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2010 Vol. 35, No. 8
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2010, 35(8): 757-761.
Abstract:
Objective: To observe the influence of 4-1BBL small interfering RNA (siRNA) on the growth and apoptosis of HL-60B cells.Methods: The siRNA for 4-1BBL gene was designed and synthesized in vitro,and then transfected into HL-60B cells by Lipofectamine;the expression of 4-1BBL mRNA and protein was detected by RT-PCR and flow cytometry,respectively;the proliferative rate of HL-60B cells was determined by methyl thiazoy tetralium (MTT),and the cell apoptosis was analyzed by flow cytometry.Results: After the siRNA for 4-1BBL gene was transfected into HL-60B cells,the expression of 4-1BBL gene and protein decreased,the growth of cells was repressed and the proliferation rate of HL-60B cells decreased.The inhibition rate was (22.1±2.3)% 48 h after transfection;the apoptosis rate increased from (9.8±2.5)% to (32.5±5.1)%.Conclusions: Chemically synthetic siRNA can decrease 4-1BBL gene expression,inhibit cellular proliferation and promote apoptosis in vitro.
Objective: To observe the influence of 4-1BBL small interfering RNA (siRNA) on the growth and apoptosis of HL-60B cells.Methods: The siRNA for 4-1BBL gene was designed and synthesized in vitro,and then transfected into HL-60B cells by Lipofectamine;the expression of 4-1BBL mRNA and protein was detected by RT-PCR and flow cytometry,respectively;the proliferative rate of HL-60B cells was determined by methyl thiazoy tetralium (MTT),and the cell apoptosis was analyzed by flow cytometry.Results: After the siRNA for 4-1BBL gene was transfected into HL-60B cells,the expression of 4-1BBL gene and protein decreased,the growth of cells was repressed and the proliferation rate of HL-60B cells decreased.The inhibition rate was (22.1±2.3)% 48 h after transfection;the apoptosis rate increased from (9.8±2.5)% to (32.5±5.1)%.Conclusions: Chemically synthetic siRNA can decrease 4-1BBL gene expression,inhibit cellular proliferation and promote apoptosis in vitro.
2010, 35(8): 762-763,767.
Abstract:
Objective: To elvaluate the relationship between the genetic polymorphism of cytochrome P450 1A1 (CYP1A1) and the genetic susceptibility in cervical cancer.Methods: The distribution frequencies of CYP1A1 Ile/Val polymorphism were detected in 92 cervical squamous cell carcinoma,31 cervical adenocarcinoma and 100 healthy controls by allele-specific PCR.Results: The frequencies of three genotypes Ile/Ile,Ile/Val,Val/Val were 33.0%,64.0% and 3.0% respectively in control group;20.7%,68.0% and 16.3% respectively in cervical squamous cell carcinoma;and 12.9%,67.7% and 19.4% respectively in cervical adenocarcinoma.There was the significant difference among the three groups (P<0.05).Conclusions: Polymorphism of CYP1A1 Ile/Val plays an important role in the oncogenesis of cervical cancer.
Objective: To elvaluate the relationship between the genetic polymorphism of cytochrome P450 1A1 (CYP1A1) and the genetic susceptibility in cervical cancer.Methods: The distribution frequencies of CYP1A1 Ile/Val polymorphism were detected in 92 cervical squamous cell carcinoma,31 cervical adenocarcinoma and 100 healthy controls by allele-specific PCR.Results: The frequencies of three genotypes Ile/Ile,Ile/Val,Val/Val were 33.0%,64.0% and 3.0% respectively in control group;20.7%,68.0% and 16.3% respectively in cervical squamous cell carcinoma;and 12.9%,67.7% and 19.4% respectively in cervical adenocarcinoma.There was the significant difference among the three groups (P<0.05).Conclusions: Polymorphism of CYP1A1 Ile/Val plays an important role in the oncogenesis of cervical cancer.
2010, 35(8): 764-767.
Abstract:
Objective: To investigate the regularity of callosal development in children and adolescent and its sex difference.Methods: The midsagittal images of 81 normal children and adolescent were selected in e-film workstation and then imported to the Photoshop.The area of the cerebrum and the corpus callosum were measured in Adobe Photoshop® 10.0.Pearson correlation analysis and test were adopted to analyze the development of corpus callosum and the sex difference.Results: In the total samples,significant linear correlation was observed between the corpus callosum/all the subregions and the age (P<0.05-P<0.01);when the analyses were performed separately for males and females,all subregions still linearly correlated with the age except areaⅠ (P>0.05).Although significant sexual difference was found in the total callosal area,as well as in the areaⅠ,Ⅳ and Ⅵ (P<0.05-P<0.01),the difference was not statistically significant (P>0.05) after the cerebral volume was corrected.Conclusions: In normal children and adolescent,the callosal area increases with the age,and all the subregions increase simultaneously.Sex difference of callosal area may be better explained by an underlying effect of cerebral size.
Objective: To investigate the regularity of callosal development in children and adolescent and its sex difference.Methods: The midsagittal images of 81 normal children and adolescent were selected in e-film workstation and then imported to the Photoshop.The area of the cerebrum and the corpus callosum were measured in Adobe Photoshop® 10.0.Pearson correlation analysis and test were adopted to analyze the development of corpus callosum and the sex difference.Results: In the total samples,significant linear correlation was observed between the corpus callosum/all the subregions and the age (P<0.05-P<0.01);when the analyses were performed separately for males and females,all subregions still linearly correlated with the age except areaⅠ (P>0.05).Although significant sexual difference was found in the total callosal area,as well as in the areaⅠ,Ⅳ and Ⅵ (P<0.05-P<0.01),the difference was not statistically significant (P>0.05) after the cerebral volume was corrected.Conclusions: In normal children and adolescent,the callosal area increases with the age,and all the subregions increase simultaneously.Sex difference of callosal area may be better explained by an underlying effect of cerebral size.
2010, 35(8): 768-770.
Abstract:
Objective: To study the significance of endoplasmic reticulum stress in hepatocytes apoptosis.Methods: The BALB/c mouse primary hepatocytes were isolated by two-step in situ liver perfusion,and then induced apoptosis with 4 μmol/L thapsigargin.siRNA against caspase-12 were synthesized chemically,then transfected into mouse primary hepatocytes by liposomes.Efficacy of caspase-12-siRNA inhibition was analyzed with RT-PCR and Western blot;viability of hepatocyte following apoptosis was evaluated with MTT.Results: The inhibition of caspase-12 mRNA and procaspase-12 expression were 86.22% and 50.04% respectively with 200 nmol/L siRNA (P<0.01).Contrasted with apoptotic hepatocytes,the cell activity analyzed with MTT increased by 51.65% (P<0.05).Conclusions: The mouse primary hepatocyte apoptosis can be reduced when endoplasmic reticulum stress-induced caspase-12 is inhibited.Endoplasmic reticulum stress plays an important role in hepatocyte apoptosis.
Objective: To study the significance of endoplasmic reticulum stress in hepatocytes apoptosis.Methods: The BALB/c mouse primary hepatocytes were isolated by two-step in situ liver perfusion,and then induced apoptosis with 4 μmol/L thapsigargin.siRNA against caspase-12 were synthesized chemically,then transfected into mouse primary hepatocytes by liposomes.Efficacy of caspase-12-siRNA inhibition was analyzed with RT-PCR and Western blot;viability of hepatocyte following apoptosis was evaluated with MTT.Results: The inhibition of caspase-12 mRNA and procaspase-12 expression were 86.22% and 50.04% respectively with 200 nmol/L siRNA (P<0.01).Contrasted with apoptotic hepatocytes,the cell activity analyzed with MTT increased by 51.65% (P<0.05).Conclusions: The mouse primary hepatocyte apoptosis can be reduced when endoplasmic reticulum stress-induced caspase-12 is inhibited.Endoplasmic reticulum stress plays an important role in hepatocyte apoptosis.
