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2009 Vol. 34, No. 8
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2009, 34(8): 653-656.
Abstract:
Objective: To establish a method of concentration and purification of erythrocytic stage Plasmodium vivax(P.v) by using combined WBC filtration and Percoll density centrifugation,and compare and analyze the immunoreactivity difference between infected RBC(iRBC) and normal RBC(nRBC) antigens prepared by saponin lysis and freeze-thawing by using pooled sera from P.v patients and normal controls.Methods: Leukocytes were removed by Plasmodipur filter from P.v patients' blood,iRBC was concentrated by 60% Percoll density gradient.Parasites were released by two methods:saponin lysis or freeze-thawing from iRBC.After sonication,P.v and nRBC soluble antigens which were prepared by the same method were compared by SDS-PAGE.Specific P.v antigen bands were defined by immunoblotting,pooled sera from P.v patients and normal controls were used as primary antibody to recognize P.v and nRBC antigen strip.Results: Ninety-nine of 115(86.1%) P.v infected blood samples filtrated by Plasmodipur filter to remove leukocytes and retained RBC reached an acceptable level(≤ 5/10 oil immersion field).Thirty-five WBC free samples were concentrated by 60% Percoll,and the iRBC percentage of 30 cases were raised over 60%.By SDS-PAGE analysis,there were 6 and 2 specific bands found in saponin lysis and freeze-thawing treatment P.v soluble antigens respectively.Immunoblotting analysis were showed that pooled P.v patients' sera can specifically recognized 22,24.5,29,35,36 kDa bands from saponin lysis treatment and 26,49,59,63,115,120 kDa bands from freeze-thawing treatment P.v soluble antigens.Combined using of Plasmodipur filter and 60% Percoll demonstrated of high efficiency in removing WBC and concentrating iRBC from P.v infected blood.Conclusions: There was an significant immunoreactivity difference between P.v soluble antigens prepared by saponin lysis and freeze-thawing treatment through using pooled P.v patients' sera.Twenty-two kDa P.v antigen prepared by saponin lysis treatment showed high immunoreactivity,it needs further study to evaluate its diagnostic usage.
Objective: To establish a method of concentration and purification of erythrocytic stage Plasmodium vivax(P.v) by using combined WBC filtration and Percoll density centrifugation,and compare and analyze the immunoreactivity difference between infected RBC(iRBC) and normal RBC(nRBC) antigens prepared by saponin lysis and freeze-thawing by using pooled sera from P.v patients and normal controls.Methods: Leukocytes were removed by Plasmodipur filter from P.v patients' blood,iRBC was concentrated by 60% Percoll density gradient.Parasites were released by two methods:saponin lysis or freeze-thawing from iRBC.After sonication,P.v and nRBC soluble antigens which were prepared by the same method were compared by SDS-PAGE.Specific P.v antigen bands were defined by immunoblotting,pooled sera from P.v patients and normal controls were used as primary antibody to recognize P.v and nRBC antigen strip.Results: Ninety-nine of 115(86.1%) P.v infected blood samples filtrated by Plasmodipur filter to remove leukocytes and retained RBC reached an acceptable level(≤ 5/10 oil immersion field).Thirty-five WBC free samples were concentrated by 60% Percoll,and the iRBC percentage of 30 cases were raised over 60%.By SDS-PAGE analysis,there were 6 and 2 specific bands found in saponin lysis and freeze-thawing treatment P.v soluble antigens respectively.Immunoblotting analysis were showed that pooled P.v patients' sera can specifically recognized 22,24.5,29,35,36 kDa bands from saponin lysis treatment and 26,49,59,63,115,120 kDa bands from freeze-thawing treatment P.v soluble antigens.Combined using of Plasmodipur filter and 60% Percoll demonstrated of high efficiency in removing WBC and concentrating iRBC from P.v infected blood.Conclusions: There was an significant immunoreactivity difference between P.v soluble antigens prepared by saponin lysis and freeze-thawing treatment through using pooled P.v patients' sera.Twenty-two kDa P.v antigen prepared by saponin lysis treatment showed high immunoreactivity,it needs further study to evaluate its diagnostic usage.
2009, 34(8): 656-658.
Abstract:
Objective: To explore the role and significance of abnormal expressions of phosphotase and tensin homolog deleted from chromsome 10(PTEN) and retinoblastoma(Rb) gene in the development of breast cancer.Methods: Immunohistochemical technique(S-P method) was used for detection of PTEN and Rb gene proteins in 150 cases of breast cancer and 30 cases of mammary benign proliferative lesions.The results were analyzed and compared with some clinicopathological parameters.Results: The protein expression levels of PTEN and Rb in breast cancer were significantly lower than those in benign proliferative lesions(P<0.005).Loss of expression of PTEN protein was obviously related to lymph nodes metastasis and the loss of expression of estrogen receptor(P<0.05).Loss of expression of Rb protein was not related to the clinicopathological parameters except the loss rate of expression was higher in grade Ⅲ.The positive association was found between the expressions of PTEN and Rb proteins(P<0.05).Conclusions: Expression rates of anti-oncogenes PTEN and Rb proteins are higher in benign proliferative lesions and decrease in breast cancer.The loss of inhibiting function of anti-oncogenes PTEN and Rb results in neoplastic transformation.Expression of PTEN relates to lymph nodes metastasis and the loss of expression of estrogen receptor,which shows a certein reference value to judge the prognosis of breast cancer.
Objective: To explore the role and significance of abnormal expressions of phosphotase and tensin homolog deleted from chromsome 10(PTEN) and retinoblastoma(Rb) gene in the development of breast cancer.Methods: Immunohistochemical technique(S-P method) was used for detection of PTEN and Rb gene proteins in 150 cases of breast cancer and 30 cases of mammary benign proliferative lesions.The results were analyzed and compared with some clinicopathological parameters.Results: The protein expression levels of PTEN and Rb in breast cancer were significantly lower than those in benign proliferative lesions(P<0.005).Loss of expression of PTEN protein was obviously related to lymph nodes metastasis and the loss of expression of estrogen receptor(P<0.05).Loss of expression of Rb protein was not related to the clinicopathological parameters except the loss rate of expression was higher in grade Ⅲ.The positive association was found between the expressions of PTEN and Rb proteins(P<0.05).Conclusions: Expression rates of anti-oncogenes PTEN and Rb proteins are higher in benign proliferative lesions and decrease in breast cancer.The loss of inhibiting function of anti-oncogenes PTEN and Rb results in neoplastic transformation.Expression of PTEN relates to lymph nodes metastasis and the loss of expression of estrogen receptor,which shows a certein reference value to judge the prognosis of breast cancer.
2009, 34(8): 659-662.
