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2012 Vol. 37, No. 9
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2012, 36(9): 1009-1012.
Abstract:
Objective: To observe the proportions of TH17-like cells and TH22-like cells in T cells and T cells in healthy adult peripheral blood.Methods: Eighteen healthy adult peripheral blood samples were stained with fluorescent antibody and the percentages of interleukin-22， interleukin-17 and IFN--producing cells in T cells and T cells were detected by flow cytometry.Results: The CD3 + T cells in the whole blood cells were divided into two groups: T cells and T cells.The proportions of TH1-like cells，TH17- like cells and TH22-like cells in T cells were 14.31%，1 .16% and 1.06%， respectively.While the proportions of TH1-like cells， TH17-like cells and TH22-like cells in T cells were 61.07%，0.27% and 0.91%， respectively.The proportion of TH1-like cells in T cells was lower than in T cells， and the proportion of TH17-like cells in T cells was higher than in T cells (P0.01) .There were no statistical differences about the proportion of TH22-like cells in T cells and in T cells(P0.05) .Conclusions: There exists IL-17 and IL-22 double-positive T17 cell subpopulation and IL-22 single-positive T22 cell subpopulation in T cells.But the proportions are lower than those in T cells.
Objective: To observe the proportions of TH17-like cells and TH22-like cells in T cells and T cells in healthy adult peripheral blood.Methods: Eighteen healthy adult peripheral blood samples were stained with fluorescent antibody and the percentages of interleukin-22， interleukin-17 and IFN--producing cells in T cells and T cells were detected by flow cytometry.Results: The CD3 + T cells in the whole blood cells were divided into two groups: T cells and T cells.The proportions of TH1-like cells，TH17- like cells and TH22-like cells in T cells were 14.31%，1 .16% and 1.06%， respectively.While the proportions of TH1-like cells， TH17-like cells and TH22-like cells in T cells were 61.07%，0.27% and 0.91%， respectively.The proportion of TH1-like cells in T cells was lower than in T cells， and the proportion of TH17-like cells in T cells was higher than in T cells (P0.01) .There were no statistical differences about the proportion of TH22-like cells in T cells and in T cells(P0.05) .Conclusions: There exists IL-17 and IL-22 double-positive T17 cell subpopulation and IL-22 single-positive T22 cell subpopulation in T cells.But the proportions are lower than those in T cells.
2012, 36(9): 1013-1015.
Abstract:
Objective: To observe the protective effect of diazoxide preconditioning on rat myocardial ischemia-reperfusion injury in vivo and observe the effect of diazoxide preconditioning on connexin 43 (Cx43) .Methods: Forty-five male SD rats were randomly divided into five groups: sham group， IRI group， IPC group，DPC group and 5-HD group，9 rats in each group.Myocardial ischemiareperfusion injury model was established by ligation of left anterior descending coronary artery for 30 minutes， followed by 120 minutes of reperfusion.The left ventricular systolic pressure， left ventricular diastolic pressure and other heart function were measured by Medlab biological signal collecting and processing system.After reperfusion，myocardial enzymes in plasma were tested; and then the expressions of TCx43 and PCx43 of ischemia-reperfusion myocardium were studied by western blot.Results: Compared with IRI group， the levels of CK-MB and LDH were decreased with significant statistical difference in DPC group(P0.05-P0.01) ; the expression level of Cx43 protein was significantly higher， especially of PCx43(P0.01) ; and left ventricular systolic and diastolic function were increased (P0.05-P0.01) .Conclusions: Diazoxide preconditioning can effectively retain the expression level of Cx43 of left ventricular myocardium caused by ischemia-reperfusion， reduce myocardial injury， and maintain systolic and diastolic function of left ventricle.Cx43 may be involved in the protective effect of diazoxide preconditioning.
Objective: To observe the protective effect of diazoxide preconditioning on rat myocardial ischemia-reperfusion injury in vivo and observe the effect of diazoxide preconditioning on connexin 43 (Cx43) .Methods: Forty-five male SD rats were randomly divided into five groups: sham group， IRI group， IPC group，DPC group and 5-HD group，9 rats in each group.Myocardial ischemiareperfusion injury model was established by ligation of left anterior descending coronary artery for 30 minutes， followed by 120 minutes of reperfusion.The left ventricular systolic pressure， left ventricular diastolic pressure and other heart function were measured by Medlab biological signal collecting and processing system.After reperfusion，myocardial enzymes in plasma were tested; and then the expressions of TCx43 and PCx43 of ischemia-reperfusion myocardium were studied by western blot.Results: Compared with IRI group， the levels of CK-MB and LDH were decreased with significant statistical difference in DPC group(P0.05-P0.01) ; the expression level of Cx43 protein was significantly higher， especially of PCx43(P0.01) ; and left ventricular systolic and diastolic function were increased (P0.05-P0.01) .Conclusions: Diazoxide preconditioning can effectively retain the expression level of Cx43 of left ventricular myocardium caused by ischemia-reperfusion， reduce myocardial injury， and maintain systolic and diastolic function of left ventricle.Cx43 may be involved in the protective effect of diazoxide preconditioning.
2012, 36(9): 1016-1019,1023.
Abstract:
Objective: To observe the protective effect of diazoxide preconditioning on rat myocardial ischemia-reperfusion injury in vivo and observe the effect of diazoxide preconditioning on connexin 43 (Cx43) .Methods: Forty-five male SD rats were randomly divided into five groups: sham group， IRI group， IPC group，DPC group and 5-HD group，9 rats in each group.Myocardial ischemiareperfusion injury model was established by ligation of left anterior descending coronary artery for 30 minutes， followed by 120 minutes of reperfusion.The left ventricular systolic pressure， left ventricular diastolic pressure and other heart function were measured by Medlab biological signal collecting and processing system.After reperfusion，myocardial enzymes in plasma were tested; and then the expressions of TCx43 and PCx43 of ischemia-reperfusion myocardium were studied by western blot.Results: Compared with IRI group， the levels of CK-MB and LDH were decreased with significant statistical difference in DPC group(P0.05-P0.01) ; the expression level of Cx43 protein was significantly higher， especially of PCx43(P0.01) ; and left ventricular systolic and diastolic function were increased (P0.05-P0.01) .Conclusions: Diazoxide preconditioning can effectively retain the expression level of Cx43 of left ventricular myocardium caused by ischemia-reperfusion， reduce myocardial injury， and maintain systolic and diastolic function of left ventricle.Cx43 may be involved in the protective effect of diazoxide preconditioning.
Objective: To observe the protective effect of diazoxide preconditioning on rat myocardial ischemia-reperfusion injury in vivo and observe the effect of diazoxide preconditioning on connexin 43 (Cx43) .Methods: Forty-five male SD rats were randomly divided into five groups: sham group， IRI group， IPC group，DPC group and 5-HD group，9 rats in each group.Myocardial ischemiareperfusion injury model was established by ligation of left anterior descending coronary artery for 30 minutes， followed by 120 minutes of reperfusion.The left ventricular systolic pressure， left ventricular diastolic pressure and other heart function were measured by Medlab biological signal collecting and processing system.After reperfusion，myocardial enzymes in plasma were tested; and then the expressions of TCx43 and PCx43 of ischemia-reperfusion myocardium were studied by western blot.Results: Compared with IRI group， the levels of CK-MB and LDH were decreased with significant statistical difference in DPC group(P0.05-P0.01) ; the expression level of Cx43 protein was significantly higher， especially of PCx43(P0.01) ; and left ventricular systolic and diastolic function were increased (P0.05-P0.01) .Conclusions: Diazoxide preconditioning can effectively retain the expression level of Cx43 of left ventricular myocardium caused by ischemia-reperfusion， reduce myocardial injury， and maintain systolic and diastolic function of left ventricle.Cx43 may be involved in the protective effect of diazoxide preconditioning.
Expressions of transforming growth factor-1 and connective tissue growth factor in viral myocarditis
2012, 36(9): 1020-1023.
Abstract:
Objective: To investigate the expressions of transforming growth factor-1(TGF-1) and connective tissue growth factor (CTGF) in viral myocarditis.Methods: Thirty-six male Balb /c mice were divided into two groups randomly: control group and model group.Model group was established by Cox-sackie virus B3 induced viral myocarditis.Six mice in each group were killed on days 7， 14 and 28 respectively.The hearts were weighed and fixed with 4% paraform，histological cross sections were stained with hematoxylineosin and myocardial histopathoplogic scores were counted under optical microscope.The protein expressions of myocardial collagen Ⅰ and Ⅲ were examined by immunohistochemical method and analyzed using a computer assisted procedure， then the area of collagens were calculated.The protein expressions of TGF-1 and CTGF were also examined by immunohistochemical method， and the absorbance and area of positive cells were measured after standard gray correction， the antigen contents were calculated by product of absorbance and area.Results: In contrast to control group， the histopathoplogic scores， expressions of myocardial collagen Ⅰ and Ⅲ，TGF-1 and CTGF were increased markedly in Cox-sackie virus B3 infected group (P0.01) .The expression of myocardial collagen Ⅲ was correlated with TGF-1 and CTGF positively (P0.05) ， the expression of TGF-1 was correlated with CTGF positively (P0.05) .Conclusions: TGF-1 and CTGF participate in the occurreuce and development of myocardial fibrosis of viral myocarditis.
