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2022 Vol. 47, No. 3
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2022, 47(3): 281-284.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.001
Abstract:
ObjectiveTo compare the effects of incomplete Freund′s adjuvant(IFA) and complete Freund′s adjuvant(CFA) combined with calf Ⅱ collagen(CⅡ) in establishing the collagen-induced rheumatoid arthritis(CIA) rat model. MethodsThirty SD rats aged 8 weeks were randomly divided into the 0.9% sodium chloride solution group(control group), IFA+CⅡ group and CFA+CⅡ group(10 rats in each group).The IFA/CFA and CⅡ were blerding in equal volume, and intradermally injected into the root of rat tail.The 200 μL and 100 μL mixture were injected into rats in the model group on day 0 and day 7, respectively.The same volume of 0.9% sodium chloride solution was injected into rats in the control group using the same method.The scores of arthritis index(AI) in three groups were recorded every 7 days from the initial immunization.HE staining was performed in ankle joints of 5 rats from three groups on day 14 to observe the pathological changes. ResultsOn day 0 and day 7 after modeling, the AI values in three groups were 0.On day 14 after initial immunization, the AI values in IFA+CⅡ group and CFA+CⅡ group were(6.5±0.5) and(7.4±0.4), respectively, and the difference of which was statistically significant(P < 0.01).On day 21 after modeling, the AI values in IFA+CⅡ group and CFA+CⅡ group were(8.8±0.5) and(7.8±0.8), respectively, and the difference of which was statistically significant(P < 0.01).But the difference of AI values in IFA+CⅡ group and CFA+CⅡ group were not statistically significant on day 14 and day 21 after modeling(P>0.05).The results of HE staining of the ankle joint showed that the cartilage surface in ankle joint in the IFA+CⅡ group was rough and slightly damaged, and inflammatory cell infiltration was observed in the articular cavity.In CFA+CⅡ group, the articular cartilage was obviously damaged, presented saw tooth shape, and had obvious pannus and a large amount of inflammatory cell infiltration. ConclusionsBoth IFA+CⅡ and CFA+CⅡ can be used to prepare RA model, and the inflammatory response of IFA+CⅡ is slight, which is the preferred method for preparing RA model.
2022, 47(3): 284-288.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.002
Abstract:
ObjectiveTo investigate the effect of X-linked inhibitor of apoptosis protein(XIAP) gene on the tumor necrosis factor-α(TNF-α)-induced apoptosis, and expression of vascular endothelial growth factor(VEGF) and cyclooxygenase-2(COX-2) in nasopharyngeal carcinoma cells. MethodsHuman nasopharyngeal carcinoma 5-8F cells were divided into blank group, TNF-α group(cells treated with 10 ng/mL exogenous TNF-α for 12 h), si-XIAP group(cells transfected with si-XIAP for 48 h) and TNF-α+si-XIAP(cells transfected with si-XIAP for 48 h, then treated with 10 ng/mL exogenous TNF-α for 12 h).Western blotting was used to detect the protein expression of XIAP, VEGF, COX-2, Cleaved caspase3, Bax, NF-κB p65 and Ikkβ.MTT assay and flow cytometry were used to detect cell viability and apoptosis rate. ResultsThe expression of XIAP in 5-8F cells transfected with si-XIAP was significantly lower than that in blank group(P < 0.01).The cell viability in TNF-α group and si-XIAP group was lower than that in blank group(P < 0.05), and the apoptosis rate and the expression of Cleaved caspase3 and Bax were higher than those in blank group(P < 0.05).The expressions of VEGF, COX-2, NF-κB p65 and Ikkβ in TNF-α group were higher than those in blank group(P < 0.05).The expressions of VEGF, COX-2, NF-κB p65 and Ikkβ in si-XIAP group were lower than those in blank group(P < 0.05).The cell viability in TNF-α+si-XIAP group was lower than that in TNF-α group and si-XIAP group(P < 0.05);the expressions of VEGF, COX-2, NF-κB p65 and Ikkβ were lower than those in TNF-α group and higher than those in si-XIAP group(P < 0.05);the apoptosis rate and the expression of Cleaved caspase3 and Bax were higher than those in TNF-α group and si-XIAP group(P < 0.05). ConclusionsDown-regulating the expression of XIAP gene enhances TNF-α-induced apoptosis and reduces the expression of VEGF and COX-2 in nasopharyngeal carcinoma 5-8F cells, which is related to the inhibition of NF-κB signaling pathway.
2022, 47(3): 289-292.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.003
Abstract:
ObjectiveTo study the activation of ferroptosis by resveratrol in colorectal cancer SW480 cells, and to explore its possible mechanism. MethodsAfter intervening SW480 cells with different doses of resveratrol(0, 10, 20, 40, 80, 160 μmol/L) for 24, 48 and 72 h, the effect of resveratrol on cell proliferation inhibition rate was analyzed by CCK8 method.After intervening SW480 cells with different doses of resveratrol(0, 20, 40, 80 μmol/L) for 48 h, Western blotting was used to analyze the expression levels of cystine/glutamate antiporter system Xc(xCT), glutathione peroxidase 4(GPX4) and divalent metal transporter 1(DMT1), and flow cytometry was applied to detect the change of reactive oxygen specie(ROS) level which was indicated by fluorescence intensity. ResultsAfter SW480 cells were treated with resveratrol, the inhibition rate of cell proliferation was significantly increased, which showed a time- and concentr ation-dependent manner(P < 0.05);the expression level of xCT and GPX4 protein were significantly decreased, and the expression level of DMT1 was greatly increased(P < 0.05);resveratrol also induced the accumulation of ROS in SW480 cells(P < 0.05). ConclusionsResveratrol increases the accumulation of ROS by inhibiting the expression of GPX4 and xCT protein to induce ferroptosis in SW480 cells, which may involve the activation of DMT1 expression.
2022, 47(3): 293-296.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.004
Abstract:
ObjectiveTo investigate the effects of asiaticoside on the growth, apoptosis, apoptosis pathway in human nasopharyngeal carcinoma cell line CNE-2. MethodsThe CNE-2 cells were cultured in vitro, and treated with different concentrations of Asiaticoside.The proliferation of cells were detected using MTT and CCK-8 assay, and the apoptosis rate of cells was detected using flow cytometry.The expression levels of Vimentin, NLRP3, Bcl-2 and Caspase-3 protein were determined using Western blotting, and the serum levels of TNF-α and IL-1β were determined using ELISA. ResultsThe results of MTT and CCK-8 assay showed that asiaticoside could inhibit the proliferation of CNE-2 cells ina concentration-dependent manner, the results of flow cytometry showed that asiaticoside could promote the apoptosis of CNE-2 cells in a concentration-dependent manner, the results of Western blotting showed that asiaticoside promoted the expression of Caspase-3, and inhibited the expression levels of Vimentin, NLRP3 and Bcl-2, and the results of ELISA showed that the contents of TNF-α and IL-1β could be decreased in a concentration-dependent manner. ConclusionsAsiaticoside can significantly promote the apoptosis of human nasopharyngeal carcinoma cell line CNE-2, and the mechanism may be realized by down-regulating the expression of Vimentin/NLRP3 protein.
