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2010 Vol. 35, No. 3
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2010, 35(3): 217-220.
Abstract:
Objective: To investigate the influence of arsenic trioxide (As2O3) on migration and adhesion capability in melanoma B16 cells,and evaluate the effect of As2O3 on anti-invasion and anti-metastasis ability.Methods: The invasion and migration capacities of melanoma B16 cells were evaluated using Transwell Permeable Supports.The adhesion capacity of melanoma B16 cells was evaluated by MTT assay.The expression of adhesion molecule CD44 was detected by FCM.Results: As2O3 significantly inhibited the adhesion and migration capacities of melanoma B16 cells (P<0.05 to P<0.01) and down-regulated the expression level of CD44 (P<0.01).Conclusions: As2O3 has the effect of antagonizing invasion and metastasis in melanoma B16 cells,the mechanism maybe related to decrease the expression of adhesion molecule CD44.
Objective: To investigate the influence of arsenic trioxide (As2O3) on migration and adhesion capability in melanoma B16 cells,and evaluate the effect of As2O3 on anti-invasion and anti-metastasis ability.Methods: The invasion and migration capacities of melanoma B16 cells were evaluated using Transwell Permeable Supports.The adhesion capacity of melanoma B16 cells was evaluated by MTT assay.The expression of adhesion molecule CD44 was detected by FCM.Results: As2O3 significantly inhibited the adhesion and migration capacities of melanoma B16 cells (P<0.05 to P<0.01) and down-regulated the expression level of CD44 (P<0.01).Conclusions: As2O3 has the effect of antagonizing invasion and metastasis in melanoma B16 cells,the mechanism maybe related to decrease the expression of adhesion molecule CD44.
2010, 35(3): 220-222.
Abstract:
Objective: To study the occurence of apoptosis and the related mechanism of the human umbilical vein endothelial cells (HUVEC) induced by Ureaplasma urealyticum (UU).Methods: The occurence of apoptosis of HUVEC was determined by flow cytometry at different times (2,4,8 and 12 h) after infected by UU.The productions of TNF-α,caspase-3 and caspase-8 in HUVEC after 8-hour infection of UU were detected by colorimetric assay,and the apoptosis rates were determined by flow cytometry when HUVEC was treated with TNF-α antibody,caspase-3 inhibitor or caspase-8 inhibitor prior to UU.Results: UU could induce the apoptosis occurrence of HUVEC with significant difference compared with control group.The productions of TNF-α,caspase-3 and caspase-8 were increased significantly in HUVEC infected by UU in contrast to control group,and the apoptosis occurence was significantly decreased when HUVEC was treated with TNF-α antibody,caspase-3 inhibitor or caspase-8 inhibitor.Conclusions: The results suggested UU could induce the apoptosis occurrence of HUVEC with time-dependence.The mechanism was likely related to extrinsic death pathway dependent on TNF death receptor which induced caspase-8 and caspase-3 activations.
Objective: To study the occurence of apoptosis and the related mechanism of the human umbilical vein endothelial cells (HUVEC) induced by Ureaplasma urealyticum (UU).Methods: The occurence of apoptosis of HUVEC was determined by flow cytometry at different times (2,4,8 and 12 h) after infected by UU.The productions of TNF-α,caspase-3 and caspase-8 in HUVEC after 8-hour infection of UU were detected by colorimetric assay,and the apoptosis rates were determined by flow cytometry when HUVEC was treated with TNF-α antibody,caspase-3 inhibitor or caspase-8 inhibitor prior to UU.Results: UU could induce the apoptosis occurrence of HUVEC with significant difference compared with control group.The productions of TNF-α,caspase-3 and caspase-8 were increased significantly in HUVEC infected by UU in contrast to control group,and the apoptosis occurence was significantly decreased when HUVEC was treated with TNF-α antibody,caspase-3 inhibitor or caspase-8 inhibitor.Conclusions: The results suggested UU could induce the apoptosis occurrence of HUVEC with time-dependence.The mechanism was likely related to extrinsic death pathway dependent on TNF death receptor which induced caspase-8 and caspase-3 activations.
2010, 35(3): 223-225.
Abstract:
Objective: To evaluate the efficiency of a new paraaortic counterpulsation device (PACD) assist therapy in acute heart failure animal modle.Methods: Eight sheep weighing (44.9±2.5)kg were selected.PACD was made by ourself.The blood chambers were connected to descending aorta through a valveless artifical vessel,and air chambers were connected to the intra-aortic ballon pump (IABP) console.The counterpulsation therapy was fulfilled by filling air during heart systole and deflating air during heart diastole.Acute heart failure was induced in all by snaring the left anterior descending coronary artery and (or) branch of left circumflex artery.The hemodynamic effects of PACD were assessed.Hemodynamic parameters during the PACD-assisted beats were compared with those during the unassisted beats to evulate the role of PACD.Results: PACD activation increased cardiac output and mean arterial pressure by 6.29% and 2.04%,respectively,while systole aortic pressure and diastolic aortic pressure were decreased significantly by 3.67% and 8.39%,respectively.The left carotid artery blood flow was increased by 8.12% (P<0.01),and left main coronary artery blood flow was increased by 13.88% (P<0.01).In assistant beats,the peak-augmented diastolic pressure was (97.25±13.67) mmHg,exceeding of unassisted significantly (P<0.01).Conclusions: In acute heart failure model,PACD is an effective counterpulsator which decreases left ventricular afterload and increases hemoperfusion of heart and brain.
