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2010 Vol. 35, No. 6
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2010, 35(6): 541-544,547.
Abstract:
Objective:To establish a method for detection of reactive oxygen species (ROS) of human polymorphonuclear cells (PMN) by flow cytometry.Methods:The whole fresh peripheral blood or Percoll separated PMN were incubated with dihydrogenrhodamine 123 (DHR123) or 2',7'-Dichlorodihydrofluorescin diacetate (DCFH-DA) as fluorescence probes for 15 min,followed adding PMA for 5-90 minutes at 37℃.The fluorescence intensities of rhodamine 123 and dichlorofluorecin were detected by flow cytometry,which reflecting the amount of reactive oxygen species within PMN.Results:By using DHR123 as a fluorescence probe,the tendency of the production of ROS generated in whole blood or separated PMN was similar when PMA incubated with them at 37℃,the amount of ROS were increased from 5 min to 60 min,reached a peak at 60 min,and decreased between 60 min to 90 min.By using DCFH-DA as fluorescent probe,the amount of ROS generated in whole blood stimulated by PMA reached the maximum in 5 min and thereafter decreased rapidly,whereas the production of ROS generated by isolated PMN increased depended on the time from 5 min to 30 min and reached platform between 60 min to 90 min;the RHO fluorescence intensity was apparently inhibited by formaldehyde-containing reagents.Conclusions:The application of flow cytometry to measure the reactive oxygen species within the neutrophils is simple,reliable and stable method.
Objective:To establish a method for detection of reactive oxygen species (ROS) of human polymorphonuclear cells (PMN) by flow cytometry.Methods:The whole fresh peripheral blood or Percoll separated PMN were incubated with dihydrogenrhodamine 123 (DHR123) or 2',7'-Dichlorodihydrofluorescin diacetate (DCFH-DA) as fluorescence probes for 15 min,followed adding PMA for 5-90 minutes at 37℃.The fluorescence intensities of rhodamine 123 and dichlorofluorecin were detected by flow cytometry,which reflecting the amount of reactive oxygen species within PMN.Results:By using DHR123 as a fluorescence probe,the tendency of the production of ROS generated in whole blood or separated PMN was similar when PMA incubated with them at 37℃,the amount of ROS were increased from 5 min to 60 min,reached a peak at 60 min,and decreased between 60 min to 90 min.By using DCFH-DA as fluorescent probe,the amount of ROS generated in whole blood stimulated by PMA reached the maximum in 5 min and thereafter decreased rapidly,whereas the production of ROS generated by isolated PMN increased depended on the time from 5 min to 30 min and reached platform between 60 min to 90 min;the RHO fluorescence intensity was apparently inhibited by formaldehyde-containing reagents.Conclusions:The application of flow cytometry to measure the reactive oxygen species within the neutrophils is simple,reliable and stable method.
2010, 35(6): 545-547.
Abstract:
Objective:To clone genes for encoding human interleukin-23 (IL-23) p19 subunit.Methods:Peripheral blood mononuclear cells (PBMCs) of human were isolated and cultured for 10 hours,and then stimulated with 100 ng/ml LPS for 12 hours.Total RNA from these stimulated PBMCs were extracted,and cDNA of genes for human IL-23 p19 subunit were ampliphied by RT-PCR assay and inserted into pMD18-T vector.Results:The PCR production of human IL-23 p19 was verified by Electrophoresis proved to be 734 bp,result of sequencing was the same as the gene sequence of GenBank.Conclusions:cDNA encoding human interleukin-23 p19 was successfully cloned into cloning vector pMD18-T.
Objective:To clone genes for encoding human interleukin-23 (IL-23) p19 subunit.Methods:Peripheral blood mononuclear cells (PBMCs) of human were isolated and cultured for 10 hours,and then stimulated with 100 ng/ml LPS for 12 hours.Total RNA from these stimulated PBMCs were extracted,and cDNA of genes for human IL-23 p19 subunit were ampliphied by RT-PCR assay and inserted into pMD18-T vector.Results:The PCR production of human IL-23 p19 was verified by Electrophoresis proved to be 734 bp,result of sequencing was the same as the gene sequence of GenBank.Conclusions:cDNA encoding human interleukin-23 p19 was successfully cloned into cloning vector pMD18-T.
2010, 35(6): 548-551.
Abstract:
Objective:To investigate the influence of soy isoflavones on gene expression of myocardial sarcoplasmic reticulum calcium regulatory proteins in diabetic rats.Methods:Forty male Sprague-Dawley rats were randomly divided into four groups:normal control (NC) group,diabetic control (DC) group,soy isoflavones treatment (ST) group and nilestriol treatment (NT) group.All the groups were given streptozotocin (STZ) 55 mg/kg intraperitoneal injection (IP) except NC group.After 6 weeks,soy isoflavones 120 ml·kg-1·d-1 was given to ST group,nilestrioli 0.2 mg/kg to NT group twice a week,and the same CMC-Na was given to NC and DC groups.The volumes to all groups were 2 ml·kg-1·d-1.The drugs were all intragastrically administered using stomach tubes.The left ventricular systolic pressure (LVSP),left ventricular end diastole pressure (LVEDP) and Mean,±dp/dtmax of the rats were recorded at the end of the 12th week.The gene expressions of calcium regulatory proteins were detected by RTPCR.Results:Compared with NC group,the LVSP,LVEDP and Mean,±dp/dtmax of DC group were significantly lower (P<0.01),but those in ST and NT groups were markedly higher than DC group (P<0.01).LVEDP was increased in DC group while it was significantly decreased in ST and NT groups (P<0.01).The mRNA level of sarcoplasmic reticulum Ca2+-ATPase (SERCA),ryanodine receptor type 2 (RyR2) and 1,4,5-triphosphate inositor type 2 (IP3R2) in DC group were sharply decreased compared with that in NC group,but the mRNA level of phospholamban (PLB) was increased (P<0.01).The mRNA's expression of SERCA,RyR2,IP3R2 in ST and NT groups were significantly increased compared with that in DC group,but that of PLB was decreased (P<0.01).Conclusions:Soy isoflavones treatment could improve the heart function of the diabetic rats,which is possibly owing to its up-regulation of the levels of SERCA,RyR2,IP3R2 mRNAs and down-regulation of PLB mRNA.
Objective:To investigate the influence of soy isoflavones on gene expression of myocardial sarcoplasmic reticulum calcium regulatory proteins in diabetic rats.Methods:Forty male Sprague-Dawley rats were randomly divided into four groups:normal control (NC) group,diabetic control (DC) group,soy isoflavones treatment (ST) group and nilestriol treatment (NT) group.All the groups were given streptozotocin (STZ) 55 mg/kg intraperitoneal injection (IP) except NC group.After 6 weeks,soy isoflavones 120 ml·kg-1·d-1 was given to ST group,nilestrioli 0.2 mg/kg to NT group twice a week,and the same CMC-Na was given to NC and DC groups.The volumes to all groups were 2 ml·kg-1·d-1.The drugs were all intragastrically administered using stomach tubes.The left ventricular systolic pressure (LVSP),left ventricular end diastole pressure (LVEDP) and Mean,±dp/dtmax of the rats were recorded at the end of the 12th week.The gene expressions of calcium regulatory proteins were detected by RTPCR.Results:Compared with NC group,the LVSP,LVEDP and Mean,±dp/dtmax of DC group were significantly lower (P<0.01),but those in ST and NT groups were markedly higher than DC group (P<0.01).LVEDP was increased in DC group while it was significantly decreased in ST and NT groups (P<0.01).The mRNA level of sarcoplasmic reticulum Ca2+-ATPase (SERCA),ryanodine receptor type 2 (RyR2) and 1,4,5-triphosphate inositor type 2 (IP3R2) in DC group were sharply decreased compared with that in NC group,but the mRNA level of phospholamban (PLB) was increased (P<0.01).The mRNA's expression of SERCA,RyR2,IP3R2 in ST and NT groups were significantly increased compared with that in DC group,but that of PLB was decreased (P<0.01).Conclusions:Soy isoflavones treatment could improve the heart function of the diabetic rats,which is possibly owing to its up-regulation of the levels of SERCA,RyR2,IP3R2 mRNAs and down-regulation of PLB mRNA.