2010, 35(8): 771-775.
Abstract:
Objective: To investigate the expression of matrix metalloproteinase-1 (MMP-1) and vascular endothelial growth factor-C (VEGF-C) in human esophageal squamous cell carcinoma (ESCC),and their relationship with the clinicopathological parameters.Methods: The expressions of MMP-1 and VEGF-C were determined immunohistochemically in 90 cases of human esophageal squamous cell carcinoma,16 cases of severe dysplasia/carcinoma in situ and 28 cases of adjacent normal tissues.Results: The positive expression rates of MMP-1 in ESCC,severe dysplasia/carcinoma in situ and adjacent normal tissues were 81.11%,81.25% and 17.86%,respectively.The expression of MMP-1 was associated with the depth of invasion,lymph node metastasis and clinical staging in ESCC,but not with differentiation (P>0.05).The positive expression rates of VEGF-C in ESCC,severe dysplasia/carcinoma in situ and adjacent normal tissues were 83.33%,75.00% and 25.00%,respectively (P<0.01);the expression of VEGF-C was associated with the lymph node metastasis and clinical staging in ESCC (P<0.01 and P<0.05),but not with the differentiation or depth of invasion (P>0.05).And there was a positive correlation between MMP-1 and VEGF-C (P<0.01).Conclusions: MMP-1 and VEGF-C play a crucial role in invasion and lymphatic metastasis.They are important predictors of biological behavior in ESCC.
Objective: To investigate the expression of matrix metalloproteinase-1 (MMP-1) and vascular endothelial growth factor-C (VEGF-C) in human esophageal squamous cell carcinoma (ESCC),and their relationship with the clinicopathological parameters.Methods: The expressions of MMP-1 and VEGF-C were determined immunohistochemically in 90 cases of human esophageal squamous cell carcinoma,16 cases of severe dysplasia/carcinoma in situ and 28 cases of adjacent normal tissues.Results: The positive expression rates of MMP-1 in ESCC,severe dysplasia/carcinoma in situ and adjacent normal tissues were 81.11%,81.25% and 17.86%,respectively.The expression of MMP-1 was associated with the depth of invasion,lymph node metastasis and clinical staging in ESCC,but not with differentiation (P>0.05).The positive expression rates of VEGF-C in ESCC,severe dysplasia/carcinoma in situ and adjacent normal tissues were 83.33%,75.00% and 25.00%,respectively (P<0.01);the expression of VEGF-C was associated with the lymph node metastasis and clinical staging in ESCC (P<0.01 and P<0.05),but not with the differentiation or depth of invasion (P>0.05).And there was a positive correlation between MMP-1 and VEGF-C (P<0.01).Conclusions: MMP-1 and VEGF-C play a crucial role in invasion and lymphatic metastasis.They are important predictors of biological behavior in ESCC.
2010, 35(8): 776-778,781.
Abstract:
Objective: To investigate the effect of target controlled infusion (TCI) with midazolam for shivering in patients undergoing epidural anesthesia.Methods: Forty patients undergoing lower-abdominal operation with shivering were randomly divided into two equal groups (n=20,respectively):group A combined TCI with midazolam,while group B combined intravenous injection with droperidol-fentany.The changes of vital sign were monitored before and during operation.The sedation degree was evaluated by the Observer's Assessment of Alertness/Sedation (OAA/S) Scale.The therapeutic effect of shivering was estimated.Results: The numbers of mean arterial pressure (MAP),heart rate (HR) and pulse oxygen saturation (SpO2) were stable during the operation between two groups.They were within normal limits.Although the numbers of MAP and HR in group B after administration were lower than preoperative,the patients need not be treated.There were no significant differences in MAP and HR at different periods after the administration between two groups (P>0.05).While there was significant difference in SpO2 at minite 10 (P<0.01).The effective power of treating shiver of group A was better than group B (P<0.01).There was significant difference in sedation scale at different periods after the administration between two groups (P<0.05 to P<0.01).The OAA/S scale in group A maintained 2 to 3.The scales were 3 to 4 in group B at minite 5 and 10.The sedation degree weakened along with time prolonged.Conclusions: TCI with midazolam for shivering in patients undergoing epidural anesthesia is safe and convenient and effective.
Objective: To investigate the effect of target controlled infusion (TCI) with midazolam for shivering in patients undergoing epidural anesthesia.Methods: Forty patients undergoing lower-abdominal operation with shivering were randomly divided into two equal groups (n=20,respectively):group A combined TCI with midazolam,while group B combined intravenous injection with droperidol-fentany.The changes of vital sign were monitored before and during operation.The sedation degree was evaluated by the Observer's Assessment of Alertness/Sedation (OAA/S) Scale.The therapeutic effect of shivering was estimated.Results: The numbers of mean arterial pressure (MAP),heart rate (HR) and pulse oxygen saturation (SpO2) were stable during the operation between two groups.They were within normal limits.Although the numbers of MAP and HR in group B after administration were lower than preoperative,the patients need not be treated.There were no significant differences in MAP and HR at different periods after the administration between two groups (P>0.05).While there was significant difference in SpO2 at minite 10 (P<0.01).The effective power of treating shiver of group A was better than group B (P<0.01).There was significant difference in sedation scale at different periods after the administration between two groups (P<0.05 to P<0.01).The OAA/S scale in group A maintained 2 to 3.The scales were 3 to 4 in group B at minite 5 and 10.The sedation degree weakened along with time prolonged.Conclusions: TCI with midazolam for shivering in patients undergoing epidural anesthesia is safe and convenient and effective.
2010, 35(8): 779-781.
Abstract:
Objective: To evaluate the efficacy,side-effects and quality of life in the advanced gastric cancer patients treated by paclitaxel (PTX) plus tegafur (FT-207) and leucovorin (LV) with or without calf spleen extraction injection.Methods: Forty-three eligible patients were randomly assigned to the treatment group and control group.In the treatment group,20 patients were treated with PTX+FT-207+LV+calf spleen extraction injection.In the control group,the regimen was the same as in the treatment group except calf spleen extraction injection.Results: Overall response rates were 65.0% and 34.8% (P<0.05) in the treatment group and control group,respectively.The median survival time was 12 months versus 10 months (P>0.05) in the treatment group and control group,respectively.The 1-year survival rate for treatment group and control group was 60.0% versus 21.7% (P<0.05),respectively.The clinical efficacy and the frequence of leukocyte reduction were better in the treatment group than in control group,the quality of life were improved more significantly than those in control group (P<0.05).Conclusions: Calf spleen extraction injection plus PTX+FT-207+LV regimen can improve the efficacy of the treatment,reduce the chemotherapy related toxicities of digestive tract and bone marrow,improve the quality of life and the tolerance of chemotherapy of advanced gastric cancer patients.
