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2010 Vol. 35, No. 12
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2010, 35(12): 1197-1199.
Abstract:
Objective: To isolate and purify recombinant Schistosoma japonicum (Chinese strain) protein glutathione S-transferase (rSjGST) for preparing the monoclonal antibodies (McAbs) against rSjGST.Methods: Balb/c mice were immunized with purified rSjGST protein.The anti-rSjGST monoclonal antibody was obtained through hybridoma technique.Characterization of the antibody was performed by ELISA and Western blot analysis.Results: The data had shown that high purified rSjGST protein and the hybridoma cell line 3C12 secreting McAbs against rSjGST protein were obtained.The subclass of McAbs was confirmed to be IgG1.Conclusions: The rSjGST protein was induced to express by isopropy-β-D-thiogalactoside in prokaryotic hosts Escherichia coli with high efficiency and its antibodies were harvested.They can be applied to further study on immunodiagnostic methods of Schistosoma japonicum.
Objective: To isolate and purify recombinant Schistosoma japonicum (Chinese strain) protein glutathione S-transferase (rSjGST) for preparing the monoclonal antibodies (McAbs) against rSjGST.Methods: Balb/c mice were immunized with purified rSjGST protein.The anti-rSjGST monoclonal antibody was obtained through hybridoma technique.Characterization of the antibody was performed by ELISA and Western blot analysis.Results: The data had shown that high purified rSjGST protein and the hybridoma cell line 3C12 secreting McAbs against rSjGST protein were obtained.The subclass of McAbs was confirmed to be IgG1.Conclusions: The rSjGST protein was induced to express by isopropy-β-D-thiogalactoside in prokaryotic hosts Escherichia coli with high efficiency and its antibodies were harvested.They can be applied to further study on immunodiagnostic methods of Schistosoma japonicum.
2010, 35(12): 1200-1203.
Abstract:
Objective: To analyze the changes of microRNAs (miRNAs) expression in rat cardiac hypertrophy.Methods: To understand the roles of miRNAs in hypertrophy,a rat model of cardiac hypertrophy was established with abdominal aortic constriction (AAC).Real-time quantitative PCR was used to detect the changes of miRNAs expression in myocardium.Results: The heart weight/body weight,Nppa and myh7,the coding genes of atrial natriuretic peptide and β-myosin heavy chain (which were the markers of cardiac hypertrophy) were significant up-regulated on week 1 after AAC,it was meant the success of cardiac hypertrophy model.One week after aortic banding,miR-1,miR-133,miR-181a and miR-499 were significantly down-regulated,whereas miR-199a was up-regulated.Four weeks after AAC,miR-21,miR-24 and miR-214 were up-regulated,whereas miR-181a was significantly down-regulated,while miR-23a,miR-133,miR-145,miR-199a and miR-499 were unchanged.Conclusions: The expression of miRNAs was changed in rat cardiac hypertrophy,the changes of miRNAs maybe played an important role in regulation of cardiac hypertrophy pathological process.
Objective: To analyze the changes of microRNAs (miRNAs) expression in rat cardiac hypertrophy.Methods: To understand the roles of miRNAs in hypertrophy,a rat model of cardiac hypertrophy was established with abdominal aortic constriction (AAC).Real-time quantitative PCR was used to detect the changes of miRNAs expression in myocardium.Results: The heart weight/body weight,Nppa and myh7,the coding genes of atrial natriuretic peptide and β-myosin heavy chain (which were the markers of cardiac hypertrophy) were significant up-regulated on week 1 after AAC,it was meant the success of cardiac hypertrophy model.One week after aortic banding,miR-1,miR-133,miR-181a and miR-499 were significantly down-regulated,whereas miR-199a was up-regulated.Four weeks after AAC,miR-21,miR-24 and miR-214 were up-regulated,whereas miR-181a was significantly down-regulated,while miR-23a,miR-133,miR-145,miR-199a and miR-499 were unchanged.Conclusions: The expression of miRNAs was changed in rat cardiac hypertrophy,the changes of miRNAs maybe played an important role in regulation of cardiac hypertrophy pathological process.
2010, 35(12): 1204-1206.
Abstract:
Objective: To investigate the anti-aging effect and mechanism of dendrobium huoshenese capsule on drosophila.Methods: Newly trapped and not matea drosophila melangnogaster were collected within 8 hours,the drosophilas were cultured for 20 days in medium,and were randomly divided into 4 groups,each cultured with 0.000%,0.056%,0.167% and 0.500% concentrations of dendrobium huoshenese capsule.The drosophila's life span was detected with survival test.When the drosophilas were given varied concentrations of Dendrobium Huoshenese capsule for 10,20,30 days,superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured respectively.Results: When the drosophilas were treated with 0.056%,0.167%,0.500% concentrations of dendrobium huoshenese capsule,the half died time,maximum average life span and average life span were prolonged significantly (P<0.01),and SOD activity was increased remarkably (P<0.05) while MDA content was decreased (P<0.05) in the drosophila given the middle and high concentrations of dendrobium huoshenese capsule for 10,20,30 days.Conclusions: Dendrobium Huoshenese capsule has the anti-oxidation and anti-aging effects on drosophila.
Objective: To investigate the anti-aging effect and mechanism of dendrobium huoshenese capsule on drosophila.Methods: Newly trapped and not matea drosophila melangnogaster were collected within 8 hours,the drosophilas were cultured for 20 days in medium,and were randomly divided into 4 groups,each cultured with 0.000%,0.056%,0.167% and 0.500% concentrations of dendrobium huoshenese capsule.The drosophila's life span was detected with survival test.When the drosophilas were given varied concentrations of Dendrobium Huoshenese capsule for 10,20,30 days,superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured respectively.Results: When the drosophilas were treated with 0.056%,0.167%,0.500% concentrations of dendrobium huoshenese capsule,the half died time,maximum average life span and average life span were prolonged significantly (P<0.01),and SOD activity was increased remarkably (P<0.05) while MDA content was decreased (P<0.05) in the drosophila given the middle and high concentrations of dendrobium huoshenese capsule for 10,20,30 days.Conclusions: Dendrobium Huoshenese capsule has the anti-oxidation and anti-aging effects on drosophila.
2010, 35(12): 1207-1209,1213.
Abstract:
Objective: To observe the expression of CD34 in fetal scalp.Methods: The fetal scalp with follicles was from a fetus (at eight months of gestational age) within 2 h of his death after an abortion due to abnormal development.The tissue was divided into two copies.An equal part was prepared for the frozen sections labeled by CD34 immunofluorescence and observed by laser confocal microscope.Another part was digested by collagenase type Ⅱ for harvest of free hair follicles,which were furtherly treated by trypsin for cells suspension.The hair follicle cells were assayed by flow cytometry.Results: CD34 was mainly expressed in the outer root sheath bugle area of hair follicle under confocal microscopy,a minority was seen in the hair bulb and the basilar part of epidermis.The data from flow cytometry demonstrated that CD34 positive cells accounted for 8.54-13.04 percentages of total isolated follicle cells.Conclusions: Most CD34 positive cells are localized at the bulges of hair follicle which is presumed as the residence of follicle stem cells.The presence of CD34 positive cells in the follicular middle part and lower part around hair bulb,suggests the locations of stem cells and their immediately progenies varied along with the hair cycle.Furthermore,CD34 positive cells equably scatter at the basilar part of epidermis in the fatal scalp.
Objective: To observe the expression of CD34 in fetal scalp.Methods: The fetal scalp with follicles was from a fetus (at eight months of gestational age) within 2 h of his death after an abortion due to abnormal development.The tissue was divided into two copies.An equal part was prepared for the frozen sections labeled by CD34 immunofluorescence and observed by laser confocal microscope.Another part was digested by collagenase type Ⅱ for harvest of free hair follicles,which were furtherly treated by trypsin for cells suspension.The hair follicle cells were assayed by flow cytometry.Results: CD34 was mainly expressed in the outer root sheath bugle area of hair follicle under confocal microscopy,a minority was seen in the hair bulb and the basilar part of epidermis.The data from flow cytometry demonstrated that CD34 positive cells accounted for 8.54-13.04 percentages of total isolated follicle cells.Conclusions: Most CD34 positive cells are localized at the bulges of hair follicle which is presumed as the residence of follicle stem cells.The presence of CD34 positive cells in the follicular middle part and lower part around hair bulb,suggests the locations of stem cells and their immediately progenies varied along with the hair cycle.Furthermore,CD34 positive cells equably scatter at the basilar part of epidermis in the fatal scalp.
2010, 35(12): 1210-1213.
