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2018 Vol. 43, No. 8
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2018, 43(8): 981-983,988.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.001
Abstract:
Objective:To investigate the effects of maternal staphylococcal enterotoxin B(SEB) administration on proliferation of splenic lymphocytes of the newborn offspring rats in vitro.Methods:Pregnant rats on gestational day 16 were injected i.v.15 μg SEB,and the control group was injected using the same volume of PBS.The spleens of newborn offspring rats on the third day after delivery were acquired,and the splenic lymphocytes were separated.The splenic lymphocytes were co-cultured with ConA or SEB for 3 days in vitro.The subpopulation of splenic lymphocytes and its proliferation were detected using the flow cytometry and liquid scintillation analyzer,respectively.Results:After co-cultured with ConA or SEB in vitro,the percentage of CD4+T cells of splenic lymphocytes in SEB group significantly increased compared with the PBS group(P<0.05 to P<0.01).The percentages of CD4+TCR Vβ8.2+ T cells on the first day of culture,and CD8+TCR Vβ8.2+ T cells on the three day of culture in SEB group significantly increased compared with the contrd group(P<0.05 to P<0.01).Compared with PBS group,the stimulation of ConA or SEB significantly increased the proliferation ability of splenic lymphocytes in SEB group on the first day of culture,and significantly decreased on the second and third day of culture(P<0.05 to P<0.01).Conclusions:Maternal SEB administration during pregnancy can affect the proliferation of splenic lymphocytes in the newborn offspring rats in vitro.
Objective:To investigate the effects of maternal staphylococcal enterotoxin B(SEB) administration on proliferation of splenic lymphocytes of the newborn offspring rats in vitro.Methods:Pregnant rats on gestational day 16 were injected i.v.15 μg SEB,and the control group was injected using the same volume of PBS.The spleens of newborn offspring rats on the third day after delivery were acquired,and the splenic lymphocytes were separated.The splenic lymphocytes were co-cultured with ConA or SEB for 3 days in vitro.The subpopulation of splenic lymphocytes and its proliferation were detected using the flow cytometry and liquid scintillation analyzer,respectively.Results:After co-cultured with ConA or SEB in vitro,the percentage of CD4+T cells of splenic lymphocytes in SEB group significantly increased compared with the PBS group(P<0.05 to P<0.01).The percentages of CD4+TCR Vβ8.2+ T cells on the first day of culture,and CD8+TCR Vβ8.2+ T cells on the three day of culture in SEB group significantly increased compared with the contrd group(P<0.05 to P<0.01).Compared with PBS group,the stimulation of ConA or SEB significantly increased the proliferation ability of splenic lymphocytes in SEB group on the first day of culture,and significantly decreased on the second and third day of culture(P<0.05 to P<0.01).Conclusions:Maternal SEB administration during pregnancy can affect the proliferation of splenic lymphocytes in the newborn offspring rats in vitro.
2018, 43(8): 984-988.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.002
Abstract:
Objective:To investigate the effects of oxaliplatin(L-OHP) on the proliferation and apoptosis of mutant type hepatocellular carcinoma cell line HuH-7 and wild type hepatocellular carcinoma cell line HepG2,and the role of p53 signaling pathway-related protein.Methods:The inhibiting effects of L-OHP on the proliferation of HuH-7 and HepG2 cells were detected using MTT assay.After HuH-7 and HepG2 cells were treated with L-OHP,the morphology changes of two cell lines were observed by inverted microscope,the cell cycle distribution was measured by flow cytometry,and the expression of p53 signaling pathway-related protein was detected using Western blot.Results:L-OHP could inhibit the proliferation of HuH-7 and HepG2 cells,which showed the concentration and time-dependent manner(P<0.01).After treatment with L-OHP,the cells became round,off the wall and apoptosis.L-OHP could induce the HuH-7 and HepG2 cells stasising at S phase of cell cycle,and the proportion of cells increased with the increasing the concentration of L-OHP(P<0.01).L-OHP could activate the p53 signaling pathway,and regulate the protein expression levels of p21,Bax,Bcl-2 and p53 in HuH-7 and HepG2 cells(P<0.05),but the expression change of p-p53 in HuH-7 cells was not obvious.Conclusions:L-OHP can induce the apoptosis of hepatocellular carcinoma cells by activating the p53 signaling pathway,the mechanism of which may be related to regulating the expression of p53,p-p53,p21,Bax and Bcl-2 in p53 signaling pathway.
Objective:To investigate the effects of oxaliplatin(L-OHP) on the proliferation and apoptosis of mutant type hepatocellular carcinoma cell line HuH-7 and wild type hepatocellular carcinoma cell line HepG2,and the role of p53 signaling pathway-related protein.Methods:The inhibiting effects of L-OHP on the proliferation of HuH-7 and HepG2 cells were detected using MTT assay.After HuH-7 and HepG2 cells were treated with L-OHP,the morphology changes of two cell lines were observed by inverted microscope,the cell cycle distribution was measured by flow cytometry,and the expression of p53 signaling pathway-related protein was detected using Western blot.Results:L-OHP could inhibit the proliferation of HuH-7 and HepG2 cells,which showed the concentration and time-dependent manner(P<0.01).After treatment with L-OHP,the cells became round,off the wall and apoptosis.L-OHP could induce the HuH-7 and HepG2 cells stasising at S phase of cell cycle,and the proportion of cells increased with the increasing the concentration of L-OHP(P<0.01).L-OHP could activate the p53 signaling pathway,and regulate the protein expression levels of p21,Bax,Bcl-2 and p53 in HuH-7 and HepG2 cells(P<0.05),but the expression change of p-p53 in HuH-7 cells was not obvious.Conclusions:L-OHP can induce the apoptosis of hepatocellular carcinoma cells by activating the p53 signaling pathway,the mechanism of which may be related to regulating the expression of p53,p-p53,p21,Bax and Bcl-2 in p53 signaling pathway.
2018, 43(8): 989-992.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.003
Abstract:
Objective:To observe the effects of arsenic trioxide(As2O3) on the proliferation and gene expression of adenomatous polyposis coli(APC) in human bladder cancer T24 cells.Methods:The T24 cells were processed with 2,4 and 8μmol/L of As2O3,the proliferation inhibition rate was detected using MTT assay,the apoptosis was detected using the deoxyribose situ terminal transferase labeling,and the expression levels of APC mRNA and protein were detected using RT-PCR and Western blot,respectively.Results:The 2 to 8 μmol/L of As2O3 treated T24 cells for 72 h could effectively inhibit the cell proliferation(P<0.01),promote the cell apoptosis(P<0.01),and increase the expression of APC mRNA and protein(P<0.01).Conclusions:As2O3 (2~8μmol/L) can effectively inhibit the proliferation,and induce the apoptosis of T24 cells,the mechanism of which may be related to the upregulation of APC gene expression.
Objective:To observe the effects of arsenic trioxide(As2O3) on the proliferation and gene expression of adenomatous polyposis coli(APC) in human bladder cancer T24 cells.Methods:The T24 cells were processed with 2,4 and 8μmol/L of As2O3,the proliferation inhibition rate was detected using MTT assay,the apoptosis was detected using the deoxyribose situ terminal transferase labeling,and the expression levels of APC mRNA and protein were detected using RT-PCR and Western blot,respectively.Results:The 2 to 8 μmol/L of As2O3 treated T24 cells for 72 h could effectively inhibit the cell proliferation(P<0.01),promote the cell apoptosis(P<0.01),and increase the expression of APC mRNA and protein(P<0.01).Conclusions:As2O3 (2~8μmol/L) can effectively inhibit the proliferation,and induce the apoptosis of T24 cells,the mechanism of which may be related to the upregulation of APC gene expression.
2018, 43(8): 993-995,999.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.004
Abstract:
Objective:To investigate the application effects of the concept of enhanced recovery after surgery(ERAS) in non-small cell lung cancer patients treated with thoracoscopic lobectomy.Methods:Fifty non-small cell lung cancer patients scheduled by thoracoscopic lobectomy were randomly divided into the control group(25 cases) and observation group(25 cases).The control group were treated with simple thoracoscopic surgery,and the observation group was treated with thoracoscopic radical surgery combined with ERAS.The incidence rates of lung infection after operation,atelectasis and pleural effusion,degree of pain,postoperative hospital ization time,and total hospitalization expense between two groups were compared.Results:The pain scores in observation group were significantly lower than that in control group after 1 hour,6 hours and 12 hours of operation(P<0.05),and the difference of the intra-group comparison was not statistically significant(P>0.05).The incidence rate of postoperative complication in observation group(4%) was significantly lower than that in control group(20%)(P<0.05).The postoperative hospitalization time in observation group was significantly shorter than that in control group(P<0.05).Conclusions:The application of thoracoscopy combined with ERAS in treating non-small cell lung cancer can effectively promote the postoperative rehabilitation,reduce complications,shorten hospitalization time and reduce hospitalization expense,which is worthy of further clinical application.
Objective:To investigate the application effects of the concept of enhanced recovery after surgery(ERAS) in non-small cell lung cancer patients treated with thoracoscopic lobectomy.Methods:Fifty non-small cell lung cancer patients scheduled by thoracoscopic lobectomy were randomly divided into the control group(25 cases) and observation group(25 cases).The control group were treated with simple thoracoscopic surgery,and the observation group was treated with thoracoscopic radical surgery combined with ERAS.The incidence rates of lung infection after operation,atelectasis and pleural effusion,degree of pain,postoperative hospital ization time,and total hospitalization expense between two groups were compared.Results:The pain scores in observation group were significantly lower than that in control group after 1 hour,6 hours and 12 hours of operation(P<0.05),and the difference of the intra-group comparison was not statistically significant(P>0.05).The incidence rate of postoperative complication in observation group(4%) was significantly lower than that in control group(20%)(P<0.05).The postoperative hospitalization time in observation group was significantly shorter than that in control group(P<0.05).Conclusions:The application of thoracoscopy combined with ERAS in treating non-small cell lung cancer can effectively promote the postoperative rehabilitation,reduce complications,shorten hospitalization time and reduce hospitalization expense,which is worthy of further clinical application.
