Author Login
Experts Review
Academic Editor
Editor-in-chief
2013 Vol. 38, No. 10
Display Method:
2013, 37(10): 1229-1234.
Abstract:
Objective: To observe the effect of probucol(PB) on the expression of transforming growth factor-1 and hemeoxygenase-1 (HO-1) in the renal tissue of diabetic nephropathy (DN) rats，and investigate the mechanism of PB in delaying renal interstitial fibrosis. Methods: Fifty male Sprague-Dawlay(SD) rats were randomly divided into experimental group and control group. The rats in the experimental group were given high-sugar，high-fat diet and intraperitoneal injection of streptozotocin to establish DN rat models, the model rats were then randomly divided into DN group and PB group. The rats in the PB group were treated by oral gavage of PB 500 mgkg-1d-1 . At the end of the experiment, the rats were weighed and the 24 h urinary protein and urinary N-acetyl--Dglucosaminidase(NAG) were checked, the rats were killed to collect the blood; and the serum biochemical parameters were observed, the kidney of the model rats was removed and performed tissue section to observe the renal interstitial fibrosis, the expression of renal tissue HO-1 and TGF-1 was detected by immunohistochemistry，the nucleic acid expression of the renal tissue TGF-1 and HO-1 was detected by rT-PCr. results: PB could relieve the proteinuria in the rats with DN，degrade the urinary NAG level, improve the serum total cholesterol, and reduce the serum creatinine and blood urea nitrogen level. It could also upgrade the level of HO-1mrNA kidney tissue and downgrade the level of TGF-1mrNA. Conclusions: PB can delay renal fibrosis, the mechanism of which may be that PB could up-regulate the expression of HO-1 and down-regulate the expression of TGF-1 .
Objective: To observe the effect of probucol(PB) on the expression of transforming growth factor-1 and hemeoxygenase-1 (HO-1) in the renal tissue of diabetic nephropathy (DN) rats，and investigate the mechanism of PB in delaying renal interstitial fibrosis. Methods: Fifty male Sprague-Dawlay(SD) rats were randomly divided into experimental group and control group. The rats in the experimental group were given high-sugar，high-fat diet and intraperitoneal injection of streptozotocin to establish DN rat models, the model rats were then randomly divided into DN group and PB group. The rats in the PB group were treated by oral gavage of PB 500 mgkg-1d-1 . At the end of the experiment, the rats were weighed and the 24 h urinary protein and urinary N-acetyl--Dglucosaminidase(NAG) were checked, the rats were killed to collect the blood; and the serum biochemical parameters were observed, the kidney of the model rats was removed and performed tissue section to observe the renal interstitial fibrosis, the expression of renal tissue HO-1 and TGF-1 was detected by immunohistochemistry，the nucleic acid expression of the renal tissue TGF-1 and HO-1 was detected by rT-PCr. results: PB could relieve the proteinuria in the rats with DN，degrade the urinary NAG level, improve the serum total cholesterol, and reduce the serum creatinine and blood urea nitrogen level. It could also upgrade the level of HO-1mrNA kidney tissue and downgrade the level of TGF-1mrNA. Conclusions: PB can delay renal fibrosis, the mechanism of which may be that PB could up-regulate the expression of HO-1 and down-regulate the expression of TGF-1 .
2013, 37(10): 1234-1237.
Abstract:
Objective: To investigate the expressions of Twist protein (Twist) ，signal transducer and activator of transcription 3 (STAT3) ，epithelial mesenchymal transition related factor (E-cad) in gastric cancer tissue and peritumoral tissue，analyze their correlations and explore their significance in judging the prognosis of patients with gastric cancer. Methods: The expressions of Twist, STAT3 and E-cad in gastric cancer tissue and peritumoral tissue were detected by immunohistochemistry streptavidin-biotin complex (SABC) , their relationships with age, gender，histological type of tumor，metastasis and TNM stage of patient were analysed. results: The positive rate of Twist，STAT3 and E-cad in gastric cancer tissue were 57. 3%, 64. 7% and 12. 0%, respectively. The positive rate of Twist，STAT3 and E-cad in peritumoral tissue were 17. 3%, 10. 8% and 68. 0%, the expression differences of which were not statistical significance(P ＜ 0. 01) . The differences of Twist expression in different tumor metastasis, lymph node metastasis，TNM staging and depth of invasion were statistical significance(P ＜ 0. 05-P ＜ 0. 01) . There was statistically significant in the expression of STAT3 between node metastasis，depth of invasion and TNM stage(P ＜ 0. 05-P ＜ 0. 01) . They were negative correlation in Twist and STAT3 expressions and E-cad expression(P ＜ 0. 01) . Conclusions: The expressions of Twist and SATA in gastric cancer tissue have a certain relationship with tumor biological characteristics, the expression of SATA3 may activate the expression of Twist and E-cad. The detection of Twist，STAT3 and E-cad can predict the tumor invasion，metastasis and prognosis of patient.
Objective: To investigate the expressions of Twist protein (Twist) ，signal transducer and activator of transcription 3 (STAT3) ，epithelial mesenchymal transition related factor (E-cad) in gastric cancer tissue and peritumoral tissue，analyze their correlations and explore their significance in judging the prognosis of patients with gastric cancer. Methods: The expressions of Twist, STAT3 and E-cad in gastric cancer tissue and peritumoral tissue were detected by immunohistochemistry streptavidin-biotin complex (SABC) , their relationships with age, gender，histological type of tumor，metastasis and TNM stage of patient were analysed. results: The positive rate of Twist，STAT3 and E-cad in gastric cancer tissue were 57. 3%, 64. 7% and 12. 0%, respectively. The positive rate of Twist，STAT3 and E-cad in peritumoral tissue were 17. 3%, 10. 8% and 68. 0%, the expression differences of which were not statistical significance(P ＜ 0. 01) . The differences of Twist expression in different tumor metastasis, lymph node metastasis，TNM staging and depth of invasion were statistical significance(P ＜ 0. 05-P ＜ 0. 01) . There was statistically significant in the expression of STAT3 between node metastasis，depth of invasion and TNM stage(P ＜ 0. 05-P ＜ 0. 01) . They were negative correlation in Twist and STAT3 expressions and E-cad expression(P ＜ 0. 01) . Conclusions: The expressions of Twist and SATA in gastric cancer tissue have a certain relationship with tumor biological characteristics, the expression of SATA3 may activate the expression of Twist and E-cad. The detection of Twist，STAT3 and E-cad can predict the tumor invasion，metastasis and prognosis of patient.
2013, 37(10): 1238-1240.
Abstract:
Objective: To study the biodistribution of 99Tcm-luteinzing hormone-releasing hormone (99 Tcm-LHrH) in nude mice bearing human breast cancer, and to evaluate the effect of cancer diagnosis and targeted radiotherapy with 99Tcm-LHrH in nude mice bearing human breast cancer. Methods: LHrH was directly radio labeled with 99Tcm . Twenty-five nude mice were randomly divided into five groups, five in each. Nude mice bearing SK-br3 breast cancer cells were injected with 99Tcm-LHrH by vein caudalis，and sacrificed. Detect the biodistribution of 99Tcm-LHrH and calculated per gram Organization percentage injected dose rate. results: The result showed that radiochemical purity of 99Tcm-LHrH was more than 95%. The ratios of tumor to blood and tumor to muscle were 12. 89 1. 67, and 36. 81 5. 64, respectively. The biodistribution experiment showed that 99Tcm-LHrH uptake by liver，blood, tumor site was higher than by muscle and brain. Conclusions: 99Tcm-LHrH has good specificity to human breast cancer cell. It may be used in the targeted diagnosis and therapy of breast cancer.
Objective: To study the biodistribution of 99Tcm-luteinzing hormone-releasing hormone (99 Tcm-LHrH) in nude mice bearing human breast cancer, and to evaluate the effect of cancer diagnosis and targeted radiotherapy with 99Tcm-LHrH in nude mice bearing human breast cancer. Methods: LHrH was directly radio labeled with 99Tcm . Twenty-five nude mice were randomly divided into five groups, five in each. Nude mice bearing SK-br3 breast cancer cells were injected with 99Tcm-LHrH by vein caudalis，and sacrificed. Detect the biodistribution of 99Tcm-LHrH and calculated per gram Organization percentage injected dose rate. results: The result showed that radiochemical purity of 99Tcm-LHrH was more than 95%. The ratios of tumor to blood and tumor to muscle were 12. 89 1. 67, and 36. 81 5. 64, respectively. The biodistribution experiment showed that 99Tcm-LHrH uptake by liver，blood, tumor site was higher than by muscle and brain. Conclusions: 99Tcm-LHrH has good specificity to human breast cancer cell. It may be used in the targeted diagnosis and therapy of breast cancer.
2013, 37(10): 1241-1245.
Abstract:
Objective: To investigate the effect and mechanism of hydrogen-rich saline on rat intestine damage after severe burn. Methods: Forty eight rats were divided into four groups: sham plus normal saline(S) group，burn injury plus normal saline(B + N) group，burn injury plus hydrogen-rich saline(B + H) group, and burn injury plus vitamin C(B + C) group. Each group contains 12 rats. Animals were subjected to full-thickness burn wound(30% total body surface area) using boiling water, except the rats in sham group which were treated with room-temperature water. Lactated ringer's solution was given at 6 h after injury according to Parkland formula. The rats in B + H group were received 5 ml /kg of hydrogen-rich saline, others in S and B + N groups were received the same amount of saline, and the rats in B + C group were treated with 9 ml /kg(250 mg /kg) of vitamin C in saline at 6，18，30，42 hour after scald respectively. The intestinal pathological changes at 24 and 48 h after scald was investigated. The changes of malondialdehyde，diamine oxidase, interleukin-1 and apoptosis rate of intestinal epithelial cells were determined. results: Compared with B + N group, in B + C and B + H groups, the intestinal pathological changes were significantly reduced，diamine oxidase activity was increased significantly(P ＜ 0. 01) ，and malondialdehyde, interleukin-1 levels and apoptosis rate of intestinal epithelial cells were significantly reduced(P ＜ 0. 01) . Conclusions: Hydrogen-rich saline can protect the small intestine against ischemia and reperfusion injury after severe burn, its mechanisms include relief of oxidative stress, inflammatory factor-mediated inflammatory response and inhibition of intestinal epithelial cell apoptosis.
Objective: To investigate the effect and mechanism of hydrogen-rich saline on rat intestine damage after severe burn. Methods: Forty eight rats were divided into four groups: sham plus normal saline(S) group，burn injury plus normal saline(B + N) group，burn injury plus hydrogen-rich saline(B + H) group, and burn injury plus vitamin C(B + C) group. Each group contains 12 rats. Animals were subjected to full-thickness burn wound(30% total body surface area) using boiling water, except the rats in sham group which were treated with room-temperature water. Lactated ringer's solution was given at 6 h after injury according to Parkland formula. The rats in B + H group were received 5 ml /kg of hydrogen-rich saline, others in S and B + N groups were received the same amount of saline, and the rats in B + C group were treated with 9 ml /kg(250 mg /kg) of vitamin C in saline at 6，18，30，42 hour after scald respectively. The intestinal pathological changes at 24 and 48 h after scald was investigated. The changes of malondialdehyde，diamine oxidase, interleukin-1 and apoptosis rate of intestinal epithelial cells were determined. results: Compared with B + N group, in B + C and B + H groups, the intestinal pathological changes were significantly reduced，diamine oxidase activity was increased significantly(P ＜ 0. 01) ，and malondialdehyde, interleukin-1 levels and apoptosis rate of intestinal epithelial cells were significantly reduced(P ＜ 0. 01) . Conclusions: Hydrogen-rich saline can protect the small intestine against ischemia and reperfusion injury after severe burn, its mechanisms include relief of oxidative stress, inflammatory factor-mediated inflammatory response and inhibition of intestinal epithelial cell apoptosis.