Abstract:
Objective: To investigate the immunogenity and immune regular ability of mesenchymal stem cells (MSCs) derived from rat bone marrow in mixed lymphocyte culture.Methods: MSCs and mixed lymphocyte reaction (MLR) cultures were set up.Splenic lymphocytes from SD rats were used as stimulator cells,and splenic lymphocytes from Wistar rat as responder cells.The study was composed of 6 groups.Group Ⅰ,the control group,was co-cultured with 1×105 stimulator cells and 1×105 responder cells,group Ⅱwith 1×104 MSCs from SD rats with the same amount of stimulator cells as group Ⅰ,group Ⅲ with 1×104 MSCs from SD rats and the same amount of stimulator and responder cells as group Ⅰ,group Ⅳ with 1×104 MSCs from SD rats and the same amount of stimulator and responder cells as group Ⅰ plus 1-methyltryptophan (1-MT),group V with 1×104 EPC from SD rats and the same amount of stimulator and responder cells as group Ⅰ plus phytohemagglutinin (PHA) as a mitogen,and group VI with 1×103 MSCs and the same amount of stimulator and responder cells as group Ⅰ.The culture time was 120 hours for each group.The number of lymphocyte in each group was calculated with a liquid scintillation counter.The MSCs and the content of tryptophane were determined by TRP-HPLC.Results: MSCs inhibited T-lymphocytes proliferation of MLR culture in a dose-dependent manner.The content of tryptophan decreased greatly in MSCs and MLR cultures.1-MT reversed the effect of MSCs.Conclusions: MSCs could suppress T lymphocyte responses via IDO enzyme activity.
Objective: To investigate the immunogenity and immune regular ability of mesenchymal stem cells (MSCs) derived from rat bone marrow in mixed lymphocyte culture.Methods: MSCs and mixed lymphocyte reaction (MLR) cultures were set up.Splenic lymphocytes from SD rats were used as stimulator cells,and splenic lymphocytes from Wistar rat as responder cells.The study was composed of 6 groups.Group Ⅰ,the control group,was co-cultured with 1×105 stimulator cells and 1×105 responder cells,group Ⅱwith 1×104 MSCs from SD rats with the same amount of stimulator cells as group Ⅰ,group Ⅲ with 1×104 MSCs from SD rats and the same amount of stimulator and responder cells as group Ⅰ,group Ⅳ with 1×104 MSCs from SD rats and the same amount of stimulator and responder cells as group Ⅰ plus 1-methyltryptophan (1-MT),group V with 1×104 EPC from SD rats and the same amount of stimulator and responder cells as group Ⅰ plus phytohemagglutinin (PHA) as a mitogen,and group VI with 1×103 MSCs and the same amount of stimulator and responder cells as group Ⅰ.The culture time was 120 hours for each group.The number of lymphocyte in each group was calculated with a liquid scintillation counter.The MSCs and the content of tryptophane were determined by TRP-HPLC.Results: MSCs inhibited T-lymphocytes proliferation of MLR culture in a dose-dependent manner.The content of tryptophan decreased greatly in MSCs and MLR cultures.1-MT reversed the effect of MSCs.Conclusions: MSCs could suppress T lymphocyte responses via IDO enzyme activity.
2010, 35(12): 1214-1216,1219.
Abstract:
Objective: To investigate the relationship between lymphatic microvessel density marked by monoclonal antibody D2-40 and Helicobacter pylori L-form (Hp-L) infection in gastric cancer.Methods: The expression of D2-40 and Hp-L in the tissue samples of 100 cases of gastric cancer,50 cases of peritumoral tissues and 30 normal gastric tissues were detect by S-P immunohistochemistry;and Hp-L infection of the above tissues was calculated by Gram stain.Results: The LMVD was 12.61±4.20 per 100 visual field in the central area of the tumor;and the LMVD was 31.20±3.73 per 100 visual field in the peritumoral tissues,which was significantly higher than that in the central area of the tumor and the normal gastric tissues 9.23±5.62 per 100 visual field (P<0.01);the number of LMVD was significantly correlated with that of metastatic lymph nodes and Hp-L infection (P<0.01).Conclusions: The lymphatic microvessel density marked by monoclonal antibody D2-40 is correlated with Hp-L infection.
Objective: To investigate the relationship between lymphatic microvessel density marked by monoclonal antibody D2-40 and Helicobacter pylori L-form (Hp-L) infection in gastric cancer.Methods: The expression of D2-40 and Hp-L in the tissue samples of 100 cases of gastric cancer,50 cases of peritumoral tissues and 30 normal gastric tissues were detect by S-P immunohistochemistry;and Hp-L infection of the above tissues was calculated by Gram stain.Results: The LMVD was 12.61±4.20 per 100 visual field in the central area of the tumor;and the LMVD was 31.20±3.73 per 100 visual field in the peritumoral tissues,which was significantly higher than that in the central area of the tumor and the normal gastric tissues 9.23±5.62 per 100 visual field (P<0.01);the number of LMVD was significantly correlated with that of metastatic lymph nodes and Hp-L infection (P<0.01).Conclusions: The lymphatic microvessel density marked by monoclonal antibody D2-40 is correlated with Hp-L infection.
2010, 35(12): 1217-1219.
Abstract:
Objective: To study the effect of different doses of manganese (Mn) on oxidative damage of liver mitochondrial induced by Mn chloride,and to explore the cell molecular mechanism of Mn-induced exeitotoxicity.Methods: Twenty four male SD rats were randomly divided into four groups by weight.The first group (control group) were given subcutaneous injection of physiological saline only;the second group (Mn-exposed group) were injected 32 mg/kg of MnCl2 subcutaneously;the third and fourth groups were intraperitoneally injected 100 mg/kg of taurine (Tau) and 1 mg/kg of cyclosporine A (CSA),respectively.Two hours after subcutaneous injection,the first group were intraperitoneally injected physiological saline,and the other three groups received intraperitoneal injection of 32 mg/kg of MnCl2 for 30 days,and Tau and CSA were injected every other day for 15 times.At the 24th hour after the last administration of MnCl2,the rats were killed and the liver was taken to detect the mitochondrial swelling amplitude,mitochondrial membrane potential (MMP),permeability transition pore (PT pore),the activity of GSH-PX,SDH and the content of cytochrome C and OH·.Results: Compared with control group,the mitochondrial swelling amplitude,PT pore,MMP,the activity of GSH-PX and SDH decreased in the MnCl2 group,while the content of cytochrome C and OH·increased (P<0.05 to P<0.01).Compared with MnCl2 group,the mitochondrial swelling amplitude,PT pore and MMP increased in Tau pretreatment group (P<0.05 to P<0.01),while the activity of GSH-PX and SDH increased,and the content of cytochrome C and OH·decreased (P<0.01).Compared with control group,the activity of GSH-PX and SDH,PT pore and MMP increased in CSA pretreatment group,while the content of cytochrome C decreased (P<0.01).Conclusions: Tau and CSA have some antagonistic effects on oxidative damage of liver mitochondrial induced by manganese.
Objective: To study the effect of different doses of manganese (Mn) on oxidative damage of liver mitochondrial induced by Mn chloride,and to explore the cell molecular mechanism of Mn-induced exeitotoxicity.Methods: Twenty four male SD rats were randomly divided into four groups by weight.The first group (control group) were given subcutaneous injection of physiological saline only;the second group (Mn-exposed group) were injected 32 mg/kg of MnCl2 subcutaneously;the third and fourth groups were intraperitoneally injected 100 mg/kg of taurine (Tau) and 1 mg/kg of cyclosporine A (CSA),respectively.Two hours after subcutaneous injection,the first group were intraperitoneally injected physiological saline,and the other three groups received intraperitoneal injection of 32 mg/kg of MnCl2 for 30 days,and Tau and CSA were injected every other day for 15 times.At the 24th hour after the last administration of MnCl2,the rats were killed and the liver was taken to detect the mitochondrial swelling amplitude,mitochondrial membrane potential (MMP),permeability transition pore (PT pore),the activity of GSH-PX,SDH and the content of cytochrome C and OH·.Results: Compared with control group,the mitochondrial swelling amplitude,PT pore,MMP,the activity of GSH-PX and SDH decreased in the MnCl2 group,while the content of cytochrome C and OH·increased (P<0.05 to P<0.01).Compared with MnCl2 group,the mitochondrial swelling amplitude,PT pore and MMP increased in Tau pretreatment group (P<0.05 to P<0.01),while the activity of GSH-PX and SDH increased,and the content of cytochrome C and OH·decreased (P<0.01).Compared with control group,the activity of GSH-PX and SDH,PT pore and MMP increased in CSA pretreatment group,while the content of cytochrome C decreased (P<0.01).Conclusions: Tau and CSA have some antagonistic effects on oxidative damage of liver mitochondrial induced by manganese.
2010, 35(12): 1220-1222.
