divided into the observation group and control group(48 cases each group).The control group was treated with cyclophosphamide,and the observation group was treated with Huangqi Dihuang decoction based on the control group for 6 months.The effective rate of treatment,levels of urea nitrogen(BUN),serum creatinine(Scr),24 h urinary protein,interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) before and after treatment,and adverse reactions between the two groups were compared.Results:The total effective rate in observation group(95.83%) was higher than that in control group(79.17%)(P<0.05).Compared with before treatment,the levels of BUN and Scr,24 h urine protein,IL-6 and TNF-α in two groups significantly decreased after treatment (P<0.01),and the decreasing levels of BUN and Scr,24 h urine protein,IL-6 and TNF-α in observation group were significantly lower than those in control group(P<0.01).No obvious adverse drug reactions in two groups were found.Conclusions:Huangqi Dihuang decoction combined with cyclophosphamide in the treatment of refractory nephrotic syndrome is effective and safe,which may be related to the improvement of renal function and decreasing levels of 24 h urinary protein,IL-6 and TNF-α.
Objective:To investigate the clinical efficacy and safety of Huangqi Dihuang decoction combined with cyclophosphamide in the treatment of refractory nephrotic syndrome.Methods:Ninety-six patients with refractory nephrotic syndrome were randomly divided into the observation group and control group(48 cases each group).The control group was treated with cyclophosphamide,and the observation group was treated with Huangqi Dihuang decoction based on the control group for 6 months.The effective rate of treatment,levels of urea nitrogen(BUN),serum creatinine(Scr),24 h urinary protein,interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) before and after treatment,and adverse reactions between the two groups were compared.Results:The total effective rate in observation group(95.83%) was higher than that in control group(79.17%)(P<0.05).Compared with before treatment,the levels of BUN and Scr,24 h urine protein,IL-6 and TNF-α in two groups significantly decreased after treatment (P<0.01),and the decreasing levels of BUN and Scr,24 h urine protein,IL-6 and TNF-α in observation group were significantly lower than those in control group(P<0.01).No obvious adverse drug reactions in two groups were found.Conclusions:Huangqi Dihuang decoction combined with cyclophosphamide in the treatment of refractory nephrotic syndrome is effective and safe,which may be related to the improvement of renal function and decreasing levels of 24 h urinary protein,IL-6 and TNF-α.
2017, 42(10): 1368-1370,1373.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.023
Abstract:
Objective:To compare the reset effects and knee joint function of sustaining plate(SP),locking plate(LP) and double locking plate in treating complex tibial plateau fracture.Methods:The coronal fractures of internal condyle in 46 patients with complex tibial plateau fracture were examined using CT.The patients were divided into the SP group(12 cases),LP group(14 cases) and bilateral LP group(20 cases).The operation time,bleeding loss,postoperative healing,knee jiont function and complications between three groups were compared.Results:The operation time,bleeding loss and healing time in SP group were significantly higher than those in other two groups(P<0.01 to P<0.05). The differences of the excellent and good rate of postoperative knee joint function and incidence rate of complications between three groups were not statistically significant(P>0.05).Conclusions:The effect of the bilateral LP in treating the coronal fractures of internal condyle in patients with complex tibial plateau fractures is better.
Objective:To compare the reset effects and knee joint function of sustaining plate(SP),locking plate(LP) and double locking plate in treating complex tibial plateau fracture.Methods:The coronal fractures of internal condyle in 46 patients with complex tibial plateau fracture were examined using CT.The patients were divided into the SP group(12 cases),LP group(14 cases) and bilateral LP group(20 cases).The operation time,bleeding loss,postoperative healing,knee jiont function and complications between three groups were compared.Results:The operation time,bleeding loss and healing time in SP group were significantly higher than those in other two groups(P<0.01 to P<0.05). The differences of the excellent and good rate of postoperative knee joint function and incidence rate of complications between three groups were not statistically significant(P>0.05).Conclusions:The effect of the bilateral LP in treating the coronal fractures of internal condyle in patients with complex tibial plateau fractures is better.