2018, 43(8): 996-999.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.005
Abstract:
Objective:To investigate the clinical efficacy and safety of paclitaxel combined with hyperthermic intraperitoneal infusion of cisplatin in the treatment of gastric cancer patients with ascites.Methods:Seventy-five gastric cancer patients with ascites were divided into the observation group and control group according to the different treatment methods.The observation group was treated with intravenous infusion of paclitaxel combined with hyperthermic intraperitoneal perfusion circulation cisplatin,and the control group was treated with intravenous infusion of paclitaxel combined with direct abdominal infusion of cisplatin.The clinical effects,level of serum carcinoembryonic antigen(CEA),quality of life,1-year cumulative survival rate and incidence rate of adverse reaction between two groups were compared.Results:The total effective rates of observation group and control group were 60.00% and 37.14%,respectively,and the difference of which was statistically significant(P<0.05).The decreasing degree of serum CEA level in observation group was more significant compared with control group(P<0.01).The survival quality in observation group was better than that in control group after treatment(P<0.05).The degree of adverse reactions(nausea and vomiting) in observation group was lighter than that in control group(P<0.01),and the difference of other adverse reactions between two groups was not statistically significant(P>0.05).The difference of 1-year cumulative survival rate between two groups was not statistically significant(P>0.05).Conclusions:The short-term clinical efficacy of paclitaxel combined with hyperthermic intraperitoneal infusion of cisplatin in the treatment of gastric cancer patients with ascites is good,which can improve the survival quality of patients,and prolong the survival time of part of patients.The adverse reaction can be tolerated by patients.
Objective:To investigate the clinical efficacy and safety of paclitaxel combined with hyperthermic intraperitoneal infusion of cisplatin in the treatment of gastric cancer patients with ascites.Methods:Seventy-five gastric cancer patients with ascites were divided into the observation group and control group according to the different treatment methods.The observation group was treated with intravenous infusion of paclitaxel combined with hyperthermic intraperitoneal perfusion circulation cisplatin,and the control group was treated with intravenous infusion of paclitaxel combined with direct abdominal infusion of cisplatin.The clinical effects,level of serum carcinoembryonic antigen(CEA),quality of life,1-year cumulative survival rate and incidence rate of adverse reaction between two groups were compared.Results:The total effective rates of observation group and control group were 60.00% and 37.14%,respectively,and the difference of which was statistically significant(P<0.05).The decreasing degree of serum CEA level in observation group was more significant compared with control group(P<0.01).The survival quality in observation group was better than that in control group after treatment(P<0.05).The degree of adverse reactions(nausea and vomiting) in observation group was lighter than that in control group(P<0.01),and the difference of other adverse reactions between two groups was not statistically significant(P>0.05).The difference of 1-year cumulative survival rate between two groups was not statistically significant(P>0.05).Conclusions:The short-term clinical efficacy of paclitaxel combined with hyperthermic intraperitoneal infusion of cisplatin in the treatment of gastric cancer patients with ascites is good,which can improve the survival quality of patients,and prolong the survival time of part of patients.The adverse reaction can be tolerated by patients.
2018, 43(8): 1000-1002.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.006
Abstract:
Objective:To study the relationship between the distant pain and thoracolumbar compression fractures.Methods:Sixty-five patients with thoracolumbar compression fractures were divided into observation group(13 cases) and control group(52 cases) according to the distant pain.The multifidus muscle tissue near the operation incision were detected using immunohistochemistry to identified the correlation of neurotransmitter with septal part pain.The compression degree of injury vertebra was calculated by the ratio of the height of vertebral anterior to posterior margin.The VAS score,ODI index and JOA between two groups before and after operation were compared.Results:There was no statistical significance in the compression degree of injury vertebral between two groups before and after operation(P>0.05),the compression degree of injured vertebra in two groups after operation was significantly improved compared with before operation(P<0.01).Before operation,the differences of the VAS and JOA score scores between two groups were not statistically significant(P>0.05),and the ODI index in observation group was significantly higher than that in control group(P<0.05).After operation,the score of VAS and ODI index in observation group was significantly higher than that in control group(P<0.01),and the score of JOA in observation group was significantly lower than that in control group(P<0.01).The results of immunohistochemistry showed that the antibodies of SP,CGRP and IL-6 were positive,the brown granules in cytoplasm of vascular endothelial cell and tissue adjacent to blood vessels were found in observation group.The antibodies of SP,CGRP and IL-6 in control group were negative.Conclusions:The SP,CGRP and IL-6 neurotransmitter-mediated inflammatory response may be a stimulus to the posterior ramus of spinal nerve,which may be the key cause of pain in distal part of thoracolumbar compression fractures.
Objective:To study the relationship between the distant pain and thoracolumbar compression fractures.Methods:Sixty-five patients with thoracolumbar compression fractures were divided into observation group(13 cases) and control group(52 cases) according to the distant pain.The multifidus muscle tissue near the operation incision were detected using immunohistochemistry to identified the correlation of neurotransmitter with septal part pain.The compression degree of injury vertebra was calculated by the ratio of the height of vertebral anterior to posterior margin.The VAS score,ODI index and JOA between two groups before and after operation were compared.Results:There was no statistical significance in the compression degree of injury vertebral between two groups before and after operation(P>0.05),the compression degree of injured vertebra in two groups after operation was significantly improved compared with before operation(P<0.01).Before operation,the differences of the VAS and JOA score scores between two groups were not statistically significant(P>0.05),and the ODI index in observation group was significantly higher than that in control group(P<0.05).After operation,the score of VAS and ODI index in observation group was significantly higher than that in control group(P<0.01),and the score of JOA in observation group was significantly lower than that in control group(P<0.01).The results of immunohistochemistry showed that the antibodies of SP,CGRP and IL-6 were positive,the brown granules in cytoplasm of vascular endothelial cell and tissue adjacent to blood vessels were found in observation group.The antibodies of SP,CGRP and IL-6 in control group were negative.Conclusions:The SP,CGRP and IL-6 neurotransmitter-mediated inflammatory response may be a stimulus to the posterior ramus of spinal nerve,which may be the key cause of pain in distal part of thoracolumbar compression fractures.
2018, 43(8): 1003-1006.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.007
Abstract:
Objective:To discuss the application effects of thromboelastography(TEG) in postoperative anticoagulation therapy in intracranial rupture aneurysm embolization assisted by stent.Methods:Sixty patients with rupture intracranial aneurysms treated with stent-assisted embolization were selected.All patients were treated with 300 mg of aspirin and 300 mg of clopidogrel before surgery,stent-assisted for 24 to 36 h,and divided into the control group and observation group(30 cases each group).The control group and observation group were treated with 100 mg of aspirin and 75 mg of clopidogrel by oral one time a day,TEG was used to detect the arachidonic acid(AA)-and adenosine diphosphate(ADP)-induced inhibition rate of platelet aggregation in the observation group after 24 h,48 h and 72 h after treatment,and the dose of anti-platelet aggregation drugs was adjusted according to the test results.All patients were followed up for 3 months,the change of platelet inhibition rate was observed after adjusting drug dose.The incidence rate of intracranial hemorrhage and ischemia events between two groups were compared.Results:After adjusting drug dose,the platelet inhibition rate in observation group was improved significantly(P<0.05 to P<0.01),and the difference of the low reaction inhibition rate of ADP was not statistically significant(P>0.05).The incidence rates of intracranial hemorrhage and ischemia in observation group were lower than those in control group(P<0.05).Conclusions:TEG can provide the reference in monitoring the anti-aplatelet aggregation in patients treated with rupture aneurysm assisted by stent,guide adjusting the dose of anti-platelet aggregation drug to reduce the incidence rate of intracranial hemorrhage and ischemia,and improve the prognosis.
Objective:To discuss the application effects of thromboelastography(TEG) in postoperative anticoagulation therapy in intracranial rupture aneurysm embolization assisted by stent.Methods:Sixty patients with rupture intracranial aneurysms treated with stent-assisted embolization were selected.All patients were treated with 300 mg of aspirin and 300 mg of clopidogrel before surgery,stent-assisted for 24 to 36 h,and divided into the control group and observation group(30 cases each group).The control group and observation group were treated with 100 mg of aspirin and 75 mg of clopidogrel by oral one time a day,TEG was used to detect the arachidonic acid(AA)-and adenosine diphosphate(ADP)-induced inhibition rate of platelet aggregation in the observation group after 24 h,48 h and 72 h after treatment,and the dose of anti-platelet aggregation drugs was adjusted according to the test results.All patients were followed up for 3 months,the change of platelet inhibition rate was observed after adjusting drug dose.The incidence rate of intracranial hemorrhage and ischemia events between two groups were compared.Results:After adjusting drug dose,the platelet inhibition rate in observation group was improved significantly(P<0.05 to P<0.01),and the difference of the low reaction inhibition rate of ADP was not statistically significant(P>0.05).The incidence rates of intracranial hemorrhage and ischemia in observation group were lower than those in control group(P<0.05).Conclusions:TEG can provide the reference in monitoring the anti-aplatelet aggregation in patients treated with rupture aneurysm assisted by stent,guide adjusting the dose of anti-platelet aggregation drug to reduce the incidence rate of intracranial hemorrhage and ischemia,and improve the prognosis.