Objective: To evaluate the efficacy,side-effects and quality of life in the advanced gastric cancer patients treated by paclitaxel (PTX) plus tegafur (FT-207) and leucovorin (LV) with or without calf spleen extraction injection.Methods: Forty-three eligible patients were randomly assigned to the treatment group and control group.In the treatment group,20 patients were treated with PTX+FT-207+LV+calf spleen extraction injection.In the control group,the regimen was the same as in the treatment group except calf spleen extraction injection.Results: Overall response rates were 65.0% and 34.8% (P<0.05) in the treatment group and control group,respectively.The median survival time was 12 months versus 10 months (P>0.05) in the treatment group and control group,respectively.The 1-year survival rate for treatment group and control group was 60.0% versus 21.7% (P<0.05),respectively.The clinical efficacy and the frequence of leukocyte reduction were better in the treatment group than in control group,the quality of life were improved more significantly than those in control group (P<0.05).Conclusions: Calf spleen extraction injection plus PTX+FT-207+LV regimen can improve the efficacy of the treatment,reduce the chemotherapy related toxicities of digestive tract and bone marrow,improve the quality of life and the tolerance of chemotherapy of advanced gastric cancer patients.
2010, 35(8): 782-783.
Abstract:
Objective: To explore the association of subclinical diabetic peripheral neuropathy with glycosylated hemoglobin and the course of the disease.Methods: The nerve conduction velocity and somatosensory evoked potential test were performed in 102 diabetic patients with no lower extremity peripheral neuropathy symptoms or signs to detect the subclinicct diabetdeteic peripheral neuropathy;the correlation between the subclinical diabetic peripheral neuropath,the course of the disease and the glycosylated hemoglobin were analyzed statistically.Results: Abnormal nerve conduction velocity and somatosensory evoked potential test results were found in 37 of the 102 cases,which was somewhat associated with glycated hemoglobin and the course of the disease (P<0.01 and P<0.05).Conclusions: Detection of the nerve conduction velocity and somatosensory evoked potential testing can help to early diagnosis of subclinical peripheral neuropathy,and long course of disease and high glycosylated hemoglobin might increase the incidence of subclinical peripheral neuropath.
Objective: To explore the association of subclinical diabetic peripheral neuropathy with glycosylated hemoglobin and the course of the disease.Methods: The nerve conduction velocity and somatosensory evoked potential test were performed in 102 diabetic patients with no lower extremity peripheral neuropathy symptoms or signs to detect the subclinicct diabetdeteic peripheral neuropathy;the correlation between the subclinical diabetic peripheral neuropath,the course of the disease and the glycosylated hemoglobin were analyzed statistically.Results: Abnormal nerve conduction velocity and somatosensory evoked potential test results were found in 37 of the 102 cases,which was somewhat associated with glycated hemoglobin and the course of the disease (P<0.01 and P<0.05).Conclusions: Detection of the nerve conduction velocity and somatosensory evoked potential testing can help to early diagnosis of subclinical peripheral neuropathy,and long course of disease and high glycosylated hemoglobin might increase the incidence of subclinical peripheral neuropath.
2010, 35(8): 784-785.
Abstract:
Objective: To explore the therapeutic effect of nasal endoscopic three minus septoplasty on deviation of nasal septum.Methods: Forty-six patients with deviation of nasal septum were treated by nasal endoscopic three minus septoplasty.Results: During the follow-up of 3-6 months,42 cases healed and 4 cases improved.No perforation of nasal septum or synechia nasal occurred.Conclusions: Nasal endoscopic three minus septoplasty is safe and effective in treatment of deviation of nasal septum.It is worth popularizing.
Objective: To explore the therapeutic effect of nasal endoscopic three minus septoplasty on deviation of nasal septum.Methods: Forty-six patients with deviation of nasal septum were treated by nasal endoscopic three minus septoplasty.Results: During the follow-up of 3-6 months,42 cases healed and 4 cases improved.No perforation of nasal septum or synechia nasal occurred.Conclusions: Nasal endoscopic three minus septoplasty is safe and effective in treatment of deviation of nasal septum.It is worth popularizing.
2010, 35(8): 786-787.
Abstract:
Objective: To evaluate the combination therapy of minimally invasive technique plus percutaneous locking plate fixation for treatment of proximal humeral fractures in the elderly.Methods: Eighteen elderly patients with proximal humeral fractures were treated by minimally invasive technique and percutaneous locking plate fixation.Results: The patients were followed up for 4-12 months.All the fractures healed.According to Neer standard score,the result was excellent in 13 cases,good in 3 cases and fair in 2 cases.During the follow-up,1 patient died of diabetes mellitus complicated with pulmonary infection.Conclusions: Percutaneous micro-invasive technology combined with locking plate fixation is safe and effective for treatment of proximal humeral fractures in the elderly,and the results are satisfactory.
Objective: To evaluate the combination therapy of minimally invasive technique plus percutaneous locking plate fixation for treatment of proximal humeral fractures in the elderly.Methods: Eighteen elderly patients with proximal humeral fractures were treated by minimally invasive technique and percutaneous locking plate fixation.Results: The patients were followed up for 4-12 months.All the fractures healed.According to Neer standard score,the result was excellent in 13 cases,good in 3 cases and fair in 2 cases.During the follow-up,1 patient died of diabetes mellitus complicated with pulmonary infection.Conclusions: Percutaneous micro-invasive technology combined with locking plate fixation is safe and effective for treatment of proximal humeral fractures in the elderly,and the results are satisfactory.
2010, 35(8): 788-790,793.
Abstract:
Objective: To determine the mutation status of epidermal growth factor receptor (EGFR) in chinese non-small-cell lung cancer (NSCLC) patients,and after detected a rare mutant type of EGFR,P848L,to further study its functional feature.Methods: Fiftyfive NSCLC samples were enrolled in the study.The total DNA was extracted,then the 1-27 exons were sequenced for the detection of mutation.The P848L mutant EGFR construct and then the T790M mutation was generated by introducing a point mutation into the wildtype EGFR vector using a site-directed mutagenesis kit.Results: There were 8 cases of mutations being detected,all the mutationharboring patients were non-smoker with pathologic type of adenocarcinoma.In vitro study demonstrated that the P848L mutant had a similar response to gefitinib treatment,and P848L and T790M double mutant did respond to gefitinib.Conclusions: EGFR mutations occurred in only non-smoker patients with lung adenocarcinoma in this series.P848L mutant EGFR has a similar response as the widetype to gefitinib treatment,while double mutated with T790M diminished the response completely.
Objective: To determine the mutation status of epidermal growth factor receptor (EGFR) in chinese non-small-cell lung cancer (NSCLC) patients,and after detected a rare mutant type of EGFR,P848L,to further study its functional feature.Methods: Fiftyfive NSCLC samples were enrolled in the study.The total DNA was extracted,then the 1-27 exons were sequenced for the detection of mutation.The P848L mutant EGFR construct and then the T790M mutation was generated by introducing a point mutation into the wildtype EGFR vector using a site-directed mutagenesis kit.Results: There were 8 cases of mutations being detected,all the mutationharboring patients were non-smoker with pathologic type of adenocarcinoma.In vitro study demonstrated that the P848L mutant had a similar response to gefitinib treatment,and P848L and T790M double mutant did respond to gefitinib.Conclusions: EGFR mutations occurred in only non-smoker patients with lung adenocarcinoma in this series.P848L mutant EGFR has a similar response as the widetype to gefitinib treatment,while double mutated with T790M diminished the response completely.
2010, 35(8): 791-793.
