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2022 Vol. 47, No. 2
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2022, 47(2): 141-145.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.001
Abstract:
ObjectiveTo investigate the effects of miR-18b-5p on the invasion and migration of breast cancer cells by down-regulating the expression of neural precursor cell expressed developmentally down-regulated 9(NEDD9), and its mechanism. MethodsThe expression levels of miR-18b-5p in human normal breast cancer epithelial cells MCF-10A and two breast cancer cell lines of SKBR-3 and SKBR-3/PR were detected using qRT-PCR.The surface markers of SKBR-3/PR cell-derived exosomes were detected using Western blotting.The CCK8 and Transwell assay were used to investigate the effects of the transfection of SKBR-3/PR cells with miR-18b-5p inhibitor and extraction of exosomes on the proliferation, invasion and migration of SKBR-3 cells.The targeted binding sites of miR-18b-5p to NEDD9 were predicted using Bioinformatics prediction software Target Scan.Dual luciferase assay was used to analyze the targeted regulation relationship between miR-18b-5p and NEDD9.The effects of up-regulation of NEDD9 on the invasion and migration of SKBR-3 cells were detected using Transwell assay. ResultsThe expression of miR-18b-5p in breast cancer cell line was higher than that in normal cancer cell line(P < 0.01).The expression levels of CD63 and TSG101 specific proteins SKBR-3/PR in cell-derived exosomes were high(P < 0.01).The proliferation, invasion and migration of SKBR-3 cells were significantly inhibited by the extractive exosomes of SKBR-3/PR cells transfected with miR-18b-5p inhibitor(P < 0.01).The prediction results of bioinformatics software Target Scan showed that the NEDD9 3'UTR had potential binding sites with miR-18b-5p, and the miR-18b-5p could negatively regulate NEDD9 gene expression(P < 0.01).The overexpression of NEDD9 could inhibit the invasion of SKBR-3 cells(P < 0.01). ConclusionsThe miR-18b-5p can promote the invasion and migration of breast cancer cells by targeting NEDD9.
2022, 47(2): 146-151.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.002
Abstract:
ObjectiveTo explore the effect of exosomal-miRNAs from tumor necrosis factor-like weak inducer of apoptosis(TWEAK)-stimulated macrophages(TMs) on the metastasis of bladder cancer cells. MethodsThe characteristics of exosomes derived from TWEAK-stimulated TMs, THP-1 induced differentiation were observed.Through the miRNAs microarray analysis, the expressions of miRNAs in exosomes derived from TMs treated with or without TWEAK were detected.The internalization of bladder cancer ATCC5637 cells and miRNAs after the internalization were detected by the laser scanning confocal microscope.The effect of exosome from TWEAK-stimulated TMs on metastasis and signal-pathway in ATCC5637 cells were detected. ResultsTWEAK not only increased the levels of miR-17-3p in TMs and the secreted exosomes, but also resulted in an elevated levels of miR-17-3p in recipient ATCC 5637 cells, which eventually reduced the activity of the EGFR/AKT/ERK 1/2 pathway.Pre-transfection of antagomiR-17-3p in TMs substantially decreased the levels of miR-17-3p in macrophages, its secreted exosomes and the recipient ATCC 5637 cells with a concomitant enhancement of ATCC 5637metastasis, suggesting an involvement of exosomal miR-17-3p from TMs in modulating the metastasis of ATCC 5637cells.And miR-17-3p inhibited the metastasis of bladder cancer cells via the EGFR/AKT/ERK 1/2 pathway. ConclusionsTWEAK transfers exosomal miRNAs to bladder cancer cells via TMs to inhibit the EGFR/AKT/ERK 1/2 pathway, resulting in the inhibition of metastasis in bladder cancer cells.
2022, 47(2): 152-156.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.003
Abstract:
ObjectiveTo investigate the level and clinical significance of LINC00319 expression in human hepatocellular carcinoma(HCC) and analyze the potential underlying mechanisms. MethodsThe LINC00319 expression level in HCC was checked by real time quantitative PCR method, and the relationship among LINC00319 expression level, clinical pathological features and survival rate were analyzed.Transwell assay was performed to measure the invasion and migration of effects of LINC00319 on HCC cells.The expression of EMT-related protein was determined by Western blotting. ResultsThe expression level of LINC00319 in HCC tissues was significantly higher than that in the adjacent normal tissues(P < 0.01), and it was significantly associated with TNM stage and vascular infiltration(P < 0.01 and P < 0.05).Patients in the higher LINC00319 group had a worse 5-year survival than those in the lower LINC00319 group(P < 0.01).Down-expression of LINC00319 significantly inhibited the invasion and migration of HCC cells(P < 0.01).Down-expression of LINC00319 inhibited the EMT of HCC(P < 0.01). ConclusionsLINC00319 is up-regulated in HCC tissues.Its high expression is correlated with the malignant clinical pathological features.LINC00319 high-expression regulates EMT and subsequently promotes invasion and migration of HCC cells.
2022, 47(2): 156-159.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.004
Abstract:
ObjectiveTo investigate the isolation of skin-derived precursors(SKPs) from the skin of paraplegic patients, and induce it into nervous system cells. MethodsThe 2 cm2 skin samples were obtained from the incision edge in patients with thoracic vertebral fracture complicated with paraplegia or not, cut into 1-2 mm3 pieces, and then digested in trypsin for 4 hours.The epidermal layer was removed, and the cells were collected by centrifugation after blowing.The cell phenotype was detected by fluorescence histochemistry, and the cells were induced into the differentiation of the nervous system cells. ResultsThe SKPs could successfully be isolated from the skin of paraplegia patients and there was no statistical significance in the biological characteristics between paraplegics and non-paraplegics(P>0.05).Two kinds of cells from paraplegics and non-paraplegics could express the nestin, specific antigen marker, and be induced into neuron and Schwann cells. ConclusionsSKPs can be isolated from the skin of paraplegia patients, and induced into the nervous system cells.It is suggested that it has a good application prospect in autologous cell transplantation of spinal cord injury.
