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2017 Vol. 42, No. 11
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2017, 42(11): 1433-1436.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.001
Abstract:
Objective:To explore the application value of serum level of ubiquitin carboxyl-terminal esterase L1(UCH-L1) in the diagnosis of hypoxic-ischemic encephalopathy in newborn rats.Methods:One hundred and sixty eighty newborn SD rats were randomly divided into the control group,sham-operation group and hypoxic-ischemic brain damage(HIBD) model group(56 rats each group).Each group was randomly subdivided into the 6 h,12 h,24 h,48 h,72 h,96 h and 7 d groups(8 rats each subgroup).The HIBD model was established using the improved Rice method.All rats were sacrificed to obtain blood at different time points.The serum levels of UCH-L1 were tested by ELISA.Results:At different time-points,the differences of the levels of UCH-L1 between three groups were statistically significant(P<0.01),the levels of UCH-L1 in HIBD model group were significantly higher than that in control group and sham-operation group(P<0.01),and the difference between the control group and sham-operation group was not statistically significant(P>0.05).The differences of the levels of UCH-L1 in HIBD model group between different time-points were statistically significant(P<0.01).In HIBD model group,the serum levels of UCH-L1 began to significantly increase at 6 h,reached the peak value at 48 h,then decreased gradually.The more severe the brain damaged,the higher the level of serum UCH-L1 was.Conclusions:The levels of UCH-L1 is positively correlated with the severity of the brain damage.The expression of UCH-L1 can be used an indicator in evaluating the brain damage.
Objective:To explore the application value of serum level of ubiquitin carboxyl-terminal esterase L1(UCH-L1) in the diagnosis of hypoxic-ischemic encephalopathy in newborn rats.Methods:One hundred and sixty eighty newborn SD rats were randomly divided into the control group,sham-operation group and hypoxic-ischemic brain damage(HIBD) model group(56 rats each group).Each group was randomly subdivided into the 6 h,12 h,24 h,48 h,72 h,96 h and 7 d groups(8 rats each subgroup).The HIBD model was established using the improved Rice method.All rats were sacrificed to obtain blood at different time points.The serum levels of UCH-L1 were tested by ELISA.Results:At different time-points,the differences of the levels of UCH-L1 between three groups were statistically significant(P<0.01),the levels of UCH-L1 in HIBD model group were significantly higher than that in control group and sham-operation group(P<0.01),and the difference between the control group and sham-operation group was not statistically significant(P>0.05).The differences of the levels of UCH-L1 in HIBD model group between different time-points were statistically significant(P<0.01).In HIBD model group,the serum levels of UCH-L1 began to significantly increase at 6 h,reached the peak value at 48 h,then decreased gradually.The more severe the brain damaged,the higher the level of serum UCH-L1 was.Conclusions:The levels of UCH-L1 is positively correlated with the severity of the brain damage.The expression of UCH-L1 can be used an indicator in evaluating the brain damage.
2017, 42(11): 1436-1438.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.002
Abstract:
Objective:To observe the effects of Staphylococcus aureus L-form on the growth of human cervical cancer cells(HeLa) in vitro.Methods:The HeLa cells were infected with Staphylococcus aureus L-form by routine method.The adhesion and invasion of Staphylococcus aureus L-form were detected using light and electron microscopy,and the proliferation of HeLa cells was detected using improved MTT assay.Results:The results of light and electron microscopy L-form showed that the Staphylococcus aureus L-form could adhere and invade into HeLa cells.The result of improved MTT method showed that Staphylococcus aureus L-form could promote the proliferation of HeLa cells.Conclusions:Staphylococcus aureus L-form can infect and promote the growth of HeLa cells,which reveals that Staphylococcus aureus L-form may be one of inducting factor of cervical cancer.
Objective:To observe the effects of Staphylococcus aureus L-form on the growth of human cervical cancer cells(HeLa) in vitro.Methods:The HeLa cells were infected with Staphylococcus aureus L-form by routine method.The adhesion and invasion of Staphylococcus aureus L-form were detected using light and electron microscopy,and the proliferation of HeLa cells was detected using improved MTT assay.Results:The results of light and electron microscopy L-form showed that the Staphylococcus aureus L-form could adhere and invade into HeLa cells.The result of improved MTT method showed that Staphylococcus aureus L-form could promote the proliferation of HeLa cells.Conclusions:Staphylococcus aureus L-form can infect and promote the growth of HeLa cells,which reveals that Staphylococcus aureus L-form may be one of inducting factor of cervical cancer.
2017, 42(11): 1439-1442.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.003
Abstract:
Objective:To establish an HPLC method for the content determination of gambogenic acid in tumor cells.Methods:The gambogenic acid in cells was measured using HPLC.The chromatographic column was COSMOSIL C18 column,the mobile phase was 90% methanol:0.1% aqueous phosphoric acid(90:10,V:V),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 360 nm,and the injection volume was 20 μL.Results:The linear relationship between the 0.05 to 30 μg/mL of gambogenic acid and peak area was good(P<0.01).The precision and stability of RSD was lower than 10%.The accuracy of the measure value was less than 10%,the specificity was good.The repeatability of sample determination at the same time and concentration of gambogenic acid was good.Conclusions:The method is simple,convenient and accurate,which is suit for the determination of gambogenic acid in tumor cells.
Objective:To establish an HPLC method for the content determination of gambogenic acid in tumor cells.Methods:The gambogenic acid in cells was measured using HPLC.The chromatographic column was COSMOSIL C18 column,the mobile phase was 90% methanol:0.1% aqueous phosphoric acid(90:10,V:V),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 360 nm,and the injection volume was 20 μL.Results:The linear relationship between the 0.05 to 30 μg/mL of gambogenic acid and peak area was good(P<0.01).The precision and stability of RSD was lower than 10%.The accuracy of the measure value was less than 10%,the specificity was good.The repeatability of sample determination at the same time and concentration of gambogenic acid was good.Conclusions:The method is simple,convenient and accurate,which is suit for the determination of gambogenic acid in tumor cells.
2017, 42(11): 1442-1445.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.004
Abstract:
Objective:To investigate the effects of glycolytic inhibitor 3-bromopyruvate(3-BP) on the proliferation and apoptosis in cisplatin-resistance nasopharyngeal carcinoma cells.Methods:The proliferation-inhibitory effect of 3-BP on HNE1/DDP cells was measured by MTT assay and colony formation assay,and the effect of 3-BP on the apoptosis of HNE1/DDP cells was detected by PI staining.The expression levels of poly ADP-ribose polymerase(PARP,apoptosis-related protein),myeloid cell leukemia-1(Mcl-1),B cell lymphoma/leukemia-2(Bcl-2) were analyzed using Western blotting.Results:3-BP could significantly inhibit the proliferation of HNE1/DDP cells,the IC50 value of 48 h was 260.2 μmol/L,and the low concentrations(10,20,40 μmol/L) 3-BP could obviously inhibit the colony formation of HNE1/DDP cells.The 3-BP could significantly induce the apoptosis of HNE1/DDP cells,and the apoptosis rates of cells treated with 80,160,and 320 μmol/L of 3-BP for 48 h were (13.7±2.1)%,(25.5±2.4)% and(45.5±3.5)%,respectively,and the apoptosis rate of cells in control group was(1.6±0.6)%.The results of Western blotting indicated that the HNE1/DDP cells treated with 3-BP could promote the cleavage of PARP,and downregulate the expression levels of the antiapoptotic proteins Mcl-1 and Bcl-2.Conclusions:3-BP can inhibit the proliferation and induce the apoptosis of HNE1/DDP cells,the mechanism of which may be correlated with the downregulation of antiapoptotic proteins Mcl-1 and Bcl-2.
Objective:To investigate the effects of glycolytic inhibitor 3-bromopyruvate(3-BP) on the proliferation and apoptosis in cisplatin-resistance nasopharyngeal carcinoma cells.Methods:The proliferation-inhibitory effect of 3-BP on HNE1/DDP cells was measured by MTT assay and colony formation assay,and the effect of 3-BP on the apoptosis of HNE1/DDP cells was detected by PI staining.The expression levels of poly ADP-ribose polymerase(PARP,apoptosis-related protein),myeloid cell leukemia-1(Mcl-1),B cell lymphoma/leukemia-2(Bcl-2) were analyzed using Western blotting.Results:3-BP could significantly inhibit the proliferation of HNE1/DDP cells,the IC50 value of 48 h was 260.2 μmol/L,and the low concentrations(10,20,40 μmol/L) 3-BP could obviously inhibit the colony formation of HNE1/DDP cells.The 3-BP could significantly induce the apoptosis of HNE1/DDP cells,and the apoptosis rates of cells treated with 80,160,and 320 μmol/L of 3-BP for 48 h were (13.7±2.1)%,(25.5±2.4)% and(45.5±3.5)%,respectively,and the apoptosis rate of cells in control group was(1.6±0.6)%.The results of Western blotting indicated that the HNE1/DDP cells treated with 3-BP could promote the cleavage of PARP,and downregulate the expression levels of the antiapoptotic proteins Mcl-1 and Bcl-2.Conclusions:3-BP can inhibit the proliferation and induce the apoptosis of HNE1/DDP cells,the mechanism of which may be correlated with the downregulation of antiapoptotic proteins Mcl-1 and Bcl-2.
2017, 42(11): 1446-1449.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.005
Abstract:
Objective:To investigate the correlation between the polymorphisms of CYP2E1 gene single nucleotide rs3813867 and genetic susceptibility of gastric cancer in Hakkaness.Methods:The genotypes and alleles of CYP2E1 rs381386 in 51 gastric cancer patients(gastric cancer group) and 52 healthy people(control group) from Hakkaness in Meizhou area were detected,and the correlation between which and susceptibility of gastric cancer were evaluated.Results:The GG,GC and CC polymorphisms in CYP2E1 rs381386 in Hakkaness in Meizhou were found.The distribution frequency of GG,GC and CC genotypes in gastric cancer group was 62.75%,33.33% and 3.92%,respectively,and that in control group was 59.62%,34.61% and 5.77%,respectively.The distribution frequency of alleles of G and C in gastric cancer group and control group was 79.41% & 20.59% and 76.92% & 23.08%,respectively,and the differences of which between two groups were not statistically significant(P>0.05).The differences of the genotype distribution frequency in different sex and ≥ 60 years old patients between two groups were not statistically significant(P>0.05),and the difference of which in <60 years old patients between two groups was statistically significant(P<0.05).Conclusions:The genetic polymorphisms of CYP2E1 rs3813867 is not significantly correlated with the susceptibility of gastric cancer in Hakkaness.
Objective:To investigate the correlation between the polymorphisms of CYP2E1 gene single nucleotide rs3813867 and genetic susceptibility of gastric cancer in Hakkaness.Methods:The genotypes and alleles of CYP2E1 rs381386 in 51 gastric cancer patients(gastric cancer group) and 52 healthy people(control group) from Hakkaness in Meizhou area were detected,and the correlation between which and susceptibility of gastric cancer were evaluated.Results:The GG,GC and CC polymorphisms in CYP2E1 rs381386 in Hakkaness in Meizhou were found.The distribution frequency of GG,GC and CC genotypes in gastric cancer group was 62.75%,33.33% and 3.92%,respectively,and that in control group was 59.62%,34.61% and 5.77%,respectively.The distribution frequency of alleles of G and C in gastric cancer group and control group was 79.41% & 20.59% and 76.92% & 23.08%,respectively,and the differences of which between two groups were not statistically significant(P>0.05).The differences of the genotype distribution frequency in different sex and ≥ 60 years old patients between two groups were not statistically significant(P>0.05),and the difference of which in <60 years old patients between two groups was statistically significant(P<0.05).Conclusions:The genetic polymorphisms of CYP2E1 rs3813867 is not significantly correlated with the susceptibility of gastric cancer in Hakkaness.