Abstract:
Objective: To investigate the effect of the island skin flap with sural nerve nutrient vessels on repairing skin defects in ankle and foot.Methods: Twenty patients with skin defects in ankle and foot were treated by improved island skin flaps with sural nerve nutrient vessels.The flap area ranged from 4 cm×5 cm to 11 cm×10 cm.The donor sites were sutured directly or covered with free flaps.Results: Eighteen flaps survived,and the donor and recipient sites healed well;two cases presented distal necrosis.Twelve cases were followed up for 6 to 12 months;the contour of the flap was satisfactory and the color was similar to that of normal skin;no necrosis occurred.Conclusions: The island skin flap with sural nerve nutrient vessels is an ideal flap for repairing skin defects in ankle and foot.
Objective: To investigate the effect of the island skin flap with sural nerve nutrient vessels on repairing skin defects in ankle and foot.Methods: Twenty patients with skin defects in ankle and foot were treated by improved island skin flaps with sural nerve nutrient vessels.The flap area ranged from 4 cm×5 cm to 11 cm×10 cm.The donor sites were sutured directly or covered with free flaps.Results: Eighteen flaps survived,and the donor and recipient sites healed well;two cases presented distal necrosis.Twelve cases were followed up for 6 to 12 months;the contour of the flap was satisfactory and the color was similar to that of normal skin;no necrosis occurred.Conclusions: The island skin flap with sural nerve nutrient vessels is an ideal flap for repairing skin defects in ankle and foot.
2010, 35(8): 794-796.
Abstract:
Objective: To investigate the risk factors leading to death for patients with dermatomyositis (DM).Methods: The clinical data of 156 patients with DM including 44 patients who had died were analyzed retrospectively.Results: The mortality rate was 22.2%,with the male accounting for 39.6% and the female 13.5% (P<0.01).The peak age of onset ranged from 56 to 65.The mortality rate in cases with acute onset and subacute onset was 33.3% and 29.5%,respectively,which was higher than that with chronic onset (12.3%) (P<0.05).Among the 44 cases who had died,29 cases had a disease course of less than 1 year and 37 cases less than 3 years,the mortality of which was higher than that over 3 years;18 of the cases were complicated with malignant tumors and 18 interstitial lung disease (ILD) (including 5 secondary infections);6 cases died of pulmonary infection and 2 unknown causes.The serum level of lactate dehydrogenase (LDH),γ-glutamyltransferase (GGT),erythrocyte sedimentation rate (ESR) and gamma globulin were higher in the patients with poor outcome than that in those with fine outcome (P<0.05-P<0.01).There were significant difference in arthralgia,fever and electrocardiographic abnormality between patients with poor outcome and fine outcome (P<0.01).The differences in serum levels of CK,positive anti-ANA,positive anti-dsDNA,positive anti-Jo-1 antibody and the skin rashes were not significant between the two patient groups (P>0.05).Conclusions: These data indicate that the mortality of dermatomyositis occur mostly in the first 3 years of onset,especially the first year.The risk factors leading to death for DM patients are malignancy,ILD,pulmonary infection,acute and subacute onsets,old age,maleness,arthralgia,fever and electrocardiographic abnormality.The serum levels of GGT,LDH,ESR and gamma globulin have prognostic value for the DM patients.
Objective: To investigate the risk factors leading to death for patients with dermatomyositis (DM).Methods: The clinical data of 156 patients with DM including 44 patients who had died were analyzed retrospectively.Results: The mortality rate was 22.2%,with the male accounting for 39.6% and the female 13.5% (P<0.01).The peak age of onset ranged from 56 to 65.The mortality rate in cases with acute onset and subacute onset was 33.3% and 29.5%,respectively,which was higher than that with chronic onset (12.3%) (P<0.05).Among the 44 cases who had died,29 cases had a disease course of less than 1 year and 37 cases less than 3 years,the mortality of which was higher than that over 3 years;18 of the cases were complicated with malignant tumors and 18 interstitial lung disease (ILD) (including 5 secondary infections);6 cases died of pulmonary infection and 2 unknown causes.The serum level of lactate dehydrogenase (LDH),γ-glutamyltransferase (GGT),erythrocyte sedimentation rate (ESR) and gamma globulin were higher in the patients with poor outcome than that in those with fine outcome (P<0.05-P<0.01).There were significant difference in arthralgia,fever and electrocardiographic abnormality between patients with poor outcome and fine outcome (P<0.01).The differences in serum levels of CK,positive anti-ANA,positive anti-dsDNA,positive anti-Jo-1 antibody and the skin rashes were not significant between the two patient groups (P>0.05).Conclusions: These data indicate that the mortality of dermatomyositis occur mostly in the first 3 years of onset,especially the first year.The risk factors leading to death for DM patients are malignancy,ILD,pulmonary infection,acute and subacute onsets,old age,maleness,arthralgia,fever and electrocardiographic abnormality.The serum levels of GGT,LDH,ESR and gamma globulin have prognostic value for the DM patients.
2010, 35(8): 797-798,800.
Abstract:
Objective: To investigate the causes,management and preventive measures for anastomotic leakage after radical resection for rectal cancer.Methods: From Jan.2002 to Aug.2009,11 cases of anastomic leakage after 112 cases of anus-preserving operation were retrospectively analyzed.Results: The incidence of anastomotic leakage was 9.8%.Ten of the cases received non-surgical treatment and 1 case recovered after re-operation.Conclusions: Adequate preoperative preparation,proper attention to the blood supply and tension of the anastomosis,rational use of the stapler,reasonable selection of the drainage tube are key to the prevention of fistula.By effective and timely drainage,most of the anastomotic leakage can be cured.
Objective: To investigate the causes,management and preventive measures for anastomotic leakage after radical resection for rectal cancer.Methods: From Jan.2002 to Aug.2009,11 cases of anastomic leakage after 112 cases of anus-preserving operation were retrospectively analyzed.Results: The incidence of anastomotic leakage was 9.8%.Ten of the cases received non-surgical treatment and 1 case recovered after re-operation.Conclusions: Adequate preoperative preparation,proper attention to the blood supply and tension of the anastomosis,rational use of the stapler,reasonable selection of the drainage tube are key to the prevention of fistula.By effective and timely drainage,most of the anastomotic leakage can be cured.
2010, 35(8): 799-800.
Abstract:
Objective: To investigate the effect of regional excision of parotid gland in treatment of benign tumor.Methods: A retrospective analysis of 67 cases of parotid gland superficial mass was carried out.Forty-four cases were proved to be pleomorphic adenoma of parotid and 23 cases papillary cystadenoma lympho-matosum of parotid.All the cases underwent regional excision of parotid gland.Results: All the cases were cured at one stage.Four cases presented temporary facial paralysis,which were treated by vitamin B1 and vitamin B2 injection and recovered within 1 to 3 months.The regional excision had the advantages of shorter operation time and less complication.No relapse was observed during the 1-7 year follow-up.Conclusions: Regional excision of parotid gland should be advocated for treatment of benign tumor.
Objective: To investigate the effect of regional excision of parotid gland in treatment of benign tumor.Methods: A retrospective analysis of 67 cases of parotid gland superficial mass was carried out.Forty-four cases were proved to be pleomorphic adenoma of parotid and 23 cases papillary cystadenoma lympho-matosum of parotid.All the cases underwent regional excision of parotid gland.Results: All the cases were cured at one stage.Four cases presented temporary facial paralysis,which were treated by vitamin B1 and vitamin B2 injection and recovered within 1 to 3 months.The regional excision had the advantages of shorter operation time and less complication.No relapse was observed during the 1-7 year follow-up.Conclusions: Regional excision of parotid gland should be advocated for treatment of benign tumor.
