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2010 Vol. 35, No. 10
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2010, 35(10): 973-975.
Abstract:
Objective:To construct and identify pBiFC-VN173-Olig1 and pBiFC-VC155-Id2 eukaryotic expression plasmid for bimolecular fluorescence complementation (BiFC) assay.Methods:Rat olig1 gene from pGFP-N3-Olig1 eukaryotic expression plasmid was amplified by PCR.And rat Id2 gene from RNA of neonatal rat spinal cord was amplified by RT-PCR.The Olig1 gene was inserted into BiFC eukaryotic expression vector pBiFC-VN173 and Id2 gene was inserted into pBiFC-VC155,which were used to construct recombinant expression vector pBiFC-VN173-Olig1 and pBiFC-VC155-Id2,respectively.The recombinant vectors were identified by restriction enzyme digestion and DNA sequencing.Results:The restriction enzyme digestion and DNA sequencing results showed that the sequences and open read frames of the two vectors were completely concordant with experiment design.Conclusions:The pBiFC-VN173-Olig1 and pBiFC-VC155-Id2 were successfully constructed.These provide an experimental base for further research on interaction between Olig1 and Id2 in vivo.
Objective:To construct and identify pBiFC-VN173-Olig1 and pBiFC-VC155-Id2 eukaryotic expression plasmid for bimolecular fluorescence complementation (BiFC) assay.Methods:Rat olig1 gene from pGFP-N3-Olig1 eukaryotic expression plasmid was amplified by PCR.And rat Id2 gene from RNA of neonatal rat spinal cord was amplified by RT-PCR.The Olig1 gene was inserted into BiFC eukaryotic expression vector pBiFC-VN173 and Id2 gene was inserted into pBiFC-VC155,which were used to construct recombinant expression vector pBiFC-VN173-Olig1 and pBiFC-VC155-Id2,respectively.The recombinant vectors were identified by restriction enzyme digestion and DNA sequencing.Results:The restriction enzyme digestion and DNA sequencing results showed that the sequences and open read frames of the two vectors were completely concordant with experiment design.Conclusions:The pBiFC-VN173-Olig1 and pBiFC-VC155-Id2 were successfully constructed.These provide an experimental base for further research on interaction between Olig1 and Id2 in vivo.
2010, 35(10): 976-978.
Abstract:
Objective:To develop the hybridoma cells secreating the monoclonal antibody (McAb) against Mycobatcteium tuberculosis heat-resistant antigen (Mtb-HAg),and identify the specificity of the McAb and its biological activity.Methods:BALB/C mice were immunized 3 times with Mtb-HAg,and the spleen cells of immunized mice were fused with myeloma cells SP2/0 at the ratio of 10:1 by 50% PEG,and cultured in the HAT medium,followed by selection and subclone procedure by routine limited dilution method.The specificity and titer of the McAb were detected by ELISA method.Results:After fused 2 times,and selected and cloned 3 times,three hybridoma cell lines,Ⅰ C12,ⅡE4,and Ⅱ G8 were generated,and titers of their McAb were 1:32 000,116 000,and 116 000,respectively.ⅠC12 only had positive reaction with the Mtb-HAg protein peak Ⅰ,whereas ⅠC12 had no inhibitory effect on the Mtb-HAg activity to stimulate human γδ T cells.ⅡE4 andⅡG8 showed no positive reaction with Mtb-HAg protein peak Ⅰ,peak Ⅱ and peak Ⅲ.Conclusions:By the hybridoma technique,three hybridoma cell lines have been generated and the hybridoma Ⅰ C12 cells produce the specific McAb against Mtb-HAg protein peak I.
Objective:To develop the hybridoma cells secreating the monoclonal antibody (McAb) against Mycobatcteium tuberculosis heat-resistant antigen (Mtb-HAg),and identify the specificity of the McAb and its biological activity.Methods:BALB/C mice were immunized 3 times with Mtb-HAg,and the spleen cells of immunized mice were fused with myeloma cells SP2/0 at the ratio of 10:1 by 50% PEG,and cultured in the HAT medium,followed by selection and subclone procedure by routine limited dilution method.The specificity and titer of the McAb were detected by ELISA method.Results:After fused 2 times,and selected and cloned 3 times,three hybridoma cell lines,Ⅰ C12,ⅡE4,and Ⅱ G8 were generated,and titers of their McAb were 1:32 000,116 000,and 116 000,respectively.ⅠC12 only had positive reaction with the Mtb-HAg protein peak Ⅰ,whereas ⅠC12 had no inhibitory effect on the Mtb-HAg activity to stimulate human γδ T cells.ⅡE4 andⅡG8 showed no positive reaction with Mtb-HAg protein peak Ⅰ,peak Ⅱ and peak Ⅲ.Conclusions:By the hybridoma technique,three hybridoma cell lines have been generated and the hybridoma Ⅰ C12 cells produce the specific McAb against Mtb-HAg protein peak I.
2010, 35(10): 979-981,985.
Abstract:
Objective:To investigate the structural asymmetry of the insula and its deep structure between the left and right hemispheres.Methods:Using e-film workstation based on computer,cross-section MRI scan images of the brain among thirty cases of healthy population were chosen,images which involve the insula were impoted into photoshop 10.0 software to measure,originating the data of the distance between the area of insula and the midsagittal plane,then we come to the conclusion of 95% confidence interval of typical sections' distance variations between both sides under analysis of SPSS 13.0 statistical software.Results:On each typical sections,the mean of distances from the midsagittal plaue to central insular sulcus,central insular sulcus,anterior periinsular sulcus and inferior periinsular sulcus is dominant in 88 percent of sections in the left side.Significant left asymmetry were indentified in the central insular sulcus in which Z value from-3 to 15 mm sections,anterior periinsular sulcus in which Z value from 0 to-9 mm sections and inferior periinsular sulcus in which Z value from-9 to 9 mm sections (P<0.05-P<0.01).The variation was not significant in the remain sections of sulcus (P>0.05).Conclusions:The left insula and the area of deep structure was structural asymmetry in general pattern.Whether there is founctional asymmetry in the regional structures,and whether structural asymmetry is correlated to other regional structural and founctional asymmetry,depending further study.