Abstract:
Objective: To investigate the expressions and clinical significance of human telomerase reverse transcriptase(hTERT) and survivin in gallbladder carcinoma.Methods: The expressions of hTERT and survivin in 60 cases of primary gallbladder carcinoma,15 cases of gallbladder adenoma and 15 cases of chronic cholecystitis were determined using streptavidin-peroxidase immunohistochemical method.Results: The positive rates of hTERT and survivin were significantly higher in gallbladder carcinoma group than in gallbladder adenoma group and chronic cholecystitis group(P<0.01).The positive expression of hTERT in gallbladder carcinoma had no significant correlation with the gender,age,histologic type,gallbladder stone,pathological grade,Nevin stage,metastasis or survival time after operation(P>0.05).The positive expression was significantly higher in Nevin staging Ⅲ-Ⅴ group than in Nevin staging Ⅰ-Ⅱ group(P<0.05).The positive expression of survivin was higher in lymph node or distant metastasis group than in non-metastasis group(P<0.05).In gallbladder carcinoma,the expression of hTERT had a positive correlation with that of survivin(P<0.05).Conclusions: Abnormal high expressions of hTERT and survivin protein are observed in gallbladder carcinoma,and there is a positive correlation between them.Both hTERT and survivin play an important role in the pathogenesis and progression of gallbladder carcinoma,and may have a cooperative effect on the carcinogenesis.High expression of hTERT is an early event in the pathogenesis of gallbladder carcinoma.The overexpression of survivin indicates malignant biological behavior of gallbladder carcinoma to some extent.
Objective: To investigate the expressions and clinical significance of human telomerase reverse transcriptase(hTERT) and survivin in gallbladder carcinoma.Methods: The expressions of hTERT and survivin in 60 cases of primary gallbladder carcinoma,15 cases of gallbladder adenoma and 15 cases of chronic cholecystitis were determined using streptavidin-peroxidase immunohistochemical method.Results: The positive rates of hTERT and survivin were significantly higher in gallbladder carcinoma group than in gallbladder adenoma group and chronic cholecystitis group(P<0.01).The positive expression of hTERT in gallbladder carcinoma had no significant correlation with the gender,age,histologic type,gallbladder stone,pathological grade,Nevin stage,metastasis or survival time after operation(P>0.05).The positive expression was significantly higher in Nevin staging Ⅲ-Ⅴ group than in Nevin staging Ⅰ-Ⅱ group(P<0.05).The positive expression of survivin was higher in lymph node or distant metastasis group than in non-metastasis group(P<0.05).In gallbladder carcinoma,the expression of hTERT had a positive correlation with that of survivin(P<0.05).Conclusions: Abnormal high expressions of hTERT and survivin protein are observed in gallbladder carcinoma,and there is a positive correlation between them.Both hTERT and survivin play an important role in the pathogenesis and progression of gallbladder carcinoma,and may have a cooperative effect on the carcinogenesis.High expression of hTERT is an early event in the pathogenesis of gallbladder carcinoma.The overexpression of survivin indicates malignant biological behavior of gallbladder carcinoma to some extent.
2009, 34(8): 662-665.
Abstract:
Objective: To investigate the mechanism of the effect of diethylstilbestrol on the proliferation and differentiation of hippocampal neural stem cells(NSCs) in fetal rats.Methods: Hippocampal neural stem cells from the embryo(gestational day 16) of fetal rats were prepared and divided into five groups:control,diethylstilbestrol 1×10-7 mol/L,diethylstilbestrol 1×10-8 mol/L,diethylstilbestrol 1×10-9 mol/L and BDNF 5×10-2 mg/L.The expressions of Nestin,neurone specific enolase(NSE) and glial fibrillary acidic protein(GFAP) were determined by immunohistochemistry,and the level of brain-derived neurotrophic factor(BDNF) mRNA was detected by RT-PCR.Results: Different doses of diethylstilbestrol could increase the area of neural spheres,quantity of NSE,and GFAP-positive cells.At a concentration of 10-8 mol/L,diethylstilbestrol facilitated the proliferation and differentiation of hippocampal NSCs of fetal rats the most,and the level of BDNA mRNA was the highest(P<0.01).Conclusions: Diethylstilbestrol enhances the proliferation of hippocampal NSCs in fetal rats,which is probably related to the up-regulation of the BDNF mRNAs' expression.
Objective: To investigate the mechanism of the effect of diethylstilbestrol on the proliferation and differentiation of hippocampal neural stem cells(NSCs) in fetal rats.Methods: Hippocampal neural stem cells from the embryo(gestational day 16) of fetal rats were prepared and divided into five groups:control,diethylstilbestrol 1×10-7 mol/L,diethylstilbestrol 1×10-8 mol/L,diethylstilbestrol 1×10-9 mol/L and BDNF 5×10-2 mg/L.The expressions of Nestin,neurone specific enolase(NSE) and glial fibrillary acidic protein(GFAP) were determined by immunohistochemistry,and the level of brain-derived neurotrophic factor(BDNF) mRNA was detected by RT-PCR.Results: Different doses of diethylstilbestrol could increase the area of neural spheres,quantity of NSE,and GFAP-positive cells.At a concentration of 10-8 mol/L,diethylstilbestrol facilitated the proliferation and differentiation of hippocampal NSCs of fetal rats the most,and the level of BDNA mRNA was the highest(P<0.01).Conclusions: Diethylstilbestrol enhances the proliferation of hippocampal NSCs in fetal rats,which is probably related to the up-regulation of the BDNF mRNAs' expression.
2009, 34(8): 666-668.
Abstract:
Objective: To study the expression of 5-lipoxygenase(5-LOX) in mucoepidermoid carcinoma(MEC),and to explore its probability of acting as a referential index in the diagnosis and treatment of mucoepidermoid carcinoma.Methods: The expressions of 5-LOX in 40 cases of MEC,20 cases of normal tissue of salivary glands and 20 cases of pleomorphic adenomas were detected by EliVisionTM plus immuniohistochemical method.All the data were analyzed statistically.Results: The positive staining rate of 5-LOX in mucoepidermoid carcinoma was 67.5%,which was significantly higher than that in pleomorphic adenoma(0.0%) and normal tissue of salivary glands(5.0%) (P<0.01).The positive staining rates of 5-LOX in high-differentiated,moderate-differentiated and low-differentiated cases of MEC were 50.0%,6/9 and 100.0%,respectively.The difference was significant(P<0.05).Conclusions: 5-LOX may be used as a biology referential index in diagnosing and differentiating the degree of mucoepidermoid carcinoma.
Objective: To study the expression of 5-lipoxygenase(5-LOX) in mucoepidermoid carcinoma(MEC),and to explore its probability of acting as a referential index in the diagnosis and treatment of mucoepidermoid carcinoma.Methods: The expressions of 5-LOX in 40 cases of MEC,20 cases of normal tissue of salivary glands and 20 cases of pleomorphic adenomas were detected by EliVisionTM plus immuniohistochemical method.All the data were analyzed statistically.Results: The positive staining rate of 5-LOX in mucoepidermoid carcinoma was 67.5%,which was significantly higher than that in pleomorphic adenoma(0.0%) and normal tissue of salivary glands(5.0%) (P<0.01).The positive staining rates of 5-LOX in high-differentiated,moderate-differentiated and low-differentiated cases of MEC were 50.0%,6/9 and 100.0%,respectively.The difference was significant(P<0.05).Conclusions: 5-LOX may be used as a biology referential index in diagnosing and differentiating the degree of mucoepidermoid carcinoma.
2009, 34(8): 669-671.