2010, 35(8): 801-803.
Abstract:
Objective: To explore the causes of recurrent laryngeal nerve (RLN) trauma due to operations for thyroid lesion and the preventive measures.Methods: The surgical procedures for thyroid lesion,the correlation between whether the recurrent laryngeal nerve was isolated and protected in the operation and the recurrent laryngeal nerve trauma were analyzed retrospectively.Results: Three hundred and nineteen patients received complete resection.Three cases presented temporary trauma of recurrent laryngeal nerve,and no postoperative hemorrhoea or thyroid crisis occurred.Conclusions: The anatomy of the recurrent laryngeal nerve is complicated.The key to reducing RLN trauma is to know well the anatomy and the variation of recurrent laryngealn nerve,and to reveal the recurrent laryngeal nerve accordingly in the surgical operation for thyroid lesions.
Objective: To explore the causes of recurrent laryngeal nerve (RLN) trauma due to operations for thyroid lesion and the preventive measures.Methods: The surgical procedures for thyroid lesion,the correlation between whether the recurrent laryngeal nerve was isolated and protected in the operation and the recurrent laryngeal nerve trauma were analyzed retrospectively.Results: Three hundred and nineteen patients received complete resection.Three cases presented temporary trauma of recurrent laryngeal nerve,and no postoperative hemorrhoea or thyroid crisis occurred.Conclusions: The anatomy of the recurrent laryngeal nerve is complicated.The key to reducing RLN trauma is to know well the anatomy and the variation of recurrent laryngealn nerve,and to reveal the recurrent laryngeal nerve accordingly in the surgical operation for thyroid lesions.
2010, 35(8): 805-808.
Abstract:
Objective: To evaluate the efficacy of tirofiban by intracoronary administration on TIMI flow in acute coronary syndrome (ACS) with no-reflow phenomenon after percutaneous coronary intervention (PCI).Methods: Forty-eight ACS patients with no-reflow phenomenon after PCI were randomized to tirofiban group (intracoronary verapamil and tirofiban,10 μg/kg,n=25) and verapamil group (intracoronary verapamil,200 μg,n=23).The TIMI flow at 30 min post-medication,corrected TIMI frame count (CTFC),left ventricular ejection fraction after PCI for 7 days,hemorrhage complication and incidence of major adverse cardiovascular events (MACE) within 30 days after PCI were observed in both groups.Results: The TIMI 3 ratio in tirofiban group (64.0%) was markedly higher than that in verapamil group (34.8%) (P<0.05);CTFC showed that the coronary blood flow in tirofiban group was faster than that in verapamil group (P<0.01);the left ventricular ejection fraction after PCI for 7 days was higher in tirofiban group than in verapamil group (P<0.01);the difference between the hemorrhage complication and the incidence of MACE within 30 days after PCI was not significant (P>0.05).Conclusions: Intracoronary administration of tirofiban is effective and safe for ACS patients with noreflow phenomenon after PCI.
Objective: To evaluate the efficacy of tirofiban by intracoronary administration on TIMI flow in acute coronary syndrome (ACS) with no-reflow phenomenon after percutaneous coronary intervention (PCI).Methods: Forty-eight ACS patients with no-reflow phenomenon after PCI were randomized to tirofiban group (intracoronary verapamil and tirofiban,10 μg/kg,n=25) and verapamil group (intracoronary verapamil,200 μg,n=23).The TIMI flow at 30 min post-medication,corrected TIMI frame count (CTFC),left ventricular ejection fraction after PCI for 7 days,hemorrhage complication and incidence of major adverse cardiovascular events (MACE) within 30 days after PCI were observed in both groups.Results: The TIMI 3 ratio in tirofiban group (64.0%) was markedly higher than that in verapamil group (34.8%) (P<0.05);CTFC showed that the coronary blood flow in tirofiban group was faster than that in verapamil group (P<0.01);the left ventricular ejection fraction after PCI for 7 days was higher in tirofiban group than in verapamil group (P<0.01);the difference between the hemorrhage complication and the incidence of MACE within 30 days after PCI was not significant (P>0.05).Conclusions: Intracoronary administration of tirofiban is effective and safe for ACS patients with noreflow phenomenon after PCI.
2010, 35(8): 817-819.
Abstract:
Objective: To investigate the resolution of sampling probe towing positive in automatic enzyme immunoassay instrument.Methods: Anti-human immunodeficiency virus (HIV),anti-treponema pallidum (TP),anti-hepatitis C virus (HCV) or hepatitis B surface antigen (HBsAg) strong positive specimens were collected from routine screening test.The towing positive simulation test was performed in RMP 200 automatic enzyme immunoassay under use of demineralized water or 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions conditions.By comparison of the two groups results,it was observed that 0.1% Tween-20 weak alkaline solution in role of avoid sampling probe towing positive and it effect on ELISA.Results: It was found that every of anti-HIV,anti-TP,anti-HCV,and HBsAg sepecimen had experienced towing positive under use of demineralized water for sampling probe irrigation solutions.Among them,anti-HIV towing positive was most obvious and had 6 towing positive holes;while HBsAg appeared only a towing weak positive hole.In contrast to demineralized water,all towing positive phenomena of anti-HIV,anti-TP,anti-HCV and HBsAg strong positive specimens were avoided under use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions.Moreover,the results of quality control serum,positive and negative control under two different irrigation solutions had no significant difference for the same test item (P>0.05).Conclusions: The use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions could avoid appearance of sampling probe towing positive in automatic enzyme immunoassay instrument and it had no significant effect on ELISA.
Objective: To investigate the resolution of sampling probe towing positive in automatic enzyme immunoassay instrument.Methods: Anti-human immunodeficiency virus (HIV),anti-treponema pallidum (TP),anti-hepatitis C virus (HCV) or hepatitis B surface antigen (HBsAg) strong positive specimens were collected from routine screening test.The towing positive simulation test was performed in RMP 200 automatic enzyme immunoassay under use of demineralized water or 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions conditions.By comparison of the two groups results,it was observed that 0.1% Tween-20 weak alkaline solution in role of avoid sampling probe towing positive and it effect on ELISA.Results: It was found that every of anti-HIV,anti-TP,anti-HCV,and HBsAg sepecimen had experienced towing positive under use of demineralized water for sampling probe irrigation solutions.Among them,anti-HIV towing positive was most obvious and had 6 towing positive holes;while HBsAg appeared only a towing weak positive hole.In contrast to demineralized water,all towing positive phenomena of anti-HIV,anti-TP,anti-HCV and HBsAg strong positive specimens were avoided under use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions.Moreover,the results of quality control serum,positive and negative control under two different irrigation solutions had no significant difference for the same test item (P>0.05).Conclusions: The use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions could avoid appearance of sampling probe towing positive in automatic enzyme immunoassay instrument and it had no significant effect on ELISA.
2010, 35(8): 820-822.