2017, 42(11): 1449-1452.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.006
Abstract:
Objective:To analyze the correlation between subclinical thyroid disease and missed abortion for reducing the missed abortion rate.Methods:Seventy-one patients with missed abortion and 80 normal pregnant women were divided into the observation group and control group,respectively.The serum levels of free triiodothyronine(FT3),free thyroxine(FT4),thyroid stimulating hormone(TSH),thyroglobulin antibody(TGAb) and thyroid microsomal antibody(TMAb) between two groups were compared.The pregnant outcome in two groups was followed up.Results:There was no statistical difference in the serum levels of FT3,FT4,TSH,TGAb and TMAb between two groups(P>0.05).The incidence rate of subclinical thyroid disease in observation group was higher than that in control group(P<0.01),the subclinical hypothyroidism(TSH elevated type) was the main type.The differences of thyroid autoantibody and incidence of hyperthyroidism between two groups were not statistically significant(P>0.05).Conclusions:The subclinical hypothyroidism(TSH elevated type) is correlated with the missed abortion rate,the subclinical hyperthyroidism and thyroid autoantibody are not significantly related to the missed abortion.The impact of subclinical thyroid disease on the missed abortion mainly happens on early pregnancy.
Objective:To analyze the correlation between subclinical thyroid disease and missed abortion for reducing the missed abortion rate.Methods:Seventy-one patients with missed abortion and 80 normal pregnant women were divided into the observation group and control group,respectively.The serum levels of free triiodothyronine(FT3),free thyroxine(FT4),thyroid stimulating hormone(TSH),thyroglobulin antibody(TGAb) and thyroid microsomal antibody(TMAb) between two groups were compared.The pregnant outcome in two groups was followed up.Results:There was no statistical difference in the serum levels of FT3,FT4,TSH,TGAb and TMAb between two groups(P>0.05).The incidence rate of subclinical thyroid disease in observation group was higher than that in control group(P<0.01),the subclinical hypothyroidism(TSH elevated type) was the main type.The differences of thyroid autoantibody and incidence of hyperthyroidism between two groups were not statistically significant(P>0.05).Conclusions:The subclinical hypothyroidism(TSH elevated type) is correlated with the missed abortion rate,the subclinical hyperthyroidism and thyroid autoantibody are not significantly related to the missed abortion.The impact of subclinical thyroid disease on the missed abortion mainly happens on early pregnancy.
2017, 42(11): 1453-1455,1461.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.007
Abstract:
Objective:To investigate the Epstein-Barr virus(EBV) infection condition in hospitalized children from kunming city of Yunnan province,and related clinical popular distribution characteristics of disease spectrum.Methods:The clinical data of children suspected with EBV infection,the EBV nucleic acid positive cases identified using PCR fluorescence method,were retrospectively analyzed.Results:Among 547 blood samples,97 cases with positive EBV nucleic acid were detected,the detection rate of which was 17.73%,which included 56 males(57.73%) and 41 females(42.27%).The mean age of all cases was(4.60±2.84) years,and the pre-school children(0 to 6 years old) in 76 cases(78.35%) were found.The clinical symptoms of children infected with EBV was various.The upper respiratory tract infection symptoms(including fever,cough and lymph node enlargement) were the first symptoms of the EB V infection,and the pneumonia,bronchitis,infectious mononucleosis and pharyngitis were the clinical manifestations,which was accompanied by liver and spleen enlargement,rash,liver and heart muscle function damage.The combined pathogen infection in 71 cases were identified,which included mycoplasma pneumoniae infection in 19 cases(19.59%),typhoid and paratyphoid infection in 18 cases(18.56%),Japanese encephalitis virus infection in 17 cases(17.53%),parainfluenza infection in 8 cases(8.25%),intestinal virus infection in 2 cases(2.06%),other viruses in 7 cases(7.22%) and co-infection in 47 cases(42.27%).The difference of the detection rate in different seasons was not statstically significant(P>0.05).Conclusions:EBV infection is one of the most important pathogen of respiratory tract infection in hospitalized children in Kunming region,the disaese can occure in each season,and the mixture infection with other pathogen is common.
Objective:To investigate the Epstein-Barr virus(EBV) infection condition in hospitalized children from kunming city of Yunnan province,and related clinical popular distribution characteristics of disease spectrum.Methods:The clinical data of children suspected with EBV infection,the EBV nucleic acid positive cases identified using PCR fluorescence method,were retrospectively analyzed.Results:Among 547 blood samples,97 cases with positive EBV nucleic acid were detected,the detection rate of which was 17.73%,which included 56 males(57.73%) and 41 females(42.27%).The mean age of all cases was(4.60±2.84) years,and the pre-school children(0 to 6 years old) in 76 cases(78.35%) were found.The clinical symptoms of children infected with EBV was various.The upper respiratory tract infection symptoms(including fever,cough and lymph node enlargement) were the first symptoms of the EB V infection,and the pneumonia,bronchitis,infectious mononucleosis and pharyngitis were the clinical manifestations,which was accompanied by liver and spleen enlargement,rash,liver and heart muscle function damage.The combined pathogen infection in 71 cases were identified,which included mycoplasma pneumoniae infection in 19 cases(19.59%),typhoid and paratyphoid infection in 18 cases(18.56%),Japanese encephalitis virus infection in 17 cases(17.53%),parainfluenza infection in 8 cases(8.25%),intestinal virus infection in 2 cases(2.06%),other viruses in 7 cases(7.22%) and co-infection in 47 cases(42.27%).The difference of the detection rate in different seasons was not statstically significant(P>0.05).Conclusions:EBV infection is one of the most important pathogen of respiratory tract infection in hospitalized children in Kunming region,the disaese can occure in each season,and the mixture infection with other pathogen is common.
2017, 42(11): 1456-1461.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.008
Abstract:
Objective:To investigate the expressions of Smo and Gli1 in gastric cancer tissues and paracarcinoma tissues,and their correlations with clinicopathological features of gastric cancer.Methods:The protein expressions of Smo and Glil in 75 cases of gastric cancer tissues and 45 cases of paracarcinoma tissues were detected using immunohistochemical EliVision method.The mRNA expressions of Smo and Glil in 30 cases of fresh gastric cancer tissues and paracarcinoma tissues(5 cm distance from cut edge) were detected using RT-PCR technology.Results:The results of immunostochemical staining showed that the positive rates of the protein expressions of Smo and Glil in gastric cancer tissues were significantly higher than those in paracarcinoma tissues(P<0.01).Compared with the paracarcinoma tissues,the results of RT-PCR showed that the mRNA expression levels of Smo and Gli1 significantly increased in gastric cancer tissues(P<0.01).Both methods demonstrated that the differences of the positive expression rates of Smo and Gli1 in different age,gender,tumor differentiation and size were not statistically significant(P>0.05),and the differences of the expression positive rates of Smo and Gli1 in different TNM stage,invasion depth and lymph node metastasis were statistically significant(P<0.01).Conclusions:The protein expressions of Smo and Gli1 are related to the lymph node metastasis,clinical stage and invasion depth of gastric cancer,which hints that Smo and Gli1 may be related to the malignant biological behavior of gastric cancer.The expression of Smo is positively correlated with Glil in gastric cancer,which maybe play a synergistic action in the occurrence and development of gastric cancer.
Objective:To investigate the expressions of Smo and Gli1 in gastric cancer tissues and paracarcinoma tissues,and their correlations with clinicopathological features of gastric cancer.Methods:The protein expressions of Smo and Glil in 75 cases of gastric cancer tissues and 45 cases of paracarcinoma tissues were detected using immunohistochemical EliVision method.The mRNA expressions of Smo and Glil in 30 cases of fresh gastric cancer tissues and paracarcinoma tissues(5 cm distance from cut edge) were detected using RT-PCR technology.Results:The results of immunostochemical staining showed that the positive rates of the protein expressions of Smo and Glil in gastric cancer tissues were significantly higher than those in paracarcinoma tissues(P<0.01).Compared with the paracarcinoma tissues,the results of RT-PCR showed that the mRNA expression levels of Smo and Gli1 significantly increased in gastric cancer tissues(P<0.01).Both methods demonstrated that the differences of the positive expression rates of Smo and Gli1 in different age,gender,tumor differentiation and size were not statistically significant(P>0.05),and the differences of the expression positive rates of Smo and Gli1 in different TNM stage,invasion depth and lymph node metastasis were statistically significant(P<0.01).Conclusions:The protein expressions of Smo and Gli1 are related to the lymph node metastasis,clinical stage and invasion depth of gastric cancer,which hints that Smo and Gli1 may be related to the malignant biological behavior of gastric cancer.The expression of Smo is positively correlated with Glil in gastric cancer,which maybe play a synergistic action in the occurrence and development of gastric cancer.
2017, 42(11): 1462-1464.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.009
Abstract:
Objective:To explore the correlation between iron metabolism and coronary artery stenosis in patients with coronary heart disease(CHD).Methods:Ninety-four CHD patients and 94 non-CHD patients were divided into the observation group and control group,respectively.The serum ferritin(SF) level and total iron binding capacity(TIBC) and ratio of SF/TIBC between two groups were compared.Results:The SF level and ratio of SF/TIBC in observation group were significantly higher than those in control group,and the TIBC in observation group was significantly lower than that in control group(P<0.01).The differences of the SF level,TIBC and ratio of SF/TIBC between mild stenosis group,moderate stenosis group and severe stenosis group were statistically significant(P<0.01).The results of pairwise comparison showed that the SF level and ratio of SF/TIBC in severe stenosis group were higher than those in mild stenosis group and moderate stenosis group,the TIBC in severe stenosis group was lower than that in other two groups(P<0.05 and P<0.01).The ratio of SF/TIBC in moderate stenosis group was higher than that in mild stenosis group,and the TIBC in moderate stenosis group was significantly lower than that in mild stenosis group(P<0.01).The Gensini score in CHD patients was significantly positive correlated with SF level and ratio of SF/TIBC(r=0.419,r=0.624,P<0.05),and significantly negatively correlated with TIBC(r=-0.347,P<0.05).Conclusions:Iron overload is closely associated with coronary artery stenosis in CHD patients.The iron metabolism indexes should be regularly detected in CHD patients.
Objective:To explore the correlation between iron metabolism and coronary artery stenosis in patients with coronary heart disease(CHD).Methods:Ninety-four CHD patients and 94 non-CHD patients were divided into the observation group and control group,respectively.The serum ferritin(SF) level and total iron binding capacity(TIBC) and ratio of SF/TIBC between two groups were compared.Results:The SF level and ratio of SF/TIBC in observation group were significantly higher than those in control group,and the TIBC in observation group was significantly lower than that in control group(P<0.01).The differences of the SF level,TIBC and ratio of SF/TIBC between mild stenosis group,moderate stenosis group and severe stenosis group were statistically significant(P<0.01).The results of pairwise comparison showed that the SF level and ratio of SF/TIBC in severe stenosis group were higher than those in mild stenosis group and moderate stenosis group,the TIBC in severe stenosis group was lower than that in other two groups(P<0.05 and P<0.01).The ratio of SF/TIBC in moderate stenosis group was higher than that in mild stenosis group,and the TIBC in moderate stenosis group was significantly lower than that in mild stenosis group(P<0.01).The Gensini score in CHD patients was significantly positive correlated with SF level and ratio of SF/TIBC(r=0.419,r=0.624,P<0.05),and significantly negatively correlated with TIBC(r=-0.347,P<0.05).Conclusions:Iron overload is closely associated with coronary artery stenosis in CHD patients.The iron metabolism indexes should be regularly detected in CHD patients.