2017, 42(10): 1371-1373.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.024
Abstract:
Objective:To explore the serum levels of differentiated embryonic chondrocyte expression gene 1(DEC1) and liver X receptor β(LXRβ) in Parkinson's disease(PD),and its correlation.Methods:Fifty PD patients and 50 healthy people were divided into the PD group and control group,respectively.The serum levels of DEC1 and LXRβ in two groups were tested.Results:The serum levels of DEC1 and LXRβ in PD group were significantly higher and lower than that in control group,respectively(P<0.01).The differences of serum levels of DEC1 and LXRβ between different genders of PD group were not statistically significant(P>0.05).The serum level of LXRβ in more than or equal to 60 years old PD patients was lower than that in less than 60 years old PD patients(P<0.05).The serum levels of DEC1 and LXRβ in PD patients with cognitive dysfunction were significantly higher and lower than those in PD patients without cognitive dysfunction,respectively(P<0.01).With the deterioration of PD patients,the serum levels of DEC1 and LXRβ gradually increased and decreased,respectively(P<0.05 to P<0.01).The serum level of DEC1 was negatively correlated with the level of LXRβ in PD patients(r=-0.326,P<0.05).Conclusions:The serum level of DEC1 is negatively correlated with the level of LXRβ in PD patients,which may be involved in the onset and progression of PD.
Objective:To explore the serum levels of differentiated embryonic chondrocyte expression gene 1(DEC1) and liver X receptor β(LXRβ) in Parkinson's disease(PD),and its correlation.Methods:Fifty PD patients and 50 healthy people were divided into the PD group and control group,respectively.The serum levels of DEC1 and LXRβ in two groups were tested.Results:The serum levels of DEC1 and LXRβ in PD group were significantly higher and lower than that in control group,respectively(P<0.01).The differences of serum levels of DEC1 and LXRβ between different genders of PD group were not statistically significant(P>0.05).The serum level of LXRβ in more than or equal to 60 years old PD patients was lower than that in less than 60 years old PD patients(P<0.05).The serum levels of DEC1 and LXRβ in PD patients with cognitive dysfunction were significantly higher and lower than those in PD patients without cognitive dysfunction,respectively(P<0.01).With the deterioration of PD patients,the serum levels of DEC1 and LXRβ gradually increased and decreased,respectively(P<0.05 to P<0.01).The serum level of DEC1 was negatively correlated with the level of LXRβ in PD patients(r=-0.326,P<0.05).Conclusions:The serum level of DEC1 is negatively correlated with the level of LXRβ in PD patients,which may be involved in the onset and progression of PD.
2017, 42(10): 1374-1376.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.025
Abstract:
Objective:To explore the clinical effects of artificial dermis stent implantation combined with autologous split-thickness skin graft in repairing the scar contracture in children with limbs burn.Methods:Twenty-eight children with scar contracture after burn were divided into the observation group and control group according to the treatment method(14 cases each group).The observation group was treated with artificial dermis combined with autologous split-thickness skin graft after scar excision,and the control group were treated with thickness skin graft after scar release.The healing time of donor skin area,survival rate of skin graft,wound infection rate after 1 week of operation,and scar hyperplasia in donor skin area and skin graft area between two groups were compared.Results:The healing time of donor skin area in observation group was significantly shorter than that in control group(P<0.01).No infection in two groups was found after 1 week and 2 weeks of operation,and all skin grafts were survival.The difference of the VSS score between two groups after 6 months of following up was not statistically significant(P>0.05),and the score of skin graft area in observation group was better than that in control group(P<0.01).Conclusions:The clinical effect of artificial dermis stent implantation combined with autologous split-thickness skin graft in repairing the scar contracture in children with limbs burn is good,which is worthy of promotion.
Objective:To explore the clinical effects of artificial dermis stent implantation combined with autologous split-thickness skin graft in repairing the scar contracture in children with limbs burn.Methods:Twenty-eight children with scar contracture after burn were divided into the observation group and control group according to the treatment method(14 cases each group).The observation group was treated with artificial dermis combined with autologous split-thickness skin graft after scar excision,and the control group were treated with thickness skin graft after scar release.The healing time of donor skin area,survival rate of skin graft,wound infection rate after 1 week of operation,and scar hyperplasia in donor skin area and skin graft area between two groups were compared.Results:The healing time of donor skin area in observation group was significantly shorter than that in control group(P<0.01).No infection in two groups was found after 1 week and 2 weeks of operation,and all skin grafts were survival.The difference of the VSS score between two groups after 6 months of following up was not statistically significant(P>0.05),and the score of skin graft area in observation group was better than that in control group(P<0.01).Conclusions:The clinical effect of artificial dermis stent implantation combined with autologous split-thickness skin graft in repairing the scar contracture in children with limbs burn is good,which is worthy of promotion.