2010, 35(6): 552-554.
Abstract:
Objective:To study the cleavage effect of M1 RNA,catalytic subunit of RNase P from Escherichia colion on UL54 mRNA encoding major DNA polymerase of human cytomegalovirus in vitro,and to explore the prospects of ribozyme as a novel antiviral agent.Methods:An external guiding sequence T6 specific to HCMV UL54 mRNA was designed and synthesized;specific cleavage activity of M1RNA guided by T6 was observed in vitro.Results:With the guidance of EGS-T6,M1 RNA from Escherichia coli can block the expression of HCMV UL54 mRNA in vitro.Conclusions:EGS-T6 may fulfill the specific cleavage and be developed as a novel antiviral agent.
Objective:To study the cleavage effect of M1 RNA,catalytic subunit of RNase P from Escherichia colion on UL54 mRNA encoding major DNA polymerase of human cytomegalovirus in vitro,and to explore the prospects of ribozyme as a novel antiviral agent.Methods:An external guiding sequence T6 specific to HCMV UL54 mRNA was designed and synthesized;specific cleavage activity of M1RNA guided by T6 was observed in vitro.Results:With the guidance of EGS-T6,M1 RNA from Escherichia coli can block the expression of HCMV UL54 mRNA in vitro.Conclusions:EGS-T6 may fulfill the specific cleavage and be developed as a novel antiviral agent.
2010, 35(6): 555-557,561.
Abstract:
Objective:To study the effect of down-regulating glucose regulated protein 78 (GRP 78) on adriamycin (ADR) induced apoptosis in human breast cancer cell line SK-BR-3,and to provide a new target for chemotherapy of breast cancer.Methods:Human breast cancer cell line SK-BR-3 cells were cultured,and treated with ADR (1 mg/L) for 0,6,12,24,and 36 hours;the expression of GRP78 at different time points was measured by Western blot.After treated with ADR for 48 hours,SK-BR-3 cells were harvest and stained with propidium iodide for apoptotic assay.SK-BR-3 cells were pretreated with siRNA,and the same was done to ADR;cell apoptosis was measured to compare the effect of GRP78 on ADR induced apoptosis in SK-BR-3.Results:ADR induced ER stress and up-regulated the expression of GRP78.ADR induced less than 30% cell apoptosis in SK-BR-3 cells.siRNA significantly blocked the expression of GRP78 and increased the apoptosis induced by ADR.The apoptosis rate was (62.30±0.88)% (P<0.01).Conclusions:Inhibition of the expression of GRP78 in human breast cancer cells SK-BR-3 can increase the sensitivity of ADR-induced apoptosis through ER pathway,which may promote the apoptosis of tumor cells and enhance the anti-tumor effect.
Objective:To study the effect of down-regulating glucose regulated protein 78 (GRP 78) on adriamycin (ADR) induced apoptosis in human breast cancer cell line SK-BR-3,and to provide a new target for chemotherapy of breast cancer.Methods:Human breast cancer cell line SK-BR-3 cells were cultured,and treated with ADR (1 mg/L) for 0,6,12,24,and 36 hours;the expression of GRP78 at different time points was measured by Western blot.After treated with ADR for 48 hours,SK-BR-3 cells were harvest and stained with propidium iodide for apoptotic assay.SK-BR-3 cells were pretreated with siRNA,and the same was done to ADR;cell apoptosis was measured to compare the effect of GRP78 on ADR induced apoptosis in SK-BR-3.Results:ADR induced ER stress and up-regulated the expression of GRP78.ADR induced less than 30% cell apoptosis in SK-BR-3 cells.siRNA significantly blocked the expression of GRP78 and increased the apoptosis induced by ADR.The apoptosis rate was (62.30±0.88)% (P<0.01).Conclusions:Inhibition of the expression of GRP78 in human breast cancer cells SK-BR-3 can increase the sensitivity of ADR-induced apoptosis through ER pathway,which may promote the apoptosis of tumor cells and enhance the anti-tumor effect.
2010, 35(6): 562-563,567.
Abstract:
Objective:To assess the effect of 131I therapy on patients with hyperthyroidism complicated with hepatic dysfunction.Methods:Clinical data of 71 cases of hyperthyroidism with liver damage were studied retrospectively.Results:Serum levels of FT3,FT4,aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,total bilirubin were obviously improved after 131I treatment (P<0.05 to P<0.01).Of the 71 cases,the liver function of 61 turned normal and 10 showed significant efficacy after 6 months,5 presented with hypothyroidism.Conclusions:131I is safe and effective in treating hyperthyroidism complicated with hepatic dysfunction.
Objective:To assess the effect of 131I therapy on patients with hyperthyroidism complicated with hepatic dysfunction.Methods:Clinical data of 71 cases of hyperthyroidism with liver damage were studied retrospectively.Results:Serum levels of FT3,FT4,aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,total bilirubin were obviously improved after 131I treatment (P<0.05 to P<0.01).Of the 71 cases,the liver function of 61 turned normal and 10 showed significant efficacy after 6 months,5 presented with hypothyroidism.Conclusions:131I is safe and effective in treating hyperthyroidism complicated with hepatic dysfunction.
2010, 35(6): 564-567.
Abstract:
Objective:To determine the clinical,pathological and radiological features of hypertrophic cranial pachymeningitis (HCP).Methods:The clinical data of 5 patients with HCP were studied retrospectively,and the literature was reviewed.Results:All the 5 patients presented the symptom of headache.Two of them were accompanied by language barriers,1 by limb convulsion,reaction slowness,cranial nerve damage and walking instability,and 1 by memory impairment.MRI scan showed that 2 cases displayed edema changes within the brain parenchyma,and there was a wide range of enhanced thickening in all the 5 cases after enhancement,particularly at the frontal and top of the dura mater,the falx and the tentorium.The brain biopsy of 1 patient showed that the dura mater was significantly thickened,and the dural fibrosis pathology indicated the existence of a large number of plasma cell,lymphocyte,macrophage,neutrophil infiltration and focal calcification,which was considered to be a non-specific chronic inflammation.The brain blood vessels under the dura mater were dilated,with plasma cell and macrophage infiltration.The corresponding brain parenchyma also displayed non-specific inflammatory changes.Conclusions:Causes of HCP are complicated.The common clinical manifestations include headache,body convulsion,reaction slowness,speech impairment,memory impairment,walking instability and cranial nerve damage;Pathology showed dural fibrosis and inflammatory cell infiltration;the corresponding parts of the brain parenchyma may also display non-specific inflammatory changes,and such typical changes in head MRI enhanced imaging is beneficial to clinical diagnosis.
Objective:To determine the clinical,pathological and radiological features of hypertrophic cranial pachymeningitis (HCP).Methods:The clinical data of 5 patients with HCP were studied retrospectively,and the literature was reviewed.Results:All the 5 patients presented the symptom of headache.Two of them were accompanied by language barriers,1 by limb convulsion,reaction slowness,cranial nerve damage and walking instability,and 1 by memory impairment.MRI scan showed that 2 cases displayed edema changes within the brain parenchyma,and there was a wide range of enhanced thickening in all the 5 cases after enhancement,particularly at the frontal and top of the dura mater,the falx and the tentorium.The brain biopsy of 1 patient showed that the dura mater was significantly thickened,and the dural fibrosis pathology indicated the existence of a large number of plasma cell,lymphocyte,macrophage,neutrophil infiltration and focal calcification,which was considered to be a non-specific chronic inflammation.The brain blood vessels under the dura mater were dilated,with plasma cell and macrophage infiltration.The corresponding brain parenchyma also displayed non-specific inflammatory changes.Conclusions:Causes of HCP are complicated.The common clinical manifestations include headache,body convulsion,reaction slowness,speech impairment,memory impairment,walking instability and cranial nerve damage;Pathology showed dural fibrosis and inflammatory cell infiltration;the corresponding parts of the brain parenchyma may also display non-specific inflammatory changes,and such typical changes in head MRI enhanced imaging is beneficial to clinical diagnosis.