2018, 43(8): 1006-1009.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.008
Abstract:
Objective:To compare the clinical effects and adverse reactions between domestic and imported vancomycin in the treatment of severe patients with G+ coccus infection.Methods:Forty severe patients with G+ coccus infection in ICU were randomly divided into the domestic vancomycin group(group A) and imported vancomycin group(group B).The clinical data,vancomycin steady-state valley concentration,body temperature,white blood cell and renal function in two groups were recorded,and the clinical effects in two groups were evaluated after 7 and 28 days of treatment.Results:The differences of the age,sex,APACHEⅡ score,highest body temperature before using vancomycin,white blood cell,renal function(including creatinine,urea nitrogen and urine volume),primary disease,location of infection,combination use of antibiotics and mechanical ventilation between two groups were not statistically significant(P>0.05).The steady-state valley concentrations of vancomycin in group A and group B were(9.3±4.4)μg/mL and(11.9±11.1)μg/mL,respectively.At the(10-20)μg/mL of vancomycin,the cases in group A and group B were 8 and 5,respectively,the standard rate was 40% and 25%,respectively,and the difference of which was not statistically significant(P>0.05).There was no statistical significance in the decreasing degree of 72 h body temperature and white blood cell between group A and group B(P>0.05).Three acute kidney injury cases in group A and 2 acute kidney injury cases in group B were found,all 5 cases were type Ⅰ acute kidney injury,and there was no statistical significance in creatinine,urea nitrogen and urine volume between two groups among before medication,72 hours of medication and after withdrawal(P>0.05).There was no statistical significance in the 7 d and 28 d effective number of cases,clinical efficiency and length of stay in ICU between two groups (P>0.05).Conclusions:Among the severe patients with G+ coccusinfection,the level of blood drug concentration of vancomycin,clinical efficiency and nephrotoxicity between domestic and imported vancomycin treatment are sure and similar.
Objective:To compare the clinical effects and adverse reactions between domestic and imported vancomycin in the treatment of severe patients with G+ coccus infection.Methods:Forty severe patients with G+ coccus infection in ICU were randomly divided into the domestic vancomycin group(group A) and imported vancomycin group(group B).The clinical data,vancomycin steady-state valley concentration,body temperature,white blood cell and renal function in two groups were recorded,and the clinical effects in two groups were evaluated after 7 and 28 days of treatment.Results:The differences of the age,sex,APACHEⅡ score,highest body temperature before using vancomycin,white blood cell,renal function(including creatinine,urea nitrogen and urine volume),primary disease,location of infection,combination use of antibiotics and mechanical ventilation between two groups were not statistically significant(P>0.05).The steady-state valley concentrations of vancomycin in group A and group B were(9.3±4.4)μg/mL and(11.9±11.1)μg/mL,respectively.At the(10-20)μg/mL of vancomycin,the cases in group A and group B were 8 and 5,respectively,the standard rate was 40% and 25%,respectively,and the difference of which was not statistically significant(P>0.05).There was no statistical significance in the decreasing degree of 72 h body temperature and white blood cell between group A and group B(P>0.05).Three acute kidney injury cases in group A and 2 acute kidney injury cases in group B were found,all 5 cases were type Ⅰ acute kidney injury,and there was no statistical significance in creatinine,urea nitrogen and urine volume between two groups among before medication,72 hours of medication and after withdrawal(P>0.05).There was no statistical significance in the 7 d and 28 d effective number of cases,clinical efficiency and length of stay in ICU between two groups (P>0.05).Conclusions:Among the severe patients with G+ coccusinfection,the level of blood drug concentration of vancomycin,clinical efficiency and nephrotoxicity between domestic and imported vancomycin treatment are sure and similar.
2018, 43(8): 1010-1012,1015.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.009
Abstract:
Objective:To analyze the relationship between the severity of coronary atherosclerotic plaque and serum calcium level.Methods:The patients with coronary atherosclerotic heart disease diagnosed by coronary arteriography were divided into the acute myocardial infarction(AMI) group,unstable angina pectoris group(UA) and stable angina group(ASP),and the normal coronary artery was set as the control group.The severity of coronary artery lesion was assessed using SYNTAX scoring system.Results:The serum calcium content in angina group was significantly lower than that in control group(P<0.05),and the serum calcium level in AMI group was significantly lower than that in UA group and ASP group(P<0.05).The serum calcium level was positive correlation with TG(P<0.05),significantly negative correlation with SYNTAX score.The serum levels of K+,Na+ and Ca2+ were positive correlation with EF value(P<0.05 to P<0.01).The low level of serum calcium is the main one factor of coronary heart disease(P<0.05).Conclusions:The lower the serum calcium content,the higher the incidence rate of coronary atherosclerotic plaques is,and the more serious the lesion is.
Objective:To analyze the relationship between the severity of coronary atherosclerotic plaque and serum calcium level.Methods:The patients with coronary atherosclerotic heart disease diagnosed by coronary arteriography were divided into the acute myocardial infarction(AMI) group,unstable angina pectoris group(UA) and stable angina group(ASP),and the normal coronary artery was set as the control group.The severity of coronary artery lesion was assessed using SYNTAX scoring system.Results:The serum calcium content in angina group was significantly lower than that in control group(P<0.05),and the serum calcium level in AMI group was significantly lower than that in UA group and ASP group(P<0.05).The serum calcium level was positive correlation with TG(P<0.05),significantly negative correlation with SYNTAX score.The serum levels of K+,Na+ and Ca2+ were positive correlation with EF value(P<0.05 to P<0.01).The low level of serum calcium is the main one factor of coronary heart disease(P<0.05).Conclusions:The lower the serum calcium content,the higher the incidence rate of coronary atherosclerotic plaques is,and the more serious the lesion is.
2018, 43(8): 1013-1015.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.010
Abstract:
Objective:To discuss the correlation between the corrected serum sodium level and illness severity in patients with diabetic ketoacidosis(DKA).Methods:The clinical data of 134 patients with DKA were retrospectively analyzed.According to the corrected serum sodium level,the patients were divided the group G1(corrected Na level>150 mmol/L),group G2(135 mmol/L ≤ corrected Na level<150 mmol/L),group G3(125 mmol/L ≤ corrected Na level <135 mmol/L) and group G4(corrected Na level < 125 mmol/L).The plasma osmotic pressure,blood glucose,incidence rate of low blood pressure shock,incidence rate of consciousness disturbance,pH value of artery blood and HCO3- levels in all groups were compared.Results:Among G2,G3 and G4 groups,with the decreasing of the corrected serum sodium level,the plasma osmotic pressure,arterial blood pH and concentration of HCO3- gradually decreased,and the level of blood glucose gradually increased(P<0.01).But the incidence rates of blood pressure shock and disturbance of consciousness did not shon statistical difference(P>0.05).There was no statistical significance in levels of blood glucose,arterial blood pH and concentration of HCO3- between group G1 and group G3(P>0.05).Two cases died in group G1,and no death case was found in other groups.With the corrected serum sodium level decreasing in group G1,the blood glucose gradually decreased,and the concentration of HCO3- gradually increased(P<0.01).Conclusions:The corrected sodium level can be used as a tool to evaluate illness severity in DKA patient with high sodium level.
Objective:To discuss the correlation between the corrected serum sodium level and illness severity in patients with diabetic ketoacidosis(DKA).Methods:The clinical data of 134 patients with DKA were retrospectively analyzed.According to the corrected serum sodium level,the patients were divided the group G1(corrected Na level>150 mmol/L),group G2(135 mmol/L ≤ corrected Na level<150 mmol/L),group G3(125 mmol/L ≤ corrected Na level <135 mmol/L) and group G4(corrected Na level < 125 mmol/L).The plasma osmotic pressure,blood glucose,incidence rate of low blood pressure shock,incidence rate of consciousness disturbance,pH value of artery blood and HCO3- levels in all groups were compared.Results:Among G2,G3 and G4 groups,with the decreasing of the corrected serum sodium level,the plasma osmotic pressure,arterial blood pH and concentration of HCO3- gradually decreased,and the level of blood glucose gradually increased(P<0.01).But the incidence rates of blood pressure shock and disturbance of consciousness did not shon statistical difference(P>0.05).There was no statistical significance in levels of blood glucose,arterial blood pH and concentration of HCO3- between group G1 and group G3(P>0.05).Two cases died in group G1,and no death case was found in other groups.With the corrected serum sodium level decreasing in group G1,the blood glucose gradually decreased,and the concentration of HCO3- gradually increased(P<0.01).Conclusions:The corrected sodium level can be used as a tool to evaluate illness severity in DKA patient with high sodium level.
2018, 43(8): 1016-1018,1021.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.011
Abstract:
Objective:To explore the effects of cathter-directed thrombolysis combined with intermittent pulsed anticoagulation in the treatment of acute deep venous thrombosis(DVT) of the lower extremity.Methods:The clinical data of 37 patients with lower extremity DVT were retrospectively analyzed.The patients were divided into the observation group and control group according to the use of urokinase.The observation group was injected with 48 mL of 0.9% saline injection containing 100 000 units of urokinase,4 to 8 mL/h,for 24 h,and treated additionally with low concentration of heparin pulsed flush thrombolytic catheter,2 to 3 times a day.The control group was injected within 30 min,and the heparin solution and dexamethasone were not used.Results:After 1 and 2 weeks of treatment,the remission degree of lower limb swelling in observation group was better than that in control group(P<0.01 and P<0.05).After 2 weeks of treatment,the remission degree of swelling and pain in two groups,and venous patency in observation group were significantly better than those after 1 week of treatment(P<0.01).After treatment,the arterial partial pressure of oxygen and international normalized ratio in two groups were significantly improved,and the arterial partial pressure of oxygen in observation group was higher than that in control group(P<0.01).The effective rate of treatment in observation group and control group were 65.00% and 35.29%,respectively,and the difference of which was not statistically significant(P>0.05).Among 37 patients,32 cases were followed up for 90 to 360 d,1 case with slight swelling in observation group and 4 cases with moderate swelling in control group were found.Doppler color ultrasound or lower extremity deep venography examination showed that the part of valve was damaged,and the thrombosis in femoral vein and popliteal vein remained or recurred.Conclusions:The cathter-directed thrombolysis combined with intermittent pulsed anticoagulation can relieve the swelling of lower extremity DVT,improve the venous patency,and reduce the survival and recurrence of thrombus.