Abstract:
Objective: To observe the dynamic changes of vascular endothelial growth factor(VEGF) in the blood plasma of the newborns with hypoxic ischemic encephalopathy(HIE),and to investigate the correlation of VEGF with the pathogenesis and early diagnosis of HIE.Methods: Ninety full-term newborns with HIE were chosen as the experimental group,and the VEGF in plasma was examined on the 1st day,the 3rd day(acute stage) and the 7th day(the recovery stage) after birth using enzyme linked immunosorbent assay(ELISA);30 neonates with no nervous system diseases acted as control.Results: VEGF in the experimental group on the 1st day,3rd day and 7th day after birth increased significantly compared with that in the control(P<0.01),and it reached the peak on the 1st day,and then descended.The VEGF on the first day was much higher than that on the 7th day(P<0.01).Conclusions: VEGF can be used as a significant index in diagnosis of HIE.
Objective: To observe the dynamic changes of vascular endothelial growth factor(VEGF) in the blood plasma of the newborns with hypoxic ischemic encephalopathy(HIE),and to investigate the correlation of VEGF with the pathogenesis and early diagnosis of HIE.Methods: Ninety full-term newborns with HIE were chosen as the experimental group,and the VEGF in plasma was examined on the 1st day,the 3rd day(acute stage) and the 7th day(the recovery stage) after birth using enzyme linked immunosorbent assay(ELISA);30 neonates with no nervous system diseases acted as control.Results: VEGF in the experimental group on the 1st day,3rd day and 7th day after birth increased significantly compared with that in the control(P<0.01),and it reached the peak on the 1st day,and then descended.The VEGF on the first day was much higher than that on the 7th day(P<0.01).Conclusions: VEGF can be used as a significant index in diagnosis of HIE.
2009, 34(8): 671-674.
Abstract:
Objective: To explore the association of T-lymphocyte subsets and serum IL-12/sIL-2R levels with the clinicopathological factors in patients with gastric cancer.Methods: T-lymphocyte subsets and serum IL-12/sIL-2R levels in 80 patients with gastric cancer and 30 healthy controls were detected by immunofluorescence and enzyme linked immunosorbent assay(ELISA).Results: The CD3+ T cells,CD4+ T cells and CD4+/CD8+ ratio in the blood samples from the gastric cancer group were obviously lower than those from the control group(P<0.01);the IL-12 level in patients with gastric cancer was lower and the IL-2R level higher compared to that in the control(P<0.05).This changes were related to the tumor depth but not the histological types or the cell differentiation of the tumor.There was a significant correlation between serum IL-12/sIL-2R levels and CD4+/CD8+ ratio(P<0.05).Conclusions: The T-lymphocyte subsets and serum IL-12/sIL-2R levels can be used as an important immunology index of the genesis,development and metastasis of the gastric cancer.
Objective: To explore the association of T-lymphocyte subsets and serum IL-12/sIL-2R levels with the clinicopathological factors in patients with gastric cancer.Methods: T-lymphocyte subsets and serum IL-12/sIL-2R levels in 80 patients with gastric cancer and 30 healthy controls were detected by immunofluorescence and enzyme linked immunosorbent assay(ELISA).Results: The CD3+ T cells,CD4+ T cells and CD4+/CD8+ ratio in the blood samples from the gastric cancer group were obviously lower than those from the control group(P<0.01);the IL-12 level in patients with gastric cancer was lower and the IL-2R level higher compared to that in the control(P<0.05).This changes were related to the tumor depth but not the histological types or the cell differentiation of the tumor.There was a significant correlation between serum IL-12/sIL-2R levels and CD4+/CD8+ ratio(P<0.05).Conclusions: The T-lymphocyte subsets and serum IL-12/sIL-2R levels can be used as an important immunology index of the genesis,development and metastasis of the gastric cancer.
2009, 34(8): 675-677.
Abstract:
Objective: To study the therapeutic effect of large dose of intravenous immunoglobulin(IVIG) on central pontine myelinolysis(CPM).Methods: One case of CPM was treated with large dose of IVIG,and the related literature was reviewed.Results: Large dose of IVIG was effective on CPM.Conclusions: The therapy of large dose of IVIG can be used to treat CPM.
Objective: To study the therapeutic effect of large dose of intravenous immunoglobulin(IVIG) on central pontine myelinolysis(CPM).Methods: One case of CPM was treated with large dose of IVIG,and the related literature was reviewed.Results: Large dose of IVIG was effective on CPM.Conclusions: The therapy of large dose of IVIG can be used to treat CPM.
2009, 34(8): 677-679.
Abstract:
Objective: To study the therapeutic effect of Yiqihuoxue decoction on treatment of carotid arteriosclerosis plaque.Methods: Fifty-eight patients with carotid arteriosclerosis plaque were randomly divided into two groups,these cases in control group were given routine depressurization,decreasing the concentration of glucose and anti-platelet aggregation treatments,while Yiqihuoxue decoction was taken orally twice a day for these cases who belong to treatment group,200 ml every time.The carotid artery diameter,thickness of intima media,plaque size and the changes of haemorheology were recorded after 8 weeks.Results: Compared with pre-treatment,the changes of haemorheology were obviously improved in treatment group,intima media in carotid artery was obviously thinner.Plaque in carotid angiosclerosis was smaller or even disapeared.There was a statistical significance of efficiency variance in contrast to the control group(P<0.01).Conclusions: Yiqihuoxue decoction can play an effective role on treatment of carotid arteriosclerosis plaque.
Objective: To study the therapeutic effect of Yiqihuoxue decoction on treatment of carotid arteriosclerosis plaque.Methods: Fifty-eight patients with carotid arteriosclerosis plaque were randomly divided into two groups,these cases in control group were given routine depressurization,decreasing the concentration of glucose and anti-platelet aggregation treatments,while Yiqihuoxue decoction was taken orally twice a day for these cases who belong to treatment group,200 ml every time.The carotid artery diameter,thickness of intima media,plaque size and the changes of haemorheology were recorded after 8 weeks.Results: Compared with pre-treatment,the changes of haemorheology were obviously improved in treatment group,intima media in carotid artery was obviously thinner.Plaque in carotid angiosclerosis was smaller or even disapeared.There was a statistical significance of efficiency variance in contrast to the control group(P<0.01).Conclusions: Yiqihuoxue decoction can play an effective role on treatment of carotid arteriosclerosis plaque.
2009, 34(8): 680-681.
Abstract:
Objective: To assess the clinical effect of ultrasound-guided percutaneous injection of pingyangmycin on liver hemangioma.Methods: Sixty-eight cases of liver hemangioma underwent ultrasound-guided percutaneous injection of pingyangmycin.For tumors with a diameter <5 cm,5-8 cm and >8 cm,the dose was 8 mg,16 mg and 24-32 mg,respectively.All the cases were followed up for 6 months.Results: Forty-eight cases of liver hemangioma were cured by one operation,11 cases were cured by 2 treatments and 9 cases by 3.The blood in the hemangioma vanished,with an overall effective rate of 100%.After the operation,5 patients had low fever,8 had upper abdominal pain and dyspnea,and 2 patients felt sick and vomited.After treatment,all the symptoms disappeared.Conclusions: Ultrasound-guided percutaneous injection of pingyangmycin is an effective therapy for treatment for liver hemangioma.It is safe,convenient,easy to operate and has few complications
Objective: To assess the clinical effect of ultrasound-guided percutaneous injection of pingyangmycin on liver hemangioma.Methods: Sixty-eight cases of liver hemangioma underwent ultrasound-guided percutaneous injection of pingyangmycin.For tumors with a diameter <5 cm,5-8 cm and >8 cm,the dose was 8 mg,16 mg and 24-32 mg,respectively.All the cases were followed up for 6 months.Results: Forty-eight cases of liver hemangioma were cured by one operation,11 cases were cured by 2 treatments and 9 cases by 3.The blood in the hemangioma vanished,with an overall effective rate of 100%.After the operation,5 patients had low fever,8 had upper abdominal pain and dyspnea,and 2 patients felt sick and vomited.After treatment,all the symptoms disappeared.Conclusions: Ultrasound-guided percutaneous injection of pingyangmycin is an effective therapy for treatment for liver hemangioma.It is safe,convenient,easy to operate and has few complications
2009, 34(8): 682-684.