2022, 47(3): 297-301.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.005
Abstract:
ObjectiveTo observe the correlation between sleep disturbance and ecological executive function after acute ischemic stroke. MethodsA total of 103 patients with acute ischemic stroke were selected and divided into sleep disturbance group and normal sleep group according to the Pittsburgh sleep quality index scores.Polysomnography(PSG) and ecological behavior rating inventory of executive function-adult version(BRIEF-A) were used to evaluate sleep quality and executive function, and the difference of which between the two groups was compared.Multiple stepwise regression analysis was applied to explore the correlation between PSG sleep parameters and BRIEF-A scores. ResultsThe total score(GEC), scores of metacognition index(MI) and its factors(organization, planning, working memory, task initiation, task monitoring), and scores of behavior regulation index(BRI) and its factors(conversion, inhibition, self-monitoring, emotional control) of BRIEF-A in sleep disturbance group were higher than those in normal sleep group(P < 0.05 to P < 0.01).Compared with the normal sleep group, the total sleep time(TST) was shortened, the sleep efficiency(SE) decreased, the non-rapid eye movement sleep phase 3(NREM3) decreased, and the rapid eye movement sleep latency(RL), sleep latency(SL), wake-up time after falling asleep, proportion of non-rapid eye movement sleep phase 1 to total sleep time, and proportion of rapid eye movement sleep phase to total sleep time(REM%) increased in the sleep disturbance group(P < 0.05 to P < 0.01).Multiple stepwise linear regression analysis showed that GEC, MI, BRI were negatively correlated with TST and SE(P < 0.05 to P < 0.01), GEC and MI were negatively correlated with the proportion of NREM3 to total sleep time(P < 0.01 and P < 0.05), GEC and MI were significantly positively correlated with REM%(P < 0.01), and GEC was significantly positively correlated with REM%(P < 0.01). ConclusionsPatients with sleep disturbance after acute ischemic stroke have ecological executive function compared with those without sleep disturbance, which are related to sleep parameters in the sleep-wake cycle.
2022, 47(3): 302-304.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.006
Abstract:
ObjectiveTo observe the expression level of costimulatory molecules CD28 and ICOS on the surface of peripheral blood T lymphocyte in patient with chronic hepatitis B, and to explore the significance of CD28 and ICOS in chronic hepatitis B virus infection. MethodsFifty-six patients with chronic hepatitis B were selected as the observation group, and 20 healthy examinees in the same period were included as the control group.Flow cytometry was used to detect the expression level of CD28 and ICOS on the surface of T lymphocyte in the subjects′ peripheral blood. ResultsThere were no significant differences in the peripheral blood CD4+, CD8+T lymphocytes and the ratio of CD4+/CD8+ between the two groups(P>0.05).The levels of CD4+/CD28+T cells and CD8+/CD28+T cells in the peripheral blood in observation group were significantly lower than those in control group(P < 0.01).There was no significant difference in the level of CD4+ICOS+T cells between the two groups(P>0.05), and the level of CD8+ICOS+T cells in observation group was significantly higher than that in control group(P < 0.01). ConclusionsPatients with chronic hepatitis B virus infection have both immunodeficiency and immune disorder, which are mainly related to the abnormal expression of the costimulatory molecules CD28 and ICOS on the surface of T lymphocyte.
2022, 47(3): 305-309.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.007
Abstract:
ObjectiveTo investigate the incidence and main risk factors of contrast-induced nephropathy(CIN) after emergency percutaneous coronary intervention(PCI) in patients with acute myocardial infarction(AMI), and establish a quantitative nomogram risk prediction model to guide clinical application. MethodsA total of 1 417 patients with AMI undergoing PCI were selected as the study subjects, and the patients were divided into CIN group(n=180) and non-CIN group(n=1 237).Univariate and multivariate logistic regression analysis were used to screen the main risk factors, and a nomogram model was established.The receiver operating characteristic(ROC) curve was applied to analyze the predictive efficacy, and Hosmer-Lemeshow test was used to calculate the goodness of fit.According to the nomogram score, the patients were divided into low-risk, medium-risk and high-risk group, and the incidence of CIN was compared among the groups. ResultsCompared with the non-CIN group, the patients in the CIN group with age >75 years old increased, the level of diastolic blood pressure, preoperative serum creatinine, total bilirubin, globulin, creatine kinase isoenzyme and C-reactive protein increased, and the left ventricular ejection fraction(LVEF) < 40%, glomerular filtration rate(eGFR) < mL·min-1·1.73 m-2, metformin application rate and contrast dosage >300 mL increased, but the operation time, number of target vessels, uric acid, urea nitrogen, albumin, low density lipoprotein and fasting blood glucose decreased, and the differences of which were statistically significant(P < 0.05 to P < 0.01).Multivariate regression analysis showed that LVEF < 40%, eGFR < mL·min-1·1.73 m-2 and contrast dosage >300 mL were the main risk factors for CIN(P < 0.01).The predictive efficacy of nomogram by ROC curve analysis was 0.867, which was significantly higher than that of the internal validation Mehran score(0.712)(P < 0.01).The calibration curve showed that the predicted results were in good agreement with the actual results.The incidence of CIN in high-risk patients was significantly higher than that in medium-risk patients, and the incidence of CIN in medium-risk patients was significantly higher than that in low-risk patients(P < 0.01). ConclusionsAMI patients have a higher incidence of CIN after emergency PCI.LVEF < 40%, eGFR < mL·min-1·1.73 m-2 and contrast dosage >300 mL are the main risk factors.The quantitative nomogram risk model has a good predictive efficacy and is worthy of application.