Objective: To investigate the expressions of transforming growth factor-1(TGF-1) and connective tissue growth factor (CTGF) in viral myocarditis.Methods: Thirty-six male Balb /c mice were divided into two groups randomly: control group and model group.Model group was established by Cox-sackie virus B3 induced viral myocarditis.Six mice in each group were killed on days 7， 14 and 28 respectively.The hearts were weighed and fixed with 4% paraform，histological cross sections were stained with hematoxylineosin and myocardial histopathoplogic scores were counted under optical microscope.The protein expressions of myocardial collagen Ⅰ and Ⅲ were examined by immunohistochemical method and analyzed using a computer assisted procedure， then the area of collagens were calculated.The protein expressions of TGF-1 and CTGF were also examined by immunohistochemical method， and the absorbance and area of positive cells were measured after standard gray correction， the antigen contents were calculated by product of absorbance and area.Results: In contrast to control group， the histopathoplogic scores， expressions of myocardial collagen Ⅰ and Ⅲ，TGF-1 and CTGF were increased markedly in Cox-sackie virus B3 infected group (P0.01) .The expression of myocardial collagen Ⅲ was correlated with TGF-1 and CTGF positively (P0.05) ， the expression of TGF-1 was correlated with CTGF positively (P0.05) .Conclusions: TGF-1 and CTGF participate in the occurreuce and development of myocardial fibrosis of viral myocarditis.
2012, 36(9): 1024-1026.
Abstract:
Objective: To observe the expression of 5-Hydroxytryptamine(5-HT) and the distinction of its distribution in the inferior culliculus in young， adult and old rats(3， 12 and 24 months old) and to explore the possibly physiological cause as well as the effect.Methods: The expressions of 5-HT among young rats， adult rats and old rats were examined by immunohistochemistry.Results: 5-HT immunoreactive cells and fibers were widely distributed in some regions of inferior culliculus 5-HT immunoreactive cells appeared mainly in inferior culliculus central(ICC) ，while the stronger labelled fibers mainly in ICC ventral.The old rats exhibited less in the number and weaker in immunoreaction than that in young adult rats (P0.01 ) .Conclusions: The expression of 5-HT showed significantly age-related changes，which were related to the functional changes of synthesis， re-incept as well as metabolizability，and probably affect the modulation to the information of auditory input in the inferior culliculus during aging.
Objective: To observe the expression of 5-Hydroxytryptamine(5-HT) and the distinction of its distribution in the inferior culliculus in young， adult and old rats(3， 12 and 24 months old) and to explore the possibly physiological cause as well as the effect.Methods: The expressions of 5-HT among young rats， adult rats and old rats were examined by immunohistochemistry.Results: 5-HT immunoreactive cells and fibers were widely distributed in some regions of inferior culliculus 5-HT immunoreactive cells appeared mainly in inferior culliculus central(ICC) ，while the stronger labelled fibers mainly in ICC ventral.The old rats exhibited less in the number and weaker in immunoreaction than that in young adult rats (P0.01 ) .Conclusions: The expression of 5-HT showed significantly age-related changes，which were related to the functional changes of synthesis， re-incept as well as metabolizability，and probably affect the modulation to the information of auditory input in the inferior culliculus during aging.
2012, 36(9): 1027-1028.
Abstract:
Objective: To investigate the efficacy of intensive insulin therapy in treatment of stress hyperglycemia caused by acute cerebral stroke.Methods: One hundred and sixty nondiabete cases with stress hyperglycaemia caused by acute cerebral stroke were randomly divided into the observation group and control group(80 cases each group) .The observation group were treated with intensive insulin and the levels of blood glucose were controlled at the range of 4.4 to 6.1 mmol /L.The contol group were treated with conventional insulin when the levels of blood glucose were greater than or equal to 11.1 mmol /L and the levels of blood glucose were controlled at the range of 8.0 to 11.1 mmol /L.The mortality， the incidence of infection complication and the neural function recovery of two groups were observed.Results: The incidence of pulmonary and urinary tract infection and mortality of the observation group were 8.75% and 6.25%，and 2.50%， respectively，which were significantly lower than that of the control group(P0.01) .The nerve function scores of the observation group were better than that of the control group on the 15 and 30 days in hospital(P0.01) .Conclusions: Intensive insulin therapy can decrease the mortality and the incidence of infection of acute cerebral stroke complicated with stress hyperglycaemia and promote neural function recovery.
Objective: To investigate the efficacy of intensive insulin therapy in treatment of stress hyperglycemia caused by acute cerebral stroke.Methods: One hundred and sixty nondiabete cases with stress hyperglycaemia caused by acute cerebral stroke were randomly divided into the observation group and control group(80 cases each group) .The observation group were treated with intensive insulin and the levels of blood glucose were controlled at the range of 4.4 to 6.1 mmol /L.The contol group were treated with conventional insulin when the levels of blood glucose were greater than or equal to 11.1 mmol /L and the levels of blood glucose were controlled at the range of 8.0 to 11.1 mmol /L.The mortality， the incidence of infection complication and the neural function recovery of two groups were observed.Results: The incidence of pulmonary and urinary tract infection and mortality of the observation group were 8.75% and 6.25%，and 2.50%， respectively，which were significantly lower than that of the control group(P0.01) .The nerve function scores of the observation group were better than that of the control group on the 15 and 30 days in hospital(P0.01) .Conclusions: Intensive insulin therapy can decrease the mortality and the incidence of infection of acute cerebral stroke complicated with stress hyperglycaemia and promote neural function recovery.
2012, 36(9): 1029-1030,1033.
Abstract:
Objective: To investigate the relationship between pancreatic leakage and pancreaticojejunostomy of pancreatico- jejunal postoperation and search the effective operation to prevent the pancreatic leakage.Methods: One hundred and fourteen patients were operated using pancreaticodudenectomy including 48 cases with pancreatico-jejunal ene-to-end binding，37 cases with end-to-side anastomosis and 29 cases with pancreatico-jejunal sleeve anastomosis.Results: Twenty cases with pancreatic leakage were detected， which accounted for 17.5% including 8 cases in pancreatico-jejunal ene-to-end binding，5 cases in end-to-side anastomosis and 7 cases in pancreatico-jejunal sleeve anastomosis.The incidence differences of pancreatic leakage of three types of pancreaticojejunostomy were not statistical significant(P0.05) .Conclusions: The effects of three types of pancreaticojejunostomy are not different and the skilled operation technology can obviously reduce the incidence of pancreatic leakage.
Objective: To investigate the relationship between pancreatic leakage and pancreaticojejunostomy of pancreatico- jejunal postoperation and search the effective operation to prevent the pancreatic leakage.Methods: One hundred and fourteen patients were operated using pancreaticodudenectomy including 48 cases with pancreatico-jejunal ene-to-end binding，37 cases with end-to-side anastomosis and 29 cases with pancreatico-jejunal sleeve anastomosis.Results: Twenty cases with pancreatic leakage were detected， which accounted for 17.5% including 8 cases in pancreatico-jejunal ene-to-end binding，5 cases in end-to-side anastomosis and 7 cases in pancreatico-jejunal sleeve anastomosis.The incidence differences of pancreatic leakage of three types of pancreaticojejunostomy were not statistical significant(P0.05) .Conclusions: The effects of three types of pancreaticojejunostomy are not different and the skilled operation technology can obviously reduce the incidence of pancreatic leakage.
2012, 36(9): 1031-1033.
Abstract:
Objective: To analyze the clinical significance of detection of des--carboxyprohrombin(DCP) ，-fetoprotein-L3 (AFPL3%) and -fetoprotein(AFP) alone or in combination for diagnosis of hepatocellular carcinoma(HCC) .Methods: The serum levels of those three biomarkers were detected by ELISA in patients with HCC， cirrhosis and chronic hepatis B and compared with each other.The diagnostic evaluation of the three tumor biomarkers alone or combined detection was used to analyze the sensitivity， specificity， the area under the curve and Youden index in the diagnosis of HCC.Results: The value of the three biomarkers in HCC patients were higher than those in cirrhosis and chronic hepatis B patients(P0.01) .In the cut-off value of 40 mAU/ml，10% and 20 ng /ml for DCP， AFP-L3% and AFP， the sensitivity and specificity were 87.88% and 95.00%，57.58% and 90.00%，75.76% and 63.33%， respeclively.While when those in combination， the sensitivity and specificity were not better than DCP alone.Conclusions: Whether DCP，AFP-13%，AFP are combined or alone for detection，DCP showes the best sensitivity and specificity for HCC diagnosis， so it can be used as main serum biomarker for diagnosis of HCC.