Objective: To evaluate the efficiency of a new paraaortic counterpulsation device (PACD) assist therapy in acute heart failure animal modle.Methods: Eight sheep weighing (44.9±2.5)kg were selected.PACD was made by ourself.The blood chambers were connected to descending aorta through a valveless artifical vessel,and air chambers were connected to the intra-aortic ballon pump (IABP) console.The counterpulsation therapy was fulfilled by filling air during heart systole and deflating air during heart diastole.Acute heart failure was induced in all by snaring the left anterior descending coronary artery and (or) branch of left circumflex artery.The hemodynamic effects of PACD were assessed.Hemodynamic parameters during the PACD-assisted beats were compared with those during the unassisted beats to evulate the role of PACD.Results: PACD activation increased cardiac output and mean arterial pressure by 6.29% and 2.04%,respectively,while systole aortic pressure and diastolic aortic pressure were decreased significantly by 3.67% and 8.39%,respectively.The left carotid artery blood flow was increased by 8.12% (P<0.01),and left main coronary artery blood flow was increased by 13.88% (P<0.01).In assistant beats,the peak-augmented diastolic pressure was (97.25±13.67) mmHg,exceeding of unassisted significantly (P<0.01).Conclusions: In acute heart failure model,PACD is an effective counterpulsator which decreases left ventricular afterload and increases hemoperfusion of heart and brain.
2010, 35(3): 226-229.
Abstract:
Objective: To study the relationships between Helicobacter pylori L-form (Hp-L)infection and the invasion and metastasis of gastric carcinoma BGC-823 cells.Further to explore the probable mechanism between Hp-L infection and the progression of gastric carcinoma.Methods: Hp were incubated and induced into Hp-L.Then gastric cancer BGC-823 cells were co-incubated with them for 24 hours in different ratio and a control group was set.The ability of invasion and metastasis of BGC-823 cells were evaluated by using Transwell chambers.The expression of ezrin in BGC-823 cells was assessed by Western-blotting.Results: With the increasing of the concentrations of Hp-L forms,the cells which permeated the membrane were increased (P<0.05 to P<0.01),and the expression of ezrin in gastric carcinoma BGC-823 cells was risen (P<0.01).Conclusions: The infection of Hp-L form can increase the invasion and metastasis ability of gastric carcinoma cells.The mechanism may have relationship with the over-expression of ezrin.
Objective: To study the relationships between Helicobacter pylori L-form (Hp-L)infection and the invasion and metastasis of gastric carcinoma BGC-823 cells.Further to explore the probable mechanism between Hp-L infection and the progression of gastric carcinoma.Methods: Hp were incubated and induced into Hp-L.Then gastric cancer BGC-823 cells were co-incubated with them for 24 hours in different ratio and a control group was set.The ability of invasion and metastasis of BGC-823 cells were evaluated by using Transwell chambers.The expression of ezrin in BGC-823 cells was assessed by Western-blotting.Results: With the increasing of the concentrations of Hp-L forms,the cells which permeated the membrane were increased (P<0.05 to P<0.01),and the expression of ezrin in gastric carcinoma BGC-823 cells was risen (P<0.01).Conclusions: The infection of Hp-L form can increase the invasion and metastasis ability of gastric carcinoma cells.The mechanism may have relationship with the over-expression of ezrin.
2010, 35(3): 229-230.
Abstract:
Objective: To analyze the causes and management of exposed hydroxyapatite (HA) artificial eyes after implantation.Methods: Nine cases of exposed orbital implant were analyzed retrospectively.Different therapies were applied to different cases in accordance to the causes.Amniotic membrane transplantion was performed in 5 cases.Results: All the 9 exposure cases healed well and no recurrence was observed.Conclusions: Several factors may contribute to the implant exposure;proper management and amniotic membrane implantation are effective for the exposure of hydroxyapatite.
Objective: To analyze the causes and management of exposed hydroxyapatite (HA) artificial eyes after implantation.Methods: Nine cases of exposed orbital implant were analyzed retrospectively.Different therapies were applied to different cases in accordance to the causes.Amniotic membrane transplantion was performed in 5 cases.Results: All the 9 exposure cases healed well and no recurrence was observed.Conclusions: Several factors may contribute to the implant exposure;proper management and amniotic membrane implantation are effective for the exposure of hydroxyapatite.
2010, 35(3): 231-234.
Abstract:
Objective: To investigate the expression of caspase-3 protein and Ki-67 in bladder transitional cell carcinomas (BTCC) and its relationship with the grading and staging of the tumor.Methods: The expression of caspase-3 protein and Ki-67 in 55 cases of bladder transitional cell carcinomas and 10 cases of normal bladder mucosas were determined by immuohistochemisitry (SP method).Results: The positive expression of caspase-3 in BTCC (47.3%) was significantly lower than that in normal bladder mucosas (90.0%).The expression level of caspase-3 was positively correlated with the histological grades of BTCC (P<0.05).No positive expression of Ki-67 was observed in normal bladder mucosas,while it was 52.7% in BTCC (P<0.01).The expression of Ki-67 was related positively with the histological grade and negatively with the tumor stage of BTCC (P<0.05).Conclusions: Caspase-3 is closely correlated with the tumorigenesis of BTCC,and may act as a useful marker of invasion of BTCC.Increased expression of Ki-67 maybe related with the genesis and development of BTCC.
Objective: To investigate the expression of caspase-3 protein and Ki-67 in bladder transitional cell carcinomas (BTCC) and its relationship with the grading and staging of the tumor.Methods: The expression of caspase-3 protein and Ki-67 in 55 cases of bladder transitional cell carcinomas and 10 cases of normal bladder mucosas were determined by immuohistochemisitry (SP method).Results: The positive expression of caspase-3 in BTCC (47.3%) was significantly lower than that in normal bladder mucosas (90.0%).The expression level of caspase-3 was positively correlated with the histological grades of BTCC (P<0.05).No positive expression of Ki-67 was observed in normal bladder mucosas,while it was 52.7% in BTCC (P<0.01).The expression of Ki-67 was related positively with the histological grade and negatively with the tumor stage of BTCC (P<0.05).Conclusions: Caspase-3 is closely correlated with the tumorigenesis of BTCC,and may act as a useful marker of invasion of BTCC.Increased expression of Ki-67 maybe related with the genesis and development of BTCC.
2010, 35(3): 234-235.