2017, 42(10): 1377-1379.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.026
Abstract:
Objective:To explore the effects of the patient controlled epidural analgesia with ropivacaine during delivery.Methods:One hundred and forty pregnant women were divided into the group A(57 cases),group B(48 cases) and group C(35 cases) according to the anesthesia method during the delivery.The group A,group B and group C were treated with ropivacaine for patient-controlled epidural analgesia during delivery,bupivacaine for patient-controlled epidural analgesia during delivery and painless labor,respectively.The VAS score at orifice of uterus opening 2 to 3 cm(T1) and full(T2),stress response and lateral episiotomy rate between three groups were compared.Results:The differences of the VAS scores between three groups at T1 were not statistically significant(P>0.05).Compared at T1,the VAS scores in group A and group B at T2 significantly decreased(P<0.01),and which in group C significantly increased(P<0.01).At T2,the VAS score in group A was significantly lower than that in group B and group C,and the VAS score in group B was significantly lower than that in group C(P<0.01).The differences of the levels of blood glucose and Cortisol between three groups at T1 were not statistically significant(P>0.05).Compared at T1,the levels of blood glucose and Cortisol in three groups at T2 increased(P<0.05 to P<0.01).At T2,the levels of blood glucose and Cortisol in group A were lower than those in group B and group C(P<0.01 to P<0.05),and the levels of blood glucose and Cortisol in group B were lower than those in group C(P<0.01 and P<0.05).The lateral episiotomy rate in group C was higher than that in group A and group B(P<0.01),the difference of which between group A and group B was not statistically significant(P>0.05).Conclusions:The effect of patient controlled epidural analgesia with ropivacaine is good,less pain,light emergency response and low lateral episiotomy rate.
Objective:To explore the effects of the patient controlled epidural analgesia with ropivacaine during delivery.Methods:One hundred and forty pregnant women were divided into the group A(57 cases),group B(48 cases) and group C(35 cases) according to the anesthesia method during the delivery.The group A,group B and group C were treated with ropivacaine for patient-controlled epidural analgesia during delivery,bupivacaine for patient-controlled epidural analgesia during delivery and painless labor,respectively.The VAS score at orifice of uterus opening 2 to 3 cm(T1) and full(T2),stress response and lateral episiotomy rate between three groups were compared.Results:The differences of the VAS scores between three groups at T1 were not statistically significant(P>0.05).Compared at T1,the VAS scores in group A and group B at T2 significantly decreased(P<0.01),and which in group C significantly increased(P<0.01).At T2,the VAS score in group A was significantly lower than that in group B and group C,and the VAS score in group B was significantly lower than that in group C(P<0.01).The differences of the levels of blood glucose and Cortisol between three groups at T1 were not statistically significant(P>0.05).Compared at T1,the levels of blood glucose and Cortisol in three groups at T2 increased(P<0.05 to P<0.01).At T2,the levels of blood glucose and Cortisol in group A were lower than those in group B and group C(P<0.01 to P<0.05),and the levels of blood glucose and Cortisol in group B were lower than those in group C(P<0.01 and P<0.05).The lateral episiotomy rate in group C was higher than that in group A and group B(P<0.01),the difference of which between group A and group B was not statistically significant(P>0.05).Conclusions:The effect of patient controlled epidural analgesia with ropivacaine is good,less pain,light emergency response and low lateral episiotomy rate.
2017, 42(10): 1380-1382.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.027
Abstract:
Objective:To evaluate the clinical efficacy of the pelvic floor rehabilitation instrument combined with muscle training in the treatment of female pelvic floor dysfunction(PFD).Methods:One hundred and twenty-four female patients with PFD were randomly divided into the simple training group and joint group(62 cases each group).The simple training group and joint group were treated with the pelvic floor muscle training and pelvic floor rehabilitation instrument combined with muscle training for 4 weeks,respectively.The scores of quality of life in two groups were evaluated after treatment using SCL-90.Results:After treatment,the clinical efficacy in joint group was better than that in simple training group(P<0.01).There was no statistical significance in muscle electrical potential between two groups before treatment(P>0.05).After treatment,the pelvic floor muscle potential in two groups significantly increased compared with before treatment(P<0.01),and the increasing degree of which in joint group was significantly higher than that in simple training group(P<0.01).After treatment,the scores of compulsion,interpersonal relationship,hostility,paranoid and psychoticism in joint group were significantly lower than those in simple training group(P<0.01).Conclusions:Pelvic floor rehabilitation instrument combined with muscle training can effectively improve prognosis,electrophysiological properties of pelvic floor and quality of life of patients with PFD,which is worthy of promotion and application.