2010, 35(6): 568-570.
Abstract:
Objective:To assess the value of heart-type fatty acid binding protein (H-FABP) in early diagnosis of acute myocardial infarction (AMI).Methods:Sixty cases of AMI patients were divided into 2 groups according to the time of receiving treatment.The H-FABP,cTnI and CK-MB were detected 0-3 hours and 4-6 hours after the onset.The sensitivity,specificity,positive predictive value and negative predictive value were calculated,and the results were analyzed and compared with those of the normal control and the angor pectoris group.Results:The level of plasma H-FABP concentration in the AMI patients receiving treatment 0-3 hours and 4-6 hours after onset was significantly higher than that of the the control group and the angor pectoris group (P<0.01);the sensitivity of H-FABP within 0-3 hours and 4-6 hours of onset was 67.74% and 89.66%,respectively in diagnosis of AMI,which was significantly higher than that of cTnI (29.03%,62.07%) and CK-MB (0.00%,58.62%) (P<0.01 and P<0.05);the positive predictive value and negative predictive value of H-FABP were 91.30% and 86.84%,CK-MB within 0-3 hours of onset were 0.00% and 68.69%.The specificity value of H-FABP within 0-3 hours and 4-6 hours of onset was 97.06%,which was similar to that of cTnI and CK-MB in diagnosis of AMI were 98.53% and 100.00%.Conclusions:H-FABP is superior to cTnI and CK-MB in diagnosis of AMI.
Objective:To assess the value of heart-type fatty acid binding protein (H-FABP) in early diagnosis of acute myocardial infarction (AMI).Methods:Sixty cases of AMI patients were divided into 2 groups according to the time of receiving treatment.The H-FABP,cTnI and CK-MB were detected 0-3 hours and 4-6 hours after the onset.The sensitivity,specificity,positive predictive value and negative predictive value were calculated,and the results were analyzed and compared with those of the normal control and the angor pectoris group.Results:The level of plasma H-FABP concentration in the AMI patients receiving treatment 0-3 hours and 4-6 hours after onset was significantly higher than that of the the control group and the angor pectoris group (P<0.01);the sensitivity of H-FABP within 0-3 hours and 4-6 hours of onset was 67.74% and 89.66%,respectively in diagnosis of AMI,which was significantly higher than that of cTnI (29.03%,62.07%) and CK-MB (0.00%,58.62%) (P<0.01 and P<0.05);the positive predictive value and negative predictive value of H-FABP were 91.30% and 86.84%,CK-MB within 0-3 hours of onset were 0.00% and 68.69%.The specificity value of H-FABP within 0-3 hours and 4-6 hours of onset was 97.06%,which was similar to that of cTnI and CK-MB in diagnosis of AMI were 98.53% and 100.00%.Conclusions:H-FABP is superior to cTnI and CK-MB in diagnosis of AMI.
2010, 35(6): 571-572,575.
Abstract:
Objective:To investigate the role of perflurocarbon liquids (PFCLs) in vitrectomy for complicated vitroretinopathy.Methods:Eighteen cases (18 eyes) of complicated proliferative vitroretinopathy were treated by vitrectomy.A small quantity of PFCLs was used during and after retinal membrane peeling,retinal funnels opening and posterior retina stabilization.Edolaser photocoagulation was performed and C3F8 or silicon oil was filled.Visual acuity and retinal reattachment condition were examined after the operation.Results:The rate of retinal reattachment reached 77.8%.The final visual acuity of 10 cases reached 0.05 or above.The effect was obvious (P<0.01).A small amount of PFLCs retained in 2 cases (11.1%) and secondary glaucoma occurred in 1 case.Conclusions:As a hydrodynamic operative tool,PFCLs can be used in vitrectomy for complicated vitroretinopathy to facilitate the operation and improve the outcome.
Objective:To investigate the role of perflurocarbon liquids (PFCLs) in vitrectomy for complicated vitroretinopathy.Methods:Eighteen cases (18 eyes) of complicated proliferative vitroretinopathy were treated by vitrectomy.A small quantity of PFCLs was used during and after retinal membrane peeling,retinal funnels opening and posterior retina stabilization.Edolaser photocoagulation was performed and C3F8 or silicon oil was filled.Visual acuity and retinal reattachment condition were examined after the operation.Results:The rate of retinal reattachment reached 77.8%.The final visual acuity of 10 cases reached 0.05 or above.The effect was obvious (P<0.01).A small amount of PFLCs retained in 2 cases (11.1%) and secondary glaucoma occurred in 1 case.Conclusions:As a hydrodynamic operative tool,PFCLs can be used in vitrectomy for complicated vitroretinopathy to facilitate the operation and improve the outcome.
2010, 35(6): 573-575.
Abstract:
Objective:To assess the value of intracerebral hemorrhage (ICH) score in clinical practice.Methods:Fifty-three cases of spontaneous ICH patients were evaluated by ICH score and clinical neurologic deficit scale (CNDS) at admission.The association of ICH score with 30-day mortality and neurologic deficit scale was analyzed.Results:The 30-day mortality in patients with ICH score ≥ 3 were higher than those with ICH score<3 (P<0.01).The ICH score was positively correlated with CNDS (P<0.01).Conclusions:ICH score is a simple and reliable method which can accurately predict the clinical prognosis of patients with spontaneous intracerebral hemorrhage.
Objective:To assess the value of intracerebral hemorrhage (ICH) score in clinical practice.Methods:Fifty-three cases of spontaneous ICH patients were evaluated by ICH score and clinical neurologic deficit scale (CNDS) at admission.The association of ICH score with 30-day mortality and neurologic deficit scale was analyzed.Results:The 30-day mortality in patients with ICH score ≥ 3 were higher than those with ICH score<3 (P<0.01).The ICH score was positively correlated with CNDS (P<0.01).Conclusions:ICH score is a simple and reliable method which can accurately predict the clinical prognosis of patients with spontaneous intracerebral hemorrhage.
2010, 35(6): 576-578.
Abstract:
Objective:To evaluate ultrasound guided aspiration biopsy (USGAB) in diagnosis of splenic space-occupying lesion.Methods:Twenty-two patients with or combined with metastatic carcinoma of the spleen were detected by USGAB,and the tissues underwent pathologic examination.Results:Twenty cases were punctured once,and the rest twice.The average needle pass was (2.28±0.46) times,and the average length of the core organization was (14.7±1.3) mm.The puncture success rate was 100%,and the pathological diagnosis rate was 100%.Among the 22 cases,17 were malignant lesions and 5 benign lesions.No serious complications occurred.Conclusions:USGAB technology is safe,accurate and rapid in pathological diagnosis of splenic space-occupying lesion.It is an effective diagnostic method.
Objective:To evaluate ultrasound guided aspiration biopsy (USGAB) in diagnosis of splenic space-occupying lesion.Methods:Twenty-two patients with or combined with metastatic carcinoma of the spleen were detected by USGAB,and the tissues underwent pathologic examination.Results:Twenty cases were punctured once,and the rest twice.The average needle pass was (2.28±0.46) times,and the average length of the core organization was (14.7±1.3) mm.The puncture success rate was 100%,and the pathological diagnosis rate was 100%.Among the 22 cases,17 were malignant lesions and 5 benign lesions.No serious complications occurred.Conclusions:USGAB technology is safe,accurate and rapid in pathological diagnosis of splenic space-occupying lesion.It is an effective diagnostic method.
2010, 35(6): 579-580.
Abstract:
Objective:To explore an effective therapy for elderly patients with colon cancer complicated with acute intestinal obstruction.Methods:Thirty-seven aged patients with colon cancer complicated with acute intestinal obstruction underwent emergency operation.Twenty-two of the cases were performed one-stage resection and anastomosis,8 cases one-stage resection and anastomosis with proximal colostomy and 5 cases proximal colostomy as well as two-stage resection and anastomosis.Hartmann was observed in 2 cases.Results:All the 37 cases were cured and no preoperative mortality occurred.Postoperative anastomotic leakage was found in 1 case,incision infection in 3 cases,pulmonary infection in 1 case and abdominal infection in 1 case.Conclusions:Early diagnosis,proper perioperative management and cognition of operation indications are essential for treatment of colon cancer complicated with acute intestinal obstruction by one-stage resection and anastomosis.