Abstract:
Objective: To evaluate the effect and side-effect of combined chemotherapy with paclitaxel(PTX) plus cisplatin(DDP) in the treatment of advanced non-small cell lung cancer(NSCLC).Methods: Thirty-four patients with advanced NSCLC received PTX 135 mg/m2 by intravenous infusion on d1,and DDP 75 mg/m2 by intravenous infusion on d2-4.The treatment was repeated every 21 days,up to 2 cycles.Results: Thirty-four patients were evaluable for efficacy,with an overall rate of 41.2%.The response rate of squamous cell carcinoma was no significant difference to that of adenocarcinoma.And there was also no significant difference between the Ⅲ stage patients and the Ⅳ stage patients(P>0.05).But the response rate of primary treatment was significantly different to that of relapse treatment(P<0.05).The main toxicities were myelosuppression,nausea and vomiting,and other side effects were mild.Conclusions: A high response rate can be obtained in advanced NSCLC by PTX plus DDP.PTX plus DDP combined therary is a promising antitumor protocol with tolerable toxicity.
Objective: To evaluate the effect and side-effect of combined chemotherapy with paclitaxel(PTX) plus cisplatin(DDP) in the treatment of advanced non-small cell lung cancer(NSCLC).Methods: Thirty-four patients with advanced NSCLC received PTX 135 mg/m2 by intravenous infusion on d1,and DDP 75 mg/m2 by intravenous infusion on d2-4.The treatment was repeated every 21 days,up to 2 cycles.Results: Thirty-four patients were evaluable for efficacy,with an overall rate of 41.2%.The response rate of squamous cell carcinoma was no significant difference to that of adenocarcinoma.And there was also no significant difference between the Ⅲ stage patients and the Ⅳ stage patients(P>0.05).But the response rate of primary treatment was significantly different to that of relapse treatment(P<0.05).The main toxicities were myelosuppression,nausea and vomiting,and other side effects were mild.Conclusions: A high response rate can be obtained in advanced NSCLC by PTX plus DDP.PTX plus DDP combined therary is a promising antitumor protocol with tolerable toxicity.
2009, 34(8): 684-685.
Abstract:
Objective: To evaluate the procedure of transurethral bipolar plasmakinetic enucleation for treatment of benign prostatic hyperplasia(BPH).Methods: A total of 40 patients with BPH were performed transurethral bipolar plasmakinetic enucleation of BPH.Results: The operation time ranged from 55 to 155 minutes(95 minutes on the average);perioperative blood loss was 80 to 600 ml(180 ml on the average);the average weight of resected prostate was 67.2 g.All the cases were followed up for 6 to 18 months.No transurethral resection syndrome or permanent urinary incontinence occurred.The maximum flow rate and international prostate symptoms score were significantly increased.Conclusions: Transurethral bipolar plasmakinetic enucleation is especially suitable for large prostate.It has many advantages such as thorough excision of the prostate,less blood loss in operation,less operation time and complication,higher security and quicker recovery.
Objective: To evaluate the procedure of transurethral bipolar plasmakinetic enucleation for treatment of benign prostatic hyperplasia(BPH).Methods: A total of 40 patients with BPH were performed transurethral bipolar plasmakinetic enucleation of BPH.Results: The operation time ranged from 55 to 155 minutes(95 minutes on the average);perioperative blood loss was 80 to 600 ml(180 ml on the average);the average weight of resected prostate was 67.2 g.All the cases were followed up for 6 to 18 months.No transurethral resection syndrome or permanent urinary incontinence occurred.The maximum flow rate and international prostate symptoms score were significantly increased.Conclusions: Transurethral bipolar plasmakinetic enucleation is especially suitable for large prostate.It has many advantages such as thorough excision of the prostate,less blood loss in operation,less operation time and complication,higher security and quicker recovery.
2009, 34(8): 686-687.
Abstract:
Objective: To explore the surgical indications of thyroid nodules and to summarize the experience in treating thyroid nodules.Methods: The clinical data and follow-up results of 112 patients with thyroid nodules who had received surgical treatment between Jan.2001 and Jun.2005 were analyzed retrospectively.Results: Temporary deadlimb was noted in 6 cases,cerchnus in 4 cases and 3 of them were temporary cerchnus,subcutaneous hemorrhage was observed in 1 patient and recurrence in 4 cases.Conclusions: Surgical indications of thyroid nodules should be identified before surgery.Reasonable surgical procedures should be chosen according to the condition of the patients,which may reduce the operation complications and the recurrence rate,and improve the prognosis of patients with thyroid nodules them.
Objective: To explore the surgical indications of thyroid nodules and to summarize the experience in treating thyroid nodules.Methods: The clinical data and follow-up results of 112 patients with thyroid nodules who had received surgical treatment between Jan.2001 and Jun.2005 were analyzed retrospectively.Results: Temporary deadlimb was noted in 6 cases,cerchnus in 4 cases and 3 of them were temporary cerchnus,subcutaneous hemorrhage was observed in 1 patient and recurrence in 4 cases.Conclusions: Surgical indications of thyroid nodules should be identified before surgery.Reasonable surgical procedures should be chosen according to the condition of the patients,which may reduce the operation complications and the recurrence rate,and improve the prognosis of patients with thyroid nodules them.
2009, 34(8): 688-691.
Abstract:
Objective: To analyze the advantages and disadvantages of percutaneous coronary intervention(PCI) via the radial artery or femoral artery.Methods: One hundred and fifty-seven cases undergoing coronary angiography(CAG) and/or PCI were divided into two groups:radial approach group(98 cases) and femoral approach group(59 cases).The rates of success and complication were observed and compared statistically.Results: The success rate of CAG in the radial artery group and femoral artery group was 95.92% and 98.31%,respectively;and the success rate of PCI in the radial approach group and the femoral approach group was 94.87% and 96.15%,respectively(P>0.05);the incidence of local complication in the radial approach group was significantly lower than that in the femoral approach group(5.1% vs 27.1% and 7.7% vs 46.2%) (P<0.01).Conclusions: PCI via the radial artery or the femoral artery has a similar success rate,but PCI via the radial artery has a lower incidence of local complication.
Objective: To analyze the advantages and disadvantages of percutaneous coronary intervention(PCI) via the radial artery or femoral artery.Methods: One hundred and fifty-seven cases undergoing coronary angiography(CAG) and/or PCI were divided into two groups:radial approach group(98 cases) and femoral approach group(59 cases).The rates of success and complication were observed and compared statistically.Results: The success rate of CAG in the radial artery group and femoral artery group was 95.92% and 98.31%,respectively;and the success rate of PCI in the radial approach group and the femoral approach group was 94.87% and 96.15%,respectively(P>0.05);the incidence of local complication in the radial approach group was significantly lower than that in the femoral approach group(5.1% vs 27.1% and 7.7% vs 46.2%) (P<0.01).Conclusions: PCI via the radial artery or the femoral artery has a similar success rate,but PCI via the radial artery has a lower incidence of local complication.