Abstract:
Objective: To monitor the composition and drug resistance of the pathogenic bacteria isolated from the urine of patients with urinary tract infections.Methods: One thousand one hundred and twenty-six drug-resistant bacteria isolated from urine were identified and analyzed,and the special drug-resistant strains were detected by K-B method.The sensitivity rate,intermediate rate and drug resistance rate were determined in accordance with CLSI 2006 standard.Results: Of the 1 126 strains isolated from patients with urinary tract infection,E.coli-based strains accounted for 45.5% (512/1 126),coagulase-negative Staphylococci 18.5% (208/1 126),Enterococcus 9.9% (112/1 126),Candida albicans 5.4% (61/1 126),Mycetozoan 4.9% (55/1 126),Klebsiella pneumoniae 4.6% (52/1 126),and the others were Enterobacter cloacae and Pseudomonas aeruginosa (Corynebacterium 4,such as Acinetobacter baumannii).The detection rate of ESBLs was 48.0% in E.coli,38.6% in Klebsiella pneumoniae,9.5% in Pseudomonas aeruginosa,78.5% in methicillin-resistant staphylococcus aureus,1.8% of vancomycin resistant enterococcal and 80.4% of high level aminoglikozyde resistance.Only 1.4% of Ampc was detected in E.coli strains.Carbapenems was still the most effective antibiotics for gram-negative bacteria (except maltophilia S.m and Flavobacterium).Cefoperazone/sulbactam,piperacillin/tazobactam and cefepime also presented good antibacterial activity.The resistance rate of Gram-positive bacteria was relatively high,only maintaining good sensitivity to vancomycin.Conclusions: Enterobacteriaceae and coagulase-negative staphylococci are the main pathogens for urinary tract infections.The resistant rate of a variety of pathogens tends to rise.Carbon penicillins and vancomycin are the best and strongest antibiotics for gram-negative bacilli and gram-positive cocci respectively,which is worth clinical attention.
Objective: To monitor the composition and drug resistance of the pathogenic bacteria isolated from the urine of patients with urinary tract infections.Methods: One thousand one hundred and twenty-six drug-resistant bacteria isolated from urine were identified and analyzed,and the special drug-resistant strains were detected by K-B method.The sensitivity rate,intermediate rate and drug resistance rate were determined in accordance with CLSI 2006 standard.Results: Of the 1 126 strains isolated from patients with urinary tract infection,E.coli-based strains accounted for 45.5% (512/1 126),coagulase-negative Staphylococci 18.5% (208/1 126),Enterococcus 9.9% (112/1 126),Candida albicans 5.4% (61/1 126),Mycetozoan 4.9% (55/1 126),Klebsiella pneumoniae 4.6% (52/1 126),and the others were Enterobacter cloacae and Pseudomonas aeruginosa (Corynebacterium 4,such as Acinetobacter baumannii).The detection rate of ESBLs was 48.0% in E.coli,38.6% in Klebsiella pneumoniae,9.5% in Pseudomonas aeruginosa,78.5% in methicillin-resistant staphylococcus aureus,1.8% of vancomycin resistant enterococcal and 80.4% of high level aminoglikozyde resistance.Only 1.4% of Ampc was detected in E.coli strains.Carbapenems was still the most effective antibiotics for gram-negative bacteria (except maltophilia S.m and Flavobacterium).Cefoperazone/sulbactam,piperacillin/tazobactam and cefepime also presented good antibacterial activity.The resistance rate of Gram-positive bacteria was relatively high,only maintaining good sensitivity to vancomycin.Conclusions: Enterobacteriaceae and coagulase-negative staphylococci are the main pathogens for urinary tract infections.The resistant rate of a variety of pathogens tends to rise.Carbon penicillins and vancomycin are the best and strongest antibiotics for gram-negative bacilli and gram-positive cocci respectively,which is worth clinical attention.
2010, 35(8): 823-825.
Abstract:
Objective: To monitor the distribution and drug resistance of clinically isolated common gram-negative bacilli so as to provide basis for appropriate use of antibiotics in clinic.Methods: The bacillus samples collected in the period of 2007 to 2009 were isolated and cultured according to the National Clinical Laboratory Procedures.The identification and drug susceptibility tests were carried out by using Micro Scan-40S semi-automatic microbiological assay and drug susceptibility system,and part of the strains was detected by K-B method.The results were evaluated in accordance with the standard of Clinical Laboratory Information Systems.Results: Eight hundred and fifty-five gram-negative bacilli were isolated.Escherichia coli,Pseudomonas aeruginosa and Acinetobacter baumann occupied the top three places.The detection rates of Escherichia coli and Klebsieua pneumoniae producing extended spectrum β lactamases were 58.6% and 29.7%,respectively;non-fermenting bacteria mainly consisted of Pseudomona aeruginosa,Acinetobacter Baumannii and Strenotrophomonas maltophilia;the sensitive rate of Escherichia coli,Klebsieua pneumoniae and Entrobacter to imipenem were all above 90.0%;the sensitive rate of Pseudomona aeruginosa to imipenem and amikacin was 62.4%,and to cefepime 43.5%;the sensitive rates of Acinetobacter Baumannii to imipenem,amikacin and luomefloxacia were 92.7%,63.4% and 60.2%,respectively;and the sensitive rate of Strenotrophomonas maltophilia to sulfamethoxazole was 100.0%.Conclusions: Clinicians should keep contact with the microbiology laboratory,be alert of the appearance of multi-resistant bacteria and use antibiotics individually and reasonably according to the drug sensitivity results so that the treatment effect could be improved and the emergence of resistant strains reduced.
Objective: To monitor the distribution and drug resistance of clinically isolated common gram-negative bacilli so as to provide basis for appropriate use of antibiotics in clinic.Methods: The bacillus samples collected in the period of 2007 to 2009 were isolated and cultured according to the National Clinical Laboratory Procedures.The identification and drug susceptibility tests were carried out by using Micro Scan-40S semi-automatic microbiological assay and drug susceptibility system,and part of the strains was detected by K-B method.The results were evaluated in accordance with the standard of Clinical Laboratory Information Systems.Results: Eight hundred and fifty-five gram-negative bacilli were isolated.Escherichia coli,Pseudomonas aeruginosa and Acinetobacter baumann occupied the top three places.The detection rates of Escherichia coli and Klebsieua pneumoniae producing extended spectrum β lactamases were 58.6% and 29.7%,respectively;non-fermenting bacteria mainly consisted of Pseudomona aeruginosa,Acinetobacter Baumannii and Strenotrophomonas maltophilia;the sensitive rate of Escherichia coli,Klebsieua pneumoniae and Entrobacter to imipenem were all above 90.0%;the sensitive rate of Pseudomona aeruginosa to imipenem and amikacin was 62.4%,and to cefepime 43.5%;the sensitive rates of Acinetobacter Baumannii to imipenem,amikacin and luomefloxacia were 92.7%,63.4% and 60.2%,respectively;and the sensitive rate of Strenotrophomonas maltophilia to sulfamethoxazole was 100.0%.Conclusions: Clinicians should keep contact with the microbiology laboratory,be alert of the appearance of multi-resistant bacteria and use antibiotics individually and reasonably according to the drug sensitivity results so that the treatment effect could be improved and the emergence of resistant strains reduced.
2010, 35(8): 826-827.