Abstract:
Objective: To investigate the association of maternal age and environmental factors with the risk of delivering a child with Down's syndrome (DS).Methods: The peripheral blood lymphocytes of the mother of the children with DS were cultured to prepare human G-banding chromosome examples for karyotype analysis,and the association of the maternal age and environmental factors with the risk of delivering a child with DS was analyzed.Results: One hundred and twenty-six of the 145 mothers of children with DS (86.90%) were below the age of 35.All the mothers had contacted with harmful physical or chemical factors to different degree before pregnancy.Conclusions: The age of the mother delivering a child with DS tends to be younger,which is probably due to the harmful environmental factors.Popularizing prepotency knowledge and enlarging the scope of prenatal screening and prenatal diagnosis may decrease the incidence of DS.
Objective: To investigate the association of maternal age and environmental factors with the risk of delivering a child with Down's syndrome (DS).Methods: The peripheral blood lymphocytes of the mother of the children with DS were cultured to prepare human G-banding chromosome examples for karyotype analysis,and the association of the maternal age and environmental factors with the risk of delivering a child with DS was analyzed.Results: One hundred and twenty-six of the 145 mothers of children with DS (86.90%) were below the age of 35.All the mothers had contacted with harmful physical or chemical factors to different degree before pregnancy.Conclusions: The age of the mother delivering a child with DS tends to be younger,which is probably due to the harmful environmental factors.Popularizing prepotency knowledge and enlarging the scope of prenatal screening and prenatal diagnosis may decrease the incidence of DS.
2010, 35(3): 236-238.
Abstract:
Objective: To investigate the method of modified pudendal thigh flaps transfer for vaginoplasty.Methods: The modified pudendal thigh flaps were formed which the subcutaneous fat of half distal end was clipped and subdermal vascular network was conserved.Results: All five cases of flaps were survived with primary healing.The flaps had no overcrowding and scar.The reconstruction vaginoplasty was spacious which the width was two fingers and the depth was about 10-12 cm after 6 months to 1 year.Conclusions: The modified pudendal thigh flaps used in vaginal reconstruction are thin and have rich blood supply,the contour of the vulva is satisfied.The lacuna is enlarged and not shift outward easily.
Objective: To investigate the method of modified pudendal thigh flaps transfer for vaginoplasty.Methods: The modified pudendal thigh flaps were formed which the subcutaneous fat of half distal end was clipped and subdermal vascular network was conserved.Results: All five cases of flaps were survived with primary healing.The flaps had no overcrowding and scar.The reconstruction vaginoplasty was spacious which the width was two fingers and the depth was about 10-12 cm after 6 months to 1 year.Conclusions: The modified pudendal thigh flaps used in vaginal reconstruction are thin and have rich blood supply,the contour of the vulva is satisfied.The lacuna is enlarged and not shift outward easily.
2010, 35(3): 238-239.
Abstract:
Objective: To evaluate the surgical effect of dismembered pyeloplasty on ureteropelvic junction (UPJ) stricture.Methods: A total of 32 cases of UPJ stricture were performed dismembered pyeloplasty with double pig-tail catheters.The double pig-tail catheter was removed 6-8 weeks after the operation.The outcome was evaluated 3-6 months later.Results: The operation was successful in all the 32 cases.Intravenous pelviureterograghy showed that the hydronephrosis was improved,the stricture relieved and the anastomotic stoma not obstructed.All the patients were followed up for six months.No complication occurred.Conclusions: Dismembered pyeloplasty is an effective method for treatment of UPJ stricture.Double pig-tail placement during operation can reduce the chance of UPJ restricture.
Objective: To evaluate the surgical effect of dismembered pyeloplasty on ureteropelvic junction (UPJ) stricture.Methods: A total of 32 cases of UPJ stricture were performed dismembered pyeloplasty with double pig-tail catheters.The double pig-tail catheter was removed 6-8 weeks after the operation.The outcome was evaluated 3-6 months later.Results: The operation was successful in all the 32 cases.Intravenous pelviureterograghy showed that the hydronephrosis was improved,the stricture relieved and the anastomotic stoma not obstructed.All the patients were followed up for six months.No complication occurred.Conclusions: Dismembered pyeloplasty is an effective method for treatment of UPJ stricture.Double pig-tail placement during operation can reduce the chance of UPJ restricture.
2010, 35(3): 240-242.
Abstract:
Objective: To investigate the efficacy of mitral valve replacement (MVR) by continuous suture with the heart beating under cardiopulmonary bypass (CPB) with mild hypothermia by continuous suture.Methods: Ninety-seven cases of rheumatic heart disease were performed MVR by continuous suture with the heart beating under CPB with mild hypothermia.The superior vena cava and inferior vena cava were blocked with the heart beating through the right atrium-septum in operation.The aspirater was augmentated to keep the view clear in operation.two-0 Prolene suture line was designed for continuous suture in MVR.Affiliate operation included 29 cases of De Vega's tricuspid annuloplast and 5 cases of removing atria sinistrum thrombus.Results: After operation,one patient developed perivalvular leakage,which was repaired 28 h after the first operation;And the other patients were discharged on due time.No low cardiac output syndrome,serious cardiac arrhythmias,air embolism or thrombus occurred.The efficacy was satisfactory during the 2-24 month follow-up.Conclusions: MVR by continuous suture with the heart beating under CPB with mild hypothermia may shorten the operation time,and reduce the complications by adopting the myocardial protection method,which approaches the physiology states and proves effective.
Objective: To investigate the efficacy of mitral valve replacement (MVR) by continuous suture with the heart beating under cardiopulmonary bypass (CPB) with mild hypothermia by continuous suture.Methods: Ninety-seven cases of rheumatic heart disease were performed MVR by continuous suture with the heart beating under CPB with mild hypothermia.The superior vena cava and inferior vena cava were blocked with the heart beating through the right atrium-septum in operation.The aspirater was augmentated to keep the view clear in operation.two-0 Prolene suture line was designed for continuous suture in MVR.Affiliate operation included 29 cases of De Vega's tricuspid annuloplast and 5 cases of removing atria sinistrum thrombus.Results: After operation,one patient developed perivalvular leakage,which was repaired 28 h after the first operation;And the other patients were discharged on due time.No low cardiac output syndrome,serious cardiac arrhythmias,air embolism or thrombus occurred.The efficacy was satisfactory during the 2-24 month follow-up.Conclusions: MVR by continuous suture with the heart beating under CPB with mild hypothermia may shorten the operation time,and reduce the complications by adopting the myocardial protection method,which approaches the physiology states and proves effective.