Objective:To investigate the structural asymmetry of the insula and its deep structure between the left and right hemispheres.Methods:Using e-film workstation based on computer,cross-section MRI scan images of the brain among thirty cases of healthy population were chosen,images which involve the insula were impoted into photoshop 10.0 software to measure,originating the data of the distance between the area of insula and the midsagittal plane,then we come to the conclusion of 95% confidence interval of typical sections' distance variations between both sides under analysis of SPSS 13.0 statistical software.Results:On each typical sections,the mean of distances from the midsagittal plaue to central insular sulcus,central insular sulcus,anterior periinsular sulcus and inferior periinsular sulcus is dominant in 88 percent of sections in the left side.Significant left asymmetry were indentified in the central insular sulcus in which Z value from-3 to 15 mm sections,anterior periinsular sulcus in which Z value from 0 to-9 mm sections and inferior periinsular sulcus in which Z value from-9 to 9 mm sections (P<0.05-P<0.01).The variation was not significant in the remain sections of sulcus (P>0.05).Conclusions:The left insula and the area of deep structure was structural asymmetry in general pattern.Whether there is founctional asymmetry in the regional structures,and whether structural asymmetry is correlated to other regional structural and founctional asymmetry,depending further study.
2010, 35(10): 982-985.
Abstract:
Objective:To establish a method for isolation and culture of umbilical cord mesenchymal stem cells (UC-MSCs) from human umbilical cord in vitro,and explore its biological characterstics.Methods:Human UC-MSCs were isolated and expanded in culture with explantation technique and enzyme digestion (collagenase Ⅱand trypsin),respectively;the cell morphology was observed by Wright-staining;after the third passage,the surface markers of UC-MSCs were detected and idntified by flow cytometry,and the seventh passage,the cell proliferation was analyzed by MTT assay.After UC-MSCs were co-cultured with peripheral blood mononuclear cells,the impact on the T cells were observed by Hoechst and PI double staining.Results:About one week after explantation,the fibroblast-like cells sprawled out from the edge of the tissue,growing in cluster;3-5 days after enzyme digestion,cells were watched growing fiber-shaped.After passaged,UC-MSCs showed the status of long,spindle-shaped and circinate growth;they had large nucleus and clear nucleolus.Immunophenotype analysis showed that they had strong positive expression for CD29 (95.71±2.23)%,but week expression for CD31,and CD34 were (2.47±0.54)% and (3.24±0.34)%,respectively;the seventh passage UC-MSCs still had strong proliferation ability;UC-MSCs suppressed T cells proliferation in a dose-dependent manner;lymphocytes did not affect the growth of UC-MSCs.Conclusions:The cells isolated successfully from the umbilical cord have biological characteristics of the mesenchymal stem cell.
Objective:To establish a method for isolation and culture of umbilical cord mesenchymal stem cells (UC-MSCs) from human umbilical cord in vitro,and explore its biological characterstics.Methods:Human UC-MSCs were isolated and expanded in culture with explantation technique and enzyme digestion (collagenase Ⅱand trypsin),respectively;the cell morphology was observed by Wright-staining;after the third passage,the surface markers of UC-MSCs were detected and idntified by flow cytometry,and the seventh passage,the cell proliferation was analyzed by MTT assay.After UC-MSCs were co-cultured with peripheral blood mononuclear cells,the impact on the T cells were observed by Hoechst and PI double staining.Results:About one week after explantation,the fibroblast-like cells sprawled out from the edge of the tissue,growing in cluster;3-5 days after enzyme digestion,cells were watched growing fiber-shaped.After passaged,UC-MSCs showed the status of long,spindle-shaped and circinate growth;they had large nucleus and clear nucleolus.Immunophenotype analysis showed that they had strong positive expression for CD29 (95.71±2.23)%,but week expression for CD31,and CD34 were (2.47±0.54)% and (3.24±0.34)%,respectively;the seventh passage UC-MSCs still had strong proliferation ability;UC-MSCs suppressed T cells proliferation in a dose-dependent manner;lymphocytes did not affect the growth of UC-MSCs.Conclusions:The cells isolated successfully from the umbilical cord have biological characteristics of the mesenchymal stem cell.
2010, 35(10): 986-990.
Abstract:
Objective:To determine the role of vascular endothelial growth factor A (VEGFA) gene variation in the development of bicuspid aortic valve (BAV).Methods:A total of 74 unrelated pediatric patients with BAV were enrolled.Blood samples were collected from BAV patients and 300 healthy controls.All eight coding exons and exon-intron boundaries of the VEGFA gene were amplified and sequenced.Pedigree investigation was carried out by transthoracic echocardiography and corresponding sequence analysis in the patients who were confirmed to have a gene variation.Results:A heterozygous single nucleotide polymorphism (SNP) c.1039 G>A in exon 6 and a homozygous SNP c.1039 G>A in exon 6,which were not previously reported,were identified in two and one patient,respectively.These polymorphisms changed the protein sequence,replacing a valine by an isoleucine at residue 347 (p.Val347Ile) of the VEGFA protein.VEGFA 1039A allele frequency was more prevalent in BAV subjects than in controls (2.70% vs 0.33%,P<0.05).Having VEGFA 1039A allele presented increased risk for BAV (odds ratio 8.306,95% CI 1.507-45.788).For two heterozygous BAV patients,the G-to-A transition at cDNA position 1039 was transmitted from mothers.For homozygous BAV patients,8 family members were heterozygous carrier;2 family members were diagnosed as dilated aortic root,of which one was heterozygous carrier.Conclusions:The c.1039 G>A SNPs in the VEGFA may be associated with the increased risk for BAV.The dilated aortic root and BAV may share a common developmental risk factor.
Objective:To determine the role of vascular endothelial growth factor A (VEGFA) gene variation in the development of bicuspid aortic valve (BAV).Methods:A total of 74 unrelated pediatric patients with BAV were enrolled.Blood samples were collected from BAV patients and 300 healthy controls.All eight coding exons and exon-intron boundaries of the VEGFA gene were amplified and sequenced.Pedigree investigation was carried out by transthoracic echocardiography and corresponding sequence analysis in the patients who were confirmed to have a gene variation.Results:A heterozygous single nucleotide polymorphism (SNP) c.1039 G>A in exon 6 and a homozygous SNP c.1039 G>A in exon 6,which were not previously reported,were identified in two and one patient,respectively.These polymorphisms changed the protein sequence,replacing a valine by an isoleucine at residue 347 (p.Val347Ile) of the VEGFA protein.VEGFA 1039A allele frequency was more prevalent in BAV subjects than in controls (2.70% vs 0.33%,P<0.05).Having VEGFA 1039A allele presented increased risk for BAV (odds ratio 8.306,95% CI 1.507-45.788).For two heterozygous BAV patients,the G-to-A transition at cDNA position 1039 was transmitted from mothers.For homozygous BAV patients,8 family members were heterozygous carrier;2 family members were diagnosed as dilated aortic root,of which one was heterozygous carrier.Conclusions:The c.1039 G>A SNPs in the VEGFA may be associated with the increased risk for BAV.The dilated aortic root and BAV may share a common developmental risk factor.