Objective:To evaluate the clinical efficacy of the pelvic floor rehabilitation instrument combined with muscle training in the treatment of female pelvic floor dysfunction(PFD).Methods:One hundred and twenty-four female patients with PFD were randomly divided into the simple training group and joint group(62 cases each group).The simple training group and joint group were treated with the pelvic floor muscle training and pelvic floor rehabilitation instrument combined with muscle training for 4 weeks,respectively.The scores of quality of life in two groups were evaluated after treatment using SCL-90.Results:After treatment,the clinical efficacy in joint group was better than that in simple training group(P<0.01).There was no statistical significance in muscle electrical potential between two groups before treatment(P>0.05).After treatment,the pelvic floor muscle potential in two groups significantly increased compared with before treatment(P<0.01),and the increasing degree of which in joint group was significantly higher than that in simple training group(P<0.01).After treatment,the scores of compulsion,interpersonal relationship,hostility,paranoid and psychoticism in joint group were significantly lower than those in simple training group(P<0.01).Conclusions:Pelvic floor rehabilitation instrument combined with muscle training can effectively improve prognosis,electrophysiological properties of pelvic floor and quality of life of patients with PFD,which is worthy of promotion and application.
2017, 42(10): 1383-1385.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.028
Abstract:
Objective:To explore the clinical effects of Ilizarov bone shortening-lengthening in the treatment of the tibia complicated with soft tissue defect.Methods:Ninety-five patients with tibia complicated with soft tissue defect were randomly divided into the observation group(48 cases) and control group(47 cases).The control group was treated with Ilizarov bone transport,and the observation group was treated with Ilizarov bone shortening-lengthening.The operation index and clinical curative effect between two groups were compared.Results:The hospital stay,and healing time of bone defect and wound closure time in observation group were less than those in control group(P<0.01).The clinical total effective rate in observation group(97.9%) was higher than that in control group(83.0%)(P<0.05).The postoperative infection rate in observation group was significantly lower than that in control group(P<0.05).Conclusions:The Ilizarov bone shortening-lengthening in the treatment of the tibia complicated with soft tissue defect has good effect,less complication and short healing time of bone defect,which can quicken the recovery of patients,and worthy of clinical popularization and application.
Objective:To explore the clinical effects of Ilizarov bone shortening-lengthening in the treatment of the tibia complicated with soft tissue defect.Methods:Ninety-five patients with tibia complicated with soft tissue defect were randomly divided into the observation group(48 cases) and control group(47 cases).The control group was treated with Ilizarov bone transport,and the observation group was treated with Ilizarov bone shortening-lengthening.The operation index and clinical curative effect between two groups were compared.Results:The hospital stay,and healing time of bone defect and wound closure time in observation group were less than those in control group(P<0.01).The clinical total effective rate in observation group(97.9%) was higher than that in control group(83.0%)(P<0.05).The postoperative infection rate in observation group was significantly lower than that in control group(P<0.05).Conclusions:The Ilizarov bone shortening-lengthening in the treatment of the tibia complicated with soft tissue defect has good effect,less complication and short healing time of bone defect,which can quicken the recovery of patients,and worthy of clinical popularization and application.
2017, 42(10): 1386-1388.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.029
Abstract:
Objective:To analyze the effects between Jianweixiaoshi oral liquid and intestine-stomach Kang mixture in the treatment of functional dyspepsia of children.Methods:One hundred and twelve children with functional dyspepsia were randomly divided into the observation group and control group using the digital method(56 cases each group).The observation group and control group were treated with Jianweixiaoshi oral liquid and intestine-stomach Kang mixture,respectively.The therapeutic effect and safety between two groups were compared after treatment.Results:The difference of clinical symptom between two groups before treatment was not statistically significant(P>0.05).After treatment,the symptoms in two groups were significantly improved compared before treatment(P<0.01),and the difference of the symptom improvement between two groups was not statistically significant(P>0.05).The difference of the effective rate between two groups was not statistically significant(P>0.05),but the cure rate in observation group was lower than that in control group(P<0.05).After treatment,the number of cases with normal gastric electric rhythm significantly increased(P<0.01),and which in observation group was higher than that in control group(P<0.05).Conclusions:The curative effects of two kinds of medication are good,the cure rate of intestine-stomach in treating the functional dyspepsia of children is better,and the gastric electric rhythm of Jianweixiaoshi oral liquid in treating the functional dyspepsia of children is better,which is worthy of reference in clinic.
Objective:To analyze the effects between Jianweixiaoshi oral liquid and intestine-stomach Kang mixture in the treatment of functional dyspepsia of children.Methods:One hundred and twelve children with functional dyspepsia were randomly divided into the observation group and control group using the digital method(56 cases each group).The observation group and control group were treated with Jianweixiaoshi oral liquid and intestine-stomach Kang mixture,respectively.The therapeutic effect and safety between two groups were compared after treatment.Results:The difference of clinical symptom between two groups before treatment was not statistically significant(P>0.05).After treatment,the symptoms in two groups were significantly improved compared before treatment(P<0.01),and the difference of the symptom improvement between two groups was not statistically significant(P>0.05).The difference of the effective rate between two groups was not statistically significant(P>0.05),but the cure rate in observation group was lower than that in control group(P<0.05).After treatment,the number of cases with normal gastric electric rhythm significantly increased(P<0.01),and which in observation group was higher than that in control group(P<0.05).Conclusions:The curative effects of two kinds of medication are good,the cure rate of intestine-stomach in treating the functional dyspepsia of children is better,and the gastric electric rhythm of Jianweixiaoshi oral liquid in treating the functional dyspepsia of children is better,which is worthy of reference in clinic.
