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2013 Vol. 38, No. 6
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2013, 37(6): 645-647,651.
Abstract:
Objective:To investigate the relative contributions of dendritic cell(DC)in the development of protective immunity to Listeria monocytogenes(LM).Methods:DC subpopulation and phenotype characteristics during early infection of LM were examined.The BALB/c mice were infected by intravenously injection of 2 105LM.Spleen cell suspension was prepared from the mice sacrificed on days 3,5,7 post infection(p.i.).Diluted homogenates in sterile PBS were plated on LB plates to quantify bacterial load in spleen.The percentages of DC subsets,as well as surface molecule CD80 or CD86 expressed on spleen DC were determined by flow cytometry. Results:The overall number of CD4 -CD8+ 、CD4+CD8- and CD4-CD8- DC in the spleen increased significantly after infection with LM.The increases were approximate 1.5-fold augment in the CD4+DC by day 7,and an approximate 3-fold augment in the CD4-CD8+ and CD4-CD8- DC by day 5.The expression of CD80 and CD86 were significantly increased on days 3-5 p.i.and reached its peak on day 3 p.i.for BALB/c mice.Conclusions:These data provide new insight into the relative abundance and function of distinct CD11c-expressing populations during the early stage of LM infection.
Objective:To investigate the relative contributions of dendritic cell(DC)in the development of protective immunity to Listeria monocytogenes(LM).Methods:DC subpopulation and phenotype characteristics during early infection of LM were examined.The BALB/c mice were infected by intravenously injection of 2 105LM.Spleen cell suspension was prepared from the mice sacrificed on days 3,5,7 post infection(p.i.).Diluted homogenates in sterile PBS were plated on LB plates to quantify bacterial load in spleen.The percentages of DC subsets,as well as surface molecule CD80 or CD86 expressed on spleen DC were determined by flow cytometry. Results:The overall number of CD4 -CD8+ 、CD4+CD8- and CD4-CD8- DC in the spleen increased significantly after infection with LM.The increases were approximate 1.5-fold augment in the CD4+DC by day 7,and an approximate 3-fold augment in the CD4-CD8+ and CD4-CD8- DC by day 5.The expression of CD80 and CD86 were significantly increased on days 3-5 p.i.and reached its peak on day 3 p.i.for BALB/c mice.Conclusions:These data provide new insight into the relative abundance and function of distinct CD11c-expressing populations during the early stage of LM infection.
2013, 37(6): 648-651.
Abstract:
Objective:To investigate the effects of umbilical cord mesenchymal stem cells(MSCs)on hemopoietic function of bone marrow in mice with aplastic anemia(AA).Methods:Umbilical cord MSCs were primarily cultured with adherent method.All mice were divided into the model group,control group and MSCs infusion group.The aplastic anemia model mice established by immune-mediated means were infused with MSCs through caudalis vena.The peripheral hemogram and bone marrow morphology were observed at 3,7,14,21 and 28 days.The level of hematopoiesis negative regulatory factor(IFN-)was detected by ELISA.The colony-forming unit counts of bone marrow hemopoietic progenitor cells were measured.Results:The spindle-shaped MSCs extended from the tissue edge at 8-10 days after primary adherent culture of umbilical cord,and those were parallelly or whirlpool-shape growth with the cultivation.The increasing peripheral hemogram and decreasing IFN- levels of AA mice treated with MSCs were significant(P0.01).The colony forming units-erythroid and colony forming units-granulocyte macrophage of the treatment group were significantly higher than those in the control group(P0.01),but there was no significant difference in the colony forming units-fibroblast count between the two groups(P0.05).Conclusions:MSCs derived from human umbilical cord can play some poles on hemopoietic function of the bone marrow of AA mice.