Abstract:
Objective: To know about the situation of hepatitis B virus infection and the liver function among college freshman,in order to provide the scientific basis for prevention and education work.Methods: Firstly used enzyme linked immunosorbent assay to check HBsAg carriers of freshmen 2006,2007,2008;then used kinetic method to determine the serum alanine aminotransferase (ALT).Results: The positive rates of HBsAg were 3.19%,3.38%,and 2.07% among freshmen 2006,2007,and 2008,respectively;and 2.87% among the total students.The positive rate was significantly lower than the level of national population seroepidemiological survey of hepatitis B (8.57%) in 2006.The difference of positive rates was statistically significant among freshmen 2006,2007,and 2008 (P < 0.05).The difference of positive rates was statistically significant between male students and female students (P<0.01).And the difference of positive rates was also statistically significant between male students and female students among freshmen 2006,2007,and 2008,respectively (P<0.01).The difference of abnormity rate about ALT was not statistically significant among freshmen 2006,2007,and 2008 (P>0.05).Conclusions: The positive rate of HBsAg is low among freshmen.The positive rate of male students is significantly higher than that of female students.The abnormity rate of ALT is low.The college must strengthen health education of the hepatitis B to the students,increase physical examination items and have them vaccinated against hepatitis B virus.
Objective: To know about the situation of hepatitis B virus infection and the liver function among college freshman,in order to provide the scientific basis for prevention and education work.Methods: Firstly used enzyme linked immunosorbent assay to check HBsAg carriers of freshmen 2006,2007,2008;then used kinetic method to determine the serum alanine aminotransferase (ALT).Results: The positive rates of HBsAg were 3.19%,3.38%,and 2.07% among freshmen 2006,2007,and 2008,respectively;and 2.87% among the total students.The positive rate was significantly lower than the level of national population seroepidemiological survey of hepatitis B (8.57%) in 2006.The difference of positive rates was statistically significant among freshmen 2006,2007,and 2008 (P < 0.05).The difference of positive rates was statistically significant between male students and female students (P<0.01).And the difference of positive rates was also statistically significant between male students and female students among freshmen 2006,2007,and 2008,respectively (P<0.01).The difference of abnormity rate about ALT was not statistically significant among freshmen 2006,2007,and 2008 (P>0.05).Conclusions: The positive rate of HBsAg is low among freshmen.The positive rate of male students is significantly higher than that of female students.The abnormity rate of ALT is low.The college must strengthen health education of the hepatitis B to the students,increase physical examination items and have them vaccinated against hepatitis B virus.
2010, 35(8): 828-830.
Abstract:
Objective: To evaluate the detection of reticulocytes by flow cytometry comprehensively.Methods: The percent of peripheral blood reticulocytes was detected by flow cytometry.The precision reproducibility and stability were observed.The results were compared with manual method and evaluated linearly.Results: Precision CV of the indexes was less than 0.36%,which had a good stability.And the percentage CV at 4℃ in 24 hours was less than 0.43%.With the prolongation of storage time of samples,the results increased slightly,but no effect on clinical diagnosis.It had a positive correlation with manual methods (P<0.01).Conclusions: Analyzing reticuloctyes by flow cytometry is a simple,fast,accurate,stable method and has a good reproducibility.It can meet clinical requirement and replace manual methods.
Objective: To evaluate the detection of reticulocytes by flow cytometry comprehensively.Methods: The percent of peripheral blood reticulocytes was detected by flow cytometry.The precision reproducibility and stability were observed.The results were compared with manual method and evaluated linearly.Results: Precision CV of the indexes was less than 0.36%,which had a good stability.And the percentage CV at 4℃ in 24 hours was less than 0.43%.With the prolongation of storage time of samples,the results increased slightly,but no effect on clinical diagnosis.It had a positive correlation with manual methods (P<0.01).Conclusions: Analyzing reticuloctyes by flow cytometry is a simple,fast,accurate,stable method and has a good reproducibility.It can meet clinical requirement and replace manual methods.
2010, 35(8): 832-833,836.
Abstract:
Objective: To evaluate CT in diagnosis of renal milk of calcium (RMC).Methods: The X-ray films and CT findings of 12 cases of RMC were reviewed by two experienced radiologists by double blind method.All the cases underwent anterior-posterior and lateral abdomen plain film examination and non-enhanced CT scans.Among them,3 cases were performed enhanced CT scan.Results: The CT findings clearly displayed the whole view of milky calcium cyst and its'half-moon'sign.The half-moon's high density shadow formed by RMC was always located in the lowest part of the lesion.The cyst type and hydronephrosis type were observed in 5 cases and 7 cases respectively;the lesions of all but 2 cases were unilateral;RMC accompanied by renal stone was found in 2 cases.Four cases of RMC were misdiagnosed as renal stone and 2 cases were miss-diagnosed by X-ray.Conclusions: CT plays an important role in diagnosis of stone and its sensitivity is superior to X-ray films.
Objective: To evaluate CT in diagnosis of renal milk of calcium (RMC).Methods: The X-ray films and CT findings of 12 cases of RMC were reviewed by two experienced radiologists by double blind method.All the cases underwent anterior-posterior and lateral abdomen plain film examination and non-enhanced CT scans.Among them,3 cases were performed enhanced CT scan.Results: The CT findings clearly displayed the whole view of milky calcium cyst and its'half-moon'sign.The half-moon's high density shadow formed by RMC was always located in the lowest part of the lesion.The cyst type and hydronephrosis type were observed in 5 cases and 7 cases respectively;the lesions of all but 2 cases were unilateral;RMC accompanied by renal stone was found in 2 cases.Four cases of RMC were misdiagnosed as renal stone and 2 cases were miss-diagnosed by X-ray.Conclusions: CT plays an important role in diagnosis of stone and its sensitivity is superior to X-ray films.
2010, 35(8): 834-836.
Abstract:
Objective: To discuss the cardiac toxicity of anthracycline by observing the electrocardiogram which recording the routs of administration by arteries providing nutrients to the tumor.Methods: Eighty-eight cases which were pathologically diagnosed as midadvanced malignant tumor were randomly divided into group A and group B (44 cases for each group).The chemotherapy regimen for both groups consisted of adriamycin + cisplatin + fluorouracil.In group A,regional perfusion through main coronary arteries of tumors was used alone for 56 times;in Group B,combined application of perfusion,regionally and super-selectively,with integration of multi-targeted intubation,reasonable and flexible embolization,low-dose of medication and perfusion according to proportion of blood supply,was conducted for 88 times.Infusion chemotherapy and embolization were conducted 144 times altogether.The conventional 12-lead ecg was recorded 10 minutes,24 hours and 48 hours after operation and drug injection.Results: Eighty-eight patients' electrocardiograms were normal during the process of pre-intubation and while-intubation.After conducting anticancer drug perfusion for 144 times,64 cases presented changes of electrocardiograms (44.5%),and the incidence rate of arrhythmia was 58.1% in group A.The difference between the two groups was statistically significant (P<0.01).The incidence of arrhythmia in patients aged above or equal to 60 was higher than that aged between 50 and 59 (P<0.05).Conclusions: Regarding to the two methods of interventional chemotherapy,the incidence of arrhythmia is higher in Group A.That implies that the cardiac toxicity initiated by the method used in Group A is not different from that initiated by the method of intravenous chemotherapy,and such effect occurs frequently among the elderly,which should be highly alerted to in the process of interventional chemotherapy.