2022, 47(2): 160-164.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.005
Abstract:
ObjectiveTo investigate the effect of long-chain non-coding RNA(lncRNA) urothelial carcinoma is associated 1(UCA1) targeting miR-503 on proliferation and apoptosis of cardiomyocyte with hypoxia treatment. MethodsThe cardiomyocyte AC16 hypoxia model was established in vitro.The expression of UCA1 and miR-503 were detected by real-time quantitative PCR (RT-qPCR).The cell viability was measured by CCK-8.Apoptosis and cycle distribution were detected by flow cytometry.The UCA1 overexpression vector and miR-503 inhibitor were transfected into AC16 cells, respectively.And the effects of up-regulating UCA1 or down-regulating miR-503 on the proliferation and apoptosis of AC16 cells treated with hypoxic were detected.Dual luciferase reporter gene assay and RT-qPCR confirmed the targeting effect of UCA1 on miR-503. ResultsAfter treated with hypoxia, the survival rate of AC16 cells and the proportion of S-phase cells were significantly decreased, and the apoptosis rate and the proportion of G0-G1 phase cells were significantly increased (P < 0.05).After up-regulating UCA1 expression or down-regulating miR-503 expression, the survival rate and the proportion of S-phase in AC16 cells treated with hypoxia were significantly increased, while the apoptosis rate and the proportion of G0-G1 phase cells were significantly decreased (P < 0.05).miR-503 was the target gene of UCA1, and UCA1 negatively regulated the expression of miR-503. ConclusionslncRNA UCA1 can significantly improve the survival rate and inhibit the apoptosis in hypoxia-induced cardiomyocyte, which may be attributed to the targeted down-regulation of miR-503 expression.
2022, 47(2): 165-168, 172.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.006
Abstract:
ObjectiveTo analyze the risk factors influencing the excellent response(ER) in differentiated thyroid carcinoma(DTC) patients without distant metastasis after 131I therapy. MethodsOne hundred and sixty patients treated with 131I treatment were followed for 24 months, and the efficacy response of all cases after 3, 6, 12 and 24 months of treatment were evaluated.The clinicopathological differences of patients with different therapeutic stratification were analyzed using χ2 test, Fisher exact test and Mann Whitney U test, and the logistic regression analysis and ROC curve analysis were used to explore the factors affecting ER. ResultsCompared with the initial evaluation, the ER rate at the final evaluation significantly increased(P < 0.01).The proportion of patient with younger age, lower ps-Tg level, 131I treatment times < 3, 131I total dose < 500 mCi, low initial risk stratification and initial TNM stage Ⅰ-Ⅱ in ER group were higher those in non-ER(P < 0.05 to P < 0.01).The results of logistic multiple regression analysis showed the serum ps-Tg was a protective factor affecting end-stage ER(OR=0.942, P < 0.01).The areas under ROC curve of the relationship between ps-Tg and initial ER, and final ER were 0.884 and 0.762, respectively.The optimal critical values of predicting non-ER were 16.62 ng/mL and 46.37 ng/mL(P < 0.01). ConclusionsThe efficacy of 131I in the treatment of DTC patients without distant metastasis is significant.The serum ps-Tg is of great value in predicting the initial and final evaluation of curative effects.
2022, 47(2): 169-172.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.007
Abstract:
ObjectiveTo analyze the application effects of tranexamic acid combined with autologous blood resorption system in total hip arthroplasty. MethodsEighty-four advanced femoral head necrosis patients(88 hips) treated with total hip arthroplasty were divided into the observation group(42 cases treated with tranexamic acid combined with autologous blood resorption system) and control group(43 cases treated with autologous blood resorption system).The operation time, intraoperative bleeding, auto blood resorption, postoperative drainage, intraoperative blood transfusion and postoperative blood routine in two groups were recorded. ResultsThe differences of the operation time and autologous blood resorption system were not statistically significant between two groups(P>0.05).The intraoperative bleeding, postoperative drainage and intraoperative blood transfusion rate in observation group were significantly lower than those in control group(P < 0.01).The preoperative RBC, Hb and HCT levels in two groups were significantly higher than those after 1, 3 and 7 days of operation(P < 0.05).The differences in the levels of RBC, Hb and HCT before operation were not statistically significant between two groups(P>0.05).The levels of RBC and Hb after 1, 3 and 7 days of operation, and HCT levels after 1 and 7 days of operation in observation group were higher than those in control group(P < 0.01).The difference of the level of HCT after 3 days of operation was not statistically significant between two groups(P>0.05). ConclusionsThe tranexamic acid combined with autologous blood resorption system can significantly reduce the blood loss of total hip arthroplasty, reduce the blood transfusion rate, promote the recovery and shorten the length of stay of patients.
2022, 47(2): 173-175.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.008
Abstract:
ObjectiveTo investigate the cognitive changes of migraine patients and its relationship with anxiety and depression. MethodsThe cognitive function in 151 patients with migraine were evaluated using the Montreal cognitive assessment, and the patients were divided into the cognitive decline group and normal cognitive group according to the cognitive function results.The clinical condition and mental state in two groups were evaluated using the general situation questionnaire, visual analog scale(VAS), headache impact test 6, self-evaluation of depression scale(SDS) and self-evaluation of anxiety scale(SAS). ResultsAmong 151 migraine patients, 38 cases(25.2%) with cognitive impairment were found.The effects of intensity of headache on daily life were greater(P < 0.05).The differences of SDS score and SAS score between two groups were statistically significant(P < 0.05).The results of Spearman correlation analysis showed that the cognitive dysfunction was positively correlated with anxiety and depression(r=0.236, 0.281, P < 0.05). ConclusionsAmong the migraine patients with cognitive dysfunction, the severity of headache and its impact on daily life are greater, and the cognitive impairment of migraine is clearly related to the depression and anxiety.
2022, 47(2): 176-180.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.009
Abstract:
ObjectiveTo explore the value of serum miR-1225-5p in the diagnosis and prognosis of osteosarcoma. MethodsOne hudred and four patients with osteosarcoma, 45 patients with periostitis and 50 healthy people were divided into the osteosarcoma group, periostitis group and control group, respectively.The serum expression levels of miR-1225-5p in three groups were detected using RT-PCR.The median was set as the cutoff value, the osteosarcoma group was subdivided into the high expression group and low expression group.The value of miR-1225-5p in the diagnosis of osteosarcoma was analyzed using ROC curve, Kaplan-Meier analysis and Log-Rank test were used to analyze the survival, and the COX proportional risk regression model was used to analyze the factors affecting the prognosis of patients. ResultsThe relative expression level of serum miR-1225-5p in osteosarcoma group was lower than that in periostitis group and control group(P < 0.01).The expression of miR-1225-5p was correlated with the Enneking stage and tumor metastasis(P < 0.01 and P < 0.05), but the gender, age, tumor location, tumor diameter were not correlated with the WHO classification of osteosarcoma(P>0.05).The area under the curve of serum miR-1225-5p in the diagnosis of osteosarcoma was 0.785(95%CI: 0.625-0.934).When the relative expression of serum miR-1225-5p was 0.46, the Yoden index was the highest, and the sensitivity and specificity were 89.35% and 71.30%, respectively.The total survival time and disease-free survival time in high expression group were higher than those in low expression group(P < 0.05).The serum miR-1225-5p was an independent factor of OS and DFS in patients with osteosarcoma(P < 0.05 and P < 0.01). ConclusionsMiR-1225-5p plays an important role in the diagnosis of osteosarcoma.The low expression level of serum miR-1225-5p is related to the poor prognosis of osteosarcoma patients.
