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2013 Vol. 38, No. 11
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2013, 37(11): 1385-1388.
Abstract:
Objective: To obtain the culture filter protein 10( CFP10) gene from Mycobacterium tuberculosis H37Ra and clone it into plasmid for nucleotide sequence analysis. Methods: The genomic DNA from Mycobacterium tuberculosis H37Ra was extracted as template,and the primer was designed according to the CFP10 gene of Mycobacterium tuberculosis H37Ra. The CFP10 gene was amplified from the genome of Mycobacterium tuberculosis H37Ra by polymerase chain reaction( PCR) and cloned into pTG19-T vector. After identification by PCR and restriction enzyme analysis,the CFP10 gene was sequenced,analyzed and compared with that of H37Ra reported in GenBank. Results: The sequencing result showed that the gene was obtained with an open reading frame of 303 bp. Compared with the DNA sequence of Mycobacterium tuberculosis H37Ra,the homology was 100%,which deduced that the amino acid sequence of CFP10 was 100% identity. Conclusions: The CFP10 gene of Mycobacterium tuberculosis H37Ra had been cloned successfully and the homology was 100% compared with Mycobacterium tuberculosis H37Ra.
Objective: To obtain the culture filter protein 10( CFP10) gene from Mycobacterium tuberculosis H37Ra and clone it into plasmid for nucleotide sequence analysis. Methods: The genomic DNA from Mycobacterium tuberculosis H37Ra was extracted as template,and the primer was designed according to the CFP10 gene of Mycobacterium tuberculosis H37Ra. The CFP10 gene was amplified from the genome of Mycobacterium tuberculosis H37Ra by polymerase chain reaction( PCR) and cloned into pTG19-T vector. After identification by PCR and restriction enzyme analysis,the CFP10 gene was sequenced,analyzed and compared with that of H37Ra reported in GenBank. Results: The sequencing result showed that the gene was obtained with an open reading frame of 303 bp. Compared with the DNA sequence of Mycobacterium tuberculosis H37Ra,the homology was 100%,which deduced that the amino acid sequence of CFP10 was 100% identity. Conclusions: The CFP10 gene of Mycobacterium tuberculosis H37Ra had been cloned successfully and the homology was 100% compared with Mycobacterium tuberculosis H37Ra.
2013, 37(11): 1389-1391,1395.
Abstract:
Objective: To investigate the pathological change and expression of malondialdehyde( MDA),superoxide dismutase( SOD),tumor necrosis factor-( TNF-) and interleukin-1( IL-1) in gastrointestinal tissue of rats with acute paraquat( PQ) poisoning. Methods: Forty SD rats were randomly divided into control group and poisoned group( 10 rats and 30 rats,respectively). The control group and poisoned group were disposably perfused with normal saline and 180 mg / kg of the diluent of normal saline and PQ, respectively. The gastrointestinal tissue of control group( 10 rats) and 10 rats of poisoned group at 6,24 and 72 h after treatment were harvested. Gastrointestinal gross morphology was observed. The pathological change,the expression of MDA,SOD,TNF- and IL-1 in gastrointestinal tissue were detected by immunohistochemistry( IHC),and the expression of TNF- and IL-1 in gastrointestinal tissue were detected by ELISA. Results: There were obviously pathological injury in gastrointestinal tissue of rats with acute PQ poisoning. The expressions of MDA,TNF- and IL-1 in poisoned group were significantly higher than those in control group( P 0. 01),the expression of SOD in poisoned group was significantly lower than that in control group( P 0. 01). Conclusions: There are obviously pathological injury in gastrointestinal tissue of rats with acute PQ poisoning. The inflammatory response and oxygen free radicals may play an important role in gastrointestinal injury of poisoned rats.
Objective: To investigate the pathological change and expression of malondialdehyde( MDA),superoxide dismutase( SOD),tumor necrosis factor-( TNF-) and interleukin-1( IL-1) in gastrointestinal tissue of rats with acute paraquat( PQ) poisoning. Methods: Forty SD rats were randomly divided into control group and poisoned group( 10 rats and 30 rats,respectively). The control group and poisoned group were disposably perfused with normal saline and 180 mg / kg of the diluent of normal saline and PQ, respectively. The gastrointestinal tissue of control group( 10 rats) and 10 rats of poisoned group at 6,24 and 72 h after treatment were harvested. Gastrointestinal gross morphology was observed. The pathological change,the expression of MDA,SOD,TNF- and IL-1 in gastrointestinal tissue were detected by immunohistochemistry( IHC),and the expression of TNF- and IL-1 in gastrointestinal tissue were detected by ELISA. Results: There were obviously pathological injury in gastrointestinal tissue of rats with acute PQ poisoning. The expressions of MDA,TNF- and IL-1 in poisoned group were significantly higher than those in control group( P 0. 01),the expression of SOD in poisoned group was significantly lower than that in control group( P 0. 01). Conclusions: There are obviously pathological injury in gastrointestinal tissue of rats with acute PQ poisoning. The inflammatory response and oxygen free radicals may play an important role in gastrointestinal injury of poisoned rats.
2013, 37(11): 1392-1395.
Abstract:
Objective: The aim of the present study was to investigate the expressions of scavenger receptor CD36,CCR7,CD83,CD86 and -opioid receptor( MOR) in dendritic cells( DC) induced by high glucose,then explore the effect of hyperglycemia on atherosclerosis. Methods: Immature dendritic cells( imDC) were extracted from normal human peripheral blood mono-nuclear cells( PBMC) which were isolated and cultured with cytokines including GM-CSF and IL-4. After 7 days,the imDC were separated into 3 group,and differently cultured in RPMI1640 medium with either 5 mmol / L D-glucose( NG group),10 mmol / L glucose( TG group) and 25 mmol / L D-glucose( HG group) for 48 hours,and the expressions of the surface markers( CD83,CD86,CD36 and CCR7) in DC were analyzed by flow cytometry. Results: Compared with NG group,the expressions of CD36,CD83,CD86 and CCR7 were significantly upregulated in TG and HG groups( P 0. 05 to P 0. 01). Compared with NG group,the expression of MOR was significuntly upregulated in HG group( P 0. 05). And compared with TG group,the expressions of CD36,CD83 and CD86 were significantly increased in HG group( P 0. 01). Conclusions: High glucose can promote the expressions of surface molecular CD36,MOR and CCR7 in DC.
Objective: The aim of the present study was to investigate the expressions of scavenger receptor CD36,CCR7,CD83,CD86 and -opioid receptor( MOR) in dendritic cells( DC) induced by high glucose,then explore the effect of hyperglycemia on atherosclerosis. Methods: Immature dendritic cells( imDC) were extracted from normal human peripheral blood mono-nuclear cells( PBMC) which were isolated and cultured with cytokines including GM-CSF and IL-4. After 7 days,the imDC were separated into 3 group,and differently cultured in RPMI1640 medium with either 5 mmol / L D-glucose( NG group),10 mmol / L glucose( TG group) and 25 mmol / L D-glucose( HG group) for 48 hours,and the expressions of the surface markers( CD83,CD86,CD36 and CCR7) in DC were analyzed by flow cytometry. Results: Compared with NG group,the expressions of CD36,CD83,CD86 and CCR7 were significantly upregulated in TG and HG groups( P 0. 05 to P 0. 01). Compared with NG group,the expression of MOR was significuntly upregulated in HG group( P 0. 05). And compared with TG group,the expressions of CD36,CD83 and CD86 were significantly increased in HG group( P 0. 01). Conclusions: High glucose can promote the expressions of surface molecular CD36,MOR and CCR7 in DC.
2013, 37(11): 1396-1398,1401.
Abstract:
Objective: To investigate the effects of chloroquine( CQ) on the proliferation and apoptosis of nasopharyngeal carcinoma cells. Methods: The growth inhibition of CNE-2Z cells induced by different concentration CQ( at 0,2,4,8,16,32 mol / L of CQ) were detected by MTT assay. The effects of different concentration CQ on the colony formation were detected by colony cloning experiment. The apoptosis of CNE-2Z cells induced by CQ was detected by Propyl bromide organism dye. The expression of glucose-regulated protein 78( GRP-78),a marker for endoplasmic reticulum stress,in CNE-2Z cells treated with 10 mol / L of CQ at 0,6,16 and 24 h were detected by Western blot. Results: The different concentration CQ had obviously inhibition effects on CNE-2Z cells. The cell survival rates of CNE-2Z cells treated with 16 mol / L at 24,48 and 72 h were 85. 94%,75. 34% and 56. 45%,respectively. CQ could inhibit the colony formation,induce the apoptosis in a dose-dependent manner and increase the GRP-78 expression of CNE-2Z cells. Conclusions: CQ can inhibit the proliferation of CNE-2Z cells and induce their apoptosis through the overreaction mechanism of endoplasmic reticulum stress.
Objective: To investigate the effects of chloroquine( CQ) on the proliferation and apoptosis of nasopharyngeal carcinoma cells. Methods: The growth inhibition of CNE-2Z cells induced by different concentration CQ( at 0,2,4,8,16,32 mol / L of CQ) were detected by MTT assay. The effects of different concentration CQ on the colony formation were detected by colony cloning experiment. The apoptosis of CNE-2Z cells induced by CQ was detected by Propyl bromide organism dye. The expression of glucose-regulated protein 78( GRP-78),a marker for endoplasmic reticulum stress,in CNE-2Z cells treated with 10 mol / L of CQ at 0,6,16 and 24 h were detected by Western blot. Results: The different concentration CQ had obviously inhibition effects on CNE-2Z cells. The cell survival rates of CNE-2Z cells treated with 16 mol / L at 24,48 and 72 h were 85. 94%,75. 34% and 56. 45%,respectively. CQ could inhibit the colony formation,induce the apoptosis in a dose-dependent manner and increase the GRP-78 expression of CNE-2Z cells. Conclusions: CQ can inhibit the proliferation of CNE-2Z cells and induce their apoptosis through the overreaction mechanism of endoplasmic reticulum stress.
2013, 37(11): 1399-1401.