Objective:To explore an effective therapy for elderly patients with colon cancer complicated with acute intestinal obstruction.Methods:Thirty-seven aged patients with colon cancer complicated with acute intestinal obstruction underwent emergency operation.Twenty-two of the cases were performed one-stage resection and anastomosis,8 cases one-stage resection and anastomosis with proximal colostomy and 5 cases proximal colostomy as well as two-stage resection and anastomosis.Hartmann was observed in 2 cases.Results:All the 37 cases were cured and no preoperative mortality occurred.Postoperative anastomotic leakage was found in 1 case,incision infection in 3 cases,pulmonary infection in 1 case and abdominal infection in 1 case.Conclusions:Early diagnosis,proper perioperative management and cognition of operation indications are essential for treatment of colon cancer complicated with acute intestinal obstruction by one-stage resection and anastomosis.
2010, 35(6): 581-582,584.
Abstract:
Objective:To investigate the expression of nuclear factor κB (NF-κb) and vascular endothelial growth factor (VEGF) in retinoblastoma (RB),and its relationship with the differentiation and optic nerve infiltration.Methods:The expression of NF-κB and VEGF was examined in 33 cases of RB tissue using immunohistochemistry.The relationship between the differentiation and invasion among NF-κB,VEGF and RB was analyzed statistically.Results:The expression of RB in NF-κB and VEGF was obviously higher than that in normal retina (P<0.01),and the expression was related to the differentiation degree of RB (P=0.027).The expression of VEGF was significantly different between invasive and non-invasive optic nerves of RB (P=0.033).Conclusions:NF-κB and VEGF are both involved in the formation of RB,and VEGF may be associated with the invasion of RB.
Objective:To investigate the expression of nuclear factor κB (NF-κb) and vascular endothelial growth factor (VEGF) in retinoblastoma (RB),and its relationship with the differentiation and optic nerve infiltration.Methods:The expression of NF-κB and VEGF was examined in 33 cases of RB tissue using immunohistochemistry.The relationship between the differentiation and invasion among NF-κB,VEGF and RB was analyzed statistically.Results:The expression of RB in NF-κB and VEGF was obviously higher than that in normal retina (P<0.01),and the expression was related to the differentiation degree of RB (P=0.027).The expression of VEGF was significantly different between invasive and non-invasive optic nerves of RB (P=0.033).Conclusions:NF-κB and VEGF are both involved in the formation of RB,and VEGF may be associated with the invasion of RB.
2010, 35(6): 583-584.
Abstract:
Objective:To study the effect of standard decompressive craniotomy on severe traumatic brain injury.Methods:The clinical data of 60 cases of severe traumatic brain injury treated by standard decompressive craniectomy were retrospectively reviewed.Results:All the cases were followed up for six months to one year.Good recovery was observed in 8 cases,moderate deficit in 10 cases,severe deficit in 11 cases,persistent vegetative status in 12 and death in 19 cases.Conclusions:Standard decompressive craniotomy improves the outcome of severe traumatic brain injury.
Objective:To study the effect of standard decompressive craniotomy on severe traumatic brain injury.Methods:The clinical data of 60 cases of severe traumatic brain injury treated by standard decompressive craniectomy were retrospectively reviewed.Results:All the cases were followed up for six months to one year.Good recovery was observed in 8 cases,moderate deficit in 10 cases,severe deficit in 11 cases,persistent vegetative status in 12 and death in 19 cases.Conclusions:Standard decompressive craniotomy improves the outcome of severe traumatic brain injury.
2010, 35(6): 585-586.
Abstract:
Objective:To observe the value of determining the serum levels and C-reactive protein (CRP) in diagnosis and prognosis of acute pancreatitis (AP).Methods:According to the results of Ranson score and CT grades,33 patients were classified into mild acute pancreatitis group (15 cases),severe acute pancreatitis group (18 cases),SAP with complication group (4 cases) and SAP without complication group (14 cases).The Serum levels of all the patients were determined with full automatic biochemistry analyzer on day 1,3,7 and 14 of admission,and 12 healthy people acted as control.Results:The CRP in AP patients was significantly higher than that of the normal control at admission (P<0.01).The CRP was significantly higher in the severe AP group than in the mild AP group,and the CRP in SAP with complication group was significantly higher than that without complication group one week after admission (P<0.01).The serum value of CRP in the SAP groups reached the peak on the third day after onset.Conclusions:Dynamic changes of the serum levels of CRP are related to the occurrence and development of AP;the serum levels of CRP may act as a predictive index in diagnosis and prognosis of acute pancreatitis.
Objective:To observe the value of determining the serum levels and C-reactive protein (CRP) in diagnosis and prognosis of acute pancreatitis (AP).Methods:According to the results of Ranson score and CT grades,33 patients were classified into mild acute pancreatitis group (15 cases),severe acute pancreatitis group (18 cases),SAP with complication group (4 cases) and SAP without complication group (14 cases).The Serum levels of all the patients were determined with full automatic biochemistry analyzer on day 1,3,7 and 14 of admission,and 12 healthy people acted as control.Results:The CRP in AP patients was significantly higher than that of the normal control at admission (P<0.01).The CRP was significantly higher in the severe AP group than in the mild AP group,and the CRP in SAP with complication group was significantly higher than that without complication group one week after admission (P<0.01).The serum value of CRP in the SAP groups reached the peak on the third day after onset.Conclusions:Dynamic changes of the serum levels of CRP are related to the occurrence and development of AP;the serum levels of CRP may act as a predictive index in diagnosis and prognosis of acute pancreatitis.
2010, 35(6): 587-588.
Abstract:
Objective:To evaluate hollow screw fixation with small incision in treatment of calcaneal intra-articular fractures.Methods:Twenty-five feet with calcaneal fractures were fixed by hollow screws with small incisions (4-6 cm) at the lateral side of the calcaneu.The changes of Bölher's angles of the calcaneus and the width of the calcaneus were observed before and after the operation.Results:The Bölher's angles and the width of the calcaneus changed significantly before and after the operation (P<0.01);the incision of all the cases healed at one stage with no infection;the follow-up lasted from 4 to 24 months.The effect was evaluated by Fernandez score standard,and the results were excellent in 16 cases,good in 7 and fair in 2.The excellent and good rates reached 92.0%.Conclusions:The procedure of hollow screw fixation with limited open reduction is safe and effective for treatment of calcaneal intra-articular fractures.
Objective:To evaluate hollow screw fixation with small incision in treatment of calcaneal intra-articular fractures.Methods:Twenty-five feet with calcaneal fractures were fixed by hollow screws with small incisions (4-6 cm) at the lateral side of the calcaneu.The changes of Bölher's angles of the calcaneus and the width of the calcaneus were observed before and after the operation.Results:The Bölher's angles and the width of the calcaneus changed significantly before and after the operation (P<0.01);the incision of all the cases healed at one stage with no infection;the follow-up lasted from 4 to 24 months.The effect was evaluated by Fernandez score standard,and the results were excellent in 16 cases,good in 7 and fair in 2.The excellent and good rates reached 92.0%.Conclusions:The procedure of hollow screw fixation with limited open reduction is safe and effective for treatment of calcaneal intra-articular fractures.
2010, 35(6): 589-590,592.
Abstract:
Objective:To investigate the therapeutic efficacy of ondansetron combined with metoclopramide and vitamin B6 on gastrointestinal reaction induced by chemotherapy of acute leukaemia.Methods:The patients with acute myeloid leukemia were intravenously injected 8 mg ondansetron and 100 ml of 5% GS 30 min before chemotherapy,and 20-30 mg metoclopramide,100-200 mg vitamin B6 and 100 ml of 5% GS 4 hours after chemotherapy for 5 days.The patients with acute lymphoid leukemia were given ondansetron 30 min before chemotherapy at the same dosage as the patients with acute myeloid leukemia.The patients in control group were given 8 mg ondansetron and 100 ml of 5% GS 30 min before chemotherapy and 4 hours after chemotherapy.Results:The degree of nausea and antiemetic effect had no statistical difference between the two groups (P>0.05).Conclusions:The combined therapy of ondansetron,metoclopramide and vitamin B6 has similar effect to repeated use of ondansetron on gastrointestinal reaction induced by chemotherapy of acute leukaemia.