2017, 42(11): 1465-1468.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.010
Abstract:
Objective:To investigate the expression levels of midkine(MK) and p63α in cervical squamous cell carcinoma(SCC) tissue,and analyze its significance in the development of cervical SCC.Methods:Eighty-nine cervical SCC patients and 50 leiomyoma or adenomyosis patients treated with hysterectomy were divided into the SCC group and control group,respectively.The expression levels of MK and p63α in two groups were detected using immunohistochemical SP method.The clinical pathological feature in SCC group was collected,and the 5-year survival in SCC group was followed up by telephone or outpatient review.The levels of MK and p63α in cervical tissues between two groups were compared.The correlation between two proteins was analyzed using Spearman rank correlation test.The effects of MK and p63α on the clinicopathological features and 5-year survival in SCC group were investigated.Results:The high expression rate of MK(29.21%) and low expression rate of MK(39.33%) in SCC group were significantly higher than that in control group(2.00% and 12.00%) (P<0.01),the positive expression rate of p63α in SCC group(68.54%) was significantly lower than that in control group(90.00%) (P<0.01),and the expression of MK was negatively correlated with p63α(P<0.05).The differences of the expression levels of MK and p63α in different age,tumor size,differentiation degree and 5-year survival in SCC group were statistically significant(P<0.05 to P<0.01).The expression rates of MK and p63α in patients with age ≥ 49 years old,tumor size>4 cm and low differentiation were significantly higher and lower than those in other features patients in SCC group,respectively(P<0.05 to P<0.01).The expression rates of MK and p63α in survival patients were significantly lower and higher than those in death patients,respectively(P<0.05 to P<0.01).Conclusions:High expression level of MK and low expression level of p63α can be involved in the pathogenesis and development of cervical SCC,and affect the degree of malignancy and prognosis.
Objective:To investigate the expression levels of midkine(MK) and p63α in cervical squamous cell carcinoma(SCC) tissue,and analyze its significance in the development of cervical SCC.Methods:Eighty-nine cervical SCC patients and 50 leiomyoma or adenomyosis patients treated with hysterectomy were divided into the SCC group and control group,respectively.The expression levels of MK and p63α in two groups were detected using immunohistochemical SP method.The clinical pathological feature in SCC group was collected,and the 5-year survival in SCC group was followed up by telephone or outpatient review.The levels of MK and p63α in cervical tissues between two groups were compared.The correlation between two proteins was analyzed using Spearman rank correlation test.The effects of MK and p63α on the clinicopathological features and 5-year survival in SCC group were investigated.Results:The high expression rate of MK(29.21%) and low expression rate of MK(39.33%) in SCC group were significantly higher than that in control group(2.00% and 12.00%) (P<0.01),the positive expression rate of p63α in SCC group(68.54%) was significantly lower than that in control group(90.00%) (P<0.01),and the expression of MK was negatively correlated with p63α(P<0.05).The differences of the expression levels of MK and p63α in different age,tumor size,differentiation degree and 5-year survival in SCC group were statistically significant(P<0.05 to P<0.01).The expression rates of MK and p63α in patients with age ≥ 49 years old,tumor size>4 cm and low differentiation were significantly higher and lower than those in other features patients in SCC group,respectively(P<0.05 to P<0.01).The expression rates of MK and p63α in survival patients were significantly lower and higher than those in death patients,respectively(P<0.05 to P<0.01).Conclusions:High expression level of MK and low expression level of p63α can be involved in the pathogenesis and development of cervical SCC,and affect the degree of malignancy and prognosis.
2017, 42(11): 1469-1471,1475.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.011
Abstract:
Objective:To explore the role of DAN2 expression in breast cancer maliganant degree and positive estrogen receptor(ER).Methods:The DNA2 mRNA in carcinoma tissue and para-carcinoma tissue of 132 breast cancer patients were extracted,and the expression level of DNA2 mRNA was detected using real-time quantitative PCR.The relationships between ER,and DNA2 expression and degree of malignancy were analyzed.The expressions of DNA2 protein in ER positive and ER negative cells were detected,which was used to verify the positive correlation between DNA2 and ER.Results:The positive ER was positive correlation with DNA2 expression,and the positive ER could up-regulate the DNA2 expression.The positive ER was not correlation with tumor malignance,and the positive ER upregulated the DNA2 expression to increase the MCF7 cells resistance to CPT.Conclusions:The regulation of the DNA2 expression can significantly promote the metastasis of malignant tumor,and the positive ER up-regulates the DNA2 expression to increase the drug resistance of tumor cells.
Objective:To explore the role of DAN2 expression in breast cancer maliganant degree and positive estrogen receptor(ER).Methods:The DNA2 mRNA in carcinoma tissue and para-carcinoma tissue of 132 breast cancer patients were extracted,and the expression level of DNA2 mRNA was detected using real-time quantitative PCR.The relationships between ER,and DNA2 expression and degree of malignancy were analyzed.The expressions of DNA2 protein in ER positive and ER negative cells were detected,which was used to verify the positive correlation between DNA2 and ER.Results:The positive ER was positive correlation with DNA2 expression,and the positive ER could up-regulate the DNA2 expression.The positive ER was not correlation with tumor malignance,and the positive ER upregulated the DNA2 expression to increase the MCF7 cells resistance to CPT.Conclusions:The regulation of the DNA2 expression can significantly promote the metastasis of malignant tumor,and the positive ER up-regulates the DNA2 expression to increase the drug resistance of tumor cells.
2017, 42(11): 1472-1475.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.012
Abstract:
Objective:To explore the effects of Yindan Xinnaotong soft capsule on the oxidative stress and vascular endothelial function in patients with type 2 diabetes mellitus complicated with coronary heart disease(CHD).Methods:A total of 150 patients with T2DM complicated with CHD were randomly divided into the observation group and the control group.The control group was treated with reducing blood glucose,reducing lipid,anti-coagulation,β-receptor blockers,nitrates and angiotensin converting enzyme inhibitor,and the observation group was additionally treated with Yindan Xinnaotong soft capsule by oral based on the control group for 12 weeks.The effects in two groups were evaluated after 12 weeks of treatment.Results:The levels of whole blood viscosity(high shear,middle shear and low shear),hematokrit,plasma viscosity and fibrinogen in observation group were significantly lower than those in control group(P<0.01).The levels of superoxide dismutase and malondialdehyde in observation group were significantly higher and lower than those in control group,respectively(P<0.01).The levels of endothelin,and the level of NO,non-endothelium-dependent vasodilation function induced by nitroglycerin and endothelium-dependent vasodilation function induced by blood flow in observation group were significantly lower and higher than those in control group,respectively(P<0.01).After treatment,the angina frequency,angina duration and nitroglycerin dosage in observation group were significantly lower than those in control group(P<0.01).Conclusions:Yindan Xinnaotong soft capsule in the treatment of T2DM complicated with CHD can effectively improve the hemorheology,alleviate the oxidative stress state,protect the vascular endothelial function and relieve the clinical symptoms,which is worthy of promotion in clinic.
Objective:To explore the effects of Yindan Xinnaotong soft capsule on the oxidative stress and vascular endothelial function in patients with type 2 diabetes mellitus complicated with coronary heart disease(CHD).Methods:A total of 150 patients with T2DM complicated with CHD were randomly divided into the observation group and the control group.The control group was treated with reducing blood glucose,reducing lipid,anti-coagulation,β-receptor blockers,nitrates and angiotensin converting enzyme inhibitor,and the observation group was additionally treated with Yindan Xinnaotong soft capsule by oral based on the control group for 12 weeks.The effects in two groups were evaluated after 12 weeks of treatment.Results:The levels of whole blood viscosity(high shear,middle shear and low shear),hematokrit,plasma viscosity and fibrinogen in observation group were significantly lower than those in control group(P<0.01).The levels of superoxide dismutase and malondialdehyde in observation group were significantly higher and lower than those in control group,respectively(P<0.01).The levels of endothelin,and the level of NO,non-endothelium-dependent vasodilation function induced by nitroglycerin and endothelium-dependent vasodilation function induced by blood flow in observation group were significantly lower and higher than those in control group,respectively(P<0.01).After treatment,the angina frequency,angina duration and nitroglycerin dosage in observation group were significantly lower than those in control group(P<0.01).Conclusions:Yindan Xinnaotong soft capsule in the treatment of T2DM complicated with CHD can effectively improve the hemorheology,alleviate the oxidative stress state,protect the vascular endothelial function and relieve the clinical symptoms,which is worthy of promotion in clinic.
2017, 42(11): 1476-1478.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.013
Abstract:
Objective:To observe the clinical effects of intercostal nerve block guided by ultrasound in the treatment of postherpetic neuralgia(PHN).Methods:Sixty patients with PHN were randomly divided into the group A and group B(30 cases each group).Group B was treated with intercostal nerve block guided by ultrasound combined with 10 mL of 1% ropivacaine,500 μg of vitamin B12 and 25 mg of triamcinolone,and group A was treated with simple intercrostal nerve block using the same drug,once a week,3 weeks a course.The scores of risual analogue scale(VAS) and life quality before and after treatment,and effective rate of treatment between two groups were compared.Results:After treatment,the scores of VAS and life quality in two groups significantly decreased and increased compared with before treatment,respectively(P<0.01).After treatment,the decreasing degree of VAS score in group B was significantly higher than that in group A(P<0.01),and the increasing degree of life quality(including diet,sleep,mood,daily life and life interest) score in group B was significantly higher than that in group A(P<0.01).The pain remittence in group B was significantly better than that in group A after treatment(P<0.01).Conclusions:The intercostal nerve block guided by ultrasound in treating PHN is safe and high accuracy rate,which is worthy of promotion in clinic.
Objective:To observe the clinical effects of intercostal nerve block guided by ultrasound in the treatment of postherpetic neuralgia(PHN).Methods:Sixty patients with PHN were randomly divided into the group A and group B(30 cases each group).Group B was treated with intercostal nerve block guided by ultrasound combined with 10 mL of 1% ropivacaine,500 μg of vitamin B12 and 25 mg of triamcinolone,and group A was treated with simple intercrostal nerve block using the same drug,once a week,3 weeks a course.The scores of risual analogue scale(VAS) and life quality before and after treatment,and effective rate of treatment between two groups were compared.Results:After treatment,the scores of VAS and life quality in two groups significantly decreased and increased compared with before treatment,respectively(P<0.01).After treatment,the decreasing degree of VAS score in group B was significantly higher than that in group A(P<0.01),and the increasing degree of life quality(including diet,sleep,mood,daily life and life interest) score in group B was significantly higher than that in group A(P<0.01).The pain remittence in group B was significantly better than that in group A after treatment(P<0.01).Conclusions:The intercostal nerve block guided by ultrasound in treating PHN is safe and high accuracy rate,which is worthy of promotion in clinic.
2017, 42(11): 1479-1482.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.014
Abstract:
Objective:To investigate the relationships between the serum levels of troponin I(cTnI),lactic acid(Lac),creatine kinase(CK),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),C reactive protein(CRP),neuron-specific enolase(NSE) and mean apparent diffusion coefficient(ADC),and carbon monoxide poisoning delayed encephalopathy(DEACMP).Methods:The clinical data of 56 patients with acute carbon monoxide poisoning(ACMP) were retrospectively analyzed.The age,sex,and degree of coma of patients were collected,and the levels of COHb,Lac,cTnI,CK,CK-MB,LDH,NSE,WBC count and CRP within 24 hours of onset,and the mean ADC value of bilateral globus pallidus,around ventricle and center of semi-soft circle in MRI imaging were observed within 48 hours of admission.According to the DEACMP after 60 days,the patients were divided into onset or not of DEACMP group and non-DEACMP group.The levels of COHb,Lac,cTnI,CK,CK-MB,LDH,NSE,WBC count and CRP,and the mean ADC value of bilateral globus pallidus,around ventricle and center of semi-soft circle between two groups were compared and analyzed using logistic regression analysis method.Results:The incidence rate of coma in DEACMP group(15 cases,75.00%) was significantly higher than that in non-DEACMP group(12 cases,33.33%)(P<0.01).The levels of Lac,CK,CK-MB,LDH,CRP and NSE in DEACMP group were higher than those in non-DEACMP group(P<0.05 to P<0.01),while there was no significant difference n the levels of COHb,cTnI and WBC count between two groups(P>0.05).The ADC value of globus pallidus region in DEACMP group was significantly higher than that in non-DEACMP group(P<0.01),while there was no significant difference in the ADC values of the white matter around the lateral ventricle and central region of semi-soft circle between two groups(P>0.05).The results of logistic regression analysis showed that the coma degree,poisoning time,and levels of NSE,CK,CRP and LDH might be the independent risk factors of DEACMP,and the difference of which was statistically significant(P<0.05 to P<0.01).Conclusions:The serum levels of Lac,CK,CK-MB,LDH,CRP and NSE maybe related to the DEACMP,which has important significance in the diagnosis and prognosis of DEACMP.