Abstract:
Objective: To explore the expression and clinical significance of survival protein recombinant retroviral vector construction in diseases. Methods: One hundred and twenty healthy mice with close body weight were chosen by using random single-blind research method. The mice were given aristolochic acid to induce lymphocytoma,which was extracted for cell culture in vitro. In the control group,each mouse's lymphoma cell nutrient solution was added conventional normal antineoplastic drugs,while in the observation group it was added the dendritic cell nutrient protein recombinant retroviral vector. The cancer cell death and anticancer conditions in the lymphoma cell nutrient solution were compared and analyzed between the two groups. Results: In the observation group,The death rates for the cancer cell culture fluid with 60%- 79% and 80%- 100% oncocyte were 40. 83% and 29. 17% respectively,while they were 14. 17% and 3. 33% in the control group. The difference was significant( P 0. 01). The cell culture fluid of the observation group was significantly better than that of the control group in eliminating oncocyte with a total efficiency of 95. 83%,which was significantly higher than that of the control group( 51. 67%)( P 0. 01). Conclusions: The dendritic cells infected by the survival protein recombinant retroviral vector can effectively restrain the lymphoma cells growth and reproduction in some degree,and antagonize the lymphoma cell. They have certain positive meaning in the treatment of lymphocytoma.
Objective: To explore the expression and clinical significance of survival protein recombinant retroviral vector construction in diseases. Methods: One hundred and twenty healthy mice with close body weight were chosen by using random single-blind research method. The mice were given aristolochic acid to induce lymphocytoma,which was extracted for cell culture in vitro. In the control group,each mouse's lymphoma cell nutrient solution was added conventional normal antineoplastic drugs,while in the observation group it was added the dendritic cell nutrient protein recombinant retroviral vector. The cancer cell death and anticancer conditions in the lymphoma cell nutrient solution were compared and analyzed between the two groups. Results: In the observation group,The death rates for the cancer cell culture fluid with 60%- 79% and 80%- 100% oncocyte were 40. 83% and 29. 17% respectively,while they were 14. 17% and 3. 33% in the control group. The difference was significant( P 0. 01). The cell culture fluid of the observation group was significantly better than that of the control group in eliminating oncocyte with a total efficiency of 95. 83%,which was significantly higher than that of the control group( 51. 67%)( P 0. 01). Conclusions: The dendritic cells infected by the survival protein recombinant retroviral vector can effectively restrain the lymphoma cells growth and reproduction in some degree,and antagonize the lymphoma cell. They have certain positive meaning in the treatment of lymphocytoma.
2013, 37(11): 1402-1405.
Abstract:
Objective: To explore the surgical treatment of hilar cholangiocarcinoma and its prognosis factors. Methods: The clinical data of 103 cases with hilar cholangiocarcinoma were analyzed retrospectively. Among 103 cases,28 cases were treated with radical resection,12 cases were treated with palliative resection,34 cases were treated with percutaneous transhepatic cholangial drainage( PTCD) combined with percutaneous biliary stent implantation and 29 cases were treated with PTCD. Results: Their median survival time were( 26. 00 1. 51),( 16. 00 1. 49),( 12. 00 1. 83) and( 5. 00 1. 49) months,respectively,the time differences had statistical significance( P 0. 01). The Bismuth type,lymph node metastasis and intraoperative chemotherapy affected the prognosis of patients with hilar cholangiocarcinoma. Conclusions: Radica resection is the best treatment for hilar cholangiocarcinoma. The Bismuth type,lymph node metastasis and intraoperative chemotherapy are important influence factors for the prognosis.
Objective: To explore the surgical treatment of hilar cholangiocarcinoma and its prognosis factors. Methods: The clinical data of 103 cases with hilar cholangiocarcinoma were analyzed retrospectively. Among 103 cases,28 cases were treated with radical resection,12 cases were treated with palliative resection,34 cases were treated with percutaneous transhepatic cholangial drainage( PTCD) combined with percutaneous biliary stent implantation and 29 cases were treated with PTCD. Results: Their median survival time were( 26. 00 1. 51),( 16. 00 1. 49),( 12. 00 1. 83) and( 5. 00 1. 49) months,respectively,the time differences had statistical significance( P 0. 01). The Bismuth type,lymph node metastasis and intraoperative chemotherapy affected the prognosis of patients with hilar cholangiocarcinoma. Conclusions: Radica resection is the best treatment for hilar cholangiocarcinoma. The Bismuth type,lymph node metastasis and intraoperative chemotherapy are important influence factors for the prognosis.
2013, 37(11): 1405-1407.
Abstract:
Objective: To compare the diagnostic value of MoCA and MMSE in subcortical ischemic vascular disease( SIVD). Methods: In 98 cases with vascular cognitive impairment( vMCI),50 cases( MoCA-M group) and 48 cases( MMSE-M group) were measuured by MoCA and MMSE,respectively. In 85 control cases,46 cases( MoCA-C group) and 39 cases( MMSE-C group) were measured by MoCA and MMSE,respectively. Results: The sensitivity of MoCA-M group( 78. 0%) in patients with vMCI was significantly higher than that of MMSE-M group( 52. 1%)( P 0. 01). The specificity of MoCA-M group( 71. 7%) in patients with vMCI was higher than that of MMSE-M group( 48. 7%)( P 0. 05). Conclusions: MoCA has high sensitivity and specificity for detecting vMCI,which may become a effective way for the early diagnosis of SIVD in clinic.
Objective: To compare the diagnostic value of MoCA and MMSE in subcortical ischemic vascular disease( SIVD). Methods: In 98 cases with vascular cognitive impairment( vMCI),50 cases( MoCA-M group) and 48 cases( MMSE-M group) were measuured by MoCA and MMSE,respectively. In 85 control cases,46 cases( MoCA-C group) and 39 cases( MMSE-C group) were measured by MoCA and MMSE,respectively. Results: The sensitivity of MoCA-M group( 78. 0%) in patients with vMCI was significantly higher than that of MMSE-M group( 52. 1%)( P 0. 01). The specificity of MoCA-M group( 71. 7%) in patients with vMCI was higher than that of MMSE-M group( 48. 7%)( P 0. 05). Conclusions: MoCA has high sensitivity and specificity for detecting vMCI,which may become a effective way for the early diagnosis of SIVD in clinic.
2013, 37(11): 1408-1409.
Abstract:
Objective: To investigate the impact of lymph node metastasis on the prognosis of patients with stage Ⅲ c ovarian carcinoma. Methods: Forty-one patients with stage Ⅲc ovarian cancer from 2003 to 2010 were analyzed retrospectively. All the patients had received line specification surgery and enough cycles of postoperative chemotherapy. Twelve cases had simple lymph node metastasis and 29 intra-abdominal peritoneal tumors 2 cm in diameter without or with lymph node metastasis; 25 cases had no postoperative residual or residual tumors 1 cm in size,and 16 had residual tumors 1 cm in size. Results: The follow-up ranged from 2 to 90 months. The 3-year overall survival( OS) and the 5-year OS in patients with simple lymph node metastasis were 83. 3% and 75. 0% respectively; whereas the 3-year and 5-year OS in cases with intra-abdominal peritoneal tumors 2 cm in diameter without or with lymph node metastasis were 58. 6% and 37. 9%,respectively. The 5-year OS was statistically different( P 0. 05); The patients with no postoperative residual or residual tumors 1 cm in size had significantly better outcomes; and their 3-year OS and 5-year OS were 80. 0% and 56. 0% respectively,which were significantly higher than those with residual tumors 1 cm in size( P 0. 05). Conclusions: Patients of stage Ⅲc ovarian carcinoma with simple lymph node metastasis have a better prognosis than those with intra-abdominal peritoneal tumors 2 cm in diameter. The size of residual tumor is the relevant prognostic factor for patients with stage Ⅲc ovarian carcinoma.
Objective: To investigate the impact of lymph node metastasis on the prognosis of patients with stage Ⅲ c ovarian carcinoma. Methods: Forty-one patients with stage Ⅲc ovarian cancer from 2003 to 2010 were analyzed retrospectively. All the patients had received line specification surgery and enough cycles of postoperative chemotherapy. Twelve cases had simple lymph node metastasis and 29 intra-abdominal peritoneal tumors 2 cm in diameter without or with lymph node metastasis; 25 cases had no postoperative residual or residual tumors 1 cm in size,and 16 had residual tumors 1 cm in size. Results: The follow-up ranged from 2 to 90 months. The 3-year overall survival( OS) and the 5-year OS in patients with simple lymph node metastasis were 83. 3% and 75. 0% respectively; whereas the 3-year and 5-year OS in cases with intra-abdominal peritoneal tumors 2 cm in diameter without or with lymph node metastasis were 58. 6% and 37. 9%,respectively. The 5-year OS was statistically different( P 0. 05); The patients with no postoperative residual or residual tumors 1 cm in size had significantly better outcomes; and their 3-year OS and 5-year OS were 80. 0% and 56. 0% respectively,which were significantly higher than those with residual tumors 1 cm in size( P 0. 05). Conclusions: Patients of stage Ⅲc ovarian carcinoma with simple lymph node metastasis have a better prognosis than those with intra-abdominal peritoneal tumors 2 cm in diameter. The size of residual tumor is the relevant prognostic factor for patients with stage Ⅲc ovarian carcinoma.
2013, 37(11): 1410-1412.
Abstract:
Objective: To investigate the value of serum procalcitonin( PCT) in the diagnosis of bacterial infection. Methods: The results of PCT,white blood cell( WBC) and C reactive protein( CRP) in 36 cases with positive bacterial culture and 40 cases with negative bacterial culture were analyzed retrospectively. The PCT was detected by semi-quantitative immunochromatography. Results: There was no statistical difference in the grading of PCT between the two groups( P 0. 05). The mean WBC and CRP in the positive bacterial culture group were significantly higher than those in the negative bacterial culture group( P 0. 01). The positive rate of PCT, WBC and CRP was 36. 11%,63. 89% and 97. 22% respectively in the positive bacterial culture group,and 25. 00%,22. 50% and 72. 50% respectively in the negative bacterial culture group. The positive rate of WBC and CRP in the positive bacterial culture group was apparently higher than that in the negative bacterial culture group( P 0. 01). There was no statistical difference in the positive rate of PCT between the two groups( P 0. 05). The sensitivity of PCT was 36. 11%,WBC 63. 89% and CRP 97. 22%; the specificity of PCT was 75. 00%,WBC 77. 50% and CRP 27. 50%,respectively. Conclusions: Serum PCT is of value in diagnosis of bacterial infection. Combined determination of PCT,WBC and CRP may help to make accurate diagnosis of bacterial infection.