2022, 47(3): 309-312.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.008
Abstract:
ObjectiveTo detect the levels of chemokine ligand-2(CCL2) and interleukin 6(IL-6) in peripheral blood in amyotrophic lateral sclerosis(ALS) patients, and analyze the correlation of the levels of CCL2 and IL-6 with cognitive function. MethodsThirty-two ALS patients and 20 myopathy patients were divided into the observation group and control group, respectively.The plasma levels of CCL2 and IL-6 in two groups were detected using ELISA, and the cognitive function in two groups were evaluated using Mini-mental State Examination(MMSE) and Wechsler Intelligence Scale(WMS). ResultsThe differences of the age, gender and education level between two groups were not statistically significant(P>0.05).The results of ELISA showed that the levels of CCL2 and IL-6 in observation group were higher than those in control group(P < 0.01).The MMSE score in observation group was lower than that in control group(P < 0.01).The scores of the memory quotient, long-term memory, short-term and transient memory in observation group were lower than those in control group(P < 0.05 to P < 0.01).The level of CCL2 was negatively correlated with the MMSE score and WMS score(P < 0.05). ConclusionsCompared with the myopathy patients, the plasma levels of CCL2 and IL-6 are upregulated, and the cognitive function decrease in ALS patients.The level of CCL2 in peripheral blood is negatively correlated to the cognitive function of patients.
2022, 47(3): 313-316.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.009
Abstract:
ObjectiveTo analyze the clinical features of maintenance hemodialysis patients complicated with hypotension. MethodsThe clinical data of 127 chronic renal failure patients treated with maintenance hemodialysis were retrospectively analyzed.According to the presence or absence of hypotension during hemo dialysis, the patients were divided into the occurrence group and non-occurrence group.The clinical indicators were compared between two groups, and the influencing factors of hypotension in hemodialysis patients were analyzed. ResultsAmong 127 patients, 67 cases(52.75%) with hypotension during hemo dialysis were found.A total of 8 255 times hemo dialysis in 127 patients were performed, and the hypotension occurred in 1 125 times(13.63%).The differences of the body mass index, left ventricular ejection fraction, hematocrit, hemoglobin, serum albumin and total serum protein between two groups were not statistically significant(P>0.05), and the differences of the age, ultrafiltration volume and ratio of ultrafiltration volume to body mass between two groups were statistically significant(P < 0.05 to P < 0.01).The results of logistic regression analysis showed that the age, ultrafiltration volume and ratio of ultrafiltration volume to body mass were the independent risk factors for hypotension in hemodialysis patients(P < 0.01). ConclusionsHypotension is a common complication of maintenance hemodialysis patients.The age, ultrafiltration volume, and ratio of ultrafiltration volume to body mass are the independent risk factors for hypotension in hemodialysis patients.In clinical treatment, the elderly patients and patients with high ultrafiltration volume should be closely observed, and the preventive measures should timely be taken.
2022, 47(3): 316-318.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.010
Abstract:
ObjectiveTo investigate the adverse reactions and long-term effects of different adjuvant therapies for stageⅠb1-Ⅱa2 cervical cancer with intermediate and high risk factors after radical surgery. MethodsA total of 128 patients with stageⅠb1-Ⅱa2 cervical cancer were analyzed retrospectively, 40 patients of which received combination of surgery, adjuvant radiotherapy and cisplatin(DDP) weekly(radiotherapy combined with DDP group), and 88 patients of which received combination of surgery, adjuvant radiotherapy, and paclitaxel combined with ODP(TP) regimen(radiotherapy combined with TP group).The adverse reactions and long-term effects of the two groups were compared. ResultsCompared with radiotherapy combined with TP group, the level of grade Ⅲ-Ⅳ leucocytes decreased in radiotherapy combined with DDP group(P < 0.01);the level of grade Ⅲ-Ⅳ hemachrome and grade Ⅲ-Ⅳ platelet, and incidence of grade Ⅲ-Ⅳ vomiting and grade Ⅲ-Ⅳ adiation proctitis in radiotherapy combined with DDP group reduced, but the differences of which were not statistically significant(P>0.05).There was no significant difference in the follow-up time, rate of recurrence and 5-year survival rate between the two groups(P>0.05). ConclusionsThe weekly radiotherapy combined with DDP therapy for stageⅠb1-Ⅱa2 cervical cancer patients with intermediate and high risk factors after radical surgery has little adverse reactions and good long-term efficacy.
2022, 47(3): 319-321.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.011
Abstract:
ObjectiveTo explore the clinical application of a new minimally invasive external fixation stent in the treatment of rib fractures(fracture number < 3). MethodsThe clinical data of 24 patients with rib fractures(fracture number < 3)were analyzed.The patients were randomly divided into the observation and control group.The observation group was treated with new minimally invasive external fixation stent, while the control group was treated with conservative method.The analgesic drug dosage, chest pain VAS score and complications were compared between two groups. ResultsThe analgesic drug dosage in observation group after 15d of operation was significantly lower than that in control group(P < 0.05).The VAS scores in two gorups after 5, 10 and 15d of operation significantly decreased, and the postoperative VAS scores in two groups were significnatly lower than that before opertaion(P < 0.01).The VAS scores in observation group were lower than those in control group after 5 and 10d of operation(P < 0.05).No local infection, pulmonary infection, hemopneumothorax occurred in two groups. ConclusionsThe treatment of rib fractures(fracture number < 3)with new minimally invasive external fixation stent is effective with small trauma, which can relieve the pain of patients, and reduce the dosage of analgesic drug.
2022, 47(3): 322-326.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.012
Abstract:
ObjectiveTo investigate the expression levels and clinical significance of tumor necrosis factor receptor-associated factor 4(TRAF4) in colon cancer. MethodsThe expression levels of TRAF4 in 112 colon cancer tissue specimens and their matched adjacent tissue specimens were detected using the immunohistochemical staining.According to the staining results, the patients were divided into the high expression group and low expression group, and the clinical pathology data and survival prognosis between two groups were compared.The Kaplan-Meier method was used for survival analysis, and the Log-rank test was used to compare the survival rates between groups.The COX proportional hazards regression model was used to analyze the prognostic factors of colon cancer patients. ResultsTRAF4 was mainly expressed in the nucleus.The high expression rate of TRAF4 in colon cancer tissue was 73.21%(82/112), which was significantly higher than that in the adjacent tissues[33.04%(37/112)](P < 0.01).The high expression rate of TRAF4 in patients with poorly differentiated colon cancer was significantly higher than that in patients with well differentiated patients(P < 0.01).The higher the T stage, the greater the high expression rate of TRAF4 was(P < 0.01).The high expression rate of TRAF4 in patients with stage M1 colon cancer was significantly higher than that in patients with M0 stage(P < 0.01).The degree of differentiation, T stage, N stage, M stage and TRAF4 were the independent factors affecting the overall survival time of colon cancer patients(P < 0.05), and the degree of differentiation, N stage, M stage and TRAF4 were the independent factors influencing the progression-free survival time of colon cancer patients(P < 0.05).The overall survival rate and progression-free survival rate in high expression group were 64.29% and 69.64%, respectively, which were lower than those in low expression group(82.43% and 90.18%)(P < 0.05).The median survival time and progression-free survival time in the high expression group were 70.92 months and 72.55 months, respectively, which were lower than those in low expression group(84.81 months and 89.66 months)(P < 0.05). ConclusionsTRAF4 is related to the degree of differentiation, T stage and M stage of colon cancer, and the high expression of TRAF4 predicts the poor survival prognosis of colon cancer.