2017, 42(10): 1391-1393,1396.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.031
Abstract:
Objective:To investigate the community health service(CHS) utilization and satisfaction of Bengshan district residents in Bengbu city,and its influencing factors.Methods:The community residents(more than or equal to 15 years old) from Benshang district were investigated using typical sampling method,and the results of which was statistically analyzed using one-way ANOVA.Results:The awareness rate of residents to CHS was 83.60%(311/372),and 156 people(41.94%) utilized the CHS in the latest one year.The evaluation of residents on service attitude of medical staff and protecting personal privacy were the highest,and the evaluation of residents on clinical application verbosity,basic drug supply,drug price,charge,basic equipment configuration,following-up,health examination,health education service and maternal and child care were lower,especially the maternal and child care was the lowest in CHS institutions.The main factors affecting the residents utilizing the CHS included monthly income,medical payment and proportion of family income(P<0.05 toP<0.01),and the main factors affecting satisfaction of residents included age and occupation(P<0.05 to P<0.01).Conclusions:Although the awareness of residents CHS is higher,the utilization and satisfaction of residents on CHS are relatively low because of the shortage of software and hardware of CHS institution and uncorrect clinic concept of residents.Therefore,the relevant departments should take targeted measures to the main influencing factors to develop the "Six in One" function of CHS.
Objective:To investigate the community health service(CHS) utilization and satisfaction of Bengshan district residents in Bengbu city,and its influencing factors.Methods:The community residents(more than or equal to 15 years old) from Benshang district were investigated using typical sampling method,and the results of which was statistically analyzed using one-way ANOVA.Results:The awareness rate of residents to CHS was 83.60%(311/372),and 156 people(41.94%) utilized the CHS in the latest one year.The evaluation of residents on service attitude of medical staff and protecting personal privacy were the highest,and the evaluation of residents on clinical application verbosity,basic drug supply,drug price,charge,basic equipment configuration,following-up,health examination,health education service and maternal and child care were lower,especially the maternal and child care was the lowest in CHS institutions.The main factors affecting the residents utilizing the CHS included monthly income,medical payment and proportion of family income(P<0.05 toP<0.01),and the main factors affecting satisfaction of residents included age and occupation(P<0.05 to P<0.01).Conclusions:Although the awareness of residents CHS is higher,the utilization and satisfaction of residents on CHS are relatively low because of the shortage of software and hardware of CHS institution and uncorrect clinic concept of residents.Therefore,the relevant departments should take targeted measures to the main influencing factors to develop the "Six in One" function of CHS.
2017, 42(10): 1394-1396.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.032
Abstract:
Objective:To investigate the iodine nutritional status of children and pregnant women before and after edible salt iodine decreasing in Bengbu city,and provide the basis for scientific supplementing iodine and preventing iodine deficiency disease.Methods:Eight to ten years old school-age children and pregnant women in Bengbu were investigated before and after edible salt iodine decreasing.The iodine level of morning urine was detected using the WS/T107-2006 urine iodine of arsenic cerium catalytic spectrophotometry method.The iodine content of salt was detected using GB/T13025.7 direct titration method.Results:In 2012 and 2014,the qualified rates of iodine salt in Bengbu were 95.84% and 98.43%,respectively,and the consumption rate of qualified iodine salt were 95.51% and 98.38%,respectively.The urinary iodine median in 8 to 10 years old school-age children and pregnant women before and after iodine decreasing were 307.6 μg/L,219.6 μg/L and 264.5 μg/L,182.9 μg/L in Bengbu,respectively.The urinary iodine median in 8 to 10 years old school-age children and pregnant women after iodine decreasing significantly decreased compared with before iodine decreasing(P<0.01).Conclusions:The urinary iodine level in 8 to 10 years old school-age children decreases after edible salt iodine decreasing,the urinary iodine median still exceeds normal,and the excessive iodine intake in children is found.Before and after edible salt iodine decreasing,the urinary iodine median in pregnant women is normal,and the insufficient iodine intake in pregnant women is found.