Objective: To analyze the clinical significance of detection of des--carboxyprohrombin(DCP) ，-fetoprotein-L3 (AFPL3%) and -fetoprotein(AFP) alone or in combination for diagnosis of hepatocellular carcinoma(HCC) .Methods: The serum levels of those three biomarkers were detected by ELISA in patients with HCC， cirrhosis and chronic hepatis B and compared with each other.The diagnostic evaluation of the three tumor biomarkers alone or combined detection was used to analyze the sensitivity， specificity， the area under the curve and Youden index in the diagnosis of HCC.Results: The value of the three biomarkers in HCC patients were higher than those in cirrhosis and chronic hepatis B patients(P0.01) .In the cut-off value of 40 mAU/ml，10% and 20 ng /ml for DCP， AFP-L3% and AFP， the sensitivity and specificity were 87.88% and 95.00%，57.58% and 90.00%，75.76% and 63.33%， respeclively.While when those in combination， the sensitivity and specificity were not better than DCP alone.Conclusions: Whether DCP，AFP-13%，AFP are combined or alone for detection，DCP showes the best sensitivity and specificity for HCC diagnosis， so it can be used as main serum biomarker for diagnosis of HCC.
2012, 36(9): 1034-1036,1039.
Abstract:
Objective: To investigate the feasibility and technique of laparoscopic complex myomectomy.Methods: The clinical data of 22 cases undergone laparoscopic complex myomectomy were retrospectively analyzed， and compared with the data of 36 cases undergone traditional open surgery at the same time.The operation time， intraoperative bleeding，postoperative exhausting time，analgesic using， postoperative morbidity，wound healing，postoperative hospital stays and follow-up were recorded.Results: The operation of 22 cases were successfully completed under laparoscope.Compared with the cases undergone traditional open surgery， the cases undergone laparoscopic myomectomy were less bleeding， fast recovery of intestinal function， less postoperative morbidity and pain， short hospital stays and long operation time(P0.05 toP0.01) .Conclusions: The laparoscopic complex myomectomy is safety， feasible and has good development prospect on the basis of mastering laparoscopic surgical operation and suture techniques.
Objective: To investigate the feasibility and technique of laparoscopic complex myomectomy.Methods: The clinical data of 22 cases undergone laparoscopic complex myomectomy were retrospectively analyzed， and compared with the data of 36 cases undergone traditional open surgery at the same time.The operation time， intraoperative bleeding，postoperative exhausting time，analgesic using， postoperative morbidity，wound healing，postoperative hospital stays and follow-up were recorded.Results: The operation of 22 cases were successfully completed under laparoscope.Compared with the cases undergone traditional open surgery， the cases undergone laparoscopic myomectomy were less bleeding， fast recovery of intestinal function， less postoperative morbidity and pain， short hospital stays and long operation time(P0.05 toP0.01) .Conclusions: The laparoscopic complex myomectomy is safety， feasible and has good development prospect on the basis of mastering laparoscopic surgical operation and suture techniques.
2012, 36(9): 1037-1039.
Abstract:
Objective: To investigate the effects and safety of vinpocetine on patients with vertebro-basilar insufficiency(VBI) .Methods: One hundred and twenty patients with VBI were randomly divided into the theatment group(60 cases) and the control group(60 cases) .The patients in theatment and control groups were injected with 20 mg of vinpocetine and 40 mg of Dengzhanhuasu everyday for 14 days， respectively.Results: The total effective rate of theatment group(93.33%) was obviously higher than that of the control group(70.00%，P0.01) .The index of hemorheology and hemodynamic of two groups were obviously improved after treatment(P0.01) ，and which of the treatment group were better than that of the control group.There were no obvious adverse reations in two groups.Conclusions: The curative effects of vinpocetine on treating VBI are good，which it is worthy of being widely applied in clinic.
Objective: To investigate the effects and safety of vinpocetine on patients with vertebro-basilar insufficiency(VBI) .Methods: One hundred and twenty patients with VBI were randomly divided into the theatment group(60 cases) and the control group(60 cases) .The patients in theatment and control groups were injected with 20 mg of vinpocetine and 40 mg of Dengzhanhuasu everyday for 14 days， respectively.Results: The total effective rate of theatment group(93.33%) was obviously higher than that of the control group(70.00%，P0.01) .The index of hemorheology and hemodynamic of two groups were obviously improved after treatment(P0.01) ，and which of the treatment group were better than that of the control group.There were no obvious adverse reations in two groups.Conclusions: The curative effects of vinpocetine on treating VBI are good，which it is worthy of being widely applied in clinic.
2012, 36(9): 1040-1042.
Abstract:
Objective: To explore the value of F waves of the upper and lower limbs in the diagnosis of diabetic peripheral neuropathy (DPN) .Methods: The F waves of the median and tibial nerves of lower limbs and upper limbs of 82 cases with diabetes(D group) and 22 healthy volunteers with age-and height-matched (Control group) were detected.The data of the appearance rate (F present) ， conduction velocity(Fcv) ，mean latency(Flme) of F waves were analyzed.The cases of group D were divided into symptom group and nosymptom according to the peripheral neuropathy symptoms， and normal and abnormal group according to nerve conduction detection (NCD) .Results: Compared with the control group， group D with symptoms had less Fpresent and slower Fcv in upper limbs and less Fpresent and longer Flme in lower limbs(P0.05 toP0.01) ， less Fpresent was only detected in nosymptoms.Compared with group D with nosymptoms， slower Fcv in the upper， and less Fpresent and longer Flme in the lower limbs were detected in symptoms group(P0.05 toP0.01) ， the differences of Fpresent of two groups were not statistical significant(P0.05) .Compared with the control group， the slower Fcv in upper limbs， slower Fcv of upper limbs and the less Fpresent and longer Flme of lower limbs were detected in diabetes with abnormal NCD，while for diabetes with normal NCD， the less Fpresent and slower Fcv in upper limbs and no obvious difference in lower limbs were detected.Compared with diabetes with normal NCD， slower Fcv in upper limbs and slower Fcv and longer Flme of lower limbs were detected in diabetes with abnormal NCD， and no obvious difference in upper limbs were detected(P0.05) .Conclusions: F wave(especially Fpresent) of upper limbs can be used as a sensitive diagnostic means for earlier DPN，and discover subclinical lesions.The proximal nerve damage of the upper limbs occurs sooner than the distal end.The parameter variation of the F waves are consistent to the clinical symptoms and NCD.Both proximal and distal nerves of the lower limbs can be involved synchronally in the DPN.
Objective: To explore the value of F waves of the upper and lower limbs in the diagnosis of diabetic peripheral neuropathy (DPN) .Methods: The F waves of the median and tibial nerves of lower limbs and upper limbs of 82 cases with diabetes(D group) and 22 healthy volunteers with age-and height-matched (Control group) were detected.The data of the appearance rate (F present) ， conduction velocity(Fcv) ，mean latency(Flme) of F waves were analyzed.The cases of group D were divided into symptom group and nosymptom according to the peripheral neuropathy symptoms， and normal and abnormal group according to nerve conduction detection (NCD) .Results: Compared with the control group， group D with symptoms had less Fpresent and slower Fcv in upper limbs and less Fpresent and longer Flme in lower limbs(P0.05 toP0.01) ， less Fpresent was only detected in nosymptoms.Compared with group D with nosymptoms， slower Fcv in the upper， and less Fpresent and longer Flme in the lower limbs were detected in symptoms group(P0.05 toP0.01) ， the differences of Fpresent of two groups were not statistical significant(P0.05) .Compared with the control group， the slower Fcv in upper limbs， slower Fcv of upper limbs and the less Fpresent and longer Flme of lower limbs were detected in diabetes with abnormal NCD，while for diabetes with normal NCD， the less Fpresent and slower Fcv in upper limbs and no obvious difference in lower limbs were detected.Compared with diabetes with normal NCD， slower Fcv in upper limbs and slower Fcv and longer Flme of lower limbs were detected in diabetes with abnormal NCD， and no obvious difference in upper limbs were detected(P0.05) .Conclusions: F wave(especially Fpresent) of upper limbs can be used as a sensitive diagnostic means for earlier DPN，and discover subclinical lesions.The proximal nerve damage of the upper limbs occurs sooner than the distal end.The parameter variation of the F waves are consistent to the clinical symptoms and NCD.Both proximal and distal nerves of the lower limbs can be involved synchronally in the DPN.
2012, 36(9): 1043-1044.
Abstract:
Objective: To compare the effects of ibutilide and amiodarone on the atrial flutter (AFL) or atrial fihllation (AF) .Methods: Twenty-seven cases with AF of AFL were randomly divided into ibutilide group(13 cases) which were injected with 1 mg ibutilide within 10 min and added 1mg if still invalid after 10 mins， and amiodarone group(14 cases) which were injected with 150 mg amiodarone within 15 mins and added 75 mg if still invalid after 10 mins.Results: The conversing time of AF or AFL of ibutilide group was shorter than that of amiodarone group(P0.05) .Eleven cases of ibutilide group and 6 cases of amiodarone group conversed sinus rhythm with 30 mins after tretament， and 3 cases and 2 cases respectively in 30 to 90 mins after treatment.The total conversing rates of two groups were 100% and 61.54% respectively and their differences was not statistically significant(P = 0.167) .Conclusions: The conversing effects of ibutilide on AF or AFL is better than that of amiodarone and the coversed time is also shorter.