2010, 35(10): 990-992.
Abstract:
Objective:To study the relationship between cyclophilin A (CyP A),its autoantibody and the activity of ulcerative colitis (UC).Methods:The CyP A levels of plasma and lymphocytes were detected by double sandwich ELISA in 67 active,49 inactive UC patients and 50 normal controls.Anti-CyP A autoantibody was measured by indirect ELISA.Results:The CyP A levels of plasma and lymphocytes in active UC subjects were significantly higher than those in inactive UC or normal controls (P<0.01).But there was no statistical significance between inactive UC and normal controls (P>0.05).The positive rate of anti-CyP A autoantibody in active UC patients was markedly higher than those in inactive UC or normal controls (P<0.01).Conclusions:The increase of CyP A levels of plasma and lymphocytes and the positive expression of anti-CyP A autoantibody in active UC patients are two useful markers which reflect the activity of UC.
Objective:To study the relationship between cyclophilin A (CyP A),its autoantibody and the activity of ulcerative colitis (UC).Methods:The CyP A levels of plasma and lymphocytes were detected by double sandwich ELISA in 67 active,49 inactive UC patients and 50 normal controls.Anti-CyP A autoantibody was measured by indirect ELISA.Results:The CyP A levels of plasma and lymphocytes in active UC subjects were significantly higher than those in inactive UC or normal controls (P<0.01).But there was no statistical significance between inactive UC and normal controls (P>0.05).The positive rate of anti-CyP A autoantibody in active UC patients was markedly higher than those in inactive UC or normal controls (P<0.01).Conclusions:The increase of CyP A levels of plasma and lymphocytes and the positive expression of anti-CyP A autoantibody in active UC patients are two useful markers which reflect the activity of UC.
2010, 35(10): 993-995.
Abstract:
Objective:To explore the diagnostic value of detection of survivin combined with cytokeratin 19 fragments antigen,21-1 (CYFRA21-1) and carcinoembryonic antigen (CEA) in pleural effusions caused by lung cancer.Methods:Reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the survivin mRNA expression in pleural effusion of 65 patients caused by lung cancer and 58 patients caused by tuberculous pleurisy,and the cytological examinations in these samples were determined.The detections of CEA and CYERA21-1 were performed by radioimmunoassay.Results:The positive rate of survivin mRNA was 80.0% in pleural effusions of lung cancer,which was significantly higher than the positive rate of initial cytological examination (47.6%).And there was no survivin mRNA expression in tuberculous pleural effusions (P<0.01).The specificity of survivin mRNA in pleural effusions caused by lung cancer (94.7%) was higher than the specificity of CEA and CYFRA21-1 (77.6% and 75.8%,respectively) (P<0.05).The sensitivity of survivin mRNA detection (80.0%) was significantly higher than CEA detection (50.8%) (P<0.05),but there was no significant difference between survivin mRNA and CYFRA21-1 detection (P>0.05).Conclusions:Survivin mRNA was a specific molecular marker for diagnosis of pleural metastasis of lung cancer,which had significant implications for diagnosis and differential diagnosis of lung cancer and tuberculous pleurisy combined with CYFRA21-1 and CEA.
Objective:To explore the diagnostic value of detection of survivin combined with cytokeratin 19 fragments antigen,21-1 (CYFRA21-1) and carcinoembryonic antigen (CEA) in pleural effusions caused by lung cancer.Methods:Reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the survivin mRNA expression in pleural effusion of 65 patients caused by lung cancer and 58 patients caused by tuberculous pleurisy,and the cytological examinations in these samples were determined.The detections of CEA and CYERA21-1 were performed by radioimmunoassay.Results:The positive rate of survivin mRNA was 80.0% in pleural effusions of lung cancer,which was significantly higher than the positive rate of initial cytological examination (47.6%).And there was no survivin mRNA expression in tuberculous pleural effusions (P<0.01).The specificity of survivin mRNA in pleural effusions caused by lung cancer (94.7%) was higher than the specificity of CEA and CYFRA21-1 (77.6% and 75.8%,respectively) (P<0.05).The sensitivity of survivin mRNA detection (80.0%) was significantly higher than CEA detection (50.8%) (P<0.05),but there was no significant difference between survivin mRNA and CYFRA21-1 detection (P>0.05).Conclusions:Survivin mRNA was a specific molecular marker for diagnosis of pleural metastasis of lung cancer,which had significant implications for diagnosis and differential diagnosis of lung cancer and tuberculous pleurisy combined with CYFRA21-1 and CEA.
2010, 35(10): 996-999.
Abstract:
Objective:To investigate the clinicopathological features of epithelioid trophoblastic tumor (ETT) of the uterus with emphasis on its differential diagnosis.Methods:The clinicopathological data of 1 case of ETT was analyzed and the literature was reviewed.Results:The patient was a 30-year-old female with the symptoms of delayed menstruation and abnormal vaginal bleeding for half a year.Gross examination of the hysterectomy specimen revealed a limit-unclear lesion involving the left uterine wall.The tumor was 5 cm in size and was necrotic with areas of hemorrhage;microscopically,the tumor was composed of one type intermediate trophoblast cells arranged in nests and cords,which distributed in a geographical pattern with hyalinization or eosinophilic debris of tumor cell necrosis.The medium-sized tumor cells contained abundant eosinophilic or clear cytoplasm and distinct cell borders,round or oval nuclei with distinct nucleoli.Immunohistochemically,the tumor cells were positive for AE1/AE3,p63 and Ki-67,and partially positive for hCG.Conclusions:ETT is a rare gestational trophoblastic tumor that has malignant biological behavior of lower grade.It should be differentiated from cervical squamous cell carcinoma and other trophoblastic tumors.