Objective:To investigate the effects of umbilical cord mesenchymal stem cells(MSCs)on hemopoietic function of bone marrow in mice with aplastic anemia(AA).Methods:Umbilical cord MSCs were primarily cultured with adherent method.All mice were divided into the model group,control group and MSCs infusion group.The aplastic anemia model mice established by immune-mediated means were infused with MSCs through caudalis vena.The peripheral hemogram and bone marrow morphology were observed at 3,7,14,21 and 28 days.The level of hematopoiesis negative regulatory factor(IFN-)was detected by ELISA.The colony-forming unit counts of bone marrow hemopoietic progenitor cells were measured.Results:The spindle-shaped MSCs extended from the tissue edge at 8-10 days after primary adherent culture of umbilical cord,and those were parallelly or whirlpool-shape growth with the cultivation.The increasing peripheral hemogram and decreasing IFN- levels of AA mice treated with MSCs were significant(P0.01).The colony forming units-erythroid and colony forming units-granulocyte macrophage of the treatment group were significantly higher than those in the control group(P0.01),but there was no significant difference in the colony forming units-fibroblast count between the two groups(P0.05).Conclusions:MSCs derived from human umbilical cord can play some poles on hemopoietic function of the bone marrow of AA mice.
2013, 37(6): 652-654.
Abstract:
Objective:To investigate the effect of tea polyphenols(TP)on glyphosate induced mouse sertoli cell(SC)injury.Methods:The mouse sertoli cell was cultured and identified by oil red O.The cells were divided randomly into control group,glyphosate model group(the SC was treated with glyphosate at 90 g/ml)and TP pretreatment group(the SC was pretreated with 40,80,160 g/ml TP for 12 hours,then treated with glyphosate for 24 hours respectively).The cell viability was measured by metylthiazdyltetrazolium method,lactate dehydrogenase(LDH)activity was determined by colorimetric method,and the cell apoptosis was observed by TUNEL method.Results:In contrast to control group,the cell survival rate was decreased,LDH activity and cell apoptosis ratio were increased in glyphosate model group(P0.01).In TP pretreatment at 40 g/ml and 80g/ml groups,the cell survival rate was significantly improved,LDH release and cell apoptosis ratio were decreased(P0.01).In TP pretreatment at 160 g/ml group,there were no significant changes than that in glyphosate model group(P0.05).Conclusions:The appropriate concentrations of TP at 40 g/ml and 80 g/ml can protect sertoli cell against glyphosate induced injury.
Objective:To investigate the effect of tea polyphenols(TP)on glyphosate induced mouse sertoli cell(SC)injury.Methods:The mouse sertoli cell was cultured and identified by oil red O.The cells were divided randomly into control group,glyphosate model group(the SC was treated with glyphosate at 90 g/ml)and TP pretreatment group(the SC was pretreated with 40,80,160 g/ml TP for 12 hours,then treated with glyphosate for 24 hours respectively).The cell viability was measured by metylthiazdyltetrazolium method,lactate dehydrogenase(LDH)activity was determined by colorimetric method,and the cell apoptosis was observed by TUNEL method.Results:In contrast to control group,the cell survival rate was decreased,LDH activity and cell apoptosis ratio were increased in glyphosate model group(P0.01).In TP pretreatment at 40 g/ml and 80g/ml groups,the cell survival rate was significantly improved,LDH release and cell apoptosis ratio were decreased(P0.01).In TP pretreatment at 160 g/ml group,there were no significant changes than that in glyphosate model group(P0.05).Conclusions:The appropriate concentrations of TP at 40 g/ml and 80 g/ml can protect sertoli cell against glyphosate induced injury.
2013, 37(6): 655-657.
Abstract:
Objective:To explore the expression of breast cancer resistance protein(BCrP)and its significance in immortalized cell lines of tongue carcinoma with lymphatic metastasis.Methods:The immortalized cell line(Tca8113-Ml)was harvested by isolating and culturing from lymph node of lymphatic metastasis nude mice.The lymphatic metastasis model were established by injecting Tca8113-Ml into the nail pad of nude mice,and the control group were injected with stroke physiological saline solution.Lymph nodes of two groups were harvested after 4 weeks.The expression of BCrP in lymph node was detected by immunohistochemical stain.Results:The positive expression of BCrP in the lymph node of model group was observed by immunohistochemical staining,the positive rates of which was 27% to 35%.There was no expression of BCrP in the control group.Conclusions:Cancer stem cells areseed cellsof the metastasis and recurrence of cancer.The expression of stem cell marker BCrP in metastasizing lymph node of tongue carcinoma can provide the theory basis for further invesigating the cancer metastasis and drug resistance.