2013, 37(10): 1246-1248.
Abstract:
Objective: To explore the level of nitric oxide(NO) in osteoblasts treated with different concentrations of tumor necrosis factor-(TNF-) . Methods: The osteoblasts were cultured and identified. The NO level in cultured supernafants in osteoblasts treated with different concentrations of TNF- was measured. results: The level of the NO was increased with the increase of concentration of TNF-，which was the maximum at 10 g /L of TNF-. The level of the NO began to decrease with the increase of processing time and concentration of TNF-(P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: TNF- can induce the expression of NO，but TNF- can not countine to increase the expression of NO with the increase of its processing time and concentration in cultured osteoblasts.
Objective: To explore the level of nitric oxide(NO) in osteoblasts treated with different concentrations of tumor necrosis factor-(TNF-) . Methods: The osteoblasts were cultured and identified. The NO level in cultured supernafants in osteoblasts treated with different concentrations of TNF- was measured. results: The level of the NO was increased with the increase of concentration of TNF-，which was the maximum at 10 g /L of TNF-. The level of the NO began to decrease with the increase of processing time and concentration of TNF-(P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: TNF- can induce the expression of NO，but TNF- can not countine to increase the expression of NO with the increase of its processing time and concentration in cultured osteoblasts.
2013, 37(10): 1249-1252.
Abstract:
Objective: To study the effect of rapamycin alone or combined with cisplatin on apoptosis and cell cycle distribution of human ovarian cancer SKOV3 cells, and elucidate its possible mechanism. Methods: SKOV3 cells were cultured in vitro, the effect of rapamycin alone or combined with cisplatin on apoptosis and cell cycle distribution were examined by flow cytometry assay. The expression of mammalian target of rapamycin (mTOr) mrNA was detected by qrT-PCr. results: rapamycin alone inhibited the growth of SKOV3 cells, and caused cell cycle arresting at G1 phase，decreased cell number at S and G2 phases, then induced apoptosis happening，which reached the highest inhibition rate at 72 h, and the apoptosis of rapamycin combined with cisplatin was higher than in rapamycin alone group，which had statistical significance(P ＜ 0. 01) . The qrT-PCr results showed that the expression of mTOr mrNA was decreased in SKOV3 cells treated with rapamycin after 24 h, and further decreased at 72 h(P ＜ 0. 05) . Conclusions: rapamycin inhibits mTOr expression in PI3K/AKT/mTOr cell signal pathway, caused the cell cycle arresting at G1 phase and induced apoptosis. The two drugs in combination show synergy effect, and rapamycin can strengthen the tumor cells chemotherapy sensitivity to cisplatin.
Objective: To study the effect of rapamycin alone or combined with cisplatin on apoptosis and cell cycle distribution of human ovarian cancer SKOV3 cells, and elucidate its possible mechanism. Methods: SKOV3 cells were cultured in vitro, the effect of rapamycin alone or combined with cisplatin on apoptosis and cell cycle distribution were examined by flow cytometry assay. The expression of mammalian target of rapamycin (mTOr) mrNA was detected by qrT-PCr. results: rapamycin alone inhibited the growth of SKOV3 cells, and caused cell cycle arresting at G1 phase，decreased cell number at S and G2 phases, then induced apoptosis happening，which reached the highest inhibition rate at 72 h, and the apoptosis of rapamycin combined with cisplatin was higher than in rapamycin alone group，which had statistical significance(P ＜ 0. 01) . The qrT-PCr results showed that the expression of mTOr mrNA was decreased in SKOV3 cells treated with rapamycin after 24 h, and further decreased at 72 h(P ＜ 0. 05) . Conclusions: rapamycin inhibits mTOr expression in PI3K/AKT/mTOr cell signal pathway, caused the cell cycle arresting at G1 phase and induced apoptosis. The two drugs in combination show synergy effect, and rapamycin can strengthen the tumor cells chemotherapy sensitivity to cisplatin.
2013, 37(10): 1253-1256,1259.
Abstract:
Objective: To investigate the clinical manifestation and the levels of interleukin-2 (IL-2) , interleukin-12 (IL-12) and monocyte chemotactic protein-1 (MCP-1 ) of SD rats with pneumonia induced by Klebsiella pneumonia and their changes after intervention. Methods: Four to six weeks old SD rats were randomly divided into the model group, control group and intervention group (8 rats each group) . The model and intervention group were inoculated with the same concentration and dose of Klebsiella pneumoniae bacteria, and the control group were inoculated with normal saline. The intervention group were treated with cefoperazone sodium and sulbactam sodium at 24 hours after inoculation. Each group were detected by X-ray through random sample method. The levels of IL-2, IL-12 and MCP-1 of all groups were measured at d3 ，d5 and d7 . results: The levels of IL-2, IL-12 and MCP-1 in model group increased at d3. Compared with the control group, there was significant difference(P ＜ 0. 01) . Compared with intervention group, there was no significant difference(P ＞ 0. 05) . The levels of IL-2, IL-12 and MCP-1 in model group continued to increase at d5 . Compared with the control and intervention group, their differences were statistically significant(P ＜ 0. 01 and P ＜ 0. 05) . The levels of IL-2, IL-12 and MCP-1 in intervention group droped to normal. The levels of IL-2, IL-12 and MCP-1 in model group continued to increase at d7 . Compared with the control and intervention group, their differences had statistical significance(P ＜ 0. 01) . Conclusions: The serum levels of IL-2，IL-12 and MCP-1 increase gradually in the acute infection period of rats with pneumonia induced by Klebsiella pneumonia，which indicates that these factors involve the pathophysiological process of Klebsiella pneumoniae. Dynamic monitoring the serum levels of IL-2, IL-12 and MCP-1 can judge the illness and prognosis of pneumonia induced by Klebsiella pneumonia.
Objective: To investigate the clinical manifestation and the levels of interleukin-2 (IL-2) , interleukin-12 (IL-12) and monocyte chemotactic protein-1 (MCP-1 ) of SD rats with pneumonia induced by Klebsiella pneumonia and their changes after intervention. Methods: Four to six weeks old SD rats were randomly divided into the model group, control group and intervention group (8 rats each group) . The model and intervention group were inoculated with the same concentration and dose of Klebsiella pneumoniae bacteria, and the control group were inoculated with normal saline. The intervention group were treated with cefoperazone sodium and sulbactam sodium at 24 hours after inoculation. Each group were detected by X-ray through random sample method. The levels of IL-2, IL-12 and MCP-1 of all groups were measured at d3 ，d5 and d7 . results: The levels of IL-2, IL-12 and MCP-1 in model group increased at d3. Compared with the control group, there was significant difference(P ＜ 0. 01) . Compared with intervention group, there was no significant difference(P ＞ 0. 05) . The levels of IL-2, IL-12 and MCP-1 in model group continued to increase at d5 . Compared with the control and intervention group, their differences were statistically significant(P ＜ 0. 01 and P ＜ 0. 05) . The levels of IL-2, IL-12 and MCP-1 in intervention group droped to normal. The levels of IL-2, IL-12 and MCP-1 in model group continued to increase at d7 . Compared with the control and intervention group, their differences had statistical significance(P ＜ 0. 01) . Conclusions: The serum levels of IL-2，IL-12 and MCP-1 increase gradually in the acute infection period of rats with pneumonia induced by Klebsiella pneumonia，which indicates that these factors involve the pathophysiological process of Klebsiella pneumoniae. Dynamic monitoring the serum levels of IL-2, IL-12 and MCP-1 can judge the illness and prognosis of pneumonia induced by Klebsiella pneumonia.
2013, 37(10): 1257-1259.
Abstract:
Objective: To explore the clinical characteristics，diagnosis and treatment measures of abdominal compartment syndrome (ACS) . Methods: Thirty-eight patients with ACS were divided into surgical treatment group (30 cases) and conservative treatment group(8 cases) according to the patient's condition. The complications，death，cured, intra-abdominal pressure and clinical symptoms were compared. results: The total incidence rate of complication, fatality rate and cure rate were 42. 1%，31. 6% and 68. 4%, respectively. Complications in 3 cases and 13 cases were found in conservative treatment group and surgical treatment group, respectively. Death in 3 cases and 9 cases were found in conservative treatment group and surgical treatment group, respectively. Cure in 5 cases and 21 cases were found in conservative treatment group and surgical treatment group, respectively, the differeces of which were not statistically significant(P = 1. 000) . ACS signs were found in 38 cases, intra-abdominal pressure(more than 20 mmHg) elevated stability by cystometry, accompanied with shock, acute respiratory distress syndrome and multiple organ dysfunction syndrome in more cases. Two or more organ dysfunction in 26 cases were found. Conclusions: Mastering appropriate treatment timing, indications and method are vital to the prognosis of ACS.
Objective: To explore the clinical characteristics，diagnosis and treatment measures of abdominal compartment syndrome (ACS) . Methods: Thirty-eight patients with ACS were divided into surgical treatment group (30 cases) and conservative treatment group(8 cases) according to the patient's condition. The complications，death，cured, intra-abdominal pressure and clinical symptoms were compared. results: The total incidence rate of complication, fatality rate and cure rate were 42. 1%，31. 6% and 68. 4%, respectively. Complications in 3 cases and 13 cases were found in conservative treatment group and surgical treatment group, respectively. Death in 3 cases and 9 cases were found in conservative treatment group and surgical treatment group, respectively. Cure in 5 cases and 21 cases were found in conservative treatment group and surgical treatment group, respectively, the differeces of which were not statistically significant(P = 1. 000) . ACS signs were found in 38 cases, intra-abdominal pressure(more than 20 mmHg) elevated stability by cystometry, accompanied with shock, acute respiratory distress syndrome and multiple organ dysfunction syndrome in more cases. Two or more organ dysfunction in 26 cases were found. Conclusions: Mastering appropriate treatment timing, indications and method are vital to the prognosis of ACS.
2013, 37(10): 1260-1263.
Abstract:
Objective: To explore the expression of signal transducer and activator of transcription 3(STAT3) in osteosarcoma and its relationship with tumor microvascular density(MVD) . Methods: The STAT3 expression of 47 cases with osteosarcoma were detected by immunohistochemistry. The clinical data，pathological classification and clinical index of 47 cases were compared. MVD of osteosarcoma was counted. The correlation between the expression of STAT3 and MVD was analyzed. results: The exprssion of STAT3 was mainly in the cytoplasm. The expression levels of STAT3 in osteosarcoma were significantly higher than that in normal bone tissue(P ＜ 0. 01) . The expression levels of STAT3 in osteosarcoma with stage Ⅱa，Ⅱb and Ⅲ were significantly higher than those in stage Ⅰ(P ＜ 0. 01) , the expression of stage Ⅲ was significantly higher than stage Ⅱa and Ⅱb(P ＜ 0. 01) . The expression levels of STAT3 in invasive soft tissue were significantly higher than that in no-invasive soft tissue(P ＜ 0. 01) . The expression of STAT3 in osteosarcoma was positively correlated with MVD(P ＜ 0. 05) . Conclusions: The expression of STAT3 in osteosarcoma is correlated with tumor clinical stage and soft tissue invasion, and its expression is positively correlated with MVD. The high expression of STAT3 may play an important role in the development of osteosarcoma, and which is closely correlated to the angiogenesis of osteosarcoma.