2009, 34(8): 691-692.
Abstract:
Objective: To explore the clinical significance of D-dimer(DDR) and C reative protein(CRP) in patients with chronic pulmonary heart disease.Methods: The levels of DDR and CRP were measured in the plasma of 41 patients with chronic pulmonary heart disease and 30 healthy controls.Results: The plasma levels of DDR and CRP were significantly different between the patients with chronic pulmonary heart disease and the healthy controls(P<0.01).Conclusions: The increase of serum CRP indicates pathophysiology of pneumonia.Serum DDR reveals the patients' hypercoagulabale state and is of helps to the diagnosis of microthromb in the lung.
Objective: To explore the clinical significance of D-dimer(DDR) and C reative protein(CRP) in patients with chronic pulmonary heart disease.Methods: The levels of DDR and CRP were measured in the plasma of 41 patients with chronic pulmonary heart disease and 30 healthy controls.Results: The plasma levels of DDR and CRP were significantly different between the patients with chronic pulmonary heart disease and the healthy controls(P<0.01).Conclusions: The increase of serum CRP indicates pathophysiology of pneumonia.Serum DDR reveals the patients' hypercoagulabale state and is of helps to the diagnosis of microthromb in the lung.
2009, 34(8): 693-695.
Abstract:
Objective: To evaluate the improved total vaginal hysterectomy(TVH),and to explore its application in basic level hospitals by comparing TVH and total abdominal hysterectomy(TAH) for patients with non-prolapse uterus and a history of pelvic surgery.Methods: Improved TVH was conducted to 26 cases of large non-prolapsed uterus and with a history of pelvic surgery,and the result was compared with that of the cases receiving TAH at the same period.Results: The time of anal discharge,postoperative fever and hospital stay were significantly shorter in the TVH group than those in the TAH group(P<0.01),but there was no significant difference in operation time or bleeding amount between the two groups(P>0.05).Conclusions: The recovery is faster in the cases receiving improved TVH than in those receiving TAH.The improved TVH is also safe to the patients with a history of pelvic surgery and big uterus.It can be popularized in basic level hospitals.
Objective: To evaluate the improved total vaginal hysterectomy(TVH),and to explore its application in basic level hospitals by comparing TVH and total abdominal hysterectomy(TAH) for patients with non-prolapse uterus and a history of pelvic surgery.Methods: Improved TVH was conducted to 26 cases of large non-prolapsed uterus and with a history of pelvic surgery,and the result was compared with that of the cases receiving TAH at the same period.Results: The time of anal discharge,postoperative fever and hospital stay were significantly shorter in the TVH group than those in the TAH group(P<0.01),but there was no significant difference in operation time or bleeding amount between the two groups(P>0.05).Conclusions: The recovery is faster in the cases receiving improved TVH than in those receiving TAH.The improved TVH is also safe to the patients with a history of pelvic surgery and big uterus.It can be popularized in basic level hospitals.
2009, 34(8): 695-696.
Abstract:
Objective: To investigate the role of serum total bile acid in discriminating complicated hemorrhage of liver cirrhosis and peptic ulcer.Methods: The clinical data of 71 patients with complicated hemorrhage of liver cirrhosis or peptic ulcer were analyzed retrospectively,and 40 normal people acted as control subjects.Results: The mean serum total bile acid of the patients with decompensated cirrhosis complicated with hemorrhage(30.75±28.70) μmol/L was significantly higher than that of the patients with peptic ulcer complicated with hemorrhage(4.80±3.87) μmol/L and the control(4.50±3.60) μmol/L(P<0.01).Conclusions: It is simple and effective to discriminate liver cirrhosis and peptic ulcer complicated with hemorrhage by serum total bile acid.
Objective: To investigate the role of serum total bile acid in discriminating complicated hemorrhage of liver cirrhosis and peptic ulcer.Methods: The clinical data of 71 patients with complicated hemorrhage of liver cirrhosis or peptic ulcer were analyzed retrospectively,and 40 normal people acted as control subjects.Results: The mean serum total bile acid of the patients with decompensated cirrhosis complicated with hemorrhage(30.75±28.70) μmol/L was significantly higher than that of the patients with peptic ulcer complicated with hemorrhage(4.80±3.87) μmol/L and the control(4.50±3.60) μmol/L(P<0.01).Conclusions: It is simple and effective to discriminate liver cirrhosis and peptic ulcer complicated with hemorrhage by serum total bile acid.
2009, 34(8): 697-698.
Abstract:
Objective: To evaluate the effect of sufentanil in combination with propofol as general anesthesia induction on the hemodynamic response during tracheal intubation period.Methods: Forty patients with ASA Ⅰ-Ⅱ stages laparoscopic cholecystectomy were randomized into 2 groups,sufentanil group and fentanyl group,20 cases of each group.Anesthesia induction were done through intravenous injection with midazolam at 0.05 mg/kg,propofol at 1-1.5 mg/kg,sufentanil at 0.5 μg/kg or fentanyl at 3-5 μg/kg,vecuronium at 0.1 mg/kg.Five minutes later,tracheal intubation was done.The hemodynamics before induction,during induction and 5 min after intubation were recorded.Results: In fentanyl group,in contrast to before induction,mean arterial pressure(MAP) and heart rate(HR) had more significant differences after anesthesia induction(P<0.01).In Sufentanil group,these parameters had no significant difference(P>0.05).Between the two groups within 5 min after intubation,MAP and HR had significant differences(P<0.01).Conclusions: Sufentanil in combination with propofol as general anesthesia induction is more stable and less stress response on blood hemodynamics.
Objective: To evaluate the effect of sufentanil in combination with propofol as general anesthesia induction on the hemodynamic response during tracheal intubation period.Methods: Forty patients with ASA Ⅰ-Ⅱ stages laparoscopic cholecystectomy were randomized into 2 groups,sufentanil group and fentanyl group,20 cases of each group.Anesthesia induction were done through intravenous injection with midazolam at 0.05 mg/kg,propofol at 1-1.5 mg/kg,sufentanil at 0.5 μg/kg or fentanyl at 3-5 μg/kg,vecuronium at 0.1 mg/kg.Five minutes later,tracheal intubation was done.The hemodynamics before induction,during induction and 5 min after intubation were recorded.Results: In fentanyl group,in contrast to before induction,mean arterial pressure(MAP) and heart rate(HR) had more significant differences after anesthesia induction(P<0.01).In Sufentanil group,these parameters had no significant difference(P>0.05).Between the two groups within 5 min after intubation,MAP and HR had significant differences(P<0.01).Conclusions: Sufentanil in combination with propofol as general anesthesia induction is more stable and less stress response on blood hemodynamics.
2009, 34(8): 699-701.
Abstract:
Objective: The study was to evaluate the efficacy and tolerability of docetaxel plus cisplatin in the treatment of non-small cell lung cancer and breast cancer.Methods: Seventy-five patients were received docetaxel 70 mg/m2 d1 plus cisplatin 40 mg d2-4,and the plan was repeated every 3 weeks.All the patients had to received at least 2 cycles of chemotherapy.Results: The response rate of the study(CR+PR) was 29.3%(non-small lung cancer was 27.9%,breast cancer was 31.3%).The main toxicities were myelo suppression,alimentary canal reactions,muscle soreness and fever,and the rate of grade Ⅳ was low.Conclusions: Docetaxel plus cisplatin can work effectively and safely in the treatment of advanced non-small lung cancer and breast cancer,and the toxicity can be toleranced.