Objective:To explore the expression of breast cancer resistance protein(BCrP)and its significance in immortalized cell lines of tongue carcinoma with lymphatic metastasis.Methods:The immortalized cell line(Tca8113-Ml)was harvested by isolating and culturing from lymph node of lymphatic metastasis nude mice.The lymphatic metastasis model were established by injecting Tca8113-Ml into the nail pad of nude mice,and the control group were injected with stroke physiological saline solution.Lymph nodes of two groups were harvested after 4 weeks.The expression of BCrP in lymph node was detected by immunohistochemical stain.Results:The positive expression of BCrP in the lymph node of model group was observed by immunohistochemical staining,the positive rates of which was 27% to 35%.There was no expression of BCrP in the control group.Conclusions:Cancer stem cells areseed cellsof the metastasis and recurrence of cancer.The expression of stem cell marker BCrP in metastasizing lymph node of tongue carcinoma can provide the theory basis for further invesigating the cancer metastasis and drug resistance.
2013, 37(6): 658-660.
Abstract:
Objective:To observe in vivo effect of mixture penqiangyan on rabbit uterine smooth muscle movement andin vivoeffect of mixture penqiangyan on rat uterine smooth muscle movement.Methods:rabbits were grouped into control group,mixture penqiangyan groups(8.1,16.3 and 32.5 g/kg)and mixture fuyankangfu group(4.5g/kg)with duodenal administering.rats were grouped into control group,mixture penqiangyan groups(11.4,22.8 and 45.5g/kg)and mixture fuyankangfu group(7.0 g/kg)with oral administering twice a day for three days.Three days later,mixture penqiangyan containing serum was isolated from the rats and rat uterine isolated were incubated with mixture penqiangyan containing serumin vivo.Results:Sixteen point three g/kg and 32.5 g/kg of mixture penqiangyan significantly excite amplitude of rabbit uterine contractionin vivo(P0.05),and effect of 16.3 g/kg of mixture penqiangyan is better than that of 8.1g/kg.Further study showed that 91.0 g/kg of mixture penqiangyan containing serum significantly excite amplitude and activity of rat uterine contraction in vitro(P0.05-P0.01),while 45.5 g/kg of mixture penqiangyan containing serum only increased the amplitude of rat uterine contraction(P0.05).Conclusions:Mixture penqiangyan may improve symptoms of chronic pelvic inflammatory disease by exciting uterine smooth muscle.
Objective:To observe in vivo effect of mixture penqiangyan on rabbit uterine smooth muscle movement andin vivoeffect of mixture penqiangyan on rat uterine smooth muscle movement.Methods:rabbits were grouped into control group,mixture penqiangyan groups(8.1,16.3 and 32.5 g/kg)and mixture fuyankangfu group(4.5g/kg)with duodenal administering.rats were grouped into control group,mixture penqiangyan groups(11.4,22.8 and 45.5g/kg)and mixture fuyankangfu group(7.0 g/kg)with oral administering twice a day for three days.Three days later,mixture penqiangyan containing serum was isolated from the rats and rat uterine isolated were incubated with mixture penqiangyan containing serumin vivo.Results:Sixteen point three g/kg and 32.5 g/kg of mixture penqiangyan significantly excite amplitude of rabbit uterine contractionin vivo(P0.05),and effect of 16.3 g/kg of mixture penqiangyan is better than that of 8.1g/kg.Further study showed that 91.0 g/kg of mixture penqiangyan containing serum significantly excite amplitude and activity of rat uterine contraction in vitro(P0.05-P0.01),while 45.5 g/kg of mixture penqiangyan containing serum only increased the amplitude of rat uterine contraction(P0.05).Conclusions:Mixture penqiangyan may improve symptoms of chronic pelvic inflammatory disease by exciting uterine smooth muscle.
2013, 37(6): 661-662,665.
Abstract:
Objective:To investigate the variation of the immune functions in ulcerative colitis(UC)rats,including the immune globulin,complement and red blood cell(rBC).Methods:Forty-eight rats were randomly divided into control group model group 1,model group 2 and model group 3,with 12 rats in each.The model groups were given 0.5 ml 30% alcohol enema containing 20 mgtrinitrobenzene-sulfonic acid(TNBS)to construct UC rat models and the control group was given 0.5 ml physiological saline enema.The changes of the immune globulin complement and rBC immune function were detected.Results:The content of IgA and rBC circulating immune complexes in the blood serum of the model group increased predominantly at the first,third and seventh week as compared with those of the normal group(P0.01);the content of IgG in the model group increased predominantly at the first and third week as compared with that in the normal group(P0.01).The expressions of IgA,rBC circulating immune complexes and IgG were the highest in the model group at the first week,which began to decrease at the third one,and the expression at the seventh and the first week was statistically different(P0.01).IgM had no significant deviation between the model groups and the control group(P0.05),but C3 and red blood cell surface C3b receptor in the model group were lower than those in the control group at the first,third and seventh week(P0.01).The content of C4 in the model group was lower at the first and the third week than that in the control group(P0.01).Conclusions:The changes of immune globulin,complement and rBC immune function reveal that abnormal increasing of immunoresponse may lead to deposition of circulating immune complexes in the intestinal mucosa of the UC rats and activate local allergy.