Abstract:
Objective: To observe the relationship between BRAF V600E mutation in papillary thyroid carcinoma (PTC) and the sonographic findings.Methods: The study included 34 fresh PTC tissue samples and the neighboring tissues from the patients surgically confirmed PTC.The BRAF V600E mutation of all the cases was determined by allelespecific polymerase chain reaction and direct DNA sequencing.The ultrasound findings were compared between the nodules with and without mutation.Results: Of the 34 carcinoma samples,BRAF V600E mutation was observed in 18 cases and no BRAF V600E mutation in 16 cases.Of all the 34 neighboring samples,BRAF V600E mutation was observed in no one.The sonographic findings showed that patients with BRAF V600E mutation had no significant difference with those without BRAF V600E mutation in the tumor size,margin or calcification (P>0.05).Conclusions: BRAF V600E mutation is frequently observed in patients with PTC,and it has no significant connection with the sonographic findings of the nodules.Sonographic findings can't predict BRAF V600E mutation in the PTC.
Objective: To observe the relationship between BRAF V600E mutation in papillary thyroid carcinoma (PTC) and the sonographic findings.Methods: The study included 34 fresh PTC tissue samples and the neighboring tissues from the patients surgically confirmed PTC.The BRAF V600E mutation of all the cases was determined by allelespecific polymerase chain reaction and direct DNA sequencing.The ultrasound findings were compared between the nodules with and without mutation.Results: Of the 34 carcinoma samples,BRAF V600E mutation was observed in 18 cases and no BRAF V600E mutation in 16 cases.Of all the 34 neighboring samples,BRAF V600E mutation was observed in no one.The sonographic findings showed that patients with BRAF V600E mutation had no significant difference with those without BRAF V600E mutation in the tumor size,margin or calcification (P>0.05).Conclusions: BRAF V600E mutation is frequently observed in patients with PTC,and it has no significant connection with the sonographic findings of the nodules.Sonographic findings can't predict BRAF V600E mutation in the PTC.
2010, 35(12): 1223-1224,1227.
Abstract:
Objective: To investigate the effect of vinorelbine (NVB) plus cisplatin (DDP) regimen on recurrent metastatic breast cancer.Methods: Twenty-eight patients with taxane chemotherapy in patients with metastatic breast cancer were treated with NVB plus DDP program.NVB was 25 mg/m2,iv d1,d8;DDP was 25 mg/m2,iv d1-d3.The period was 21 days.The effect was evaluated after two periods.Results: In 28 patients,complete remission 2 cases,partial remission 11 cases,stable 10 cases,progress 5 cases,the total effective rate was 46.4%.One site metastasis rate was 50.0%,two or more than two parts metastasis rate was 43.8%.Conclusions: NVB plus DDP for treatment of taxane chemotherapy in recurrent metastatic breast cancer with failure plays a certain efficacy,and the toxicity can be tolerated.
Objective: To investigate the effect of vinorelbine (NVB) plus cisplatin (DDP) regimen on recurrent metastatic breast cancer.Methods: Twenty-eight patients with taxane chemotherapy in patients with metastatic breast cancer were treated with NVB plus DDP program.NVB was 25 mg/m2,iv d1,d8;DDP was 25 mg/m2,iv d1-d3.The period was 21 days.The effect was evaluated after two periods.Results: In 28 patients,complete remission 2 cases,partial remission 11 cases,stable 10 cases,progress 5 cases,the total effective rate was 46.4%.One site metastasis rate was 50.0%,two or more than two parts metastasis rate was 43.8%.Conclusions: NVB plus DDP for treatment of taxane chemotherapy in recurrent metastatic breast cancer with failure plays a certain efficacy,and the toxicity can be tolerated.
2010, 35(12): 1225-1227.
Abstract:
Objective: To investigate the effect of Nuss procedure with non-thoracoscope in treatment of funnel chest in children.Methods: Twenty-one children with funnel chest had Nuss operation with no assistance of thoracoscope.A small incision was made in each lateral chestwall,a steel bar was inserted under the sternum from the right thoracic cavity,and then the bar was turned over to raise the sternum to the normal position.The bar was fixed to the bilateral costal bones by using two stabilizing bars.Results: The operation was successful in all the cases.The operation time ranged from 30 to 50 min,and the average blood loss was less than 10 ml.The average hospital stay was 6.2 days,pneumothorax occurred in 4 patients after the operation.The results were excellent in 17 cases and good in 4 cases.All the patients were followed up for 1-20 months with no complaints.Conclusions: Modified Nuss procedure with no assistance of thoracoscope is easy to operate with minimal invasion and good short-term results.It is worth trying and popularizing although its long term result is yet to be observed.
Objective: To investigate the effect of Nuss procedure with non-thoracoscope in treatment of funnel chest in children.Methods: Twenty-one children with funnel chest had Nuss operation with no assistance of thoracoscope.A small incision was made in each lateral chestwall,a steel bar was inserted under the sternum from the right thoracic cavity,and then the bar was turned over to raise the sternum to the normal position.The bar was fixed to the bilateral costal bones by using two stabilizing bars.Results: The operation was successful in all the cases.The operation time ranged from 30 to 50 min,and the average blood loss was less than 10 ml.The average hospital stay was 6.2 days,pneumothorax occurred in 4 patients after the operation.The results were excellent in 17 cases and good in 4 cases.All the patients were followed up for 1-20 months with no complaints.Conclusions: Modified Nuss procedure with no assistance of thoracoscope is easy to operate with minimal invasion and good short-term results.It is worth trying and popularizing although its long term result is yet to be observed.
2010, 35(12): 1228-1230.
Abstract:
Objective: To understand the clinic pathological characteristics,the diagnosis and differential diagnosis of gangliocytic paraganglioma (GP).Methods: One case of urinary bladder GP was observed by light microscopy and immunohistochemistry,and studied combined with related literatures.Results: The tumor was composed of three type of cells:epithelioid cells,spindle Schwann's cell-like cells and mature ganglion cells.Immunohistochemically,the epithelioid cells were positive for CgA,Syn,NSE;the spindle cells were positive for Vim,NF,S-100 protein;and the ganglion cells were positive for GFAP,NSE,NF.Conclusions: GP is a rare benign tumor which commonly located in the duodenum,also can located in urinary bladder.The correct diagnosis can be decided accrording to its pathological characteristics and immunophenotype.
Objective: To understand the clinic pathological characteristics,the diagnosis and differential diagnosis of gangliocytic paraganglioma (GP).Methods: One case of urinary bladder GP was observed by light microscopy and immunohistochemistry,and studied combined with related literatures.Results: The tumor was composed of three type of cells:epithelioid cells,spindle Schwann's cell-like cells and mature ganglion cells.Immunohistochemically,the epithelioid cells were positive for CgA,Syn,NSE;the spindle cells were positive for Vim,NF,S-100 protein;and the ganglion cells were positive for GFAP,NSE,NF.Conclusions: GP is a rare benign tumor which commonly located in the duodenum,also can located in urinary bladder.The correct diagnosis can be decided accrording to its pathological characteristics and immunophenotype.
2010, 35(12): 1231-1232,1235.
Abstract:
Objective: To observe the effect of loop electrosurgical excision procedure (LEEP) in treatment of cervical intraepithelial neoplasia (CIN).Methods: The clinical data of 107 cases of CIN received LEEP were analyzed retrospectively.Results: Eighty-three cases demonstrated coincident pathological results before and after LEEP (77.57%);4 cases aggravated,one of which proved to be cervical cancer;the pathological changes in 20 cases were not obvious.The cure rates were 95.19% and 97.12% six months and two years respectively after LEEP operation.Conclusions: LEEP is a highly effective and safe procedure for diagnosis and treatment of CIN.
Objective: To observe the effect of loop electrosurgical excision procedure (LEEP) in treatment of cervical intraepithelial neoplasia (CIN).Methods: The clinical data of 107 cases of CIN received LEEP were analyzed retrospectively.Results: Eighty-three cases demonstrated coincident pathological results before and after LEEP (77.57%);4 cases aggravated,one of which proved to be cervical cancer;the pathological changes in 20 cases were not obvious.The cure rates were 95.19% and 97.12% six months and two years respectively after LEEP operation.Conclusions: LEEP is a highly effective and safe procedure for diagnosis and treatment of CIN.
2010, 35(12): 1233-1235.
Abstract:
Objective: To study the expression of cell division cycle 2 (CDC2) in breast carcinoma,and their associations with clinicopathological parameters and the relationship between CDC2 and C-erbB-2.Methods: Immunohistochemistry (EliVision) was used to detect the expression of CDC2 and C-erbB-2 in 60 cases of invasive breast carcinomas.Results: The positive expression of CDC2 in breast carcinoma was 61.7%.The expression of CDC2 was correlated with the histological grade (P<0.01),and there was a positive correlation between CDC2 and C-erbB-2 (P<0.01).Conclusions: High expression of CDC2 protein may play an important role in the pathogenesis and progression of breast carcinoma;CDC2 may advance the proliferation of cells.The expression of CDC2 and C-erbB-2 may be useful parameters in evaluating the biological behaviors of breast carcinoma.