Objective: The study was to evaluate the efficacy and tolerability of docetaxel plus cisplatin in the treatment of non-small cell lung cancer and breast cancer.Methods: Seventy-five patients were received docetaxel 70 mg/m2 d1 plus cisplatin 40 mg d2-4,and the plan was repeated every 3 weeks.All the patients had to received at least 2 cycles of chemotherapy.Results: The response rate of the study(CR+PR) was 29.3%(non-small lung cancer was 27.9%,breast cancer was 31.3%).The main toxicities were myelo suppression,alimentary canal reactions,muscle soreness and fever,and the rate of grade Ⅳ was low.Conclusions: Docetaxel plus cisplatin can work effectively and safely in the treatment of advanced non-small lung cancer and breast cancer,and the toxicity can be toleranced.
2009, 34(8): 701-703.
Abstract:
Objective: To compare the clinical results of modified laparoscopy-assisted vaginal hysterectomy(LAVH) and through vaginal hysterectomy(TVH) in treatment of big myoma(≥ 12 gestation weeks).Methods: The clinical data of cases with big myoma who had undergone uterus hysterectomy in 2008 in the First People's Hospital of Shanghai were collected.Thirty-six cases were treated by LAVH and 36 cases by TVH.The operation time,blood loss in the operation,body temperature and passage of gas by anus after the operation were analyzed and compared between the two groups.Results: The operation time and bleeding in the operation were decreased obviously in the modified LAVH group compared with those in the TVH group(P<0.01).But the body temperature and passage of gas by anus after operation were not statistically different between the two groups(P>0.05).In TVH group,rectum injury was observed in 1 case,which was mended by cutting the abdomen operation,and vaginal stump bleeding was noted in 2 cases,which was managed after reexamination through the vagina.The operation was all successful in the modified LAVH group and no complication occurred.Conclusions: Reformed LAVH makes the operation less difficult and shortens the operation time,which promises a wide prospect in the basal hospitals.
Objective: To compare the clinical results of modified laparoscopy-assisted vaginal hysterectomy(LAVH) and through vaginal hysterectomy(TVH) in treatment of big myoma(≥ 12 gestation weeks).Methods: The clinical data of cases with big myoma who had undergone uterus hysterectomy in 2008 in the First People's Hospital of Shanghai were collected.Thirty-six cases were treated by LAVH and 36 cases by TVH.The operation time,blood loss in the operation,body temperature and passage of gas by anus after the operation were analyzed and compared between the two groups.Results: The operation time and bleeding in the operation were decreased obviously in the modified LAVH group compared with those in the TVH group(P<0.01).But the body temperature and passage of gas by anus after operation were not statistically different between the two groups(P>0.05).In TVH group,rectum injury was observed in 1 case,which was mended by cutting the abdomen operation,and vaginal stump bleeding was noted in 2 cases,which was managed after reexamination through the vagina.The operation was all successful in the modified LAVH group and no complication occurred.Conclusions: Reformed LAVH makes the operation less difficult and shortens the operation time,which promises a wide prospect in the basal hospitals.
2009, 34(8): 704-705.
Abstract:
Objective: To investigate the efficacy and safety of the chemotherapy of paclitaxel(PTX) combined with epirubicin(EPI) in treatment of patients with advanced breast cancer.Methods: Twenty-two patients with advanced breast cancer were treated with the combination chemotherapy of PTX and EPI.PTX 150 mg/m2 and EPI 75 mg/m2 were administered on day 1 every three weeks.The efficacy and toxicity were evaluated after two cycles.Results: Among the 22 cases,complete response(CR) was observed in 2 cases(9.1%),partial response(PR) in 12(54.5%),stable disease(SD) in 7(31.8%) and progressive disease(PD) in 1(4.5%).The overall response rate was 63.6%(14/22).The main side effects were myelosuppression,alopecia and vomiting.The overall response rates in the initially treated group and re-treated group were 6/9 and 61.5%,respectively.The difference was not statistically significant(P>0.05).Conclusions: The combination therapy of paclitaxel and epirubicin is safe and effective in treatment of patients with advanced breast cancer,and the toxicity is tolerable.
Objective: To investigate the efficacy and safety of the chemotherapy of paclitaxel(PTX) combined with epirubicin(EPI) in treatment of patients with advanced breast cancer.Methods: Twenty-two patients with advanced breast cancer were treated with the combination chemotherapy of PTX and EPI.PTX 150 mg/m2 and EPI 75 mg/m2 were administered on day 1 every three weeks.The efficacy and toxicity were evaluated after two cycles.Results: Among the 22 cases,complete response(CR) was observed in 2 cases(9.1%),partial response(PR) in 12(54.5%),stable disease(SD) in 7(31.8%) and progressive disease(PD) in 1(4.5%).The overall response rate was 63.6%(14/22).The main side effects were myelosuppression,alopecia and vomiting.The overall response rates in the initially treated group and re-treated group were 6/9 and 61.5%,respectively.The difference was not statistically significant(P>0.05).Conclusions: The combination therapy of paclitaxel and epirubicin is safe and effective in treatment of patients with advanced breast cancer,and the toxicity is tolerable.
2009, 34(8): 706-707.
Abstract:
Objective: To investigate the managements of cystic artery bleeding during laparoscopic cholecystectomy(LC).Methods: Sixteen cases with cystic artery bleeding following LC were analyzed retrospectively.These methods including absorbing tractional pore completely,exchanging the clips,clipping the artery using titanium clips were utilized to against bleeding.Results: No rebleeding or biliary injury was happened after operation.Only one patient was transformed to open surgery during operation.Conclusions: Cystic artery bleeding is a common cause in the bleeding of laparoscopic cholecystectomy.For a surgeon,mastering proficient laparoscopic technique and management can obtain satisfactory results.
Objective: To investigate the managements of cystic artery bleeding during laparoscopic cholecystectomy(LC).Methods: Sixteen cases with cystic artery bleeding following LC were analyzed retrospectively.These methods including absorbing tractional pore completely,exchanging the clips,clipping the artery using titanium clips were utilized to against bleeding.Results: No rebleeding or biliary injury was happened after operation.Only one patient was transformed to open surgery during operation.Conclusions: Cystic artery bleeding is a common cause in the bleeding of laparoscopic cholecystectomy.For a surgeon,mastering proficient laparoscopic technique and management can obtain satisfactory results.
2009, 34(8): 707-708.
Abstract:
Objective: To explore an effective method for radical correction of axillary osmidrosis.Methods: Two or three incisions with a length of 3 cm were excided in the skin fold of axillary,and the flap was bluntly dissected 2 mm under the dermis;all the apocrine glands,sweat glands and hair-follicle of the flap were pruned to a full or split thickness.After the flap was irrigated and the hemorrhage arrested,the incision was sutured and dressed with pressure.Results: The peculiar smells were eliminated in all the 34 cases;the flaps were all alive and no visible scar was left in the field of operation.Conclusions: Miniature cut and pruning tissues under dermis is an effective method for radical correction of osmidrosis.