Objective:To investigate the variation of the immune functions in ulcerative colitis(UC)rats,including the immune globulin,complement and red blood cell(rBC).Methods:Forty-eight rats were randomly divided into control group model group 1,model group 2 and model group 3,with 12 rats in each.The model groups were given 0.5 ml 30% alcohol enema containing 20 mgtrinitrobenzene-sulfonic acid(TNBS)to construct UC rat models and the control group was given 0.5 ml physiological saline enema.The changes of the immune globulin complement and rBC immune function were detected.Results:The content of IgA and rBC circulating immune complexes in the blood serum of the model group increased predominantly at the first,third and seventh week as compared with those of the normal group(P0.01);the content of IgG in the model group increased predominantly at the first and third week as compared with that in the normal group(P0.01).The expressions of IgA,rBC circulating immune complexes and IgG were the highest in the model group at the first week,which began to decrease at the third one,and the expression at the seventh and the first week was statistically different(P0.01).IgM had no significant deviation between the model groups and the control group(P0.05),but C3 and red blood cell surface C3b receptor in the model group were lower than those in the control group at the first,third and seventh week(P0.01).The content of C4 in the model group was lower at the first and the third week than that in the control group(P0.01).Conclusions:The changes of immune globulin,complement and rBC immune function reveal that abnormal increasing of immunoresponse may lead to deposition of circulating immune complexes in the intestinal mucosa of the UC rats and activate local allergy.
2013, 37(6): 663-665.
Abstract:
Objective:To evaluate the efficiency and safety of moxifloxacin on acute exacerbation of chronic obstructive pulmonary disease(AECOPD)in elderly patients.Methods:Seventy-eight elderly patients with AECOPD were randomly divided into observation group and control group.In control group,38 patients were treated with levofloxacin at 0.4g,intravenous infusion,one time per day;The 40 patients in observation group were treated with moxifloxacin at 0.4g intravenous infusion,one time per day;the treatment lasted 7-14 days in the two group,the total effective rate、bacterial eradication rate and drug reaction were compared between the two groups.Results:In moxifloxacin group,the effective rate was 87.5%,in levofloxacin group,the effective rate was 73.7%,there had statisticaldifferences(P0.05).The bacterial eradication rate and adverse drug reaction rate were 90.5% and 7.5% in moxifloxacin group vs 58.8% and 10.5% in levofloxacin group,which had no statistical differences(P0.05).Conclusions:Moxifloxacin is an effective and safe drug in treatment of AECOPD in elderly patients.
Objective:To evaluate the efficiency and safety of moxifloxacin on acute exacerbation of chronic obstructive pulmonary disease(AECOPD)in elderly patients.Methods:Seventy-eight elderly patients with AECOPD were randomly divided into observation group and control group.In control group,38 patients were treated with levofloxacin at 0.4g,intravenous infusion,one time per day;The 40 patients in observation group were treated with moxifloxacin at 0.4g intravenous infusion,one time per day;the treatment lasted 7-14 days in the two group,the total effective rate、bacterial eradication rate and drug reaction were compared between the two groups.Results:In moxifloxacin group,the effective rate was 87.5%,in levofloxacin group,the effective rate was 73.7%,there had statisticaldifferences(P0.05).The bacterial eradication rate and adverse drug reaction rate were 90.5% and 7.5% in moxifloxacin group vs 58.8% and 10.5% in levofloxacin group,which had no statistical differences(P0.05).Conclusions:Moxifloxacin is an effective and safe drug in treatment of AECOPD in elderly patients.
2013, 37(6): 666-668.