2022, 47(2): 180-183.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.010
Abstract:
ObjectiveTo analyze the association of Toll-like receptor 4 (TLR4) gene expression in peripheral blood mononuclear cells with disease activity of ankylosing spondylitis (AS) and rheumatoid arthritis (RA). MethodsForty patients with AS (AS group) and 40 patients with RA (RA group) were enrolled.Thirty healthy volunteers were selected as the control group.The expression levels of TLR4 mRNA in peripheral blood mononuclear cells were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR).The association of TLR4 with disease activity and laboratory indexes was analyzed. ResultsThe expression of TLR4 mRNA in peripheral blood mononuclear cells of AS group, RA group and control group decreased gradually(P < 0.01).In AS group and RA group, TLR4 mRNA expression levels in active stage was significantly higher than those in stable stage(P < 0.01).Pearson correlation analysis showed that TLR4 mRNA expression in peripheral blood mononuclear cells was positively correlated with Bath ankylosing spondylitis disease activity index score and serum concentrations of ESR, CRP and IL-17(P < 0.05 to P < 0.01), but showed no association with RF concentration and HLA-B27 positive rate(P>0.05);TLR4 mRNA expression in peripheral blood mononuclear cells of patients with RA was positively correlated with DSA 28 score and serum concentrations of ESR, RF, CRP and IL-17(P < 0.05 to P < 0.01). ConclusionsThe expression of TLR4 mRNA in peripheral blood mononuclear cells of AS and RA patients is significantly up-regulated, which is correlated with disease activity and inflammatory indexes.
2022, 47(2): 184-187.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.011
Abstract:
ObjectiveTo investigate the relationship between malnutrition-inflammatory status and quality of life in elderly hemodialysis patients. MethodsOne hundred and three elderly patients with hemodialysis were investigated using malnutrition inflammation score(MIS) combined with kidney disease quality of life short form(KDQOL-SFTM), and the relationship between the results of which and clinical nutritional, inflammatory indicators[C reactive protein(CRP), interleukin-6(IL-6), body mass index(BMI), albumin(ALB), hemoglobin] were analyzed using the correlation and multivariate linear analysis to explore the influencing factors of quality of life. ResultsThe results of MIS showed that the incidence rate of malnutrition was 83.5%(86/103).The results of ADQOL-SFTM showed that the score of kidney disease-targeted areas(KDTA) was(52.86±9.65), and the score of short form health survey questionnaire 36(SF-36) was(48.74±16.32).The results of correlation analysis showed that the SF-36 score was positively correlated with BMI score(P < 0.01), and negatively correlated with the scores of CRP, IL-6 and MIS(P < 0.05 to P < 0.01).The KDTA score was positively correlated with ALB score(P < 0.01), and negatively correlated with the scores of CRP and age(P < 0.01 and P < 0.05).The results of multiple linear regression analysis showed that the SF-36 score increased, and the scores of IL-6 and MIS decreased with the increasing of BMI.With the decreasing of CRP, the score of ALB increased, and the total KDTA score increased(P < 0.05 to P < 0.01). ConclusionsThe incidence rate of malnutrition in elderly hemodialysis patients is higher, and the quality of life is lower.The BMI, IL-6 and MIS are important influencing factors of SF-36, and the CRP and ALB are important influencing factors of KDTA.Correcting the state of malnutrition and inflammation is helpful to improve the quality of life of patients.
2022, 47(2): 188-191.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.012
Abstract:
ObjectiveTo explore the clinical value of serum kidney injury molecule-1(KIM-1) in the prediction of lupus nephritis(LN) in patients with systemic lupus erythematosus(SLE). MethodsA total of 78 SLE patients were selected.The median follow-up time was 5.9 years(1-9.5 years).Enzyme-linked immunosorbent assay was used to detect the levels of serum KIM-1 at baseline.ROC curve was drawn to analyze the value of KIM-1 in predicting LN, calculate AUC, 95% confidence interval(CI), sensitivity and specificity under ROC curve.Kaplan-Meier method was used to draw survival curve of SLE patients with different KIM-1 expression levels, and compare with log-Rank test; Cox regression analysis was used to determine the factors of LN. ResultsDuring the follow-up period, LN occurred in 38 patients(48.72%).The serum KIM-1 concentration in LN group was(46.02±6.28) ng/L, which was significantly higher than that in non-LN group(23.56±5.29) ng/L(P < 0.01).With 42.35 ng/L as the cutoff value, KIM-1 had a better effect on predicting LN in SLE patients(AUC: 0.715, 95%CI: 0.589-0.841;sensitivity and specificity 82.01% and 56.33%, respectively).LN occurred in 37 cases(71.15%) in high KIM-1 group.LN occurred in 8 cases(30.77%) in low KIM-1 group.The median time from SLE diagnosis to LN in high KIM-1 group was 6.7 years(4.8-8.5 years), significantly shorter than that in low KIM-1 group[median time was 8.8 years(7.7-9.7 years)](P < 0.05).KIM-1 is an independent influencing factor of LN(HR=2.301, 95%CI: 1.235-3.964, P < 0.01). ConclusionsThe level of serum KIM-1 was significantly increased in patients with SLE complicated with LN, which may be an effective biomarker for the prediction of LN.