Objective: To explore an effective method for radical correction of axillary osmidrosis.Methods: Two or three incisions with a length of 3 cm were excided in the skin fold of axillary,and the flap was bluntly dissected 2 mm under the dermis;all the apocrine glands,sweat glands and hair-follicle of the flap were pruned to a full or split thickness.After the flap was irrigated and the hemorrhage arrested,the incision was sutured and dressed with pressure.Results: The peculiar smells were eliminated in all the 34 cases;the flaps were all alive and no visible scar was left in the field of operation.Conclusions: Miniature cut and pruning tissues under dermis is an effective method for radical correction of osmidrosis.
2009, 34(8): 709-710.
Abstract:
Objective: To elucidate the method and result of transurethral holmium laser therapy for treatment of cystitis glandularis(CG).Methods: Twenty-seven cases of CG confirmed pathologically using cystoscopy were treated by the transurethral holmium laser and received postoperative chemotherapy of pirarubicin bladder irrigation.Results: The operation was successful in all the 27 cases,and the follow-up lasted for 8 to 27 months.Nineteen cases were cured,6 alleviated,and 2 relapsed;no carcinomatous change was observed.Conclusions: The transurethral holmium laser therapy is an effective,convenient and safe technique with a low recurrence rate for treatment of CG.It is worthy of popularization in clinic.
Objective: To elucidate the method and result of transurethral holmium laser therapy for treatment of cystitis glandularis(CG).Methods: Twenty-seven cases of CG confirmed pathologically using cystoscopy were treated by the transurethral holmium laser and received postoperative chemotherapy of pirarubicin bladder irrigation.Results: The operation was successful in all the 27 cases,and the follow-up lasted for 8 to 27 months.Nineteen cases were cured,6 alleviated,and 2 relapsed;no carcinomatous change was observed.Conclusions: The transurethral holmium laser therapy is an effective,convenient and safe technique with a low recurrence rate for treatment of CG.It is worthy of popularization in clinic.
2009, 34(8): 711-714.
Abstract:
Objective: To determine the effect and side effect of ritodrine in the premature labor and investigate the clinical significance.Methods: One hundred cases of premature labor were randomly divided into observation group and control group.Observation group consisted of 60 cases were given with ritodrine at 0.05 mg/min concentration firstly,and the concentration was added gradually until the uterine contraction was vanished and the medication was continued for 12-18 hours,then the medicine taken orally last for 7 days.Control group consisted of 40 cases were given with magnesium sulfate intravenously,the first 4 g was given within 30 minutes,then 1-2 g per hour was given until the uterine contraction was vanished.The success rate of fetus protection,the time of uterine contraction vanished,the incidence of hypopotassaemia,the changes of heart rate,the effect of prolong gestational age,the rate of postpartum hemorrhage rate,neonatal weight and the Apgar score were compared between the two groups.Results: Percent of failure cases is 11.7% in observation group and 12.5% in control group;prolong gestational age is(1.00±0.7) week in observation group and(0.87±0.6) week in control group,which was shown no significant difference between two groups(P>0.05).The time of uterine contraction vanished in observation group was shorter than in control group(P<0.05).The incidence of hypopotassaemia was higher in observation group(P<0.01).The rate of postpartum hemorrhage,neonatal weight and the Apgar score were no significant difference.Conclusions: Ritodrine can inhibit uterine contraction of pregnant woman which prevent the occurance of premature labor effectively,but the incidence of hypopotassaemia and other side effects will be cautioned.
Objective: To determine the effect and side effect of ritodrine in the premature labor and investigate the clinical significance.Methods: One hundred cases of premature labor were randomly divided into observation group and control group.Observation group consisted of 60 cases were given with ritodrine at 0.05 mg/min concentration firstly,and the concentration was added gradually until the uterine contraction was vanished and the medication was continued for 12-18 hours,then the medicine taken orally last for 7 days.Control group consisted of 40 cases were given with magnesium sulfate intravenously,the first 4 g was given within 30 minutes,then 1-2 g per hour was given until the uterine contraction was vanished.The success rate of fetus protection,the time of uterine contraction vanished,the incidence of hypopotassaemia,the changes of heart rate,the effect of prolong gestational age,the rate of postpartum hemorrhage rate,neonatal weight and the Apgar score were compared between the two groups.Results: Percent of failure cases is 11.7% in observation group and 12.5% in control group;prolong gestational age is(1.00±0.7) week in observation group and(0.87±0.6) week in control group,which was shown no significant difference between two groups(P>0.05).The time of uterine contraction vanished in observation group was shorter than in control group(P<0.05).The incidence of hypopotassaemia was higher in observation group(P<0.01).The rate of postpartum hemorrhage,neonatal weight and the Apgar score were no significant difference.Conclusions: Ritodrine can inhibit uterine contraction of pregnant woman which prevent the occurance of premature labor effectively,but the incidence of hypopotassaemia and other side effects will be cautioned.
2009, 34(8): 721-723.
Abstract:
Objective: To investigate the relationship between the value of cerebral blood volume(CBV) and Ras homologue A(RhoA) expression.Methods: CT perfusion imaging was performed in thirty-one cases of astrocytomas(14 cases of low grade astrocytomas and 17 cases of high grade astrocytomas).The CBV and RhoA expression were determined by immunohistochemical method,and the relation between them was analyzed.Results: The CBV value and immunoeactivity score in high grade astrocytoma tumors were higher than that in low grade astrocytoma tumors(P<0.01).There was a positive correlation between CBV value and RhoA expression(P<0.01).Conclusions: CT perfusion imaging is valuable in preoperative grading of astrocytoma,and CBV is advantageous in evaluating the invasion of astrocytomas.
Objective: To investigate the relationship between the value of cerebral blood volume(CBV) and Ras homologue A(RhoA) expression.Methods: CT perfusion imaging was performed in thirty-one cases of astrocytomas(14 cases of low grade astrocytomas and 17 cases of high grade astrocytomas).The CBV and RhoA expression were determined by immunohistochemical method,and the relation between them was analyzed.Results: The CBV value and immunoeactivity score in high grade astrocytoma tumors were higher than that in low grade astrocytoma tumors(P<0.01).There was a positive correlation between CBV value and RhoA expression(P<0.01).Conclusions: CT perfusion imaging is valuable in preoperative grading of astrocytoma,and CBV is advantageous in evaluating the invasion of astrocytomas.
2009, 34(8): 726-728.
Abstract:
Objective: To investigate the surveillance data of bacterial resistance in Lu'an city hospitals in 2008.Methods: Bacterial susceptibility experiments were performed on 102 samples clinically collected from 3 hospitals in Lu'an city,using routine custom dilution MIC method according to the CLSI(2008) guidelines;the resistance,intermediate and susceptible rates of the bacteria to antimicrobial agents were measured.Results: Among the 102 bacteria samples,Escherichia coli accounted for 27,Acinetobacter baumannii 25,Pseudomonas aeruginosa 23,Klebsiella 18,Stenotrophomonas maltophilia 7 and others 2.Carbapenems were the most effective antimicrobial agents against Gram-negative bacilli,and other effective ones were piperacillin-tazobactam(73.5%),amikacin(67.6%) and fosfomycin(62.7%).Gram-negative bacilli showed high resistance to ampicillin(96.1%),cefoxitin(83.3%) and aztreonam(81.4%),and were also noticeably resistant to cefoperazone/sulbactam(51.0%) and cefepime(39.4%).Conclusions: We should administer antimicrobial drugs according to the "Guiding principles for the clinical use of antibacterial drugs",and rationally choose antimicrobial agents in treatment of bacterial infections to control the bacterial resistance rates.