Objective: To compare the effects of ibutilide and amiodarone on the atrial flutter (AFL) or atrial fihllation (AF) .Methods: Twenty-seven cases with AF of AFL were randomly divided into ibutilide group(13 cases) which were injected with 1 mg ibutilide within 10 min and added 1mg if still invalid after 10 mins， and amiodarone group(14 cases) which were injected with 150 mg amiodarone within 15 mins and added 75 mg if still invalid after 10 mins.Results: The conversing time of AF or AFL of ibutilide group was shorter than that of amiodarone group(P0.05) .Eleven cases of ibutilide group and 6 cases of amiodarone group conversed sinus rhythm with 30 mins after tretament， and 3 cases and 2 cases respectively in 30 to 90 mins after treatment.The total conversing rates of two groups were 100% and 61.54% respectively and their differences was not statistically significant(P = 0.167) .Conclusions: The conversing effects of ibutilide on AF or AFL is better than that of amiodarone and the coversed time is also shorter.
2012, 36(9): 1045-1048.
Abstract:
Objective: To explore the roles of transforming growth factor 1(TGF1) ，TGFRⅠ and TGFRⅡ in the development of the large intestine cancer.Methods: The expressions of TGF1，TGFRⅠ and TGFRⅡ of colorectal adenoma of 50 cases， large intestine cancer of 40 cases and normal tissue of 20 cases were detected through immunohistochemistry.Results: The expressions of TGF1 and TGFRⅡ of the three groups were statistical significant(P0.01) ，but not different for TGFRⅠ(P0.05) .The expression of TGF1 in colorectal adenoma and large intestine cancer with Duke's A stage was statistical significant(P0.05) ，but not different for TGFRⅠ and TGFRⅡ(P0.05) .Conclusions: The expressions of TGF1 and TGFRⅡ in normal tissue， colorectal adenoma and large intestine cancer are different，which demonstrate that the expression may participate the forming of large intestine cancer， and TGF1 may play some roles in the early stage of colorectal adenoma-carcinoma transition.
Objective: To explore the roles of transforming growth factor 1(TGF1) ，TGFRⅠ and TGFRⅡ in the development of the large intestine cancer.Methods: The expressions of TGF1，TGFRⅠ and TGFRⅡ of colorectal adenoma of 50 cases， large intestine cancer of 40 cases and normal tissue of 20 cases were detected through immunohistochemistry.Results: The expressions of TGF1 and TGFRⅡ of the three groups were statistical significant(P0.01) ，but not different for TGFRⅠ(P0.05) .The expression of TGF1 in colorectal adenoma and large intestine cancer with Duke's A stage was statistical significant(P0.05) ，but not different for TGFRⅠ and TGFRⅡ(P0.05) .Conclusions: The expressions of TGF1 and TGFRⅡ in normal tissue， colorectal adenoma and large intestine cancer are different，which demonstrate that the expression may participate the forming of large intestine cancer， and TGF1 may play some roles in the early stage of colorectal adenoma-carcinoma transition.
2012, 36(9): 1048-1050.
Abstract:
Objective: To observe the short-term efficacy and toxicity of paclitxel plus cisplatin combined with radiotherapy in treatment of advanced cervix cancer.Methods: Thirty-six patients with advanced cervix cancer (Ⅱ b-Ⅲ b ) received concurrent chemoradiotherapy.The external irradiation dose of radiotherapy was 2Gy per time，5d /W，with a total dose of 50Gy; 192 Ir high-dose-rate afterloading unit was adopted for branchytherapy one week after external radiotherapy， at a dose of 7 Gy per time in A point for 6 times.Paclitxel (135mg /m2 ，d1 ) and cisplatin (25mg /m2 ，d1- d3 ) were given every 3- 4 weeks for two cycles concurrently with radiotherapy.The short effectiveness and toxic reaction were observed in these patients.Results: Of the 36 cases， complete response was observed in 27 cases，partial response in 7 cases and two cases presented certain progress.The overall response rate was 94.4%.There was no statistical significance in differences of patients' pathologic patterns and clinical stages(P0.05) .The main toxicities were leucopenia and radiation rectal reaction.Conclusions: The short term efficacy of concurrent chemoradiotherapy with paclitxel and cisplatin is relatively good on patients with advanced cervical cancer， and the side effects are well tolerated.
Objective: To observe the short-term efficacy and toxicity of paclitxel plus cisplatin combined with radiotherapy in treatment of advanced cervix cancer.Methods: Thirty-six patients with advanced cervix cancer (Ⅱ b-Ⅲ b ) received concurrent chemoradiotherapy.The external irradiation dose of radiotherapy was 2Gy per time，5d /W，with a total dose of 50Gy; 192 Ir high-dose-rate afterloading unit was adopted for branchytherapy one week after external radiotherapy， at a dose of 7 Gy per time in A point for 6 times.Paclitxel (135mg /m2 ，d1 ) and cisplatin (25mg /m2 ，d1- d3 ) were given every 3- 4 weeks for two cycles concurrently with radiotherapy.The short effectiveness and toxic reaction were observed in these patients.Results: Of the 36 cases， complete response was observed in 27 cases，partial response in 7 cases and two cases presented certain progress.The overall response rate was 94.4%.There was no statistical significance in differences of patients' pathologic patterns and clinical stages(P0.05) .The main toxicities were leucopenia and radiation rectal reaction.Conclusions: The short term efficacy of concurrent chemoradiotherapy with paclitxel and cisplatin is relatively good on patients with advanced cervical cancer， and the side effects are well tolerated.
2012, 36(9): 1051-1052.
Abstract:
Objective: To investigate the clinical effects and toxic reaction of idarubicin(IDA) on the acute myeloid leukemia(AML) .Methods: Thirty-nine patients with AML were randomly divided into treatment group，which were treated with IDA and Ara-C(23 cases) and control group which were treated with daunorubicin combined with Ara-C(16 cases) .The remission rates and toxic reaction of two groups were observed.Results: The complete remission， total effective and severe infection rates of the treatment and control group were 82.6%and 43.8%， 91.3% and 62.5% and 40.0% and 41.7%， respectively.The difference of total effective of two groups was statistical significant(P0.05) ，but for not severe infection rates(P0.05) .Conclusions: The effects of treatment of AML with chemotherapeutic scheme containing IDA are good.
Objective: To investigate the clinical effects and toxic reaction of idarubicin(IDA) on the acute myeloid leukemia(AML) .Methods: Thirty-nine patients with AML were randomly divided into treatment group，which were treated with IDA and Ara-C(23 cases) and control group which were treated with daunorubicin combined with Ara-C(16 cases) .The remission rates and toxic reaction of two groups were observed.Results: The complete remission， total effective and severe infection rates of the treatment and control group were 82.6%and 43.8%， 91.3% and 62.5% and 40.0% and 41.7%， respectively.The difference of total effective of two groups was statistical significant(P0.05) ，but for not severe infection rates(P0.05) .Conclusions: The effects of treatment of AML with chemotherapeutic scheme containing IDA are good.
2012, 36(9): 1053-1055,1058.
Abstract:
Objective: To investigate the relationship between the left atrium remodeling， von Willebrand factor(vWF) ，D-dimer(DD) ，platelet membrane glycoprotein Ⅱb /Ⅲa(GP Ⅱb /Ⅲa) and left atrial thrombosis， and to explore the mechanisms of thrombosis.Methods: One hundred cases were divided into atrial fibrillation group(60 cases) ， sinus rhythm group(20 cases) and normal control group(20 cases) .Fourty-one cases of atrial fibrillation group were subdivided into thrombosis positive group(21 cases) and thrombosis negative group (20 cases) according to the results of esophageal echocardiography.All cases were detected by transthoraial and transesophageal echocardiography(TTE) .The data of left atrial diameter(LA-D) and thrombosis of left atrium/left atrial appendage were observed.The levels of DD and vWF and GP Ⅱb /Ⅲa were measured using an enzyme linked immunosorbent assay(ELISA) and flow cytometry.Results: The levels of LA-D，vWF，D-D and GP Ⅱb /Ⅲa of sinus rhythm group， thrombosis negative group and thrombosis positive group were increased(P0.01) .The levels of LA-D、vWF、D-D and GP Ⅱb /Ⅲa of the atrial fibrillation group were significantly higher than that of sinus rhythm and normal control group(P0.01) ，while for the later two groups no significant difference(P0.05) .Conclusions: The atrium remodeling，endothelium damage，platelet activation and thrombin increasing may participate in the thrombosis of left atrium.