2022, 47(2): 192-195.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.013
Abstract:
ObjectiveTo analyze the relationship between serum level of cortisol(COR) and disease severity of acute exacerbations of chronic obstructive pulmonary disease(AECOPD). MethodsTwo hundred and eight patients with COPD were divided into the stable COPD group(100 cases) and AECOPD group(108 cases) according to the condition of COPD.The AECOPD group was subdivided into the mild group(n=34), moderate group(n=35) and severe group(n=39), and 100 healthy people during the same period were set as the control group.The levels of adrenocorticotropic hormone(ACTH) and COR among three groups were compared.The diagnostic value of ACTH and COR levels in AECOPD, and correlation between ACTH and COR levels and severity of AECOPD were analyzed. ResultsThe serum levels of ACTH and COR in the AECOPD group, stable COPD group and control group gradually decreased, and the differences of which among three groups were statistically significant(P < 0.01).The value of ACTH combined with COR in the parallel diagnosis of AECOPD(AUC=0.897) was better than that of the singe ACTH or COR(AUC=0.715 or 0.823) and tandem diagnosis of the two(AUC=0.837).The serum levels of ACTH and COR in the mild, moderate and severe AECOPD group were gradually up-regulated, and the differences of which among three groups were statistically significant(P < 0.01).The levels of ACTH and COR were positively correlated with the severity of the disease(r=0.532, 0.467, P < 0.05). ConclusionsThe serum levels of COR and ACTH in patients with AECOPD are abnormally high, and the combination of the two is of better value in the diagnosis of AECOPD, and it is positively correlated with the severity of patient's disease.
2022, 47(2): 195-197.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.014
Abstract:
ObjectiveTo compare the application effects between intraosseous access and central venous access in the early emergency treatment of critically ill patients. MethodsAccording to the random number method, 60 critically ill patients were divided into the intraosseous group and central vein group(30 cases in each group).The intraosseous access and central vein access were established for infusion or drug treatment in the intraosseous group and central vein group, respectively.The infusion-related indexes, satisfaction of operator and safety indexes were compared between the two groups. ResultsThe first puncture success rate in intraosseous group was higher than that in central vein group(P < 0.05).The access establishment time and rescue room stay time in intraosseous group were significantly higher than those in central vein group(P < 0.01).The satisfaction of operator on equipment use and global operation in intraosseous group were significantly higher than those in central vein group(P < 0.01).There was no 1 h-access complications in the two groups. ConclusionsThe intraosseous access was an efficient, fast and safe vascular access, which can win time for the rescue of critically ill patients.
2022, 47(2): 198-201.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.015
Abstract:
ObjectiveTo explore the clinical significance of albumin in improving coagulation function and serum protein of premature infants. MethodsOne hundred premature infants were divided into the combined group and control group according to the random number table method.The control group was treated with peripheral vein nutrition combined with early micro enteral feeding, and the combined group was treated with albumin based on the control group.The nutritional recovery of preterm infants was compared between two groups, and the indicators of coagulation function between two goups after treatment were compared.The serum levels of total protein, hemoglobin and albumin in two groups before and after treatment were observed.The blood glucose, urea nitrogen and total bilirubin levels before and after treatment were compared between two groups, and the feeding tolerance and complications in two groups were analyzed. ResultsThe time of the body mass recovering to normal and feeding time as needed in combined group were shorter than those in control group, and the daily increasing of body mass in combined group was higher than that in control group(P < 0.01).After treatment, the levels of APTT, PT and TT in combined group were lower than those in control group, and the FIB level in combined group was higher than that in control group(P < 0.01).Before treatment, the differences of the serum levels of total protein, hemoglobin, albumin, blood glucose, urea nitrogen and total bilirubin were not statistically significant between two groups(P>0.05).After treatment, the serum levels of total protein, blood glucose, urea nitrogen and total bilirubin in combined group were higher than those in control group(P < 0.01), the differences of the levels of hemoglobin and albumin between two groups were not statistically significant(P>0.05), and there was no statistical significance in the blood glucose level between before and after treatment in control group(P>0.05).The serum levels of blood glucose in combined group, and urea nitrogen, total bilirubin, blood glucose, urea nitrogen and total bilirubin in two groups after treatment were higher than those before treatment(P < 0.05 to P < 0.01).The tolerance rate of premature infant feeding in combined group was higher than that in control group, and the total incidence rate of complications in combined group was lower than that in control group(P < 0.01 and P < 0.05). ConclusionsAlbumin has a significant clinical effect on improving the coagulation function and serum protein of preterm infants.It can significantly improve the blood glucose, urea nitrogen and total bilirubin levels of preterm infants, and has fewer complications.
2022, 47(2): 202-206.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.016
Abstract:
ObjectiveTo explore and compare the predictive value of the ratio of lipid accumulation product(LAP) to serum ApoB/ApoA1 in polycysticovarian syndrome(PCOS) combined with metabolic syndrome(MS). MethodsA total of 136 PCOS patients were selected as the research objects.According to the diagnosis of MS, patients were divided into the combined group and non-combined group.Physical indexes, glycolipid metabolism indexes and endocrine related indexes were collected to calculate the ratio of LAP to serum ApoB/ApoA1.The differences between the two groups and the correlation between LAP, serum ApoB/ApoA1 ratio and other indexes were compared.The ROC curve was used to analyze the predictive diagnostic value of LAP and serum ApoB/ApoA1 ratio for PCOS combined with MS. ResultsThere was no significant difference in age, LH, PRL, E2 and TC between the two groups(P>0.05).The WC, SBP, DBP, BMI, FSH, T, FPG, FINS, HOMA-IR, TG, LDL-C, ApoB/ApoA1 and LAP in the combined group were higher than those in the non-combined group, and the HDL-C was lower than that in the non-combined group(P < 0.01).The results of correlation analysis showed that ApoB/ApoA1 and LAP were positively correlated with age, WC, SBP, DBP, BMI, FSH, PRL, E2, T, FPG, FINS, HOMA-IR, TC, TG and LDL-C(P < 0.01).The AUC of ApoB/ApoA1 and LAP were 0.780 and 0.845 respectively.The specificity and sensitivity of ApoB/ApoA1 were 50.0% and 100.0%, respectively, and the specificity and sensitivity of LAP were 67.5% and 99.0%, respectively.The AUC of PCOS combined with MS was 0.818, the specificity and sensitivity were 55.0% and 100.0%, respectively.The prediction efficiency was lower than LAP and higher than ApoB/ApoA1. ConclusionsThe ratio of LAP and serum ApoB/ApoA1 can be used as an important index to predict the diagnosis of PCOS combined with MS, and the reliability of LAP in the diagnosis of PCOS combined with MS is higher than ApoB/ApoA1.