Objective: To investigate the surveillance data of bacterial resistance in Lu'an city hospitals in 2008.Methods: Bacterial susceptibility experiments were performed on 102 samples clinically collected from 3 hospitals in Lu'an city,using routine custom dilution MIC method according to the CLSI(2008) guidelines;the resistance,intermediate and susceptible rates of the bacteria to antimicrobial agents were measured.Results: Among the 102 bacteria samples,Escherichia coli accounted for 27,Acinetobacter baumannii 25,Pseudomonas aeruginosa 23,Klebsiella 18,Stenotrophomonas maltophilia 7 and others 2.Carbapenems were the most effective antimicrobial agents against Gram-negative bacilli,and other effective ones were piperacillin-tazobactam(73.5%),amikacin(67.6%) and fosfomycin(62.7%).Gram-negative bacilli showed high resistance to ampicillin(96.1%),cefoxitin(83.3%) and aztreonam(81.4%),and were also noticeably resistant to cefoperazone/sulbactam(51.0%) and cefepime(39.4%).Conclusions: We should administer antimicrobial drugs according to the "Guiding principles for the clinical use of antibacterial drugs",and rationally choose antimicrobial agents in treatment of bacterial infections to control the bacterial resistance rates.
2009, 34(8): 729-731.
Abstract:
Objective: To investigate the changes and the clinical value of biochemical markers of myocardial damage in patients undergoing operation of heart valve replacement.Methods: The creatine kinase(CK),creatine kinase-MB(CK-MB),cardiac treponin I(cTnI),C reactive protein(CRP),lactate dehydrogenase(LDH) and hydroxybutyrate dehydrogenase(HBDH) in 40 patients receiving heart valve replacement were measured before operation,instantly after extracorporeal circulation,and at 6,24,48,72 hour and 6 days after the operation,and the changes were analyzed.Results: All the biochemical markers of myocardial damage were within normal limits except CRP before operation.The figures of cTnI and CK-MB increased significantly at 6 hour after operation(P<0.01).Aspartate aminotrans ferase(AST),LDH and HBDH rose significantly at 24 hour after operation.CRP reached the peak at 48 hour after operation.The two variables of cTnI and CK-MB in serum showed a positive linear correlation and had a higher correlativity comparable to other markers.Conclusions: All the biochemical markers of myocardial damage present abnormal changes successively during the perioperation of cardiac valve replacement.Monitoring the myocardial biochemical markers can decrease sudden death and incidence of other cardiac events.
Objective: To investigate the changes and the clinical value of biochemical markers of myocardial damage in patients undergoing operation of heart valve replacement.Methods: The creatine kinase(CK),creatine kinase-MB(CK-MB),cardiac treponin I(cTnI),C reactive protein(CRP),lactate dehydrogenase(LDH) and hydroxybutyrate dehydrogenase(HBDH) in 40 patients receiving heart valve replacement were measured before operation,instantly after extracorporeal circulation,and at 6,24,48,72 hour and 6 days after the operation,and the changes were analyzed.Results: All the biochemical markers of myocardial damage were within normal limits except CRP before operation.The figures of cTnI and CK-MB increased significantly at 6 hour after operation(P<0.01).Aspartate aminotrans ferase(AST),LDH and HBDH rose significantly at 24 hour after operation.CRP reached the peak at 48 hour after operation.The two variables of cTnI and CK-MB in serum showed a positive linear correlation and had a higher correlativity comparable to other markers.Conclusions: All the biochemical markers of myocardial damage present abnormal changes successively during the perioperation of cardiac valve replacement.Monitoring the myocardial biochemical markers can decrease sudden death and incidence of other cardiac events.
2009, 34(8): 737-739.
Abstract:
Objective: To analyze the anxiety in patients with total hip replacement during perioperation and the related psychosocial factors.Methods: The anxious status of 47 patients receiving total hip replacement were evaluated by Hamilton depression rating scale(HAMD),and the influencing factors were assessed by self-designed questionnaire and operation questionnaire.Results: Anxiety symptoms were noted in 31 cases before operation and in 17 cases after operation.The preoperative scores on HAMD were significantly higher than that in the postoperative ones.Postoperative anxiety was positively correlated to the preoperative anxiety symptoms(P<0.01).The anxiety was related to the operation,postoperative complication,joint functional recovery,cost of treatment,acknowledge to the disease and postoperative support from relatives and friends(P<0.05 to P<0.01).Conclusions: The incidence of postoperation anxiety in patients receiving total hip replacement is higher than that after operation.Patients with pre-operation anxiety symptoms have a higher risk of postoperative anxiety.The anxiety in patients with total hip replacement is correlated to the factors such as the condition of the operation,social support and so on.
Objective: To analyze the anxiety in patients with total hip replacement during perioperation and the related psychosocial factors.Methods: The anxious status of 47 patients receiving total hip replacement were evaluated by Hamilton depression rating scale(HAMD),and the influencing factors were assessed by self-designed questionnaire and operation questionnaire.Results: Anxiety symptoms were noted in 31 cases before operation and in 17 cases after operation.The preoperative scores on HAMD were significantly higher than that in the postoperative ones.Postoperative anxiety was positively correlated to the preoperative anxiety symptoms(P<0.01).The anxiety was related to the operation,postoperative complication,joint functional recovery,cost of treatment,acknowledge to the disease and postoperative support from relatives and friends(P<0.05 to P<0.01).Conclusions: The incidence of postoperation anxiety in patients receiving total hip replacement is higher than that after operation.Patients with pre-operation anxiety symptoms have a higher risk of postoperative anxiety.The anxiety in patients with total hip replacement is correlated to the factors such as the condition of the operation,social support and so on.
2009, 34(8): 743-744.
Abstract:
Objective: To investigate the effect of informational support on restlessness of patients with retained urethral catheterization in the stage of recovery from general anesthesia.Methods: Sixty esophageal cancer patients were randomly divided into two groups:The intervention group(n=30) were interviewed by the operating room nurses before operation and inducement of general anesthesia,and were given informational support about urethral catheterization besides the routine health guidance;the control group(n=30) received only the routine health guidance.The restlessness in the stage of recovery from general anesthesia was compared between the two groups.Results: The restlessness in the intervention group was much relieved compared with that in the control group(P<0.01).Conclusions: Preoperative informational support to patients with retained urethral catheterization could obviously relieve their restlessness in the recovery stage from general anesthesia.
Objective: To investigate the effect of informational support on restlessness of patients with retained urethral catheterization in the stage of recovery from general anesthesia.Methods: Sixty esophageal cancer patients were randomly divided into two groups:The intervention group(n=30) were interviewed by the operating room nurses before operation and inducement of general anesthesia,and were given informational support about urethral catheterization besides the routine health guidance;the control group(n=30) received only the routine health guidance.The restlessness in the stage of recovery from general anesthesia was compared between the two groups.Results: The restlessness in the intervention group was much relieved compared with that in the control group(P<0.01).Conclusions: Preoperative informational support to patients with retained urethral catheterization could obviously relieve their restlessness in the recovery stage from general anesthesia.
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