Objective:To investigate the therapeutic efficacy of ondansetron combined with metoclopramide and vitamin B6 on gastrointestinal reaction induced by chemotherapy of acute leukaemia.Methods:The patients with acute myeloid leukemia were intravenously injected 8 mg ondansetron and 100 ml of 5% GS 30 min before chemotherapy,and 20-30 mg metoclopramide,100-200 mg vitamin B6 and 100 ml of 5% GS 4 hours after chemotherapy for 5 days.The patients with acute lymphoid leukemia were given ondansetron 30 min before chemotherapy at the same dosage as the patients with acute myeloid leukemia.The patients in control group were given 8 mg ondansetron and 100 ml of 5% GS 30 min before chemotherapy and 4 hours after chemotherapy.Results:The degree of nausea and antiemetic effect had no statistical difference between the two groups (P>0.05).Conclusions:The combined therapy of ondansetron,metoclopramide and vitamin B6 has similar effect to repeated use of ondansetron on gastrointestinal reaction induced by chemotherapy of acute leukaemia.
2010, 35(6): 591-592.
Abstract:
Objective:To explore the changes and clinical significance of interleukin-12 (IL-12) in the peritoneal fluid and serum of patients with endometriosis.Methods:IL-12 in the peritoneal fluid and serum of 39 patients with endometriosis and 16 controls were detected by enzyme linked immunosorbent assay (ELISA).Results:IL-12 levels in the peritoneal fluid and serum of the endometriosis group were lower than those of the control group (P<0.01).IL-12 levels in the peritoneal fluid[(62.37±13.7) ng/L] and in the serum[(53.64±17.4) ng/L] of patients with stageⅢ-Ⅳ endometriosis were lower than those in the peritoneal fluid[(76.38±13.5) ng/L]and in the serum[(75.67±12.3) ng/L] of patients with stageⅠ-Ⅱendometriosis (P<0.01).Conclusions:IL-12 may play an important role in the pathogenesis of endometriosis.Determining the IL-12 levels in patients with endometriosis could help to the diagnosis and treatment.
Objective:To explore the changes and clinical significance of interleukin-12 (IL-12) in the peritoneal fluid and serum of patients with endometriosis.Methods:IL-12 in the peritoneal fluid and serum of 39 patients with endometriosis and 16 controls were detected by enzyme linked immunosorbent assay (ELISA).Results:IL-12 levels in the peritoneal fluid and serum of the endometriosis group were lower than those of the control group (P<0.01).IL-12 levels in the peritoneal fluid[(62.37±13.7) ng/L] and in the serum[(53.64±17.4) ng/L] of patients with stageⅢ-Ⅳ endometriosis were lower than those in the peritoneal fluid[(76.38±13.5) ng/L]and in the serum[(75.67±12.3) ng/L] of patients with stageⅠ-Ⅱendometriosis (P<0.01).Conclusions:IL-12 may play an important role in the pathogenesis of endometriosis.Determining the IL-12 levels in patients with endometriosis could help to the diagnosis and treatment.
2010, 35(6): 593-594,596.
Abstract:
Objective:To observe the effect and security of pre-emptive analgesia with tramadol in patients undergoing anorectal surgery by total intravenous anesthesia.Methods:Sixty patients in physical status Ⅰ-Ⅱas scored by American Society of Anesthesiologists (ASA) and undergoing anorectal surgery were randomly divided into two groups.Group A were induced with fentanyl 3 μg/kg and propofol 2-3 mg/kg,and were anesthetized with continuous infusion of propofol 6-10 mg·kg-1·h-1.Group B received an intravenous injection of tramadol 1.5-2 mg/kg 3 min before induction,and then were induced with fentanyl 1.5 μg/kg and propofol 2-3 mg/kg with the same maintenance as group A.The visual analogue scales (VAS) and side effects such as nausea and vomiting 6 hours after operation were observed.Results:The operation was successful in both groups.Nine patients in group A and one patient in group B resorted to drugs due to SpO2 decrease during anesthesia.The difference was significant (P<0.01).The VAS of group B was markedly lower and the effect of analgesia was better than that of group A 6 hours after operation (P<0.05-P<0.01).Conclusions:Pre-emptive analgesia with tramadol for patients undergoing anorectal surgery using total intravenous anesthesia can provide satisfactory analgesic effect and reduce the dosage of fentanyl to 1.5 μg/kg,which may decrease the influence of SpO2 and mean arterial pressure during anesthesia.This technique has certain clinical value.
Objective:To observe the effect and security of pre-emptive analgesia with tramadol in patients undergoing anorectal surgery by total intravenous anesthesia.Methods:Sixty patients in physical status Ⅰ-Ⅱas scored by American Society of Anesthesiologists (ASA) and undergoing anorectal surgery were randomly divided into two groups.Group A were induced with fentanyl 3 μg/kg and propofol 2-3 mg/kg,and were anesthetized with continuous infusion of propofol 6-10 mg·kg-1·h-1.Group B received an intravenous injection of tramadol 1.5-2 mg/kg 3 min before induction,and then were induced with fentanyl 1.5 μg/kg and propofol 2-3 mg/kg with the same maintenance as group A.The visual analogue scales (VAS) and side effects such as nausea and vomiting 6 hours after operation were observed.Results:The operation was successful in both groups.Nine patients in group A and one patient in group B resorted to drugs due to SpO2 decrease during anesthesia.The difference was significant (P<0.01).The VAS of group B was markedly lower and the effect of analgesia was better than that of group A 6 hours after operation (P<0.05-P<0.01).Conclusions:Pre-emptive analgesia with tramadol for patients undergoing anorectal surgery using total intravenous anesthesia can provide satisfactory analgesic effect and reduce the dosage of fentanyl to 1.5 μg/kg,which may decrease the influence of SpO2 and mean arterial pressure during anesthesia.This technique has certain clinical value.
2010, 35(6): 595-596.
Abstract:
Objective:To compare the clinical effects of harringtonine+arabinosylcytosin (HA) and aclarubicin+arabinosylcytosin+recombinant human granulocyte-colony stimulating factor (CAG) regimen in treatment of acute non-lymphocytic leukemia (ANLL) in the elderly.Methods:Thirty-two elderly patients with ANLL were randomly divided into HA group and CAG group,and the clinical efficacy was compared between the two groups.Results:The complete remission rate was 56.3% in the CAG group and 43.8% in the HA group.The total efficacy rates were 68.8% and 62.5%,respectively.The difference was not significant (P>0.05).Conclusions:CAG regimen is more suitable than HA regimen in treatment of ANLL in the elderly.It is worth popularizing.
Objective:To compare the clinical effects of harringtonine+arabinosylcytosin (HA) and aclarubicin+arabinosylcytosin+recombinant human granulocyte-colony stimulating factor (CAG) regimen in treatment of acute non-lymphocytic leukemia (ANLL) in the elderly.Methods:Thirty-two elderly patients with ANLL were randomly divided into HA group and CAG group,and the clinical efficacy was compared between the two groups.Results:The complete remission rate was 56.3% in the CAG group and 43.8% in the HA group.The total efficacy rates were 68.8% and 62.5%,respectively.The difference was not significant (P>0.05).Conclusions:CAG regimen is more suitable than HA regimen in treatment of ANLL in the elderly.It is worth popularizing.
2010, 35(6): 597-598,601.
Abstract:
Objective:To observe the curative effect of noninvasive positive pressure ventilation (NPPV) in treatment of acute cardiogenic pulmonary edema.Methods:Twenty-six patients with acute respiratory failure due to acute cardiogenic pulmonary edema were treated by NPPV.The variation of heart rate and percutaneous oxygen saturation were observed;the blood gas analysis was compared before treatment and 2 hours after treatment.Results:The symptoms of the patients were significantly alleviated including hypoxia,heart palpitations,breathing difficulties and cyanosis,and the NYHA decreased significantly.Conclusions:NPPV has therapeutic advantages and significant effect in treatment of acute respiratory failure due to acute cardiogenic pulmonary edema.