2022, 47(2): 206-208.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.017
Abstract:
ObjectiveTo study the correlation between the platelet distribution width(PDW) and Killip gerading in the elderly patients with acute non-ST-segment elevation myocardial infarction(NSTEMI). MethodsOne hundred and fifty-eight elderly patients with acute NSTEMI were divided into 4 groups according to the Killip grading Ⅰ, Ⅱ, Ⅲ and Ⅳ of cardiac function.The clinical data of all patients were recorded, and the PDW in four groups were measured.The Spearman rank correlation was used to analyze the correlation between PDW and Killip grading in elderly patients with acute NSTEMI. ResultsThe PDW increased gradually with the increasing of Killip grading Ⅰ, Ⅱ, Ⅲ and Ⅳ(P < 0.01).The results of Spearman rank correlation analysis showed that the PDW was positively correlated with cardiac function Killip grading(rs=0.792, P < 0.01). ConclusionsThe PDW is positively correlated with Killip grading in elderly patients with acute NSTEMI, which may be one of the prognostic indexes for evaluating cardiac function.
2022, 47(2): 209-211.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.018
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ObjectiveTo investigate the effects of tacrolimus on the serum levels of CXC chemokine ligand 9(CXCL9) and CXC chemokine ligand 10(CXCL10) in patients with vitiligo. MethodsTwo hundred and forty patients with vitiligo were divided into the control group(n=120) and observation group(n=120) according to the random number table method.The control group was treated with routine treatment, while the observation group was additionally treated with tacrolimus on the basis of routine treatment for 2 months.The therapeutic effects between two groups were compared.The serum levels of CXCL9, CXCL10 and tumor necrosis factor-α(TNF-α) in two groups were measured before treatment and after 2 months of treatment.The correlations between serum levels of CXCL9 and CXCL10, and TNF-α were analyzed using Pearson linear correlation analysis.The adverse reactions in two groups were recorded. ResultsThe total effective rate in observation group was higher than that in control group(P < 0.05).After treatment, the serum levels of CXCL9, CXCL10 and TNF-α in two groups were significantly lower than those before treatment, and which in observation group was significantly lower than that in control group(P < 0.01).The results of Pearson linear correlation analysis showed that the serum levels of CXCL9 and CXCL10 were positively correlated with TNF-α(r=0.713, 0.672, P < 0.01).The differences of the incidence rates of burning, erythema, rash and pruritus between two groups were not statistically significant(P>0.05). ConclusionsCompared with conventional therapy alone, tacrolimus can further reduce the serum levels of CXCL9 and CXCL10 in patients with vitiligo, which may be an important mechanism in improving clinical efficacy, and both of them are positively correlated with TNF-α.
2022, 47(2): 212-214, 218.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.019
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ObjectiveTo detect the levels of inflammatory factors and coagulation factors in peripheral blood of patients with endometriosis, and further explore the role of inflammatory factors and coagulation factors in endometriosis and its clinical diagnostic value. MethodsA total of 216 patients diagnosed as stage Ⅲ and Ⅳ endometriosis after surgery and 117 patients with benign cysts diagnosed by pathology were divided into the endometriosis group and control group, respectively.The general clinical data, levels of inflammatory factors, coagulation factors and cancer antigen 125(CA125) in peripheral blood were compared between two groups.The levels of inflammatory and coagulation factors were compared among patients of different stages.The ROC curve was used to evaluate the diagnostic value of CA125 and fibrinogen level(FIB) alone or combined in patients with stage Ⅲ and Ⅳ. ResultsThe number of pregnancies and births in endometriosis group were higher than those in control group(P < 0.01).The neutrophil lymphocyte ratio(NLR), platelet lymphocyte ratio(PLR), D-dimer(D-D), C-reactive protein(CRP) and erythrocyte sedimentation rate in endometriosis group were higher than those in control group(P < 0.01).The prothrombin time(PT) and thrombin time(TT) in endometriosis group were shorter than those in control group(P < 0.01), and the levels of FIB and CA125 levels in endometriosis group were higher than those in control group(P < 0.01).Compared with stage Ⅲ group, the levels of PLT and PLR were higher, the PT time was shorter, and the FIB was higher in stage Ⅳ group(P < 0.05 to P < 0.01).The differences of the levels of NLR and D-D, APTT and TT between stage Ⅲ group and stage Ⅳ group were not statistically significant(P>0.05).In the diagnosis of endometriosis, the sensitivity of CA125 combined with FIB was 84.6%, the sensitivity of CA125 was 81.9%, the sensitivity of FIB was 50.4%. ConclusionsThe expression of inflammatory and coagulation factors is abnormal in the endometriosis group, and which is correlated with the stage of endometriosis.The combined detection of CA125 and FIB can improve the sensitivity of the diagnosis of endometriosis.
2022, 47(2): 215-218.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.020
Abstract:
ObjectiveTo expore the effects of the overall burden of cerebral small vessel disease(CSVD) on the prognosis of recombinant tissue plasminogen activator(rt-PA) thrombolysis in ischemic stroke. MethodsA total of 100 patients with ischemic stroke were selected as the research object, and treated with rt-PA thrombolysis.According to the daily living activity(ADL) score of 3 months after the onset of disease, the patients with total score>60 points and ≤ 60 points were divided into the good prognosis group and poor prognosis group, respectively.The basic data, National Institutes of Health stroke scale(NIHSS) and total burden score of CSVD were compared between two groups, and the multivariate logistic regression analysis was used to analyze the effects of the overall burden of CSDV on the prognosis of rt-PA thrombolysis in ischemic stroke. ResultsThere was no statistical significance in the gender, alcohol history, smoking history, hypertension, coronary heart disease and infarct location between two groups(P>0.05).The proportion of patients with age >60 years old in good prognosis group was lower than that in poor prognosis group(P < 0.01), and the infarct area, total NIHSS score and total burden score of CSVD in good prognosis group were lower than those in poor prognosis group(P < 0.01).The results of multivariate logistic regression analysis showed that the age >60 years old, infarct area >3.0 cm2, NIHSS>15 points, overall burden of CSVD >2 points were the independent risk factors of poor prognosis of rt-PA thrombolysis in ischemic stroke(P < 0.05 to P < 0.01). ConclusionsThe greater the age, infarct size, NIHSS, and overall burden of CSVD are, the worse the prognosis of ischemic stroke rt-PA thrombolysis is, and the clinical diagnosis and treatment plan can be adjusted accordingly.