Objective:To explore the effects of cathter-directed thrombolysis combined with intermittent pulsed anticoagulation in the treatment of acute deep venous thrombosis(DVT) of the lower extremity.Methods:The clinical data of 37 patients with lower extremity DVT were retrospectively analyzed.The patients were divided into the observation group and control group according to the use of urokinase.The observation group was injected with 48 mL of 0.9% saline injection containing 100 000 units of urokinase,4 to 8 mL/h,for 24 h,and treated additionally with low concentration of heparin pulsed flush thrombolytic catheter,2 to 3 times a day.The control group was injected within 30 min,and the heparin solution and dexamethasone were not used.Results:After 1 and 2 weeks of treatment,the remission degree of lower limb swelling in observation group was better than that in control group(P<0.01 and P<0.05).After 2 weeks of treatment,the remission degree of swelling and pain in two groups,and venous patency in observation group were significantly better than those after 1 week of treatment(P<0.01).After treatment,the arterial partial pressure of oxygen and international normalized ratio in two groups were significantly improved,and the arterial partial pressure of oxygen in observation group was higher than that in control group(P<0.01).The effective rate of treatment in observation group and control group were 65.00% and 35.29%,respectively,and the difference of which was not statistically significant(P>0.05).Among 37 patients,32 cases were followed up for 90 to 360 d,1 case with slight swelling in observation group and 4 cases with moderate swelling in control group were found.Doppler color ultrasound or lower extremity deep venography examination showed that the part of valve was damaged,and the thrombosis in femoral vein and popliteal vein remained or recurred.Conclusions:The cathter-directed thrombolysis combined with intermittent pulsed anticoagulation can relieve the swelling of lower extremity DVT,improve the venous patency,and reduce the survival and recurrence of thrombus.
2018, 43(8): 1019-1021.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.012
Abstract:
Objective:To investigate the effects of alprostadil on the renal function and inflammatory factors in patients with hepatorenal syndrome.Methods:A total of 76 patients with hepatorenal syndrome was randomly divided into the observation group and control group.The control group was treated with routine measure,and the observation group were treated with alprostadil on the basis of control group.The improvement of renal function and inflammatory factors between two groups were compared.Results:Before treatment,the difference of the related index between two groups was not statistically significant(P>0.05).After 2 weeks of treatment,the levels of serum total bilirubin,alanine aminotransferase,cystatin C and C-reactive protein in the observation group were lower than those in control group(P<0.01),and the levels of albumin and 24-hour urine volume in observation group were significantly higher than those in control group(P<0.01).Conclusions:The effect of alprostadil in treating the hepatorenal syndrome is reliable,which can effectively protect the renal function,significantly improve the inflammatory status,and is worthy of promotion in clinic.
Objective:To investigate the effects of alprostadil on the renal function and inflammatory factors in patients with hepatorenal syndrome.Methods:A total of 76 patients with hepatorenal syndrome was randomly divided into the observation group and control group.The control group was treated with routine measure,and the observation group were treated with alprostadil on the basis of control group.The improvement of renal function and inflammatory factors between two groups were compared.Results:Before treatment,the difference of the related index between two groups was not statistically significant(P>0.05).After 2 weeks of treatment,the levels of serum total bilirubin,alanine aminotransferase,cystatin C and C-reactive protein in the observation group were lower than those in control group(P<0.01),and the levels of albumin and 24-hour urine volume in observation group were significantly higher than those in control group(P<0.01).Conclusions:The effect of alprostadil in treating the hepatorenal syndrome is reliable,which can effectively protect the renal function,significantly improve the inflammatory status,and is worthy of promotion in clinic.
2018, 43(8): 1022-1025.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.013
Abstract:
Objective:To investigate the risk factors of contrast-induced nephropathy(CIN) in acute myocardial infarction(AMI) patients treated with emergence percutaneous coronary intervention(PCI).Methods:One hundred and ten AMI patients treated with emergency PCI were divided into the CIN group and non-CIN group.The drug dosage,basic disease,preoperative medication and general data between two groups were compared,and the related risk factors of CIN were analyzed.Results:The differences of the renal replacement therapy,stroke,blood transfusion,angina and death between two groups were not statistically significant(P>0.05).During hospitalization,the mechanical ventilation,acute heart failure,aortic balloon pacing assistance,and arrhythmias in CIN group were higher than those in non-CIN group(P<0.05 to P<0.01).The blood glucose,hypersensitive C-reactive protein(hs-CRP),creatinine kinase peak value,anemia,serum creatinine(Scr) and contrast dose (CMV) in CIN group were significantly higher than those in non-CIN group(P<0.05 to P<0.01).The glomerular filtration rate(eGFR),hemoglobin,left ventricular ejection fraction (LVEF) in CIN group were lower than those in non-CIN group(P<0.05 to P<0.01).Multivariate analysis results showed that the CMV,renal insufficiency,Scr,LVEF,eGRF,diabetes,anemia,hs-CRP and ratio of CMV/eGFR were the risk factors of the occurrence of CIN(P<0.05 to P<0.01).Conclusions:The CMV,renal insufficiency,Scr,LVEF,eGRF,diabetes,anemia,hs-CRP and ratio of CMV/eGFR in AMI patients treated with emergence PCI should be strictly controlled to decrease the occurrence of CIN.
Objective:To investigate the risk factors of contrast-induced nephropathy(CIN) in acute myocardial infarction(AMI) patients treated with emergence percutaneous coronary intervention(PCI).Methods:One hundred and ten AMI patients treated with emergency PCI were divided into the CIN group and non-CIN group.The drug dosage,basic disease,preoperative medication and general data between two groups were compared,and the related risk factors of CIN were analyzed.Results:The differences of the renal replacement therapy,stroke,blood transfusion,angina and death between two groups were not statistically significant(P>0.05).During hospitalization,the mechanical ventilation,acute heart failure,aortic balloon pacing assistance,and arrhythmias in CIN group were higher than those in non-CIN group(P<0.05 to P<0.01).The blood glucose,hypersensitive C-reactive protein(hs-CRP),creatinine kinase peak value,anemia,serum creatinine(Scr) and contrast dose (CMV) in CIN group were significantly higher than those in non-CIN group(P<0.05 to P<0.01).The glomerular filtration rate(eGFR),hemoglobin,left ventricular ejection fraction (LVEF) in CIN group were lower than those in non-CIN group(P<0.05 to P<0.01).Multivariate analysis results showed that the CMV,renal insufficiency,Scr,LVEF,eGRF,diabetes,anemia,hs-CRP and ratio of CMV/eGFR were the risk factors of the occurrence of CIN(P<0.05 to P<0.01).Conclusions:The CMV,renal insufficiency,Scr,LVEF,eGRF,diabetes,anemia,hs-CRP and ratio of CMV/eGFR in AMI patients treated with emergence PCI should be strictly controlled to decrease the occurrence of CIN.
2018, 43(8): 1026-1029.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.014
Abstract:
Objective:To investigate the effects of general anesthesia and epidural anesthesia on the postoperative cognitive function and the stress response in elderly patients.Methods:Seventy-two elderly patients treated with surgery were divided into the observation group and control group according to random number table method(36 cases each group).The observation group and control group were anesthetized using the epidural and general anesthesia,respectively.The cognitive function in two groups before and after operation was assessed using easy mental state examination table(MMES) and Wechsler memory scale(WMS).The levels of glucose(Glu) and high-sensitivity C-reactive protein(hs-CRP) in two groups at preoperative and postoperative 30 min and postoperative 6 h,and stress reaction of patient were analyzed.Results:The differences of the incidence rate of the cognitive disorder between two groups after 6h,24h and 72h of operation were not statistically significant(P>0.05).The scores of MMES and WMS in observation group after operation were higher than those before operation(P<0.05 to P<0.01),and the differences of which between before operation and after 3 d of operation was not statistically significant(P>0.05).The scores of MMES and WMS(except Memory pictures and Memory touch items) in control group after 1 d of operation were significantly higher than those before operation(P<0.01).The scores of MMES and Memory experience of WMS in observation group after 1 d of operation were significantly lower than those in control group(P<0.01),and the score of memory pictures of WMS in observation group after 1 d of operation was higher than that in control group(P<0.01).The scores of WMS(except Memory orientation and Memory touch items) in observation group after 3d of operation were significantly lower than those in control group(P<0.01).The levels of Glu and hs-CRP in observation group after 0.5h and 6 h of operation were significantly lower than those in control group(P<0.01).Conclusions:Compared with general anesthesia,the effect of epidural anesthesia on cognitive function and stress reaction is little,the dose of drug is little.The epidural anesthesia is especially appropriate for elderly patients,and worthy of clinical promotion.