Objective: To study the expression of cell division cycle 2 (CDC2) in breast carcinoma,and their associations with clinicopathological parameters and the relationship between CDC2 and C-erbB-2.Methods: Immunohistochemistry (EliVision) was used to detect the expression of CDC2 and C-erbB-2 in 60 cases of invasive breast carcinomas.Results: The positive expression of CDC2 in breast carcinoma was 61.7%.The expression of CDC2 was correlated with the histological grade (P<0.01),and there was a positive correlation between CDC2 and C-erbB-2 (P<0.01).Conclusions: High expression of CDC2 protein may play an important role in the pathogenesis and progression of breast carcinoma;CDC2 may advance the proliferation of cells.The expression of CDC2 and C-erbB-2 may be useful parameters in evaluating the biological behaviors of breast carcinoma.
2010, 35(12): 1236-1237.
Abstract:
Objective: To discuss the surgical procedures for congenital intestinal atresia,and the factors affecting the prognosis.Methods: The clinical data of 38 cases of congenital intestinal atresia from 1990 to 2009 were retrospectively analyzed.Results: Of the 38 cases of condenital intestinal atresia,5 had duodenor atresia,30 small intestine atresia and 3 colon atresia;7 cases were of septumtype atresia type,28 cases caecum atresia type and 3 cases multiplelevel atresia type.Two cases were complicated with malrotation of intestine and 3 cases with malrotation of intestine.Thirty-four cases were cured and survived,2 died and 2 gave up therapy.The curative rate was 89.47%.Conclusions: Surgical therapy is the only choice for treatment of congenital intestinal atresia.The procedure should be determined according to the position and type of the atresia.The effect and prognosis are influenced by multiple factors.
Objective: To discuss the surgical procedures for congenital intestinal atresia,and the factors affecting the prognosis.Methods: The clinical data of 38 cases of congenital intestinal atresia from 1990 to 2009 were retrospectively analyzed.Results: Of the 38 cases of condenital intestinal atresia,5 had duodenor atresia,30 small intestine atresia and 3 colon atresia;7 cases were of septumtype atresia type,28 cases caecum atresia type and 3 cases multiplelevel atresia type.Two cases were complicated with malrotation of intestine and 3 cases with malrotation of intestine.Thirty-four cases were cured and survived,2 died and 2 gave up therapy.The curative rate was 89.47%.Conclusions: Surgical therapy is the only choice for treatment of congenital intestinal atresia.The procedure should be determined according to the position and type of the atresia.The effect and prognosis are influenced by multiple factors.
2010, 35(12): 1238-1239,1242.
Abstract:
Objective: To study the changes of the expression of placental growth factor (PLGF) in placenta and soluble fms-like tyrosine kinase-1 (sFlt-1) in serum of patients with severe pre-eclampsia.Methods: Twenty normal pregnant women (control group) and 32 women with severe pre-eclampsia were selected.The expression level of PLGF in placenta among the two groups was determined by immunohistochemistry S-P mothod,and the results were analyzed with imagepro plus analysis software.The serum sFlt-1 level of the two groups was measured by ELISA.Results: The expression of PLGF in the trophoblast of patients with severe pre-eclampsia was lower than that in the control group (P<0.05),while the serum sFlt-1 level was significantly higher than that of the control group (P<0.01).Conclusions: The PLGF in placenta and sFlt-1 in serum may be related to the etiology of pre-eclampsia.The level of sFlt-1 in maternal serum may serve as a diagnostic marker of pre-eclampsia.
Objective: To study the changes of the expression of placental growth factor (PLGF) in placenta and soluble fms-like tyrosine kinase-1 (sFlt-1) in serum of patients with severe pre-eclampsia.Methods: Twenty normal pregnant women (control group) and 32 women with severe pre-eclampsia were selected.The expression level of PLGF in placenta among the two groups was determined by immunohistochemistry S-P mothod,and the results were analyzed with imagepro plus analysis software.The serum sFlt-1 level of the two groups was measured by ELISA.Results: The expression of PLGF in the trophoblast of patients with severe pre-eclampsia was lower than that in the control group (P<0.05),while the serum sFlt-1 level was significantly higher than that of the control group (P<0.01).Conclusions: The PLGF in placenta and sFlt-1 in serum may be related to the etiology of pre-eclampsia.The level of sFlt-1 in maternal serum may serve as a diagnostic marker of pre-eclampsia.
2010, 35(12): 1240-1242.
Abstract:
Objective: To explore the reduction,security,reliability and efficacy of anterior surgery on irreducible dislocation of the lower cervical spine.Methods: Fourteen cases of the lower cervical dislocation that could not be reduced by the skull traction received anterior surgery;nine cases of unilateral dislocation of the upper and lower vertebrae were distracted and rotated to untie the interlocking and then reduced;five cases of bilateral dislocation of the upper and lower vertebrae were distracted and depressed the upper vertebral body and pried the lower one to untie the interlocking and reduced,and then bone graft was performed and a steel plate was used for fixation.Results: The follow-up lasted from to 48 months.One case of C4 dislocation with paraplegia died one week after the operation because of respiratory muscle paralysis.The other 13 cases were evaluated according to ASIA Classification Standard:4 cases which had no response to the therapy were classified as grade A,4 cases were classified as grade B,of which 2 cases improved and were elevated to grade C,3 cases were rated as grade C and then improved to grade D,and 2 cases were rated as grade D and then improved to grade E.Conclusions: The anterior surgery can untie the interlocking and reach reduction at one stage,which may avoid complicated operations such as associated approach of anterior and posterior surgery or traditional surgery with the skull traction,thus reducing surgical trauma and risk.
Objective: To explore the reduction,security,reliability and efficacy of anterior surgery on irreducible dislocation of the lower cervical spine.Methods: Fourteen cases of the lower cervical dislocation that could not be reduced by the skull traction received anterior surgery;nine cases of unilateral dislocation of the upper and lower vertebrae were distracted and rotated to untie the interlocking and then reduced;five cases of bilateral dislocation of the upper and lower vertebrae were distracted and depressed the upper vertebral body and pried the lower one to untie the interlocking and reduced,and then bone graft was performed and a steel plate was used for fixation.Results: The follow-up lasted from to 48 months.One case of C4 dislocation with paraplegia died one week after the operation because of respiratory muscle paralysis.The other 13 cases were evaluated according to ASIA Classification Standard:4 cases which had no response to the therapy were classified as grade A,4 cases were classified as grade B,of which 2 cases improved and were elevated to grade C,3 cases were rated as grade C and then improved to grade D,and 2 cases were rated as grade D and then improved to grade E.Conclusions: The anterior surgery can untie the interlocking and reach reduction at one stage,which may avoid complicated operations such as associated approach of anterior and posterior surgery or traditional surgery with the skull traction,thus reducing surgical trauma and risk.
2010, 35(12): 1243-1244,1247.
Abstract:
Objective: To investigate the clinical value of laparoscopy-assisted vaginoplasty with free-lamellar peritoneum for patients with congenital absence of vagina.Methods: The clinical data of 18 cases with congenital absence of vagina received laparoscopy-assisted vaginoplasty with free-lamellar peritoneum were reviewed,and the operation outcome was analyzed.Results: The operation was successful in all the 18 cases,and no complications occurred.All the cases were followed up for 3 to 12 months.The artificial vaginas were found to be in good growth,and the vaginal wall was pale red,soft and moist with good elasticity.All the cases had a normal vaginal appearance and satisfactory sexual life.Conclusions: Laparoscopy-assisted vaginoplasty with free-lamellar peritoneum is safe and has the advantages of minimal invasion,rapid recovery and good therapeutic efficacy.It is an ideal modus operandi for patients with congenital absence of vagina.
Objective: To investigate the clinical value of laparoscopy-assisted vaginoplasty with free-lamellar peritoneum for patients with congenital absence of vagina.Methods: The clinical data of 18 cases with congenital absence of vagina received laparoscopy-assisted vaginoplasty with free-lamellar peritoneum were reviewed,and the operation outcome was analyzed.Results: The operation was successful in all the 18 cases,and no complications occurred.All the cases were followed up for 3 to 12 months.The artificial vaginas were found to be in good growth,and the vaginal wall was pale red,soft and moist with good elasticity.All the cases had a normal vaginal appearance and satisfactory sexual life.Conclusions: Laparoscopy-assisted vaginoplasty with free-lamellar peritoneum is safe and has the advantages of minimal invasion,rapid recovery and good therapeutic efficacy.It is an ideal modus operandi for patients with congenital absence of vagina.