Objective: To explore the expression of signal transducer and activator of transcription 3(STAT3) in osteosarcoma and its relationship with tumor microvascular density(MVD) . Methods: The STAT3 expression of 47 cases with osteosarcoma were detected by immunohistochemistry. The clinical data，pathological classification and clinical index of 47 cases were compared. MVD of osteosarcoma was counted. The correlation between the expression of STAT3 and MVD was analyzed. results: The exprssion of STAT3 was mainly in the cytoplasm. The expression levels of STAT3 in osteosarcoma were significantly higher than that in normal bone tissue(P ＜ 0. 01) . The expression levels of STAT3 in osteosarcoma with stage Ⅱa，Ⅱb and Ⅲ were significantly higher than those in stage Ⅰ(P ＜ 0. 01) , the expression of stage Ⅲ was significantly higher than stage Ⅱa and Ⅱb(P ＜ 0. 01) . The expression levels of STAT3 in invasive soft tissue were significantly higher than that in no-invasive soft tissue(P ＜ 0. 01) . The expression of STAT3 in osteosarcoma was positively correlated with MVD(P ＜ 0. 05) . Conclusions: The expression of STAT3 in osteosarcoma is correlated with tumor clinical stage and soft tissue invasion, and its expression is positively correlated with MVD. The high expression of STAT3 may play an important role in the development of osteosarcoma, and which is closely correlated to the angiogenesis of osteosarcoma.
2013, 37(10): 1263-1265.
Abstract:
Objective: To explore the efficacy of systemic chemotherapy combined with percutaneous ethanol injection(PEI) treatment on breast carcinoma postoperative patients with liver metastasis. Methods: Fifteen cases were all breast cancer postoperative patients with liver metastasis. All patients were received paclitaxel toisplatin(TP) protocol. The treatment included systemic chemotherapy of paclitaxel 135 mg /m2 intravenous injection on first day followed by cisplatin 30 mg /d for 3 days, 28 days for one cycle, and each patient was experienced for more than 4 courses. Metastatic tumor foci of liver was also received therapy of PEI under ultrasound-guided. Each foci was experienced treatment for 2 to 4 times. results: Three cases were complete remission, 12 cases were partial remission, the total response rate was 100%. All patients survived for more than 6 months, in 10 cases of death, one patient survived for 22 months. Adverse reaction included lower appetite，nausea，vomiting，neutropenia, transient liver function abnormalities and the puncture site pain. Conclusions: The combination of PET and systemic chemotherapy by TP protocol may be an effective treatment to postoperative patients with breast carcinoma and hepatic metastasis.
Objective: To explore the efficacy of systemic chemotherapy combined with percutaneous ethanol injection(PEI) treatment on breast carcinoma postoperative patients with liver metastasis. Methods: Fifteen cases were all breast cancer postoperative patients with liver metastasis. All patients were received paclitaxel toisplatin(TP) protocol. The treatment included systemic chemotherapy of paclitaxel 135 mg /m2 intravenous injection on first day followed by cisplatin 30 mg /d for 3 days, 28 days for one cycle, and each patient was experienced for more than 4 courses. Metastatic tumor foci of liver was also received therapy of PEI under ultrasound-guided. Each foci was experienced treatment for 2 to 4 times. results: Three cases were complete remission, 12 cases were partial remission, the total response rate was 100%. All patients survived for more than 6 months, in 10 cases of death, one patient survived for 22 months. Adverse reaction included lower appetite，nausea，vomiting，neutropenia, transient liver function abnormalities and the puncture site pain. Conclusions: The combination of PET and systemic chemotherapy by TP protocol may be an effective treatment to postoperative patients with breast carcinoma and hepatic metastasis.
2013, 37(10): 1266-1267.
Abstract:
Objective: To explore the prevention and treatment of neck occipital effusion after posterior fossa operation. Methods: The clinical data of 15 cases with neck occipital effusion after posterior fossa operation were retrospectively analyzed. The drainage tube was indwelled in neck occipital effusion cavity of 15 patients. Effusion in 5 cases disappeared. External drainage of lumbar cistern were used in 10 cases with invalid drainage tube，effusion disappearing in 5 cases were found and 4 cases were treated with sutura primosecundaria. results: After treatment，no mild fever，headache symptoms and neck occipital effusion were found in 15 patients. Conclusions: Careful operation is the key to reduce effusion. A variety of treatment methods must be implemented for effusion patients, which can obtain good effects.
Objective: To explore the prevention and treatment of neck occipital effusion after posterior fossa operation. Methods: The clinical data of 15 cases with neck occipital effusion after posterior fossa operation were retrospectively analyzed. The drainage tube was indwelled in neck occipital effusion cavity of 15 patients. Effusion in 5 cases disappeared. External drainage of lumbar cistern were used in 10 cases with invalid drainage tube，effusion disappearing in 5 cases were found and 4 cases were treated with sutura primosecundaria. results: After treatment，no mild fever，headache symptoms and neck occipital effusion were found in 15 patients. Conclusions: Careful operation is the key to reduce effusion. A variety of treatment methods must be implemented for effusion patients, which can obtain good effects.
2013, 37(10): 1268-1269.
Abstract:
Objective: To explore the effects of Danhong injection on the levels of D-dimer(DD) in patients with chronic heart failure (CHF) . Methods: Thirty-eight patients with CHF complicated with high DD level were treated with 40 ml of Danhong injection for 10 to 14 days, the DD levels of all patients were detected. results: The levels of DD in 38 cases after treatment(0. 38 0. 21 g /ml) were significantly lower than that before treatment(1. 82 0. 20 g /ml) (P ＜ 0. 01) . The levels of DD in 7 cases at 7 days after treatment did not arrive at normal，which arrived at normal at 5 days after treatment with low molecular heparin. Conclusions: Danhong injection can significantly reduce the levels of DD in patients with CHF.
Objective: To explore the effects of Danhong injection on the levels of D-dimer(DD) in patients with chronic heart failure (CHF) . Methods: Thirty-eight patients with CHF complicated with high DD level were treated with 40 ml of Danhong injection for 10 to 14 days, the DD levels of all patients were detected. results: The levels of DD in 38 cases after treatment(0. 38 0. 21 g /ml) were significantly lower than that before treatment(1. 82 0. 20 g /ml) (P ＜ 0. 01) . The levels of DD in 7 cases at 7 days after treatment did not arrive at normal，which arrived at normal at 5 days after treatment with low molecular heparin. Conclusions: Danhong injection can significantly reduce the levels of DD in patients with CHF.
2013, 37(10): 1270-1271.
Abstract:
Objective: To explore the clinical effect of Nano-silver dressing in treatment of pediatric deep partial-thickness burn. Methods: A total of 80 patients with deep partial thickness burn were randomly divided into two groups，with 40 in each one. The control group was applied with silver zinc cream and the observation group was applied with nano-silver dressing. The dressing was changed every other day. The patients' bacteria infection, symptoms, the occurrence of high fever at 7th day and the healing time, recovering rate at 2nd，6th, 10th, 14th, 18th day were compared between the two groups. results: In the observation group, the bacteria infections and the occurrence of high fever at 7th day were significantly lower than the control(P ＜ 0. 05 and P ＜ 0. 01) ，and the healing time and recovering rate at 6th，10th and 14th day were superior to the control group(P ＜ 0. 01) . Conclusions: Nano silver dressing is quite effective in counteracting infection and promoting the wound healing.
Objective: To explore the clinical effect of Nano-silver dressing in treatment of pediatric deep partial-thickness burn. Methods: A total of 80 patients with deep partial thickness burn were randomly divided into two groups，with 40 in each one. The control group was applied with silver zinc cream and the observation group was applied with nano-silver dressing. The dressing was changed every other day. The patients' bacteria infection, symptoms, the occurrence of high fever at 7th day and the healing time, recovering rate at 2nd，6th, 10th, 14th, 18th day were compared between the two groups. results: In the observation group, the bacteria infections and the occurrence of high fever at 7th day were significantly lower than the control(P ＜ 0. 05 and P ＜ 0. 01) ，and the healing time and recovering rate at 6th，10th and 14th day were superior to the control group(P ＜ 0. 01) . Conclusions: Nano silver dressing is quite effective in counteracting infection and promoting the wound healing.
2013, 37(10): 1272-1274.
Abstract:
Objective: To investigate the application value of uterine artery embolization in the treatment of hemorrhagic disease of gynecology and obstetrics. Methods: Thirty-one patients with abnormal hemorrhagic disease of gynecology and obstetrics were treated with uterine artery embolization. results: All uterine artery embolizations of 31 patients were successful, the effects of hemostasis of which were good. The adverse reactions were mild. Conclusions: Uterine artery embolization is an effective therapy means for hemorrhagic disease of gynaecology and obstetrics，which can obtain good curative effects and deserves to be spread and applied in clinic.
Objective: To investigate the application value of uterine artery embolization in the treatment of hemorrhagic disease of gynecology and obstetrics. Methods: Thirty-one patients with abnormal hemorrhagic disease of gynecology and obstetrics were treated with uterine artery embolization. results: All uterine artery embolizations of 31 patients were successful, the effects of hemostasis of which were good. The adverse reactions were mild. Conclusions: Uterine artery embolization is an effective therapy means for hemorrhagic disease of gynaecology and obstetrics，which can obtain good curative effects and deserves to be spread and applied in clinic.
2013, 37(10): 1274-1276.
Abstract:
Objective: To explore the relations of blood glucose with the clinicopathologic features of esophageal carcer and its effects on prognosis. Methods: One hundred sixty-three cases with esophageal carcer were divided into different group according to their clinicopathologic features. The blood glucose levels of all groups were investigated. Fifty-six survival cases were analysed. results: The fasting glucose and fructosamine levels of patients with more than 4 cm in esophageal carcer lesion length were significantly higher than those in cases with less than 4 cm in esophageal carcer lesion length(P ＜ 0. 05) . Compared with patients with stage Ⅱesophageal carcer, the fasting glucose levels of patients with stage Ⅲ increased(P ＜ 0. 05) . For patients with stage Ⅲand Ⅳ esophageal carcer, the survival time of cases with high glucose level were shorter than those in cases with low glucose level(P = 0. 045) . Conclusions: The level of blood glucose is related to the lesion length and stage of esophageal carcer, and which play an important role on the prognosis of middle and terminal esophageal cancer patients.