Objective:To investigate the relationships between the serum levels of troponin I(cTnI),lactic acid(Lac),creatine kinase(CK),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),C reactive protein(CRP),neuron-specific enolase(NSE) and mean apparent diffusion coefficient(ADC),and carbon monoxide poisoning delayed encephalopathy(DEACMP).Methods:The clinical data of 56 patients with acute carbon monoxide poisoning(ACMP) were retrospectively analyzed.The age,sex,and degree of coma of patients were collected,and the levels of COHb,Lac,cTnI,CK,CK-MB,LDH,NSE,WBC count and CRP within 24 hours of onset,and the mean ADC value of bilateral globus pallidus,around ventricle and center of semi-soft circle in MRI imaging were observed within 48 hours of admission.According to the DEACMP after 60 days,the patients were divided into onset or not of DEACMP group and non-DEACMP group.The levels of COHb,Lac,cTnI,CK,CK-MB,LDH,NSE,WBC count and CRP,and the mean ADC value of bilateral globus pallidus,around ventricle and center of semi-soft circle between two groups were compared and analyzed using logistic regression analysis method.Results:The incidence rate of coma in DEACMP group(15 cases,75.00%) was significantly higher than that in non-DEACMP group(12 cases,33.33%)(P<0.01).The levels of Lac,CK,CK-MB,LDH,CRP and NSE in DEACMP group were higher than those in non-DEACMP group(P<0.05 to P<0.01),while there was no significant difference n the levels of COHb,cTnI and WBC count between two groups(P>0.05).The ADC value of globus pallidus region in DEACMP group was significantly higher than that in non-DEACMP group(P<0.01),while there was no significant difference in the ADC values of the white matter around the lateral ventricle and central region of semi-soft circle between two groups(P>0.05).The results of logistic regression analysis showed that the coma degree,poisoning time,and levels of NSE,CK,CRP and LDH might be the independent risk factors of DEACMP,and the difference of which was statistically significant(P<0.05 to P<0.01).Conclusions:The serum levels of Lac,CK,CK-MB,LDH,CRP and NSE maybe related to the DEACMP,which has important significance in the diagnosis and prognosis of DEACMP.
2017, 42(11): 1483-1485,1490.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.015
Abstract:
Objective:To systematically evaluate the clinical efficacy of intermittent pneumatic compression(IPC) in preventing deep vein thrombosis(DVT) of postoperative patients.Methods:Databases including Cochrane library,PubMed,Embase,International Network of Agencies for Health Technology Assessment(INAHTA),Chinese National Knowledge Infrastructure(CNKI),Wanfang Data and VIP were used to collect papers which accord with the inclusive criteria published on January 1st,1990 to May 10th,2015.Papers related to randomized control trial were undertaken quality assessment by Cochrane Handbook 5.3,and those systematic evaluation and health technology assessment ones were extracted results directly.Results:The effect of IPC on preventing DVT of postoperative patients is obvious,but there was no accordant conclusion compared with the preventing effects of elastic stocking and drugs.The effect of IPC combined with drugs on preventing DVT was better than that of single use of drugs or IPC.Conclusions:The effect of useing IPC only and using IPC combined with drugs on preventing DVT is both obvious.IPC can be used as an alternative therapy of drugs to prevent DVT to some extent.
Objective:To systematically evaluate the clinical efficacy of intermittent pneumatic compression(IPC) in preventing deep vein thrombosis(DVT) of postoperative patients.Methods:Databases including Cochrane library,PubMed,Embase,International Network of Agencies for Health Technology Assessment(INAHTA),Chinese National Knowledge Infrastructure(CNKI),Wanfang Data and VIP were used to collect papers which accord with the inclusive criteria published on January 1st,1990 to May 10th,2015.Papers related to randomized control trial were undertaken quality assessment by Cochrane Handbook 5.3,and those systematic evaluation and health technology assessment ones were extracted results directly.Results:The effect of IPC on preventing DVT of postoperative patients is obvious,but there was no accordant conclusion compared with the preventing effects of elastic stocking and drugs.The effect of IPC combined with drugs on preventing DVT was better than that of single use of drugs or IPC.Conclusions:The effect of useing IPC only and using IPC combined with drugs on preventing DVT is both obvious.IPC can be used as an alternative therapy of drugs to prevent DVT to some extent.
2017, 42(11): 1486-1490.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.016
Abstract:
Objective:To investigate the effects of different concentrations of dexmedetomidine(DEX) on early cognitive function and inflammatory parameters in elderly patients treated with orthopedic surgery.Methods:Ninety elderly patients treated with general anesthesia operation,aged 60 to 85 years,ASA Ⅰ~Ⅲ and without severe liver and kidney disease and active inflammation,were divided into the 0.5 μg/kg DEX group(groupD1),1.0 μg/kg DEX group(group D2) and control group(group C) (30 cases each group).The preoperative and postoperative cognitive function in three groups were evaluated using Mini Mental State Examination(MMSE).The inflammatory parameters[including serum interleukin -1β(IL-1β),serum interleukin -6(IL-6),tumor necrosis factor -α(TNF-α) and high-sensitivity C-reactive protein(hs-CRP)] and oxidative stress parameters[including serum super-oxide anion(O2-),hydroxy radical(OH),malondialdehyde(MDA) and super-oxide dismutase(SOD)] between three groups before 5 min of anesthesia(T0),after 30 min of begining operation(T1),at the end of operation(T2),postoperative 2 h(T3),postoperative 1 d(T4) and postoperative 3 d(T5) were compared.Results:The differences of inflammatory parameters between three groups before operation were not statistically significant(P>0.05).Compared with before operation,all parameters in group C gradually increased after 3 d of operation.The serum levels of IL-1β in group D1 at T2 and T3 increased,but which was lower than that group C.The serum levels of IL-6,TNF-αand hs-CRP in group D1 gradually increased from T1 to T5,but which was lower than that in group C.There was not significant change of the levels of IL-1β in group D2 at each time-point,and the levels of IL-6,TNF-α and hs-CRP in group D2 at T2,T3,from T1 to T3 and from T1 to T5 gradually increased with compared before operation.The differences of the oxidative stress parameters between three groups with before operation were not statistically significant.Compared with before operation,the levels of O2- and MDA in group D1 and D2 gradually increased from T2 to T5,and which in group D2 was lower than that in group D1 and C.Compared with before operation,the level of SOD gradually decreased,and the level of SOD in group D2 was higher than that in group D1 and C.Compared with before operation,the level of OH in group D1 and D2 gradually increased,which in group D2 and D1 from T1 to T5 were lower that in group C.Conclusions:DEX can reduce the early cognitive dyfunction in elderly patients treated with orthopedic surgery by inhibiting oxidative stress and inflammation reaction.
Objective:To investigate the effects of different concentrations of dexmedetomidine(DEX) on early cognitive function and inflammatory parameters in elderly patients treated with orthopedic surgery.Methods:Ninety elderly patients treated with general anesthesia operation,aged 60 to 85 years,ASA Ⅰ~Ⅲ and without severe liver and kidney disease and active inflammation,were divided into the 0.5 μg/kg DEX group(groupD1),1.0 μg/kg DEX group(group D2) and control group(group C) (30 cases each group).The preoperative and postoperative cognitive function in three groups were evaluated using Mini Mental State Examination(MMSE).The inflammatory parameters[including serum interleukin -1β(IL-1β),serum interleukin -6(IL-6),tumor necrosis factor -α(TNF-α) and high-sensitivity C-reactive protein(hs-CRP)] and oxidative stress parameters[including serum super-oxide anion(O2-),hydroxy radical(OH),malondialdehyde(MDA) and super-oxide dismutase(SOD)] between three groups before 5 min of anesthesia(T0),after 30 min of begining operation(T1),at the end of operation(T2),postoperative 2 h(T3),postoperative 1 d(T4) and postoperative 3 d(T5) were compared.Results:The differences of inflammatory parameters between three groups before operation were not statistically significant(P>0.05).Compared with before operation,all parameters in group C gradually increased after 3 d of operation.The serum levels of IL-1β in group D1 at T2 and T3 increased,but which was lower than that group C.The serum levels of IL-6,TNF-αand hs-CRP in group D1 gradually increased from T1 to T5,but which was lower than that in group C.There was not significant change of the levels of IL-1β in group D2 at each time-point,and the levels of IL-6,TNF-α and hs-CRP in group D2 at T2,T3,from T1 to T3 and from T1 to T5 gradually increased with compared before operation.The differences of the oxidative stress parameters between three groups with before operation were not statistically significant.Compared with before operation,the levels of O2- and MDA in group D1 and D2 gradually increased from T2 to T5,and which in group D2 was lower than that in group D1 and C.Compared with before operation,the level of SOD gradually decreased,and the level of SOD in group D2 was higher than that in group D1 and C.Compared with before operation,the level of OH in group D1 and D2 gradually increased,which in group D2 and D1 from T1 to T5 were lower that in group C.Conclusions:DEX can reduce the early cognitive dyfunction in elderly patients treated with orthopedic surgery by inhibiting oxidative stress and inflammation reaction.
2017, 42(11): 1491-1494.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.017
Abstract:
Objective:To investigate the effects of gabapentin combined with hemoperfusion in the treatment of restless leg syndrome(RLS) in hemodialysis patients.Methods:Fifty hemodialysis patients with RLS were randomly divided into the observation group and control group.All patients were treated with routine hemodialysis combinewd with symptomatic treatment.The observation group were additionally treated with 300 mg gabapentin by oral per night combined with blood perfusion a time every week for 8 weeks.The RLS,sleep and pain improvement in two groups were assessed using the index of RLS(IRLS),Pittsburgh Sleep Quality Index(PSQI) and Visual Analogue Scale/Score(VAS),respectively.The levels of ferritin,parathyroid hormone(PTH),Ca and P in two groups were measured before and after treatment.Results:After 8 weeks of treatment,the order of severity of IRLS,and scores of PSQI and VAS in observation group decreased significantly(P<0.01),the order of severity of IRLS,and score of PSQI in control group decreased significantly(P<0.01),but the difference of VAS score in control group was not statistically significant compared with before treatment(P>0.05).The decreasing degree of three indictors in observation group was more than that in control group(P<0.01).The levels of ferritin in two groups increased after treatment(P<0.01 and P<0.05),and the changes of the levels of PTH,Ca and P in two groups were not obvious(P>0.05).No severe adverse reaction in observation group was found.Conclusions:Gabapentin combined with hemoperfusion can significantly improve the RLS symptoms and sleep quality in hemodialysis patients,which has no significant adverse reaction.
Objective:To investigate the effects of gabapentin combined with hemoperfusion in the treatment of restless leg syndrome(RLS) in hemodialysis patients.Methods:Fifty hemodialysis patients with RLS were randomly divided into the observation group and control group.All patients were treated with routine hemodialysis combinewd with symptomatic treatment.The observation group were additionally treated with 300 mg gabapentin by oral per night combined with blood perfusion a time every week for 8 weeks.The RLS,sleep and pain improvement in two groups were assessed using the index of RLS(IRLS),Pittsburgh Sleep Quality Index(PSQI) and Visual Analogue Scale/Score(VAS),respectively.The levels of ferritin,parathyroid hormone(PTH),Ca and P in two groups were measured before and after treatment.Results:After 8 weeks of treatment,the order of severity of IRLS,and scores of PSQI and VAS in observation group decreased significantly(P<0.01),the order of severity of IRLS,and score of PSQI in control group decreased significantly(P<0.01),but the difference of VAS score in control group was not statistically significant compared with before treatment(P>0.05).The decreasing degree of three indictors in observation group was more than that in control group(P<0.01).The levels of ferritin in two groups increased after treatment(P<0.01 and P<0.05),and the changes of the levels of PTH,Ca and P in two groups were not obvious(P>0.05).No severe adverse reaction in observation group was found.Conclusions:Gabapentin combined with hemoperfusion can significantly improve the RLS symptoms and sleep quality in hemodialysis patients,which has no significant adverse reaction.