Objective:To investigate the iodine nutritional status of children and pregnant women before and after edible salt iodine decreasing in Bengbu city,and provide the basis for scientific supplementing iodine and preventing iodine deficiency disease.Methods:Eight to ten years old school-age children and pregnant women in Bengbu were investigated before and after edible salt iodine decreasing.The iodine level of morning urine was detected using the WS/T107-2006 urine iodine of arsenic cerium catalytic spectrophotometry method.The iodine content of salt was detected using GB/T13025.7 direct titration method.Results:In 2012 and 2014,the qualified rates of iodine salt in Bengbu were 95.84% and 98.43%,respectively,and the consumption rate of qualified iodine salt were 95.51% and 98.38%,respectively.The urinary iodine median in 8 to 10 years old school-age children and pregnant women before and after iodine decreasing were 307.6 μg/L,219.6 μg/L and 264.5 μg/L,182.9 μg/L in Bengbu,respectively.The urinary iodine median in 8 to 10 years old school-age children and pregnant women after iodine decreasing significantly decreased compared with before iodine decreasing(P<0.01).Conclusions:The urinary iodine level in 8 to 10 years old school-age children decreases after edible salt iodine decreasing,the urinary iodine median still exceeds normal,and the excessive iodine intake in children is found.Before and after edible salt iodine decreasing,the urinary iodine median in pregnant women is normal,and the insufficient iodine intake in pregnant women is found.
2017, 42(10): 1397-1399,1403.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.033
Abstract:
Objective:To explore the effects of "5A mode" nursing intervention program on elderly with orthostatic hypotension(OH).Methods:Forty-six elderly OH patients were randomly divided into the control group and intervention group(23 cases each group).The control group were nursed with the standard nursing process,and the intervention group were nursed with "5A mode" nursing intervention process.The awareness rate of OH,frequency of adverse symptoms and nursing satisfaction in two groups were evaluated before intervention and after 6 months of intervention.Results:Before intervention,the differences of the awareness rate of OH and frequency of 7-day adverse symptoms between two groups were not statistically significant(P>0.05).After intervention,the awareness rates of OH in two groups were significantly higher than that before intervention,and which in intervention group was significantly higher than that in control group(P<0.01).After intervention,the frequency of adverse symptoms in two groups were significantly lower than that before intervention,and which in intervention group was significantly lower than that in control group(P<0.01).The score of nursing satisfaction in intervention group was significantly higher than that in control group(P<0.05).Conclusions:The 5A mode nursing intervention program can effectively improve the awareness rate of OH in elderly,reduce the frequency of adverse symptoms and increase the nursing satisfaction.
Objective:To explore the effects of "5A mode" nursing intervention program on elderly with orthostatic hypotension(OH).Methods:Forty-six elderly OH patients were randomly divided into the control group and intervention group(23 cases each group).The control group were nursed with the standard nursing process,and the intervention group were nursed with "5A mode" nursing intervention process.The awareness rate of OH,frequency of adverse symptoms and nursing satisfaction in two groups were evaluated before intervention and after 6 months of intervention.Results:Before intervention,the differences of the awareness rate of OH and frequency of 7-day adverse symptoms between two groups were not statistically significant(P>0.05).After intervention,the awareness rates of OH in two groups were significantly higher than that before intervention,and which in intervention group was significantly higher than that in control group(P<0.01).After intervention,the frequency of adverse symptoms in two groups were significantly lower than that before intervention,and which in intervention group was significantly lower than that in control group(P<0.01).The score of nursing satisfaction in intervention group was significantly higher than that in control group(P<0.05).Conclusions:The 5A mode nursing intervention program can effectively improve the awareness rate of OH in elderly,reduce the frequency of adverse symptoms and increase the nursing satisfaction.
2017, 42(10): 1400-1403.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.034
Abstract:
Objective:To investigate the effects of staging psychological nursing on the negative emotion and quality of life of postoperative patients treated with cardiothoracic surgery.Methods:One hundred and twenty-eight patients treated with cardiothoracic surgery were divided into the observation group(65 cases) and control group(63 cases) using random number table.The control group and observation group were nursed with routine care and staging psychological nursing based on routine care,respectively.The social function,sleep quality,anxiety and depression in two groups between before and after intervention were compared,and the nursing satisfaction degree between two groups was compared.Results:Before nursing,there were no significant differences in the scores of social function,anxiety and depression between two groups(P>0.05).After nursing,the improvement of the social function,anxiety and depression in observation group was significantly better than that in control group(P<0.01).Before nursing,there were no significant differences in the sleep quality,sleep time,sleep efficiency,sleep disorders,hypnotic drug and daytime dysfunction between two groups(P>0.05).After nursing,the improvement of each dimension in observation group was significantly better than that in control group(P<0.01).Conclusions:The application of staging psychological nursing in patients treated with cardiothoracic surgery can effectively alleviate the negative emotion and quality of life in postoperative patients,which has certain clinical reference values.