Abstract:
Objective:To evaluate the clinical value of retroperitoneal laparoscopy ureterolithotomy in treating upper ureteric calculus.Methods:The data of retroperitoneal laparoscopy ureterolithotomy in the treatment of 19 cases with upper ureteral calculus were retrospectively analysed.Results:All operations were successfully performed and the average operative time and blood loss were range from 120 to 180 minutes and range from 100 to 200 ml,respectively.During the follow-up of 1 to 36 months,no ureteric stricture was observed.Conclusions:retroperitoneal laparoscopy ureterolithotomy is a safe,reliable and minimally invasive way to treat upper ureteric calculus.
Objective:To evaluate the clinical value of retroperitoneal laparoscopy ureterolithotomy in treating upper ureteric calculus.Methods:The data of retroperitoneal laparoscopy ureterolithotomy in the treatment of 19 cases with upper ureteral calculus were retrospectively analysed.Results:All operations were successfully performed and the average operative time and blood loss were range from 120 to 180 minutes and range from 100 to 200 ml,respectively.During the follow-up of 1 to 36 months,no ureteric stricture was observed.Conclusions:retroperitoneal laparoscopy ureterolithotomy is a safe,reliable and minimally invasive way to treat upper ureteric calculus.
2013, 37(6): 668-670.
Abstract:
Objective:To investigate the curative effects of retroperitoneoscopy renal pedicle lymphatic dissection in the treatment of chyluria.Methods:The clinical data of 52 patients with chyluria treated with retroperitoneoscopy renal pedicle lymphatic dissection were retrospectively analysed.Results:Because of intraoperative vascular injury,2 cases were treated with open surgery.The remaining case's operations were successful.Their operation time and intraoperative blood loss were 95 to 150 minutes and 50 to 300 ml,respectively.There were gross hematuria in 12 patients after operation,which disappeared after treatment,no serious complication was found.The postoperative hospitalization time of all cases were 6 to 12 days,their urine was clear after discharge.The following-up time of 48 cases were from 6 months to 3 years.Four cases recurred during 6 months after operation,they were diagnosed as contralateral chyluria by cystoscopy,and cured after surgery.The remaining following-up patients didn' t recurred and renal atrophy or nephroptosis.Conclusions:retroperitoneoscopy renal pedicle lymphatic dissection in the treatment of chyluria is effective and minimal invasion.Improving surgical method can shorten the operation time and improve surgical safety.
Objective:To investigate the curative effects of retroperitoneoscopy renal pedicle lymphatic dissection in the treatment of chyluria.Methods:The clinical data of 52 patients with chyluria treated with retroperitoneoscopy renal pedicle lymphatic dissection were retrospectively analysed.Results:Because of intraoperative vascular injury,2 cases were treated with open surgery.The remaining case's operations were successful.Their operation time and intraoperative blood loss were 95 to 150 minutes and 50 to 300 ml,respectively.There were gross hematuria in 12 patients after operation,which disappeared after treatment,no serious complication was found.The postoperative hospitalization time of all cases were 6 to 12 days,their urine was clear after discharge.The following-up time of 48 cases were from 6 months to 3 years.Four cases recurred during 6 months after operation,they were diagnosed as contralateral chyluria by cystoscopy,and cured after surgery.The remaining following-up patients didn' t recurred and renal atrophy or nephroptosis.Conclusions:retroperitoneoscopy renal pedicle lymphatic dissection in the treatment of chyluria is effective and minimal invasion.Improving surgical method can shorten the operation time and improve surgical safety.
2013, 37(6): 671-673,676.