2017, 42(11): 1494-1496.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.018
Abstract:
Objective:To observe the clinical efficacy of floating needle combined with traditional Chinese medicine in the treatment of temporomandibular joint disorder.Methods:A total of 40 patients with temporomandibular disorders were divided into the observation group and control group(20 cases each group).The control group was treated with the floating needle,and the observation group was treated with floating needle combined with traditional Chinese medicine.The clinical efficacy and karnofsky(KPS) score before and after treatment between two groups were compared.Results:The effective rate in observation group(90.0%) was significantly higher than that in control group(60.0%)(P<0.01).Before treatment,the difference of the KPS score between two groups was not statistically significant(P>0.05).After treatment,the KPS scores in two groups significantly increased,and which in observation group was significantly higher than that in control group(P<0.01).Conclusions:The treatment of temporomandibular joint disorder with floating needle combined with traditional Chinese medicine can effectively improve the clinical efficacy and quality of life of patients,which has good clinical application value.
Objective:To observe the clinical efficacy of floating needle combined with traditional Chinese medicine in the treatment of temporomandibular joint disorder.Methods:A total of 40 patients with temporomandibular disorders were divided into the observation group and control group(20 cases each group).The control group was treated with the floating needle,and the observation group was treated with floating needle combined with traditional Chinese medicine.The clinical efficacy and karnofsky(KPS) score before and after treatment between two groups were compared.Results:The effective rate in observation group(90.0%) was significantly higher than that in control group(60.0%)(P<0.01).Before treatment,the difference of the KPS score between two groups was not statistically significant(P>0.05).After treatment,the KPS scores in two groups significantly increased,and which in observation group was significantly higher than that in control group(P<0.01).Conclusions:The treatment of temporomandibular joint disorder with floating needle combined with traditional Chinese medicine can effectively improve the clinical efficacy and quality of life of patients,which has good clinical application value.
Clinical effect observation of different approaches in the treatment of thoracolumbar burst fracture
2017, 42(11): 1497-1499.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.019
Abstract:
Objective:To investigate the clinical effects between posterior paraspinal muscle approach and conventional posterior middle approach in the treatment of thoracolumbar burst fractures.Methods:The clinical data of 60 thoracolumbar burst fractures patients followed by more than 6 months were retrospectively analyzed.Thirty cases were treated with posterior paraspinal muscle approach(observation group),and 30 cases were treated with conventional posterior middle approach(control group).The operative time,peroperative bleeding,and postoperative drainage,VAS score,anterior height of vertebral body and Cobb angle between two groups were compared,which was used to evaluate the treatment effects of two approaches.Results:The operative time,peroperative bleeding and postoperative drainage in observation group were significantly less than those in control group(P<0.01).The VAS score in observation group was significantly lower than that in control group 1 week and 6 months after operation(P<0.01).The differences of the anterior height of vertebral body and Cobb angle 1 week and 6 months after operation between two groups were not statistically significant(P>0.05).Conclusions:The treatment effect of thoracolumbar burst fracture with posterior paraspinal muscle approach and conventional posterior middle approach are ideal.The treatment of posterior paraspinal muscle approach is less bleeding,injury and pain,which is worthy of promotion in clinic.
Objective:To investigate the clinical effects between posterior paraspinal muscle approach and conventional posterior middle approach in the treatment of thoracolumbar burst fractures.Methods:The clinical data of 60 thoracolumbar burst fractures patients followed by more than 6 months were retrospectively analyzed.Thirty cases were treated with posterior paraspinal muscle approach(observation group),and 30 cases were treated with conventional posterior middle approach(control group).The operative time,peroperative bleeding,and postoperative drainage,VAS score,anterior height of vertebral body and Cobb angle between two groups were compared,which was used to evaluate the treatment effects of two approaches.Results:The operative time,peroperative bleeding and postoperative drainage in observation group were significantly less than those in control group(P<0.01).The VAS score in observation group was significantly lower than that in control group 1 week and 6 months after operation(P<0.01).The differences of the anterior height of vertebral body and Cobb angle 1 week and 6 months after operation between two groups were not statistically significant(P>0.05).Conclusions:The treatment effect of thoracolumbar burst fracture with posterior paraspinal muscle approach and conventional posterior middle approach are ideal.The treatment of posterior paraspinal muscle approach is less bleeding,injury and pain,which is worthy of promotion in clinic.
2017, 42(11): 1500-1502.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.020
Abstract:
Objective:To analyze the epidemiological and clinical features of tinea manus and pedis.Methods:Two hundred and fourteen patients with tinea manus and pedis were set as research object to retrospective analysis,to find out the epidemilology and clinical characteristics of tinea manus and pedis.Results:More than 90% of the patients were infected by Trichophyton rubrum(tinea manus 63.1%,tinea pedis 71.0%) and Trichophyton tonsurans(tinea manus 9.8%,tinea pedis 15.4%).All patients with purely tinea manus were not checkouted with epidermophyton floccosum.Compared to purely tinea pedis,relevance ratio of candida albicans in purely tinea manus is obviously higher(tinea manus 22.0%,tinea pedis 5.6%).According to the degree of skin damage,tinea manus and pedis are divided into blister type,scale type and erosion type.As of epidemiology,20-40 years old are the high risk group,which contain 69.6% and about 45% are workman.In morbidity,scale type(tinea manus 62.1%,tinea pedis 77.1%) > erosion type(tinea manus 22.9%,tinea pedis 13.1%) > blister type(tinea manus 15.0%,tinea pedis 9.8%).Conclusions:Most of the tinea manus and pedis is caused by a minority of pathogenic bacterium.The morbidity have close relationship to what they touch and personal hygiene.Both the prevalence of tinea pedis and tinea manus have a rule of composition that is scale type >erosion type > blisters type.
Objective:To analyze the epidemiological and clinical features of tinea manus and pedis.Methods:Two hundred and fourteen patients with tinea manus and pedis were set as research object to retrospective analysis,to find out the epidemilology and clinical characteristics of tinea manus and pedis.Results:More than 90% of the patients were infected by Trichophyton rubrum(tinea manus 63.1%,tinea pedis 71.0%) and Trichophyton tonsurans(tinea manus 9.8%,tinea pedis 15.4%).All patients with purely tinea manus were not checkouted with epidermophyton floccosum.Compared to purely tinea pedis,relevance ratio of candida albicans in purely tinea manus is obviously higher(tinea manus 22.0%,tinea pedis 5.6%).According to the degree of skin damage,tinea manus and pedis are divided into blister type,scale type and erosion type.As of epidemiology,20-40 years old are the high risk group,which contain 69.6% and about 45% are workman.In morbidity,scale type(tinea manus 62.1%,tinea pedis 77.1%) > erosion type(tinea manus 22.9%,tinea pedis 13.1%) > blister type(tinea manus 15.0%,tinea pedis 9.8%).Conclusions:Most of the tinea manus and pedis is caused by a minority of pathogenic bacterium.The morbidity have close relationship to what they touch and personal hygiene.Both the prevalence of tinea pedis and tinea manus have a rule of composition that is scale type >erosion type > blisters type.
2017, 42(11): 1503-1506.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.021
Abstract:
Objective:To analyze the risk factors and intervention measures of gestational diabetes mellitus,and reduce the incidence of gestational diabetes mellitus.Methods:Three hundred and twenty-six pregnant women were divided into the pregnancy complicated with diabetes mellitus group(case group,54 cases) and non-pregnant diabetes mellitus group(control group,272 cases).The clinical data of pregnant women were collected,the influencing factors of the incidence of gestational diabetes were analyzed using the single factor and multi-factor logistic regression analysis methods.Results:The high fasting blood glucose in early pregnancy,high fasting insulin level,high insulin resistance index,age ≥ 35,high body mass before pregnancy,high body mass index before pregnancy,family history of diabetes mellitus,high body weight increasing in early pregnancy and high body weight increasing in second trimester were the independent risk factors of gestational diabetes mellitus(P<0.05 to P<0.01).Conclusions:Developing diet management and controlling body weight during the health care before pregnancy should be implemented.Controlling body weight,blood lipid and fasting blood glucose should be managed in early pregnancy.
Objective:To analyze the risk factors and intervention measures of gestational diabetes mellitus,and reduce the incidence of gestational diabetes mellitus.Methods:Three hundred and twenty-six pregnant women were divided into the pregnancy complicated with diabetes mellitus group(case group,54 cases) and non-pregnant diabetes mellitus group(control group,272 cases).The clinical data of pregnant women were collected,the influencing factors of the incidence of gestational diabetes were analyzed using the single factor and multi-factor logistic regression analysis methods.Results:The high fasting blood glucose in early pregnancy,high fasting insulin level,high insulin resistance index,age ≥ 35,high body mass before pregnancy,high body mass index before pregnancy,family history of diabetes mellitus,high body weight increasing in early pregnancy and high body weight increasing in second trimester were the independent risk factors of gestational diabetes mellitus(P<0.05 to P<0.01).Conclusions:Developing diet management and controlling body weight during the health care before pregnancy should be implemented.Controlling body weight,blood lipid and fasting blood glucose should be managed in early pregnancy.
2017, 42(11): 1506-1509.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.022
Abstract:
Objective:To investigate the risk factors and preventive measures of postmenopausal osteoporosis(PMOP).Methods:The bone density of lumbar spine and hip in 176 patients diagnosed by PMOP were measured.The age,height,weight,duration of menopause,fertility and movement in all cases were analyzed.The influencing factors were investigated using backward iteration method.The ordinal logistic model was established using the reduction of bone mineral density as the dependent variable.The examining factors were substituted into the model,the relationship between the related factor and PMOP was analyzed,and the relevant preventive measures were explored.Results:The age,weight,duration of menopause,fertility,eating habits,present exercise habits,young exercise habits had significant explanatory power on the dependent variable.The age,duration of menopause and fertility were positively correlated to the bone reduce,and risk factors of disease.The body weight,diet,present movement and youth movement were negatively correlated with the bone reduction,and protective factors of disease.Conclusions:Improving the diet structure,increasing the weight and fat content and effective planning the daily movement of PMOP patients can effectively reduce the falling speed of bone mineral density,and control the incidence of PMOP.
Objective:To investigate the risk factors and preventive measures of postmenopausal osteoporosis(PMOP).Methods:The bone density of lumbar spine and hip in 176 patients diagnosed by PMOP were measured.The age,height,weight,duration of menopause,fertility and movement in all cases were analyzed.The influencing factors were investigated using backward iteration method.The ordinal logistic model was established using the reduction of bone mineral density as the dependent variable.The examining factors were substituted into the model,the relationship between the related factor and PMOP was analyzed,and the relevant preventive measures were explored.Results:The age,weight,duration of menopause,fertility,eating habits,present exercise habits,young exercise habits had significant explanatory power on the dependent variable.The age,duration of menopause and fertility were positively correlated to the bone reduce,and risk factors of disease.The body weight,diet,present movement and youth movement were negatively correlated with the bone reduction,and protective factors of disease.Conclusions:Improving the diet structure,increasing the weight and fat content and effective planning the daily movement of PMOP patients can effectively reduce the falling speed of bone mineral density,and control the incidence of PMOP.