Objective:To investigate the effects of general anesthesia and epidural anesthesia on the postoperative cognitive function and the stress response in elderly patients.Methods:Seventy-two elderly patients treated with surgery were divided into the observation group and control group according to random number table method(36 cases each group).The observation group and control group were anesthetized using the epidural and general anesthesia,respectively.The cognitive function in two groups before and after operation was assessed using easy mental state examination table(MMES) and Wechsler memory scale(WMS).The levels of glucose(Glu) and high-sensitivity C-reactive protein(hs-CRP) in two groups at preoperative and postoperative 30 min and postoperative 6 h,and stress reaction of patient were analyzed.Results:The differences of the incidence rate of the cognitive disorder between two groups after 6h,24h and 72h of operation were not statistically significant(P>0.05).The scores of MMES and WMS in observation group after operation were higher than those before operation(P<0.05 to P<0.01),and the differences of which between before operation and after 3 d of operation was not statistically significant(P>0.05).The scores of MMES and WMS(except Memory pictures and Memory touch items) in control group after 1 d of operation were significantly higher than those before operation(P<0.01).The scores of MMES and Memory experience of WMS in observation group after 1 d of operation were significantly lower than those in control group(P<0.01),and the score of memory pictures of WMS in observation group after 1 d of operation was higher than that in control group(P<0.01).The scores of WMS(except Memory orientation and Memory touch items) in observation group after 3d of operation were significantly lower than those in control group(P<0.01).The levels of Glu and hs-CRP in observation group after 0.5h and 6 h of operation were significantly lower than those in control group(P<0.01).Conclusions:Compared with general anesthesia,the effect of epidural anesthesia on cognitive function and stress reaction is little,the dose of drug is little.The epidural anesthesia is especially appropriate for elderly patients,and worthy of clinical promotion.
2018, 43(8): 1030-1032.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.015
Abstract:
Objective:To explore the experience of fast locating and ligating renal pedicle in anatomical retroperitoneal laparoscopic radical nephrectomy.Methods:Forty-three patients treated with retroperitoneal laparoscopic radical nephrectomy were divided into the control group(21 cases) and observation group(22 cases) according to the different ways of locating and ligating the renal pedicle.The control group were treated with retroperitoneal laparoscopic radical nephrectomy,and the renal pedicle were located at the protuberance of renal dorsal side.The observation group were treated with anatomical retroperitoneal laparoscopic radical nephrectomy,and the renal pedicle were looked for along the surface of the inferior vena cava(right side) or abdominal aorta(left side).The time of locating and ligating renal pedicle,intraoperative bleeding volume,operation time,incidence rate of intraoperative and postoperative complications,number of lymph node dissected,extracting drainage tube time and postoperative hospital stay were compared between two groups.Results:All operations were successful,and no case was open operated.The time of locating and ligating renal pedicle in observation group was significantly shorter than that in control group(P<0.01),and the number of dissecting lymph node in observation group were more than that in control group(P<0.05).There was not serious complications in two groups during perioperative and postioperative period.The differences in bleeding volume,operation time,extracting drainage tube time and postoperative hospitalization time between two groups were not statistically significant(P>0.05).Conclusions:Anatomical retroperitoneal laparoscopic radical nephrectomy combined with looking for renal pedicle along the surface of the inferior vena cava or abdominal aorta can quickly locate and ligate renal pedicle vessels and improve the number of dissecting lymph node,and the anatomy and operation vision are clear.It is helpful for standardization of surgery and rapid popularization of technology.
Objective:To explore the experience of fast locating and ligating renal pedicle in anatomical retroperitoneal laparoscopic radical nephrectomy.Methods:Forty-three patients treated with retroperitoneal laparoscopic radical nephrectomy were divided into the control group(21 cases) and observation group(22 cases) according to the different ways of locating and ligating the renal pedicle.The control group were treated with retroperitoneal laparoscopic radical nephrectomy,and the renal pedicle were located at the protuberance of renal dorsal side.The observation group were treated with anatomical retroperitoneal laparoscopic radical nephrectomy,and the renal pedicle were looked for along the surface of the inferior vena cava(right side) or abdominal aorta(left side).The time of locating and ligating renal pedicle,intraoperative bleeding volume,operation time,incidence rate of intraoperative and postoperative complications,number of lymph node dissected,extracting drainage tube time and postoperative hospital stay were compared between two groups.Results:All operations were successful,and no case was open operated.The time of locating and ligating renal pedicle in observation group was significantly shorter than that in control group(P<0.01),and the number of dissecting lymph node in observation group were more than that in control group(P<0.05).There was not serious complications in two groups during perioperative and postioperative period.The differences in bleeding volume,operation time,extracting drainage tube time and postoperative hospitalization time between two groups were not statistically significant(P>0.05).Conclusions:Anatomical retroperitoneal laparoscopic radical nephrectomy combined with looking for renal pedicle along the surface of the inferior vena cava or abdominal aorta can quickly locate and ligate renal pedicle vessels and improve the number of dissecting lymph node,and the anatomy and operation vision are clear.It is helpful for standardization of surgery and rapid popularization of technology.
2018, 43(8): 1033-1035,1038.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.016
Abstract:
Objective:To explore the clinical effects of the injured vertebra fixation combined with limited open window decompression in the treatment of thoracolumbar burst fractures.Methods:Sixty-five patients with thoracolumbar burst fractures were divided into the observation group(33 cases) and control group(32 cases).The observation group was treated with the injured vertebral internal fixation combined with limited open window decompression,and the control group was treated with across injured vertebral bone graft fixation combined with limited open window decompression.The vertebral stability,motor dysfunction and nerve function between two groups were compared.Results:After 1 and 6 months of treatment,the Cobb angle in observation group was lower than that in control group(P<0.01),and the height of intervertebral space was higher than that in control group(P<0.05).After 1,3 and 6 months of treatment,the Oswestry score in observation group was significantly lower than that in control group(P<0.05~P<0.01).After 1 and 6 months of treatment,the levels of serum neuron specific enolase and soluble S-100 protein in observation group were lower than those in control group(P<0.05 to P<0.01).After 6 months of treatment,the Frankel classification in observation group was better than that in control group(P=0.05).Conclusions:The injured vertebra fixation combined with limited open window decompression can improve the stability of vertebra and neurological function in patients with thoracolumbar burst fracture.
Objective:To explore the clinical effects of the injured vertebra fixation combined with limited open window decompression in the treatment of thoracolumbar burst fractures.Methods:Sixty-five patients with thoracolumbar burst fractures were divided into the observation group(33 cases) and control group(32 cases).The observation group was treated with the injured vertebral internal fixation combined with limited open window decompression,and the control group was treated with across injured vertebral bone graft fixation combined with limited open window decompression.The vertebral stability,motor dysfunction and nerve function between two groups were compared.Results:After 1 and 6 months of treatment,the Cobb angle in observation group was lower than that in control group(P<0.01),and the height of intervertebral space was higher than that in control group(P<0.05).After 1,3 and 6 months of treatment,the Oswestry score in observation group was significantly lower than that in control group(P<0.05~P<0.01).After 1 and 6 months of treatment,the levels of serum neuron specific enolase and soluble S-100 protein in observation group were lower than those in control group(P<0.05 to P<0.01).After 6 months of treatment,the Frankel classification in observation group was better than that in control group(P=0.05).Conclusions:The injured vertebra fixation combined with limited open window decompression can improve the stability of vertebra and neurological function in patients with thoracolumbar burst fracture.
2018, 43(8): 1036-1038.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.017
Abstract:
Objective:To investigate the clinical characteristics,treatment and prognosis of very low birth weight infants(VLBWI) complicated with late metabolic acidosis.Methods:Eighty-six infants with birth weight less than 1 500 g and more than 1 000 g were divided into the acidosis period and normal period according to the acid poisoning using the self-control analysis method.The drowsiness and feeding,facial and respiratory changes,body weight,heat,blood glucose,venous nutrition,treatment outcome and prognosis in different period infants were observed.Results:Among 86 VLBWI,it was mainly late metabolic acidosis occurred within 9-20 days old.The incidence rates of the lethargy,intaking milk decreasing,dark complexion,perioral cyanosis,irregular breathing,blood glucose increasing,weight unincreasing and heat ungain in acidosis period group were significantly higher than those in normal period group(P<0.01).The complete/partial parenteral nutrition affected on the occurrence of acidosis.The acidosis relieved in all cases after treatment with 1.4% of sodium bicarbonate.Conclusions:The late metabolic acidosis often occurs in VLBWI,the signs should be closely observed.The complete/partial breastfeeding can reduce the occurrence of acidosis.Sodium bicarbonate can be used in early prevention and symptomatic treatment.
Objective:To investigate the clinical characteristics,treatment and prognosis of very low birth weight infants(VLBWI) complicated with late metabolic acidosis.Methods:Eighty-six infants with birth weight less than 1 500 g and more than 1 000 g were divided into the acidosis period and normal period according to the acid poisoning using the self-control analysis method.The drowsiness and feeding,facial and respiratory changes,body weight,heat,blood glucose,venous nutrition,treatment outcome and prognosis in different period infants were observed.Results:Among 86 VLBWI,it was mainly late metabolic acidosis occurred within 9-20 days old.The incidence rates of the lethargy,intaking milk decreasing,dark complexion,perioral cyanosis,irregular breathing,blood glucose increasing,weight unincreasing and heat ungain in acidosis period group were significantly higher than those in normal period group(P<0.01).The complete/partial parenteral nutrition affected on the occurrence of acidosis.The acidosis relieved in all cases after treatment with 1.4% of sodium bicarbonate.Conclusions:The late metabolic acidosis often occurs in VLBWI,the signs should be closely observed.The complete/partial breastfeeding can reduce the occurrence of acidosis.Sodium bicarbonate can be used in early prevention and symptomatic treatment.
2018, 43(8): 1057-1059.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.025
Abstract:
Objective:To investigate the application value of chemiluminescent microparticle immunoassay(CMIA) in serological detection of syphilis.Methods:The special antibody of treponema pallidum in clinical specimens were screened using enzyme-linked immunosorbent assay(ELISA),and the 86 positive samples were detected by CMIA and treponema pallidum particle agglutination assay(TPPA).The detection result was analyzed,and the value of CMIA was compared with the tolulized red unheated serum test(TRUST).Results:Among 84 positive samples detected by ELISA,84 positive samples and 2 negative samples were identified using the TPPA detection.The coincidence rate of ELISA test was 97.67%,and the coincidence rate of CMIA and TPPA was 100%.The false negative rate of TRUST was higher,and the positive rate of TRUST increased significantly with the increasing of detection value of CMIA(P<0.01).Conclusions:There is certain extent false positive rate in the syphilis screened by ELISA,CMIA can be the confirmation experiment in the screening of syphilis specific antibody.TRUST is suitable for the observation of curative effect.