Objective: To explore the relations of blood glucose with the clinicopathologic features of esophageal carcer and its effects on prognosis. Methods: One hundred sixty-three cases with esophageal carcer were divided into different group according to their clinicopathologic features. The blood glucose levels of all groups were investigated. Fifty-six survival cases were analysed. results: The fasting glucose and fructosamine levels of patients with more than 4 cm in esophageal carcer lesion length were significantly higher than those in cases with less than 4 cm in esophageal carcer lesion length(P ＜ 0. 05) . Compared with patients with stage Ⅱesophageal carcer, the fasting glucose levels of patients with stage Ⅲ increased(P ＜ 0. 05) . For patients with stage Ⅲand Ⅳ esophageal carcer, the survival time of cases with high glucose level were shorter than those in cases with low glucose level(P = 0. 045) . Conclusions: The level of blood glucose is related to the lesion length and stage of esophageal carcer, and which play an important role on the prognosis of middle and terminal esophageal cancer patients.
2013, 37(10): 1277-1279.
Abstract:
Objective: To investigate the relationship between high-sensitivity C-reactive protein(hs-CrP) and carotid intima-media thickness(IMT) in patients with essential hypertension(EH) complicated with cerebral infarction. Methods: Twenty five patients with EH complicated with cerebral infarction(ECI group) ，22 patients with EH(EH group) and 20 healthy people(control group) were selected. The levels of serum hs-CrP, triglyceride, low-density lipoprotein, fasting blood glucose and carotid IMT in three groups were detected. results: The differences of age, sex, smoking history, levels of triglyceride, low density lipoprotein and fasting blood glucose in three groups had no statistical significance(P ＞ 0. 05) . The levels of hs-CrP and carotid IMT of the ECI group were significantly higher than those in control and EH group(P ＜ 0. 05 to P ＜ 0. 01) , the levels of hs-CrP and carotid IMT of the EH group were significantly higher than those in control group(P ＜ 0. 05 and P ＜ 0. 01) . In EH group, the level of hs-CrP in patients with increasing carotid IMT was significantly higher than that in patients with normal carotid IMT, the level of carotid IMT was positively correlated with hs-CrP (P ＜ 0. 01) . The multiple regression analysis showed that carotid IMT was correlated with age and hs-CrP, the regression equation was Y^IMT = 0. 319 + 0. 064Xhs-CrP + 0. 008Xage . Conclusions: hs-CrP may affect the pathogenesis and development of cerebral infarction in ECI patients by promoting the carotid atherosclerosis.
Objective: To investigate the relationship between high-sensitivity C-reactive protein(hs-CrP) and carotid intima-media thickness(IMT) in patients with essential hypertension(EH) complicated with cerebral infarction. Methods: Twenty five patients with EH complicated with cerebral infarction(ECI group) ，22 patients with EH(EH group) and 20 healthy people(control group) were selected. The levels of serum hs-CrP, triglyceride, low-density lipoprotein, fasting blood glucose and carotid IMT in three groups were detected. results: The differences of age, sex, smoking history, levels of triglyceride, low density lipoprotein and fasting blood glucose in three groups had no statistical significance(P ＞ 0. 05) . The levels of hs-CrP and carotid IMT of the ECI group were significantly higher than those in control and EH group(P ＜ 0. 05 to P ＜ 0. 01) , the levels of hs-CrP and carotid IMT of the EH group were significantly higher than those in control group(P ＜ 0. 05 and P ＜ 0. 01) . In EH group, the level of hs-CrP in patients with increasing carotid IMT was significantly higher than that in patients with normal carotid IMT, the level of carotid IMT was positively correlated with hs-CrP (P ＜ 0. 01) . The multiple regression analysis showed that carotid IMT was correlated with age and hs-CrP, the regression equation was Y^IMT = 0. 319 + 0. 064Xhs-CrP + 0. 008Xage . Conclusions: hs-CrP may affect the pathogenesis and development of cerebral infarction in ECI patients by promoting the carotid atherosclerosis.
2013, 37(10): 1280-1281,1284.
Abstract:
Objective: To explore the effects of suboccipital decompression combined with occipital large pool reconstruction in the treatment of Arnold-Chiari typeⅠmalformation(ACM-Ⅰ) complicated with syringomyelia(SM) . Methods: Forty patients were treated with suboccipital decompression through posterior fossa small bone window, improving cerebralspinal fluid circulation and reconstructing occipital large pool under the microscope. results: After operation，MrI showed the cerebellar tonsil was above the level of foramen magnum and cisterna magna expanded. All patients were followed up for 3 to 30 months. No death case, the clinical symptoms improvement in 35 cases and no change in 5 cases were found, the effective rate was 87. 50%. Syringomyelia reduction in 33 cases and no change in 7 cases were found, the effective rate was 82. 50%. Conclusions: Suboccipital decompression combined with occipital large pool reconstruction is an effective way for treating ACM-Ⅰcomplicated with SM.
Objective: To explore the effects of suboccipital decompression combined with occipital large pool reconstruction in the treatment of Arnold-Chiari typeⅠmalformation(ACM-Ⅰ) complicated with syringomyelia(SM) . Methods: Forty patients were treated with suboccipital decompression through posterior fossa small bone window, improving cerebralspinal fluid circulation and reconstructing occipital large pool under the microscope. results: After operation，MrI showed the cerebellar tonsil was above the level of foramen magnum and cisterna magna expanded. All patients were followed up for 3 to 30 months. No death case, the clinical symptoms improvement in 35 cases and no change in 5 cases were found, the effective rate was 87. 50%. Syringomyelia reduction in 33 cases and no change in 7 cases were found, the effective rate was 82. 50%. Conclusions: Suboccipital decompression combined with occipital large pool reconstruction is an effective way for treating ACM-Ⅰcomplicated with SM.
2013, 37(10): 1282-1284.
Abstract:
Objective: To investigate the effects of different endoscopic surgery approaches in the treatment of maxillary sinus benign lesion. Methods: Thirty-nine cases with maxillary sinus benign lesions were treated with four different surgical approaches，which included 18 with the nasal passage to expand natural opening approach，9 cases with double approaches under the middle and inferior nasal passage, 11 cases with enlarging the natural openings of maxillary sinus combined with nasal lateral wall resection approach and 1 case with traditional Caldwell-Luc approach. results: All patients were followed up for 6 to 12 months, 30 cases cured，7 cases improved and 2 cases recurred. One case with inverted papilloma recurred，who was diagnosed with papillary squamous cell carcinoma by pathology after operation and lost to follow-up after radiotherapy. One case with maxillary sinus cyst ocurred in other parts of the maxillary sinus in 8 months after operation，who was untreated because the cyst was small and no clinical symptoms. Conclusions: Different endoscopic surgery approaches can be selected according to the characteristics of maxillary sinus benign lesion，which can improve the clinical effect and reduce surgical trauma.
Objective: To investigate the effects of different endoscopic surgery approaches in the treatment of maxillary sinus benign lesion. Methods: Thirty-nine cases with maxillary sinus benign lesions were treated with four different surgical approaches，which included 18 with the nasal passage to expand natural opening approach，9 cases with double approaches under the middle and inferior nasal passage, 11 cases with enlarging the natural openings of maxillary sinus combined with nasal lateral wall resection approach and 1 case with traditional Caldwell-Luc approach. results: All patients were followed up for 6 to 12 months, 30 cases cured，7 cases improved and 2 cases recurred. One case with inverted papilloma recurred，who was diagnosed with papillary squamous cell carcinoma by pathology after operation and lost to follow-up after radiotherapy. One case with maxillary sinus cyst ocurred in other parts of the maxillary sinus in 8 months after operation，who was untreated because the cyst was small and no clinical symptoms. Conclusions: Different endoscopic surgery approaches can be selected according to the characteristics of maxillary sinus benign lesion，which can improve the clinical effect and reduce surgical trauma.
2013, 37(10): 1285-1287,1290.
Abstract:
Objective: To investigate the expression level of CyclinE and p27 in laryngeal squamous cell carcinoma(LSCC) and their clinical significance. Methods: The expression of CyclinE and p27 in 160 LSCC and 30 normal laryngeal tissues(NLT) were detected by EliVision immunohistochemical method. The relationship between the expression of CyclinE and p27 and clinicopathological parameters of LSCC was analyzed, the relationship between the expression of CyclinE and p27 was analysed. results: The positive rates of CyclinE and p27 expression in LSCC and NLT were 64. 37% ＆ 40. 0% and 16. 67% ＆ 60. 0%, respectively, the difference of which was statistical significance(P ＜ 0. 05 to P ＜ 0. 01) . The expression differences of CyclinE and p27 in differentiation, lymph node metastasis and pTNM stage of LSCC were statistical significance(P ＜ 0. 05 to P ＜ 0. 01) , the expression differences of CyclinE and p27 in age, gender, anatomic location and diameter of tumor were not statistical significance(P ＞ 0. 05) , the expression of CyclinE and p27 was negative relation(P ＜ 0. 05) . Conclusions: The CyclinE and p27 expression difference maybe a molecular basis of development and invasion /metastasis of LSCC. The detection of CyclinE and p27 expression has important significance in predicting the prognosis of patients with LSCC.
Objective: To investigate the expression level of CyclinE and p27 in laryngeal squamous cell carcinoma(LSCC) and their clinical significance. Methods: The expression of CyclinE and p27 in 160 LSCC and 30 normal laryngeal tissues(NLT) were detected by EliVision immunohistochemical method. The relationship between the expression of CyclinE and p27 and clinicopathological parameters of LSCC was analyzed, the relationship between the expression of CyclinE and p27 was analysed. results: The positive rates of CyclinE and p27 expression in LSCC and NLT were 64. 37% ＆ 40. 0% and 16. 67% ＆ 60. 0%, respectively, the difference of which was statistical significance(P ＜ 0. 05 to P ＜ 0. 01) . The expression differences of CyclinE and p27 in differentiation, lymph node metastasis and pTNM stage of LSCC were statistical significance(P ＜ 0. 05 to P ＜ 0. 01) , the expression differences of CyclinE and p27 in age, gender, anatomic location and diameter of tumor were not statistical significance(P ＞ 0. 05) , the expression of CyclinE and p27 was negative relation(P ＜ 0. 05) . Conclusions: The CyclinE and p27 expression difference maybe a molecular basis of development and invasion /metastasis of LSCC. The detection of CyclinE and p27 expression has important significance in predicting the prognosis of patients with LSCC.
2013, 37(10): 1288-1290.
Abstract:
Objective: To investigate the expression of B7-H4 in lymphoma and and its clinical significance. Methods: The expression of B7-H4 in 30 patients with non-Hodgkin lymphoma(NHL) ，7 patients with Hodgkin lymphoma(HL) and 8 patients with benign hyperplastic lymphadenitis were detected using EliVision Plus immunohistochemistry. The correlation of B7-H4 expression with gender, age and disease stage were analyzed by clinical pathology data. results: The positive rate of B7-H4 expression in lymphoma(37 cases) and benign hyperplastic lymphadenitis (8 cases) patients were 54. 1%) and 0. 0%，respectively，the difference of which was statistically significant(P ＜ 0. 05) . The positive rate of B7-H4 expression in NHL and HL patients were 56. 7% and 3 /7, respectively, the difference of which was not statistically significant(P = 0. 680) . The positive rate of B7-H4 expression in stage Ⅰ/Ⅱand Ⅲ/Ⅳ patients were 18. 8% and 81. 0%, respectively, the difference of which was statistically significant(P = 0. 000 2) . The differences of positive rates of B7-H4 expression between gender and age in 37 cases with lymphoma were not statistical significance(P = 0. 330 and P = 0. 746) . Conclusions: The expression of B7-H4 is associated with the pathogenesis of lymphoma，which can be used as an effective index to identify the lymphoma and benign hyperplastic lymphadenitis, and has not clinical value between HL and NHL.