2017, 42(11): 1510-1512,1516.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.023
Abstract:
Objective:To investigate the risk factors of the occurrence of arteriovenous fistula embolization in maintenance hemodialysis patients.Methods:The clinical data of 137 patients treated with the first arteriovenous fistula forming operation combined with maintenance hemodialysis were retrospectively analyzed.Twenty-four cases with arteriovenous fistula embolization and 113 cases without arteriovenous fistula embolization were divided into the observation group and control group,respectively.The clinical data and life index between two groups were compared.Results:The differences of cardiovascular and cerebrovascular diseases,diabetes mellitus,frequency of dialysis,L-carnitine usage,platelet count,albumin,triglyceride,low density lipoprotein,C reactive protein,parathyroid hormone,serum phosphorus,and calcium-phosphorus product between two groups were statistically significantusage(P<0.05 to P<0.01).The results of the logistic regression analysis showed that the diabetes mellitus,L-carnitine usage,low density lipoprotein,calcium-phosphorus product and serum phosphorus entered into the logistic regression model were the risk factors of affecting the occurrence of arteriovenous fistula embolization(P<0.05 to P<0.01).Conclusions:The diabetes mellitus,low density lipoprotein increasing,high calcium-phosphorus product and serum phosphorus increasing are the independent risk factors of the occurrence of arteriovenous fistula embolization in maintenance hemodialysis patients,and the use of L-carnitine is the protective factor of the occurrence of arteriovenous fistula embolization in maintenance hemodialysis patients.
Objective:To investigate the risk factors of the occurrence of arteriovenous fistula embolization in maintenance hemodialysis patients.Methods:The clinical data of 137 patients treated with the first arteriovenous fistula forming operation combined with maintenance hemodialysis were retrospectively analyzed.Twenty-four cases with arteriovenous fistula embolization and 113 cases without arteriovenous fistula embolization were divided into the observation group and control group,respectively.The clinical data and life index between two groups were compared.Results:The differences of cardiovascular and cerebrovascular diseases,diabetes mellitus,frequency of dialysis,L-carnitine usage,platelet count,albumin,triglyceride,low density lipoprotein,C reactive protein,parathyroid hormone,serum phosphorus,and calcium-phosphorus product between two groups were statistically significantusage(P<0.05 to P<0.01).The results of the logistic regression analysis showed that the diabetes mellitus,L-carnitine usage,low density lipoprotein,calcium-phosphorus product and serum phosphorus entered into the logistic regression model were the risk factors of affecting the occurrence of arteriovenous fistula embolization(P<0.05 to P<0.01).Conclusions:The diabetes mellitus,low density lipoprotein increasing,high calcium-phosphorus product and serum phosphorus increasing are the independent risk factors of the occurrence of arteriovenous fistula embolization in maintenance hemodialysis patients,and the use of L-carnitine is the protective factor of the occurrence of arteriovenous fistula embolization in maintenance hemodialysis patients.
2017, 42(11): 1513-1516.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.024
Abstract:
Objective:To investigate the role and significance of Th17/Treg and its related cytokine levels in the pathogenesis of wheezing in infants.Methods:Sixty acute attack wheezing infants,50 lower respiratory infection infants without wheezing and 50 healthy infants were divided into the wheezing group,nonwheezing group and control group,respectively.According to the number of wheezing,the wheezing group was subdivided into the group A1(first wheezing group,34 cases) and group A2(repeat wheezing group,wheezing number ≥ 2 times,26 cases).According to the risk factor of wheezing,the wheezing group was subdivided into the group B1(complicated with high risk factor group,25 cases) and group B2(complicated without high risk factor group,35 cases).The proportions of Th17 and Treg cells in peripheral blood in all groups were detected using flow cytometry,and the serum levels of interleukin-17(IL-17) and interleukin-10(IL-10) in all group were detected using ELISA.Results:The differences of the percentages of Th17 and Treg cells,and Treg/Th17 between three groups were statistically significant(P<0.01).The Treg cells and percentage of Treg/Th17 in wheezing group and nonwheezing group were significantly lower than those in control group(P<0.01),and the percentage of Th17 cells in wheezing group and nonwheezing group were significantly higher than that in control group(P<0.01).The Treg cells and percentage of Treg/Th17 in wheezing group were significantly lower than those in nonwheezing group(P<0.01),and the percentage of Th17 cells in wheezing group was significantly higher than that in nonwheezing group(P<0.01).The differences of the serum levels of IL-17 and IL-10 between three groups were statistically significant(P<0.01).The serum levels of IL-17 in wheezing group and nonwheezing group were significantly higher than that in control group(P<0.01),and the serum levels of IL-10 in wheezing group and nonwheezing group were significantly lower than that in control group(P<0.01).The serum level of IL-17 in wheezing group was significantly higher than that in nonwheezing group(P<0.01),and the serum level of IL-10 in wheezing group was significantly lower than that in nonwheezing group(P<0.01).The percentage of Th17 cells in group A1 was higher than that in group A2(P<0.01),the Treg cells and percentage of Treg/Th17 in group A1 were significantly lower than those in group A2(P<0.01).The IL-17 level in group A1 was significantly higher than that in group A2(P<0.01),and the IL-10 level in group A1 was significantly lower than that in group A2(P<0.01).The percentage of Th17 cells in group B1 was significantly higher than that in group B2(P<0.01),and the Treg cells and percentage of Treg/Th17 in group B1 were significantly lower than those in group B2(P<0.01).The IL-17 level in group B1 was significantly higher than that in group B2(P<0.01),and the IL-10 level in group B1 was significantly lower than that in group B2(P<0.01).Conclusions:Early detection of Th17/Treg and related cytokines in peripheral blood may provide the basis of early diagnosis and intervention for children with bronchial asthma development tendency.
Objective:To investigate the role and significance of Th17/Treg and its related cytokine levels in the pathogenesis of wheezing in infants.Methods:Sixty acute attack wheezing infants,50 lower respiratory infection infants without wheezing and 50 healthy infants were divided into the wheezing group,nonwheezing group and control group,respectively.According to the number of wheezing,the wheezing group was subdivided into the group A1(first wheezing group,34 cases) and group A2(repeat wheezing group,wheezing number ≥ 2 times,26 cases).According to the risk factor of wheezing,the wheezing group was subdivided into the group B1(complicated with high risk factor group,25 cases) and group B2(complicated without high risk factor group,35 cases).The proportions of Th17 and Treg cells in peripheral blood in all groups were detected using flow cytometry,and the serum levels of interleukin-17(IL-17) and interleukin-10(IL-10) in all group were detected using ELISA.Results:The differences of the percentages of Th17 and Treg cells,and Treg/Th17 between three groups were statistically significant(P<0.01).The Treg cells and percentage of Treg/Th17 in wheezing group and nonwheezing group were significantly lower than those in control group(P<0.01),and the percentage of Th17 cells in wheezing group and nonwheezing group were significantly higher than that in control group(P<0.01).The Treg cells and percentage of Treg/Th17 in wheezing group were significantly lower than those in nonwheezing group(P<0.01),and the percentage of Th17 cells in wheezing group was significantly higher than that in nonwheezing group(P<0.01).The differences of the serum levels of IL-17 and IL-10 between three groups were statistically significant(P<0.01).The serum levels of IL-17 in wheezing group and nonwheezing group were significantly higher than that in control group(P<0.01),and the serum levels of IL-10 in wheezing group and nonwheezing group were significantly lower than that in control group(P<0.01).The serum level of IL-17 in wheezing group was significantly higher than that in nonwheezing group(P<0.01),and the serum level of IL-10 in wheezing group was significantly lower than that in nonwheezing group(P<0.01).The percentage of Th17 cells in group A1 was higher than that in group A2(P<0.01),the Treg cells and percentage of Treg/Th17 in group A1 were significantly lower than those in group A2(P<0.01).The IL-17 level in group A1 was significantly higher than that in group A2(P<0.01),and the IL-10 level in group A1 was significantly lower than that in group A2(P<0.01).The percentage of Th17 cells in group B1 was significantly higher than that in group B2(P<0.01),and the Treg cells and percentage of Treg/Th17 in group B1 were significantly lower than those in group B2(P<0.01).The IL-17 level in group B1 was significantly higher than that in group B2(P<0.01),and the IL-10 level in group B1 was significantly lower than that in group B2(P<0.01).Conclusions:Early detection of Th17/Treg and related cytokines in peripheral blood may provide the basis of early diagnosis and intervention for children with bronchial asthma development tendency.
2017, 42(11): 1517-1521.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.025
Abstract:
Objective:To investigate the effects of the modified Yangmo decoction combined with estrogen on the endometrial receptivity and uterine hemodynamics in patients with kidney deficiency and blood stasis type thin endometrium.Methods:Seventy patients with kidney deficiency and blood stasis type thin endometrium were randomly divided into the combined group and control group(35 cases each group).The control group were treated with estrogen supplement,and the combined group were additionally treated with the modified Yangmo decoction based on the control group.The levels of sex hormone and endometrial vascular endothelial growth factor,uterine hemodynamics,endometrial thickness and shape,pregnancy outcome,and adverse reactions between two groups were compared.Results:After 3 months of treatment,the differences of the levels of estradiol and endometrial vascular endothelial growth factor,uterine spiral artery resistance index and endometrial thickness between two groups were statistically significant(P<0.01).The ratios of type A and type B endometrium,and ratio of type C in combined group increased and decreased after treatment,respectively(P<0.01).Conclusions:The modified Yangmo decoction combined with estrogen can improve the endometrial blood supply,promote endometrial growth and improve endometrial receptivity.
Objective:To investigate the effects of the modified Yangmo decoction combined with estrogen on the endometrial receptivity and uterine hemodynamics in patients with kidney deficiency and blood stasis type thin endometrium.Methods:Seventy patients with kidney deficiency and blood stasis type thin endometrium were randomly divided into the combined group and control group(35 cases each group).The control group were treated with estrogen supplement,and the combined group were additionally treated with the modified Yangmo decoction based on the control group.The levels of sex hormone and endometrial vascular endothelial growth factor,uterine hemodynamics,endometrial thickness and shape,pregnancy outcome,and adverse reactions between two groups were compared.Results:After 3 months of treatment,the differences of the levels of estradiol and endometrial vascular endothelial growth factor,uterine spiral artery resistance index and endometrial thickness between two groups were statistically significant(P<0.01).The ratios of type A and type B endometrium,and ratio of type C in combined group increased and decreased after treatment,respectively(P<0.01).Conclusions:The modified Yangmo decoction combined with estrogen can improve the endometrial blood supply,promote endometrial growth and improve endometrial receptivity.
2017, 42(11): 1524-1526.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.027
Abstract:
Objective:To investigate the clinical features of the cerebral palsy complicated with hearing impairment and its early intervention measures in children.Methods:The hearing and auditory brainstem response(ABR) in children diagnosed by cerebral palsy were examined from May 2011 to January 2014.The hearing impairment children were treated with early intervention,and the ABR of children were recorded after two months of rehabilitation.Results:Thirty-four children with deaf in 100 children were diagnosed.The mild hearing impairment in 2 cases(5.9%) were found,which included 1 case of sensorineural deafness,1 case of retrocochlear deafness and 2 cases of both ears.The moderate hearing impairment in 5 cases(14.7%) were found,which included 4 case of sensorineural deafness,1 case of retrocochlear deafness,4 cases of both ears and 1 case of one ear.The severe hearing impairment in 3 cases(8.8%) were found,which was sensorineural deafness and in both ears.The extreme severe hearing impairment in 24 cases(70.6%) were found,which included 16 case of sensorineural deafness,8 case of retrocochlear deafness,21 cases of both ears and 3 cases of one ear.The proportion of the severe and extreme severe hearing impairment children in 34 cases with cerebral palsy complicated with hearing impairment significnatly decreased after 2 months of rehabilitation.The difference of the degree of hearing impairment in children between before and after intrvention was statistically significant(P<0.01).Conclusions:Among the children with cerebral palsy complicated with hearing impairment,the sensorineural and retrocochlear deafness is the majority,and the proportion of the extreme severe hearing impairment children is high.The early rehabilitation intervention training can significantly improve the hearing impairment in children with cerebral palsy,which is worthy of use in clinic.