Objective:To investigate the application value of chemiluminescent microparticle immunoassay(CMIA) in serological detection of syphilis.Methods:The special antibody of treponema pallidum in clinical specimens were screened using enzyme-linked immunosorbent assay(ELISA),and the 86 positive samples were detected by CMIA and treponema pallidum particle agglutination assay(TPPA).The detection result was analyzed,and the value of CMIA was compared with the tolulized red unheated serum test(TRUST).Results:Among 84 positive samples detected by ELISA,84 positive samples and 2 negative samples were identified using the TPPA detection.The coincidence rate of ELISA test was 97.67%,and the coincidence rate of CMIA and TPPA was 100%.The false negative rate of TRUST was higher,and the positive rate of TRUST increased significantly with the increasing of detection value of CMIA(P<0.01).Conclusions:There is certain extent false positive rate in the syphilis screened by ELISA,CMIA can be the confirmation experiment in the screening of syphilis specific antibody.TRUST is suitable for the observation of curative effect.
2018, 43(8): 1060-1063.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.026
Abstract:
Objective:To investigate the drug resistance and genotype of Extended-spectrum β-lactamases(ESBLs) producing Escherichia coli,and provide the basis for clinical rational drug use.Methods:Among 544 Escherichia coli strains,161 ESBLs strains were identified.Bacterial susceptibility testing of ESBLs strains was detected using VITEK 2 Compact analyzer.The ESBLs strains were confirmed by double-disk synergy test,and the resistance genes were detected by PCR.Results:The resistance rate of ESBLs-producing strains to most antimicrobial agents were higher than those of non-ESBLs-producing strains(P<0.05 to P<0.01).Among 161 strains of EBSLs,CTX-M-9 genotype in 98 stains,TEM genotype in 84 strains,CTX-M-1 genotype in 62 strains,OXA-2 genotype in 15 strains,OXA-10 genotype in 7 strains,and SHV genotype in 5 strains were identified.The drug resistance rates of CTX-M-1 genotype and TEM + CTX-M-1 genotype strains to ceftazidime(43.5% and 59.1%) were significantly higher than those of CTX-M-9 genotype and TEM+CTX-M-9 genotype strains(15% and 11.8%)(P<0.05 to P<0.01).Conclusions:The TEM and CTX-M are the main genotype of ESBLs-producing Escherichia coli.The drug resistance of ESBLs-producing strains to most antibiotics is higher than that of non-ESBLs-producing strains.
Objective:To investigate the drug resistance and genotype of Extended-spectrum β-lactamases(ESBLs) producing Escherichia coli,and provide the basis for clinical rational drug use.Methods:Among 544 Escherichia coli strains,161 ESBLs strains were identified.Bacterial susceptibility testing of ESBLs strains was detected using VITEK 2 Compact analyzer.The ESBLs strains were confirmed by double-disk synergy test,and the resistance genes were detected by PCR.Results:The resistance rate of ESBLs-producing strains to most antimicrobial agents were higher than those of non-ESBLs-producing strains(P<0.05 to P<0.01).Among 161 strains of EBSLs,CTX-M-9 genotype in 98 stains,TEM genotype in 84 strains,CTX-M-1 genotype in 62 strains,OXA-2 genotype in 15 strains,OXA-10 genotype in 7 strains,and SHV genotype in 5 strains were identified.The drug resistance rates of CTX-M-1 genotype and TEM + CTX-M-1 genotype strains to ceftazidime(43.5% and 59.1%) were significantly higher than those of CTX-M-9 genotype and TEM+CTX-M-9 genotype strains(15% and 11.8%)(P<0.05 to P<0.01).Conclusions:The TEM and CTX-M are the main genotype of ESBLs-producing Escherichia coli.The drug resistance of ESBLs-producing strains to most antibiotics is higher than that of non-ESBLs-producing strains.
The value of the detection of pepsinogen and gastrin-17 in the diagnosis of astrointestinal diseases
2018, 43(8): 1064-1066.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.027
Abstract:
Objective:To analyze the diagnostic value of the pepsinogen and gastrin-17 in gastrointestinal diseases.Methods:Eighty patients with gastrointestinal diseases and 80 patients without gastrointestinal diseases were divided into the observation group and control group,respectively.The levels of pepsinogen Ⅰ,pepsinogen Ⅱ and gastrin -17 in two groups were detected using enzyme linked immunosorbent assay(ELISA),and the levels of pepsinogen and gastrin-17 between two groups were compared.Results:The levels of pepsinogen and gastrin-17 in observation group were significantly higher than those in control group(P<0.01),but the ratio of PGⅠ/PGⅡ in observation group was significantly lower than that in control group(P<0.01).The levels of PGⅠ and gastrin-17 in patients with gastritis were significantly higher than those in patients with duodenal ulcer or gastric ulcer,but the levels of PGⅡ and PGⅠ/PGⅡ in patients with gastritis were significantly lower than those in patients with duodenal ulcer or gastric ulcer(P<0.05).The level of PGⅠ in patients with duodenal ulcer was similar to the patients with gastric ulcer(P>0.05).The level of gastrin-17 in patients with duodenal ulcer was significantly lower than that in patients with gastritis or gastric ulcer(P<0.05).The ratio of PGⅠ/PGⅡ in patients with gastric ulcer was significantly higher than that in patients with gastritis or duodenal ulcer(P<0.05),and the ratio of PGⅠ/PGⅡ in patients with gastritis was similar to the patients with duodenal ulcer(P>0.05).Conclusions:The levels of pepsinogen and gastrin-17 have certain value in the diagnosis of gastrointestinal diseases,which can be widely used in clinic.
Objective:To analyze the diagnostic value of the pepsinogen and gastrin-17 in gastrointestinal diseases.Methods:Eighty patients with gastrointestinal diseases and 80 patients without gastrointestinal diseases were divided into the observation group and control group,respectively.The levels of pepsinogen Ⅰ,pepsinogen Ⅱ and gastrin -17 in two groups were detected using enzyme linked immunosorbent assay(ELISA),and the levels of pepsinogen and gastrin-17 between two groups were compared.Results:The levels of pepsinogen and gastrin-17 in observation group were significantly higher than those in control group(P<0.01),but the ratio of PGⅠ/PGⅡ in observation group was significantly lower than that in control group(P<0.01).The levels of PGⅠ and gastrin-17 in patients with gastritis were significantly higher than those in patients with duodenal ulcer or gastric ulcer,but the levels of PGⅡ and PGⅠ/PGⅡ in patients with gastritis were significantly lower than those in patients with duodenal ulcer or gastric ulcer(P<0.05).The level of PGⅠ in patients with duodenal ulcer was similar to the patients with gastric ulcer(P>0.05).The level of gastrin-17 in patients with duodenal ulcer was significantly lower than that in patients with gastritis or gastric ulcer(P<0.05).The ratio of PGⅠ/PGⅡ in patients with gastric ulcer was significantly higher than that in patients with gastritis or duodenal ulcer(P<0.05),and the ratio of PGⅠ/PGⅡ in patients with gastritis was similar to the patients with duodenal ulcer(P>0.05).Conclusions:The levels of pepsinogen and gastrin-17 have certain value in the diagnosis of gastrointestinal diseases,which can be widely used in clinic.
2018, 43(8): 1067-1068,1072.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.028
Abstract:
Objective:To evaluate the coronary artery in-stent restenosis using high definition computed tomography.Methods:The stents simulating varying degree of stenosis(50%,85% and 100%) were scanned using high definition CT,and the image quality of stent and lumen were scored.The degree of stenosis was measured,and compared with the actual degree.Results:The image quality of stent with varying degree of stenosis was higher,and the rate of available evaluation was 93.1%.The difference between the in-stent restenosis degree and actual stenosis degree was not statistical significant(P>0.05).Conclusions:The image quality of stent of high definition CT is high,which can accurately evaluate the coronary artery in-stent restenosis.
Objective:To evaluate the coronary artery in-stent restenosis using high definition computed tomography.Methods:The stents simulating varying degree of stenosis(50%,85% and 100%) were scanned using high definition CT,and the image quality of stent and lumen were scored.The degree of stenosis was measured,and compared with the actual degree.Results:The image quality of stent with varying degree of stenosis was higher,and the rate of available evaluation was 93.1%.The difference between the in-stent restenosis degree and actual stenosis degree was not statistical significant(P>0.05).Conclusions:The image quality of stent of high definition CT is high,which can accurately evaluate the coronary artery in-stent restenosis.
2018, 43(8): 1069-1072.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.029
Abstract:
Objective:To investigate the correlation of the dynamic contrast-enhanced MRI(DCE-MRI) quantitative parameters and apparent diffusion coefficient(ADC) values with clinicopathological features in cervical cancer patients.Methods:Fifty-two patients with cervical cancer and 25 inpatients without cervix disease were divided into the observation group and control group,respectively.The Ktrans,Kep,Ve and ADC values of DCE-MRI quantification parameters between two groups were compared.The change of scanning parameters in different lesions in two groups were observed.The correlation between quantitative parameters,ADC values,and clinicopathological features of cervical cancer patients were analyzed using the Spearman correlation analysis.Results:The ADC value in control group was higher than that in observation group,and the Ktrans,Kep and Ve values in control group were lower than those in observation group(P<0.01).The ADC value and Ktrans value in cervical squamous cell carcinoma were lower and higher than that in cervical adenocarcinoma,respectively(P<0.01).There was no statistical significance in the Ktrans,Kep,Ve and ADC values among the different lymph node metastasis,lymphovascular invasion,and tumor size(P>0.05).The differences of the Ktrans,Kep and ADC values in different differentiation degree cervical squamous cell carcinoma patients were statistically significant(P<0.05 to P<0.01).The results of Spearman correlation analysis showed that the differentiation degree and clinical stage of squamous cell carcinoma were negatively correlated with ADC values,the differentiation degree of squamous cell carcinoma was not correlated with the Ve value(P>0.05),and the clinical stage of squamous cell carcinoma were positively correlated with Ve value(P<0.01).Conclusions:The differentiation degree and clinical stage can be effectively evaluated according to the quantitative parameters of DCE-MRI and ADC values in cervical cancer patients.