Objective: To investigate the expression of B7-H4 in lymphoma and and its clinical significance. Methods: The expression of B7-H4 in 30 patients with non-Hodgkin lymphoma(NHL) ，7 patients with Hodgkin lymphoma(HL) and 8 patients with benign hyperplastic lymphadenitis were detected using EliVision Plus immunohistochemistry. The correlation of B7-H4 expression with gender, age and disease stage were analyzed by clinical pathology data. results: The positive rate of B7-H4 expression in lymphoma(37 cases) and benign hyperplastic lymphadenitis (8 cases) patients were 54. 1%) and 0. 0%，respectively，the difference of which was statistically significant(P ＜ 0. 05) . The positive rate of B7-H4 expression in NHL and HL patients were 56. 7% and 3 /7, respectively, the difference of which was not statistically significant(P = 0. 680) . The positive rate of B7-H4 expression in stage Ⅰ/Ⅱand Ⅲ/Ⅳ patients were 18. 8% and 81. 0%, respectively, the difference of which was statistically significant(P = 0. 000 2) . The differences of positive rates of B7-H4 expression between gender and age in 37 cases with lymphoma were not statistical significance(P = 0. 330 and P = 0. 746) . Conclusions: The expression of B7-H4 is associated with the pathogenesis of lymphoma，which can be used as an effective index to identify the lymphoma and benign hyperplastic lymphadenitis, and has not clinical value between HL and NHL.
2013, 37(10): 1291-1295.
Abstract:
Objective: To investigate the role of nitric oxide(NO) in reduction of myocardial ischemia-reperfusion(I /r) injury by sufentanil postconditioning in cardiac valve replacement patients under cardiac pulmonary bypass(CPB) . Methods: Sixty patients(ASA grade Ⅱ or Ⅲ，NYHA class Ⅱ or Ⅲ) of both sexes, aged 19-64, scheduled for cardiac valve replacement were randomly divided into 4 groups(n = 15 each): control group(group C) , sufentanil 0. 5 g /kg group(group S1 ) , sufentanil 1. 0 g /kg group(group S2 ) and sufentanil 2. 0 g /kg group(group S3 ) . In groups S1 ，S2 and S3 , sufentanil 0. 5，1 . 0 and 2. 0 g /kg were infused over 2 min via aortic root 5 min before aortic unclamping respectively. In group C, the equal volume of normal saline (2 ml /kg) was infused instead of sufentanil. Blood samples were taken from radial artery before induction of anesthesia(T0 ) and at 2 h(T1 ) ，4 h(T2 ) ，8 h(T3 ) , 24 h (T4 ) and 48 h (T5 ) after aortic unclamping for determination of plasma concentrations of cardiac troponin-I (cTnI) and NO and activities of creatine kinase isoenzyme-MB(CK-MB) and nitric oxide synthase(NOS) . The hemodynamic parameters，duration of CPB, time of aortic clamping, extubation time，duration of stay in ICU, and myocardial contractility score and volume of drainage at 24 h after the operation were recorded. The restoration of spontaneous heart beat were observed. results: The plasma cTnI concentrations and CKMB activity were significantly lower，while the plasma NO concentrations and NOS activity were significantly higher at T1-3 in group S1 than that in group C(P ＜ 0. 01) . The plasma cTnI concentration and CK-MB activity were significantly lower at T1-5 , the plasma NO significantly higher at T3-4 , and the myocardial contractility score at 24 h after the operation was significantly lower in groups S2 ，3 than that in group S1 (P ＜ 0. 05 to P ＜ 0. 01) . There was no significant difference between groups S2 and S3 (P ＞ 0. 05) . Conclusions: Sufentanil postconditioning can relieve myocardial I /r injury in patients undergoing cardiac valve replacement under CPB by activating the NOS /NO pathway，which is dose-dependent and ceiling-effective.
Objective: To investigate the role of nitric oxide(NO) in reduction of myocardial ischemia-reperfusion(I /r) injury by sufentanil postconditioning in cardiac valve replacement patients under cardiac pulmonary bypass(CPB) . Methods: Sixty patients(ASA grade Ⅱ or Ⅲ，NYHA class Ⅱ or Ⅲ) of both sexes, aged 19-64, scheduled for cardiac valve replacement were randomly divided into 4 groups(n = 15 each): control group(group C) , sufentanil 0. 5 g /kg group(group S1 ) , sufentanil 1. 0 g /kg group(group S2 ) and sufentanil 2. 0 g /kg group(group S3 ) . In groups S1 ，S2 and S3 , sufentanil 0. 5，1 . 0 and 2. 0 g /kg were infused over 2 min via aortic root 5 min before aortic unclamping respectively. In group C, the equal volume of normal saline (2 ml /kg) was infused instead of sufentanil. Blood samples were taken from radial artery before induction of anesthesia(T0 ) and at 2 h(T1 ) ，4 h(T2 ) ，8 h(T3 ) , 24 h (T4 ) and 48 h (T5 ) after aortic unclamping for determination of plasma concentrations of cardiac troponin-I (cTnI) and NO and activities of creatine kinase isoenzyme-MB(CK-MB) and nitric oxide synthase(NOS) . The hemodynamic parameters，duration of CPB, time of aortic clamping, extubation time，duration of stay in ICU, and myocardial contractility score and volume of drainage at 24 h after the operation were recorded. The restoration of spontaneous heart beat were observed. results: The plasma cTnI concentrations and CKMB activity were significantly lower，while the plasma NO concentrations and NOS activity were significantly higher at T1-3 in group S1 than that in group C(P ＜ 0. 01) . The plasma cTnI concentration and CK-MB activity were significantly lower at T1-5 , the plasma NO significantly higher at T3-4 , and the myocardial contractility score at 24 h after the operation was significantly lower in groups S2 ，3 than that in group S1 (P ＜ 0. 05 to P ＜ 0. 01) . There was no significant difference between groups S2 and S3 (P ＞ 0. 05) . Conclusions: Sufentanil postconditioning can relieve myocardial I /r injury in patients undergoing cardiac valve replacement under CPB by activating the NOS /NO pathway，which is dose-dependent and ceiling-effective.
2013, 37(10): 1296-1298.
Abstract:
Objective: To explore the effects of taking medicine in different time on the rhythm of blood pressure of elderly patients with hypertension. Methods: One hundred and twenty elderly patients with hypertension were randomly divided into treatment group and control group. The treatment group were treated with felodipine in the morning and enalapril in the evening by oral, the control group were treated with felodipine and enalapril in the morning by oral. The ambulatory blood pressure in two groups were monitored before treatment and after taking medicine 8 weeks, the rhythm of blood pressure of which was compared. results: The blood pressure of both groups before eatment and after taking medicineat 8 weeks were obviously lower than that in taking medicines(P ＜ 0. 01) , the falling range of diastolic pressure at day，blood pressure at night, 24 h SBP and blood pressure in the morning of the treatment group were better than those in control group(P ＜ 0. 05 to P ＜ 0. 01) . The proportion of non dipper type patients in two groups before treatment were 89. 2% and 89. 1%, and 50. 8% and 72. 7% after treatment, respectively. The improvement of rhythm of blood pressure in treatment group was significantly better than that in control group(P ＜ 0. 05) . Conclusions: The different taking medicine styles can significant improve the rhythm of blood pressure and protect target organ of elderly patients with hypertension.
Objective: To explore the effects of taking medicine in different time on the rhythm of blood pressure of elderly patients with hypertension. Methods: One hundred and twenty elderly patients with hypertension were randomly divided into treatment group and control group. The treatment group were treated with felodipine in the morning and enalapril in the evening by oral, the control group were treated with felodipine and enalapril in the morning by oral. The ambulatory blood pressure in two groups were monitored before treatment and after taking medicine 8 weeks, the rhythm of blood pressure of which was compared. results: The blood pressure of both groups before eatment and after taking medicineat 8 weeks were obviously lower than that in taking medicines(P ＜ 0. 01) , the falling range of diastolic pressure at day，blood pressure at night, 24 h SBP and blood pressure in the morning of the treatment group were better than those in control group(P ＜ 0. 05 to P ＜ 0. 01) . The proportion of non dipper type patients in two groups before treatment were 89. 2% and 89. 1%, and 50. 8% and 72. 7% after treatment, respectively. The improvement of rhythm of blood pressure in treatment group was significantly better than that in control group(P ＜ 0. 05) . Conclusions: The different taking medicine styles can significant improve the rhythm of blood pressure and protect target organ of elderly patients with hypertension.
2013, 37(10): 1299-1300.
Abstract:
Objective: To observe the outcomes of pernicious placenta previa pregnancy and neonate. Methods: Two hundred and twenty cases with placenta previa were divided into pernicious placenta previa group(38 cases) and normal placenta previa group(182 cases) . The rate of postpartum hemorrhage，placenta implantation，hysterectomy，cesarean section，premature birth，neonatal asphyxia and neonatal mortality of two groups were compared. results: The differences of the rate of postpartum hemorrhage，placental implantation，hysterectomy, cesarean section，preterm birth，neonatal asphyxia and neonatal death between two groups were statistically significant(P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: The harm of pernicious placenta previa to mother and infant is great. Strengthening pregnancy care and the effective preoperation and operation treatment can improve the prognosis of mother and infant in pernicious placenta previa.
Objective: To observe the outcomes of pernicious placenta previa pregnancy and neonate. Methods: Two hundred and twenty cases with placenta previa were divided into pernicious placenta previa group(38 cases) and normal placenta previa group(182 cases) . The rate of postpartum hemorrhage，placenta implantation，hysterectomy，cesarean section，premature birth，neonatal asphyxia and neonatal mortality of two groups were compared. results: The differences of the rate of postpartum hemorrhage，placental implantation，hysterectomy, cesarean section，preterm birth，neonatal asphyxia and neonatal death between two groups were statistically significant(P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: The harm of pernicious placenta previa to mother and infant is great. Strengthening pregnancy care and the effective preoperation and operation treatment can improve the prognosis of mother and infant in pernicious placenta previa.
2013, 37(10): 1301-1302,1305.
Abstract:
Objective: To study the correlated risk factors of children Henoch-Schonlein purpura nephritis(HSPN) . Methods: One hundred and eighty-six Henoch-Schonlein purpura (HSP) children were reviewed and analyzed. According to the routine urinary examination, children were divided into HSPN group(n = 56) and non-HSPN group(n = 130) , and then monofactorial analysis and nonconditional logistic analysis were performed to evaluate the risk factors in children with HSPN. results: In univariate analysis, frequent occurrence of purprua, arthrosis symptom，hemorrhage of digestive tract and abdominal pain were identified as factors associated with nephritis(P ＜ 0. 05 to P ＜ 0. 01) . Logistic regression analysis showed that nephritis was significantly associated with frequent occurrence of purprua, arthrosis symptom and abdominal pain(P ＜ 0. 01) . Conclusions: Frequent occurrence of purprua，arthrosis symptom and abdominal pain are high factors towards renal damage that can not be neglected. Patients with these risk factors should be rigorously monitored and need to take positive measures to prevent the development and progression of renal damage.