Objective:To investigate the clinicopathological features of epithelioid trophoblastic tumor (ETT) of the uterus with emphasis on its differential diagnosis.Methods:The clinicopathological data of 1 case of ETT was analyzed and the literature was reviewed.Results:The patient was a 30-year-old female with the symptoms of delayed menstruation and abnormal vaginal bleeding for half a year.Gross examination of the hysterectomy specimen revealed a limit-unclear lesion involving the left uterine wall.The tumor was 5 cm in size and was necrotic with areas of hemorrhage;microscopically,the tumor was composed of one type intermediate trophoblast cells arranged in nests and cords,which distributed in a geographical pattern with hyalinization or eosinophilic debris of tumor cell necrosis.The medium-sized tumor cells contained abundant eosinophilic or clear cytoplasm and distinct cell borders,round or oval nuclei with distinct nucleoli.Immunohistochemically,the tumor cells were positive for AE1/AE3,p63 and Ki-67,and partially positive for hCG.Conclusions:ETT is a rare gestational trophoblastic tumor that has malignant biological behavior of lower grade.It should be differentiated from cervical squamous cell carcinoma and other trophoblastic tumors.
2010, 35(10): 1000-1001.
Abstract:
Objective:To evaluate the clinical effect of manual half-intracapsula chop technique in small incision non-phacoe mulsification cataract surgery (SICS).Methods:Twenty-six cases (27 eyes) of cataract with above grade-Ⅲ nucleus were performed conventional small incision cataract surgery using manual half-intracapsula chop technique.Each nucleus was separated into two or three pieces which were taken out one by one;intraocular lens was implanted in each eye.Results:The necli were successfully separated in 27 eyes with no posterior capsular rupture.After the operation,slight cornea edema was observed in 7 eyes and slight massive opacity of the endothelium in 2 cases.The visual acuity of 20 eyes was improved to 0.5 or over one day after the operation,among which more than 8 eyes reached 0.8.The visual acuity of 23 eyes was improved to 0.5 or over one week after the operation,among which 10 eyes or over reached 0.8.Conclusions:Manual half-intracapsula chop technique can expand the operation space and reduce the lesion to coneal endothelium.It is a safe method for treatment of patients with cataract.
Objective:To evaluate the clinical effect of manual half-intracapsula chop technique in small incision non-phacoe mulsification cataract surgery (SICS).Methods:Twenty-six cases (27 eyes) of cataract with above grade-Ⅲ nucleus were performed conventional small incision cataract surgery using manual half-intracapsula chop technique.Each nucleus was separated into two or three pieces which were taken out one by one;intraocular lens was implanted in each eye.Results:The necli were successfully separated in 27 eyes with no posterior capsular rupture.After the operation,slight cornea edema was observed in 7 eyes and slight massive opacity of the endothelium in 2 cases.The visual acuity of 20 eyes was improved to 0.5 or over one day after the operation,among which more than 8 eyes reached 0.8.The visual acuity of 23 eyes was improved to 0.5 or over one week after the operation,among which 10 eyes or over reached 0.8.Conclusions:Manual half-intracapsula chop technique can expand the operation space and reduce the lesion to coneal endothelium.It is a safe method for treatment of patients with cataract.
2010, 35(10): 1002-1003.
Abstract:
Objective:To summarize the experience of the microsurgical treatment of sphenoid ridge meningioma.Methods:The clinical data of 38 patients with sphenoid ridge meningioma who underwent microsurgical treatment were analyzed retrospectively.Results:Of 38 cases of sphenoid ridge meningioma,34 were totally removed (Grade Ⅰ,Ⅱ of Simpson system),4 subtotally (Grade Ⅲ,Ⅳ of Simpson system),2 cases with hemiparalysis.No postoperative mortality was found.Conclusions:Proper preservation of vital vessel and nerves can improve the removal rate of sphenoid ridge meningioma and reduce postoperative nervous malfunction.
Objective:To summarize the experience of the microsurgical treatment of sphenoid ridge meningioma.Methods:The clinical data of 38 patients with sphenoid ridge meningioma who underwent microsurgical treatment were analyzed retrospectively.Results:Of 38 cases of sphenoid ridge meningioma,34 were totally removed (Grade Ⅰ,Ⅱ of Simpson system),4 subtotally (Grade Ⅲ,Ⅳ of Simpson system),2 cases with hemiparalysis.No postoperative mortality was found.Conclusions:Proper preservation of vital vessel and nerves can improve the removal rate of sphenoid ridge meningioma and reduce postoperative nervous malfunction.
2010, 35(10): 1004-1006.
Abstract:
Objective:To compare the clinical effects of Lichtenstein,Rutkow and Millikan procedures on inguinal hernia repair.Methods:Thirty-eight patients received mesh tension-free hernioplasty (Lichtenstein procedure),forty-seven patients mesh-plug tension-free hernioplasty (Rutkow procedure) and twenty-eight patients modified mesh-plug tension-free hernioplasty (Millikan procedure).The operation time,postoperative pain,time of hospital stay,time to return to work,cost,postoperative complication and recurrent rate were compared among the three groups.Results:There was no significant difference in operation time,postoperative pain,time of hospital stay,time to return to work,postoperative complication and recurrent rate among the three groups (P>0.05).However,Lichtenstein group had a significantly lower cost than the other two groups (P<0.01).Rutkow group had a higher incidence of foreign body sensation than the other two groups (P<0.01).Conclusions:The three types of tension-free hernioplasty are all effective in treatment of primary monolateral inguinal hernia.Lichtenstein hernioplasty is more comfortable and cost-effective,and so is the choice procedure for repair of primary inguinal hernia.
Objective:To compare the clinical effects of Lichtenstein,Rutkow and Millikan procedures on inguinal hernia repair.Methods:Thirty-eight patients received mesh tension-free hernioplasty (Lichtenstein procedure),forty-seven patients mesh-plug tension-free hernioplasty (Rutkow procedure) and twenty-eight patients modified mesh-plug tension-free hernioplasty (Millikan procedure).The operation time,postoperative pain,time of hospital stay,time to return to work,cost,postoperative complication and recurrent rate were compared among the three groups.Results:There was no significant difference in operation time,postoperative pain,time of hospital stay,time to return to work,postoperative complication and recurrent rate among the three groups (P>0.05).However,Lichtenstein group had a significantly lower cost than the other two groups (P<0.01).Rutkow group had a higher incidence of foreign body sensation than the other two groups (P<0.01).Conclusions:The three types of tension-free hernioplasty are all effective in treatment of primary monolateral inguinal hernia.Lichtenstein hernioplasty is more comfortable and cost-effective,and so is the choice procedure for repair of primary inguinal hernia.
2010, 35(10): 1007-1008.
Abstract:
Objective:To evaluate the therapeutic effect of laparoscopy high ligation with two-trocars on varicocele.Methods:Seventy-six patients with varicocele were treated by laparoscopy high ligation with two-trocars.Results:The operation was successful in all the cases.The operation time was 6-20 min,and the hospital stay was 3-5 days.During the follow-up of 1 to 12 months,no testicular atrophy or recurrence was observed.Conclusions:The procedure of laparoscopy high ligation with two-trocars is safe and effective for treatment of patients with varicocele,especially for those with recurrent or bilateral varicocele.