2022, 47(2): 219-222, 226.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.021
Abstract:
ObjectiveTo observe the changes of serum lipopolysaccharides(LPS), Toll-like receptor 4(TLR4) and intestinal microecology in patients with hepatocellular carcinoma(HCC) after transcatheter arterial chemoembolization(TACE). MethodsEleven patients with HCC were treated with TACE, and no drug was added to affect the intestinal microecological changes before and after treatment.The serum levels of LPS and TLR4 and intestinal microecological changes were obserevd before 1 day of operation and after 1 week and 1 month of operation. ResultsThe differences of the serum levels of LPS and TLR4 in patients among before 1 day of operation and after 1 week and 1 month of operation were statistically significant(P < 0.01).The results of Bonferroni's pairwise comparative analysis showed that the level of LPS before 1 day of operation was lower than that after 1 week and 1 month of operation, and the level of TLR4 after 1 month of operation was lower than that before 1 day of operation and after 1 week of operation(P < 0.05 to P < 0.01).The results of ACE index difference analysis showed that the abundance of intestinal flora increased slightly at 1 week after operation, and then decreased in the following 1 month, which was lower than that before 1 day of operation.The results of One-way ANOVA analysis showed that the abundance of probiotic bacteria such as Bacteroides and Lachnospira were higher than that before 1 day of operation and after 1 week of operation, the abundance of Streptococcus and Lactobacillus were lower than that before 1 day of operation and after 1 week of operation, and the differences of which among three groups were statistically significant(P < 0.05). ConclusionsAfter the treatment of primary liver cancer with TACE, the intestinal microecological balance can be significantly promoted through the hepato-intestinal axis.Meanwhile, the intestinal microbial homeostasis can not only be used as an indicator for the evaluation of liver cancer after TACE, but also promote the prognosis of liver cancer.
2022, 47(2): 223-226.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.022
Abstract:
ObjectiveTo explore the clinical value of single and combined detection of neutrophil to lymphocyte ratio(NLR), platelet to lymphpcyte ratio(PLR), carcinoembryonic antigen(CEA) and alpha fetal protein(AFP) in the early diagnosis of primary hepatocellular carcinoma(HCC). MethodsThe routine blood, CEA and AFP in 95 patients with primary HCC, 90 cases with liver cirrhosis and 100 healthy people in the same period were detected.The clinical value of single and combined detection of NLR, PLR, CEA and AFP in the auxiliary diagnosis of primary HCC were analyzed using the receiver operating characteristic(ROC) curve. ResultsThe levels of NLR, PLR, CEA and AFP in primary HCC group were higher than those in cirrhosis group and control group, and the levels of NLR, PLR, CEA and AFP in cirrhosis group were also higher than those in control group(P < 0.05 to P < 0.01).The areas under the ROC curve, sensitivities and specificities of the single and combined detection of NLR, PLR, CEA and AFP in the auxiliary diagnosis of primary liver cancer were(0.827, 0.602, 0.816, 0.942 and 0.983), (81.2%, 67.2%, 78.6%, 92.5% and 96.7%) and(71.3%, 64.5%, 73.4%, 82.3% and 81.2%), respectively.The areas under the ROC curve, sensitivities and specificities of the single and combined detection of NLR, PLR, CEA and AFP in the differential diagnosis of primary liver cancer were(0.633, 0.701, 0.505, 0.717 and 0.761), (61.3%, 68.7%, 51.2%, 70.6% and 78.3%) and(68.4%, 72.6%, 56.7%, 68.7% and 73.2%), respectively. ConclusionsThe combined diagnostic tests of NLR, PLR, CEA and AFP can improve the sensitivity of auxiliary diagnosis of primary HCC, and have certain clinical application value in the differential diagnosis of liver cirrhosis.
2022, 47(2): 227-230.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.023
Abstract:
ObjectiveTo compare the diagnostic value between flow cytometry immunotyping and pathological bone marrow biopsy in bone marrow invasion of non-Hodgkin's lymphoma(NHL). MethodsFlow cytometry immunotyping and pathological bone marrow biopsy were performed in 253 cases of lymphoma.Using clinical comprehensive diagnosis as the gold standard, the sensitivity and specificity of two methods were analyzed, the different subtypes of NHL were classified, and the incidence rate of bone marrow invasion was analyzed using different detection methods. ResultsThe sensitivity of the flow cytometry in detecting the incidence rate of bone marrow invasion was 91.67%(88/96), which was higher than that of pathological bone marrow biopsy 82.29%(79/96), and the difference of which was not statistically significant(χ2=0.16, P>0.05).The specificity of both methods were 100.00%.The incidence rates of bone marrow invasion of mantle cell lymphoma using gold standard, flow cytometry and pathological bone marrow biopsy were 84.00%, 80.00% and 68.00%, respectively.The incidence rates of bone marrow invasion of diffuse large B-cell lymphoma using gold standard, flow cytometry and pathological bone marrow biopsy were 23.75%, 22.50% and 22.50%, respectively.The differences of the detection between gold standard and pathological bone marrow biopsy in chronic lymphocytic leukemia/small cell lymphoma were statistically significant(P < 0.01).The incidence rates of bone marrow invasion in mantle cell lymphoma, chronic lymphocytic leukemia/small cell lymphoma, follicular cell lymphoma, B-NHL unclassified detected by flow cytometry were higher than that of pathological bone marrow biopsy, while for T/NK cell lymphoma, the bone marrow invasion rate detected by flow cytometry was lower. ConclusionsFlow cytometry is a sensitive and efficient method in the detection of NHL.
2022, 47(2): 231-236.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.024
Abstract:
ObjectiveTo explore the differences of the bacterial species, drug resistance and inflammatory markers such as C-reactive protein(CRP), procalcitonin(PCT) and white blood cell count(WBC) between hematologic patients and intensive care patients. MethodsIn the contemporaneous area, the patients with hematological disease and intensive care patients with positive blood culture were divided into the hematological disease group(HDG) and non-hematological disease group(NHICG), respectivlely, and the patients with hematological disease and intensive care patients with negatvie blood culture were divided into the control group(HDCG) and negative control group(NCG), respectively.The levels of the PCT, CRP and WBC in all cases were detected.The patients with hematologic diseases were subdivided into the acute lymphoblastic leukemia group(ALLG group), acute lymphoblastic leukemia group(AMLG group) and aplastic anemia group(AAG group).The differences of the blood culture identification, drug sensitivity test results and inflammatory indexes among groups and subgroups were observed. ResultsComparison of the total positive rates between HDG and NHICG groups: the top three were coagulase-negative staphylococcus, Klebsiella pneumoniae and subspecies, and Escherichia coli, with only different sequencing.The bacteria in two groups were mainly Gram-negative bacteria.The positive rate, number of drug-resistant strains and bacteria in the top of drug-resistant rate were still roughly the same in the HDG and NHICG groups.Except NLR, the differences of other inflammatory indicators among HDG, HDCG, NHICG and NCG groups were statistically significant(P < 0.01).The differences of all inflammatory indicators among ALLG, AMLG and AAG subgroups were not statistically significant(P>0.05). ConclusionsThe detection rate and drug resistance rate of bloodstream infection bacteria in patients with hematological diseases and severe patients are relatively concentrated.The types and drug-resistant types of bloodstream infection bacteria in the three common blood diseases are roughly the same.The methicillin-resistant coagulase-negative staphylococcus has become a common Gram-positive pathogenic bacteria and multi-drug resistant bacteria in blood patients and severe patients.The combination of PCT, CRP, WBC and other inflammatory markers is of certain significance for the rapid and effective identification of bloodstream infection.