2010, 35(12): 1245-1247.
Abstract:
Objective: To assess the efficacy and safety of demostic product levobupivacaine for hysterectomy under combined spinal-epidural anesthesia (CSEA).Methods: One hundred patients ASA gradeⅠorⅡundergoing elective hysterectomy under CSEA were randomly divided into 2 groups (n=50 each):levobupivacaine and ropivacaine.Each group was received intrathecal 0.75% levobupivacaine or ropivacaine injection 2 ml.The onset time and duration of sensory and motor block,the block height,degree of abdominal muscle relaxation,anesthetic efficacy and side effects were recorded.Results: There was no significant difference in the onset time,duration and degree of sensory and motor block among the 2 groups (P>0.05).There was no significant difference including mean arterial blood pressure and the degree of abdominal muscle relaxation and anesthetic efficacy and side effects.While vital signs of heart rate and arterial oxygen saturation had significant differences after anesthesia (P<0.01).Conclusions: The demostic product levobupivacaine for hysterectomy has satisfactory anesthetic effects compared with ropivacaine under CSEA and has no significant side effects.
Objective: To assess the efficacy and safety of demostic product levobupivacaine for hysterectomy under combined spinal-epidural anesthesia (CSEA).Methods: One hundred patients ASA gradeⅠorⅡundergoing elective hysterectomy under CSEA were randomly divided into 2 groups (n=50 each):levobupivacaine and ropivacaine.Each group was received intrathecal 0.75% levobupivacaine or ropivacaine injection 2 ml.The onset time and duration of sensory and motor block,the block height,degree of abdominal muscle relaxation,anesthetic efficacy and side effects were recorded.Results: There was no significant difference in the onset time,duration and degree of sensory and motor block among the 2 groups (P>0.05).There was no significant difference including mean arterial blood pressure and the degree of abdominal muscle relaxation and anesthetic efficacy and side effects.While vital signs of heart rate and arterial oxygen saturation had significant differences after anesthesia (P<0.01).Conclusions: The demostic product levobupivacaine for hysterectomy has satisfactory anesthetic effects compared with ropivacaine under CSEA and has no significant side effects.
2010, 35(12): 1248-1249.
Abstract:
Objective: To evaluate the clinical diagnosis and treatment of traumatic cerebral infarction.Methods: A combination use of drugs and proper surgical procedure of 28 cases of traumatic cerebral infarction were analyzed.Results: One year after injury,the outcome of the patients was evaluated according to Glasgow outcome scale:one point (death) was observed in 5 cases,2 points (plant life) in 4 cases,3 points (severe disability) in 9 cases,4 points (moderate disability) in 5 cases and 5 points (good recovery) in 5 cases.Conclusions: Dynamic CT or MRI examination may help to make early diagnosis of traumatic cerebral infarction.A combination use of drugs and proper surgical procedure are the main methods for treatment of traumatic cerebral infarction.
Objective: To evaluate the clinical diagnosis and treatment of traumatic cerebral infarction.Methods: A combination use of drugs and proper surgical procedure of 28 cases of traumatic cerebral infarction were analyzed.Results: One year after injury,the outcome of the patients was evaluated according to Glasgow outcome scale:one point (death) was observed in 5 cases,2 points (plant life) in 4 cases,3 points (severe disability) in 9 cases,4 points (moderate disability) in 5 cases and 5 points (good recovery) in 5 cases.Conclusions: Dynamic CT or MRI examination may help to make early diagnosis of traumatic cerebral infarction.A combination use of drugs and proper surgical procedure are the main methods for treatment of traumatic cerebral infarction.
2010, 35(12): 1250-1251,1254.
Abstract:
Objective: To study the diagnosis and treatment of spontaneous rupture of renal hamartoma.Methods: Ten cases of renal hamartoma rupture were received B ultrasound and CT scan,and 7 of them received contrast enhancement computerized tomography.All the 10 cases were performed nephrectomy.Results: All the cases were recovered well after operation.Nine cases were confirmed to be renal hamartoma by pathology after operation and 1 case proved to be renal hamartoma with partial canceration.Conclusions: B ultrasound and CT scan are effective and simple means to make final diagnosis of renal hamartoma.The main therapy is nephrectomy,and sometimes nephron-sparing surgery can be adopted.
Objective: To study the diagnosis and treatment of spontaneous rupture of renal hamartoma.Methods: Ten cases of renal hamartoma rupture were received B ultrasound and CT scan,and 7 of them received contrast enhancement computerized tomography.All the 10 cases were performed nephrectomy.Results: All the cases were recovered well after operation.Nine cases were confirmed to be renal hamartoma by pathology after operation and 1 case proved to be renal hamartoma with partial canceration.Conclusions: B ultrasound and CT scan are effective and simple means to make final diagnosis of renal hamartoma.The main therapy is nephrectomy,and sometimes nephron-sparing surgery can be adopted.
2010, 35(12): 1252-1254.
Abstract:
Objective: To discuss the role of tracheal stent in benign tracheal stenosis.Methods: Eight cases of benign tracheal stenosis were implanted the tracheal stent with the guidance of accurate digital substrution angiography positioning.Results: All stents were implanted successfully for one time.There were altogether 9 stents implanted,including 5 tracheal stents,2 tracheal-bronchial stents and 2 bronchial stents.The symptoms of dyspnea and respiratory rapidity were quickly relieved after treatment.Conclusions: Placement of tracheal stent is safe and effective for treatment of benign tracheal stenosis.
Objective: To discuss the role of tracheal stent in benign tracheal stenosis.Methods: Eight cases of benign tracheal stenosis were implanted the tracheal stent with the guidance of accurate digital substrution angiography positioning.Results: All stents were implanted successfully for one time.There were altogether 9 stents implanted,including 5 tracheal stents,2 tracheal-bronchial stents and 2 bronchial stents.The symptoms of dyspnea and respiratory rapidity were quickly relieved after treatment.Conclusions: Placement of tracheal stent is safe and effective for treatment of benign tracheal stenosis.
2010, 35(12): 1255-1256,1259.
Abstract:
Objective: To explore the clinical diagnosis and treatment of progressive hemorrhage injury (PHI) due to craniocerebral trauma.Methods: The clinical data of 46 patients with PHI were analyzed retrospectively,and the clinical features were discussed according to the Glasgow coma score at admission,first CT time after injury,hematoma type and PHI distribution.Results: The PHI was most often detected in patients with severe primary brain injury or receiving early CT rescue after injury;among the 46 PHI cases,25 had intracerebral hematoma,11 epidural hematoma,7 subdural hematoma and 3 subarachnoid hemorrhages.Satisfactory results were observed in 20 cases,slight disability in 12,sever disability in 8 and mortality in 6.Conclusions: Patients with severe traumatic brain injury,especially those receiving CT examination within 2 hours after injury should be rechecked within 6 hours to detect PHI.Early diagnosis and treatment may help to improve the patients' cure rate and reduce the mortality and rate of disability.
Objective: To explore the clinical diagnosis and treatment of progressive hemorrhage injury (PHI) due to craniocerebral trauma.Methods: The clinical data of 46 patients with PHI were analyzed retrospectively,and the clinical features were discussed according to the Glasgow coma score at admission,first CT time after injury,hematoma type and PHI distribution.Results: The PHI was most often detected in patients with severe primary brain injury or receiving early CT rescue after injury;among the 46 PHI cases,25 had intracerebral hematoma,11 epidural hematoma,7 subdural hematoma and 3 subarachnoid hemorrhages.Satisfactory results were observed in 20 cases,slight disability in 12,sever disability in 8 and mortality in 6.Conclusions: Patients with severe traumatic brain injury,especially those receiving CT examination within 2 hours after injury should be rechecked within 6 hours to detect PHI.Early diagnosis and treatment may help to improve the patients' cure rate and reduce the mortality and rate of disability.
2010, 35(12): 1257-1259.
Abstract:
Objective: To investigate the clinical outcome and indication of one stage combined anterior-posterior decompression for severe cervical spondylotic myelopathy (CSM).Methods: Retrospective analysis of 12 cases with severe CSM were performed one stage combined anterior-posterior decompression.Japanese Orthopedic Association (JOA) score system and the clinical outcome were used to evaluate the neural function.The improvement rate and clinical curative effect were evaluated.Results: The surgical time was 2.5-5 hours,blood loss was 480-1 200 ml.No severe intraoperative complication was noted except cerebrospinal fluid leakage in 2 cases.All cases were followed up from 6 months to 4 years,both anterior grafts and posterior door axial had bony union.All cases had excellent neural function improve rate.MRI showed dural cyst moved dorsally and decompression in all cases,CT showed the spinal canal was enlarged sufficiently and the average angle of open-door was 45.2°.Conclusions: When patients with complex multi segmental CSM are carefully selected and surgery is indicated,the one-stage combined anterior-posterior decompression approach can achieve good short-term clinical effect.