Objective: To study the correlated risk factors of children Henoch-Schonlein purpura nephritis(HSPN) . Methods: One hundred and eighty-six Henoch-Schonlein purpura (HSP) children were reviewed and analyzed. According to the routine urinary examination, children were divided into HSPN group(n = 56) and non-HSPN group(n = 130) , and then monofactorial analysis and nonconditional logistic analysis were performed to evaluate the risk factors in children with HSPN. results: In univariate analysis, frequent occurrence of purprua, arthrosis symptom，hemorrhage of digestive tract and abdominal pain were identified as factors associated with nephritis(P ＜ 0. 05 to P ＜ 0. 01) . Logistic regression analysis showed that nephritis was significantly associated with frequent occurrence of purprua, arthrosis symptom and abdominal pain(P ＜ 0. 01) . Conclusions: Frequent occurrence of purprua，arthrosis symptom and abdominal pain are high factors towards renal damage that can not be neglected. Patients with these risk factors should be rigorously monitored and need to take positive measures to prevent the development and progression of renal damage.
2013, 37(10): 1303-1305.
Abstract:
Objective: To investigate the relationship of abnormal circadian rhythm blood pressure(BP) ，arterial stiffness index and renal damage in patients with primary hypertension. Methods: One hundred and thirteen patients with primary hypertension were divided into non-scoop shape group (66 cases ) and scoop shape group (47 cases ) . The arterial stiffness index，urinary microalbuminuria, 24 h quantity of urinary protein and renal function of two group were detected. The relationships of abnormal circadian rhythm BP, arterial stiffness index and renal damage in patients with primary hypertension were observed. results: The nocturnal systolic and diastolic blood pressure in non-scoop shape group were significantly higher than those in scoop shape group (P ＜ 0. 01) . The differences of arterial stiffness index，pulse pressure and blood pressure circadian rhythm variations in two groups were statistically significant(P ＜ 0. 01) . The differences of blood urea nitrogen, serum cystatin, serum creatinine and microalbuminuria in two groups were statistically significant(P ＜ 0. 05 to P ＜ 0. 01 ) ，but no statistical significance in the difference of blood uric acid (P ＞ 0. 05 ) . Conclusions: There have relationships between abnormal circadian rhythm BP, arterial stiffness index and renal damage in patients with primary hypertension, it is more significant in non-scoop shape group.
Objective: To investigate the relationship of abnormal circadian rhythm blood pressure(BP) ，arterial stiffness index and renal damage in patients with primary hypertension. Methods: One hundred and thirteen patients with primary hypertension were divided into non-scoop shape group (66 cases ) and scoop shape group (47 cases ) . The arterial stiffness index，urinary microalbuminuria, 24 h quantity of urinary protein and renal function of two group were detected. The relationships of abnormal circadian rhythm BP, arterial stiffness index and renal damage in patients with primary hypertension were observed. results: The nocturnal systolic and diastolic blood pressure in non-scoop shape group were significantly higher than those in scoop shape group (P ＜ 0. 01) . The differences of arterial stiffness index，pulse pressure and blood pressure circadian rhythm variations in two groups were statistically significant(P ＜ 0. 01) . The differences of blood urea nitrogen, serum cystatin, serum creatinine and microalbuminuria in two groups were statistically significant(P ＜ 0. 05 to P ＜ 0. 01 ) ，but no statistical significance in the difference of blood uric acid (P ＞ 0. 05 ) . Conclusions: There have relationships between abnormal circadian rhythm BP, arterial stiffness index and renal damage in patients with primary hypertension, it is more significant in non-scoop shape group.
2013, 37(10): 1306-1308,1311.
Abstract:
Objective: To investigate the effects of enteral nutritional suspension on nutritional status and immune function in patients with acute exacerbation of chronic obstructive pulmonary(AECOPD) . Methods: Sixty patients with AECOPD were randomly divided into treatment group(31 cases) and control group(29 cases) . The control group and treatment group were treated with conventional therapy and enteral nutritional suspension based on the control group everyday 1 000 ml, twice a day for 3 weeks, respectively. The clinical effects in two groups were observed. results: Compared with the treatment group before treatment and control group, the nutritional status(including body mass index, skinfold thickness，arm muscle circumference, serum albumin, serum prealbumin, leptin and adiponectin) and immune function(including CD4，CD8，CD4 /CD8 and IgG) of treatment group were improved after treatment (P ＜ 0. 05 to P ＜ 0. 01) , the difference of IgM between two groups was not statistically significant(P ＞ 0. 05) . Conclusions: The effects of enteral nutritional suspension on treating the patients with AECOPD are good，which can improve nutritional status and immune function, to some extend and improve the clinical efficacy.
Objective: To investigate the effects of enteral nutritional suspension on nutritional status and immune function in patients with acute exacerbation of chronic obstructive pulmonary(AECOPD) . Methods: Sixty patients with AECOPD were randomly divided into treatment group(31 cases) and control group(29 cases) . The control group and treatment group were treated with conventional therapy and enteral nutritional suspension based on the control group everyday 1 000 ml, twice a day for 3 weeks, respectively. The clinical effects in two groups were observed. results: Compared with the treatment group before treatment and control group, the nutritional status(including body mass index, skinfold thickness，arm muscle circumference, serum albumin, serum prealbumin, leptin and adiponectin) and immune function(including CD4，CD8，CD4 /CD8 and IgG) of treatment group were improved after treatment (P ＜ 0. 05 to P ＜ 0. 01) , the difference of IgM between two groups was not statistically significant(P ＞ 0. 05) . Conclusions: The effects of enteral nutritional suspension on treating the patients with AECOPD are good，which can improve nutritional status and immune function, to some extend and improve the clinical efficacy.
2013, 37(10): 1309-1311.
Abstract:
Objective: To investigate the feasibility and sensitivity of Montreal cognitive assessment(MoCA) and mini-mental state examination(MMSE) in the evaluation of mild cognitive impairment(MCI) in patients with chronic alcoholism(CA) . Methods: The cognitive function of 60 patients with CA(CA group) and 30 healthy people(control group) were evaluated by MoCA and MMSE. results: The differences of the scores of MoCA and MMSE between CA group and control group were statistically significant(P ＜ 0. 01) . The differences of the visual space and memory and reaction abilities in two groups were statistically significant(P ＜ 0. 01) . Conclusions: The federated applications of MoCA and MMSE are beneficial to find the MCI patients with CA in the early stage. The memory and sensitivity of the impairment in local cognitive domain evaluated by MoCA are better than MMSE. MoCA can provide an evidence for early finding，preventing and treating the cognitive impairment in patients with CA.
Objective: To investigate the feasibility and sensitivity of Montreal cognitive assessment(MoCA) and mini-mental state examination(MMSE) in the evaluation of mild cognitive impairment(MCI) in patients with chronic alcoholism(CA) . Methods: The cognitive function of 60 patients with CA(CA group) and 30 healthy people(control group) were evaluated by MoCA and MMSE. results: The differences of the scores of MoCA and MMSE between CA group and control group were statistically significant(P ＜ 0. 01) . The differences of the visual space and memory and reaction abilities in two groups were statistically significant(P ＜ 0. 01) . Conclusions: The federated applications of MoCA and MMSE are beneficial to find the MCI patients with CA in the early stage. The memory and sensitivity of the impairment in local cognitive domain evaluated by MoCA are better than MMSE. MoCA can provide an evidence for early finding，preventing and treating the cognitive impairment in patients with CA.
2013, 37(10): 1312-1313,1316.
Abstract:
Objective: To observe the efficacy of s-adenosy-L-methione (SAMe ) combined with ursodeoxycholic (UDCA) on intrahepatic cholestasis of pregnancy. Methods: One hundred and sixty-three patients with mild intrahepatic cholestasis of pregnancy were randomly divided into two groups. Group A(n = 85) received oral administration of SAMe 1 000 mg once a day for 16 days combined with oral use of UDCA 300 mg 3 times a day for 20 days; group B(n = 78) received oral administration of UDCA 300 mg 3 times a day for 20 days; both groups were given dexamathasone and calamine. The fasting blood alanine aminotransferase (ALT) , aspartate aminotransferase(AST) , serum total bilirubin(TBiL) ，direct bilirubin(DBiL) and cholyglycine(CG) were examined before and after the therapy; the women's gestational weeks, fetal distress rate，premature birth rate，cesarean section rate，pruritus score and postpartum hemorrhage rate were recorded and compared between the two groups. results: After treatment, the pruritrs score, serum CG, ALT，TBiL and DBiL were significantly different between the two groups(P ＜ 0. 05 to P ＜ 0. 01) . The percentage of premature in group A was remarkably reduced compared with that in group B(P ＜ 0. 05) ; the AST, fetal distress rate，postpartum hemorrhage rate，and cesarean section rate had no significant differences between the two groups(P ＞ 0. 05) . Conclusions: UDCA combined with SAMe is effective in treatment of mild intrahepatic cholestasis of pregnancy.
Objective: To observe the efficacy of s-adenosy-L-methione (SAMe ) combined with ursodeoxycholic (UDCA) on intrahepatic cholestasis of pregnancy. Methods: One hundred and sixty-three patients with mild intrahepatic cholestasis of pregnancy were randomly divided into two groups. Group A(n = 85) received oral administration of SAMe 1 000 mg once a day for 16 days combined with oral use of UDCA 300 mg 3 times a day for 20 days; group B(n = 78) received oral administration of UDCA 300 mg 3 times a day for 20 days; both groups were given dexamathasone and calamine. The fasting blood alanine aminotransferase (ALT) , aspartate aminotransferase(AST) , serum total bilirubin(TBiL) ，direct bilirubin(DBiL) and cholyglycine(CG) were examined before and after the therapy; the women's gestational weeks, fetal distress rate，premature birth rate，cesarean section rate，pruritus score and postpartum hemorrhage rate were recorded and compared between the two groups. results: After treatment, the pruritrs score, serum CG, ALT，TBiL and DBiL were significantly different between the two groups(P ＜ 0. 05 to P ＜ 0. 01) . The percentage of premature in group A was remarkably reduced compared with that in group B(P ＜ 0. 05) ; the AST, fetal distress rate，postpartum hemorrhage rate，and cesarean section rate had no significant differences between the two groups(P ＞ 0. 05) . Conclusions: UDCA combined with SAMe is effective in treatment of mild intrahepatic cholestasis of pregnancy.
2013, 37(10): 1314-1316.
Abstract:
Objective: To investigate the effects of the mosaicplasty with autologous cartilage in the treatment of the cartilaginous lesions of knee joint. Methods: Twenty-three patients were treated with mosaicplasty with autologous cartilage. The clinical effects in all cases were evaluated by Lysholm score, international cartilage repair society and histopathological examination. results: The difference of the excellent rate between before operation and 6 months after operation in all patients had statistical significance (P ＜ 0. 01) . Compare with before operation, the lesion degree was significantly improved(P ＜ 0. 01) . The swelling and pain in 3 cases were found during the following up period，which recovered by articular cavity infusion and lavage. The osteoarthritis，deep infection and deep venous thrombosis were not found. Conclusions: The effects of the mosaicplasty with autologous cartilage in the treatment of the cartilaginous lesions of knee joint are good，which is worthy of being widely applied in clinic.