2022, 47(2): 236-241.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.025
Abstract:
ObjectiveTo understand the research status, sort out its research content and development context, and point out the research deficiency and development trend in the field of postpartum depression. MethodsUsing China National Knowledge Infrastructure(CNKI) as the data source, 2 479 articles were analyzed using CiteSpace5.6R5. ResultsThe research process of postpartum depression in China can be divided into the preliminary understanding stage, development stage and gradual deepening stage, and the treatment of postpartum depression and test of efficacy of postpartum depression were the key research contents.The neurophysiological mechanism of postpartum depression and pathogenic mechanism of psychosocial factors were research trend, and the lack of academic exchange and cooperation consciousness were the deficiency of study.The interdisciplinary research should be strengthened, the research methods should be innovated, and a variety of diagnostic tools should be developed. ConclusionsAlthough the abundant academic achievements have been made in the study of postpartum depression in China, there are few researches on the pathogenesis and genetics.Therefore, the academic exchanges and cooperation should be strengthened to promote the research of postpartum depression to achieve breakthrough.
2022, 47(2): 242-248.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.026
Abstract:
ObjectiveTo evaluate the efficacy of ambroxol combined with budesonide aerosol inhalation in the treatment of neonatal pneumonia. MethodsThe relevant literature informations were searched from the establishment of the database to September 2019 of PubMed, EMbase, Cochrane, CBM, CNKI, Wanfang Med Online and VIP Journal Resource Network.All relevant randomized controlled trials(RCT) comparing ambroxol aerosol inhalation alone with ambroxol combined with budesonide aerosol inhalation in the treatment of neonatal pneumonia were collected.RevMan5.3 software was used for Meta-analysis, and the Stata 12.0 software was used to evaluate the risk of quality bias. ResultsA total of 2 124 patients from 16 RCT were included.The results of Meta-analysis showed that the ambroxol combined with budesonide aerosol inhalation in reducing cough disappearance time, expiratory dyspnoea disappearance time, dyspnea disappearance time, pulmonary rales disappearance time, length of hospital stay and overall response rate were better than those of ambroxol aerosol inhalation alone(P < 0.01). ConclusionsAmbroxol combined with budesonide aerosol inhalation in the treatment of neonatal pneumonia has significant effects, and can shorten the time of outcome indicators.
2022, 47(2): 249-253, 258.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.027
Abstract:
ObjectiveTo investigate the improvement of C-type behavior patterns of staged cognitive intervention in perioperative lung cancer patients with protective medicine. MethodsNew lung cancer patients and family caregivers in Department of Thoracic Surgery of The First Affiliated Hospital of University of Science and Technology of China between June 2018 and December 2018 were enrolled as the study subjects.Forty lung cancer patients and 40 caregivers, 40 patients and 40 last effective cases were divided into the final intervention group, intervention group and control group, respectively.The C-type behavior patterns among 3 groups were compared before and after intervention. ResultsThere was no statistical significance in the scores of subscales of C-type behavior pattern before intervention among three groups at admission(P > 0.05), and the differences of the anxiety, depression, anger, optimist and social support of C-type behavior patterns among three groups were statistically significant after intervention(P < 0.05 to P < 0.01).After intervention, the differences of anger, intrinsic anger, extrinsic anger, reason and control of C-type behavior patterns among three groups were not statistically significant(P > 0.05), the decreasing degree of anxiety, depression, anger inward and anger scores in the control group, intervention group and final intervention group were gradually increased(P < 0.05 to P < 0.01), and the increasing degree of optimist and social support scores in the control group, intervention group and final intervention group were gradually increased(P < 0.05 to P < 0.01). ConclusionsThe staged cognitive intervention in improving the C-type behavior patterns of perioperative lung cancer patients with protective medicine is effective, and the effects of family caregivers synchronous cognitive intervention is more better than that of patients cognitive intervention only.
2022, 47(2): 254-258.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.028
Abstract:
ObjectiveTo explore the effects of hierarchical management based on FRAX risk assessment on the disease cognition and health-related behaviors of community elderly patients with osteoporosis. MethodsA total of 126 elderly patients with osteoporosis from June 2017 to July 2018 in community of Huangpu district Shanghai were selected by stratified random sampling method.According to the time, the patients were divided into the observation group(from January to July 2018, 64 cases) and control group(from June to December 2017, 62 cases).Two groups were given the same health management content.The control group was given health education manual, centralized education, individual guidance, social support, follow-up management and other routine nursing intervention.The observation group was additionally given the hierarchical management based on FRAX risk assessment on the basis of control group.Two groups were followed up for 12 months.The disease cognition level, health related behavior, 25 hydroxyvitamin D(25OHD) and bone mineral density(BMD) levels and incidence rates of fall and fracture were compared between two groups. ResultsAfter 12 months of following-up, the total scores of osteoporosis risk factors, exercise knowledge, calcium knowledge and disease cognition in observation group were higher than those in control group, the total scores of diagnosis and treatment-related behavior, nutrition-related behavior, exercise-related behavior and health-related behavior in observation group were higher than those in control group, and the levels of 25OHD and BMD in lumbar 3-4 and femoral neck in observation group were higher than those in control group(P < 0.05 to P < 0.01).The incidence rates of fall and fracture in observation group(6.45%) were lower than those in control group(18.97%) (P < 0.05). ConclusionsHierarchical management based on FRAX risk assessment can help to improve the cognitive level of disease, promote the formation of health-related behaviors, improve vitamin D and BMD levels, prevent falls and fractures in community elderly patients with osteoporosis.