Objective:To evaluate the therapeutic effect of laparoscopy high ligation with two-trocars on varicocele.Methods:Seventy-six patients with varicocele were treated by laparoscopy high ligation with two-trocars.Results:The operation was successful in all the cases.The operation time was 6-20 min,and the hospital stay was 3-5 days.During the follow-up of 1 to 12 months,no testicular atrophy or recurrence was observed.Conclusions:The procedure of laparoscopy high ligation with two-trocars is safe and effective for treatment of patients with varicocele,especially for those with recurrent or bilateral varicocele.
2010, 35(10): 1009-1011.
Abstract:
Objective:To demonstrate the efficacy,safety and appropriate mode of inserting a pleural catheter for thrombin in treatment of malignant pleural effusion.Methods:With the guidance of ultrasound,32 patients with malignant pleural effusion were inserted implantable pleural catheter for instillation of thrombin,and the result was compared with that of 36 cases treated by conventional chest puncture drainage and 29 cases by traditional closed thoracic drainage.Results:In the ARROW central venous catheter group,the number of symptom relief (13/3),total effective rate (90.6%),pleural adhesion rate (62.5%),percentage of pleural effusion decrease after treatment (76±6.3)%,drainage volume of fluid (4 900±150) ml,treatment time (5±1.2) days and hospital stay (17±2.9) days all surpassed those of the conventional chest puncture drainage group (P<0.01).The central venous catheter group had less complication,less liquid drainage and shorter hospital stay than the traditional closed thoracic drainage group (P<0.05~P<0.01).Conclusions:The insertion of a central venous catheter for instillation of thrombin through a chest drain T tube is simple,effective and safe for treatment of patients with malignant pleural effusion,which accepted welcome by majority of the patients.
Objective:To demonstrate the efficacy,safety and appropriate mode of inserting a pleural catheter for thrombin in treatment of malignant pleural effusion.Methods:With the guidance of ultrasound,32 patients with malignant pleural effusion were inserted implantable pleural catheter for instillation of thrombin,and the result was compared with that of 36 cases treated by conventional chest puncture drainage and 29 cases by traditional closed thoracic drainage.Results:In the ARROW central venous catheter group,the number of symptom relief (13/3),total effective rate (90.6%),pleural adhesion rate (62.5%),percentage of pleural effusion decrease after treatment (76±6.3)%,drainage volume of fluid (4 900±150) ml,treatment time (5±1.2) days and hospital stay (17±2.9) days all surpassed those of the conventional chest puncture drainage group (P<0.01).The central venous catheter group had less complication,less liquid drainage and shorter hospital stay than the traditional closed thoracic drainage group (P<0.05~P<0.01).Conclusions:The insertion of a central venous catheter for instillation of thrombin through a chest drain T tube is simple,effective and safe for treatment of patients with malignant pleural effusion,which accepted welcome by majority of the patients.
2010, 35(10): 1012-1014,1017.
Abstract:
Objective:To investigate the pathologic feature,immunophenotype and particularity of neurofibromatosis type 1 (NF1) accompanied with multiple gastrointestinal stromal of tumors (GIST).Methods:The clinical manifestation,histopathology and immunophenotype of the case of NF1 accompanied with GIST were analysed,and reviewed of the literatwre was reviwed.Results:The patient was a 46-year-old woman with a history of cutaneous nodules for 28 years,did not take any treatment.When she pregnanted after marriage,the cutaneous nodules number increased,and involving the face,trunk and limbs for over the entire skin surface.Recently,paroxysmal pain in the lower abdomen was appeared.The ultrasound revealed substantial masses in the abdomen.The subserosal nodules in the jejunum and the ileum were found at surgery,about a dozen more.The partial jejunum and the subserosal nodules (2 tumors) were resected.At the time of the procedure,one of the cutaneous lesions was also sampled.Tumor cells of the jejunum and ileum were rich in spindle-shaped or oval,small nucleoli,mitotic figures to be seen.Immunophenotype:(1) tumors located in the jejunum and ileum were positive for vimentin (3+),CD117 (2+),CD34 (2+),MSA (1+),negative for S-100 protein,low expression of Ki-67; (2) skin nodule on the left forearm:S-100 protein (3+),CD117 (-),CD34 (-),MSA (-).No chemoradiation therapy after resection,at the 7th month,the patient was in good condition.Conclusions:NF1 associated GIST typically involves the small intestine and may be multifocal.The tumors are spindle cell in type with cellularity,low mitotic activity and low-grade.
Objective:To investigate the pathologic feature,immunophenotype and particularity of neurofibromatosis type 1 (NF1) accompanied with multiple gastrointestinal stromal of tumors (GIST).Methods:The clinical manifestation,histopathology and immunophenotype of the case of NF1 accompanied with GIST were analysed,and reviewed of the literatwre was reviwed.Results:The patient was a 46-year-old woman with a history of cutaneous nodules for 28 years,did not take any treatment.When she pregnanted after marriage,the cutaneous nodules number increased,and involving the face,trunk and limbs for over the entire skin surface.Recently,paroxysmal pain in the lower abdomen was appeared.The ultrasound revealed substantial masses in the abdomen.The subserosal nodules in the jejunum and the ileum were found at surgery,about a dozen more.The partial jejunum and the subserosal nodules (2 tumors) were resected.At the time of the procedure,one of the cutaneous lesions was also sampled.Tumor cells of the jejunum and ileum were rich in spindle-shaped or oval,small nucleoli,mitotic figures to be seen.Immunophenotype:(1) tumors located in the jejunum and ileum were positive for vimentin (3+),CD117 (2+),CD34 (2+),MSA (1+),negative for S-100 protein,low expression of Ki-67; (2) skin nodule on the left forearm:S-100 protein (3+),CD117 (-),CD34 (-),MSA (-).No chemoradiation therapy after resection,at the 7th month,the patient was in good condition.Conclusions:NF1 associated GIST typically involves the small intestine and may be multifocal.The tumors are spindle cell in type with cellularity,low mitotic activity and low-grade.
2010, 35(10): 1015-1017.