2010, 35(3): 242-243.
Abstract:
Objective: To investigate the efficiency of comprehensive therapy on cracked teeth.Methods: Ninety-two cracked teeth were treated by comprehensive therapy,including occlusal adjustment,band stabilization,root canal treatment,filling,and all-crown restoration.All the patients were followed up for 1-3 years observed the symptoms and sign of the patients.Results: Eighty-four cases were cured and the success rate was 91.30%.The reason for most failure cases was improper time for treatment.Conclusions: Comprehensive therapy is an ideal method of treating cracked teeth for keeping tooth tissues as for as possible.
Objective: To investigate the efficiency of comprehensive therapy on cracked teeth.Methods: Ninety-two cracked teeth were treated by comprehensive therapy,including occlusal adjustment,band stabilization,root canal treatment,filling,and all-crown restoration.All the patients were followed up for 1-3 years observed the symptoms and sign of the patients.Results: Eighty-four cases were cured and the success rate was 91.30%.The reason for most failure cases was improper time for treatment.Conclusions: Comprehensive therapy is an ideal method of treating cracked teeth for keeping tooth tissues as for as possible.
2010, 35(3): 244-245.
Abstract:
Objective: To investigate and analyze the clinical factors related to hematoma enlargement after acute stage of hypertensive cerebral hemorrhage.Methods: One hundred and seventy-eight patients at the acute stage of hypertensive cerebral hemorrhage in our department were divided into two groups.Forty-one patients with hematoma enlargement were included in the observation group,the others acted as control.The clinical data related to hematoma enlargement of the two groups were reviewed retrospectively.Results: There was significant difference between the two groups in symptoms such as vomiting,uneven CT density and the ratio of insobriety (P<0.01),while no difference was observed in the number of patients with or without diabetes between the two groups (P>0.05).Conclusions: Vomiting,uneven density,hematoma and chronic alcohol consumption may be related to the hematoma enlargement after acute stage of hypertensive cerebral hemorrhage.
Objective: To investigate and analyze the clinical factors related to hematoma enlargement after acute stage of hypertensive cerebral hemorrhage.Methods: One hundred and seventy-eight patients at the acute stage of hypertensive cerebral hemorrhage in our department were divided into two groups.Forty-one patients with hematoma enlargement were included in the observation group,the others acted as control.The clinical data related to hematoma enlargement of the two groups were reviewed retrospectively.Results: There was significant difference between the two groups in symptoms such as vomiting,uneven CT density and the ratio of insobriety (P<0.01),while no difference was observed in the number of patients with or without diabetes between the two groups (P>0.05).Conclusions: Vomiting,uneven density,hematoma and chronic alcohol consumption may be related to the hematoma enlargement after acute stage of hypertensive cerebral hemorrhage.
2010, 35(3): 246-248.
Abstract:
Objective: To investigate the effect of small incision non-phacoemulcification cataract extraction with intraocular lens (IOL) implantation in large scale vision recovery action.Methods: Four hundred and ninety-six cases (496 eyes) of cataract were treated by small incision non-phacoemulcification cataract extraction with IOL implantation.Results: The visual acuity reached 0.3 or more in 440 eyes one day after the operation,and 464 eyes had a visual acuity better than 0.3 seven days after the operation.Conclusions: Small incision non-phacoemulcification cataract surgery and IOL implantation is simple,effective,economical and safe,adapting for large scale vision recovery action.
Objective: To investigate the effect of small incision non-phacoemulcification cataract extraction with intraocular lens (IOL) implantation in large scale vision recovery action.Methods: Four hundred and ninety-six cases (496 eyes) of cataract were treated by small incision non-phacoemulcification cataract extraction with IOL implantation.Results: The visual acuity reached 0.3 or more in 440 eyes one day after the operation,and 464 eyes had a visual acuity better than 0.3 seven days after the operation.Conclusions: Small incision non-phacoemulcification cataract surgery and IOL implantation is simple,effective,economical and safe,adapting for large scale vision recovery action.
2010, 35(3): 248-249.
Abstract:
Objective: To observe the effect of septoplasty and partial turbinectomy on the peak flow of nose,and to evaluate the value of the peak flow of nose in the diagnosis of the nasal chronic diseases.Methods: The peak flow of septoplasty and partial turbinectomy pre-and post-operation was measured using Mini Wright peak flow meter.Results: The peak flow of nose in normal adults was stable before and after 4 weeks.The peak flow of nose in the patients who were performed with septoplasty was increased to the normal level after operation.There are significant differences between pre-and post-operation (P<0.01).The peak flow of nose in the patients who were performed with partial turbinectomy was increased to the normal level as well after operation.There was significant difference between pre-and post-operation (P<0.01).Conclusions: Septoplasty and partial turbinectomy can increase the peak flow of nose significantly and improve the nasal airway function.
Objective: To observe the effect of septoplasty and partial turbinectomy on the peak flow of nose,and to evaluate the value of the peak flow of nose in the diagnosis of the nasal chronic diseases.Methods: The peak flow of septoplasty and partial turbinectomy pre-and post-operation was measured using Mini Wright peak flow meter.Results: The peak flow of nose in normal adults was stable before and after 4 weeks.The peak flow of nose in the patients who were performed with septoplasty was increased to the normal level after operation.There are significant differences between pre-and post-operation (P<0.01).The peak flow of nose in the patients who were performed with partial turbinectomy was increased to the normal level as well after operation.There was significant difference between pre-and post-operation (P<0.01).Conclusions: Septoplasty and partial turbinectomy can increase the peak flow of nose significantly and improve the nasal airway function.
2010, 35(3): 250-251.