Objective:To observe the curative effect of noninvasive positive pressure ventilation (NPPV) in treatment of acute cardiogenic pulmonary edema.Methods:Twenty-six patients with acute respiratory failure due to acute cardiogenic pulmonary edema were treated by NPPV.The variation of heart rate and percutaneous oxygen saturation were observed;the blood gas analysis was compared before treatment and 2 hours after treatment.Results:The symptoms of the patients were significantly alleviated including hypoxia,heart palpitations,breathing difficulties and cyanosis,and the NYHA decreased significantly.Conclusions:NPPV has therapeutic advantages and significant effect in treatment of acute respiratory failure due to acute cardiogenic pulmonary edema.
2010, 35(6): 599-601.
Abstract:
Objective:To assess the clinical characteristics of elderly patients with first onset depression with psychotic symptoms.Methods:One hundred and nine elderly patients with first onset depression were divided into group A (with psychotic symptoms,n=15) and group B (without psychotic symptoms,n=94),and the clinical characteristics were compared between them.Results:The suicide rate in group A was 53.3%,which was significantly higher than that in group B (24.5%).The difference was significant (P<0.05).The suicidal behavior was significantly related to delusion (RR=3.53).The most common psychotic symptoms were delusions of guilty (40.0%),persecution (13.3%) and hypochondriasis (6.7%),and 40.0% of the patients were accompanied with auditory hallucinations.There were significant differences in treatment with antipsychotics and antidepressants between the two groups (P=0.018).No significant difference was observed in recent efficacy between the two groups (P>0.05).Conclusions:Suicidal behavior is more common in elderly depression patients with psychotic symptoms,and antidepressants combined with antipsychotics should be applied to those with psychotic symptoms.
Objective:To assess the clinical characteristics of elderly patients with first onset depression with psychotic symptoms.Methods:One hundred and nine elderly patients with first onset depression were divided into group A (with psychotic symptoms,n=15) and group B (without psychotic symptoms,n=94),and the clinical characteristics were compared between them.Results:The suicide rate in group A was 53.3%,which was significantly higher than that in group B (24.5%).The difference was significant (P<0.05).The suicidal behavior was significantly related to delusion (RR=3.53).The most common psychotic symptoms were delusions of guilty (40.0%),persecution (13.3%) and hypochondriasis (6.7%),and 40.0% of the patients were accompanied with auditory hallucinations.There were significant differences in treatment with antipsychotics and antidepressants between the two groups (P=0.018).No significant difference was observed in recent efficacy between the two groups (P>0.05).Conclusions:Suicidal behavior is more common in elderly depression patients with psychotic symptoms,and antidepressants combined with antipsychotics should be applied to those with psychotic symptoms.
2010, 35(6): 602-604.
Abstract:
Objective:To investigate the effect of cervical loop electrosurgical excision (LEEP) in treatment of cervical intraepithelial neoplasia (CIN).Methods:Sixty-two patients initially diagnosed as CIN by cervical cytology,colposcopy and cervical biopsy were treated by LEEP.The effects were determined according to the preoperative and postoperative pathological changes and the follow-up.Results:The results of pathological examination and colposcopy biopsy after LEEP were significantly different (P<0.01).The cure rate of LEEP surgery was 98.33%,and the cure rate for patients with CIN 1 and 2 reached 100%.No recurrence was found one year after the operation.Conclusions:LEEP can provide complete specimen for pathology examination.It is an ideal method for the diagnosis and treatment of CIN.
Objective:To investigate the effect of cervical loop electrosurgical excision (LEEP) in treatment of cervical intraepithelial neoplasia (CIN).Methods:Sixty-two patients initially diagnosed as CIN by cervical cytology,colposcopy and cervical biopsy were treated by LEEP.The effects were determined according to the preoperative and postoperative pathological changes and the follow-up.Results:The results of pathological examination and colposcopy biopsy after LEEP were significantly different (P<0.01).The cure rate of LEEP surgery was 98.33%,and the cure rate for patients with CIN 1 and 2 reached 100%.No recurrence was found one year after the operation.Conclusions:LEEP can provide complete specimen for pathology examination.It is an ideal method for the diagnosis and treatment of CIN.
2010, 35(6): 605-606.
Abstract:
Objective:To explore the role of duodenoscope in treatment of residual and recurrent extrahepatic bile duct stones after cholecystectomy and choledochotomy.Methods:The clinical data of 31 patients undergoing duodenoscopic treatment for residual and recurrent extrahepatic bile duct stones were analyzed retrospectively.Results:All the 31 cases were diagnosed by endoscopic retrograde cholangiopancreatography (ERCP) and treated by endosopic sphincterotomy (EST).Thirty out of 31 cases (96.8%) were cured.After operation,1 case was converted to open operation because of cholangitis,2 cases had slight bleeding and 4 cases got a transient increase in serum amylase.Conclusions:ERCP is of great value in diagnosis of recrudescences of commonbile duct stones and operational complications.EST is an effective means in non-operation treatment of extrahepatic bile duct stones.
Objective:To explore the role of duodenoscope in treatment of residual and recurrent extrahepatic bile duct stones after cholecystectomy and choledochotomy.Methods:The clinical data of 31 patients undergoing duodenoscopic treatment for residual and recurrent extrahepatic bile duct stones were analyzed retrospectively.Results:All the 31 cases were diagnosed by endoscopic retrograde cholangiopancreatography (ERCP) and treated by endosopic sphincterotomy (EST).Thirty out of 31 cases (96.8%) were cured.After operation,1 case was converted to open operation because of cholangitis,2 cases had slight bleeding and 4 cases got a transient increase in serum amylase.Conclusions:ERCP is of great value in diagnosis of recrudescences of commonbile duct stones and operational complications.EST is an effective means in non-operation treatment of extrahepatic bile duct stones.
2010, 35(6): 607-608.
Abstract:
Objective:To explore the diagnosis and treatment of nodular goiter.Methods:Seventy-three patients with nodular goiter were received B ultrasonic examination,pathological test and surgical treatment.Results:Eleven patients recurred,7 received the second operation,4 presented dysphagia and 5 developed hypoparathyroidism which was restored to normal by conservative therapy.Conclusions:Ultrasonograph should be routinely performed before operation,and CT is required for patients with large tumors,dysphagia or dyspnea.Subtotal thyroidectomy is suggested;the normal thyroid tissue should be reserved as much as possible,and the recurrent laryngeal nerve and parathyroid should be prevented from injury.
Objective:To explore the diagnosis and treatment of nodular goiter.Methods:Seventy-three patients with nodular goiter were received B ultrasonic examination,pathological test and surgical treatment.Results:Eleven patients recurred,7 received the second operation,4 presented dysphagia and 5 developed hypoparathyroidism which was restored to normal by conservative therapy.Conclusions:Ultrasonograph should be routinely performed before operation,and CT is required for patients with large tumors,dysphagia or dyspnea.Subtotal thyroidectomy is suggested;the normal thyroid tissue should be reserved as much as possible,and the recurrent laryngeal nerve and parathyroid should be prevented from injury.
2010, 35(6): 615-616.
Abstract:
Objective:To analyze the factors affecting hand hygiene and investigate current condition of hand hygiene of clinicians.Methods:Before physical examination or invasive operation,samples were collected from 66 clinicians,and recognition of hand hygiene was estimated through questionnaire survey of the 66 clinicians at the same time.Results:Of all the 66 samples,disqualified rate was 69.7% (46 samples) and qualified rate 30.3%;all the 66 questionnaires were collected,and it revealed that hand washing rate of aseptic operation was 27% and the one of physical examination was 11% before contacting patients;the one of no touching patients' blood or humor was 20% and the one of touching it was 100% after contacting patients.Conclusions:In order to cut off the approach associated with hand hygiene through which infections were transmitted,it's necessary to strengthen training on hand washing of clinicians,improve recognition of hand hygiene and the facilities of hand washing,and perfect surveillance as well as feedback.