Objective: To investigate the value of serum procalcitonin( PCT) in the diagnosis of bacterial infection. Methods: The results of PCT,white blood cell( WBC) and C reactive protein( CRP) in 36 cases with positive bacterial culture and 40 cases with negative bacterial culture were analyzed retrospectively. The PCT was detected by semi-quantitative immunochromatography. Results: There was no statistical difference in the grading of PCT between the two groups( P 0. 05). The mean WBC and CRP in the positive bacterial culture group were significantly higher than those in the negative bacterial culture group( P 0. 01). The positive rate of PCT, WBC and CRP was 36. 11%,63. 89% and 97. 22% respectively in the positive bacterial culture group,and 25. 00%,22. 50% and 72. 50% respectively in the negative bacterial culture group. The positive rate of WBC and CRP in the positive bacterial culture group was apparently higher than that in the negative bacterial culture group( P 0. 01). There was no statistical difference in the positive rate of PCT between the two groups( P 0. 05). The sensitivity of PCT was 36. 11%,WBC 63. 89% and CRP 97. 22%; the specificity of PCT was 75. 00%,WBC 77. 50% and CRP 27. 50%,respectively. Conclusions: Serum PCT is of value in diagnosis of bacterial infection. Combined determination of PCT,WBC and CRP may help to make accurate diagnosis of bacterial infection.
2013, 37(11): 1412-1413.
Abstract:
Objective: To evaluate the clinical diagnosis value of endoscopic miniprobe sonography( MPS) on prominence lesions of upper gastrointestinal tract. Methods: MPS was performed on 53 prominence lesions of upper gastrointestinal tract of 46 patients. Results: Twenty-two cases were polypuse,12 cases were interstitialoma,4 cases were lipoma,3 cases were heterotopic pancrease,3 cases were cyst,3 cases were external pressure change,2 cases were esophageal varix,2 cases were prolapse of gastric mucosa,1 case was early cancer located in gastric antrum,and 1 case was free of disease. The diagnostic accordance rate of MPS was 100% with the postoperative diagnosis in some lesions. Conclusions: MPS is an accurate,safe,convenient and noninvasive method to diagnose the prominence lesions of upper gastrointestinal tract. It is very important for treatment and evaluating prognosis.
Objective: To evaluate the clinical diagnosis value of endoscopic miniprobe sonography( MPS) on prominence lesions of upper gastrointestinal tract. Methods: MPS was performed on 53 prominence lesions of upper gastrointestinal tract of 46 patients. Results: Twenty-two cases were polypuse,12 cases were interstitialoma,4 cases were lipoma,3 cases were heterotopic pancrease,3 cases were cyst,3 cases were external pressure change,2 cases were esophageal varix,2 cases were prolapse of gastric mucosa,1 case was early cancer located in gastric antrum,and 1 case was free of disease. The diagnostic accordance rate of MPS was 100% with the postoperative diagnosis in some lesions. Conclusions: MPS is an accurate,safe,convenient and noninvasive method to diagnose the prominence lesions of upper gastrointestinal tract. It is very important for treatment and evaluating prognosis.
2013, 37(11): 1414-1417.
Abstract:
Objective: To investigate the risk factors of the delayed gastric emptying( DGE) after child pancreaticojejunostomy( PD). Methods: The clinical data of 132 patients with PD were retrospectively analyzed from 2002 to 2012. Results: The postoperative DGE in 7 cases were found,the incidence rate of which was 35. 61%. The differences of DGE in gender,age,hypertension,diabetes mellitus, direct bilirubin levels,operative time and early postoperative enteral nutrition had no statistical significance( P 0. 05). The preoperative white blood cell levels,ALB levels,intraoperative blood loss and postoperative pancreatic fistula had effects on the incidence of DGE( P 0. 05 to P 0. 01). The multivariate analysis showed ALB and DGE was negatively correlated( P 0. 05),the value of OR was 0. 797. The intraoperative blood loss,operation time,postoperative pancreatic fistula were positively correlated with DGE( P 0. 01),the value of OR were 2. 275,1. 756 and 2. 885,respectively. Conclusions: Improving nutritional status,reducing blood loss,shortening the operation time can reduce the occurrence of DGE,postoperative pancreatic fistula can increase the incidence of DGE.
Objective: To investigate the risk factors of the delayed gastric emptying( DGE) after child pancreaticojejunostomy( PD). Methods: The clinical data of 132 patients with PD were retrospectively analyzed from 2002 to 2012. Results: The postoperative DGE in 7 cases were found,the incidence rate of which was 35. 61%. The differences of DGE in gender,age,hypertension,diabetes mellitus, direct bilirubin levels,operative time and early postoperative enteral nutrition had no statistical significance( P 0. 05). The preoperative white blood cell levels,ALB levels,intraoperative blood loss and postoperative pancreatic fistula had effects on the incidence of DGE( P 0. 05 to P 0. 01). The multivariate analysis showed ALB and DGE was negatively correlated( P 0. 05),the value of OR was 0. 797. The intraoperative blood loss,operation time,postoperative pancreatic fistula were positively correlated with DGE( P 0. 01),the value of OR were 2. 275,1. 756 and 2. 885,respectively. Conclusions: Improving nutritional status,reducing blood loss,shortening the operation time can reduce the occurrence of DGE,postoperative pancreatic fistula can increase the incidence of DGE.
2013, 37(11): 1417-1419.
Abstract:
Objective: To compare the clinical effects of proximal femoral intramedullary nails( PFNA) and minimally invasive percutaneous plate osteosynthesis( MIPPO) in the treatment of intertrochanteric fracture. Methods: Sixty-five cases with intertrochanteric fracture were treated with PFNA( group PFNA) and MIPPO( group MIPPO). The clinical data of operation period index,clinical healing time of fracture,the recovery degree of postoperative hip joint function and postoperative complications of two groups were retrospectively analysed. Results: All patients were followed up for 8 to 25 months. There were not statistical significance in the time of fracture healing and recovery degree of postoperative hip joint function between two groups( P 0. 05). The time of operation and lying in bed,incision length,intraoperative blood loss and hospitalization days of PFNA group were less than those of MIPPO group( P 0. 01). Conclusions: The clinical effects of two kinds of fixed means are similar. PFNA is smaller trauma,less bleeding and shorter time in bed.
Objective: To compare the clinical effects of proximal femoral intramedullary nails( PFNA) and minimally invasive percutaneous plate osteosynthesis( MIPPO) in the treatment of intertrochanteric fracture. Methods: Sixty-five cases with intertrochanteric fracture were treated with PFNA( group PFNA) and MIPPO( group MIPPO). The clinical data of operation period index,clinical healing time of fracture,the recovery degree of postoperative hip joint function and postoperative complications of two groups were retrospectively analysed. Results: All patients were followed up for 8 to 25 months. There were not statistical significance in the time of fracture healing and recovery degree of postoperative hip joint function between two groups( P 0. 05). The time of operation and lying in bed,incision length,intraoperative blood loss and hospitalization days of PFNA group were less than those of MIPPO group( P 0. 01). Conclusions: The clinical effects of two kinds of fixed means are similar. PFNA is smaller trauma,less bleeding and shorter time in bed.
2013, 37(11): 1420-1421,1424.
Abstract:
Objective: To investigate the efficacy and safety of tirofiban in the treatment of patients receiving primary percutaneous coronary intervention( PCI) for acute ST-segment elevation myocardial infarction( STEMI). Methods: The clinical data of 100 patients with acute STEMI were analyzed retrospectively. Fifty of them( control group) received dual antiplatelet therapy( DAPT)( aspirin and clopidogrel) before and after primary PCI,and the other fifty( experimental group) received trigeminy antiplatelet therapy( aspirin, clopidogrel and tirofiban) till 48 hours after primary PCI. Results: At 30 days,the incidence of major adverse cardiovascular events includingcardiac death,non-fetal MI,target vessel revascularization and severe unstable angina in the experimental group was 4. 0%, which was significantly lower than the 18. 0% in the control group( P 0. 05). From 48 hours up to 30 days,the incidence of minor bleeding and thrombocytopenia had no significant difference between the two groups( P 0. 05). No major bleeding occurred in either group. Conclusions: Adding tirofiban to DAPT could safely decrease the incidence of 30 day MACE in patients with STEMI undergoing primary PCI.
Objective: To investigate the efficacy and safety of tirofiban in the treatment of patients receiving primary percutaneous coronary intervention( PCI) for acute ST-segment elevation myocardial infarction( STEMI). Methods: The clinical data of 100 patients with acute STEMI were analyzed retrospectively. Fifty of them( control group) received dual antiplatelet therapy( DAPT)( aspirin and clopidogrel) before and after primary PCI,and the other fifty( experimental group) received trigeminy antiplatelet therapy( aspirin, clopidogrel and tirofiban) till 48 hours after primary PCI. Results: At 30 days,the incidence of major adverse cardiovascular events includingcardiac death,non-fetal MI,target vessel revascularization and severe unstable angina in the experimental group was 4. 0%, which was significantly lower than the 18. 0% in the control group( P 0. 05). From 48 hours up to 30 days,the incidence of minor bleeding and thrombocytopenia had no significant difference between the two groups( P 0. 05). No major bleeding occurred in either group. Conclusions: Adding tirofiban to DAPT could safely decrease the incidence of 30 day MACE in patients with STEMI undergoing primary PCI.
2013, 37(11): 1422-1424.
Abstract:
Objective: To investigate the etiology and clinical features of hypoglycemic encephalopathy. Methods: The etiology and clinical features of 56 patients with hypoglycemic encephalopathy were retrospectively analysed. Results: Among 56 patients with hypoglycemic encephalopathy,39 cases were related to hypoglycemic drugs,5 cases were caused by the fluoroquinolone drugs,4 cases were caused by insufficient food,3 cases were caused by unknown components and health products,the causes of 3 cases were unknown and 2 cases were complicated with upper respiratory tract infection. The clinical features were disturbance of consciousness,insanity, epilepsy,hemiplegia and choreic movement. Conclusions: The etiology and clinical features of hypoglycemia encephalopathy are complicated and lack of specificity,which is ease to misdiagnose. The hypoglycemic encephalopathy in the patients with central nervous system damage should be taken into account.