2022, 47(3): 326-329.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.013
Abstract:
ObjectiveTo analyze the clinical characteristics and related risk factors of frailty syndrome in elderly patients with chronic heart failure(CHF). MethodsA total of 184 patients with frailty syndrome from January 2017 to March 2019 were selected as the study object. The clinical characteristics and related risk factors of frailty syndrome were analyzed by the questionnaires and medical records. ResultsAmong 184 elderly patients with CHF, the frailty syndrome in 66 cases were found, and the incidence rate of which was 35.87%(66/184). The female, age, junior high school education or below, solitary and grade Ⅲ+Ⅳ of the New York Heart Association(NYHA classification), complicated diseases(2-7 kinds) and creatinine level in frailty syndrome group were higher than those in non-frailty syndrome group, and the levels of hemoglobin, albumin and left ventricular ejection fraction in frailty syndrome group were lower than those in non-frailty syndrome group(P < 0.05 to P < 0.01). The results of logistic multivariate regression analysis showed that the age, number of complicated diseases, and levels of hemoglobin, albumin and left ventricular ejection fraction were the independent influencing factors of frailty syndrome in elderly CHF patients(P < 0.05 to P < 0.01). ConclusionsThe risk of frailty syndrome in elderly CHF patients is high, and whcih can be affected by many factors. The management intervention should be strengthened for elderly CHF patients with advanced age and multiple diseases to reduce the occurrence of frailty syndrome.
2022, 47(3): 330-333.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.014
Abstract:
ObjectiveTo compare the clinical effects of three different postoperative analgesic regimens in laparoscopic total hysterectomy. MethodsNinety patients scheduled by laparoscopic total hysterectomy were randomly divided into the dezocine combined with tramadol group(DT group), dezocine combined with flurbiprofen axetil group(DF group) and dezocine combined with sufentanil group(DS group)(30 cases in each group). The DT, DF and DS groups were treated with 0.5 mg/kg dezocine+10 mg/kg tramadol, 0.5 mg/kg dezocine+3 mg/kg flurbiprofen axetil and 0.5 mg/kg dezocine+1 μg/kg sufentanil for postoperative patient controlled intravenous analgesia(PCIA), respectively. The VAS score ≤3 points was maintained.If VAS score > 3 points, the 0.1 mg/kg dizocine was added in a single dose. The Ramsay sedation score and BCS comfort score among three groups were recorded after 2 h, 4 h, 8 h, 12 h and 24 h of surgery. The dose of dezocine, dose of additional dezocine, number of PCIA, postoperative bowel sounds recovery time, first anal exhaust time, first time out of bed activity, nausea, vomiting, bloating and dizziness among three groups were recorded. ResultsCompared with the DT group and DS group, the postoperative bowel sound recovery time, first anal exhaust time and first time of getting out of bed activity were significantly shortened(P < 0.05), and the postoperative nausea, abdominal distension and dizziness significantly reduced in the DF group(P < 0.05). There was no statistical significance in Ramsay sedation score and BCS comfort score among three groups after 2 h, 4 h, 8 h, 12 h and 24 h of surgery, and the differences of the dose of dezocine, dose of additional dezocine and number of PCIA were not statistically significant(P > 0.05). ConclusionsDezoxin combined with flurbiprofen axetil is more beneficial to the recovery of gastrointestinal function on the basis of satisfying postoperative analgesia.
2022, 47(3): 334-336.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.015
Abstract:
ObjectiveTo investigate the correlation between homocysteine(Hcy) level and MTHFR 677C/T gene polymorphism in patients with hypertension, and evaluate the effect of different doses of folic acid on different genotypes of hyperhomocysteinemia. MethodsThree hundred and fifty-nine patients with hypertension were screened for Hcy screening and MTHFR 677C/T gene detection. The gene type was divided into type CC, CT and TT, and 50 patients in each gene type were randomly divided into two groups(25 cases in each group), who were treated with 0.8 mg and 5 mg folic acid for 8 weeks to evaluate the therapeutic effect. ResultsAmong 359 hypertensive patients, 119 cases were combined with hyperhomocysteinemia, and the incidence rate was 55.43%. The incidence of hyperhomocysteinemia and plasma Hcy level in male patients were significantly higher than those in female patients(P < 0.01). The results of MTHFR 677C/T gene detection showed that among 359 patients, 161 cases were with type CC, 118 cases were with type CT, and 80 cases were with type TT. The incidence of hyperhomocysteinemia and plasma Hcy level in type TT hypertensive patients were higher than those in type CC and type CT patients(P < 0.01). There was no significant difference in plasma Hcy level between 0.8 mg group and 5 mg group of the same gene type before and after intervention(P > 0.05). The plasma Hcy level in 0.8 mg group and 5 mg group of different gene type was significantly lower than those before intervention(P < 0.01). The difference of Hcy level in different gene type in the same group after intervention was statistically significant(P < 0.01), the Hcy level of type CC in 0.8 mg group and 5 mg group was lower than that of type CT and TT(P < 0.05), and Hcy level of type CT in 0.8 mg group was lower than that of type TT(P < 0.05). ConclusionsThe Hcy level is correlated with MTHFR 677C/T gene polymorphism.Folic acid can effectively reduce hyperhomocysteinemia. There is no significant difference between high-dose folic acid and low-dose folic acid. The effect of folic acid intervention was different with different gene types.
2022, 47(3): 337-339.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.016
Abstract:
ObjectiveTo analyze the effects of Qingre Qushi decoction combined with warming acupuncture in the treatment of seborrheic alopecia. MethodsSeventy-two patients with seborrheic alopecia were randomly divided into the control group and observation group according to the random number table method(36 cases in each group). The control group was treated with routine drug, and the observation group was treated with Qingre Qushi decoction combined with warming acupuncture on the basis of routine drug treatment. The therapeutic effects between two groups were compared after 3 and 6 months of treatment. ResultsAfter 3 months of treatment, the total effective rate in observation group (80.56%) was higher than that in control group (50.00%) (P < 0.05). After 6 months of treatment, the total effective rate in observation group (94.44%) was higher than that in control group (63.89%) (P < 0.05). ConclusionsThe Qingre Qushi decoction combined with warming acupuncture in the treatment of seborrheic alopecia can effectively improve the clinical effects, it is worthy of clinical application.