Objective: To investigate the clinical outcome and indication of one stage combined anterior-posterior decompression for severe cervical spondylotic myelopathy (CSM).Methods: Retrospective analysis of 12 cases with severe CSM were performed one stage combined anterior-posterior decompression.Japanese Orthopedic Association (JOA) score system and the clinical outcome were used to evaluate the neural function.The improvement rate and clinical curative effect were evaluated.Results: The surgical time was 2.5-5 hours,blood loss was 480-1 200 ml.No severe intraoperative complication was noted except cerebrospinal fluid leakage in 2 cases.All cases were followed up from 6 months to 4 years,both anterior grafts and posterior door axial had bony union.All cases had excellent neural function improve rate.MRI showed dural cyst moved dorsally and decompression in all cases,CT showed the spinal canal was enlarged sufficiently and the average angle of open-door was 45.2°.Conclusions: When patients with complex multi segmental CSM are carefully selected and surgery is indicated,the one-stage combined anterior-posterior decompression approach can achieve good short-term clinical effect.
2010, 35(12): 1260-1262.
Abstract:
Objective: To evaluate continuous electroencephalography (CEEG) monitoring in assessing and predicting the prognosis of patients with critical cerebral hemorrhage.Methods: CEEG's classification was dynamically recorded at the bedside of 30 cases of critical cerebral hemorrhage with Glasgow coma scale ≤ 8.The outcome was evaluated cording to the Young criteria.All the patients were followed up for 2 months,and the correlation between the results of CEEG's monitoring and the prognosis of the patients was analyzed.Results: The CEEG of 30 patients with critical cerebral hemorrhage was nearly always markedly abnormal.The occurrence of nonconvulsive seizures was observed in 10% of the patients and convulsive seizures in 3.3%.The unfavorable prognosis rate in the cases with CEEG classification of Ⅰ and Ⅱ was 11.8%,while it was 53.8% in cases with CEEG classification of Ⅲ-Ⅵ.The difference was significant (P=0.019).Conclusions: The CEEG classification is markedly correlated with the prognosis of patients with acute critical cerebral hemorrhage,and CEEG monitoring might be of certain value in predicting the outcome.
Objective: To evaluate continuous electroencephalography (CEEG) monitoring in assessing and predicting the prognosis of patients with critical cerebral hemorrhage.Methods: CEEG's classification was dynamically recorded at the bedside of 30 cases of critical cerebral hemorrhage with Glasgow coma scale ≤ 8.The outcome was evaluated cording to the Young criteria.All the patients were followed up for 2 months,and the correlation between the results of CEEG's monitoring and the prognosis of the patients was analyzed.Results: The CEEG of 30 patients with critical cerebral hemorrhage was nearly always markedly abnormal.The occurrence of nonconvulsive seizures was observed in 10% of the patients and convulsive seizures in 3.3%.The unfavorable prognosis rate in the cases with CEEG classification of Ⅰ and Ⅱ was 11.8%,while it was 53.8% in cases with CEEG classification of Ⅲ-Ⅵ.The difference was significant (P=0.019).Conclusions: The CEEG classification is markedly correlated with the prognosis of patients with acute critical cerebral hemorrhage,and CEEG monitoring might be of certain value in predicting the outcome.
2010, 35(12): 1262-1264.
Abstract:
Objective: To investigate the therapeutic effect of noninvasive bi-level positive airway pressure (BiPAP) ventilation combined with respiratory stimulant treatment of patients with chronic obstructive pulmonary disease (COPD) acute aggravation complicated with pulmonary encephalopathy.Methods: Eighty patients with COPD complicated with pulmonary encephalopathy were divided into BiPAP group simply and BiPAP combined with repiratory stimulant group randomly.The general condition and changes of blood gas analysis in the two groups were observed.Results: Compared with BiPAP group,patients in combination treatment group showed more rapid recovery,these parameters including breathing,heart rate,arterial blood pH,PaO2 and PaCO2 improvements were better than in simple ventilation group (P<0.05~P<0.01).The efficacy rate in the two groups was no statistical difference (P>0.05).Conclusions: In COPD patients complicated with pulmonary encephalopathy,BiPAP combined with respiratory stimulant is an effective and noninvasive method which can reduce some side effects such as tracheal intubation or tracheotomy.
Objective: To investigate the therapeutic effect of noninvasive bi-level positive airway pressure (BiPAP) ventilation combined with respiratory stimulant treatment of patients with chronic obstructive pulmonary disease (COPD) acute aggravation complicated with pulmonary encephalopathy.Methods: Eighty patients with COPD complicated with pulmonary encephalopathy were divided into BiPAP group simply and BiPAP combined with repiratory stimulant group randomly.The general condition and changes of blood gas analysis in the two groups were observed.Results: Compared with BiPAP group,patients in combination treatment group showed more rapid recovery,these parameters including breathing,heart rate,arterial blood pH,PaO2 and PaCO2 improvements were better than in simple ventilation group (P<0.05~P<0.01).The efficacy rate in the two groups was no statistical difference (P>0.05).Conclusions: In COPD patients complicated with pulmonary encephalopathy,BiPAP combined with respiratory stimulant is an effective and noninvasive method which can reduce some side effects such as tracheal intubation or tracheotomy.
2010, 35(12): 1265-1266.
Abstract:
Objective: To investigate the clinical effect of radiofrequency ablation in treating dysfunctional uterine bleeding.Methods: Thirty cases of dysfunctional uterine bleeding were treated by radiofrequency ablation in the guidance of B-type ultrasonic and followed for 6 months.Results: There were 18 cases of amenorrhea,5 cases of lower menstrual blood volume and 6 cases of scanty menses in the 30 cases.The total effective rate was 96.67% in this study.Conclusions: The radiofrequency ablation for treating dysfunctional uterine bleeding is proved to be a safe,simple,convenient and effective minor invasiveness operation method.
Objective: To investigate the clinical effect of radiofrequency ablation in treating dysfunctional uterine bleeding.Methods: Thirty cases of dysfunctional uterine bleeding were treated by radiofrequency ablation in the guidance of B-type ultrasonic and followed for 6 months.Results: There were 18 cases of amenorrhea,5 cases of lower menstrual blood volume and 6 cases of scanty menses in the 30 cases.The total effective rate was 96.67% in this study.Conclusions: The radiofrequency ablation for treating dysfunctional uterine bleeding is proved to be a safe,simple,convenient and effective minor invasiveness operation method.
2010, 35(12): 1267-1269.
Abstract:
Objective: To investigate the protective mechanism and efficacy of Xuebijing injection on multiple organ functions in critical ill patients.Methods: Fifty-two critical ill patients were randomly divided into Xuebijing group and contrast group.In Xuebijing group,50 ml Xuebijing injection was dropped intravenously twice per day and continued for 10 days.The other treatment given to two groups were same,including anti-inflammation,symptomatic treatment,nutritional support therapy,doing operation for trauma patients,applying auxiliary breath for some patients according to patient's condition and so on.The levels of some inflammatory mediators including tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) and IL-8 and serum biochemical indexes were determined on the first,4th and 10th day after treatment.Results: After treatment,the levels of TNF-α,IL-6 and IL-8 were significantly decreased in these two groups,and it was more obvious in Xuebijing group compared with contrast group (P<0.01);the levels of serum alanine aminotransferase,aspartate aminotransferase,creatinine,blood urea nitrogen,actate dehydrogenase,creatine kinase and isoenzyme of creatine kinase were significantly decreased after treatment in these two groups (P<0.01),and all these indexes were decreased more remarkably in Xuebijing group (P<0.01).Conclusions: Xuebijing injection has an important protection effect on liver,renal and heart in critical ill patients.
Objective: To investigate the protective mechanism and efficacy of Xuebijing injection on multiple organ functions in critical ill patients.Methods: Fifty-two critical ill patients were randomly divided into Xuebijing group and contrast group.In Xuebijing group,50 ml Xuebijing injection was dropped intravenously twice per day and continued for 10 days.The other treatment given to two groups were same,including anti-inflammation,symptomatic treatment,nutritional support therapy,doing operation for trauma patients,applying auxiliary breath for some patients according to patient's condition and so on.The levels of some inflammatory mediators including tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) and IL-8 and serum biochemical indexes were determined on the first,4th and 10th day after treatment.Results: After treatment,the levels of TNF-α,IL-6 and IL-8 were significantly decreased in these two groups,and it was more obvious in Xuebijing group compared with contrast group (P<0.01);the levels of serum alanine aminotransferase,aspartate aminotransferase,creatinine,blood urea nitrogen,actate dehydrogenase,creatine kinase and isoenzyme of creatine kinase were significantly decreased after treatment in these two groups (P<0.01),and all these indexes were decreased more remarkably in Xuebijing group (P<0.01).Conclusions: Xuebijing injection has an important protection effect on liver,renal and heart in critical ill patients.