Objective: To investigate the relationship between the left atrium remodeling， von Willebrand factor(vWF) ，D-dimer(DD) ，platelet membrane glycoprotein Ⅱb /Ⅲa(GP Ⅱb /Ⅲa) and left atrial thrombosis， and to explore the mechanisms of thrombosis.Methods: One hundred cases were divided into atrial fibrillation group(60 cases) ， sinus rhythm group(20 cases) and normal control group(20 cases) .Fourty-one cases of atrial fibrillation group were subdivided into thrombosis positive group(21 cases) and thrombosis negative group (20 cases) according to the results of esophageal echocardiography.All cases were detected by transthoraial and transesophageal echocardiography(TTE) .The data of left atrial diameter(LA-D) and thrombosis of left atrium/left atrial appendage were observed.The levels of DD and vWF and GP Ⅱb /Ⅲa were measured using an enzyme linked immunosorbent assay(ELISA) and flow cytometry.Results: The levels of LA-D，vWF，D-D and GP Ⅱb /Ⅲa of sinus rhythm group， thrombosis negative group and thrombosis positive group were increased(P0.01) .The levels of LA-D、vWF、D-D and GP Ⅱb /Ⅲa of the atrial fibrillation group were significantly higher than that of sinus rhythm and normal control group(P0.01) ，while for the later two groups no significant difference(P0.05) .Conclusions: The atrium remodeling，endothelium damage，platelet activation and thrombin increasing may participate in the thrombosis of left atrium.
2012, 36(9): 1056-1058.
Abstract:
Objective: To evaluate the curative effects of 192Ir afterloading brachytherapy combined with external beam radiation therapy(EBRT) in treatment of stage Ⅱor Ⅲ cervical carcinoma.Methods: One hundred twenty-three patients with stage Ⅱ ～ Ⅲ (FIGO) cervical carcinoma confirmed by pathology were treated with HDR 192 Ir afterloading brachytherapy combined with EBRT.The front and rear pelvic cavity was irradiated at the dose of 1.8 to 2 Gy everytime， five times per week.Pelvic plane center was treated with the dose of 30 to 32 Gy for 3 to 3.5 weeks.The 3 cms lead was placed among pelvic cavity， the dose of 20 to 24 Gy for 2.5 to 3.5 weeks was added to near the uterus， and brachytherapy was employed with the dose of 18 to 24 Gy for 3 to 4 times and lasting 2.5 to 3.5 weeks.The dose of 12 to 18 Gy for 2 to 3 times and lasting 2.5 to 3.5 weeks was applied.The total doses of brachytherapy were 30 to 42 Gy for 5 to 6.5 weeks(point A) .Results: All patients were followed up over 5 years， the survival rates were 62.6%.The 5-year survival rates of stage Ⅱand stage Ⅲ were 68% and 45.8%， respectively.When RTOG/EROTC was more than or equal to 2， the injury incidence of rectum，bladder and rectosigmoid were 22 patients，8 patients and 40 patients， respectively.The incidence of rectovaginal fistula was 2 patients.Conclusions: The curative effects of stage Ⅱor Ⅲ cervical carcinoma treated with 192 Ir afterloading brachytherapy combined with EBRT are good， convenient and high incidence of rectum injury.The scheme of near distance treatment as a whole is safe.
Objective: To evaluate the curative effects of 192Ir afterloading brachytherapy combined with external beam radiation therapy(EBRT) in treatment of stage Ⅱor Ⅲ cervical carcinoma.Methods: One hundred twenty-three patients with stage Ⅱ ～ Ⅲ (FIGO) cervical carcinoma confirmed by pathology were treated with HDR 192 Ir afterloading brachytherapy combined with EBRT.The front and rear pelvic cavity was irradiated at the dose of 1.8 to 2 Gy everytime， five times per week.Pelvic plane center was treated with the dose of 30 to 32 Gy for 3 to 3.5 weeks.The 3 cms lead was placed among pelvic cavity， the dose of 20 to 24 Gy for 2.5 to 3.5 weeks was added to near the uterus， and brachytherapy was employed with the dose of 18 to 24 Gy for 3 to 4 times and lasting 2.5 to 3.5 weeks.The dose of 12 to 18 Gy for 2 to 3 times and lasting 2.5 to 3.5 weeks was applied.The total doses of brachytherapy were 30 to 42 Gy for 5 to 6.5 weeks(point A) .Results: All patients were followed up over 5 years， the survival rates were 62.6%.The 5-year survival rates of stage Ⅱand stage Ⅲ were 68% and 45.8%， respectively.When RTOG/EROTC was more than or equal to 2， the injury incidence of rectum，bladder and rectosigmoid were 22 patients，8 patients and 40 patients， respectively.The incidence of rectovaginal fistula was 2 patients.Conclusions: The curative effects of stage Ⅱor Ⅲ cervical carcinoma treated with 192 Ir afterloading brachytherapy combined with EBRT are good， convenient and high incidence of rectum injury.The scheme of near distance treatment as a whole is safe.
2012, 36(9): 1059-1061.
Abstract:
Objective: To explore the safety and effectiveness of tirofiban in treatment of acute coronary syndrome(ACS) .Methods: Eighty patients with ACS were divided into observation group and control group(40 cases each group) .The routine treatments were performed for the patients in control group，while tirofiban was administered additionally to the patients in observation group.Cardiac troponin T(cTnT) peak level in 24 hour was compared，major adverse cardiac events in 30d， rate of fall of ST segment， left ventricular ejection fraction， adverse reactions and bleeding condition were observed in two groups.Results: There was no bleeding even slight bleeding or thrombocytopenia in either case， and there was no difference of the occurrence rate of bleeding in skin and mucosa between the observation group and control group(20.0% vs.17.5%) (P0.05) .The cTnT peak level in 24 hours in observation group was obviously lower than that in control group，(1.47 0.58) g /L vs.(2.34 0.49) g /L(P0.05) .There was no difference about the occurrence rates of major adverse cardiac events in 30d in both groups(2.5% vs.12.5%) (P0.05) .The rate of fall of ST segment (80.0%) and left ventricular ejection fraction(57.8 6.7) % in observation group were significantly better than those in control group， 60.0% and (45.4 4.8 ) %，(P0.05 ) .Conclusions: For ACS patients， tirofiban based on the routine treatment can effectively decrease the incidence of myocardial cell injury， and it can improve cardiac function.There is a tendency that tirofiban can also decrease the major adverse cardiac events.Besides， its safety is better.
Objective: To explore the safety and effectiveness of tirofiban in treatment of acute coronary syndrome(ACS) .Methods: Eighty patients with ACS were divided into observation group and control group(40 cases each group) .The routine treatments were performed for the patients in control group，while tirofiban was administered additionally to the patients in observation group.Cardiac troponin T(cTnT) peak level in 24 hour was compared，major adverse cardiac events in 30d， rate of fall of ST segment， left ventricular ejection fraction， adverse reactions and bleeding condition were observed in two groups.Results: There was no bleeding even slight bleeding or thrombocytopenia in either case， and there was no difference of the occurrence rate of bleeding in skin and mucosa between the observation group and control group(20.0% vs.17.5%) (P0.05) .The cTnT peak level in 24 hours in observation group was obviously lower than that in control group，(1.47 0.58) g /L vs.(2.34 0.49) g /L(P0.05) .There was no difference about the occurrence rates of major adverse cardiac events in 30d in both groups(2.5% vs.12.5%) (P0.05) .The rate of fall of ST segment (80.0%) and left ventricular ejection fraction(57.8 6.7) % in observation group were significantly better than those in control group， 60.0% and (45.4 4.8 ) %，(P0.05 ) .Conclusions: For ACS patients， tirofiban based on the routine treatment can effectively decrease the incidence of myocardial cell injury， and it can improve cardiac function.There is a tendency that tirofiban can also decrease the major adverse cardiac events.Besides， its safety is better.
2012, 36(9): 1062-1063,1066.
Abstract:
Objective: To observe the efficacy and cellular immune function of autologous cytokine induced killer (CIK) cells treatment plus Huisheng oral liquid in patients with advanced esophageal carcinoma.Methods: A total of 62 patients with advanced esophageal carcinoma were randomly assigned to two groups.All patients in both groups were received chemotherapy with 5-Fu plus DDP firstly.The patients in control group(25) were given Huisheng oral liquid 10 ml /three times per day，while others in observation group(37) were received Huisheng oral liquid， and autologous CIK cells treatment.The curative effect and changes of T-cell subgroup percentage were evaluated.Results: The mean percentages of CD8 + T-cell in observation group were lower than in control group; while CD3 + ，CD4 + T-cell and the ratio of CD4 + /CD8 + were increased compared with control.No significant difference of total therapeutic effect， life quality score and weight was observed the two groups (P0.05 ) .Conclusions: Autologous CIK cells treatment plus Huisheng oral liquid in patients with advanced esophageal carcinoma could promote cellular immunity function，increase total therapeutic effect and life quality with no extra adverse reaction.It is a safe and effective assistance therapeutic tool.