Objective:To investigate the correlation of the dynamic contrast-enhanced MRI(DCE-MRI) quantitative parameters and apparent diffusion coefficient(ADC) values with clinicopathological features in cervical cancer patients.Methods:Fifty-two patients with cervical cancer and 25 inpatients without cervix disease were divided into the observation group and control group,respectively.The Ktrans,Kep,Ve and ADC values of DCE-MRI quantification parameters between two groups were compared.The change of scanning parameters in different lesions in two groups were observed.The correlation between quantitative parameters,ADC values,and clinicopathological features of cervical cancer patients were analyzed using the Spearman correlation analysis.Results:The ADC value in control group was higher than that in observation group,and the Ktrans,Kep and Ve values in control group were lower than those in observation group(P<0.01).The ADC value and Ktrans value in cervical squamous cell carcinoma were lower and higher than that in cervical adenocarcinoma,respectively(P<0.01).There was no statistical significance in the Ktrans,Kep,Ve and ADC values among the different lymph node metastasis,lymphovascular invasion,and tumor size(P>0.05).The differences of the Ktrans,Kep and ADC values in different differentiation degree cervical squamous cell carcinoma patients were statistically significant(P<0.05 to P<0.01).The results of Spearman correlation analysis showed that the differentiation degree and clinical stage of squamous cell carcinoma were negatively correlated with ADC values,the differentiation degree of squamous cell carcinoma was not correlated with the Ve value(P>0.05),and the clinical stage of squamous cell carcinoma were positively correlated with Ve value(P<0.01).Conclusions:The differentiation degree and clinical stage can be effectively evaluated according to the quantitative parameters of DCE-MRI and ADC values in cervical cancer patients.
2018, 43(8): 1073-1075.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.030
Abstract:
Objective:To evaluate the application value ofultrasound tracking of mitral annular displacement(TMAD) in evaluating the left ventricular function in patient with type 2 diabetes mellitus(T2DM).Methods:Twenty-seven T2DM patients and 27 healthy volunteers were investigated.The high quantity two dimensional gray scale imaging of three consecutive cardiac cycles was obtained using the echocardiography.The systolic peak displacement(Ds),maximum displacement of the midpoint of the annulus(Mid) and maximum longitudinal diameter ratio of left ventricular end diastolic diameter(Mid%) at 4 sites of mitral annulus were measured using TMAD software,and the means value of which was calculated.The LVEF was measured using real time three dimensional echocardiography,and the correlation of the parameters of TMDA(Ds,Mid and Mid%) with two/three-dimensional? image LVEF were analyzed.Results:The differences of the E peak/A peak,ratio of E/A,left ventricular end-diastolic volume,end-systolic volume and LVEF between two groups were not statistically significant(P>0.05).The Ds,Mid and Mid% value in T2DM group were lower than those in control group(P<0.05 to P<0.01).The means value of Ds,Mid and Mid% in two groups were positive correlation with two/three-dimensional LVEF(P<0.05).Conclusions:TMAD)technique can be used to evaluate the left ventricular function in patients with T2DM.
Objective:To evaluate the application value ofultrasound tracking of mitral annular displacement(TMAD) in evaluating the left ventricular function in patient with type 2 diabetes mellitus(T2DM).Methods:Twenty-seven T2DM patients and 27 healthy volunteers were investigated.The high quantity two dimensional gray scale imaging of three consecutive cardiac cycles was obtained using the echocardiography.The systolic peak displacement(Ds),maximum displacement of the midpoint of the annulus(Mid) and maximum longitudinal diameter ratio of left ventricular end diastolic diameter(Mid%) at 4 sites of mitral annulus were measured using TMAD software,and the means value of which was calculated.The LVEF was measured using real time three dimensional echocardiography,and the correlation of the parameters of TMDA(Ds,Mid and Mid%) with two/three-dimensional? image LVEF were analyzed.Results:The differences of the E peak/A peak,ratio of E/A,left ventricular end-diastolic volume,end-systolic volume and LVEF between two groups were not statistically significant(P>0.05).The Ds,Mid and Mid% value in T2DM group were lower than those in control group(P<0.05 to P<0.01).The means value of Ds,Mid and Mid% in two groups were positive correlation with two/three-dimensional LVEF(P<0.05).Conclusions:TMAD)technique can be used to evaluate the left ventricular function in patients with T2DM.
2018, 43(8): 1076-1079.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.031
Abstract:
Objective:To explore the clinical value of X-ray guided combined with ultrasound guided percutaneous biliary drainage(PTCD) in the interventional treatment of obstructive jaundice.Methods:Forty patients with obstructive jaundice were randomly divided into the pure X-ray guidance group(X-ray group) and X-ray combined with ultrasound guidance group(combined ultrasound group) using random number method(20 cases each group).The successful rate of one-time puncture,number of puncture,use of contrast agent,time of fluoroscopy and time of operation were recorded in two groups,the levels of total bilirubin(TBIL),direct bilirubin(DBIL),aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in two groups were detected,and the incidence rate of postoperative complications in two groups were observed and counted.Results:The success rate of one-time puncture in combined ultrasound group was significantly higher than that in X-ray group(P<0.05).The average puncture times,use of contrast agent,time of fluoroscopy and time of operation in combined ultrasound group were significantly less than those in X-ray group(P<0.01).The serum biochemical indexes(such as TBIL,DBIL,AST and ALT) in two groups after operation were significantly improved compared with those before operation(P<0.05 to P<0.01),but there was no statistical significance in the TBIL in two groups between before and after operation(P>0.05),and the differences of biochemical indexes in two groups after operation were not statistically significant(P>0.05).The incidence rate of postoperative complications in combined ultrasound group was significantly lower than that in X-ray group(P<0.05).Conclusions:Simple X-ray guided and combined ultrasound guided PTCD intervention in the treatment of obstructive jaundice have a better effect on reducing yellowing,but compared with simple X-ray guidance,the value of combined ultrsound guidance is more prominent,which is worthy of clinical application.
Objective:To explore the clinical value of X-ray guided combined with ultrasound guided percutaneous biliary drainage(PTCD) in the interventional treatment of obstructive jaundice.Methods:Forty patients with obstructive jaundice were randomly divided into the pure X-ray guidance group(X-ray group) and X-ray combined with ultrasound guidance group(combined ultrasound group) using random number method(20 cases each group).The successful rate of one-time puncture,number of puncture,use of contrast agent,time of fluoroscopy and time of operation were recorded in two groups,the levels of total bilirubin(TBIL),direct bilirubin(DBIL),aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in two groups were detected,and the incidence rate of postoperative complications in two groups were observed and counted.Results:The success rate of one-time puncture in combined ultrasound group was significantly higher than that in X-ray group(P<0.05).The average puncture times,use of contrast agent,time of fluoroscopy and time of operation in combined ultrasound group were significantly less than those in X-ray group(P<0.01).The serum biochemical indexes(such as TBIL,DBIL,AST and ALT) in two groups after operation were significantly improved compared with those before operation(P<0.05 to P<0.01),but there was no statistical significance in the TBIL in two groups between before and after operation(P>0.05),and the differences of biochemical indexes in two groups after operation were not statistically significant(P>0.05).The incidence rate of postoperative complications in combined ultrasound group was significantly lower than that in X-ray group(P<0.05).Conclusions:Simple X-ray guided and combined ultrasound guided PTCD intervention in the treatment of obstructive jaundice have a better effect on reducing yellowing,but compared with simple X-ray guidance,the value of combined ultrsound guidance is more prominent,which is worthy of clinical application.
2018, 43(8): 1082-1084.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.033
Abstract:
Objective:To investigate the basic quality of director and business backbone of township hospital in Bengbu Anhui,and analyze the question of human resource management,and put forward to specific suggestion and measure.Methods:A total of 72 directors and business backbones from 17 township hospitals in Bengbu were investigated by self-designed questionnaire.Results:The director and business backbone were junior college-based,and their titles were mainly primary and secondary.The 93.1% of the director and business backbone believed that the lack of talent was the most important problem in hindering the development of township hospitals,and 79.2% of investigators believed that the low medical personnel technical level was the main problem of human resource management in hospitals.Conclusions:Human resource of the township hospital in a county of Bengbu is relatively lack,and the education and professional level of the director and business backbone need to be improved.The government,colleges and primary hospitals should formulate and improve the relevant policies,and strengthen the training of free medical students in order to meet the talent demand of the basic level hospital.
Objective:To investigate the basic quality of director and business backbone of township hospital in Bengbu Anhui,and analyze the question of human resource management,and put forward to specific suggestion and measure.Methods:A total of 72 directors and business backbones from 17 township hospitals in Bengbu were investigated by self-designed questionnaire.Results:The director and business backbone were junior college-based,and their titles were mainly primary and secondary.The 93.1% of the director and business backbone believed that the lack of talent was the most important problem in hindering the development of township hospitals,and 79.2% of investigators believed that the low medical personnel technical level was the main problem of human resource management in hospitals.Conclusions:Human resource of the township hospital in a county of Bengbu is relatively lack,and the education and professional level of the director and business backbone need to be improved.The government,colleges and primary hospitals should formulate and improve the relevant policies,and strengthen the training of free medical students in order to meet the talent demand of the basic level hospital.