Objective:To analyze the factors affecting hand hygiene and investigate current condition of hand hygiene of clinicians.Methods:Before physical examination or invasive operation,samples were collected from 66 clinicians,and recognition of hand hygiene was estimated through questionnaire survey of the 66 clinicians at the same time.Results:Of all the 66 samples,disqualified rate was 69.7% (46 samples) and qualified rate 30.3%;all the 66 questionnaires were collected,and it revealed that hand washing rate of aseptic operation was 27% and the one of physical examination was 11% before contacting patients;the one of no touching patients' blood or humor was 20% and the one of touching it was 100% after contacting patients.Conclusions:In order to cut off the approach associated with hand hygiene through which infections were transmitted,it's necessary to strengthen training on hand washing of clinicians,improve recognition of hand hygiene and the facilities of hand washing,and perfect surveillance as well as feedback.
2010, 35(6): 617-618,621.
Abstract:
Objective:To determine the formaldehyde concentration in the air of anatomy laboratories at medical colleges.Methods:Medium flow air samplers were used for sampling;high-performance liquid chromatography was adopted;acetonitrile solution with 2,4-dinitrophenyl hydrazine was used as the Absorption liquid,and aqueous acetonitrile as the mobile phase;C18 reversed-phase column was applied for the separation;ultraviolet and visible light purple outside-visible detector was measured at 365 nm wavelength.Results:According to the analysis results,the formaldehyde concentration in the air was as high as 2.8 mg/m3.Conclusions:The concentration of formaldehyde in the air of the anatomy laboratory in a certain medical college is 23.3 times higher than the national standard in public places.It is necessary to ameliorate the environment where teachers and students are working and studying.
Objective:To determine the formaldehyde concentration in the air of anatomy laboratories at medical colleges.Methods:Medium flow air samplers were used for sampling;high-performance liquid chromatography was adopted;acetonitrile solution with 2,4-dinitrophenyl hydrazine was used as the Absorption liquid,and aqueous acetonitrile as the mobile phase;C18 reversed-phase column was applied for the separation;ultraviolet and visible light purple outside-visible detector was measured at 365 nm wavelength.Results:According to the analysis results,the formaldehyde concentration in the air was as high as 2.8 mg/m3.Conclusions:The concentration of formaldehyde in the air of the anatomy laboratory in a certain medical college is 23.3 times higher than the national standard in public places.It is necessary to ameliorate the environment where teachers and students are working and studying.
2010, 35(6): 619-621.
Abstract:
Objective:To study the chest X-ray features of children with pulmonary complications due to hand-foot-mouth disease.Methods:The X-ray CR chest films of 64 children with hand-foot-mouth disease were analyzed.Results:Of the 64 cases,22 manifested normal pulmonary features,42 abnormal features and 37 demonstrated fuzzy lung veins,dot-like,patchy dense shadows among lung veins.One case revealed pulmonary lobe consolidation and 4 cases appeared frosted-glass-like change in the lung fields.Conclusions:Pulmonary complications due to hand-foot-mouth disease manifest outstanding abnormal features in the X-ray film,which can help to learn the degree of pulmonary complications and guide the clinical therapy.
Objective:To study the chest X-ray features of children with pulmonary complications due to hand-foot-mouth disease.Methods:The X-ray CR chest films of 64 children with hand-foot-mouth disease were analyzed.Results:Of the 64 cases,22 manifested normal pulmonary features,42 abnormal features and 37 demonstrated fuzzy lung veins,dot-like,patchy dense shadows among lung veins.One case revealed pulmonary lobe consolidation and 4 cases appeared frosted-glass-like change in the lung fields.Conclusions:Pulmonary complications due to hand-foot-mouth disease manifest outstanding abnormal features in the X-ray film,which can help to learn the degree of pulmonary complications and guide the clinical therapy.
2010, 35(6): 622-623,626.
Abstract:
Objective:To investigate the role of ultrasound guided percutaneous biopsy in diagnosis of greater omentum mass.Methods:The sonograms of greater omentum and the pathological results of the patients were analyzed.Results:The successful rate of biopsy was 100%.Forty-five of the 64 cases were proved to be benign greater omentum mass including 35 cases of tuberculosis,2 chronic infection,2 cystic lymphangioma,1 leiomyoma and 1 teratoma,4 cases presented no specific findings.Among the 19 cases of malignant greater omentum mass,leiomyosarcoma was found in 2 cases,liposarcoma in 1 case,malignant mesothelioma in 2 cases,and lymphoma in 1 case and metastasis tumor in 13 cases.Conclusions:Ultrasound guided percutaneous biopsy is of great help to the diagnosis of greater omentum mass,even when the ascites cytology is negative.
Objective:To investigate the role of ultrasound guided percutaneous biopsy in diagnosis of greater omentum mass.Methods:The sonograms of greater omentum and the pathological results of the patients were analyzed.Results:The successful rate of biopsy was 100%.Forty-five of the 64 cases were proved to be benign greater omentum mass including 35 cases of tuberculosis,2 chronic infection,2 cystic lymphangioma,1 leiomyoma and 1 teratoma,4 cases presented no specific findings.Among the 19 cases of malignant greater omentum mass,leiomyosarcoma was found in 2 cases,liposarcoma in 1 case,malignant mesothelioma in 2 cases,and lymphoma in 1 case and metastasis tumor in 13 cases.Conclusions:Ultrasound guided percutaneous biopsy is of great help to the diagnosis of greater omentum mass,even when the ascites cytology is negative.
2010, 35(6): 624-626.
Abstract:
Objective:To investigate the clinical distribution,production and drug resistance of β-lactamase in nosocomial infection.Methods:The distribution and the drug resistance of 108 strains of Acinetobacter baumanii causing nosocomial infection were analyzed retrospectively.β-lactamase,cephalosporinase and extended spectrum β-lactamases (ESBLs) were detected by Nitrocefin test,3-dimension test and disc agar diffusion method,respectively.Results:The detection rates of Acinetobacter baumanii in ICU wards and neurosurgery wards were 63.89% and 11.11%,respectively.Acinetobacter baumanii was detected in 77.78% of the sputum samples and 12.96% of the secretion samples.The bacteria were resistant to most common antibiotics,and the rate of multiple-resistance was 88.89%.Eleven ESBLs bacteria and 78 cephalosporinase bacteria were detected in the 108 strains of Acinetobacter baumanii,accounting for 10.19% and 72.22%,respectively,and the positive rate of β-lactamase bacteria detected was 100.00%.Conclusions:Acinetobacter baumannii are the major opportunistic pathogenic bacteria in hospital infection.They are highly resistant to routine antimicrobial agents and even multi-drug resistant.The strains producing β-lactamase are the prominent cause of multi-drug resistance.Antimicrobial agents should be used rationally to decrease multi-drug resistance.
Objective:To investigate the clinical distribution,production and drug resistance of β-lactamase in nosocomial infection.Methods:The distribution and the drug resistance of 108 strains of Acinetobacter baumanii causing nosocomial infection were analyzed retrospectively.β-lactamase,cephalosporinase and extended spectrum β-lactamases (ESBLs) were detected by Nitrocefin test,3-dimension test and disc agar diffusion method,respectively.Results:The detection rates of Acinetobacter baumanii in ICU wards and neurosurgery wards were 63.89% and 11.11%,respectively.Acinetobacter baumanii was detected in 77.78% of the sputum samples and 12.96% of the secretion samples.The bacteria were resistant to most common antibiotics,and the rate of multiple-resistance was 88.89%.Eleven ESBLs bacteria and 78 cephalosporinase bacteria were detected in the 108 strains of Acinetobacter baumanii,accounting for 10.19% and 72.22%,respectively,and the positive rate of β-lactamase bacteria detected was 100.00%.Conclusions:Acinetobacter baumannii are the major opportunistic pathogenic bacteria in hospital infection.They are highly resistant to routine antimicrobial agents and even multi-drug resistant.The strains producing β-lactamase are the prominent cause of multi-drug resistance.Antimicrobial agents should be used rationally to decrease multi-drug resistance.