2022, 47(3): 340-345.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.017
Abstract:
ObjectiveTo observe the correlation between the serum levels of CD64, interleukin-6(IL-6), platelet distribution width(PDW) and the distribution characteristics of pathogenic bacteria and infection prognosis in patients with acute myeloid leukemia complicated with infection. MethodsNinety patients with acute myeloid leukemia complicated with infection were selected as the study group, and 90 patients with acute myeloid leukemia without infection were selected as the control group. The serum levels of CD64, IL-6 and PDW in the two groups, the distribution characteristics of pathogenic bacteria in the study group, and the serum levels of the above indicators in patients with different infection type and infection prognosis were analyzed. The influencing factors of infection prognosis and death in patients with acute myeloid leukemia complicated with infection was explored, and the predictive value of the above serum indicators for the infection prognosis and death in patients with acute myeloid leukemia and infection was analyzed. ResultsThe serum levels of CD64, IL-6 and PDW in study group were higher than those in control group(P < 0.05). One hundred and eighteen strains of pathogenic bacteria were isolated from 90 patients in the study group, which were main Gram-negative bacteria, with a total of 78 strains(66.10%), followed by 30 strains(25.42%) of Gram-positive bacteria and 10 strains(8.47%) of fungi. In the study group, the serum levels of CD64, IL-6 and PDW in patients with Gram-negative bacteria infection were higher than those in patients with Gram-positive bacteria and fungi infection, the serum levels of the above indicators in patients with Gram-positive bacteria infection were higher than those in patients with fungi infection, and the serum levels of the above indicators in dead patients were higher than those in survival patients(P < 0.05). Logistic regression analysis showed that serum CD64, IL-6 and PDW were important risk factors for prognosis and death of patients with acute myeloid leukemia complicated with infection(P < 0.01). The ROC curve showed that the specificity and sensitivity of combination of serum CD64, IL-6 and PDW to predict the prognosis and death of patients with acute myeloid leukemia complicated with infection were 82.43% and 81.25%, respectively. ConclusionsThe serum levels of CD64, IL-6 and PDW in patients with acute myeloid leukemia complicated with infection are significantly increased, and the expressions are closely related to the distribution characteristics of pathogenic bacteria and infection prognosis.
2022, 47(3): 345-348.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.018
Abstract:
ObjectiveTo observe the curative effect of butylphthalide injection in the treatment of acute cerebral infarction, and to explore its influence on the levels of serum N-terminal pro-B-type natriuretic peptide(NT-proBNP) and homocysteine(Hcy). MethodsA total of 70 patients with acute cerebral infarction were randomly divided into observation group and control group, 35 cases in each group. The patients were given conventional treatment, and the observation group was additionally given butylphthalide sodium chloride injection 100 mL each time, intravenous drip, twice a day, continuous injection for 10-14 days. The clinical efficacies of two groups were compared, and the levels of serum NT-proBNP and Hcy were measured before and after treatment. The neurological deficits of the two groups were scored by National Institutes of Health stroke scale(NIHSS) before and after treatment, and the neurological status of the patients was evaluated by modified Rankin scale(mRS). ResultsThe total treatment efficiency in the observation group was 94.29%(33/35), which was significantly better than 71.43%(25/35) in the control group(P < 0.01). Before treatment, there was no significant difference in NIHSS score, mRS score and serum NT-proBNP and Hcy levels between the two groups(P > 0.05). After treatment, NIHSS score, mRS score, NT-proBNP and Hcy levels in the observation group were lower than those before treatment(P < 0.05), while NIHSS score, NT-proBNP and Hcy levels in the observation group were significantly lower than those in the control group(P < 0.01). ConclusionsButylphthalide injection can improve the neurological deficit of acute cerebral infarction and reduce the serum NT-proBNP and Hcy levels.
2022, 47(3): 349-352.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.019
Abstract:
ObjectiveTo investigate the effects of different doses of rosuvastatin on coronary atherosclerotic plaques and vascular endothelial cell function. MethodsEighty-six coronary atherosclerotic patients were randomly divided into the observation group and control gorup(43 cases in each gorup). The control group was treated with 10 mg of rosuvastatin, and the observation group was treated with 20 mg of rosuvastatin for 3 months. The volume of blood vessels, volume of lumen, volume of plaque, flow-mediated vasodilation(FMD), nitroglycerin-mediated vasodilation(NMD) and nitric oxide(NO) were compared between two groups, and the adverse reactions in two groups were also recorded. ResultsThere was no statistical significance in the vascular volume, lumen volume and plaque volume between two groups before treatment(P > 0.05). After treatment, the vascular volume, lumen volume and plaque volume in two groups decreased compared with those before treatment(P < 0.05), and which in observation group were less than those in control group(P < 0.05 to P < 0.01). There was no statistical significance in the FMD, NMD and NO between two groups before treatment(P > 0.05). After treatment, the FMD, NMD and NO in two groups increased compared with before treatment, and the FMD, NMD and NO in observation group were significantly higher than those in control group(P < 0.01). No acute cardiovascular events occurred in two groups during treatment. The incidence rate of adverse reactions in observation group and control group were 9.30%(4/43) and 6.98%(3/43), respectively, and the difference of which between two groups was not statistical significance(P > 0.05). ConclusionsCompared with 10 mg rosuvastatin, the 20 mg of rosuvastatin in the treatment of coronary atherosclerotic plaque can improve the plaque and vascular endothelial cell function, with good toleracnce and safety.
2022, 47(3): 353-355.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.020
Abstract:
ObjectiveTo investigate the effect of albumin and plasma volume expansion in the treatment of early onset preeclampsia. MethodsThe clinical data of 120 singleton pregnant women with early onset preeclampsia were analyzed retrospectively, and divided into plasma group(volume expanded with plasma, 40 cases), albumin group(volume expanded with albumin, 40 cases)and control group(no volume expanded, 40 cases). The expectant pregnant time, adverse perinatal outcome and incidence of maternal complications were compared among the three groups. ResultsThe gestational week of delivery in volume expansion group(albumin group and plasma group)was longer than that in control group(P < 0.05), and the incidence of fetal growth restriction was lower than those in control group(P < 0.05). The incidence of placental abruption and postpartum hemorrhage in plasma group was lower than that in control group(P < 0.05), and the incidence of renal function injury in plasma group was lower than that in albumin group(P < 0.05). ConclusionsThe efficacy of volume expansion therapy with albumin and plasma in the treatment of early onset preeclampsia is significant, which can prolong gestational week and improve perinatal outcome without increasing maternal complications.