Objective: To investigate the etiology and clinical features of hypoglycemic encephalopathy. Methods: The etiology and clinical features of 56 patients with hypoglycemic encephalopathy were retrospectively analysed. Results: Among 56 patients with hypoglycemic encephalopathy,39 cases were related to hypoglycemic drugs,5 cases were caused by the fluoroquinolone drugs,4 cases were caused by insufficient food,3 cases were caused by unknown components and health products,the causes of 3 cases were unknown and 2 cases were complicated with upper respiratory tract infection. The clinical features were disturbance of consciousness,insanity, epilepsy,hemiplegia and choreic movement. Conclusions: The etiology and clinical features of hypoglycemia encephalopathy are complicated and lack of specificity,which is ease to misdiagnose. The hypoglycemic encephalopathy in the patients with central nervous system damage should be taken into account.
2013, 37(11): 1425-1427,1430.
Abstract:
Objective: To evaluate the effects of the fiber post system in the repair of residual crowns and roots of incisor. Methods: Fifty-two incisor teeth in 30 patients were treated with oral examinatin,removing the bad restoration,completing root canal therapy, periodontical treatment and necessary surgery. The patients with healthy periodontal condition and without discomfort within one week were prepared,then they were treated with tooth preparation combined with all-ceramic crown restoration according to the principle of full crown preparation( including trying to save the health of dentin,sticking post and nucleation). Thirty patients were followed up for 6 to 12 months,the effects were evaluated by radiology and clinical examination. Results: Post detaching in 1 case and post fracture and gingival congestion in 1 case were found,the good restoration margin,stable colour,good healthy gingiva,no abnormal root and post and secondary periapical paradentitis in other patients were found. Conclusions: The effects of fiber post on repairing the residual roots and crowns of incisor teeth are good. The fiber post should be carefully selected in the treatment of root canal with less residual tooth tissue and no enough dentin shoulder collar.
Objective: To evaluate the effects of the fiber post system in the repair of residual crowns and roots of incisor. Methods: Fifty-two incisor teeth in 30 patients were treated with oral examinatin,removing the bad restoration,completing root canal therapy, periodontical treatment and necessary surgery. The patients with healthy periodontal condition and without discomfort within one week were prepared,then they were treated with tooth preparation combined with all-ceramic crown restoration according to the principle of full crown preparation( including trying to save the health of dentin,sticking post and nucleation). Thirty patients were followed up for 6 to 12 months,the effects were evaluated by radiology and clinical examination. Results: Post detaching in 1 case and post fracture and gingival congestion in 1 case were found,the good restoration margin,stable colour,good healthy gingiva,no abnormal root and post and secondary periapical paradentitis in other patients were found. Conclusions: The effects of fiber post on repairing the residual roots and crowns of incisor teeth are good. The fiber post should be carefully selected in the treatment of root canal with less residual tooth tissue and no enough dentin shoulder collar.
2013, 37(11): 1428-1430.
Abstract:
Objective: To explore the significance of high-risk human papilloma virus( HR-HPV) detection in cervical cancer screening. Methods: Two thousand four hundred and forty-two patients were detected using liquid base cytology( TCT),2 147 patients were detected by HPV hybrid capture method of the second generation( HPV-HC Ⅱ) or nucleic acid molecular hybridization technique,TCT and HPV testing of which 783 patients were simultaneously detected. HR-HPV testing in 723 cases with normal or abnormal cytology were positive,the patients with normal cytology,negative HR-HPV and clinical highly suspected lesions were treated with multipoint biopsy under colposcope and cervical electric ring resection( LEEP),these data combined with pathological results were analyzed. Results: Amony samples with TCT and HPV testing,the HR-HPV positive rates in normal or inflammation TCT,ASC-US,lowgrade squamous intraepithelial lesion and high-grade squmous intraepithelial lesion were 22. 39%,48. 09%,74. 47% and 88. 46%, respectively,the difference of which was statistical significance( P 0. 01). The HR-HPV positive rate increased significantly with the increasimg of cytology diagnosis level. LEEP pathological results showed the HR-HPV positive rates in normal or inflammation tissue, cervical intraepithelial neoplasia( CIN) 1,CIN 2,CIN 3,squamous cell carcinoma and condyloma were 23. 72%,83. 87%,85. 19%, 88. 10%,92. 86% and 72. 37%,respectively,the difference of which was statistical significant( P 0. 01). Conclusions: The continuous HR-HPV positive is related to the evolution of cervical lesions,the HR-HPV detection has important significant in screening cervical cancer.
Objective: To explore the significance of high-risk human papilloma virus( HR-HPV) detection in cervical cancer screening. Methods: Two thousand four hundred and forty-two patients were detected using liquid base cytology( TCT),2 147 patients were detected by HPV hybrid capture method of the second generation( HPV-HC Ⅱ) or nucleic acid molecular hybridization technique,TCT and HPV testing of which 783 patients were simultaneously detected. HR-HPV testing in 723 cases with normal or abnormal cytology were positive,the patients with normal cytology,negative HR-HPV and clinical highly suspected lesions were treated with multipoint biopsy under colposcope and cervical electric ring resection( LEEP),these data combined with pathological results were analyzed. Results: Amony samples with TCT and HPV testing,the HR-HPV positive rates in normal or inflammation TCT,ASC-US,lowgrade squamous intraepithelial lesion and high-grade squmous intraepithelial lesion were 22. 39%,48. 09%,74. 47% and 88. 46%, respectively,the difference of which was statistical significance( P 0. 01). The HR-HPV positive rate increased significantly with the increasimg of cytology diagnosis level. LEEP pathological results showed the HR-HPV positive rates in normal or inflammation tissue, cervical intraepithelial neoplasia( CIN) 1,CIN 2,CIN 3,squamous cell carcinoma and condyloma were 23. 72%,83. 87%,85. 19%, 88. 10%,92. 86% and 72. 37%,respectively,the difference of which was statistical significant( P 0. 01). Conclusions: The continuous HR-HPV positive is related to the evolution of cervical lesions,the HR-HPV detection has important significant in screening cervical cancer.
2013, 37(11): 1431-1433.
Abstract:
Objective: To explore the effects of compound lithospermum ointment in the treatment of middle and small small area burn. Methods: One hundred and twenty patients with burn were randomly divided into control group and observation group( 60 cases each group). The control group and observation group were treated with Mabel burn cream and compound lithospermum ointment, respectively. Results: The cure rate in the observation group( 95. 0%) was higher than that in the control group( 80. 0%)( P 0. 05). The healing time of observation group with shallow Ⅱ wound( 9. 2 3. 1) d was shorter than that in control group( 15. 3 3. 2) d( P 0. 01). No obvious scar in the healing wound in two groups was found. The healing time of observation group with deep Ⅱ wound( 15. 3 3. 2) d was shorter than that in control group( 20 2. 6) d( P 0. 01). Conclusions: Compound lithospermum ointment in the treatment of middle and small small area burn is high cure rate and short healing time.
Objective: To explore the effects of compound lithospermum ointment in the treatment of middle and small small area burn. Methods: One hundred and twenty patients with burn were randomly divided into control group and observation group( 60 cases each group). The control group and observation group were treated with Mabel burn cream and compound lithospermum ointment, respectively. Results: The cure rate in the observation group( 95. 0%) was higher than that in the control group( 80. 0%)( P 0. 05). The healing time of observation group with shallow Ⅱ wound( 9. 2 3. 1) d was shorter than that in control group( 15. 3 3. 2) d( P 0. 01). No obvious scar in the healing wound in two groups was found. The healing time of observation group with deep Ⅱ wound( 15. 3 3. 2) d was shorter than that in control group( 20 2. 6) d( P 0. 01). Conclusions: Compound lithospermum ointment in the treatment of middle and small small area burn is high cure rate and short healing time.
2013, 37(11): 1433-1435.
Abstract:
Objective: To explore the value of continuous fetal heart rate monitoring in labor for diagnosing fetal distress. Methods: One thousand two hundred and ninety-eight full-term pregnant women with single fetus and 862 pregnant women without continuous fetal heart rate monitoring in labor were set as the observation and control group,respectively. The fetal heart rate of the observation group was continuously monitored using central type monitor system in labor. The amniotic fluid turbidity rate,cesarean section rate with the indication of fetal distress,forceps delivery rate and neonatal asphyxia rate of two groups were calculated. The pH value of umbilical artery blood of neonates with abnormal fetal heart rate were measured. Results: The cesarean section rate with the indication of fetal distress of observation group was 21. 4%,which was significantly higher than that of control group( 15. 0%)( P 0. 01). The neonatal asphyxia rate and the severe asphyxia rate of observation group were 4. 5% and 1. 4%,which were significantly lower than those of control group( 7. 2% and 2. 7%)( P 0. 01 and P 0. 05). The fetal distress,amniotic fluid turbidity and forceps delivery with the indication of fetal distress rates of two groups had no significant difference( P 0. 05). The fetal distress rate of observation group was 10. 7% on the second stage of labor,which was lower than that of control group( 14. 0%)( P 0. 05). The pH value of umbilical artery blood of neonate with abnormal fetal heart rate pattern was 7. 25 0. 26,which was significantly lower than that of neonate with normal fetal heart rate pttern 7. 34 0. 18( P 0. 01). Conclusions: The continuous fetal heart rate monitoring using central type monitor system can early diagnose the fetal distress,which can provide the basis of early treatment and intervention.