Objective: To investigate the effects of the mosaicplasty with autologous cartilage in the treatment of the cartilaginous lesions of knee joint. Methods: Twenty-three patients were treated with mosaicplasty with autologous cartilage. The clinical effects in all cases were evaluated by Lysholm score, international cartilage repair society and histopathological examination. results: The difference of the excellent rate between before operation and 6 months after operation in all patients had statistical significance (P ＜ 0. 01) . Compare with before operation, the lesion degree was significantly improved(P ＜ 0. 01) . The swelling and pain in 3 cases were found during the following up period，which recovered by articular cavity infusion and lavage. The osteoarthritis，deep infection and deep venous thrombosis were not found. Conclusions: The effects of the mosaicplasty with autologous cartilage in the treatment of the cartilaginous lesions of knee joint are good，which is worthy of being widely applied in clinic.
2013, 37(10): 1317-1320.
Abstract:
Objective: To investigate the relationship between serum N-terminal pro brain natriuretic peptide(NT-proBNP) levels and left ventricle mass index(LVMI) in maintenance hemodialysis(MHD) patients. Methods: A total of 81 MHD patients were recruited, and the serum NT-proBNP levels were measured，echocardiography was performed and LVMI was calculated. The serum NT-proBNP levels in 55 MHD patients with left ventricular hypertrophy(LVH) according to LVMI were observed dynamically before and after treament of candesartan and levocarnitine for 4 months, the correlation between serum NT-proBNP level and LVMI in these patients was analyzed. results: Both of the serum NT-proBNP level and LVMI in MHD patients were significantly higher than that in control group (P ＜ 0. 01) . The serum NT-proBNP level in MHD patients with LVH was significantly higher than that in the MHD patients without LVH(P ＜ 0. 01) , and was positively correlated with LVMI(P ＜ 0. 01) . After treatment of candesartan and levocarnitine for 4 months, both of the serum NT-proBNP level and LVMI were significantly decreased than before (P ＜ 0. 01) . Conclusions: The serum NTproBNP level was significantly increased in MHD patients，especially in patients with LVH，and was positive correlation with LVMI, serum NT-proBNP level may be used for assessment LVH in MHD patients.
Objective: To investigate the relationship between serum N-terminal pro brain natriuretic peptide(NT-proBNP) levels and left ventricle mass index(LVMI) in maintenance hemodialysis(MHD) patients. Methods: A total of 81 MHD patients were recruited, and the serum NT-proBNP levels were measured，echocardiography was performed and LVMI was calculated. The serum NT-proBNP levels in 55 MHD patients with left ventricular hypertrophy(LVH) according to LVMI were observed dynamically before and after treament of candesartan and levocarnitine for 4 months, the correlation between serum NT-proBNP level and LVMI in these patients was analyzed. results: Both of the serum NT-proBNP level and LVMI in MHD patients were significantly higher than that in control group (P ＜ 0. 01) . The serum NT-proBNP level in MHD patients with LVH was significantly higher than that in the MHD patients without LVH(P ＜ 0. 01) , and was positively correlated with LVMI(P ＜ 0. 01) . After treatment of candesartan and levocarnitine for 4 months, both of the serum NT-proBNP level and LVMI were significantly decreased than before (P ＜ 0. 01) . Conclusions: The serum NTproBNP level was significantly increased in MHD patients，especially in patients with LVH，and was positive correlation with LVMI, serum NT-proBNP level may be used for assessment LVH in MHD patients.
2013, 37(10): 1328-1330.
Abstract:
Objective: To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa and extended-spectrum -lactamase (ESBLs) producing condition, gene distribution and epidemiological characteristics in clinic. Methods: The antimicrobial susceptibility was detected with the Vitek-2 compact automatic bacteria identification system, 16 antibiotics for 208 Pseudomonas aeruginosa isolated. The imipenem-resistant Pseudomonas aeruginosa were selected. ESBLs genes produced by imipenem resistance were detected by PCr, the ESBLs genes contain TEM，SHV，OXA-10，OXA-2，PEr and VEB. results: In the 208 isolates，53 strains of Pseudomonas aeruginosa were resistant to imipenem, the drug resistance rate was 25. 48%. The isolates had a high rate of drug-resistance against CTE，CZ，AMS and FTN; The PCr results showed that in the 53 imipenem-resistant Pseudomonas aeruginosa，ESBLs gene were produced in 23 strains, 16 strains were TEM，4 for OXA-10 and 3 for PEr gene; OXA-2，SHV was not detected. Conclusions: TEM of Extended-spectrum-lactamase producing in imipenem-resistant Pseudomonas aeruginosa is the main gene in our hospital，most of them are multi-drug-resistance，detecting the antimicrobial susceptibility and using antibiotics rationally is essential in clinic.
Objective: To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa and extended-spectrum -lactamase (ESBLs) producing condition, gene distribution and epidemiological characteristics in clinic. Methods: The antimicrobial susceptibility was detected with the Vitek-2 compact automatic bacteria identification system, 16 antibiotics for 208 Pseudomonas aeruginosa isolated. The imipenem-resistant Pseudomonas aeruginosa were selected. ESBLs genes produced by imipenem resistance were detected by PCr, the ESBLs genes contain TEM，SHV，OXA-10，OXA-2，PEr and VEB. results: In the 208 isolates，53 strains of Pseudomonas aeruginosa were resistant to imipenem, the drug resistance rate was 25. 48%. The isolates had a high rate of drug-resistance against CTE，CZ，AMS and FTN; The PCr results showed that in the 53 imipenem-resistant Pseudomonas aeruginosa，ESBLs gene were produced in 23 strains, 16 strains were TEM，4 for OXA-10 and 3 for PEr gene; OXA-2，SHV was not detected. Conclusions: TEM of Extended-spectrum-lactamase producing in imipenem-resistant Pseudomonas aeruginosa is the main gene in our hospital，most of them are multi-drug-resistance，detecting the antimicrobial susceptibility and using antibiotics rationally is essential in clinic.
2013, 37(10): 1331-1333.
Abstract:
Objective: A reversed-phase high performance liquid chromatography(HPLC) method was developed for determination of fluconazole concentration in human serum. Methods: A reversed phase HPLC method was developed after the serum samples were extracted by trichloroacetic acid solvent. HPLC conditions were set as follows: Column: Lichrospher-C18，250 mm 4. 6 mm，5 m; Mobile phase was acetonitrile: water: phosphoric acid(22∶ 78∶ 0. 28, adjusted to pH 4 with ethidene diamine) . Flow rote: 1. 0 ml /min, Detection wave length: 295 nm, the column temperature was 20 ℃. results: Assay linearity was obtained in the range of 0. 1-6 g /ml (r = 0. 999 9) ; the relative standard deviation between intra-day and inter-day was lower than 10%. The recovery rate of three different concentrations(low，middle and high concentration) was between 85. 93% and 100. 60%. Conclusions: The method is rapid, accurate and reproducible. It is suitable for therapeutic drug monitoring.
Objective: A reversed-phase high performance liquid chromatography(HPLC) method was developed for determination of fluconazole concentration in human serum. Methods: A reversed phase HPLC method was developed after the serum samples were extracted by trichloroacetic acid solvent. HPLC conditions were set as follows: Column: Lichrospher-C18，250 mm 4. 6 mm，5 m; Mobile phase was acetonitrile: water: phosphoric acid(22∶ 78∶ 0. 28, adjusted to pH 4 with ethidene diamine) . Flow rote: 1. 0 ml /min, Detection wave length: 295 nm, the column temperature was 20 ℃. results: Assay linearity was obtained in the range of 0. 1-6 g /ml (r = 0. 999 9) ; the relative standard deviation between intra-day and inter-day was lower than 10%. The recovery rate of three different concentrations(low，middle and high concentration) was between 85. 93% and 100. 60%. Conclusions: The method is rapid, accurate and reproducible. It is suitable for therapeutic drug monitoring.
2013, 37(10): 1334-1337.
Abstract:
Objective: To explore the ultrasonic diagnostics features of solitary fibrous tumors (SFT) and their relationships with pathology. Methods: The features of ultrasonic diagnostics，pathomorphology and immunophenotyping of 6 cases with SFT diagnosed by pathology were retrospectively analysed. results: Two cases locating in pelvic cavity，1 case locating in neck，1 case locating in abdominal wall，1 case locating in genal region and 1 case locating in zygomatic region were found. The major clinical manifestations were nonspecific clinical symptoms in lesions. The ultrasonography and color Doppler flow imaging showed hypoechoic area and blood flow sign in the inner of lesion. Fusiform tumor cell sparse area and concentration area and rich interstitial blood vessels with partial expanding were observed in pathology. The positive staining of CD34，CD99，Bcl-2 and Vimentin were detected by immunohistochemistry. Conclusions: SFT with well-encapsulated tumor is the low echo conglomeration with clear boundaries. The lowresistance index of haemodynamics suggests that it maybe malignancy，which can be diagnosed by immunohistochemistry.
Objective: To explore the ultrasonic diagnostics features of solitary fibrous tumors (SFT) and their relationships with pathology. Methods: The features of ultrasonic diagnostics，pathomorphology and immunophenotyping of 6 cases with SFT diagnosed by pathology were retrospectively analysed. results: Two cases locating in pelvic cavity，1 case locating in neck，1 case locating in abdominal wall，1 case locating in genal region and 1 case locating in zygomatic region were found. The major clinical manifestations were nonspecific clinical symptoms in lesions. The ultrasonography and color Doppler flow imaging showed hypoechoic area and blood flow sign in the inner of lesion. Fusiform tumor cell sparse area and concentration area and rich interstitial blood vessels with partial expanding were observed in pathology. The positive staining of CD34，CD99，Bcl-2 and Vimentin were detected by immunohistochemistry. Conclusions: SFT with well-encapsulated tumor is the low echo conglomeration with clear boundaries. The lowresistance index of haemodynamics suggests that it maybe malignancy，which can be diagnosed by immunohistochemistry.
2013, 37(10): 1340-1342.
Abstract:
Objective: To explore the factors influencing the trait coping styles of the college students in Bengbu Universityies. Methods: Questionnaires were used to investigate the mental health status of 1 645 college students from 5 different schools and of different grades. The survey included home-style，home environment and life events; and the outcome was studied by single factor and multiple linear regression analysis. results: The PC scores were significantly different in students of different grades (P ＜ 0. 01) ; mothers' education level affected their PC sores and the difference was significant(P ＜ 0. 01) . The multiple linear regression analysis displayed that NC scores were related with the interpersonal factors，study pressure factors and punishment factors (P ＜ 0. 01 ) . Conclusions: The college students' PC is related to the home environment and the life events stress; corresponding interventions should be taken to improve the students' positive corresponding style.
Objective: To explore the factors influencing the trait coping styles of the college students in Bengbu Universityies. Methods: Questionnaires were used to investigate the mental health status of 1 645 college students from 5 different schools and of different grades. The survey included home-style，home environment and life events; and the outcome was studied by single factor and multiple linear regression analysis. results: The PC scores were significantly different in students of different grades (P ＜ 0. 01) ; mothers' education level affected their PC sores and the difference was significant(P ＜ 0. 01) . The multiple linear regression analysis displayed that NC scores were related with the interpersonal factors，study pressure factors and punishment factors (P ＜ 0. 01 ) . Conclusions: The college students' PC is related to the home environment and the life events stress; corresponding interventions should be taken to improve the students' positive corresponding style.