2022, 47(3): 356-358.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.021
Abstract:
ObjectiveTo explore the effects of the growth hormone(GH) combined with antagonist regimen on pregnancy outcome in poor ovarian response(POR) patients treated with fertilization-embryo transfer(IVF-ET) in vitro. MethodsThe clinical data of 176 POR patients treated with IVF-ET were retrospectively analyzed, and the patients were divided into the GH group(GH+ group, 84 cases) and non-GH group(GH- group, 92 cases) according to whether GH was used or not. The egg number, MⅡ egg rate, normal fertilization rate, day 3 transferable embryo rate, day 3 high quality embryo rate, blastocyst formation rate, high quality blastocyst rate, clinical pregnancy rate and implantation rate were compared between two groups. ResultsThe egg number, daybryo rate, blastocyst formation rate, clinical pregnancy rate and implantation rate in GH+ group were higher than those in GH- group(P < 0.05 to P < 0.01).The differences of MⅡ egg rate, normal fertilization rate, high quality embryo rate on day 3 and high quality blastocyst rate between two groups were not statistically significant(P > 0.05). ConclusionsThe GH combined antagonist regimen is helpful for improving IVF-ET pregnancy outcomes in the POR patients.
2022, 47(3): 359-363.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.022
Abstract:
ObjectiveTo understand the status quo of social anxiety in nursing interns, to explore the relationship between psychological resilience, social anxiety and emotional intelligence, so as to provide reference for reducing social anxiety in nursing interns. MethodsA total of 235 nursing interns in a grade A tertiary hospital in Taizhou were investigated with general information questionnaire, interaction anxiousness scale, emotional intelligence scale and resilience scale. ResultsThe social anxiety level of nursing interns was(40.76±5.99) points, which was higher than that of the norm of Chinese college students(38.90±9.70) points(t=4.75, P < 0.05). The level of social anxiety of nursing interns in technical secondary school and junior college was significantly higher than that of undergraduate nursing interns(P < 0.01), and which in nursing interns with monthly living expenses of less than 600 yuan was higher than that of nursing interns with monthly living expenses of 600-1 000 yuan and more than 1 000 yuan(P < 0.05). The total scores of emotional intelligence and psychological resilience of nursing interns were(113.14±9.41) points and(87.52±8.53) points, respectively, which were at upper middle level.Emotional intelligence and psychological resilience were negatively correlated with social anxiety(P < 0.05 to P < 0.01). Psychological resilience partially mediated the relationship between social anxiety and emotional intelligence, and the mediating effect accounted for 35.6%. ConclusionsThe social anxiety level of nursing interns is higher. By improving the psychological resilience and emotional intelligence of nursing interns, the social anxiety level of nursing interns can be alleviated and the mental health of nursing interns can be maintained.
2022, 47(3): 364-368.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.023
Abstract:
ObjectiveTo investigate the personality characteristics of college students in excellent learning style classes, to analyze the personality differences between college students of different gender, origin and the only child or not, so as to find out the interactional effects of the above factors on personality factors of Cattell's sixteen personality factor questionnaire(16PF). MethodsA total of 1 360 college students in the excellent learning style classes in 2016, 2017 and 2018 were tested with 16PF. ResultsIn the excellent learning style classes, male students scored higher than female students on stability(C), suspicion(L), experimental(Q1) and automaticity(Q3)(P < 0.05 to P < 0.01), while scored lower than female students on sociability(A) and tension(Q4)(P < 0.01). Urban college students scored higher than rural students on bullying(E), excitability(F), courage(H), sensitivity(I), suspicion(L), fantasy(M) and sophistication(N)(P < 0.05 to P < 0.01), while scored lower than rural students on perseverance(G)(P < 0.05). The only child college students scored higher than non-only child students on I, L, M, N, Q1, and independence(Q2)(P < 0.05 to P < 0.01), while scored lower than non-only child students on perseverance(G)(P < 0.05). The interaction of gender and origin had a statistically significant effect on some personality factors included in 16PF of college students in excellent learning style classes(P < 0.05), and the effects of which on C, E, F, H, I, anxiety(O), Q3 and tension(Q4) were statistically significant(P < 0.05 to P < 0.01). ConclusionsThe 16PF personality characteristics of the students in the excellent learning style classes are different in three dimensions including gender, origin and the only child or not. The interaction of gender and origin has great influences on partial 16PF personality factors of the students in excellent learning style classes.
2022, 47(3): 368-372.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.024
Abstract:
ObjectiveTo explore the value of serum carbohydrate antigen 724(CA724) and human epididymis protein 4(HE4) in the diagnosis of pregnant women with ovarian cancer, and their correlation with ultrasound contrast parameters. MethodsSixty-eight pregnant women with ovarian cancer and 200 normal pregnant women were divided into the observation group and control group, respectively.The serum levels of CA724 and HE4 in two groups were detected, and the relationship between the above indicators and ovarian cancer in pregnant patients were analyzed. The correlation between CA724, HE4 and contrast-enhanced ultrasound parameters[time to peak(TTP) and enhanced intensity(EI)] after termination of pregnancy in the observation group with different pathological characteristics were analyzed. ResultsThe serum levels of CA724 and HE4 in observation were higher than those in control group(P < 0.01). The serum CA724 and HE4 were positively correlated with ovarian cancer in pregnancy(P < 0.05). The area under the curve(AUC) of the combined diagnosis of serum CA724 and HE4 in pregnancy with ovarian cancer was 0.940, and the diagnostic sensitivity and specificity of the combined detection of CA724 and HE4 were 88.24% and 92.00%, respectively. The differences of the levels of CA724, HE4, EI and TTP among the observation group with different FIGO stages, degree of differentiation and depth of invasion were statistically significant(P < 0.05 to P < 0.01). The serum CA724 and HE4 levels in observation group were positively correlated with EI, FIGO stage and invasion depth(P < 0.05 to P < 0.01), and negatively correlated with TTP and differentiation degree(P < 0.05 to P < 0.01). ConclusionsThe serum CA724 and HE4 levels increase significantly in pregnant patients with ovarian cancer, which can help the clinical diagnosis of pregnancy with ovarian cancer, and is closely related to the parameters of ultrasound contrast after pregnancy termination, ovarian cancer stage, depth of invasion and differentiation degree.
2022, 47(3): 373-376.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.025
Abstract:
ObjectiveTo investigate the drug resistance of carbapenem-resistant Acinetobacter baumannii(CRAB) in ICU of a hospital, and analyze the homology of the strains, so as to provide reference for the prevention and control measures of nosocomial infection. MethodsThirty CRAB strains isolated from ICU of a hospital from April to October 2017 were collected for drug sensitivity test, and their homology was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS). ResultsThe resistance rate of 30 CRAB strains to cefotetan, ceftriaxone, ceftazidime, cefepime and ciprofloxacin was 100.0%, and to levofloxacin was 73.3%.MALDI-TOF MS analysis showed that 30 CRAB strains were classified into three clusters, type Ⅰ(28 strains), type Ⅱ(1 strain) and type Ⅲ(1 strain), among which type Ⅰ was classified into type Ⅰa(26 strains), type Ⅰb(1 strain) and typeⅠc(1 strain), and 12 strains of type Ⅰa were isolated from August 2 to August 30 in 2017. ConclusionsIn April to October 2017, the main type of CRAB isolated from ICU of a hospital is type Ⅰa, and there is a trend of aggregation of type Ⅰa CRAB infection cases in August.