Objective: To observe the efficacy and cellular immune function of autologous cytokine induced killer (CIK) cells treatment plus Huisheng oral liquid in patients with advanced esophageal carcinoma.Methods: A total of 62 patients with advanced esophageal carcinoma were randomly assigned to two groups.All patients in both groups were received chemotherapy with 5-Fu plus DDP firstly.The patients in control group(25) were given Huisheng oral liquid 10 ml /three times per day，while others in observation group(37) were received Huisheng oral liquid， and autologous CIK cells treatment.The curative effect and changes of T-cell subgroup percentage were evaluated.Results: The mean percentages of CD8 + T-cell in observation group were lower than in control group; while CD3 + ，CD4 + T-cell and the ratio of CD4 + /CD8 + were increased compared with control.No significant difference of total therapeutic effect， life quality score and weight was observed the two groups (P0.05 ) .Conclusions: Autologous CIK cells treatment plus Huisheng oral liquid in patients with advanced esophageal carcinoma could promote cellular immunity function，increase total therapeutic effect and life quality with no extra adverse reaction.It is a safe and effective assistance therapeutic tool.
2012, 36(9): 1064-1066.
Abstract:
Objective: To evaluate the clinical effect of hip prosthetic replacement in treating unstable intertrochanteric fractures in senile patients.Methods: Totally 49 senile patients with unstable intertroehanteric fraetures were treated by hip prosthetic replacement， and its outcome evaluated by Harris scoring system.Results: Successful operation was achieved in 49 patients whose hip joint function recovered to the pre-injury level.The follow-up period ranged from 6 to 36 months.According to Harris standard-the rate of excellent and good was 83.8.Conclusions: It is a proper treatment for senile patients with osteoporosis and unstable femoral intertrochanteric fracture.
Objective: To evaluate the clinical effect of hip prosthetic replacement in treating unstable intertrochanteric fractures in senile patients.Methods: Totally 49 senile patients with unstable intertroehanteric fraetures were treated by hip prosthetic replacement， and its outcome evaluated by Harris scoring system.Results: Successful operation was achieved in 49 patients whose hip joint function recovered to the pre-injury level.The follow-up period ranged from 6 to 36 months.According to Harris standard-the rate of excellent and good was 83.8.Conclusions: It is a proper treatment for senile patients with osteoporosis and unstable femoral intertrochanteric fracture.
2012, 36(9): 1067-1068,1071.
Abstract:
Objective: To explore the clinical effects of LEEP combined interferon on cervical intraepithelial neoplasia(CIN) with high-risk human papilloma virus(HPV) infection.Methods: One hundred and twenty-six cases with CIN were randomly divided into the observation group and control(63 cases each group) .Two groups were treaed with LEEP after Hybrid Capture II generation(HC-II) test， and the surgical specimens were detected by pathological examination.The operation time，wound healing time and the postoperative complications incidence rates were observed.The observation group were added interferon-，but not for the control group.The postive rates of HPV of two groups were compared in the 6 and 12 months after surgery.Results: The operative and wound healing time， postoperative complications rats of two groups were not significant different(P0.05) .The cure rates of the observation and control group were 98.4% and 88.9 % respectively，which were not statistical significant(P0.05) in 6 months after surgery.The HPVpositive rates of the observation and control group were 6.3% and 30.2%， and 3.2% and 23.8% in 6 and 12 months after surgery respectively，which were statistical significant (P0.01) .Conclusions: LEEP combined interferon treatment can inhibit the HPV infection and reducing the recurrence of CIN.
Objective: To explore the clinical effects of LEEP combined interferon on cervical intraepithelial neoplasia(CIN) with high-risk human papilloma virus(HPV) infection.Methods: One hundred and twenty-six cases with CIN were randomly divided into the observation group and control(63 cases each group) .Two groups were treaed with LEEP after Hybrid Capture II generation(HC-II) test， and the surgical specimens were detected by pathological examination.The operation time，wound healing time and the postoperative complications incidence rates were observed.The observation group were added interferon-，but not for the control group.The postive rates of HPV of two groups were compared in the 6 and 12 months after surgery.Results: The operative and wound healing time， postoperative complications rats of two groups were not significant different(P0.05) .The cure rates of the observation and control group were 98.4% and 88.9 % respectively，which were not statistical significant(P0.05) in 6 months after surgery.The HPVpositive rates of the observation and control group were 6.3% and 30.2%， and 3.2% and 23.8% in 6 and 12 months after surgery respectively，which were statistical significant (P0.01) .Conclusions: LEEP combined interferon treatment can inhibit the HPV infection and reducing the recurrence of CIN.
2012, 36(9): 1069-1071.
Abstract:
Objective: To compare the safety and liver damage of three different blocking hepatic blood flow ways in hepatectomy.Methods: Sixty patients with hepatectomy were divided into group A， group B and group C，which were operated by blocking all hepatic inflow，hemi-hepatic inflow and reserving unilateral hepatic artery(20 cases each group) ， respectively.The operation time，blood loss and postoperative liver function recovery of three groups were compared.Results: Blood loss of group B was more than in group A(P0.05) .There were no statistical differences in the time of operation and hepatic portal occlusion， complication(P0.05) .The levels of alanine aminotransferase(ALT) in group A were higher than that of group B and C at the postoperative day 7(P0.01) .The levels of total bilirubin(TB) in group A，group B and C at the postoperative day 1 and 7 was not statistically significant (P0.05 ) .Conclusions: Hepatectomy with hemi-hepatic artery reserved is a safe and less damage to liver.
Objective: To compare the safety and liver damage of three different blocking hepatic blood flow ways in hepatectomy.Methods: Sixty patients with hepatectomy were divided into group A， group B and group C，which were operated by blocking all hepatic inflow，hemi-hepatic inflow and reserving unilateral hepatic artery(20 cases each group) ， respectively.The operation time，blood loss and postoperative liver function recovery of three groups were compared.Results: Blood loss of group B was more than in group A(P0.05) .There were no statistical differences in the time of operation and hepatic portal occlusion， complication(P0.05) .The levels of alanine aminotransferase(ALT) in group A were higher than that of group B and C at the postoperative day 7(P0.01) .The levels of total bilirubin(TB) in group A，group B and C at the postoperative day 1 and 7 was not statistically significant (P0.05 ) .Conclusions: Hepatectomy with hemi-hepatic artery reserved is a safe and less damage to liver.
2012, 36(9): 1072-1073.
Abstract:
Objective: To explore the clinical value of xiaozhiling under ultrasonography guidance in treatment of splenic cyst.Methods: Under ultrasonography guidance， the fluid of splenic cyst was pumped，and 1 /2 cyst-liquid of xiaozhiling was injected into cyst and took out 5 min later， then 1 /8 cyst-liquid xiaozhiling was injected again.Three months later， the cysts were measured again.If there were no significant changes in the splenic cyst， the treatment would be repeated once.Results: Ten of 12 cases recovered， the length of splenic cyst of 2 cases narrowed 2 cm or so and no cases had adverse reaction and reocurred in following up 6 months.Conclusions: The treatment of splenic cyst with injection of xiaozhiling under ultrasonography guidance is simple， few pains for patients， low cost，high safety and cure rate，wide indication and can keep splenic function， so as to be first chosen to treat splenic cyst.
Objective: To explore the clinical value of xiaozhiling under ultrasonography guidance in treatment of splenic cyst.Methods: Under ultrasonography guidance， the fluid of splenic cyst was pumped，and 1 /2 cyst-liquid of xiaozhiling was injected into cyst and took out 5 min later， then 1 /8 cyst-liquid xiaozhiling was injected again.Three months later， the cysts were measured again.If there were no significant changes in the splenic cyst， the treatment would be repeated once.Results: Ten of 12 cases recovered， the length of splenic cyst of 2 cases narrowed 2 cm or so and no cases had adverse reaction and reocurred in following up 6 months.Conclusions: The treatment of splenic cyst with injection of xiaozhiling under ultrasonography guidance is simple， few pains for patients， low cost，high safety and cure rate，wide indication and can keep splenic function， so as to be first chosen to treat splenic cyst.
2012, 36(9): 1074-1076.
Abstract:
Objective: To observe the effect of mixed liquid infusion on pediatric burn shock stage.Methods: One hundred and seventy-two patients whose age were 7 years old，total burn surface area 10% were analyzed， and divided into traditional infusion group A(89 cases) and mixed liquor style infusion group B(83 cases) ， in accordance with the infusion mode.The therapentic effects were compared among the two groups.Results: There were 15 cases in the two groups who through shock stage and left or turned hospital，1 cases of infection death，the rest were cured.Seven cases in group A suffered from brain edema，while in group B nobody had such symptom and only 3 cases had febrile convulsion and recovered after symptomatic treatment.Conclusions: Preparation of reasonable mixed liquid，can simplify the infusion process，to prevent shock and heart，lungs，and brain complications.
Objective: To observe the effect of mixed liquid infusion on pediatric burn shock stage.Methods: One hundred and seventy-two patients whose age were 7 years old，total burn surface area 10% were analyzed， and divided into traditional infusion group A(89 cases) and mixed liquor style infusion group B(83 cases) ， in accordance with the infusion mode.The therapentic effects were compared among the two groups.Results: There were 15 cases in the two groups who through shock stage and left or turned hospital，1 cases of infection death，the rest were cured.Seven cases in group A suffered from brain edema，while in group B nobody had such symptom and only 3 cases had febrile convulsion and recovered after symptomatic treatment.Conclusions: Preparation of reasonable mixed liquid，can simplify the infusion process，to prevent shock and heart，lungs，and brain complications.