Objective:To investigate the effects of staging psychological nursing on the negative emotion and quality of life of postoperative patients treated with cardiothoracic surgery.Methods:One hundred and twenty-eight patients treated with cardiothoracic surgery were divided into the observation group(65 cases) and control group(63 cases) using random number table.The control group and observation group were nursed with routine care and staging psychological nursing based on routine care,respectively.The social function,sleep quality,anxiety and depression in two groups between before and after intervention were compared,and the nursing satisfaction degree between two groups was compared.Results:Before nursing,there were no significant differences in the scores of social function,anxiety and depression between two groups(P>0.05).After nursing,the improvement of the social function,anxiety and depression in observation group was significantly better than that in control group(P<0.01).Before nursing,there were no significant differences in the sleep quality,sleep time,sleep efficiency,sleep disorders,hypnotic drug and daytime dysfunction between two groups(P>0.05).After nursing,the improvement of each dimension in observation group was significantly better than that in control group(P<0.01).Conclusions:The application of staging psychological nursing in patients treated with cardiothoracic surgery can effectively alleviate the negative emotion and quality of life in postoperative patients,which has certain clinical reference values.
2017, 42(10): 1404-1407.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.035
Abstract:
Objective:To discuss the influence of the cognitive behavior intervention on the anxiety,depression,treatment compliance and self-efficacy in patients treated with percutaneous coronary intervention(PCI).Methods:Eighty-four patients treated with PCI were randomly divided into the observation group and control group(42 cases each group).The control group was treated with routine nursing,and the observation group was treated with cognitive behavior intervention based on routine nursing.The scores of SAS and SDS before and after operation,and treatment compliance,incidence rate of cardiovascular events and self-efficacy after operation between two groups were compared.Results:The differences of the scores of SAS and SDS before 1 day of operation between two groups were not statistically significant(P>0.05).The scores of SAS and SDS in observation group after 1,3 and 5 days of operation were significantly lower than those in control group(P<0.01),higher than those in observation group before 1 day of operation(P<0.01),and gradually decreased with the time prolonging(P<0.05 to P<0.01).After PCI,the compliance rates of medication,health behavior,regular review,exercise treatment and diet treatment in observation group were better than those in control group(P<0.05).The incidence rate of cardiovascular event in observation group(7.1%) was lower than that in control group(23.8%)(P<0.05).After the intervention,except for the emotion control,the self-efficacy score,and the scores of symptom management,role function and communication ability in observation group were better than those in control group(P<0.01).The cognition of coronary heart disease knowledge was positively correlated with the scores of symptom management,role function and communication ability(P<0.05 to P<0.01),and not obviously correlated with emotion control(P>0.05).Conclusions:Cognitive behavior intervention can improve the levels of anxiety and depression,improve the cognitive level of coronary heart disease knowledge,strengthen the self-efficacy in patients with coronary heart disease,which has important clinical values.
Objective:To discuss the influence of the cognitive behavior intervention on the anxiety,depression,treatment compliance and self-efficacy in patients treated with percutaneous coronary intervention(PCI).Methods:Eighty-four patients treated with PCI were randomly divided into the observation group and control group(42 cases each group).The control group was treated with routine nursing,and the observation group was treated with cognitive behavior intervention based on routine nursing.The scores of SAS and SDS before and after operation,and treatment compliance,incidence rate of cardiovascular events and self-efficacy after operation between two groups were compared.Results:The differences of the scores of SAS and SDS before 1 day of operation between two groups were not statistically significant(P>0.05).The scores of SAS and SDS in observation group after 1,3 and 5 days of operation were significantly lower than those in control group(P<0.01),higher than those in observation group before 1 day of operation(P<0.01),and gradually decreased with the time prolonging(P<0.05 to P<0.01).After PCI,the compliance rates of medication,health behavior,regular review,exercise treatment and diet treatment in observation group were better than those in control group(P<0.05).The incidence rate of cardiovascular event in observation group(7.1%) was lower than that in control group(23.8%)(P<0.05).After the intervention,except for the emotion control,the self-efficacy score,and the scores of symptom management,role function and communication ability in observation group were better than those in control group(P<0.01).The cognition of coronary heart disease knowledge was positively correlated with the scores of symptom management,role function and communication ability(P<0.05 to P<0.01),and not obviously correlated with emotion control(P>0.05).Conclusions:Cognitive behavior intervention can improve the levels of anxiety and depression,improve the cognitive level of coronary heart disease knowledge,strengthen the self-efficacy in patients with coronary heart disease,which has important clinical values.