Abstract:
Objective: To observe the therapeutic and toxic effect of cisplatin combined with lentinan in treatment of malignant pleural effusion.Methods: A drainage tube was placed into the chest to release the pleural effusion.The observation group were administered intrapleural injection of cisplatin 40 mg and lentinan 4 mg alternately,and the control group intrapleural cisplatin 60 mg only.The therapy was repeated 3-7 day;one course consisted of 4 times.The efficacy and toxicity were observed 1 month after the regimen.Results: The observation group had a better short-term effect and life quality than the control group (P<0.05 and P<0.01);the observation group demonstrated lower toxicity reaction than the control group (P<0.05).Conclusions: Lentinan combined with cisplatin therapy can enhance the efficacy,reduce the side effects and improve the quality of life of the patients with malignant pleural effusion.
Objective: To observe the therapeutic and toxic effect of cisplatin combined with lentinan in treatment of malignant pleural effusion.Methods: A drainage tube was placed into the chest to release the pleural effusion.The observation group were administered intrapleural injection of cisplatin 40 mg and lentinan 4 mg alternately,and the control group intrapleural cisplatin 60 mg only.The therapy was repeated 3-7 day;one course consisted of 4 times.The efficacy and toxicity were observed 1 month after the regimen.Results: The observation group had a better short-term effect and life quality than the control group (P<0.05 and P<0.01);the observation group demonstrated lower toxicity reaction than the control group (P<0.05).Conclusions: Lentinan combined with cisplatin therapy can enhance the efficacy,reduce the side effects and improve the quality of life of the patients with malignant pleural effusion.
2010, 35(3): 252-254.
Abstract:
Objective: To investigate the significance of HLA-B27 in the progression and prognosis of patients with undifferentiated spondyloarthropathy (uSpA).Methods: The clinical and laboratory manifestations of 78 uSpA patients with positive HLA-B27 were analyzed and compared with those of 42 patients wit negative HLA-B27.Some of the patients were followed up for 3 years to observe the changes of pathogenetic condition.Results: The HLA-B27 (+) patients had an earlier onset of disease (P<0.05),and more HLA-B27 (+) patients developed general symptoms including fever,weight loss and languor (P<0.05).In the laboratory examination,the erythrocyte sedimentation rates of HLA-B27 (+)group were also increased (P<0.05).There was significant difference in X-ray manifestations between the two groups (P<0.05).One year later,more patients in HLA-B27 (+)group developed ankylosing spondylitis (AS) (P<0.05).Conclusions: HLA-B27 (+) uSpA patients have severer symptoms and a higher rate of developing AS than HLA-B27 (-) patients.HLA-B27 plays an important role in the pathogenesis of uSpA.
Objective: To investigate the significance of HLA-B27 in the progression and prognosis of patients with undifferentiated spondyloarthropathy (uSpA).Methods: The clinical and laboratory manifestations of 78 uSpA patients with positive HLA-B27 were analyzed and compared with those of 42 patients wit negative HLA-B27.Some of the patients were followed up for 3 years to observe the changes of pathogenetic condition.Results: The HLA-B27 (+) patients had an earlier onset of disease (P<0.05),and more HLA-B27 (+) patients developed general symptoms including fever,weight loss and languor (P<0.05).In the laboratory examination,the erythrocyte sedimentation rates of HLA-B27 (+)group were also increased (P<0.05).There was significant difference in X-ray manifestations between the two groups (P<0.05).One year later,more patients in HLA-B27 (+)group developed ankylosing spondylitis (AS) (P<0.05).Conclusions: HLA-B27 (+) uSpA patients have severer symptoms and a higher rate of developing AS than HLA-B27 (-) patients.HLA-B27 plays an important role in the pathogenesis of uSpA.
2010, 35(3): 254-256.
Abstract:
Objective: To analyze the clinic features of viral encephalitis caused by hand-foot-mouth disease (HFMD),and to explore its diagnosis,treatment and prognosis.Methods: Sixty-five cases of HFMD were diagnosed and methyllprednisolone,gamma globulin assaulting,dewatering and resisting virus therapies were applied.Results: The major clinical manifestations of the 65 cases were skin rash,fever,drowsiness,limbs shaking and emesia.The symptoms of viral encephalitis appeared on the second to the fourth day following the onset of the disease.Sixty-three cases were cured and 2 cases had nervous system residual when discharged.Conclusions: The viral encephalitis caused by HFMD is most frequently observed in children below the age of three.The symptoms of nerve system are not typital.Early diagnosis and treatment are essential to successful remedy.Delayed treatment may lead to never system residual and death.
Objective: To analyze the clinic features of viral encephalitis caused by hand-foot-mouth disease (HFMD),and to explore its diagnosis,treatment and prognosis.Methods: Sixty-five cases of HFMD were diagnosed and methyllprednisolone,gamma globulin assaulting,dewatering and resisting virus therapies were applied.Results: The major clinical manifestations of the 65 cases were skin rash,fever,drowsiness,limbs shaking and emesia.The symptoms of viral encephalitis appeared on the second to the fourth day following the onset of the disease.Sixty-three cases were cured and 2 cases had nervous system residual when discharged.Conclusions: The viral encephalitis caused by HFMD is most frequently observed in children below the age of three.The symptoms of nerve system are not typital.Early diagnosis and treatment are essential to successful remedy.Delayed treatment may lead to never system residual and death.
2010, 35(3): 257-259.
Abstract:
Objective: To explore the clinical application of sural neurovasculr pedicle faseiocutaneous reversed island flap in repairing soft-tissue defection in the inferior segment of the leg,ankle and foot.Methods: Sixteen cases of soft-tissue defects in the ankle and foot were repaired using sural neurovaseular faseiocutaneous reversed island flap.The flap area ranged from 5 cm×7 cm to 18 cm×14 cm and the length of the pedicle 5 cm to 16 cm.Results: All the 14 flaps survived.The distal part necrosed partially in 2 cases and healed after secondary free-skin grafting.All the ceases were followed up for 3 to 6 months.The contour appearance,color and tenacity of the flaps were excellent;the function of the ankle joints was good.Conclusions: The sural neurovasculr pedicle faseiocutaneous reversed island flap has a reliable blood supp1y.It is easy to operate and has a high survival rate,with no harm to the major arteries.It can be used to repair the soft tissue defects in the inferior part of the leg,ankle and foot.