2012, 36(9): 1076-1077.
Abstract:
Objective: To explore the efficiency， safety and microtraumatity of neuroendoscopic therapy for symptomatic arachnoid cysts of middle cranial fossa.Methods: Twelve cases with symptomatic arachnoid cysts of middle cranial fossa were treated with partial stripping of arachnold membranes combined with fistulation by neuroendoscopy.Results: The symptoms of 11 cases except 1 case with oculomotor nerve paralysis were obviously improved.Conclusions: Treatment of symptomatic arachnoid cysts of middle cranial fossa with neuroendoscopy is safety， good effects and less complications，which is worthy of being widely applied in clinic.
Objective: To explore the efficiency， safety and microtraumatity of neuroendoscopic therapy for symptomatic arachnoid cysts of middle cranial fossa.Methods: Twelve cases with symptomatic arachnoid cysts of middle cranial fossa were treated with partial stripping of arachnold membranes combined with fistulation by neuroendoscopy.Results: The symptoms of 11 cases except 1 case with oculomotor nerve paralysis were obviously improved.Conclusions: Treatment of symptomatic arachnoid cysts of middle cranial fossa with neuroendoscopy is safety， good effects and less complications，which is worthy of being widely applied in clinic.
2012, 36(9): 1078-1079.
Abstract:
Objective: To evaluate the clinical effects of locking compression plate(LCP) internal fixation on treating complex femoral shaft comminuted fracture.Methods: The data of 18 cases with femoral shaft comminuted fracture treated with LCP internal fixation were retrospectively analysed， and the efficacy was evaluated.Results: All cases were followed up for 7 to 24 months and were the bone healing.The average healing time was (15.2 2.5) weeks.Conclusions: LCP internal fixation for treating comminuted fracture of femoral shaft is fixed concrete and fitting to the biomechanical principle， and which can reduce to the damage of femoral periosteal blood supply and the non-union rate of fracture.It is one of the effective way of treating comminuted femoral fracture.
Objective: To evaluate the clinical effects of locking compression plate(LCP) internal fixation on treating complex femoral shaft comminuted fracture.Methods: The data of 18 cases with femoral shaft comminuted fracture treated with LCP internal fixation were retrospectively analysed， and the efficacy was evaluated.Results: All cases were followed up for 7 to 24 months and were the bone healing.The average healing time was (15.2 2.5) weeks.Conclusions: LCP internal fixation for treating comminuted fracture of femoral shaft is fixed concrete and fitting to the biomechanical principle， and which can reduce to the damage of femoral periosteal blood supply and the non-union rate of fracture.It is one of the effective way of treating comminuted femoral fracture.
2012, 36(9): 1080-1081.
Abstract:
Objective: To explore the clinical effects of treatment varicocele with two kinds of high ligation via retroperitoneum.Methods: Twenty-four patients with varicocele were divided into group A and group B.Patients of group A were treated with the high ligation of spermatic vein and artery via retroperitoneum，patients of group B were treated with the high ligation of spermatic vein via retroperitoneum.The complications and quality of semen of two groups were analysed.Results: The effects of two high ligations were good， and the average operation time and hospital stays were 25 mins and 4 days respectively.Two cases of group B recured，and the orchiatrophy and long-term complications of two groups were not found during following up for 6 to 12 months.Sperm density，motility rate and vigor in both groups were significantly improved after surgery(P0.01) .Conclusions: Two ligation procedures have obvious good effects including short hospital stays and low cost，which can meet the demands of rural medical characteristics.The surgery of group A has innovation， simple and easy to be spreaded.
Objective: To explore the clinical effects of treatment varicocele with two kinds of high ligation via retroperitoneum.Methods: Twenty-four patients with varicocele were divided into group A and group B.Patients of group A were treated with the high ligation of spermatic vein and artery via retroperitoneum，patients of group B were treated with the high ligation of spermatic vein via retroperitoneum.The complications and quality of semen of two groups were analysed.Results: The effects of two high ligations were good， and the average operation time and hospital stays were 25 mins and 4 days respectively.Two cases of group B recured，and the orchiatrophy and long-term complications of two groups were not found during following up for 6 to 12 months.Sperm density，motility rate and vigor in both groups were significantly improved after surgery(P0.01) .Conclusions: Two ligation procedures have obvious good effects including short hospital stays and low cost，which can meet the demands of rural medical characteristics.The surgery of group A has innovation， simple and easy to be spreaded.
2012, 36(9): 1082-1084.
Abstract:
Objective: To study the effect and advantages of hysteroscope electrotomy in treatment of intrauterine occupying lesions.Methods: Sixtynine cases of intrauterine lesions including 41 cases of submucous myoma of the uterus(type 0 28 cases， type Ⅰ 11 cases and type Ⅱ 2 cases) and 28 cases of endometrial polyps underwent hysteroscope electrotomy.Results: Transcervical resection was performed successfully on 68 cases， and one case with type Ⅱ submucous myoma was converted to open surgery.The average operation time was(31.4 11.0) min，blood loss(20.7 13.9) ml and hospital stay(5.74 1.70) d.Uterine bleeding continued for(5.33 1.47) d.No syndrome of transurethal resection，uterine perforation or injury of the adjacent organs occurred.The satisfaction rate reached 98.5% in the 6 months' follow-up.Conclusions: Hysteroscope is safe and effective for treatment of intrauterine occupying lesions with the advantages of little trauma and hemorrhage， rapid recovery and short hospital stay.Proper selection of operation indications and full mastery of operation skills may achieve satisfactory results.
Objective: To study the effect and advantages of hysteroscope electrotomy in treatment of intrauterine occupying lesions.Methods: Sixtynine cases of intrauterine lesions including 41 cases of submucous myoma of the uterus(type 0 28 cases， type Ⅰ 11 cases and type Ⅱ 2 cases) and 28 cases of endometrial polyps underwent hysteroscope electrotomy.Results: Transcervical resection was performed successfully on 68 cases， and one case with type Ⅱ submucous myoma was converted to open surgery.The average operation time was(31.4 11.0) min，blood loss(20.7 13.9) ml and hospital stay(5.74 1.70) d.Uterine bleeding continued for(5.33 1.47) d.No syndrome of transurethal resection，uterine perforation or injury of the adjacent organs occurred.The satisfaction rate reached 98.5% in the 6 months' follow-up.Conclusions: Hysteroscope is safe and effective for treatment of intrauterine occupying lesions with the advantages of little trauma and hemorrhage， rapid recovery and short hospital stay.Proper selection of operation indications and full mastery of operation skills may achieve satisfactory results.
2012, 36(9): 1084-1085.
Abstract:
Objective: To explore the effects of termination time on perinatals with severe preeclampsia(PE) .Methods: The clinical data， complications and clinical monitoring index of 206 perinatals with evere PE were retrospectively analysed.Results: The incidences of complications of early-onset PE were 93.02%，which were higher than that of late-onset PE(83.33%) (P0.05) .The early-onset PE with Apgar score greater than 7 and weight larger than 1500g were lower than that of late-onset PE(P0.05 andP0.01) .The perinatal mortality rates of two types of were 18.60% and 3.33% respectively，which difference was statistically significant (P0.01) .Conclusions: Conservative treatment to extend gestational age for 32 weeks can improve perinatal outcome.Various monitoring methods are the premise of conservative treatment and the termination time can affect the perinatal outcome.
Objective: To explore the effects of termination time on perinatals with severe preeclampsia(PE) .Methods: The clinical data， complications and clinical monitoring index of 206 perinatals with evere PE were retrospectively analysed.Results: The incidences of complications of early-onset PE were 93.02%，which were higher than that of late-onset PE(83.33%) (P0.05) .The early-onset PE with Apgar score greater than 7 and weight larger than 1500g were lower than that of late-onset PE(P0.05 andP0.01) .The perinatal mortality rates of two types of were 18.60% and 3.33% respectively，which difference was statistically significant (P0.01) .Conclusions: Conservative treatment to extend gestational age for 32 weeks can improve perinatal outcome.Various monitoring methods are the premise of conservative treatment and the termination time can affect the perinatal outcome.
2012, 36(9): 1086-1087.
Abstract:
Objective: To investigate the clinical significance of the hemoglobin concentration(Hb) and the lymphocyte percentage.Methods: The Hb and lymphocyte percentage of 61 infants with recurrence thrush and 61 normal infants were measured wiht automated hematology analyzer.Results: The Hb and lymphocyte percentage of infants with recurrence thrush were lower and higher than that of normal infants respectively(P0.05 andP0.01) .Conclusions: The infants with recurrence thrush are related to the nutritional status and complicated infection.
Objective: To investigate the clinical significance of the hemoglobin concentration(Hb) and the lymphocyte percentage.Methods: The Hb and lymphocyte percentage of 61 infants with recurrence thrush and 61 normal infants were measured wiht automated hematology analyzer.Results: The Hb and lymphocyte percentage of infants with recurrence thrush were lower and higher than that of normal infants respectively(P0.05 andP0.01) .Conclusions: The infants with recurrence thrush are related to the nutritional status and complicated infection.