Objective:To investigate the clinical features of the cerebral palsy complicated with hearing impairment and its early intervention measures in children.Methods:The hearing and auditory brainstem response(ABR) in children diagnosed by cerebral palsy were examined from May 2011 to January 2014.The hearing impairment children were treated with early intervention,and the ABR of children were recorded after two months of rehabilitation.Results:Thirty-four children with deaf in 100 children were diagnosed.The mild hearing impairment in 2 cases(5.9%) were found,which included 1 case of sensorineural deafness,1 case of retrocochlear deafness and 2 cases of both ears.The moderate hearing impairment in 5 cases(14.7%) were found,which included 4 case of sensorineural deafness,1 case of retrocochlear deafness,4 cases of both ears and 1 case of one ear.The severe hearing impairment in 3 cases(8.8%) were found,which was sensorineural deafness and in both ears.The extreme severe hearing impairment in 24 cases(70.6%) were found,which included 16 case of sensorineural deafness,8 case of retrocochlear deafness,21 cases of both ears and 3 cases of one ear.The proportion of the severe and extreme severe hearing impairment children in 34 cases with cerebral palsy complicated with hearing impairment significnatly decreased after 2 months of rehabilitation.The difference of the degree of hearing impairment in children between before and after intrvention was statistically significant(P<0.01).Conclusions:Among the children with cerebral palsy complicated with hearing impairment,the sensorineural and retrocochlear deafness is the majority,and the proportion of the extreme severe hearing impairment children is high.The early rehabilitation intervention training can significantly improve the hearing impairment in children with cerebral palsy,which is worthy of use in clinic.
2017, 42(11): 1536-1539.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.032
Abstract:
Objective:To analyze the influencing factors of nure's quality of life(QOL) from 4 hospitals of Lu'an city,and provide a scientific basis for improving QOL of nurses.Methods:Five hundred and twenty nurses from 4 hospitals of Lu'an from July 2016 to October 2016 were investigated using cluster random sampling method.The effective questionnaires were 472,and the effective rate of which was 90.8%.Results:The differences of the scores of physical,mental,social,enviromental fields of QOL and the overall feeling score in different ages nurses were not statistically significant(P>0.05).The scores of the social fields of QOL in married nurses were higher than those in unmarried nurses(P<0.05),while there was no statistical significance in other fields(P>0.05).The scores of mental,social and enviromental fields of QOL in nurses with monthly salary more than 4 000 yuan were more high(P<0.05).The scores of mental,social and enviromental fields in specialized chief nurse with different position were more high(P<0.05).The score of mental and social fields of QOL in nurses with night shift for 1 to 4 times a month was higher than that in nurses without night shift,and with night shift more than 4 times a month(P<0.05).Conclusions:The related department of hospital should pay more attention to the nurses with low salary,low working position and more than four times of night shift a month,and take effective measures to improve the QOL of nurses.
Objective:To analyze the influencing factors of nure's quality of life(QOL) from 4 hospitals of Lu'an city,and provide a scientific basis for improving QOL of nurses.Methods:Five hundred and twenty nurses from 4 hospitals of Lu'an from July 2016 to October 2016 were investigated using cluster random sampling method.The effective questionnaires were 472,and the effective rate of which was 90.8%.Results:The differences of the scores of physical,mental,social,enviromental fields of QOL and the overall feeling score in different ages nurses were not statistically significant(P>0.05).The scores of the social fields of QOL in married nurses were higher than those in unmarried nurses(P<0.05),while there was no statistical significance in other fields(P>0.05).The scores of mental,social and enviromental fields of QOL in nurses with monthly salary more than 4 000 yuan were more high(P<0.05).The scores of mental,social and enviromental fields in specialized chief nurse with different position were more high(P<0.05).The score of mental and social fields of QOL in nurses with night shift for 1 to 4 times a month was higher than that in nurses without night shift,and with night shift more than 4 times a month(P<0.05).Conclusions:The related department of hospital should pay more attention to the nurses with low salary,low working position and more than four times of night shift a month,and take effective measures to improve the QOL of nurses.
2017, 42(11): 1539-1541,1544.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.033
Abstract:
Objective:To analyze the risk factors of multi-drug resistant organisms(MDROs) infection for providing the basis in controlling infection and formulating prevention and control measures.Methods:The distribution,pathogenic species and specimens origin of MDROs were investigated by targeted monitoring,and the risk factors of MDROs infections were analyzed.Results:Two hundred and sixty-seven strains of MDROs were detected in 2014.The MDROs were mostly isolated from Intensive care unit(18.73%),and the largest amount of MDROs was ESBLs-producing E.coli(58.05%).The main specimens origin were urine(36.33%) and phlegm(31.09%).The multivariate analysis showed that the long hospital stay,hypertension,using respirator,indwelling catheter and operation were the independent risk factors of MDROs infection(P< 0.05 to P< 0.01).Conclusions:The MDROs infection is related to the hospital stay time,hypertension,using respirator,indwelling catheter and operation.The relevant control measures should be implemented according to the risk factors in order to improve the efficiency of controlling hospital infection and quality of diagnosis,and promote prognosis of patients.
Objective:To analyze the risk factors of multi-drug resistant organisms(MDROs) infection for providing the basis in controlling infection and formulating prevention and control measures.Methods:The distribution,pathogenic species and specimens origin of MDROs were investigated by targeted monitoring,and the risk factors of MDROs infections were analyzed.Results:Two hundred and sixty-seven strains of MDROs were detected in 2014.The MDROs were mostly isolated from Intensive care unit(18.73%),and the largest amount of MDROs was ESBLs-producing E.coli(58.05%).The main specimens origin were urine(36.33%) and phlegm(31.09%).The multivariate analysis showed that the long hospital stay,hypertension,using respirator,indwelling catheter and operation were the independent risk factors of MDROs infection(P< 0.05 to P< 0.01).Conclusions:The MDROs infection is related to the hospital stay time,hypertension,using respirator,indwelling catheter and operation.The relevant control measures should be implemented according to the risk factors in order to improve the efficiency of controlling hospital infection and quality of diagnosis,and promote prognosis of patients.
2017, 42(11): 1542-1544.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.034
Abstract:
Objective:To detect the hemodynamics of tortuosity of vertebral artery and congenital vertebral artery variation and value of the Color Doppler Ultrasonography in diagnosis.Methods:The ultrasonic image characteristics in 27 cases of tortuosity and line variation vertebral artery were retrospectively analyzed.The eak systolic flow velocity(PSV) and resistance index(RI) of tortuosity and line variation vertebral artery were measured with ultrasonoraphy to analyze its hemodynamics change patterns.Results:The characteristic ultrasonic image in 16 cases of tortuosity vertebral artery and 11 cases of line variation vertebral artery were found.The PSV in tortuosity vertebral artery group was significantly higher than that in normal control group and contralateral normal group(P<0.01).The PSV in line variation vertebral artery group was significantly higher than that in normal control group(P<0.01).The RI in tortuosity vertebral artery group,contralateral normal group and line variation vertebral artery group were significantly higher than that in normal control group(P<0.05).Conclusions:The ultrasonic images in tortuosity and line variation vertebral artery are characteristic,the diagnostic accurate rate of ultrasonoraphy is high,which can reflect the change of hemodynamics,predict the risk of atherosclerosis plaque forming.
Objective:To detect the hemodynamics of tortuosity of vertebral artery and congenital vertebral artery variation and value of the Color Doppler Ultrasonography in diagnosis.Methods:The ultrasonic image characteristics in 27 cases of tortuosity and line variation vertebral artery were retrospectively analyzed.The eak systolic flow velocity(PSV) and resistance index(RI) of tortuosity and line variation vertebral artery were measured with ultrasonoraphy to analyze its hemodynamics change patterns.Results:The characteristic ultrasonic image in 16 cases of tortuosity vertebral artery and 11 cases of line variation vertebral artery were found.The PSV in tortuosity vertebral artery group was significantly higher than that in normal control group and contralateral normal group(P<0.01).The PSV in line variation vertebral artery group was significantly higher than that in normal control group(P<0.01).The RI in tortuosity vertebral artery group,contralateral normal group and line variation vertebral artery group were significantly higher than that in normal control group(P<0.05).Conclusions:The ultrasonic images in tortuosity and line variation vertebral artery are characteristic,the diagnostic accurate rate of ultrasonoraphy is high,which can reflect the change of hemodynamics,predict the risk of atherosclerosis plaque forming.
2017, 42(11): 1545-1548.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.035
Abstract:
Objective:To investigate the clinical value of ultrasound measuring the extension diameter and thickness of occluder in interventional treatment of congenital heart disease(CHD).Methods:The clinical data of 90 patients treated with occluder were retrospectively analyzed.The patients were divided into the standard group(67 cases treated with occluder model followed by the consensus of experts) and non-standard group(23 cases treated with occluder model no followed by the consensus of experts) according to "common congenital heart disease interventional Chinese experts consensus".The operation time,ultrasonic examination time,hemodynamics,ultrasound measuring the extension diameter and thickness of occluder,and position of the occluder between two groups were compared.Results:There were no statistical significances in the ultrasonography time,pulmonary blood volume/systemic blood volume and pulmonary artery mean pressure between two groups(P>0.05),and the operation time in standard group was lower than that in non-standard group(P<0.05).After 2 days and 3 months of operation,the extension diameter and maximum defect diameter of occluder in standard group and after 3 months of operation in non-standard group significantly decreased(P<0.01).After 2 days and 3 months of operation,the extension diameter and maximum defect diameter of occluder in standard group were less than those in non-standard group(P<0.05 to P<0.01).The thickness of the atrial septal defect occluder type 16 to 30 mm in CHD disease was more than that in occluder type ≤ 15 mm(P<0.05).After 3 months of placing occluder,the rates of left atrium shift and total position change of occluder in non-standard group were higher than those in standard group(P<0.05 and P<0.01).Conclusions:Ultrasound can accurately and easily measure the extension diameter and thickness of occluder,effectively monitor the occluder deformation process,and assess the clinical efficacy of occluder and postoperative status.
Objective:To investigate the clinical value of ultrasound measuring the extension diameter and thickness of occluder in interventional treatment of congenital heart disease(CHD).Methods:The clinical data of 90 patients treated with occluder were retrospectively analyzed.The patients were divided into the standard group(67 cases treated with occluder model followed by the consensus of experts) and non-standard group(23 cases treated with occluder model no followed by the consensus of experts) according to "common congenital heart disease interventional Chinese experts consensus".The operation time,ultrasonic examination time,hemodynamics,ultrasound measuring the extension diameter and thickness of occluder,and position of the occluder between two groups were compared.Results:There were no statistical significances in the ultrasonography time,pulmonary blood volume/systemic blood volume and pulmonary artery mean pressure between two groups(P>0.05),and the operation time in standard group was lower than that in non-standard group(P<0.05).After 2 days and 3 months of operation,the extension diameter and maximum defect diameter of occluder in standard group and after 3 months of operation in non-standard group significantly decreased(P<0.01).After 2 days and 3 months of operation,the extension diameter and maximum defect diameter of occluder in standard group were less than those in non-standard group(P<0.05 to P<0.01).The thickness of the atrial septal defect occluder type 16 to 30 mm in CHD disease was more than that in occluder type ≤ 15 mm(P<0.05).After 3 months of placing occluder,the rates of left atrium shift and total position change of occluder in non-standard group were higher than those in standard group(P<0.05 and P<0.01).Conclusions:Ultrasound can accurately and easily measure the extension diameter and thickness of occluder,effectively monitor the occluder deformation process,and assess the clinical efficacy of occluder and postoperative status.