Objective: To explore the clinical application of sural neurovasculr pedicle faseiocutaneous reversed island flap in repairing soft-tissue defection in the inferior segment of the leg,ankle and foot.Methods: Sixteen cases of soft-tissue defects in the ankle and foot were repaired using sural neurovaseular faseiocutaneous reversed island flap.The flap area ranged from 5 cm×7 cm to 18 cm×14 cm and the length of the pedicle 5 cm to 16 cm.Results: All the 14 flaps survived.The distal part necrosed partially in 2 cases and healed after secondary free-skin grafting.All the ceases were followed up for 3 to 6 months.The contour appearance,color and tenacity of the flaps were excellent;the function of the ankle joints was good.Conclusions: The sural neurovasculr pedicle faseiocutaneous reversed island flap has a reliable blood supp1y.It is easy to operate and has a high survival rate,with no harm to the major arteries.It can be used to repair the soft tissue defects in the inferior part of the leg,ankle and foot.
2010, 35(3): 260-262.
Abstract:
Objective: To investigate the relationship between cervical erosion and infection caused by Ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) in female infertility.Methods: The UU and CT in 169 infertile women were determined by PCR.Sixty-one cases with cervical erosion were enrolled in the observation group and the other 108 cases acted as normal control.Results: The infection rates were 65.57% and 85.19% in the observation group and the control,respectively.The difference was significant (P<0.05).The overall infection rates of UU and CT were 69.82% and 30.18% (P<0.01),respectively.Conclusions: The infection of UU is the main factor for infertility.
Objective: To investigate the relationship between cervical erosion and infection caused by Ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) in female infertility.Methods: The UU and CT in 169 infertile women were determined by PCR.Sixty-one cases with cervical erosion were enrolled in the observation group and the other 108 cases acted as normal control.Results: The infection rates were 65.57% and 85.19% in the observation group and the control,respectively.The difference was significant (P<0.05).The overall infection rates of UU and CT were 69.82% and 30.18% (P<0.01),respectively.Conclusions: The infection of UU is the main factor for infertility.
2010, 35(3): 262-263.
Abstract:
Objective: To assess the value of laparoscopy in diagnosis and treatment of chronic pelvic pain (CPP).Methods: One hundred and twenty six patients with CPP were examined and treated by laproscopy.Results: Of the 126 cases,58 had endometriosis,36 chronic pelvic inflammatory disease,13 pelvic adhesions,9 pelvic venous congestion syndrome and 7 other diseases;3 cases had no found abnormal under laparoscopy.Conclusions: As the causes of CPP are complicated,it is difficult to make diagnosis.Laparoscopy is capable of defining the causes of most CPP and providing treatment at the same time,and the first choice for diagnosis and treatment of CPP.
Objective: To assess the value of laparoscopy in diagnosis and treatment of chronic pelvic pain (CPP).Methods: One hundred and twenty six patients with CPP were examined and treated by laproscopy.Results: Of the 126 cases,58 had endometriosis,36 chronic pelvic inflammatory disease,13 pelvic adhesions,9 pelvic venous congestion syndrome and 7 other diseases;3 cases had no found abnormal under laparoscopy.Conclusions: As the causes of CPP are complicated,it is difficult to make diagnosis.Laparoscopy is capable of defining the causes of most CPP and providing treatment at the same time,and the first choice for diagnosis and treatment of CPP.
2010, 35(3): 264-266.
Abstract:
Objective: To analyze the related factors of nosocomial infection,the pathogen distribution and drug resistance in patients with lung cancer,so as to take effective measures and provide basis for rational use of drugs.Methods: The nosocomial infection in 328 cases of lung cancer patients were analyzed retrospectively.Results: Among the 328 cases of lung cancer,nosocomial infection occurred in 63 cases,and 96 times,with a rate of 19.21% (in cases) and 29.27% (in times),respectively;while it was 4.26% in other patients in the corresponding period of time.The infection mostly occurred in patients with lung cancer at the intermediate stage and the terminal stage.Severe malnutrition,complications and invasive operation were the predominant susceptibility factors.A total of 142 samples were submitted to detection,and pathogens were detected in 96 of them including 63 strains of G- bacillus,24 strains of G+ cocci and 9 strains of fungi.The infection occurred mainly in the lower respiratory tract,and secondly in the digestive tract.Conclusions: There is a high incidence of nosocomial infection in patients with lung cancer.Susceptibility factors are varied.The main site of infection is the lower respiratory tract;the dominating pathogenic bacteria are G- bacillus;bacterial resistance increases significantly.Rational use of antimicrobial agents may reduce the incidence of nosocomial infections and drug resistance.
Objective: To analyze the related factors of nosocomial infection,the pathogen distribution and drug resistance in patients with lung cancer,so as to take effective measures and provide basis for rational use of drugs.Methods: The nosocomial infection in 328 cases of lung cancer patients were analyzed retrospectively.Results: Among the 328 cases of lung cancer,nosocomial infection occurred in 63 cases,and 96 times,with a rate of 19.21% (in cases) and 29.27% (in times),respectively;while it was 4.26% in other patients in the corresponding period of time.The infection mostly occurred in patients with lung cancer at the intermediate stage and the terminal stage.Severe malnutrition,complications and invasive operation were the predominant susceptibility factors.A total of 142 samples were submitted to detection,and pathogens were detected in 96 of them including 63 strains of G- bacillus,24 strains of G+ cocci and 9 strains of fungi.The infection occurred mainly in the lower respiratory tract,and secondly in the digestive tract.Conclusions: There is a high incidence of nosocomial infection in patients with lung cancer.Susceptibility factors are varied.The main site of infection is the lower respiratory tract;the dominating pathogenic bacteria are G- bacillus;bacterial resistance increases significantly.Rational use of antimicrobial agents may reduce the incidence of nosocomial infections and drug resistance.
2010, 35(3): 266-268.