2012, 36(9): 1088-1090.
Abstract:
Objective: To summarize the methods and effects of early intervention on rehabilitation of children with brain injury.Methods: One hundred and twenty infants with high-risk brain injury were observed， 60 children were as treatment group， and other 60 children were as control group.The children in control group were given routine monitoring， the others in treatment group were given early intervention of additional measures.Results: After 1 years of following-up， the children in treatment group were better than in the control group at languages， sports， social ability， etc.The developmental quotient in the two groups was significant different(P0.01) .Conclusions: Early intervention is the good method in the treatment of children with brain injury， the sport and intellectual levels were raised，which can improve the prognosis.
Objective: To summarize the methods and effects of early intervention on rehabilitation of children with brain injury.Methods: One hundred and twenty infants with high-risk brain injury were observed， 60 children were as treatment group， and other 60 children were as control group.The children in control group were given routine monitoring， the others in treatment group were given early intervention of additional measures.Results: After 1 years of following-up， the children in treatment group were better than in the control group at languages， sports， social ability， etc.The developmental quotient in the two groups was significant different(P0.01) .Conclusions: Early intervention is the good method in the treatment of children with brain injury， the sport and intellectual levels were raised，which can improve the prognosis.
2012, 36(9): 1116-1118.
Abstract:
Objective: To explore the value of high-frequency color Doppler ultrasound in diagnosis of the benign and malignant of the neck superficial enlarged lymph nodes.Methods: Eighty-nine cases with the neck superficial enlarged lymph nodes were detected by high frequency ultrasound Doppler ultrasound.The sizes and ratio value of long diameter /short diameter ratio(L/S) ， shape，border， internal echo， resistance index(RI) and blood type and other sonographic features of the enlarged lymph nodes were observed.Results: The ratio value of L/S，boundary conditions， internal echo， lymph node door，blood type and RI of enlarged lymph nodes of the benign and malignant groups were statistically significant difference(P0.01) .Conclusions: The two-dimensional echocardiography combined with color Doppler ultrasound can diagnose the benign and malignant of neck superficial enlarged lymph nodes in a certain extent，which has important guiding significance in clinic.
Objective: To explore the value of high-frequency color Doppler ultrasound in diagnosis of the benign and malignant of the neck superficial enlarged lymph nodes.Methods: Eighty-nine cases with the neck superficial enlarged lymph nodes were detected by high frequency ultrasound Doppler ultrasound.The sizes and ratio value of long diameter /short diameter ratio(L/S) ， shape，border， internal echo， resistance index(RI) and blood type and other sonographic features of the enlarged lymph nodes were observed.Results: The ratio value of L/S，boundary conditions， internal echo， lymph node door，blood type and RI of enlarged lymph nodes of the benign and malignant groups were statistically significant difference(P0.01) .Conclusions: The two-dimensional echocardiography combined with color Doppler ultrasound can diagnose the benign and malignant of neck superficial enlarged lymph nodes in a certain extent，which has important guiding significance in clinic.
2012, 36(9): 1118-1120.
Abstract:
Objective: To evaluate the performance and features of lung laceration in the CT scan.Methods: The data of 11 cases of pulmonary lacerations of the CT scan were retrospectively analysised.Results: Eleven cases were involving 12 side with lung laceration， 18 disease lesions，most of which were on the back of the lung， close to the pleura and spine， single or multiple.CT detected 4 cases of lung cyst in，4 cases of cyst liquid，pulmonary hematoma in 3 cases.Eleven cases of lung laceration were all suffered varying degrees of pulmonary contusion， including 7 cases of rib fracture，6 cases of pleural effusion，6 cases of pneumothorax and 4 cases of subcutaneous emphysema.Conclusions: The CT manifestations of lung tear has a certain characteristic which could be accurate and timely diagnosis of the scope and severity of lung laceration on an important role in guiding clinical treatment.
Objective: To evaluate the performance and features of lung laceration in the CT scan.Methods: The data of 11 cases of pulmonary lacerations of the CT scan were retrospectively analysised.Results: Eleven cases were involving 12 side with lung laceration， 18 disease lesions，most of which were on the back of the lung， close to the pleura and spine， single or multiple.CT detected 4 cases of lung cyst in，4 cases of cyst liquid，pulmonary hematoma in 3 cases.Eleven cases of lung laceration were all suffered varying degrees of pulmonary contusion， including 7 cases of rib fracture，6 cases of pleural effusion，6 cases of pneumothorax and 4 cases of subcutaneous emphysema.Conclusions: The CT manifestations of lung tear has a certain characteristic which could be accurate and timely diagnosis of the scope and severity of lung laceration on an important role in guiding clinical treatment.
2012, 36(9): 1121-1122.
Abstract:
Objective: To investigate the relationship of prognosis and the numbers of white blood cell(WBC) and neutrophil(N) in early stage of cerebral hemorrhage.Methods: The total numbers of white blood cell and N in early stage of cerebral hemorrhage were observed，which were divided into divided into normal group(10 109 /L) ，high group［(10- 14) 109 /L］ and significantly higher group( 14 109 /L) according to the total counts of WBC， and the same three groups were divided accoridng to the N0.75，0 .76 N 0.90 and N 0.90.Results: Compared with the normal group，heavy patients of high and significantly higher groups of WBC classification increased，which fatality rates was statistical significant(P0.01) .The heavy patients of high and significantly higher groups of N classification were higher than that of the normal group (P0.05) ，and the survival rates of the normal group were significantly higher than that of significantly higher group(P0.05) .Conclusions: The increased numbers of WBC and N of patients with acute cerebral hemorrhage in the early stage suggest a serious condition，poor efficacy and high mortality，which can be used as a prognostic clinical index.
Objective: To investigate the relationship of prognosis and the numbers of white blood cell(WBC) and neutrophil(N) in early stage of cerebral hemorrhage.Methods: The total numbers of white blood cell and N in early stage of cerebral hemorrhage were observed，which were divided into divided into normal group(10 109 /L) ，high group［(10- 14) 109 /L］ and significantly higher group( 14 109 /L) according to the total counts of WBC， and the same three groups were divided accoridng to the N0.75，0 .76 N 0.90 and N 0.90.Results: Compared with the normal group，heavy patients of high and significantly higher groups of WBC classification increased，which fatality rates was statistical significant(P0.01) .The heavy patients of high and significantly higher groups of N classification were higher than that of the normal group (P0.05) ，and the survival rates of the normal group were significantly higher than that of significantly higher group(P0.05) .Conclusions: The increased numbers of WBC and N of patients with acute cerebral hemorrhage in the early stage suggest a serious condition，poor efficacy and high mortality，which can be used as a prognostic clinical index.
2012, 36(9): 1125-1126.
Abstract:
Objective: To analyze the effect of standardized process on the cleaning and packaging quality of operational instruments for outpatients.Methods: The cleaning quality and functional integrity of the operational devices， and the quality index referring to the weight and cubic measure of the package， the instruction cards within the package as well as the tags outside were compared before and after the application of standardized management.Results: The overall qualification rates of the cleaning and packaging quality of the instruments before and after the application of standardized process were 99.4% and 87.5%， respectively(P0.01) .Conclusions: Standardized management can effectively improve the cleaning and packaging quality of operational instruments for outpatients，which are beneficial to the succeeding disinfection and sterilization.
Objective: To analyze the effect of standardized process on the cleaning and packaging quality of operational instruments for outpatients.Methods: The cleaning quality and functional integrity of the operational devices， and the quality index referring to the weight and cubic measure of the package， the instruction cards within the package as well as the tags outside were compared before and after the application of standardized management.Results: The overall qualification rates of the cleaning and packaging quality of the instruments before and after the application of standardized process were 99.4% and 87.5%， respectively(P0.01) .Conclusions: Standardized management can effectively improve the cleaning and packaging quality of operational instruments for outpatients，which are beneficial to the succeeding disinfection and sterilization.
2012, 36(9): 1127-1129.
Abstract:
Objective: To summarize the nursing experience in treatment of simple great saphenous vein (GSV) varicosis with endovenous laser combined with high ligation.Methods: Twenty-three cases with GSV varicosis treated with endovenous laser combined with high ligation were nursed and educated on healfthy in preoperative and postoperative.Results: All cases cured without severe complications such as lower limb deep vein thrombosis.Conclusions: Treatment of simple GSV varicosis with endovenous laser combined with high ligation is simple to operate，quick recovery， small scar， short hospitalizing time and less of recur.Postoperative nursing is a central part of the successful operation.
Objective: To summarize the nursing experience in treatment of simple great saphenous vein (GSV) varicosis with endovenous laser combined with high ligation.Methods: Twenty-three cases with GSV varicosis treated with endovenous laser combined with high ligation were nursed and educated on healfthy in preoperative and postoperative.Results: All cases cured without severe complications such as lower limb deep vein thrombosis.Conclusions: Treatment of simple GSV varicosis with endovenous laser combined with high ligation is simple to operate，quick recovery， small scar， short hospitalizing time and less of recur.Postoperative nursing is a central part of the successful operation.
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