Abstract:
Objective:To observe the clinical efficacy and safety of peginterferon α-2a in treatment of chronic hepatitis B.Methods:Two patients with chronic hepatitis B were treated with peginterferon α-2a;and the blood,HBV viral markers,HBV-DNA and liver function were tested regularly during the therapy.Results:One patient demonstrated negative HBeAg at 12 weeks,HBeAg seroconversion at 48 weeks,and HBV-DNA was undetectable at the same time.The other patient had undetectable HBV-DNA at 6 weeks,negative HBsAg at 12 weeks,and HBeAg seroconversion at 24 weeks.Conclusions:Peginterferon α-2a can regulate the immune response and obtain serum e/s antigens clearance or conversion in a limited treatment course.Peginterferon α-2a is effective on patient with chronic hepatitis B.
Objective:To observe the clinical efficacy and safety of peginterferon α-2a in treatment of chronic hepatitis B.Methods:Two patients with chronic hepatitis B were treated with peginterferon α-2a;and the blood,HBV viral markers,HBV-DNA and liver function were tested regularly during the therapy.Results:One patient demonstrated negative HBeAg at 12 weeks,HBeAg seroconversion at 48 weeks,and HBV-DNA was undetectable at the same time.The other patient had undetectable HBV-DNA at 6 weeks,negative HBsAg at 12 weeks,and HBeAg seroconversion at 24 weeks.Conclusions:Peginterferon α-2a can regulate the immune response and obtain serum e/s antigens clearance or conversion in a limited treatment course.Peginterferon α-2a is effective on patient with chronic hepatitis B.
2010, 35(10): 1018-1020.
Abstract:
Objective:To observe the effect of atorvastatin on creactive protein (CRP),monocyte chemoattractant protein-1 (MCP-1) and urinary albumin excretion rate (UAER) in patients with diabetic nephropathy (DN).Methods:One hundred and eight DN patients were randomly divided into treatment group (54 cases) and atorvastatin group (54 cases).The treatment group were administered the routine therapy and the atorvastatin group received atorvastatin 40 mg daily.The therapies lasted for 12 weeks in both groups.Twenty healthy people acted as control.Serum CRP,MCP-1,glucose,lipids levels and UAER were compared between the two groups before treatment and 4,8,12 weeks after treatment.Results:Levels of plasma CRP,MCP-1 and UAER had no obvious difference between the two groups before treatment (P>0.05),but the above levels decreased significant in atorvastatin group after the therapy (P<0.01).Serum CRP,MCP-1 and UAER presented a positive correlation (P<0.01).Conclusions:Atorvastatin could effectively decrease the urinary protein excretion by restraining inflammation in DN patients.
Objective:To observe the effect of atorvastatin on creactive protein (CRP),monocyte chemoattractant protein-1 (MCP-1) and urinary albumin excretion rate (UAER) in patients with diabetic nephropathy (DN).Methods:One hundred and eight DN patients were randomly divided into treatment group (54 cases) and atorvastatin group (54 cases).The treatment group were administered the routine therapy and the atorvastatin group received atorvastatin 40 mg daily.The therapies lasted for 12 weeks in both groups.Twenty healthy people acted as control.Serum CRP,MCP-1,glucose,lipids levels and UAER were compared between the two groups before treatment and 4,8,12 weeks after treatment.Results:Levels of plasma CRP,MCP-1 and UAER had no obvious difference between the two groups before treatment (P>0.05),but the above levels decreased significant in atorvastatin group after the therapy (P<0.01).Serum CRP,MCP-1 and UAER presented a positive correlation (P<0.01).Conclusions:Atorvastatin could effectively decrease the urinary protein excretion by restraining inflammation in DN patients.
2010, 35(10): 1021-1023.
Abstract:
Objective:To summarize the dignosis and treatment of pancreaticoduodenectomy (PD) for gastric cancer after operation.Methods:From Jan.1998 to Jan.2008,6 patients performed with PD after operation because of gastric cancer were retrospectly analysed.Of the 6 cases,5 cases were porformed end-side-mucosa PD,1 end-end PD.Results:None of the cases died periopratively.Postoperative complications,including lung injection in 1 case,pancreatic leakage in 1 case,injection of abdomino in 1 case,were cured by conservative treatment.Conclusions:PD is a safe and appropriate surgical option for patients with the head of pancreas invasive by gastric cancer or local recurrence after gastrectomy for gastric cancer if without distant metastasis.
Objective:To summarize the dignosis and treatment of pancreaticoduodenectomy (PD) for gastric cancer after operation.Methods:From Jan.1998 to Jan.2008,6 patients performed with PD after operation because of gastric cancer were retrospectly analysed.Of the 6 cases,5 cases were porformed end-side-mucosa PD,1 end-end PD.Results:None of the cases died periopratively.Postoperative complications,including lung injection in 1 case,pancreatic leakage in 1 case,injection of abdomino in 1 case,were cured by conservative treatment.Conclusions:PD is a safe and appropriate surgical option for patients with the head of pancreas invasive by gastric cancer or local recurrence after gastrectomy for gastric cancer if without distant metastasis.
2010, 35(10): 1025-1027.
Abstract:
Objective:To improve the curative effect of microsurgery in treatment of patients with meningiomas in the middle cortex areas.Methods:The clinical data of 24 cases of meningiomas in the middle cortex areas which had been confirmed by surgery and pathology were retrospectively analyzed.Results:Simpson grade Ⅰresection was achieved in 11 cases,grade Ⅱ in 12 cases and grade Ⅲ in 1 case.No patients died during the operation.All the cases were followed up for 3 months to 8 years.No cases with Simpson gradeⅠresection recurred,2 cases with Simpson grade Ⅱ resection relapsed and received the second operation,and the one case with Simpson grade Ⅲ resection did not have obvious change in size of the tumor during the follow-up.Conclusions:Microsurgical technique may elevate the total tumor resection rate in treatment of meningiomas in the middle cortex areas,decrease the injury in the important functional area of the brain,reduce complications and improve the patients' life quality.
Objective:To improve the curative effect of microsurgery in treatment of patients with meningiomas in the middle cortex areas.Methods:The clinical data of 24 cases of meningiomas in the middle cortex areas which had been confirmed by surgery and pathology were retrospectively analyzed.Results:Simpson grade Ⅰresection was achieved in 11 cases,grade Ⅱ in 12 cases and grade Ⅲ in 1 case.No patients died during the operation.All the cases were followed up for 3 months to 8 years.No cases with Simpson gradeⅠresection recurred,2 cases with Simpson grade Ⅱ resection relapsed and received the second operation,and the one case with Simpson grade Ⅲ resection did not have obvious change in size of the tumor during the follow-up.Conclusions:Microsurgical technique may elevate the total tumor resection rate in treatment of meningiomas in the middle cortex areas,decrease the injury in the important functional area of the brain,reduce complications and improve the patients' life quality.