2022, 47(3): 376-380.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.026
Abstract:
ObjectiveTo analyze the implementation status of computer-based test in a medical college under the background of audit and evaluation, and to provide reference for the popularization and application of computer-based test. MethodsThe data related to the computer-based test carried out by a medical college from 2015 to 2019 were collected and analyzed.A self-designed questionnaire for teachers and students was used to conduct a random questionnaire survey, and the computer-based test cognition was analyzed. ResultsThe computer-based test rate of a medical college showed an increasing trend, with a computer-based test rate of 18%;teachers and students had a general understanding of computer-based test, teachers'willingness(78.02%) was high, and students'willingness(31.59%) was not high.The degree of understanding in teachers with management positions was higher than those with non-management positions, and the willingness in teachers who had lecturer title was higher than others(P < 0.05).There had the significant differences in students of different genders, grades, major and numbers of participating in computer-based test(P < 0.01), the degree of willingness in boys was higher than that in girls(P < 0.01), which in senior grade was higher than that in junior grade(P < 0.01), and was lower in engineering major(P < 0.01).There was still a gap between the requirements of computer hardware and software and computer-based test requirements. ConclusionsUnder the background of audit and evaluation, computer-based test in a medical college shows an increasing trend.It is necessary to carry out the targeted publicity and training, expand the influence and coverage, and optimize the allocation of hardware and software resources.
2022, 47(3): 381-385.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.027
Abstract:
ObjectiveTo construct the competence and quality evaluation index system in rural order-oriented free medical graduates, and provide a basis for the further reform and optimization of the training program for general outstanding talents in colleges and universities. MethodsDelphi expert consultation method was used to determine the evaluation index system of rural order-oriented free medical graduates' competence.The Yaahp hierarchy analysis software was used to calculate the weight of each index. ResultsThe evaluation index system of rural order-oriented free medical graduates' competence was constructed, which included 3 first-level indicators, 9 second-level indicators and 32 third-level indicators.The results of Delphi method showed that the positive coefficient of experts was 1, and the authority coefficient was 0.858.The results of index system weight showed that the weights of knowledge level, work skill and professional quality were 0.153 2, 0.420 7 and 0.426 1, respectively.Among the second-level indicators, the top three weight coefficients were professional ethics(0.261 4), community practice skills(0.152 6) and communication skills(0.130 8). ConclusionsThe competence and quality evaluation index system in rural order-oriented free medical graduates is successfully constructed, which can be used as a basis for assessing the competence of rural order-oriented free medical graduates.
2022, 47(3): 386-389.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.028
Abstract:
ObjectiveTo analyze the differentially expressed genes(DEGs)in osteosarcoma gene expression profiling chips using bioinformatics methods to identify the key genes involved in the development of osteosarcoma. MethodsThe expression profile gene chip data was downloaded from the GEO database, and the DEGs were screened using bioinformatics methods.The gene GO and KEGG pathway enrichment analysis of DEGs were performed on differentially expressed genes using the DAVID online database.The protein-protein interaction network analysis of DEGs of osteosarcoma was implemented using the STRING online software, MCODE plug-in of Cytoscape and cytoHubba plug-in to find the key gene. ResultsA total of 95 DEGs were screened, which were mainly enriched in cell adhesion molecules, antigen processing and presentation signaling pathways.The CD74, LAPTM5, CD163, MS4A6A, HLA-DRA, CD86, FCER1G, C1QB, TYROBP and AIF1 genes located at the core. ConclusionsCD74, LAPTM5, CD163, MS4A6A, HLA-DRA, CD86, FCER1G, C1QB, TYROBP and AIF1 may play an important role in the development of osteosarcoma.
2022, 47(3): 390-393.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.029
Abstract:
ObjectiveTo analyze the influence of National Natural Science Foundation of China(NSFC) funding on the output of high-level scientific research papers and academic influence indicators of provincial medical colleges, and provide the reference for relevant universities to further improve the output of high-level research papers and level of academic influence. MethodsThe A, B, C, D, E and F provincial medical colleges were selected as the research objects.With the help of the new generation InCites TM platform, A quantitative analysis was conducted on the high-level scientific research papers funded by NSFC from 2011 to 2020.The academic influence of high-level scientific research papers funded by NSFC were analyzed using the number of papers included in Web of Science, citation frequency, citation percentage, H index, citation influence, percentage of papers with top 1%(10%) citation times, highly cited papers and standardized citation influence of disciplines. ResultsThere was a significant gap in the number of high-level research papers funded by NSFC in the six sample universities, and the gap was consistent with the number of NSFC funded projects.The ranking of the number of papers in WOS was basically consistent with the ranking of the output of high-level research papers funded by NSFC.The total number of papers in top three universities funded by NSFC accounted for more than 50% of total number of papers included in WOS.The differences of the influence indexes of high-level research papers between funded by NSFC and non-NSFC were statistically significant among 6 sample universities(P < 0.01). ConclusionsNSFC funding can significantly improve the output and academic influence of high level scientific research papers in provincial medical colleges.
2022, 47(3): 394-397.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.030
Abstract:
ObjectiveTo investigate the current status of core competency among midwives in Anhui province and analyze its influencing factors, and to provide the reference for improvement of midwifery education. MethodsA total of 291 midwife in second and higher levels hospitals in Anhui province were investigated by the general self-efficacy scale, the midwife core competence questionnaire and the self-efficacy scale. ResultsThe mean score of the core competency among midwives was(170.40±29.13) points, which occupied in 77.5% of the total score; with higher scores in intrapartum care, post-partum care and public health care abilities, and lower scores in pre-gestational care, pregnancy care and neonatal care abilities.The single factor analysis showed that the working time, professional title, educational background, post of the last year and self-efficacy affected the core competency of midwives, the differences were statistically significant(P < 0.05 to P < 0.01).Multiple regression analysis showed that the working time, professional title and self-efficacy affected the improvement of core competency of midwives(P < 0.05). ConclusionsThe core competency of midwives in Anhui province is above the medium level, but there is still room for further improvement.In order to meet the increasing demand of pregnant and maternal women and their families' services, the educators should further improve Midwifery professional education and on-the-job training, and promote the overall improvement of the core competency among midwives.