Abstract:
Objective: To investigate the expressions of CD44v6 and PTEN in renal cell carcinomal (RCC) and its clinical significance.Methods: The expressions of CD44v6 and PTEN were examined by immunohistochemistry S-P method in 20 cases of Para carcinoma,10 cases of normal renal tissues and 60 cases of RCC;And the pathobiologic behavior was analyzed.Results: The positive rates of CD44v6 and PTEN in RCC were 51.7% and 66.7%,respectively.The histological grading,clinical staging and metastases potential of RCC were correlated with high expression of CD44v6 and low expression of PTEN (P<0.01).The expression of CD44v6 was negatively correlated with the expression of PTEN (P<0.05-P<0.01).Conclusions: The result suggests that high expression of CD44v6 and low expression of PTEN might be a useful indicator for evaluation of the differentiation of tumors and prediction of lymph nodes metastasis.
Objective: To investigate the expressions of CD44v6 and PTEN in renal cell carcinomal (RCC) and its clinical significance.Methods: The expressions of CD44v6 and PTEN were examined by immunohistochemistry S-P method in 20 cases of Para carcinoma,10 cases of normal renal tissues and 60 cases of RCC;And the pathobiologic behavior was analyzed.Results: The positive rates of CD44v6 and PTEN in RCC were 51.7% and 66.7%,respectively.The histological grading,clinical staging and metastases potential of RCC were correlated with high expression of CD44v6 and low expression of PTEN (P<0.01).The expression of CD44v6 was negatively correlated with the expression of PTEN (P<0.05-P<0.01).Conclusions: The result suggests that high expression of CD44v6 and low expression of PTEN might be a useful indicator for evaluation of the differentiation of tumors and prediction of lymph nodes metastasis.
2010, 35(3): 269-270.
Abstract:
Objective: To observe the advantages and disadvantages of continuous negative pressure drainage and compacted rubber cross drainage in parotid gland surgery.Methods: Fifty-one patients with parotid gland were randomly divided into two groups:continuous negative pressure drainage group (group A,26 cases) and compacted rubber cross drainage group (group B,25 cases).The subjective comments of the patients and the clinic effects were taken into comparative analyses.Results: Group A had the advantages of slighter pain,less interference with food intake,less complication and shorter hospital stay as compared with group B (P<0.01).Conclusions: Negative pressure drainage is superior to traditional rubber cross drainage in parotid gland surgery.
Objective: To observe the advantages and disadvantages of continuous negative pressure drainage and compacted rubber cross drainage in parotid gland surgery.Methods: Fifty-one patients with parotid gland were randomly divided into two groups:continuous negative pressure drainage group (group A,26 cases) and compacted rubber cross drainage group (group B,25 cases).The subjective comments of the patients and the clinic effects were taken into comparative analyses.Results: Group A had the advantages of slighter pain,less interference with food intake,less complication and shorter hospital stay as compared with group B (P<0.01).Conclusions: Negative pressure drainage is superior to traditional rubber cross drainage in parotid gland surgery.
2010, 35(3): 271-272.
Abstract:
Objective: To investigate the efficacy of surgery on delayed traumatic rupture of spleen,and to summarize the experience in the diagnosis and treatment.Methods: Seventeen cases of delayed traumatic rupture of spleen were treated by surgical therapy.Ten cases were performed splenectomy,5 cases spleen repair and 2 cases splenic artery ligation plus partial spleen resection.Results: Sixteen of the patients were cure and 1 died.Conclusions: Patients with delayed traumatic rupture of spleen should be closely observed and monitored.Timely diagnosis is essential.Surgery remains the choice therapy.Spleen-preserving should be considered in the treatment.
Objective: To investigate the efficacy of surgery on delayed traumatic rupture of spleen,and to summarize the experience in the diagnosis and treatment.Methods: Seventeen cases of delayed traumatic rupture of spleen were treated by surgical therapy.Ten cases were performed splenectomy,5 cases spleen repair and 2 cases splenic artery ligation plus partial spleen resection.Results: Sixteen of the patients were cure and 1 died.Conclusions: Patients with delayed traumatic rupture of spleen should be closely observed and monitored.Timely diagnosis is essential.Surgery remains the choice therapy.Spleen-preserving should be considered in the treatment.
2010, 35(3): 273-275.
Abstract:
Objective: To explore the procedures for treatment of complications of arteriovenous fistula in patients receiving maintenance hemodialysis.Methods: Three hundred and nineteen patients receiving maintenance hemodialysis were included in the study.The fistula surgery,puncture process,main complications and treatment procedures were analyzed.Results: The arterioveneous fistula in all the 319 case was built mainly by terminal-terminal anastomosis in the unaccustomedly-used forearm with "button-like and ladder-type puncture".The most common complications were thrombus (26%) including anastomotic thrombosis (19.7%) and puncture site mural thrombus (6.3%),vascular access stenosis (10.3%) including anastomotic stenosis,vein anatomy inherent stenosis and narrow puncture site,and hemangioma which mainly occurred in the stoma and the puncture site (3.8%).Of the 319 fistula,234 were used for more than 3 years.Conclusions: The reasons for fistula vascular access dysfunction in maintenance hemodialysis patients are thrombus,stenosis and hemangioma in sequence,and surgery is the most effective therapy for complications of vascular access.
Objective: To explore the procedures for treatment of complications of arteriovenous fistula in patients receiving maintenance hemodialysis.Methods: Three hundred and nineteen patients receiving maintenance hemodialysis were included in the study.The fistula surgery,puncture process,main complications and treatment procedures were analyzed.Results: The arterioveneous fistula in all the 319 case was built mainly by terminal-terminal anastomosis in the unaccustomedly-used forearm with "button-like and ladder-type puncture".The most common complications were thrombus (26%) including anastomotic thrombosis (19.7%) and puncture site mural thrombus (6.3%),vascular access stenosis (10.3%) including anastomotic stenosis,vein anatomy inherent stenosis and narrow puncture site,and hemangioma which mainly occurred in the stoma and the puncture site (3.8%).Of the 319 fistula,234 were used for more than 3 years.Conclusions: The reasons for fistula vascular access dysfunction in maintenance hemodialysis patients are thrombus,stenosis and hemangioma in sequence,and surgery is the most effective therapy for complications of vascular access.