2018, 43(8): 1085-1087.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.034
Abstract:
Objective:To analyze the causes of the blood discarding from volunteer donors in Fuyang city,and explore the way to decrease the blood discarding rate.Methods:The data of the blood discarding from Fuyang Blood Information Management System(SHINOW9.0) were collected during the period of the year 2011 to 2015.The infectious and non-infectious factors of the blood discarding were statistically analyzed.Results:The blood discarding rate in the last five years was 8.62%,35 357.50 u blood discarding was caused by infectious factors(41%),and 50 799.02 u blood discarding was caused by non-infectious factors(59%).The alanine aminotransferase increasing(2.06%) and lipemia(4.79%) were two main factors of blood discarding.During the 5 years,the blood discarding caused by infectious factor arrived at the peak in 2013,then decreased.The blood discarding caused by non-infectious factor was changing.Conclusions:Establishing a scientific and standardized blood management mechanism,strengthening personnel quality training,and improving the quality control of collecting and supplying blood,and increasing the level of blood test can guarantee the blood quality,and reduce the blood discarding rate.
Objective:To analyze the causes of the blood discarding from volunteer donors in Fuyang city,and explore the way to decrease the blood discarding rate.Methods:The data of the blood discarding from Fuyang Blood Information Management System(SHINOW9.0) were collected during the period of the year 2011 to 2015.The infectious and non-infectious factors of the blood discarding were statistically analyzed.Results:The blood discarding rate in the last five years was 8.62%,35 357.50 u blood discarding was caused by infectious factors(41%),and 50 799.02 u blood discarding was caused by non-infectious factors(59%).The alanine aminotransferase increasing(2.06%) and lipemia(4.79%) were two main factors of blood discarding.During the 5 years,the blood discarding caused by infectious factor arrived at the peak in 2013,then decreased.The blood discarding caused by non-infectious factor was changing.Conclusions:Establishing a scientific and standardized blood management mechanism,strengthening personnel quality training,and improving the quality control of collecting and supplying blood,and increasing the level of blood test can guarantee the blood quality,and reduce the blood discarding rate.
2018, 43(8): 1088-1090.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.035
Abstract:
Objective:To investigate the effects of early intervention of pressure injury prevention procedure on the formation of pressure injury in stroke patients.Methods:sixty patients with stroke were divided into the observation group(n=30) and control group(n=30) according to hospitalization sequence.The control group were intervened using routine nursing,and the observation group were intervened using early pressure injury prevention procedure based on the control group.The occurrence and staging of pressure injury in two groups were recorded,and the satisfaction of nursing work in two groups were analyzed.Results:The pressure injury in observation group was lighter than that in control group(P<0.05).The satisfaction rates in observation group and control group were 96.7% and 86.7%,respectively,and the difference of which was not statistical significance(P>0.05).Conclusions:The early intervention of pressure injury prevention procedure can reduce the incidence rate of pressure injury,and promote the prognosis of patients.
Objective:To investigate the effects of early intervention of pressure injury prevention procedure on the formation of pressure injury in stroke patients.Methods:sixty patients with stroke were divided into the observation group(n=30) and control group(n=30) according to hospitalization sequence.The control group were intervened using routine nursing,and the observation group were intervened using early pressure injury prevention procedure based on the control group.The occurrence and staging of pressure injury in two groups were recorded,and the satisfaction of nursing work in two groups were analyzed.Results:The pressure injury in observation group was lighter than that in control group(P<0.05).The satisfaction rates in observation group and control group were 96.7% and 86.7%,respectively,and the difference of which was not statistical significance(P>0.05).Conclusions:The early intervention of pressure injury prevention procedure can reduce the incidence rate of pressure injury,and promote the prognosis of patients.
2018, 43(8): 1113-1117.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.045
Abstract:
Objective:To investigate the training situation of academic degree postgraduate of clinical medicine,to provide the basis for the improvement of training plan.Methods:The postgraduates from four medical universities in Anhui Province were investigated using self-compiled questionnaire,and the result of which was analyzed using descriptive statistics and logistic regression.Results:The reselection rate of academic degree students was 34.80%.The differences of the admission volunteer,reasons of selection,subsidy of tutor,experimental equipment,experimental instructor,occupation of tutor,subject level of tutor,project funds,times of guidance,reading literature,number of projects involved,relevance of thesis and tutor's topic,employment mentality and employment competitiveness among the students were statistically significant(P<0.05 to P<0.01).The possibility of reselecting an academic degree in students with research interest was less than that in students with adjust,no giving up learning opportunity and relieving employment pressure(P<0.01).The imperfection of equipment and inadequate project funds could reduce the possibility of reselecting an academic degree(P<0.05 to P<0.01).Compared with tutors direction,the senior schoolmates direction or no directions could reduce the possibility of reselecting an academic degree(P<0.05 to P<0.01).A reasonable times of tutors direction(3 to 5 times) per month was the promoting factor of reselecting an academic degree(P<0.05).Conclusions:Improving the experimental equipment,mentor system and training supervisory mechanism are the basis of training.The recognition degree of students on academic degree can affect the choice of academic degree.Improving the recognition degree of students on the academic degree and its importance are the initial impetus of subject development.
Objective:To investigate the training situation of academic degree postgraduate of clinical medicine,to provide the basis for the improvement of training plan.Methods:The postgraduates from four medical universities in Anhui Province were investigated using self-compiled questionnaire,and the result of which was analyzed using descriptive statistics and logistic regression.Results:The reselection rate of academic degree students was 34.80%.The differences of the admission volunteer,reasons of selection,subsidy of tutor,experimental equipment,experimental instructor,occupation of tutor,subject level of tutor,project funds,times of guidance,reading literature,number of projects involved,relevance of thesis and tutor's topic,employment mentality and employment competitiveness among the students were statistically significant(P<0.05 to P<0.01).The possibility of reselecting an academic degree in students with research interest was less than that in students with adjust,no giving up learning opportunity and relieving employment pressure(P<0.01).The imperfection of equipment and inadequate project funds could reduce the possibility of reselecting an academic degree(P<0.05 to P<0.01).Compared with tutors direction,the senior schoolmates direction or no directions could reduce the possibility of reselecting an academic degree(P<0.05 to P<0.01).A reasonable times of tutors direction(3 to 5 times) per month was the promoting factor of reselecting an academic degree(P<0.05).Conclusions:Improving the experimental equipment,mentor system and training supervisory mechanism are the basis of training.The recognition degree of students on academic degree can affect the choice of academic degree.Improving the recognition degree of students on the academic degree and its importance are the initial impetus of subject development.
2018, 43(8): 1118-1121.
doi: 10.13898/j.cnki.issn.1000-2200.2018.08.046
Abstract:
Objective:To investigate the learning attitude and influencing factors of medical students in Anhui Medical University toward the medical communication skills,and provide evidence for improving doctor-patient communication course.Methods:The grade 2015 medical undergraduates from the Second Clinical Medical College were investigated using the general condition scale and Chinese version of Communication Skills Attitude Scale(CSAS).Results:A total of 400 questionnaires were issued,370 valid questionnaires were recovered,and the effective recovery rate was 92.50%.The average score of PAS was (50.17±7.25),which was related to the gender,participating community activities,loving medicine profession,confidence in becoming a qualified doctor and good interpersonal communication skills(P<0.05 to P<0.01),and the loving medicine profession and good interpersonal communication was the independent influencing factors(P<0.05).The average score of NAS was(34.98±5.70),which was related to the gender,participating community activities and character type(P<0.05),and the gender was the independent influencing factors(P<0.05).Among the respondents,91.10% of students had never studied the interpersonal communication courses,and 255 students(75.67%) believed that the compulsory course of doctor-patient communication skills should be established.Conclusions:The medical students of Anhui Medical University have high enthusiasm in learning doctor-patient communication skills,and the compulsory courses should be established with advanced teaching and evaluation method.The clinical teachers should pay more attention to the male,introverted personality,unlike medicine profession,lack confidence of study,unparticipating in community activities,and learn positivity and negative emotion in poor interpersonal communication skills students to improve the doctor-patient communication skills and related teaching level.
Objective:To investigate the learning attitude and influencing factors of medical students in Anhui Medical University toward the medical communication skills,and provide evidence for improving doctor-patient communication course.Methods:The grade 2015 medical undergraduates from the Second Clinical Medical College were investigated using the general condition scale and Chinese version of Communication Skills Attitude Scale(CSAS).Results:A total of 400 questionnaires were issued,370 valid questionnaires were recovered,and the effective recovery rate was 92.50%.The average score of PAS was (50.17±7.25),which was related to the gender,participating community activities,loving medicine profession,confidence in becoming a qualified doctor and good interpersonal communication skills(P<0.05 to P<0.01),and the loving medicine profession and good interpersonal communication was the independent influencing factors(P<0.05).The average score of NAS was(34.98±5.70),which was related to the gender,participating community activities and character type(P<0.05),and the gender was the independent influencing factors(P<0.05).Among the respondents,91.10% of students had never studied the interpersonal communication courses,and 255 students(75.67%) believed that the compulsory course of doctor-patient communication skills should be established.Conclusions:The medical students of Anhui Medical University have high enthusiasm in learning doctor-patient communication skills,and the compulsory courses should be established with advanced teaching and evaluation method.The clinical teachers should pay more attention to the male,introverted personality,unlike medicine profession,lack confidence of study,unparticipating in community activities,and learn positivity and negative emotion in poor interpersonal communication skills students to improve the doctor-patient communication skills and related teaching level.
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