Objective: To discuss the cardiac toxicity of anthracycline by observing the electrocardiogram which recording the routs of administration by arteries providing nutrients to the tumor.Methods: Eighty-eight cases which were pathologically diagnosed as midadvanced malignant tumor were randomly divided into group A and group B (44 cases for each group).The chemotherapy regimen for both groups consisted of adriamycin + cisplatin + fluorouracil.In group A,regional perfusion through main coronary arteries of tumors was used alone for 56 times;in Group B,combined application of perfusion,regionally and super-selectively,with integration of multi-targeted intubation,reasonable and flexible embolization,low-dose of medication and perfusion according to proportion of blood supply,was conducted for 88 times.Infusion chemotherapy and embolization were conducted 144 times altogether.The conventional 12-lead ecg was recorded 10 minutes,24 hours and 48 hours after operation and drug injection.Results: Eighty-eight patients' electrocardiograms were normal during the process of pre-intubation and while-intubation.After conducting anticancer drug perfusion for 144 times,64 cases presented changes of electrocardiograms (44.5%),and the incidence rate of arrhythmia was 58.1% in group A.The difference between the two groups was statistically significant (P<0.01).The incidence of arrhythmia in patients aged above or equal to 60 was higher than that aged between 50 and 59 (P<0.05).Conclusions: Regarding to the two methods of interventional chemotherapy,the incidence of arrhythmia is higher in Group A.That implies that the cardiac toxicity initiated by the method used in Group A is not different from that initiated by the method of intravenous chemotherapy,and such effect occurs frequently among the elderly,which should be highly alerted to in the process of interventional chemotherapy.
2010, 35(8): 837-838.
Abstract:
Objective: To evaluate teaching efficacy of multimedia courseware for the operational skills of otolaryngology nursing practice and the teaching effectiveness.Methods: The reformed multimedia courseware for teaching and learning was implemented in Nursing Department of 2004 undergraduates,and compared to the traditional teaching of the 2003 undergraduate.The characteristics of the courseware and teaching effectiveness were analyzed.Results: The average score of otolaryngology nursing technical operations in 2004 nursing undergraduates (95.4 points) was higher than that in 2003 nursing undergraduates (87.6 points),clinical assessment was better than in the past.Conclusions: This multimedia courseware is an ideal modern teaching aid for otolaryngology teaching.
Objective: To evaluate teaching efficacy of multimedia courseware for the operational skills of otolaryngology nursing practice and the teaching effectiveness.Methods: The reformed multimedia courseware for teaching and learning was implemented in Nursing Department of 2004 undergraduates,and compared to the traditional teaching of the 2003 undergraduate.The characteristics of the courseware and teaching effectiveness were analyzed.Results: The average score of otolaryngology nursing technical operations in 2004 nursing undergraduates (95.4 points) was higher than that in 2003 nursing undergraduates (87.6 points),clinical assessment was better than in the past.Conclusions: This multimedia courseware is an ideal modern teaching aid for otolaryngology teaching.
2010, 35(8): 839-840.
Abstract:
Objective: To explore the application of the three dimensional rapid-prototyping (RP) of complex orbital fracture model in plastic surgery teaching effectiveness.Methods: Using the data of head CT three-dimensional reconstructiona and RP technology,orbital fracture model was produced for clinical and theoretical teaching of trauma.Results: Students had a preliminary understanding of the complexity of orbital fracture,and a further understanding of orbital fracture diagnosis and treatment methods.Conclusions: Through the three dimensional rapid-prototyping,the students can visually aware of the orbital fractures in the clinical teaching,and improve the ability of diagnosis and treatment on orbital fractures.
Objective: To explore the application of the three dimensional rapid-prototyping (RP) of complex orbital fracture model in plastic surgery teaching effectiveness.Methods: Using the data of head CT three-dimensional reconstructiona and RP technology,orbital fracture model was produced for clinical and theoretical teaching of trauma.Results: Students had a preliminary understanding of the complexity of orbital fracture,and a further understanding of orbital fracture diagnosis and treatment methods.Conclusions: Through the three dimensional rapid-prototyping,the students can visually aware of the orbital fractures in the clinical teaching,and improve the ability of diagnosis and treatment on orbital fractures.
2010, 35(8): 843-844.
Abstract:
Objective: To evaluate the value of the three-class nursing responsibility system in improving the quality of the clinical care.Methods: According to the general situation of hospital nursing staff,Department of Nursing will set management mode in order to analyze and make a comparision between the nursing quality before and after the application of three-class nursing responsibility system.Results: Indexes of clinical nursing quality and degree of patients' satisfaction have been improved significantly (P<0.01).Conclusions: Three-class nursing responsibility system makes reasonable use of nursing staffing,which is an effective means to improve the quality of clinical care.
Objective: To evaluate the value of the three-class nursing responsibility system in improving the quality of the clinical care.Methods: According to the general situation of hospital nursing staff,Department of Nursing will set management mode in order to analyze and make a comparision between the nursing quality before and after the application of three-class nursing responsibility system.Results: Indexes of clinical nursing quality and degree of patients' satisfaction have been improved significantly (P<0.01).Conclusions: Three-class nursing responsibility system makes reasonable use of nursing staffing,which is an effective means to improve the quality of clinical care.
2010, 35(8): 845-846.
Abstract:
Objective: To summarize the nursing experience of the patients with midst-severe craniocerebral injury accepting hyperbaric oxygen therapy.Methods: Forty-two midst-severe craniocerebral injury cases were treated with hyperbaric oxygen therapy,and another 42 cases of patients with the same type were treated with conventional therapy as the control group,the efficacy of the two groups was compared.Results: In hyperbaric oxygen therapy group,there was no case of therapy induced injury,its efficacy was significantly better than in control group (P<0.05).Conclusions: For midst-severe craniocerebral injury patients treated with hyperbaric oxygen therapy,it is required of nursing care seriously,prevention complications happening,so as to enhance therapeutic effect.
Objective: To summarize the nursing experience of the patients with midst-severe craniocerebral injury accepting hyperbaric oxygen therapy.Methods: Forty-two midst-severe craniocerebral injury cases were treated with hyperbaric oxygen therapy,and another 42 cases of patients with the same type were treated with conventional therapy as the control group,the efficacy of the two groups was compared.Results: In hyperbaric oxygen therapy group,there was no case of therapy induced injury,its efficacy was significantly better than in control group (P<0.05).Conclusions: For midst-severe craniocerebral injury patients treated with hyperbaric oxygen therapy,it is required of nursing care seriously,prevention complications happening,so as to enhance therapeutic effect.
2010, 35(8): 847-849.
Abstract:
Objective: To explore the clinical value of clinical nursing pathway (CNP) for patients with laparoscopic resection in the treatment of ovarian cyst.Methods: Sixty-six cases of ovarian cyst with laparoscopic resection operation were divided into two groups:33 cases in observational group with CNP and 33 cases in control group with traditional model of medical care.The nursing indexes were compared between the two groups.Results: There were significantly differences between the two groups in these aspects including hospital stay,hospital costs,degrees of satisfaction and health knowledge of situation (P<0.05-P<0.01).Conclusions: CNP for patients with laparoscopic resection in the treatment of ovarian cyst can improve both the quality and efficiency of nursing.It's worth spreading in clinical application.
Objective: To explore the clinical value of clinical nursing pathway (CNP) for patients with laparoscopic resection in the treatment of ovarian cyst.Methods: Sixty-six cases of ovarian cyst with laparoscopic resection operation were divided into two groups:33 cases in observational group with CNP and 33 cases in control group with traditional model of medical care.The nursing indexes were compared between the two groups.Results: There were significantly differences between the two groups in these aspects including hospital stay,hospital costs,degrees of satisfaction and health knowledge of situation (P<0.05-P<0.01).Conclusions: CNP for patients with laparoscopic resection in the treatment of ovarian cyst can improve both the quality and efficiency of nursing.It's worth spreading in clinical application.
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