2010, 35(3): 286-288.
Abstract:
Objective: To explore the methodology for epidemiology teaching in meeting the social needs for talents in epidemiology and cultivate more qualified talents in public health.Methods: A serious of measures were implemented in teaching reforms including increasing students' interest in epidemiology,enhancing faculty construction and teaching management,improving the epidemiological activities and teaching methods.Results: The quality of epidemiology teaching was improved through adjustment and completion of epidemiology teaching reforms.Conclusions: Through a series of reform measures for epidemiology teaching,students had strengthened their mastery of theoretical knowledge and application of knowledge in practice;meanwhile,students' ability in scientific research has been improved as well.
Objective: To explore the methodology for epidemiology teaching in meeting the social needs for talents in epidemiology and cultivate more qualified talents in public health.Methods: A serious of measures were implemented in teaching reforms including increasing students' interest in epidemiology,enhancing faculty construction and teaching management,improving the epidemiological activities and teaching methods.Results: The quality of epidemiology teaching was improved through adjustment and completion of epidemiology teaching reforms.Conclusions: Through a series of reform measures for epidemiology teaching,students had strengthened their mastery of theoretical knowledge and application of knowledge in practice;meanwhile,students' ability in scientific research has been improved as well.
2010, 35(3): 290-292.
Abstract:
Objective: To provide a simple and useful method of verifying the accuracy position of the radiation fields for three dimensional conformal radiotherapy plan.Methods: A simulator was used to take the radiography of 204 cases of conformal fields planned by 3DCRT;the conformal field radiography was transported to LANTIS system and compared with the planning-built fields;their coincidence and causes of error were observed and analyzed.Results: For the tumors on head and neck,the conformal field coincided well with the planning-built fields,and the errors in XY position was within 5 mm,but for tumors in chest and abdomen,the biggest error of field position was 12 mm.Conclusions: The method has the advantages of easy operation,low cost and practicability,adapting to the sections in middle-class hospitals using 3DCRT plan.The simulator verification can observe the tumor target and its nearby organs in risk at the same time.
Objective: To provide a simple and useful method of verifying the accuracy position of the radiation fields for three dimensional conformal radiotherapy plan.Methods: A simulator was used to take the radiography of 204 cases of conformal fields planned by 3DCRT;the conformal field radiography was transported to LANTIS system and compared with the planning-built fields;their coincidence and causes of error were observed and analyzed.Results: For the tumors on head and neck,the conformal field coincided well with the planning-built fields,and the errors in XY position was within 5 mm,but for tumors in chest and abdomen,the biggest error of field position was 12 mm.Conclusions: The method has the advantages of easy operation,low cost and practicability,adapting to the sections in middle-class hospitals using 3DCRT plan.The simulator verification can observe the tumor target and its nearby organs in risk at the same time.
2010, 35(3): 292-294.
Abstract:
Objective: To explore the clinical effect of selective arteria uterine cannula chemotherapy embolism on tubal pregnancy.Methods: Twenty-seven cases of tubal pregnancy were performed arteria uterine visualization with advanced Seldinger technique.The affected arteria uterine was injected methotrexate (one case was injected Fluorouracil) through a catheter and filled with gel foam embolism.The clinical symptoms and signs,serum human chorionic gonadotropin,pelvic hemataocele changes and absorption of pelvic hydrops were observed after the surgery.Results: The arteria uterine cannula was successful in all the 27 cases.Twenty-six of them were cured and 1 failed.The cure rate was 96.3%.No complication occurred.Four patients had healthy babies 3-10 months after the surgery.Conclusions: The new method has the advantages of small wounds and a higher cure rate,which may effectively prevent intraperitonea bleeding,keep the integrity of the arteria uterine and thus retains the fertility.
Objective: To explore the clinical effect of selective arteria uterine cannula chemotherapy embolism on tubal pregnancy.Methods: Twenty-seven cases of tubal pregnancy were performed arteria uterine visualization with advanced Seldinger technique.The affected arteria uterine was injected methotrexate (one case was injected Fluorouracil) through a catheter and filled with gel foam embolism.The clinical symptoms and signs,serum human chorionic gonadotropin,pelvic hemataocele changes and absorption of pelvic hydrops were observed after the surgery.Results: The arteria uterine cannula was successful in all the 27 cases.Twenty-six of them were cured and 1 failed.The cure rate was 96.3%.No complication occurred.Four patients had healthy babies 3-10 months after the surgery.Conclusions: The new method has the advantages of small wounds and a higher cure rate,which may effectively prevent intraperitonea bleeding,keep the integrity of the arteria uterine and thus retains the fertility.
2010, 35(3): 295-297.
Abstract:
Objective: To study the speed model of the veins of lower extremities in the magnetic resonance angiography (MRA),and to provide basic reference for clinical practice.Methods: The relationship between the flow speeds and the signal changes in MRA was analyzed.Results: The signal was different between static and flowing fluids on magnetic resonance machine when the speed was 0.4 mm/s (P<0.05),and the changes could not be differentiated by the naked eye.When the flowing speed was 1.2 mm/s,the signal changes could be clearly differentiated by the naked eye.Conclusions: Different flowing speed has different influence on MRA imaging.MRA is convenient and feasible in diagnosis of diseases in the lower extremities.
Objective: To study the speed model of the veins of lower extremities in the magnetic resonance angiography (MRA),and to provide basic reference for clinical practice.Methods: The relationship between the flow speeds and the signal changes in MRA was analyzed.Results: The signal was different between static and flowing fluids on magnetic resonance machine when the speed was 0.4 mm/s (P<0.05),and the changes could not be differentiated by the naked eye.When the flowing speed was 1.2 mm/s,the signal changes could be clearly differentiated by the naked eye.Conclusions: Different flowing speed has different influence on MRA imaging.MRA is convenient and feasible in diagnosis of diseases in the lower extremities.
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