2010, 35(6): 627-629.
Abstract:
Objective:To compare the difference of using HBsAg or HBeAg as screening index of HBV infection during operation.Methods:HBsAg and HBeAg were used as supervising and controlling indexes of HBV infection during operation,and the difference was compared in three aspects:the infective source,the spreading route and the susceptible person.Results:HBV infective threshold value of single layer gloves was 105 ID/ml.The ratio of patients with HBsAg positive,HBeAg negative and HBeAg positive,whose infectivity had reached HBV infective threshold value,were 19.23%,3.20% and 83.88%,respectively.The difference was significant (P<0.01).After healthcare workers were inoculated against HBV,the HBV infective threshold value was 108.5 ID/ml during surgical operation;0.64% of the infectious HBsAg positive carriers exceeded the infective threshold value.Conclusions:HBsAg and HBeAg are greatly different as supervising and controlling indexes of HBV infection in infective source,spread route and susceptible person.
Objective:To compare the difference of using HBsAg or HBeAg as screening index of HBV infection during operation.Methods:HBsAg and HBeAg were used as supervising and controlling indexes of HBV infection during operation,and the difference was compared in three aspects:the infective source,the spreading route and the susceptible person.Results:HBV infective threshold value of single layer gloves was 105 ID/ml.The ratio of patients with HBsAg positive,HBeAg negative and HBeAg positive,whose infectivity had reached HBV infective threshold value,were 19.23%,3.20% and 83.88%,respectively.The difference was significant (P<0.01).After healthcare workers were inoculated against HBV,the HBV infective threshold value was 108.5 ID/ml during surgical operation;0.64% of the infectious HBsAg positive carriers exceeded the infective threshold value.Conclusions:HBsAg and HBeAg are greatly different as supervising and controlling indexes of HBV infection in infective source,spread route and susceptible person.
2010, 35(6): 631-632,635.
Abstract:
Objective:To explore the effect of wet healing method in treatment of chronic wounds.Methods:Ninety-six patients with chronic wounds were randomly divided into two groups.In addition to the routine care including rotating mattress,clearing skin and nutritional support,the treatment group were applied wound covering with anabrosis powder and moist dressings,and the control group were given iodine eraser and the infrared light to roast the wounds.The effective rate of one course and the healing time after 3 courses were analyzed statistically.Results:The healing time was shorter and the efficacy was more obvious in the treatment group than in the control (P<0.01).Conclusions:The wet healing method has the advantages of affirmed effect and shorter treatment course in management of chronic wound.
Objective:To explore the effect of wet healing method in treatment of chronic wounds.Methods:Ninety-six patients with chronic wounds were randomly divided into two groups.In addition to the routine care including rotating mattress,clearing skin and nutritional support,the treatment group were applied wound covering with anabrosis powder and moist dressings,and the control group were given iodine eraser and the infrared light to roast the wounds.The effective rate of one course and the healing time after 3 courses were analyzed statistically.Results:The healing time was shorter and the efficacy was more obvious in the treatment group than in the control (P<0.01).Conclusions:The wet healing method has the advantages of affirmed effect and shorter treatment course in management of chronic wound.
2010, 35(6): 633-635.
Abstract:
Objective:To observe the effect of nursing intervention in one-time portable flexible infusion pump application.Methods:The observe group (30 cases) were treated with a daily injection;the length of the pump column was observed,measured and recorded every 3 hours;the remaining amount of drugs was calculated,and appropriate nursing care was given.The control group (30 cases) were treated with a one-time injection and given the routine nursing care.The accuracy of pump infusion and the adverse reactions after treatment were compared between the two groups.Results:The infusion time was more accurate and there was less phlebitis caused by medication in the observe group than in the control (P<0.01 and P<0.05).Conclusions:By taking positive and effective nursing intervention,the drugs can be safely,uniformly and accurately injected into the body,which may reduce the adverse reactions after treatment and improve patients' satisfaction degree.It is worth popularizing in clinical practice.
Objective:To observe the effect of nursing intervention in one-time portable flexible infusion pump application.Methods:The observe group (30 cases) were treated with a daily injection;the length of the pump column was observed,measured and recorded every 3 hours;the remaining amount of drugs was calculated,and appropriate nursing care was given.The control group (30 cases) were treated with a one-time injection and given the routine nursing care.The accuracy of pump infusion and the adverse reactions after treatment were compared between the two groups.Results:The infusion time was more accurate and there was less phlebitis caused by medication in the observe group than in the control (P<0.01 and P<0.05).Conclusions:By taking positive and effective nursing intervention,the drugs can be safely,uniformly and accurately injected into the body,which may reduce the adverse reactions after treatment and improve patients' satisfaction degree.It is worth popularizing in clinical practice.
2010, 35(6): 636-638.
Abstract:
Objective:To find a best site to catheterize for long-term hemodialysis.Methods:One hundred and eighty-five cases of hemodialysis were divided,according to the catheterization sites,into internal jugular vein group (95 cases),external jugular vein group (65 case) and femoral vein group (25 cases).The success rate of puncture,the incidence of complications during the puncture procedure and the catheterization were compared.Results:The success rates of puncture were 94.74%,93.84% and 92.00% in the internal jugular vein group,external jugular vein group and femoral vein group,respectively.The difference was not significant (P>0.05).The incidence of straying into the artery was significant higher in the femoral vein group than that in the other two groups (P<0.01),and the incidence of other complications had no significant difference among the three groups (P>0.05).The incidence of complication during catheterization had no significant difference between the internal jugular vein group and the external jugular vein group,while the femoral vein group presented a higher incidence of complication (P<0.01).Conclusions:The internal jugular vein puncture is a preferred means of catheterization for long-term hemodialysis,and the external jugular vein can be used as a second-class vessel for catheterization.The femoral vein can only be considered when the above veins are not accessible.
Objective:To find a best site to catheterize for long-term hemodialysis.Methods:One hundred and eighty-five cases of hemodialysis were divided,according to the catheterization sites,into internal jugular vein group (95 cases),external jugular vein group (65 case) and femoral vein group (25 cases).The success rate of puncture,the incidence of complications during the puncture procedure and the catheterization were compared.Results:The success rates of puncture were 94.74%,93.84% and 92.00% in the internal jugular vein group,external jugular vein group and femoral vein group,respectively.The difference was not significant (P>0.05).The incidence of straying into the artery was significant higher in the femoral vein group than that in the other two groups (P<0.01),and the incidence of other complications had no significant difference among the three groups (P>0.05).The incidence of complication during catheterization had no significant difference between the internal jugular vein group and the external jugular vein group,while the femoral vein group presented a higher incidence of complication (P<0.01).Conclusions:The internal jugular vein puncture is a preferred means of catheterization for long-term hemodialysis,and the external jugular vein can be used as a second-class vessel for catheterization.The femoral vein can only be considered when the above veins are not accessible.
2010, 35(6): 640-641.
Abstract:
Objective:To explore the influence of clinical nursing pathway (CNP) on children with viral encephalitis.Methods:One hundred and nineteen children with viral encephalitis were divided into the experimental group (59 cases) and the control group (60 cases) randomly.The routine nursing care was used in the control group,while the CNP was used in the experimental group.Results:The satisfactory degree in the CNP group was higher than that in the control group (P<0.001),and nursing defect of the CNP group was lower than that of the control group (P<0.05).Conclusions:The normalization and routinization of CNP can turn nursing from passiveness to initiative,so that the best nursing service can be achieved,and thus improve the nursing quality.
Objective:To explore the influence of clinical nursing pathway (CNP) on children with viral encephalitis.Methods:One hundred and nineteen children with viral encephalitis were divided into the experimental group (59 cases) and the control group (60 cases) randomly.The routine nursing care was used in the control group,while the CNP was used in the experimental group.Results:The satisfactory degree in the CNP group was higher than that in the control group (P<0.001),and nursing defect of the CNP group was lower than that of the control group (P<0.05).Conclusions:The normalization and routinization of CNP can turn nursing from passiveness to initiative,so that the best nursing service can be achieved,and thus improve the nursing quality.
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