Efficacy and safety of phenobarbital in preventing intraventricular hemorrhage in premature newborns
2010, 35(10): 1030-1032.
Abstract:
Objective:To investigate the therapeutic efficacy and safety of phenobarbital in preventing premature newborns from intraventricular hemorrhage (IVH).Methods:Eighty-six premature newborns (≤ 35 weeks gestation and <6 hours post partum) were divided into treatment group (hospitalized in the singular month,n=49) and control group (hospitalized in the even month,n=37).Both groups received the same routine therapy.The patients in the treatment group were administered phenobarbital (20 mg/kg twice via intravenous drip) immediately after admission,followed by a course of 5 days' maintenance dosage (5 mg/kg intravenous drip with 12 hours interval) after 24 hours.The incidences of IVH,severe IVH,respiratory arrest and low response were compared between the two groups.Results:The phenobarbital treatment group had a significantly lower incidence of IVH (20.4%) than the control group (37.8%) (P<0.05).However,there was no difference in the incidence of severe IVH,respiratory arrest or low response between the treatment group (4.1%,34.7% and 24.5%,respectively) and the control group (8.1%,40.5% and 21.6%,respectively).Conclusions:Phenobarbital can effectively and safely prevent the incidence of IVH in premature newborns,and leaves no significant side effects.
Objective:To investigate the therapeutic efficacy and safety of phenobarbital in preventing premature newborns from intraventricular hemorrhage (IVH).Methods:Eighty-six premature newborns (≤ 35 weeks gestation and <6 hours post partum) were divided into treatment group (hospitalized in the singular month,n=49) and control group (hospitalized in the even month,n=37).Both groups received the same routine therapy.The patients in the treatment group were administered phenobarbital (20 mg/kg twice via intravenous drip) immediately after admission,followed by a course of 5 days' maintenance dosage (5 mg/kg intravenous drip with 12 hours interval) after 24 hours.The incidences of IVH,severe IVH,respiratory arrest and low response were compared between the two groups.Results:The phenobarbital treatment group had a significantly lower incidence of IVH (20.4%) than the control group (37.8%) (P<0.05).However,there was no difference in the incidence of severe IVH,respiratory arrest or low response between the treatment group (4.1%,34.7% and 24.5%,respectively) and the control group (8.1%,40.5% and 21.6%,respectively).Conclusions:Phenobarbital can effectively and safely prevent the incidence of IVH in premature newborns,and leaves no significant side effects.
2010, 35(10): 1040-1042.
Abstract:
Objective:To research into the mental health of freshmen,so as to provide basis for the psychological education.Methods:A total of 3 775 freshmen were questionaired by university personality inventory (UPI).Results:Two hundred and forty students were classified as level one;the item 14,52,58,9 and 22 acquired the highest selectance;37 students had suicide idea.The UPI score in male students was significantly lower than that in female.Among the 54 students of level A,3 demonstrated serious psychological problems,43 common psychological problems and 8 were in healthy state.Conclusions:The majority of freshmen present healthy psychology.The negative emotions include depression,obsession,inferiority and anxiety,and the female students require special attention.The freshmen are in urgent need of psychological guidance.
Objective:To research into the mental health of freshmen,so as to provide basis for the psychological education.Methods:A total of 3 775 freshmen were questionaired by university personality inventory (UPI).Results:Two hundred and forty students were classified as level one;the item 14,52,58,9 and 22 acquired the highest selectance;37 students had suicide idea.The UPI score in male students was significantly lower than that in female.Among the 54 students of level A,3 demonstrated serious psychological problems,43 common psychological problems and 8 were in healthy state.Conclusions:The majority of freshmen present healthy psychology.The negative emotions include depression,obsession,inferiority and anxiety,and the female students require special attention.The freshmen are in urgent need of psychological guidance.
2010, 35(10): 1043-1045.
Abstract:
Objective:To investigate the means of preparing 2-pyridylboronic acid.Methods:The product was synthesized with 2-bromopyridine,tris-trimethylsilylborate and iPrMgCl as Grignard reagents under N2,and identified by 1H-NMR.Results:2-pyridylboronic acid was synthesized with the yield of 67.7% on conditions of n (tris-trimethylsilylborate):n (2-bromopyridine)=2:1;the reaction temperature was 0℃,the reaction time 3 hours and the hydrolysis temperature 0℃.Conclusions:2-pyridylboronic acid can be synthesized by Grignard reaction on moderate conditions with high yield,which may contribute to the study of pyridine drugs.
Objective:To investigate the means of preparing 2-pyridylboronic acid.Methods:The product was synthesized with 2-bromopyridine,tris-trimethylsilylborate and iPrMgCl as Grignard reagents under N2,and identified by 1H-NMR.Results:2-pyridylboronic acid was synthesized with the yield of 67.7% on conditions of n (tris-trimethylsilylborate):n (2-bromopyridine)=2:1;the reaction temperature was 0℃,the reaction time 3 hours and the hydrolysis temperature 0℃.Conclusions:2-pyridylboronic acid can be synthesized by Grignard reaction on moderate conditions with high yield,which may contribute to the study of pyridine drugs.
2010, 35(10): 1046-1047,1050.
Abstract:
Objective:To study the effect of laccrocapram on percutaneous absorption of aspirin cream across mice skin in vitro.Methods:Fluids were collected by using Franz diffusion cells after permeation through the skin of mice.And the per square centimeter's accumulative permeation quantity,steady percutaneous speed and permeation coefficient were determined by ultra-violet spectroscopy.Results:Steady percutaneous speed of acetyl salicylic acid cream with 5% and 10% laccrocapram increased 10.6% and 25.0%,respectively.Conclusions:Different concentrations of laccrocapram can increasing permeation of acetyl salicylic acid.The permeation from emulsion base conformed to Higuchi equation.
Objective:To study the effect of laccrocapram on percutaneous absorption of aspirin cream across mice skin in vitro.Methods:Fluids were collected by using Franz diffusion cells after permeation through the skin of mice.And the per square centimeter's accumulative permeation quantity,steady percutaneous speed and permeation coefficient were determined by ultra-violet spectroscopy.Results:Steady percutaneous speed of acetyl salicylic acid cream with 5% and 10% laccrocapram increased 10.6% and 25.0%,respectively.Conclusions:Different concentrations of laccrocapram can increasing permeation of acetyl salicylic acid.The permeation from emulsion base conformed to Higuchi equation.