2022, 47(3): 398-401.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.031
Abstract:
ObjectiveTo explore the narrative nursing combined with mindfulness based stress reduction intervention on Chinese perceived stress scale(CPSS) score, Memorial University of Newfoundland scale of happiness(MUNSH) score and quality of life in patients with chronic hepatitis B. MethodsNinety-eight patients with chronic hepatitis B were divided into the control group(n=49) and nursing group(n=49) according to the random number table method, and the control group and nursing group were intervened with conventional nursing and narrative nursing combined with mindfulness-based stress reduction intervention based on the conventional nursing, respectively.The CPSS score, MUNSH score and quality of life were compared between two groups. ResultsAfter nursing, the CPSS scores in two groups were lower than that before nursing(P < 0.01), and the CPSS score in nursing group was lower than that in control group(P < 0.01).After nursing, the each factor score and total score of MUNSH in nursing group were improved compared with those before nursing(P < 0.01), the negative factor score and total score in control group were improved compared with those before nursing(P < 0.01), and the each factor score and total score of MUNSH in nursing group were better than those in control group(P < 0.01).After nursing, the scores of WHO quality of life-BREF in two groups were higher than those before nursing(P < 0.05 to P < 0.01), and which in nursing group was higher than that in control group(P < 0.01). ConclusionsThe narrative nursing combined with mindfulness-based stress reduction intervention can significantly reduce the psychological pressure, improve the subjective happiness, and effectively improve the quality of life of patients with chronic hepatitis B.
2022, 47(3): 402-404.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.032
Abstract:
ObjectiveTo explore the application effect of full-time nurse's programmed management of sterile anesthesia consumable. MethodsThe management model of sterile anesthetic consumable preparing by anesthesiologist before operation were set as the control group(n=100), and the full-time nurse's programmed management of aseptic anesthesia consumable, in which the sterile anesthetic consumable was distributed 30 min before operation was set as the observation group(n=100).The preparation time of sterile consumable, satisfaction rate of anesthesiologist, on-the-job rate, qualified rate of consumable's valid period, and rate of missed payment of different anesthesia methods in the two groups were compared. ResultsThe preparation time and rate of missed payment in observation group were significantly lower than those in control group(P < 0.01).The satisfaction rate of anesthesiologist, on-the-job rate and qualified rate of consumable's valid period in observation group were significantly higher than those in control group(P < 0.01). ConclusionsThe full-time nurse's programmed management of sterile anesthesia consumable can improve the satisfaction rate and work efficiency of anesthesiologist, shorten the anesthesia preparation time, ensure the complete quantity and quality of sterile consumable, ensure the occurrence of no loss, no omission, no less recording and no expiration, and ensure the medical safety, which is worth popularizing and applying in clinic.
2022, 47(3): 405-408.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.033
Abstract:
ObjectiveTo explore the effects of the family empowerment nursing on patients with decompensated stage of liver cirrhosis and their families. MethodsEighty patients with decompensated stage of liver cirrhosis were randomly divided into the control group and empowerment group(40 case in each group).The control group was nursed with routine nursing method, and the empowerment group was additionally nursed with family empowerment nursing on the basis of routine nursing.The quality of life, psychological status, family load and comprehensive care ability in two groups were compared between before and after nursing. ResultsAfter intervention, the scores of Hamilton anxiety scale(HAMA) and Hamilton depression scale(HAMD) in empowerment group were significantly lower than those in control group(P < 0.01 and P < 0.05), and the dimensions score of quality of life in empowerment group were significantly higher than those in control group(P < 0.05 to P < 0.01).The scores of family care load in empowerment group were lower than those in control group(P < 0.05 to P < 0.01), and the score of comprehensive care ability in empowerment group was significantly higher than that in control group(P < 0.01). ConclusionsThe family empowerment nursing can significantly improve the psychological status and quality of life of patients with decompensated stage of liver cirrhosis, reduce the load of family members and improve their comprehensive care ability.
2022, 47(3): 409-413.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.034
Abstract:
ObjectiveTo analyze the influencing factors on sleep quality of shift nurses in operating room using three-factor scale model. MethodsA total of 513 operating room shift nurses were surveyed using the Pittsburgh sleep quality index(PSQI), and the data was analyzed using three-factor scale model(including sleep efficiency, sleep quality and daily life dysfunction). ResultsThe differences of PSQI among nurses with different labor relation, age and shift work experience were statistically significant(P < 0.05 to P < 0.01), while the differences of PSQI among nurses with only child, different marital status and children were not statistically significant(P>0.05).The results of multiple regression analysis showed that the shift experience was an independent influencing factor of sleep quality(P < 0.01).Among different shift work experience nurses, there was no statistical significance in the use of sleep medication and dimension of sleep disorder(P>0.05), and the differences of other dimensions and three factors were statistically significant(P < 0.05 to P < 0.01).The scores of PSQI factor 1, 2 and 3 in the shift nurses were higher than those in no shift experience nurses(P < 0.05), and the scores of PSQI factor 1 and 2 in the shift nurses within 6 months were also higher than those in no shift experience nurses(P < 0.05).The shift work experience was an independent influencing factor of sleep efficiency, sleep quality and dysfunction in daily life(P < 0.01). ConclusionsThe sleep quality of the operating room nurses with shift work experience within 6 months or shift work in progress is poor.The hospital should take appropriate intervention measures to improve the sleep quality of the operating room nurses.
2022, 47(3): 413-417.
doi: 10.13898/j.cnki.issn.1000-2200.2022.03.035
Abstract:
ObjectiveTo investigate the relationship among nurses' psychological capital, occupational well-being and job performance. MethodsA total of 283 registered nurses were selected as the study subjects from two grade A tertiary hospitals in Wuhan city using convenience sampling method.The subjects were surveyed with the questionnaires of the nurse psychological capital scale, occupational well-being scale and job performance inventory. ResultsThe total score of nurses' job performance was(202.22±16.95) points.There were no significant differences in the job performance of nurses with different gender and educational background(P>0.05), while differences in the job performance of nurses with different age, marital status, working year, professional title, department, and monthly income were statistically significant(P < 0.05 to P < 0.01).Pearson correlation analysis showed that the dimensions of nurses' task performance and situational performance were positively correlated with psychological capital, occupational well-being and other dimensions(P < 0.05 to P < 0.01), the anti-production performance was negatively correlated with the hope dimension in psychological capital(P < 0.05), but there was no obvious correlation between the dimensions of task performance and welfare(P>0.05).Multiple linear regression analysis showed that hope, resilience, job value and welfare benefit were the influencing factors of nurses' job performance(P < 0.01), which together explained 50.3% of the total variation. ConclusionsThe job performance of hospital nurses is at a medium level, which varies with age, marital status, working year, professional title, department, and monthly income.Hope, resilience, job value and welfare are the predictors of nurses'job performance.
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