2017, 42(11): 1549-1551.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.036
Abstract:
Objective:To evaluate the effects of nursing intervention on the early screening cognition and behavior in first-degree relative of familial breast cancer patients.Methods:Two hundred and thirty first-degree relatives of breast cancer patients from the Breast Treatment Center of Chongqing Tumor Hospital were selected using the random sampling survey method.The first-degree relatives were treated with the nursing intervention for 1 year using the health belief mode.The data were collected before and after the intervention using self-designed questionnaire,and statistically analyzed.Results:Compared with before intervention,the cognitive score and participation rate of the early screening in first-degree relative of familial breast cancer patients obviously increased after inverention(P<0.01).Conclusions:Nursing intervention can effectively improve the awareness of early screening and early screening rate in first-degree relative of familial breast cancer patients,which is worthy of popularization and application.
Objective:To evaluate the effects of nursing intervention on the early screening cognition and behavior in first-degree relative of familial breast cancer patients.Methods:Two hundred and thirty first-degree relatives of breast cancer patients from the Breast Treatment Center of Chongqing Tumor Hospital were selected using the random sampling survey method.The first-degree relatives were treated with the nursing intervention for 1 year using the health belief mode.The data were collected before and after the intervention using self-designed questionnaire,and statistically analyzed.Results:Compared with before intervention,the cognitive score and participation rate of the early screening in first-degree relative of familial breast cancer patients obviously increased after inverention(P<0.01).Conclusions:Nursing intervention can effectively improve the awareness of early screening and early screening rate in first-degree relative of familial breast cancer patients,which is worthy of popularization and application.
2017, 42(11): 1552-1556,1559.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.037
Abstract:
Objective:To investigate the improving effects of the continuous nursing based on Omaha system on self-care ability in patients with intestinal obstruction caused by peritoneal dialysis peritonitis.Methods:Sixty-eight patients with intestinal obstruction caused by peritoneal dialysis peritonitis were randomly divided into the control group and observation group(34 cases each group).The control group and observation group were nursed with routine care and continuous nursing based on Omaha system,respectively.The disease knowledge,and scores of exercise of self-care agency scale(ESCA),health promoting lifestyle profile(HPLP) and short form 36 health survey questionnaire(SF-36) between two groups were compared.Results:At discharge,the differences of the disease knowledge,and scores of ESCA,HPLP and SF-36 between two groups were not statistically significant(P>0.05).After 3 months of discharge,the indicators of patients were significantly higher than those at discharge(P<0.05 to P<0.01),and the indicators in observation group were significantly higher than those in control group(P<0.05 to P<0.01).Conclusions:The continuous nursing based on Omaha system can significantly improve the disease knowledge and self-care ability,behavioral health and life quality of patients with intestinal obstruction caused by peritoneal dialysis peritonitis.
Objective:To investigate the improving effects of the continuous nursing based on Omaha system on self-care ability in patients with intestinal obstruction caused by peritoneal dialysis peritonitis.Methods:Sixty-eight patients with intestinal obstruction caused by peritoneal dialysis peritonitis were randomly divided into the control group and observation group(34 cases each group).The control group and observation group were nursed with routine care and continuous nursing based on Omaha system,respectively.The disease knowledge,and scores of exercise of self-care agency scale(ESCA),health promoting lifestyle profile(HPLP) and short form 36 health survey questionnaire(SF-36) between two groups were compared.Results:At discharge,the differences of the disease knowledge,and scores of ESCA,HPLP and SF-36 between two groups were not statistically significant(P>0.05).After 3 months of discharge,the indicators of patients were significantly higher than those at discharge(P<0.05 to P<0.01),and the indicators in observation group were significantly higher than those in control group(P<0.05 to P<0.01).Conclusions:The continuous nursing based on Omaha system can significantly improve the disease knowledge and self-care ability,behavioral health and life quality of patients with intestinal obstruction caused by peritoneal dialysis peritonitis.
2017, 42(11): 1557-1559.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.038
Abstract:
Objective:To explore the effects of de-escalation thinking mode in treating traumatic brain injury.Methods:Sixty craniocerebral trauma patients treated with de-escalation thinking mode from June 2015 to June 2016 and 60 craniocerebral trauma patients treated with traditional thinking mode from June 2014 to May 2015 were divided into the observation group and control group,respectively.The treatment success rate,medical dispute,family satisfaction rate,early warning time,emergency treatment time,venous channel establishment time and evaluation time in two groups were recorded.Results:The treatment success rate and satisfaction rate in observation group were significantly higher than those in control group,and the incidence rates of complication and nurse-patient dispute in observation group were lower than those in control group(P<0.05 to P<0.01).The warning time,emergency treatment time,establishment time of the venous channel and evaluation time in observation group were significantly shorter than those in control group(P<0.01).Conclusions:The de-escalation thinking mode can effectively shorten the early warning time of patients with craniocerebral trauma,whcih can provide the best treatment time for patients,improve the success rate of treatment,reduce the medical disputes and improve the satisfaction rate of the family members.
Objective:To explore the effects of de-escalation thinking mode in treating traumatic brain injury.Methods:Sixty craniocerebral trauma patients treated with de-escalation thinking mode from June 2015 to June 2016 and 60 craniocerebral trauma patients treated with traditional thinking mode from June 2014 to May 2015 were divided into the observation group and control group,respectively.The treatment success rate,medical dispute,family satisfaction rate,early warning time,emergency treatment time,venous channel establishment time and evaluation time in two groups were recorded.Results:The treatment success rate and satisfaction rate in observation group were significantly higher than those in control group,and the incidence rates of complication and nurse-patient dispute in observation group were lower than those in control group(P<0.05 to P<0.01).The warning time,emergency treatment time,establishment time of the venous channel and evaluation time in observation group were significantly shorter than those in control group(P<0.01).Conclusions:The de-escalation thinking mode can effectively shorten the early warning time of patients with craniocerebral trauma,whcih can provide the best treatment time for patients,improve the success rate of treatment,reduce the medical disputes and improve the satisfaction rate of the family members.
2017, 42(11): 1560-1563.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.039
Abstract:
Objective:To explore the effects of the time nursing model on the respiratory function and quality of life in elderly patients with lung cancer after operation.Methods:Ninety elderly patients with lung cancer after operation were randomly divided into the observation group and control group by random number table method(45 cases each group).The control group was nursed using routine nursing method,and the observation group was additionally nursed with the time nursing model based on the routine nursing.The incidence rate of respiratory complications,hospitalization and quality of life between two groups were compared.Results:The incidence rate of complications in control group was higher than that in observation group(P<0.05).The indexes of SpO2 and vital capacity in observation group were significantly better than those in control group(P<0.01).The indwelling time of intrathoracic drainage tube,getting out of bed time after operation and hospitalization time in observation group were significantly shorter than those in control group(P<0.01).The score of quality of life in observation group was significantly better than that in control group(P<0.01).Conclusions:The time nursing model can effectively reduce the occurrence of postoperative respiratory complications,improve respiratory function,speed up the rehabilitation,reduce the hospitalization time and improve the quality of life of elderly patients with lung cancer,which is worthy of application in clinic.
Objective:To explore the effects of the time nursing model on the respiratory function and quality of life in elderly patients with lung cancer after operation.Methods:Ninety elderly patients with lung cancer after operation were randomly divided into the observation group and control group by random number table method(45 cases each group).The control group was nursed using routine nursing method,and the observation group was additionally nursed with the time nursing model based on the routine nursing.The incidence rate of respiratory complications,hospitalization and quality of life between two groups were compared.Results:The incidence rate of complications in control group was higher than that in observation group(P<0.05).The indexes of SpO2 and vital capacity in observation group were significantly better than those in control group(P<0.01).The indwelling time of intrathoracic drainage tube,getting out of bed time after operation and hospitalization time in observation group were significantly shorter than those in control group(P<0.01).The score of quality of life in observation group was significantly better than that in control group(P<0.01).Conclusions:The time nursing model can effectively reduce the occurrence of postoperative respiratory complications,improve respiratory function,speed up the rehabilitation,reduce the hospitalization time and improve the quality of life of elderly patients with lung cancer,which is worthy of application in clinic.
2017, 42(11): 1563-1565.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.040
Abstract:
Objective:To explore the effects of group psychology counseling on the professional burnout and mental health of general nurses.Methods:Forty general nurses from 10 community hospitals were tutored with the psychological counseling from June 2015 to June 2016.The professional burnout and mental health of general nurses were evaluated using the occupational burnout scale and symptom self-rating scale before and after the intervention.Results:The personalization,emotional exhaustion,personal accomplishment and job burnout in general nurses after the intervention were significantly lower than those before the intervention(P<0.01).The scores of somatization,interpersonal sensitivity,compulsion,depression,hostility,anxiety,horror,paranoia and psychosis in general nurses after intervention were significantly lower than those before intervention(P<0.01).Conclusions:The group psychology counseling can effectively improve the professional burnout and mental health of general nurses,which helps nurses to well cope with nursing work.
Objective:To explore the effects of group psychology counseling on the professional burnout and mental health of general nurses.Methods:Forty general nurses from 10 community hospitals were tutored with the psychological counseling from June 2015 to June 2016.The professional burnout and mental health of general nurses were evaluated using the occupational burnout scale and symptom self-rating scale before and after the intervention.Results:The personalization,emotional exhaustion,personal accomplishment and job burnout in general nurses after the intervention were significantly lower than those before the intervention(P<0.01).The scores of somatization,interpersonal sensitivity,compulsion,depression,hostility,anxiety,horror,paranoia and psychosis in general nurses after intervention were significantly lower than those before intervention(P<0.01).Conclusions:The group psychology counseling can effectively improve the professional burnout and mental health of general nurses,which helps nurses to well cope with nursing work.
2017, 42(11): 1566-1568.
doi: 10.13898/j.cnki.issn.1000-2200.2017.11.041
Abstract:
Objective:To investigate the psychology status of patients with glaucoma during the perioperative period,and its influencing factors.Methods:Eighty-five glaucoma patients with operation,and 85 cases without operation were divided into the observation group and control group.The psychology status in observation group before operation,after 1 and 2 weeks of operation,and control group were evaluated using Anxiety and Depression Scale(HAD).The HAD score in observation group at different ages,genders and education degree were compared.Results:Compared with control group,the HAD scores in observation group before operation and after 1 week of operation increased(P<0.01),and the difference of the HAD score between the observation group after 2 weeks of operation and control group was not statistically significant(P>0.05).Among the observation group,the HAD score in patients <60 years old was higher than that in patients ≥ 60 years old before operation and after 1 week of operation(P<0.01),and the difference of the HAD score between two age groups after 2 weeks of operation was not statistically significant(P>0.05).The scores of HAD in patients with primary and lower secondary education level before operation and after 1 week of operation were higher than that in patients with secondary and above level(P<0.01),and the difference of the HAD score at different time between male and female was not statistically significant(P>0.05).Conclusions:The psychology status of patients with glaucoma during the perioperative period is relatively bad,and the influence of age and education degree of patients is great.
Objective:To investigate the psychology status of patients with glaucoma during the perioperative period,and its influencing factors.Methods:Eighty-five glaucoma patients with operation,and 85 cases without operation were divided into the observation group and control group.The psychology status in observation group before operation,after 1 and 2 weeks of operation,and control group were evaluated using Anxiety and Depression Scale(HAD).The HAD score in observation group at different ages,genders and education degree were compared.Results:Compared with control group,the HAD scores in observation group before operation and after 1 week of operation increased(P<0.01),and the difference of the HAD score between the observation group after 2 weeks of operation and control group was not statistically significant(P>0.05).Among the observation group,the HAD score in patients <60 years old was higher than that in patients ≥ 60 years old before operation and after 1 week of operation(P<0.01),and the difference of the HAD score between two age groups after 2 weeks of operation was not statistically significant(P>0.05).The scores of HAD in patients with primary and lower secondary education level before operation and after 1 week of operation were higher than that in patients with secondary and above level(P<0.01),and the difference of the HAD score at different time between male and female was not statistically significant(P>0.05).Conclusions:The psychology status of patients with glaucoma during the perioperative period is relatively bad,and the influence of age and education degree of patients is great.
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