Objective: To explore the value of continuous fetal heart rate monitoring in labor for diagnosing fetal distress. Methods: One thousand two hundred and ninety-eight full-term pregnant women with single fetus and 862 pregnant women without continuous fetal heart rate monitoring in labor were set as the observation and control group,respectively. The fetal heart rate of the observation group was continuously monitored using central type monitor system in labor. The amniotic fluid turbidity rate,cesarean section rate with the indication of fetal distress,forceps delivery rate and neonatal asphyxia rate of two groups were calculated. The pH value of umbilical artery blood of neonates with abnormal fetal heart rate were measured. Results: The cesarean section rate with the indication of fetal distress of observation group was 21. 4%,which was significantly higher than that of control group( 15. 0%)( P 0. 01). The neonatal asphyxia rate and the severe asphyxia rate of observation group were 4. 5% and 1. 4%,which were significantly lower than those of control group( 7. 2% and 2. 7%)( P 0. 01 and P 0. 05). The fetal distress,amniotic fluid turbidity and forceps delivery with the indication of fetal distress rates of two groups had no significant difference( P 0. 05). The fetal distress rate of observation group was 10. 7% on the second stage of labor,which was lower than that of control group( 14. 0%)( P 0. 05). The pH value of umbilical artery blood of neonate with abnormal fetal heart rate pattern was 7. 25 0. 26,which was significantly lower than that of neonate with normal fetal heart rate pttern 7. 34 0. 18( P 0. 01). Conclusions: The continuous fetal heart rate monitoring using central type monitor system can early diagnose the fetal distress,which can provide the basis of early treatment and intervention.
2013, 37(11): 1436-1437,1440.
Abstract:
Objective: To explore the curative effects of microwave endometrial ablation( MEA) in the treatment of abnormal uterine bleeding. Methods: The endometrium of 36 patients with abnormal uterine bleeding was removed by microwave therapy instrument. The curative effects of all patients were followed up. Results: All patients were followed up for 18 months. Amenorrhea in 18 cases, significant improvement in 12 cases,menstrual quantity decreasing in 3 cases,menses non-improvement in 3 cases were found,the total effective rate was 91. 7%( 33 /36). Among 36 patients,10 in 12 cases with obvious dysmenorrhea were improved after operation. The menstruation and haemoglobin quantity of all patients 18 months postoperation were less and higher than those before operation, respectively( P 0. 01). All patients were operated in the outpatient,the operation time was 160- 202 s,and there were no serious complications such as bleeding,infection and uterine perforation. Conclusions: MEA is an effective,safe and simple means in the treatment of abnormal uterine bleeding,especially for these patients with severe medical disease.
Objective: To explore the curative effects of microwave endometrial ablation( MEA) in the treatment of abnormal uterine bleeding. Methods: The endometrium of 36 patients with abnormal uterine bleeding was removed by microwave therapy instrument. The curative effects of all patients were followed up. Results: All patients were followed up for 18 months. Amenorrhea in 18 cases, significant improvement in 12 cases,menstrual quantity decreasing in 3 cases,menses non-improvement in 3 cases were found,the total effective rate was 91. 7%( 33 /36). Among 36 patients,10 in 12 cases with obvious dysmenorrhea were improved after operation. The menstruation and haemoglobin quantity of all patients 18 months postoperation were less and higher than those before operation, respectively( P 0. 01). All patients were operated in the outpatient,the operation time was 160- 202 s,and there were no serious complications such as bleeding,infection and uterine perforation. Conclusions: MEA is an effective,safe and simple means in the treatment of abnormal uterine bleeding,especially for these patients with severe medical disease.
2013, 37(11): 1438-1440.
Abstract:
Objective: To explore a rational therapy for recurrent esophageal carcinoma after radical radiotherapy. Methods: The clinical data of 21 patients with esophageal carcinoma having received esophagectomy were analyzed and compared with those of 22 patients treated by conservative therapy. Results: Twenty cases in the surgery group( 95. 2%) underwent esophagectomy. Postoperative hyoxemia occurred in 15 cases,anastomotic leakage in 1 case and pulmonary infection in 2 cases. The 1,3 and 5-year survival rate was 71. 4%,33. 3% and 14. 3% respectively in the surgery group,while the 1-year survival rate was 45. 5% in the conservative treatment group,the difference was not significant( P 0. 05),no one in the conservative treatment group survived after 2 years,and their survival duration was 2- 17 months. The satisfaction degree half-year after the recurrence was 66. 7% in the surgery group,which was significantly higher than the 27. 3% in the conservative treatment group( P 0. 01). Conclusions: Surgery is of great risk for patients with recurrent esophageal carcinoma after curative radiotherapy,so attentive peril-operation preparation is essential. Operation may bring survival benefit to those patients.
Objective: To explore a rational therapy for recurrent esophageal carcinoma after radical radiotherapy. Methods: The clinical data of 21 patients with esophageal carcinoma having received esophagectomy were analyzed and compared with those of 22 patients treated by conservative therapy. Results: Twenty cases in the surgery group( 95. 2%) underwent esophagectomy. Postoperative hyoxemia occurred in 15 cases,anastomotic leakage in 1 case and pulmonary infection in 2 cases. The 1,3 and 5-year survival rate was 71. 4%,33. 3% and 14. 3% respectively in the surgery group,while the 1-year survival rate was 45. 5% in the conservative treatment group,the difference was not significant( P 0. 05),no one in the conservative treatment group survived after 2 years,and their survival duration was 2- 17 months. The satisfaction degree half-year after the recurrence was 66. 7% in the surgery group,which was significantly higher than the 27. 3% in the conservative treatment group( P 0. 01). Conclusions: Surgery is of great risk for patients with recurrent esophageal carcinoma after curative radiotherapy,so attentive peril-operation preparation is essential. Operation may bring survival benefit to those patients.
2013, 37(11): 1441-1442.
Abstract:
Objective: To explore the relationship between neonatal pathologic jaundice and hearing impairment. Methods: Eighty-two neonates with pathologic jaundice were divided into mild-moderate jaundice group 50 cases( the bilirubin was 221 to 340 mol / L) and heavy jaundice group 32 cases( the bilirubin was higher than or equal to 340 mol / L) according to the level of bilirubin. Fourty neonates with physiologic jaundice were set as control group. Auditory evoked potentials( BAEP) of all neonates were measured within 1 week after birth. Results: The BAEP abnormality in single ear for 10 cases and two ears for 2 cases in mild-moderate jaundice group were found,the abnormal rate of BAEP was 24. 0%. The BAEP abnormality in single ear for 8 cases and two ears for 4 cases in heavy jaundice group were found,the abnormal rate of BAEP was 37. 5%. The BAEP abnormality in single ear for 2 cases in control group were found,the abnormal rate of BAEP was 5. 0%. The differences of BAEP abnormal rate in three groups had statistical significance( P 0. 01). Conclusions: The hearing of neonate with pathologic jaundice is easy to be harmed. The incidence and degree of hearing impairment are higher and heavy with the serum total bilirubin level increasing and the duration lengthening.
Objective: To explore the relationship between neonatal pathologic jaundice and hearing impairment. Methods: Eighty-two neonates with pathologic jaundice were divided into mild-moderate jaundice group 50 cases( the bilirubin was 221 to 340 mol / L) and heavy jaundice group 32 cases( the bilirubin was higher than or equal to 340 mol / L) according to the level of bilirubin. Fourty neonates with physiologic jaundice were set as control group. Auditory evoked potentials( BAEP) of all neonates were measured within 1 week after birth. Results: The BAEP abnormality in single ear for 10 cases and two ears for 2 cases in mild-moderate jaundice group were found,the abnormal rate of BAEP was 24. 0%. The BAEP abnormality in single ear for 8 cases and two ears for 4 cases in heavy jaundice group were found,the abnormal rate of BAEP was 37. 5%. The BAEP abnormality in single ear for 2 cases in control group were found,the abnormal rate of BAEP was 5. 0%. The differences of BAEP abnormal rate in three groups had statistical significance( P 0. 01). Conclusions: The hearing of neonate with pathologic jaundice is easy to be harmed. The incidence and degree of hearing impairment are higher and heavy with the serum total bilirubin level increasing and the duration lengthening.
2013, 37(11): 1443-1445.
Abstract:
Objective: To determine the correlation of serum neuroglobin( NGB) level in neonatal hypoxic-ischemic encephalopathy( HIE) and 20 neonatal behavioral neurological assessment( NBNA),which offers a fundamental prevention of cerebral hypoxia and cerebral ischemia. Methods: Forty-four cases of HIE were selected,including mild in 22 cases,moderate in 14 cases,and severe in 8 cases. Ten full-term newborns were selected as the normal control group. The serum was collected at 3 h,3 d,7 d after birth,and the serum NGB level was determined by enzyme-linked immunosorbent assay method. The 20 NBNA score was determined 1 d,7 d,14 d after birth. The correlation analysis between the serum NGB level and NBNA score was done,The correlations were also compared among acute and convalescent phases in HIE group and control group. Results: In HIE group,the serum NGB level in acute phase of moderate and severe groups was significantly higher than in mild HIE group and control group( P 0. 01). Serum NGB level was declined in convalescent phase of moderate and severe groups,but still higher than in control group( P 0. 01). The serum NGB level was negatively correlated with NBNA score( P 0. 01). Conclusions: Serum NGB level in the acute and convalescent phases was negatively correlated with NBNA score. There is an important clinical significance of serum NGB level as an early evaluation of HIE severity of brain injury and recovery target.
Objective: To determine the correlation of serum neuroglobin( NGB) level in neonatal hypoxic-ischemic encephalopathy( HIE) and 20 neonatal behavioral neurological assessment( NBNA),which offers a fundamental prevention of cerebral hypoxia and cerebral ischemia. Methods: Forty-four cases of HIE were selected,including mild in 22 cases,moderate in 14 cases,and severe in 8 cases. Ten full-term newborns were selected as the normal control group. The serum was collected at 3 h,3 d,7 d after birth,and the serum NGB level was determined by enzyme-linked immunosorbent assay method. The 20 NBNA score was determined 1 d,7 d,14 d after birth. The correlation analysis between the serum NGB level and NBNA score was done,The correlations were also compared among acute and convalescent phases in HIE group and control group. Results: In HIE group,the serum NGB level in acute phase of moderate and severe groups was significantly higher than in mild HIE group and control group( P 0. 01). Serum NGB level was declined in convalescent phase of moderate and severe groups,but still higher than in control group( P 0. 01). The serum NGB level was negatively correlated with NBNA score( P 0. 01). Conclusions: Serum NGB level in the acute and convalescent phases was negatively correlated with NBNA score. There is an important clinical significance of serum NGB level as an early evaluation of HIE severity of brain injury and recovery target.