2010, 35(12): 1270-1271.
Abstract:
Objective: To observe the efficacy of early interference treatment for hand-foot-and-mouth disease with small doses methylprednisolone.Methods: One thousand eight hundred hand-foot-and-mouth disease patients were randomly divided into two groups.The treatment group (n=950) was treated with symptomatic treatment and intravenous drip treatment with methylprednisolone 1-2 mg·kg-1·d-1,the control group (n=850) was treated with symptomatic treatment and conventional treatment for abatement of fever.To investigate the therapeutic effect of the two groups of clinical treatment.Results: The treatment efficacy in the treatment group was higher than in the control group (P<0.01),comparing the time to allaying a fever,stopping body shaking,stopping frightened during sleep in two groups.Conclusions: The therapeutic effect in the treatment group with small doses of methylprednisolone early interference treatment was superiorer than in the control group with conventional symptomatic treatment,it was well worth clinical popularizing and application.
Objective: To observe the efficacy of early interference treatment for hand-foot-and-mouth disease with small doses methylprednisolone.Methods: One thousand eight hundred hand-foot-and-mouth disease patients were randomly divided into two groups.The treatment group (n=950) was treated with symptomatic treatment and intravenous drip treatment with methylprednisolone 1-2 mg·kg-1·d-1,the control group (n=850) was treated with symptomatic treatment and conventional treatment for abatement of fever.To investigate the therapeutic effect of the two groups of clinical treatment.Results: The treatment efficacy in the treatment group was higher than in the control group (P<0.01),comparing the time to allaying a fever,stopping body shaking,stopping frightened during sleep in two groups.Conclusions: The therapeutic effect in the treatment group with small doses of methylprednisolone early interference treatment was superiorer than in the control group with conventional symptomatic treatment,it was well worth clinical popularizing and application.
2010, 35(12): 1272-1274.
Abstract:
Objective: To investigate the effects of interleukin-10 (IL-10),tumor necrosis factor-α (TNF-α),and transforming growth factor-β (TGF-β) in pathogenesis and aggravating phase of chronic obstructive pulmonary disease (COPD).Methods: The serum contents of IL-10,TNF-α and TGF-β in acute exacerbation,catabasis phases of COPD and health control group were determeined by enzyme linked immunosorbent assay.Results: The levels of serum IL-10 in acute exacerbation and catabasis phases of COPD were lower than in normal control group (P<0.01);the levels of serum TNF-α of in acute exacerbation and catabasis phases of COPD were significantly higher than in normal control group (P<0.01);the content of serum TGF-β in acute exacerbation phase of COPD was higher than in normal control group and COPD catabasis group,but there was no significant difference between COPD catabasis phase and normal control group (P>0.05).TGF-β was positively correlated with IL-10 in COPD acute exacerbation phase.Conclusions: IL-10,TNF-α and TGF-β are all involved participate in the procedure of COPD pathogenesis and aggravating phase.The content of serum IL-10 in COPD is depressed,which maybe plays an important role in inflammatory reaction and aggravating phase of COPD.
Objective: To investigate the effects of interleukin-10 (IL-10),tumor necrosis factor-α (TNF-α),and transforming growth factor-β (TGF-β) in pathogenesis and aggravating phase of chronic obstructive pulmonary disease (COPD).Methods: The serum contents of IL-10,TNF-α and TGF-β in acute exacerbation,catabasis phases of COPD and health control group were determeined by enzyme linked immunosorbent assay.Results: The levels of serum IL-10 in acute exacerbation and catabasis phases of COPD were lower than in normal control group (P<0.01);the levels of serum TNF-α of in acute exacerbation and catabasis phases of COPD were significantly higher than in normal control group (P<0.01);the content of serum TGF-β in acute exacerbation phase of COPD was higher than in normal control group and COPD catabasis group,but there was no significant difference between COPD catabasis phase and normal control group (P>0.05).TGF-β was positively correlated with IL-10 in COPD acute exacerbation phase.Conclusions: IL-10,TNF-α and TGF-β are all involved participate in the procedure of COPD pathogenesis and aggravating phase.The content of serum IL-10 in COPD is depressed,which maybe plays an important role in inflammatory reaction and aggravating phase of COPD.
2010, 35(12): 1284-1286.
Abstract:
Objective: To analyze the important mammographic features of full-field digital mammography in infiltrative ductal carcinoma and raise the preoperative diagnosis level.Methods: Eighty-two cases confirmed by surgery and pathology were collected and retrospectively analysis in the study.All cases had been examined with full-field digital mammography before surgery.Results: Among 82 cases X-ray signs,62 cases were with round and irregular mass,48 cases with malignant calcification,which include 32 cases combining with masses,17 cases with local dissymmetrical infiltration or abnormal structures,9 cases with nipple retraction and 9 cases with local skin thickening,5 cases local skin retraction,3 cases with positive duct sign.Conclusions: Mass and malignant calcification are the most common features of infiltrative ductal carcinoma,which is important in diagnosis,and there are some unusal features,such as local dissymmetrical infiltration or abnormal structures,and the change of nipple and skin,and positive duct sign.
Objective: To analyze the important mammographic features of full-field digital mammography in infiltrative ductal carcinoma and raise the preoperative diagnosis level.Methods: Eighty-two cases confirmed by surgery and pathology were collected and retrospectively analysis in the study.All cases had been examined with full-field digital mammography before surgery.Results: Among 82 cases X-ray signs,62 cases were with round and irregular mass,48 cases with malignant calcification,which include 32 cases combining with masses,17 cases with local dissymmetrical infiltration or abnormal structures,9 cases with nipple retraction and 9 cases with local skin thickening,5 cases local skin retraction,3 cases with positive duct sign.Conclusions: Mass and malignant calcification are the most common features of infiltrative ductal carcinoma,which is important in diagnosis,and there are some unusal features,such as local dissymmetrical infiltration or abnormal structures,and the change of nipple and skin,and positive duct sign.
2010, 35(12): 1287-1288.
Abstract:
Objective: To study the value of two-dimensional and color Doppler ultrasound in the diagnosis of thyroid cancer.Methods: A retrospective analysis of 67 cases (a total of 72 nodules) of thyroid cancer was summarized,including their ultrasonographic images,border,capsule,internal echo,calcification,the characteristics of blood flows within and around the tumors and lymph node metastasis.Results: In 67 cases (72 nodules),66 cases with internal echo were showed with substantial echo (including 59 low-echo,6 iso-echo,1 high-echo),6 mixed echo;24 sand-like calcified nodules;nodular irregular in shape,the border was not clear;30 with incomplete capsule;65 with nodular peripheral and internal flow signals;29 with lymph node metastasis.Conclusions: Sonographic features of thyroid cancer has a high specificity,ultrasonography has a great value on early diagnosis of thyroid cancer,and it can provide an important clinical basis for surgical treatment,and it is useful for prognosis and following up.
Objective: To study the value of two-dimensional and color Doppler ultrasound in the diagnosis of thyroid cancer.Methods: A retrospective analysis of 67 cases (a total of 72 nodules) of thyroid cancer was summarized,including their ultrasonographic images,border,capsule,internal echo,calcification,the characteristics of blood flows within and around the tumors and lymph node metastasis.Results: In 67 cases (72 nodules),66 cases with internal echo were showed with substantial echo (including 59 low-echo,6 iso-echo,1 high-echo),6 mixed echo;24 sand-like calcified nodules;nodular irregular in shape,the border was not clear;30 with incomplete capsule;65 with nodular peripheral and internal flow signals;29 with lymph node metastasis.Conclusions: Sonographic features of thyroid cancer has a high specificity,ultrasonography has a great value on early diagnosis of thyroid cancer,and it can provide an important clinical basis for surgical treatment,and it is useful for prognosis and following up.
2010, 35(12): 1289-1290,1292.
Abstract:
Objective: To evaluate the diagnostic value of color Doppler uhrasonography (CDUS) in nutcracker syndrome by receiver operating characteristic curve (ROC curve).Methods: The left renal vein (LRV) was measured on horizontal decubitus in 43 patients and 23 control subjects by CDUS.The interior diameter (D1) and flow velocit (V1) of LRV between superior mesenteric artery (SMA) and abdominal artery (AA),the interior diameter (D2) and flow velocit (V2) of LRV near the left of AA were recorded and analyzed in order to diferentiate nutcracker syndrome and controls.Repeated measuring the parameters above after 15 minutes keeping back after the extension bit.ROC curve of CDUS diagnosing nutcracker syndrome were drawn.The areas under curves (AUC) and diagnosis cut-off points of parameters in nutcracker syndrome were calculated.Results: D2/D1 and V1/V2 in patients with nutcracker syndrome were higher significantly than those in normal control subjects (P<0.05),the AUC of D2/D1 and V1/V2 were 0.966 and 0.969,respectively.The diagnosis cut-off point of D2/D1 and V1/V2 was 3.1 and 4.3,respectively.Conclusions: CDUS is an effective diagnosis method for nutcracker syndrome.