2010, 35(10): 1048-1050.
Abstract:
Objective:To investigate X-ray findings and its clinical value of diabetic foot.Methods:X-ray findings and clinical data of 50 patients with diabetic foot were analyzed retrospectively.Results:X-ray findings showed 48 cases with osteoporosis,25 cases with bone damage,23 cases with joint surface damage,5 cases with backbone of the symmetry of atrophy,7 cases with Charcot's joint,4 cases with increased density of bone marrow and 21 cases with adjacent soft tissue changes.Conclusions:There are some special characteristics of diabetic foot on the X-ray findings,which have the very important significance for the diabetes diagnosis,disease evaluation and guide for clinical treatment combined with clinical behaviors.
Objective:To investigate X-ray findings and its clinical value of diabetic foot.Methods:X-ray findings and clinical data of 50 patients with diabetic foot were analyzed retrospectively.Results:X-ray findings showed 48 cases with osteoporosis,25 cases with bone damage,23 cases with joint surface damage,5 cases with backbone of the symmetry of atrophy,7 cases with Charcot's joint,4 cases with increased density of bone marrow and 21 cases with adjacent soft tissue changes.Conclusions:There are some special characteristics of diabetic foot on the X-ray findings,which have the very important significance for the diabetes diagnosis,disease evaluation and guide for clinical treatment combined with clinical behaviors.
2010, 35(10): 1051-1052,1055.
Abstract:
Objective:To investigate the diagnostic value of advanced dynamic flow (ADF) in incomplete abortion induced by drugs.Methods:One hundred and twenty patients who were still bleeding after abortion induced by drugs for more than two weeks were examined by transvaginal color doppler flow imaging (CDFI) and ADF.And the outcome of 59 cases was compared with that by histological examinations after uterine curettage.Results:The sensitivity,specificity and accuracy by ADF were 91.49%,83.33% and 89.83%,respectively,and the positive and negative predictive values reached 95.56% and 71.43%,which were higher than the 68.09%,66.67%,67.80%,88.89% and 34.78% by CDFI.The accuracy by ADF was higher than CDFI (P<0.05).Conclusions:ADF is of great value in the diagnosis of incomplete abortion induced by drugs.
Objective:To investigate the diagnostic value of advanced dynamic flow (ADF) in incomplete abortion induced by drugs.Methods:One hundred and twenty patients who were still bleeding after abortion induced by drugs for more than two weeks were examined by transvaginal color doppler flow imaging (CDFI) and ADF.And the outcome of 59 cases was compared with that by histological examinations after uterine curettage.Results:The sensitivity,specificity and accuracy by ADF were 91.49%,83.33% and 89.83%,respectively,and the positive and negative predictive values reached 95.56% and 71.43%,which were higher than the 68.09%,66.67%,67.80%,88.89% and 34.78% by CDFI.The accuracy by ADF was higher than CDFI (P<0.05).Conclusions:ADF is of great value in the diagnosis of incomplete abortion induced by drugs.
2010, 35(10): 1053-1055.
Abstract:
Objective:To discuss the MRI findings and differential diagnosis of spinal epidural cavernous hemangioma.Methods:Six cases of spinal epidural cavernous hemangioma confirmed by operation and pathology were retrospectively analyzed and compared with other epidural neoplasm.Results:The masses in the six cases showed hyperintensity signal on T2-weighted images,iso-or hyperintensity signal on T1-weighted images and homogeneous strong enhancement.The lesions were at the thoracic level (n=5) and cervical-thoracic level (n=1).The masses were located in the epidural space and extended parallel with the long axis of the spine.Two cases were at the back of the spinal cord,4 cases were at the left or right side back of the spinal cord;and the adjacent intervertebral neural foramen were widened.Conclusions:Spinal epidural cavernous hemangioma is rarely seen in epidural space and have some MRI features.MRI is the most reliable examination means before operation,but definite diagnosis depends on clinical and pathological features.
Objective:To discuss the MRI findings and differential diagnosis of spinal epidural cavernous hemangioma.Methods:Six cases of spinal epidural cavernous hemangioma confirmed by operation and pathology were retrospectively analyzed and compared with other epidural neoplasm.Results:The masses in the six cases showed hyperintensity signal on T2-weighted images,iso-or hyperintensity signal on T1-weighted images and homogeneous strong enhancement.The lesions were at the thoracic level (n=5) and cervical-thoracic level (n=1).The masses were located in the epidural space and extended parallel with the long axis of the spine.Two cases were at the back of the spinal cord,4 cases were at the left or right side back of the spinal cord;and the adjacent intervertebral neural foramen were widened.Conclusions:Spinal epidural cavernous hemangioma is rarely seen in epidural space and have some MRI features.MRI is the most reliable examination means before operation,but definite diagnosis depends on clinical and pathological features.
2010, 35(10): 1056-1058.
Abstract:
Objective:To analyze the employment status of the graduate students in the preventive medicine major and the influencing factors,and to discuss how to ensure the training quality to acquire better employment.Methods:The employment data of the graduates in the preventive medicine major from 2005-2009 in Bengbu Medical College were collected and analyzed.Results:For 5 years,the first-time and year-end employment rates of the Preventive Medicine graduates maintained a high levels,and were to 87.86% and 100.00%,respectively.They were distributed in a wide range of areas:51.88% of them found a job in the coastal developed areas,such as Jiangsu,Zhejiang and Shanghai,and mainly took a position in the Center for Disease Control,the supervision Bureaus or hospitals (community health service centers).The ratio of the graduates proceeding to the master's degree was increasing year by year.Conclusions:The employment status is satisfactory for the graduates in the preventive medicine major.Further efforts should still be made for better future.
Objective:To analyze the employment status of the graduate students in the preventive medicine major and the influencing factors,and to discuss how to ensure the training quality to acquire better employment.Methods:The employment data of the graduates in the preventive medicine major from 2005-2009 in Bengbu Medical College were collected and analyzed.Results:For 5 years,the first-time and year-end employment rates of the Preventive Medicine graduates maintained a high levels,and were to 87.86% and 100.00%,respectively.They were distributed in a wide range of areas:51.88% of them found a job in the coastal developed areas,such as Jiangsu,Zhejiang and Shanghai,and mainly took a position in the Center for Disease Control,the supervision Bureaus or hospitals (community health service centers).The ratio of the graduates proceeding to the master's degree was increasing year by year.Conclusions:The employment status is satisfactory for the graduates in the preventive medicine major.Further efforts should still be made for better future.
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