2013, 37(11): 1446-1448.
Abstract:
Objective: To study the therapeutic effect of Yiqihuoxue decoctionin in treatment of carotid arteriosclerosis plaque in diabetic patients. Methods: Sixty cases of diabetes with carotid arteriosclerosis plaque were randomly divided into treatment group and control group,30 cases in each. Both groups received the routine western medicine therapy,and the treatment group were given yiqihuoxue decoction orally twice a day,200 ml each time in addition,8 weeks as a circle. The plaque regression rate,the change of haemorheology and the blood glucose were recorded. Results: The plaque regression rate of the treatment group was significantly higher than that of the control( P 0. 01); and the change of haemorheology improved obviously in the treatment group( P 0. 01). Conclusions: Yiqihuoxue decoction is effective in treatment of the carotid arteriosclerosis plaque in diabetics.
Objective: To study the therapeutic effect of Yiqihuoxue decoctionin in treatment of carotid arteriosclerosis plaque in diabetic patients. Methods: Sixty cases of diabetes with carotid arteriosclerosis plaque were randomly divided into treatment group and control group,30 cases in each. Both groups received the routine western medicine therapy,and the treatment group were given yiqihuoxue decoction orally twice a day,200 ml each time in addition,8 weeks as a circle. The plaque regression rate,the change of haemorheology and the blood glucose were recorded. Results: The plaque regression rate of the treatment group was significantly higher than that of the control( P 0. 01); and the change of haemorheology improved obviously in the treatment group( P 0. 01). Conclusions: Yiqihuoxue decoction is effective in treatment of the carotid arteriosclerosis plaque in diabetics.
2013, 37(11): 1450-1452.
Abstract:
Objective: To observe the changes of blood gas level and myocardial enzymology in children with severe pneumonia. Methods: The changes of the electrocardiogram,blood gas level and myocardial enzymology in 58 children with severe pneumonia were analyzed retrospectively; and the correlations among them were studied. Results: The level of blood gas analysis in the group with abnormal myocardial enzymology was obviously higher than that with normal myocardial enzymology( P 0. 01); There was a positive correlation between the level of blood gas analysis(-BE) and myocardial enzymology( CK-MB) in the 32 cases with abnormal myocardial enzymology( P 0. 01). Among the 58 cases of severe pneumonia,51 presented great changes in electrocardiogram,32 of whom were in the group with abnormal myocardial enzymology( 62. 75%); 11 cases were accompanied by heart failure( 18. 97%). Conclusions: Hypoxemia and metabolic acidosis facilitate the genesis and development of myocardial damage. Early intervention could prevent the incidence of heart failure. Blood gas analysis,myocardial enzymology detection and electrocardiogram examination are beneficial to the early diagnosis and prognosis of myocardial damage in children with severe pneumonia.
Objective: To observe the changes of blood gas level and myocardial enzymology in children with severe pneumonia. Methods: The changes of the electrocardiogram,blood gas level and myocardial enzymology in 58 children with severe pneumonia were analyzed retrospectively; and the correlations among them were studied. Results: The level of blood gas analysis in the group with abnormal myocardial enzymology was obviously higher than that with normal myocardial enzymology( P 0. 01); There was a positive correlation between the level of blood gas analysis(-BE) and myocardial enzymology( CK-MB) in the 32 cases with abnormal myocardial enzymology( P 0. 01). Among the 58 cases of severe pneumonia,51 presented great changes in electrocardiogram,32 of whom were in the group with abnormal myocardial enzymology( 62. 75%); 11 cases were accompanied by heart failure( 18. 97%). Conclusions: Hypoxemia and metabolic acidosis facilitate the genesis and development of myocardial damage. Early intervention could prevent the incidence of heart failure. Blood gas analysis,myocardial enzymology detection and electrocardiogram examination are beneficial to the early diagnosis and prognosis of myocardial damage in children with severe pneumonia.
2013, 37(11): 1454-1456.
Abstract:
Objective: To compared the influence of bipolar coagulation and conventional stripping method on patients before and after tonsillectomy. Methods: Eighty patients were divided into bipolar electrocoagulation group 43 cases and control group 37 cases. The control group were performed conventional stripping method to dissect the tonsil,while the bipolar electrocoagulation group were treated by bipolar electrocoagulation. The intraoperative bleeding volume,operation time,postoperative pain,the time to resume normal activities and normal diet,and the shedding of pseudomembrane were recorded in the two groups. Results: The operation time and bleeding volume of the bipolar coagulation group were less than those of the control group; the bipolar coagulation group took less time to resume normal diet and suffered less postoperative pain than the control group,but took longer time to shed the pseudomembrane. The difference was statistically significant( P 0. 01). Conclusions: The bipolar electrocoagulation is superior to the conventional dissection method in tonsillectomy. It has the advantages of less bleeding,shorter operation time,less postoperative pain and earlier resumption of diet. The method is easy to grasp and worth popularizing.
Objective: To compared the influence of bipolar coagulation and conventional stripping method on patients before and after tonsillectomy. Methods: Eighty patients were divided into bipolar electrocoagulation group 43 cases and control group 37 cases. The control group were performed conventional stripping method to dissect the tonsil,while the bipolar electrocoagulation group were treated by bipolar electrocoagulation. The intraoperative bleeding volume,operation time,postoperative pain,the time to resume normal activities and normal diet,and the shedding of pseudomembrane were recorded in the two groups. Results: The operation time and bleeding volume of the bipolar coagulation group were less than those of the control group; the bipolar coagulation group took less time to resume normal diet and suffered less postoperative pain than the control group,but took longer time to shed the pseudomembrane. The difference was statistically significant( P 0. 01). Conclusions: The bipolar electrocoagulation is superior to the conventional dissection method in tonsillectomy. It has the advantages of less bleeding,shorter operation time,less postoperative pain and earlier resumption of diet. The method is easy to grasp and worth popularizing.
2013, 37(11): 1461-1465.
Abstract:
Objective: To build up the comprehensive intervention model guided by the theory of health promotion and based on college for undergraduate internet addiction disorder( IAD) so as to evaluate its effects on undergraduate IAD,psychological health state and dealing measures. Methods:Two hundred and sixteen and 200 college students randomly selected from School A and B were divided into the intervention group and control group,respectively. The comprehensive intervention measures including health education lecture,community activities,social practice,peer education and individual teaching were implemented for a year in intervention group, the control group were not given any intervention measures. The internet addiction condition,psychological health status and dealing measures in two groups were compared before and after intervention. Results: The occurrence rates of internet addiction tendency and IAD in intervention group after the intervention were 4. 63% and 3. 24%,respectively,compared with before intervention,the differences of which were statistical significance( P 0. 01). Compared with before intervention,the students with time online less than 2 h and more than 4 h in intervention group after the intervention were significantly increased and decreased,respectively( P 0. 01), the differences in two groups were statistical significance( P 0. 01). The scores of SCL-90 and all factors and negative dealing measures in intervention group after intervention were significantly lowered than those in intervention group before intervention and control group( P 0. 01),the score of positive dealing measures in intervention group after intervention was significantly higher than that in control group( P 0. 01). Conclusions: The comprehensive intervention model guided by the theory of health promotion and based on college for undergraduate IAD can effectively reduce the occurrence rates of internet addiction tendency and IAD,improve the internet addiction symptoms and cognition of using internet,increase the psychological health level and improve the negative dealing measures in college students.
Objective: To build up the comprehensive intervention model guided by the theory of health promotion and based on college for undergraduate internet addiction disorder( IAD) so as to evaluate its effects on undergraduate IAD,psychological health state and dealing measures. Methods:Two hundred and sixteen and 200 college students randomly selected from School A and B were divided into the intervention group and control group,respectively. The comprehensive intervention measures including health education lecture,community activities,social practice,peer education and individual teaching were implemented for a year in intervention group, the control group were not given any intervention measures. The internet addiction condition,psychological health status and dealing measures in two groups were compared before and after intervention. Results: The occurrence rates of internet addiction tendency and IAD in intervention group after the intervention were 4. 63% and 3. 24%,respectively,compared with before intervention,the differences of which were statistical significance( P 0. 01). Compared with before intervention,the students with time online less than 2 h and more than 4 h in intervention group after the intervention were significantly increased and decreased,respectively( P 0. 01), the differences in two groups were statistical significance( P 0. 01). The scores of SCL-90 and all factors and negative dealing measures in intervention group after intervention were significantly lowered than those in intervention group before intervention and control group( P 0. 01),the score of positive dealing measures in intervention group after intervention was significantly higher than that in control group( P 0. 01). Conclusions: The comprehensive intervention model guided by the theory of health promotion and based on college for undergraduate IAD can effectively reduce the occurrence rates of internet addiction tendency and IAD,improve the internet addiction symptoms and cognition of using internet,increase the psychological health level and improve the negative dealing measures in college students.
2013, 37(11): 1466-1470.
Abstract:
Objective: To evaluate the value of full-field digital mammography( FFDM) and preoperative localization in the diagosis of nonpalpable breast lesions( NPBL). Methods: NPBL in 147 patients were located by FFDM,and confirmed by postoperative pathological results. Results: One hundred and fifty-one lesions were found in 147 patients,149 and 2 lesions were successfully located at first time and the second,respectively. The satisfaction rate was 98. 6%. One hundreed and forty-six lesions were successfully resected. Breast carcinoma in 40 lesions and benign tumor in 111 lesions were found. The accuracy,sensitivity,specificity,positive predictive value and negative predictive value of NPBL in FFDM and location were 72. 2%,90. 0%,65. 8%,48. 6% and 94. 8%,respectively. The differences of shape,distribution and border of calcification and shape,border and density of nodules in the diagnosis of benign and malignant tumor had statistical significance( P 0. 05 to P 0. 01). Conclusions: The NPBL localization guided by FFDM is accurate,less trauma and simple,which can guide the clinic.