Objective: To evaluate the diagnostic value of color Doppler uhrasonography (CDUS) in nutcracker syndrome by receiver operating characteristic curve (ROC curve).Methods: The left renal vein (LRV) was measured on horizontal decubitus in 43 patients and 23 control subjects by CDUS.The interior diameter (D1) and flow velocit (V1) of LRV between superior mesenteric artery (SMA) and abdominal artery (AA),the interior diameter (D2) and flow velocit (V2) of LRV near the left of AA were recorded and analyzed in order to diferentiate nutcracker syndrome and controls.Repeated measuring the parameters above after 15 minutes keeping back after the extension bit.ROC curve of CDUS diagnosing nutcracker syndrome were drawn.The areas under curves (AUC) and diagnosis cut-off points of parameters in nutcracker syndrome were calculated.Results: D2/D1 and V1/V2 in patients with nutcracker syndrome were higher significantly than those in normal control subjects (P<0.05),the AUC of D2/D1 and V1/V2 were 0.966 and 0.969,respectively.The diagnosis cut-off point of D2/D1 and V1/V2 was 3.1 and 4.3,respectively.Conclusions: CDUS is an effective diagnosis method for nutcracker syndrome.
2010, 35(12): 1291-1292.
Abstract:
Objective: To evaluate the clinical application value of percutaneous lung biopsy by improved CT fluoroscopy.Methods: Forty-four cases were analyzed percutaneous lung biopsy by improved CT fluoroscopy which were proved by operation or pathology with the puncture needle.The main points are:choose the best layer,point,angle,depth,draw and obtaining at many points.Results: All 44 lung lesions were successfully conducted biopsy through CT-guided fine needle aspiration,and the pathological confirmed diagnosis rate was 98%,pathological diagnosis 31 malignant tumors and 13 benign tumors.Conclusions: The pereutaneous lung biopsy by improved CT fluoroscopy is accurate,less of complication.It is a safe,effective,simple and practical way for diagnosing lung diseases.
Objective: To evaluate the clinical application value of percutaneous lung biopsy by improved CT fluoroscopy.Methods: Forty-four cases were analyzed percutaneous lung biopsy by improved CT fluoroscopy which were proved by operation or pathology with the puncture needle.The main points are:choose the best layer,point,angle,depth,draw and obtaining at many points.Results: All 44 lung lesions were successfully conducted biopsy through CT-guided fine needle aspiration,and the pathological confirmed diagnosis rate was 98%,pathological diagnosis 31 malignant tumors and 13 benign tumors.Conclusions: The pereutaneous lung biopsy by improved CT fluoroscopy is accurate,less of complication.It is a safe,effective,simple and practical way for diagnosing lung diseases.
2010, 35(12): 1293-1295.
Abstract:
Objective: To study the expression and the clinical significance of CD28 on CD8+ T lymphocytes in liver tissue of hepatitis B patients.Methods: One hundred cases of hepatitis B patients were divided into mild,moderate and severe hepatitis B groups according to the pathological changes of liver tissues,and normal liver tissue of 15 healthy persons as the control group.CD8+CD28+ and CD8+CD28-T lymphocytes were determined in liver tissue with immunohistochemical double-labeling.Results: The CD8+CD28+ and CD8+CD28-T lymphocytes in hepatitis B patients were significantly higher than those in control group (P<0.05).In hepatitis B patients with mild lesions,CD8+CD28+ T lymphocytes were decreased significantly compared with control group,in hepatitis B patients with moderate and severe lesions,CD8+CD28+ cells and CD8+CD28-T lymphocytes were increased significantly compared with control group (P<0.01).CD8+CD28+ T lymphocytes were raised with the liver inflammation grades,but CD8+CD28-T lymphocytes were reduced gradually with the liver inflammation grades.Conclusions: The expression of CD28 on the surface of CD8+ T lymphocytes in liver tissue of patients with hepatitis B is closely related to liver tissue lesion.The CD8+CD28+ T lymphocytes in liver tissue can be used as immunological markers of liver injury in patients with hepatitis B.CD8+CD28-T lymphocytes in liver tissue may be the main reason for persistent infection of hepatitis B virus.
Objective: To study the expression and the clinical significance of CD28 on CD8+ T lymphocytes in liver tissue of hepatitis B patients.Methods: One hundred cases of hepatitis B patients were divided into mild,moderate and severe hepatitis B groups according to the pathological changes of liver tissues,and normal liver tissue of 15 healthy persons as the control group.CD8+CD28+ and CD8+CD28-T lymphocytes were determined in liver tissue with immunohistochemical double-labeling.Results: The CD8+CD28+ and CD8+CD28-T lymphocytes in hepatitis B patients were significantly higher than those in control group (P<0.05).In hepatitis B patients with mild lesions,CD8+CD28+ T lymphocytes were decreased significantly compared with control group,in hepatitis B patients with moderate and severe lesions,CD8+CD28+ cells and CD8+CD28-T lymphocytes were increased significantly compared with control group (P<0.01).CD8+CD28+ T lymphocytes were raised with the liver inflammation grades,but CD8+CD28-T lymphocytes were reduced gradually with the liver inflammation grades.Conclusions: The expression of CD28 on the surface of CD8+ T lymphocytes in liver tissue of patients with hepatitis B is closely related to liver tissue lesion.The CD8+CD28+ T lymphocytes in liver tissue can be used as immunological markers of liver injury in patients with hepatitis B.CD8+CD28-T lymphocytes in liver tissue may be the main reason for persistent infection of hepatitis B virus.
2010, 35(12): 1296-1298.
Abstract:
Objective: To discuss the cause of false increase in serum MB form creatine kinase (CK-MB) activity in non-cardiogenic patients and its relationship to the disease.Methods: The creatine kinase isoenzyme in the routine specimen of 55 patients whose CK-MB activities were higher than the reference level (25 U/L) and the ratio to creatine kinase (CK) were more than 0.38 were determined using agarose gel electrophoresis.One week later,the CK-MB was determined again and the results were compared with the original values.The analysis was performed combining the clinical diagnosis results.Results: Forty-one of the 55 patients was diagnosed as malignant tumors (16 liver cancer,11 lung carcinoma,7 prostate carcinoma,7 other tumors) and 12 patients as liver cirrhosis;Creatine kinase isoenzyme and the contents of CK-MB of the patients were all less than 5%;Macro-CK2 was detected in 34 cases of malignant tumors,and CK-BB was detected in 23 cases of malignant tumors.Both macro-CK2 and CK-BB were detected in 16 cases of Malignant tumors;Macro-CK2 was detected in 12 cases of liver cirrhosis.Comparison was made between the CK-MB active concentrations detected in the two times.No significant variation was observed (P>0.05).Conclusions: The false increase in serum CK-MB activity is mainly caused by the abnormal increase of CK-BB or macro-CK (macro-CK1,macro-CK2) in blood;their appearance is closely related to liver cirrhosis and certain malignant tumors.
Objective: To discuss the cause of false increase in serum MB form creatine kinase (CK-MB) activity in non-cardiogenic patients and its relationship to the disease.Methods: The creatine kinase isoenzyme in the routine specimen of 55 patients whose CK-MB activities were higher than the reference level (25 U/L) and the ratio to creatine kinase (CK) were more than 0.38 were determined using agarose gel electrophoresis.One week later,the CK-MB was determined again and the results were compared with the original values.The analysis was performed combining the clinical diagnosis results.Results: Forty-one of the 55 patients was diagnosed as malignant tumors (16 liver cancer,11 lung carcinoma,7 prostate carcinoma,7 other tumors) and 12 patients as liver cirrhosis;Creatine kinase isoenzyme and the contents of CK-MB of the patients were all less than 5%;Macro-CK2 was detected in 34 cases of malignant tumors,and CK-BB was detected in 23 cases of malignant tumors.Both macro-CK2 and CK-BB were detected in 16 cases of Malignant tumors;Macro-CK2 was detected in 12 cases of liver cirrhosis.Comparison was made between the CK-MB active concentrations detected in the two times.No significant variation was observed (P>0.05).Conclusions: The false increase in serum CK-MB activity is mainly caused by the abnormal increase of CK-BB or macro-CK (macro-CK1,macro-CK2) in blood;their appearance is closely related to liver cirrhosis and certain malignant tumors.
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