2022, 47(2): 259-264.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.029
Abstract:
ObjectiveTo investigate the current status of emotional labor and work engagement of emergency nurses, explore the correlation between the two, and analyze the factors affecting work engagement. MethodsFrom February to March 2019, 284 nurses in emergency departments from 20 public hospitals in Anhui province were selected using the convenience sampling method, and the nurses were investigated using the general data questionnaires, emotional labor questionnaire and Utrecht work engagement scale.The Pearson correlation analysis was used to analyze the correlation between the two, and the hierarchical regression analysis was used to analyze the factors affecting work engagement. ResultsThe total score of emotional labor, average score of emotional labor, surface acting score, deep acting score, emotional expression requirement score and total work engagement score in 284 emergency nurses were(38.74±8.21), (2.76±0.59), (24.49±7.03), (14.25±2.93), (14.31±4.72) and(62.62±19.30) points, respectively.The scores of vitality, dedication and concentration in 284 emergency nurses were(24.90±8.25), (18.01±5.71), and(19.71±6.69) points, respectively.There was a positive correlation between emotional labor and work engagement in emergency nurses(P < 0.01).The level of work engagement of emergency formal nurses was higher than that of contract nurses(P < 0.01).The deep acting dimension of emotional labor could independently explain the 14.8% of total variation of work engagement of nurses in emergency department(P < 0.01). ConclusionsBoth emotional labor and work engagement of emergency nurses are at medium level.The nursing managers should fully realize the importance of emotional labor, and take targeted training for emergency nurses from the perspective of deep acting, so as to improve their work engagement level and mobilize the energy, dedication and concentration of emergency nurses.
2022, 47(2): 265-267.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.030
Abstract:
ObjectiveTo explore the effects of the depth of sedation and duration of deep sedation on occurrence of delirium in patients with severe acute pancreatitis(SAP). MethodsThe clinical data of 258 patients with SAP were retrospectively analyzed.The end of treatment in ICU of patients was set as the study endpoint, the patients were divided into the delirium group and non-delirium group.The depth of sedation and duration of deep sedation between two groups were compared. ResultsThe depth of sedation and duration of deep sedation in delirium group were greater than those in non-delirium group(P < 0.01). ConclusionsThe deeper sedation depth and the longer sedation time in ICU patients with SAP, the higher the incidence rate the delirium is.
2022, 47(2): 268-270.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.031
Abstract:
ObjectiveTo explore the application effects of comprehensive nursing intervention measures during peri-catheterization of peripherally inserted central catheter(PICC) in elderly patients with non-small cell lung cancer. MethodsFifty-six non-small cell lung cancer patients during peri-catheterization of PICC were randomly divided into the observation group and control group(28 cases in each group).The control group was treated with routine nursing intervention, and the observation group was treated with comprehensive nursing intervention.The anxiety and depression scores, quality of life, satisfaction, health knowledge and treatment compliance were compared between two groups. ResultsThe scores of anxiety and depression in observation group were lower than those in control group(P < 0.01).The score of quality of life in observation group was better than that in control group(P < 0.01).The health knowledge and treatment compliance in observation group were significantly higher than those in control group(P < 0.01 and P < 0.05).There was no statistical significance in the incidence rate of complications between two groups(P > 0.05). ConclusionsThe application of comprehensive nursing intervention in elderly patients with non-small cell lung cancer during PICC pericatheterization therapy has ideal effect, which can effectively reduce the adverse emotional impact and promote the improvement of quality of life of elderly patients.
2022, 47(2): 271-273.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.032
Abstract:
ObjectiveTo investigate the effects of self-made constant pressure stomach lavage device for newborn on neonatal gastric lavage. MethodsOne hundred and twenty children with neonatal hypopharyngeal syndrome were randomly divided into the observation group and control group(60 cases in each group).The control group was treated with traditional syringe gastric lavage, while the observation group was treated with constant pressure stomach lavage device for newborn modified by disposable sputum collector for gastric lavage.The effects of gastric lavage and damage of gastric mucosa were compared between the two groups. ResultsThe relief time of vomiting, disappearance time of gastric retention and recovery time of gastrointestinal feeding in observation group were significantly shorter than those in control group(P < 0.01).The effective rate in observation group was significantly higher than that in control group(P < 0.01), and the undamaged rate of gastric mucosa in observation group was higher than that in control group(P < 0.01). ConclusionsIn clinical gastric lavage for newborns, the constant pressure stomach lavage device for newborns modified by disposable sputum collector has high effectiveness and safety.
2022, 47(2): 274-277.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.033
Abstract:
ObjectiveTo analyze the preoperative visit needs of patients treated with cervical carcinoma radical surgery, and its influencing factors. MethodsA self-designed questionnaire was used to investigate 218 patients treated with cervical carcinoma radical surgery in a grade A tertiary hospital in Qingdao city. ResultsThe total score of preoperative visit needs of patients treated with cervical carcinoma radical surgery was (155.34±22.31) points, the score of psychological support dimension was the highest, followed by hospitalization expense dimension, and the scores of environment information and surgical procedure dimension were the lowest.The results of multiple linear regression analysis showed that education level, age, and type of medical insurance were the factors influencing the preoperative visit needs of patients treated with cervical carcinoma radical surgery(P < 0.05 to P < 0.01). ConclusionsThe preoperative visit needs of patients treated with cervical carcinoma radical surgery are high.It is suggested that the nursing visitors should give more individualized and accurate preoperative guidance according to the specific conditions of the patients, pay attention to the application of psychological intervention measures, reduce the stress response of patients, and improve the satisfaction of patients.
2022, 47(2): 278-280.
doi: 10.13898/j.cnki.issn.1000-2200.2022.02.034
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ObjectiveTo observe the intervention effects of simplified finger exercise on the radial artery occlusion and related complications after coronary angiography(CAG). MethodsA total of 326 patients treated with transradial CAG were randomly divided into the observation group(163 cases) and control group(163 cases).The observation group and control group were given the routine care combined with simplified finger exercise and routine care, respectively.The palm-circumference diameter, degree of numbness and pain, and incidence rate of radial artery occlusion after 24 h and 30 d of operation were observed. ResultsThe degree of postoperative limb pain, numbness and swelling in observation group were lower than those in control group(P < 0.05 to P < 0.01).The incidence rate of radial artery occlusion in observation group after 24 h of operation was lower than that in control group(P < 0.05), and there was no statistical significance in the bleeding degree and incidence rate of radial artery occlusion after 30 d of operation between two groups(P > 0.05). ConclusionsThe simplified finger exercise can reduce limb numbness, pain and swelling after CAG via radial artery, and reduce the incidence rate of radial artery occlusion after 24 h of surgery.
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