Objective: To evaluate the value of full-field digital mammography( FFDM) and preoperative localization in the diagosis of nonpalpable breast lesions( NPBL). Methods: NPBL in 147 patients were located by FFDM,and confirmed by postoperative pathological results. Results: One hundred and fifty-one lesions were found in 147 patients,149 and 2 lesions were successfully located at first time and the second,respectively. The satisfaction rate was 98. 6%. One hundreed and forty-six lesions were successfully resected. Breast carcinoma in 40 lesions and benign tumor in 111 lesions were found. The accuracy,sensitivity,specificity,positive predictive value and negative predictive value of NPBL in FFDM and location were 72. 2%,90. 0%,65. 8%,48. 6% and 94. 8%,respectively. The differences of shape,distribution and border of calcification and shape,border and density of nodules in the diagnosis of benign and malignant tumor had statistical significance( P 0. 05 to P 0. 01). Conclusions: The NPBL localization guided by FFDM is accurate,less trauma and simple,which can guide the clinic.
2013, 37(11): 1471-1472.
Abstract:
Objective: To investigate the value of B-ultrasound and molybdenum X-ray in the diagnosis of breast nodules. Methods: Eighty patients with breast space occupying were detected with B-ultrasound and molybdenum X-ray examination. The diagnostic results and the clinical and pathological findings were compared to confirm the breast tumor. Results: The coincidence rate of B-ultrasound diagnosis and clinical and pathological conditions was 90%. The coincidence rate of B-ultrasound in the diagnosis of fibroadenoma was higher than that in molybdenum X-ray( P 0. 05). The coincidence rate differences of B-ultrasound and molybdenum X-ray in the diagnosis of breast cyst,mammary gland hyperplasia and breast cancer had no statistical significance( P 0. 05). Conclusions: Bultrasound is high accuracy,no radiation damage and lower price,which has an important value in the dignosis of breast neoplasms.
Objective: To investigate the value of B-ultrasound and molybdenum X-ray in the diagnosis of breast nodules. Methods: Eighty patients with breast space occupying were detected with B-ultrasound and molybdenum X-ray examination. The diagnostic results and the clinical and pathological findings were compared to confirm the breast tumor. Results: The coincidence rate of B-ultrasound diagnosis and clinical and pathological conditions was 90%. The coincidence rate of B-ultrasound in the diagnosis of fibroadenoma was higher than that in molybdenum X-ray( P 0. 05). The coincidence rate differences of B-ultrasound and molybdenum X-ray in the diagnosis of breast cyst,mammary gland hyperplasia and breast cancer had no statistical significance( P 0. 05). Conclusions: Bultrasound is high accuracy,no radiation damage and lower price,which has an important value in the dignosis of breast neoplasms.
2013, 37(11): 1473-1475.
Abstract:
Objective: To investigate the application value of virtual touch tissue quantification( VTQ) technique in liver damage detection of hepatolenticular dageneration( HD) patient. Methods: One hundred fourty-six HD patients were detected using ultrasound and VTQ technique,their liver ultrasonographic manifestations were classified,and the VTQ value of two kinds of liver sonograms were compared. Results: The VTQ value of 146 HD patients was 1. 14 to 3. 71 m / s. The difference of VTQ value in two kinds of liver sonograms had statistic significance( P 0. 01). The VTQ value of light liver disease HD patients with fatty infiltration type and glinting echogemic dots type sonogram were lower than those in patients with the tree like type,rock stratum type and nodular type sonograms( P 0. 01). The VTQ value of patients with nodular type sonogram was higher than that in patients with tree like type and rock stratum type sonograms( P 0. 01),the VTQ value difference between tree like type and rock stratum type patients has no statistic significance( P 0. 05). Conclusions: VTQ technique can objectively evaluate the liver damage degree of HD patient.
Objective: To investigate the application value of virtual touch tissue quantification( VTQ) technique in liver damage detection of hepatolenticular dageneration( HD) patient. Methods: One hundred fourty-six HD patients were detected using ultrasound and VTQ technique,their liver ultrasonographic manifestations were classified,and the VTQ value of two kinds of liver sonograms were compared. Results: The VTQ value of 146 HD patients was 1. 14 to 3. 71 m / s. The difference of VTQ value in two kinds of liver sonograms had statistic significance( P 0. 01). The VTQ value of light liver disease HD patients with fatty infiltration type and glinting echogemic dots type sonogram were lower than those in patients with the tree like type,rock stratum type and nodular type sonograms( P 0. 01). The VTQ value of patients with nodular type sonogram was higher than that in patients with tree like type and rock stratum type sonograms( P 0. 01),the VTQ value difference between tree like type and rock stratum type patients has no statistic significance( P 0. 05). Conclusions: VTQ technique can objectively evaluate the liver damage degree of HD patient.
2013, 37(11): 1475-1476.
Abstract:
Objective: To investigate the type distribution of human papillomavirus( HPV) in patients with condyloma acuminatum( CA). Methods: The HPV DNA( high-risk and low-risk type) of lesion and secretion of condyloma acuminatum in CA patients were detected by fluorescent quantitative polymerase chain reaction. Results: The positive detection rates of HPV in 110 CA patients were 100%. Low-risk,high-risk and high and low-risk compound infection in all CA patients were 64. 55%,14. 54% and 20. 91%,respectively. The detection rate differences of HPV in different gender and age had no statistical significance( P 0. 05). Conclusions: The low-risk HPV infection is mostly and high-risk HPV infection is relatively high in CA patients. It suggests that the prevention and treatment of genital tumor should be strengthened.
Objective: To investigate the type distribution of human papillomavirus( HPV) in patients with condyloma acuminatum( CA). Methods: The HPV DNA( high-risk and low-risk type) of lesion and secretion of condyloma acuminatum in CA patients were detected by fluorescent quantitative polymerase chain reaction. Results: The positive detection rates of HPV in 110 CA patients were 100%. Low-risk,high-risk and high and low-risk compound infection in all CA patients were 64. 55%,14. 54% and 20. 91%,respectively. The detection rate differences of HPV in different gender and age had no statistical significance( P 0. 05). Conclusions: The low-risk HPV infection is mostly and high-risk HPV infection is relatively high in CA patients. It suggests that the prevention and treatment of genital tumor should be strengthened.
2013, 37(11): 1477-1479.
Abstract:
Objective: To explore the value of the prostate-specific antigen( tPSA) and its related index in the diagnosis of prostate cancer( PCa). Methods: Forty-five patients with PCa,50 patients with benign prostatic hyperplasia and 45 healthy volunteers were served as observation group,benign prostate hyperplasia( BPH) group and control group,respectively. The levels of serum tPSA and free tPSA( fPSA) were measured by enzyme amplification chemiluminescence analyzer,and the ratio of fPSA and tPSA was calculated. Results: The levels of tPSA and fPSA in observation group were significantly higher than those in BPH group( P 0. 01),the levels of tPSA and fPSA in BPH group were significantly higher than those in control group( P 0. 01). When the value of tPSA were less than 4. 0,4. 0 to 10. 0 and greater than 10. 0 g / L,BPH patients accounted for 64. 0%,30. 0% and 4. 0%,respectively,PCa patients accounted for 8. 9%,22. 2% and 68. 9%,respectively,and the healthy people accounted for 97. 8%,2. 2% and 0. 0%,respectively, the differences of there groups have statistical significance( P 0. 01). The ratio of fPSA and tPSA in observation group was significantly lower than that in control group and BPH group( P 0. 01),the ratio of fPSA and tPSA in BPH group was significantly lower than that in control group( P 0. 01). Within the 4. 0 to 10. 0 of gray area,the critical value of fPSA and tPSA in observation group and BPH group was 0. 16,the ratio of fPSA and tPSA in 80. 0% of patients of observation group was less than 0. 16,and the ratio of fPSA and tPSA in 86. 7% of patients in BHP group was greater than 0. 16,the difference of which was statistical significance( P = 0. 002). Conclusions: The tPSA combined with the ratio of fPSA /tPSA can be used as an important basis in the diagnosis of prostate cancer.
Objective: To explore the value of the prostate-specific antigen( tPSA) and its related index in the diagnosis of prostate cancer( PCa). Methods: Forty-five patients with PCa,50 patients with benign prostatic hyperplasia and 45 healthy volunteers were served as observation group,benign prostate hyperplasia( BPH) group and control group,respectively. The levels of serum tPSA and free tPSA( fPSA) were measured by enzyme amplification chemiluminescence analyzer,and the ratio of fPSA and tPSA was calculated. Results: The levels of tPSA and fPSA in observation group were significantly higher than those in BPH group( P 0. 01),the levels of tPSA and fPSA in BPH group were significantly higher than those in control group( P 0. 01). When the value of tPSA were less than 4. 0,4. 0 to 10. 0 and greater than 10. 0 g / L,BPH patients accounted for 64. 0%,30. 0% and 4. 0%,respectively,PCa patients accounted for 8. 9%,22. 2% and 68. 9%,respectively,and the healthy people accounted for 97. 8%,2. 2% and 0. 0%,respectively, the differences of there groups have statistical significance( P 0. 01). The ratio of fPSA and tPSA in observation group was significantly lower than that in control group and BPH group( P 0. 01),the ratio of fPSA and tPSA in BPH group was significantly lower than that in control group( P 0. 01). Within the 4. 0 to 10. 0 of gray area,the critical value of fPSA and tPSA in observation group and BPH group was 0. 16,the ratio of fPSA and tPSA in 80. 0% of patients of observation group was less than 0. 16,and the ratio of fPSA and tPSA in 86. 7% of patients in BHP group was greater than 0. 16,the difference of which was statistical significance( P = 0. 002). Conclusions: The tPSA combined with the ratio of fPSA /tPSA can be used as an important basis in the diagnosis of prostate cancer.
2013, 37(11): 1480-1482.
Abstract:
Objective: To investigate the prevalence of functional constipation( FC) in Minhang District of Shanghai. Methods: A questionnaire survey was conducted among permanent inhabitants more than 18 years old in Minhang District of Shanghai by randomizing clustered sampling. All the questionnaires were filled out by trained doctors. Results: From May to August 2010,there were 2 113 inhabitants received investigation,in which there were 139 FC patients. The prevalence rate of FC is 6. 6% according to Rome Ⅲ criteria. The risk factors maybe include female,elder,employee in government,higher incomes,insomnolence( P 0. 05 to P 0. 01). Conclusions: FC is a common disorder in Minhang District of Shanghai,and is influenced by many factors.