2017, 42(10): 1408-1410,1414.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.036
Abstract:
Objective:To investigate the effects of different positions on the ICP,CPP and vital signs in patients with cerebral hemorrhage.Methods:The ICP,CPP,cerebral tissue oxygen saturation(rSO2) and vital signs in 46 hypertension patients with cerebral hemorrhage were monitored at 0°,15°,30° and 45° of bed head.Results:With the elevating of bed head angle,the ICP and CPP value of patients gradually decreased,and the difference of those in different groups were statistically significant(P<0.01 and P<0.05).The results of pairwise comparison showed that the ICP value at 15°,30° and 45° of bed head significantly decreased compared with the 0° of bed head,the ICP value at 30° and 45° of bed head was lower than that at 15° of bed head(P<0.05),and the CPP value at 45° of bed head was higher than that at 0° of bed head(P<0.05).The differences of the rSO2,pulse oxygen saturation,heart rate,systolic pressure,diastolic pressure,mean arterial pressure and body temperature between different angles were not statistically significant(P>0.05).At different angles of bed head,the rSO2 was positively correlated with mean arterial pressure and ICP(P<0.05),and negatively correlated with the CPP(P<0.05).Conclusions:Within the 15° to 30° of bed head,the nursing effect of patients is the best.
Objective:To investigate the effects of different positions on the ICP,CPP and vital signs in patients with cerebral hemorrhage.Methods:The ICP,CPP,cerebral tissue oxygen saturation(rSO2) and vital signs in 46 hypertension patients with cerebral hemorrhage were monitored at 0°,15°,30° and 45° of bed head.Results:With the elevating of bed head angle,the ICP and CPP value of patients gradually decreased,and the difference of those in different groups were statistically significant(P<0.01 and P<0.05).The results of pairwise comparison showed that the ICP value at 15°,30° and 45° of bed head significantly decreased compared with the 0° of bed head,the ICP value at 30° and 45° of bed head was lower than that at 15° of bed head(P<0.05),and the CPP value at 45° of bed head was higher than that at 0° of bed head(P<0.05).The differences of the rSO2,pulse oxygen saturation,heart rate,systolic pressure,diastolic pressure,mean arterial pressure and body temperature between different angles were not statistically significant(P>0.05).At different angles of bed head,the rSO2 was positively correlated with mean arterial pressure and ICP(P<0.05),and negatively correlated with the CPP(P<0.05).Conclusions:Within the 15° to 30° of bed head,the nursing effect of patients is the best.
2017, 42(10): 1411-1414.
doi: 10.13898/j.cnki.issn.1000-2200.2017.10.037
Abstract:
Objective:To investigate the application effects of refinement process management in alleviating the tension of the operating room patients.Methods:January 2014 was set as the interval point time in the general surgery operating room,the patients before 6 months of refinement process management and after 6 months of refinement process management were divided into the control group and observation group,respectively.The control group was coped with the conventional management,and the observation group was coped with the refinement process management(including the personnel management,environment management,material management and process management).The care quality,prognosis,tension and satisfaction in two groups were observed.Results:The nursing qualification rate and care instruments passing rate in observation group were significantly higher than those in control group(P<0.05),and the incidence rates of the accident and nursing complication in observation group were significantly lower than those in control group(P<0.05).The surgical total effective rate in observation group(98.6%) was higher than that in control group(90.0%)(P<0.05).The scores of postoperative 7 d tension in two groups were higher than those of before operation(P<0.05).The nursing satisfaction rate in observation group(100.0%) was higher than that in control group(88.6%)(P<0.05).Conclusions:The application of refinement process management can relieve the tension of patients,and improve the quality of care,operation efficiency and patient`s care evaluation,which is worthy of further promotion.
Objective:To investigate the application effects of refinement process management in alleviating the tension of the operating room patients.Methods:January 2014 was set as the interval point time in the general surgery operating room,the patients before 6 months of refinement process management and after 6 months of refinement process management were divided into the control group and observation group,respectively.The control group was coped with the conventional management,and the observation group was coped with the refinement process management(including the personnel management,environment management,material management and process management).The care quality,prognosis,tension and satisfaction in two groups were observed.Results:The nursing qualification rate and care instruments passing rate in observation group were significantly higher than those in control group(P<0.05),and the incidence rates of the accident and nursing complication in observation group were significantly lower than those in control group(P<0.05).The surgical total effective rate in observation group(98.6%) was higher than that in control group(90.0%)(P<0.05).The scores of postoperative 7 d tension in two groups were higher than those of before operation(P<0.05).The nursing satisfaction rate in observation group(100.0%) was higher than that in control group(88.6%)(P<0.05).Conclusions:The application of refinement process management can relieve the tension of patients,and improve the quality of care,operation efficiency and patient`s care evaluation,which is worthy of further promotion.
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