2013, 37(10): 1351-1353,1357.
Abstract:
Objective: To investigate the relations between the view of employment and the professional commitmentin male nursing students. Methods: The 287 male nursing students from four different colleges were selected，and their view of employment and professional commitment were investigated by a questionnaire. results: The scores of the employment view and the professional commitment of the male nursing students were(78. 19 8. 13) and(82. 53 13. 99) , respectively. The professional commitment level was positively related to the view of employment among the male nursing students(P ＜ 0. 01) . Conclusions: Working enthusiasm, career acknowledgement and positive evaluation are important factors affecting the employment view of the male nursing students.
Objective: To investigate the relations between the view of employment and the professional commitmentin male nursing students. Methods: The 287 male nursing students from four different colleges were selected，and their view of employment and professional commitment were investigated by a questionnaire. results: The scores of the employment view and the professional commitment of the male nursing students were(78. 19 8. 13) and(82. 53 13. 99) , respectively. The professional commitment level was positively related to the view of employment among the male nursing students(P ＜ 0. 01) . Conclusions: Working enthusiasm, career acknowledgement and positive evaluation are important factors affecting the employment view of the male nursing students.
2013, 37(10): 1354-1357.
Abstract:
Objective: To explore the effects of preoperative visiting nursing on psychological status，physiological stress and satisfaction of patients with gynecological tumor. Methods: One hundred and twenty patients with gynecological tumor were randomly divided into observation group and control group (60 cases each group) . The preoperative visiting nursing was implemented in observation group, then two groups were nursed with routine operation nursing. results: The differences of SAS and SDS scores of two groups at 1 day before operation were not statistical significance(P ＞ 0. 05) , the SAS and SDS scores of the observation group at 3 day after operation were obviously lower than those in control group(P ＜ 0. 01) . The systolic pressure，diastolic pressure and heart rate of the observation group at preoperative 5min and skin incision were significantly lower than those in control group (P ＜ 0. 01) . The nursing satisfaction of observation group (98. 3%) was significantly higher than that in control group (88. 3%) (P ＜ 0. 01 ) . Conclusions: The preoperative visiting nursing is a high-quality service，which can effectively improve the psychological status and nursing satisfaction of patients, reduce the physiological stress reactions of patients.
Objective: To explore the effects of preoperative visiting nursing on psychological status，physiological stress and satisfaction of patients with gynecological tumor. Methods: One hundred and twenty patients with gynecological tumor were randomly divided into observation group and control group (60 cases each group) . The preoperative visiting nursing was implemented in observation group, then two groups were nursed with routine operation nursing. results: The differences of SAS and SDS scores of two groups at 1 day before operation were not statistical significance(P ＞ 0. 05) , the SAS and SDS scores of the observation group at 3 day after operation were obviously lower than those in control group(P ＜ 0. 01) . The systolic pressure，diastolic pressure and heart rate of the observation group at preoperative 5min and skin incision were significantly lower than those in control group (P ＜ 0. 01) . The nursing satisfaction of observation group (98. 3%) was significantly higher than that in control group (88. 3%) (P ＜ 0. 01 ) . Conclusions: The preoperative visiting nursing is a high-quality service，which can effectively improve the psychological status and nursing satisfaction of patients, reduce the physiological stress reactions of patients.
2013, 37(10): 1358-1359.
Abstract:
Objective: To explore the effects of health education clinical pathway in the prevention of constipation after cesarean section. Methods: One hundred and fifty-six puerperas were randomly divided into control group and observation group(78 cases each group) . The conventional health education and health education clinical pathway were implemented in control group and observation group, respectively. results: The incidence of constipation in observation group was lower than that in control group(P ＜ 0. 05) , the degree of health knowledge and patient satisfaction in observation group were better than that in control group (P ＜ 0. 01 ) . Conclusions: The health education clinical pathway can reduce the incidence rate of constipation after cesarean section and increase the degree of health knowledge and patient satisfaction.
Objective: To explore the effects of health education clinical pathway in the prevention of constipation after cesarean section. Methods: One hundred and fifty-six puerperas were randomly divided into control group and observation group(78 cases each group) . The conventional health education and health education clinical pathway were implemented in control group and observation group, respectively. results: The incidence of constipation in observation group was lower than that in control group(P ＜ 0. 05) , the degree of health knowledge and patient satisfaction in observation group were better than that in control group (P ＜ 0. 01 ) . Conclusions: The health education clinical pathway can reduce the incidence rate of constipation after cesarean section and increase the degree of health knowledge and patient satisfaction.
2013, 37(10): 1360-1361,1364.
Abstract:
Objective: To evaluate the effect of psychological intervention on phacoemulsification cataract surgery under ocular surface anesthesia. Methods: Fifty-nine cases with senile cataract were performed pacoemusification cataract surgery under surface anesthesia and were divided randomly into two groups: experimental group in which the psychological intervention was performed further and controlled group in which the routine nursing care was given. The process of surgery, cooperation of the patients and pain feeling were recorded and compared in these two groups. results: Seventy-two point four percent of patients went on well, 24. 1% basic smooth and 3. 14% not very well in the cataract surgery in the cataract surgery in the experimental group，and only 56. 7%，33. 3% and 10% respectively in the controlled group，however the difference was not significant(P ＞ 0. 05) . The scores of cooperation in cataract was 4. 69 0. 47 in the experimental group，which was higher significantly in the controlled group whose score was 3. 30 1. 05(P ＜ 0. 01) . Scores of pain feeling was 1. 66 0. 61 in the experimental group and 2. 43 0. 63 in the controlled group, the difference was significan (P ＜ 0. 01) . Conclusions: Psychological intervention can alleviate pain feeling of patients, enhance cooperation and make the cataract surgery go further well.
Objective: To evaluate the effect of psychological intervention on phacoemulsification cataract surgery under ocular surface anesthesia. Methods: Fifty-nine cases with senile cataract were performed pacoemusification cataract surgery under surface anesthesia and were divided randomly into two groups: experimental group in which the psychological intervention was performed further and controlled group in which the routine nursing care was given. The process of surgery, cooperation of the patients and pain feeling were recorded and compared in these two groups. results: Seventy-two point four percent of patients went on well, 24. 1% basic smooth and 3. 14% not very well in the cataract surgery in the cataract surgery in the experimental group，and only 56. 7%，33. 3% and 10% respectively in the controlled group，however the difference was not significant(P ＞ 0. 05) . The scores of cooperation in cataract was 4. 69 0. 47 in the experimental group，which was higher significantly in the controlled group whose score was 3. 30 1. 05(P ＜ 0. 01) . Scores of pain feeling was 1. 66 0. 61 in the experimental group and 2. 43 0. 63 in the controlled group, the difference was significan (P ＜ 0. 01) . Conclusions: Psychological intervention can alleviate pain feeling of patients, enhance cooperation and make the cataract surgery go further well.
2013, 37(10): 1362-1364.
Abstract:
Objective: To explore the appliciation effects of PDCA circulation method in managing the cleaned and disinfected laryngoscope room. Methods: PDCA circulation method was used to collect material, find problem，analyze the reason，make plan, organize and implement and evaluate the effects. The biology condition of 55 cleaned and disinfected laryngoscopes, 50 disinfectants in use, 56 cleaned hands of staff and 50 times of disinfected laryngoscope store room atmosphere were randomly measured before and after using PDCA circulation method. results: The quality of the cleaned and disinfected laryngoscope，disinfectant in use, the cleaned hand of staff and the atmosphere of disinfected laryngoscope store room met the requirement of Technical Specifications of Endoscope Decontamination and Disinfection after using the PDCA circulation method, the total qualified rate of which was 99. 05%. The qualified rate differences of four indexes after using the PDCA cycling method were statistically higher than before (P ＜ 0. 05 ) . Conclusions: The effects of PDCA circulation method in managing the cleaned and disinfected are good，which is an effective way to improve the quality of cleaned and disinfected laryngoscope room.
Objective: To explore the appliciation effects of PDCA circulation method in managing the cleaned and disinfected laryngoscope room. Methods: PDCA circulation method was used to collect material, find problem，analyze the reason，make plan, organize and implement and evaluate the effects. The biology condition of 55 cleaned and disinfected laryngoscopes, 50 disinfectants in use, 56 cleaned hands of staff and 50 times of disinfected laryngoscope store room atmosphere were randomly measured before and after using PDCA circulation method. results: The quality of the cleaned and disinfected laryngoscope，disinfectant in use, the cleaned hand of staff and the atmosphere of disinfected laryngoscope store room met the requirement of Technical Specifications of Endoscope Decontamination and Disinfection after using the PDCA circulation method, the total qualified rate of which was 99. 05%. The qualified rate differences of four indexes after using the PDCA cycling method were statistically higher than before (P ＜ 0. 05 ) . Conclusions: The effects of PDCA circulation method in managing the cleaned and disinfected are good，which is an effective way to improve the quality of cleaned and disinfected laryngoscope room.
2013, 37(10): 1365-1368.
Abstract:
Objective: To investigate the effects of cognitive behavioral nursing therapy on the negative emotions of patients with somatoform disorders. Methods: Sixty-eight patients with somatoform disorder were randomly divided into observation group and control group(34 cases each group) . Two groups were treated with drug therapy and routine nursing, the observation group were additionally treated with cognitive behavior nursing intervention(education intervention) ，psychological intervention and relaxation training, etc. The intervention effects were evaluated by self-evaluation depression scale(SDS) , self-evaluation anxiety scale(SAS) and symptom selfassessment scale(SCL-90) before and after the intervention. results: The differences of SAS，SDS and SCL-90 scores between two groups were not statistically significant before intervention(P ＞ 0. 05) . Twelve weeks after cognitive behavioral nursing therapy, the depression and anxiety levels of the observation group were significantly lower than those in control group and before intervention(P ＜ 0. 01) , the factor scores of somatization，depression and anxiety, interpersonal sensitivity of SCL-90 in observation group were better than those in control group (P ＜ 0. 01) . Conclusions: Cognitive behavioral nursing therapy can significantly improve the depression, anxiety and level of mental health in patients with somatoform disorder, and promote patients recovery.
Objective: To investigate the effects of cognitive behavioral nursing therapy on the negative emotions of patients with somatoform disorders. Methods: Sixty-eight patients with somatoform disorder were randomly divided into observation group and control group(34 cases each group) . Two groups were treated with drug therapy and routine nursing, the observation group were additionally treated with cognitive behavior nursing intervention(education intervention) ，psychological intervention and relaxation training, etc. The intervention effects were evaluated by self-evaluation depression scale(SDS) , self-evaluation anxiety scale(SAS) and symptom selfassessment scale(SCL-90) before and after the intervention. results: The differences of SAS，SDS and SCL-90 scores between two groups were not statistically significant before intervention(P ＞ 0. 05) . Twelve weeks after cognitive behavioral nursing therapy, the depression and anxiety levels of the observation group were significantly lower than those in control group and before intervention(P ＜ 0. 01) , the factor scores of somatization，depression and anxiety, interpersonal sensitivity of SCL-90 in observation group were better than those in control group (P ＜ 0. 01) . Conclusions: Cognitive behavioral nursing therapy can significantly improve the depression, anxiety and level of mental health in patients with somatoform disorder, and promote patients recovery.
Email
RSS