Objective: To investigate the prevalence of functional constipation( FC) in Minhang District of Shanghai. Methods: A questionnaire survey was conducted among permanent inhabitants more than 18 years old in Minhang District of Shanghai by randomizing clustered sampling. All the questionnaires were filled out by trained doctors. Results: From May to August 2010,there were 2 113 inhabitants received investigation,in which there were 139 FC patients. The prevalence rate of FC is 6. 6% according to Rome Ⅲ criteria. The risk factors maybe include female,elder,employee in government,higher incomes,insomnolence( P 0. 05 to P 0. 01). Conclusions: FC is a common disorder in Minhang District of Shanghai,and is influenced by many factors.
2013, 37(11): 1483-1484.
Abstract:
Objective: To know the prevalence of Helicobacter pylori( Hp) infection in hospital staffs. Methods: The serum anti-Hp IgG of 1 582 hospital staffs and 213 non-hospital staffs were determined using ELISA. Results: The Hp antibody positive rate in hospital staffs was significantly higher than that in non- hospital staffs( P 0. 01),the Hp antibody positive rate in patients with indigestion symptoms was significantly higher than that in patients without indigestion symptoms( P 0. 01). Conclusions: Hospital staff is the high-risk groups of Hp infection.
Objective: To know the prevalence of Helicobacter pylori( Hp) infection in hospital staffs. Methods: The serum anti-Hp IgG of 1 582 hospital staffs and 213 non-hospital staffs were determined using ELISA. Results: The Hp antibody positive rate in hospital staffs was significantly higher than that in non- hospital staffs( P 0. 01),the Hp antibody positive rate in patients with indigestion symptoms was significantly higher than that in patients without indigestion symptoms( P 0. 01). Conclusions: Hospital staff is the high-risk groups of Hp infection.
2013, 37(11): 1485-1487.
Abstract:
Objective: To explore the relationship between the deficiency of folic acid during gestational period and gravida megaloblastic anemia and fetus neural tube defect. Methods: One thousand and forty-one gravidas from out-patients clinic for regular prenatal care were investigated,whose first identification were from 12 to 14 weeks of pregnancy. All gravidas were divided into two groups( A and B group) according to oral folic acid administrate or not 3 months befor and after pregnancy. Group A( 614 cases) took folic acid by oral from 3 months( at least 1 month) before pregnancy to 3 months after pregnancy. Group B( 427 cases) did not take folic acid by oral. Blood analysis,the serum content of folic acid and B ultrasound of two groups were investigated to compare the occurrence of gravida megaloblastic anemia and fetus neural tube defect. Results: The levels of red cells and serum folic acid of group A were significantly higher than that in group B( P 0. 01). The incidence of megaloblastic anemia of gravida taking folic acid by oral in 3 months( at least 1 month) before pregnancy was obviously lower than that in B group( P 0. 01). Compared with B group,the incidence of fetus neural tube defect of A group was decreased. Conclusions: Gravida who takes folic acid by oral 3 months befor and after pregnancy can reduce the incidence of gravida megaloblastic anemia and fetus neural tube defect.
Objective: To explore the relationship between the deficiency of folic acid during gestational period and gravida megaloblastic anemia and fetus neural tube defect. Methods: One thousand and forty-one gravidas from out-patients clinic for regular prenatal care were investigated,whose first identification were from 12 to 14 weeks of pregnancy. All gravidas were divided into two groups( A and B group) according to oral folic acid administrate or not 3 months befor and after pregnancy. Group A( 614 cases) took folic acid by oral from 3 months( at least 1 month) before pregnancy to 3 months after pregnancy. Group B( 427 cases) did not take folic acid by oral. Blood analysis,the serum content of folic acid and B ultrasound of two groups were investigated to compare the occurrence of gravida megaloblastic anemia and fetus neural tube defect. Results: The levels of red cells and serum folic acid of group A were significantly higher than that in group B( P 0. 01). The incidence of megaloblastic anemia of gravida taking folic acid by oral in 3 months( at least 1 month) before pregnancy was obviously lower than that in B group( P 0. 01). Compared with B group,the incidence of fetus neural tube defect of A group was decreased. Conclusions: Gravida who takes folic acid by oral 3 months befor and after pregnancy can reduce the incidence of gravida megaloblastic anemia and fetus neural tube defect.
2013, 37(11): 1489-1492.
Abstract:
Objective: To investigate the existing deficiency in professional master degree of clinical medicine postgraduate training of 3 medical colleges and universities in Anhui province and the related countermeasures. Methods: The method of questionnaire survey was adopted to investigate the situations of the professional degree graduate training of 3 medical colleges and universities for 123 clinical medicine masters in Anhui province,and the investigation results were analyzed accordingly. Results: New college students accounted for 81. 30% of clinical medicine graduate in professional master degree,and the former graduates accounted for 18. 70%. In scientific research and clinical skills,39. 84% of respondents only make the literature reviews,52. 85% of respondents can not manage the beds independently and 33. 33% of respondents seldom or occasionally conduct any independent medical operation. Only 12. 19% of respondents told that there was the performance evaluation mechanism after finished cycling all sections. Conclusions: Some problems still exist in training mode,clinical skill training and the performance appraisal system of professional master degree of clinical medicine postgraduate training. Such measures as making a reasonable scientific research plan,strengthening the standard management of post graduate training department and improving the theoretical knowledge and clinical skills examination system can further improve the quality of professional master degree of clinical medicine postgraduate training.
Objective: To investigate the existing deficiency in professional master degree of clinical medicine postgraduate training of 3 medical colleges and universities in Anhui province and the related countermeasures. Methods: The method of questionnaire survey was adopted to investigate the situations of the professional degree graduate training of 3 medical colleges and universities for 123 clinical medicine masters in Anhui province,and the investigation results were analyzed accordingly. Results: New college students accounted for 81. 30% of clinical medicine graduate in professional master degree,and the former graduates accounted for 18. 70%. In scientific research and clinical skills,39. 84% of respondents only make the literature reviews,52. 85% of respondents can not manage the beds independently and 33. 33% of respondents seldom or occasionally conduct any independent medical operation. Only 12. 19% of respondents told that there was the performance evaluation mechanism after finished cycling all sections. Conclusions: Some problems still exist in training mode,clinical skill training and the performance appraisal system of professional master degree of clinical medicine postgraduate training. Such measures as making a reasonable scientific research plan,strengthening the standard management of post graduate training department and improving the theoretical knowledge and clinical skills examination system can further improve the quality of professional master degree of clinical medicine postgraduate training.
2013, 37(11): 1493-1496.
Abstract:
Objective: To explore the teaching model of project-based learning( PBL) in Medical English teaching so as to improve the classroom teaching effects. Methods: Based on the theory of PBL,this paper put forwards seven steps including setting goals,classroom instruction,designing project,selecting project,implementing project; presenting achievements and evaluating project to implement PBL in Medical English teaching. Results: The teaching quality of Medical English was improved and the student-centeredinstructional model was achieved by understanding the connotation and the implementing steps of PBL. Conclusions: The teaching model of PBL plays a positive role in consolidating language proficiency of medical students,improving their research competence and developing their emotion accomplishment.
Objective: To explore the teaching model of project-based learning( PBL) in Medical English teaching so as to improve the classroom teaching effects. Methods: Based on the theory of PBL,this paper put forwards seven steps including setting goals,classroom instruction,designing project,selecting project,implementing project; presenting achievements and evaluating project to implement PBL in Medical English teaching. Results: The teaching quality of Medical English was improved and the student-centeredinstructional model was achieved by understanding the connotation and the implementing steps of PBL. Conclusions: The teaching model of PBL plays a positive role in consolidating language proficiency of medical students,improving their research competence and developing their emotion accomplishment.
2013, 37(11): 1496-1498.
Abstract:
Objective: To reform the professional course teaching of preventive medicine undergraduate students for meeting the need of public health service development. Methods: Forty grade 3rd undergraduate students from preventive medicine were selected by stratified sampling method according to average scores,and divided into participatory and control group( 20 students in each group). The control group were taught by routine mode. Bilingual teaching was applied in participatory group,participatory forms included playing sketch,drawing,designing game and making model. Professional knowledge,attitude and behavior of two groups were compared. Results: The score difference of professional knowledge between two groups has no statistical significance( P 0. 05). The scores of attitude and behavior in participatory group were significantly higher than those in control group( P 0. 01). The teaching and social effects of participatory group were better than those in control group. Conclusions: Participatory teaching method can enrich practical education and improve the learning activity of professional knowledge,which can provide a model for related educators.
Objective: To reform the professional course teaching of preventive medicine undergraduate students for meeting the need of public health service development. Methods: Forty grade 3rd undergraduate students from preventive medicine were selected by stratified sampling method according to average scores,and divided into participatory and control group( 20 students in each group). The control group were taught by routine mode. Bilingual teaching was applied in participatory group,participatory forms included playing sketch,drawing,designing game and making model. Professional knowledge,attitude and behavior of two groups were compared. Results: The score difference of professional knowledge between two groups has no statistical significance( P 0. 05). The scores of attitude and behavior in participatory group were significantly higher than those in control group( P 0. 01). The teaching and social effects of participatory group were better than those in control group. Conclusions: Participatory teaching method can enrich practical education and improve the learning activity of professional knowledge,which can provide a model for related educators.
2013, 37(11): 1499-1500,1505.
Abstract:
Objective: To develop the best advantages of blended learning,contruct the medical genetics online course and combine make the network course and the traditional classroom teaching an organic unity. Methods: The medical genetics online course was constructed by useful software. Results: Medical genetics online course based on blended learning was achieved. Conclusions: The teaching environment and conditions of universities were greatly changed by information technology,medical genetics online course based on blended learning can combine the traditional classroom teaching and online learning into a unity,which can run fully the teaching characteristics and advantages.
Objective: To develop the best advantages of blended learning,contruct the medical genetics online course and combine make the network course and the traditional classroom teaching an organic unity. Methods: The medical genetics online course was constructed by useful software. Results: Medical genetics online course based on blended learning was achieved. Conclusions: The teaching environment and conditions of universities were greatly changed by information technology,medical genetics online course based on blended learning can combine the traditional classroom teaching and online learning into a unity,which can run fully the teaching characteristics and advantages.
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