Abstract:
Objective:To evaluate the clinical effects of gastrointestinal motility drug combined with microecology on preventing feeding intolerance in premature infants.Methods:Four hundred and forty-eight premature infants were divided into group A(the conventional group,104 cases),group B(the microecological group,173 cases)and group C(the microecological and gastrointestinal motility group,171 cases).Group A were treated with routine nursing and therapy.Based on group A,group B were treated with microecological agent(duplex live bacteria powder of clostridium butyricum and infant bifidobacterium)for 0.5 g twice a day by oral or stomach tube within 6 hours after birth.Based on group B,Mosapride(gastrointestinal motility drug)was given to group C for 0.2 mg/kg three times a day.The incidence of feeding intolerance,decreasing degree of body weight,time of returning to birth weight,reaching full gastrointestinal feeding and hospital stay,and weight growth speed in three groups were investigatedResults:Compared with group A,the incidence of feeding intolerance,duration of feeding intolerance,body weight decreasing extent,time of reaching the birth body weight,full enteral feeding and hospital stay were less in group B and C,their weight growth speed were greater(P0.05 to 0.01).There was no statistically significant difference in the incidence of feeding intolerance between group C and B(P0.05).The time of duration of feeding intolerance returning to birth weight and full gastrointestinal feeding and hospital stay in group C were shorter than those in group B(P0.05 to 0.01).The body weight decreasing extent and weight growth speed in group C were lower and more than those in group B,respectively(P0.01).No adverse reactions were observed in the application process of gastrointestinal motility drug and microecology.Conclusions:Gastrointestinal motility drug combined with microecology can effectively prevent the feeding intolerance of premature infants,the effect is better than singly microecological agent,and can promote the early growth of premature infants and shorten the time of reaching full enteral feeding.
Objective:To evaluate the clinical effects of gastrointestinal motility drug combined with microecology on preventing feeding intolerance in premature infants.Methods:Four hundred and forty-eight premature infants were divided into group A(the conventional group,104 cases),group B(the microecological group,173 cases)and group C(the microecological and gastrointestinal motility group,171 cases).Group A were treated with routine nursing and therapy.Based on group A,group B were treated with microecological agent(duplex live bacteria powder of clostridium butyricum and infant bifidobacterium)for 0.5 g twice a day by oral or stomach tube within 6 hours after birth.Based on group B,Mosapride(gastrointestinal motility drug)was given to group C for 0.2 mg/kg three times a day.The incidence of feeding intolerance,decreasing degree of body weight,time of returning to birth weight,reaching full gastrointestinal feeding and hospital stay,and weight growth speed in three groups were investigatedResults:Compared with group A,the incidence of feeding intolerance,duration of feeding intolerance,body weight decreasing extent,time of reaching the birth body weight,full enteral feeding and hospital stay were less in group B and C,their weight growth speed were greater(P0.05 to 0.01).There was no statistically significant difference in the incidence of feeding intolerance between group C and B(P0.05).The time of duration of feeding intolerance returning to birth weight and full gastrointestinal feeding and hospital stay in group C were shorter than those in group B(P0.05 to 0.01).The body weight decreasing extent and weight growth speed in group C were lower and more than those in group B,respectively(P0.01).No adverse reactions were observed in the application process of gastrointestinal motility drug and microecology.Conclusions:Gastrointestinal motility drug combined with microecology can effectively prevent the feeding intolerance of premature infants,the effect is better than singly microecological agent,and can promote the early growth of premature infants and shorten the time of reaching full enteral feeding.
2013, 37(6): 674-676.
Abstract:
Objective:To investigate the value of serum retinol binding protein(srBP)and urinary N-acctyl-glucosaminidase(NAG)in the diagnosis of early-stage nephropathy in type 2 diabetes melitus.Methods:According to the ratio of microalbuminuria(mALB)to urine creatinine(UCr),120 patients with type 2 diabetes were divided into the control group(60 cases,mALB/UCr less than 30 mg/g)and observation group(60 cases,mALB/UCr from 30 to 299 mg/g).The levels of srBP and urinary NAG of all patients were detected.Results:The levels of srBP and urinary NAG and their detection positive rates in observation group were significantly higher than those in control group(P0.01),but the difference of the detection positive rate between the two indicators in observation group had no statistical significance.The levels of srBP and urinary NAG were positively correlated to the levels of urinary mALB in observation group(P0.01).Conclusions:The levels of srBP and urinary NAG can be a sensitive index for identifying the early renal damage of type 2 diabetes,which has important value in the diagnosis of early-stage nephropathy in type 2 diabetes melitus.
Objective:To investigate the value of serum retinol binding protein(srBP)and urinary N-acctyl-glucosaminidase(NAG)in the diagnosis of early-stage nephropathy in type 2 diabetes melitus.Methods:According to the ratio of microalbuminuria(mALB)to urine creatinine(UCr),120 patients with type 2 diabetes were divided into the control group(60 cases,mALB/UCr less than 30 mg/g)and observation group(60 cases,mALB/UCr from 30 to 299 mg/g).The levels of srBP and urinary NAG of all patients were detected.Results:The levels of srBP and urinary NAG and their detection positive rates in observation group were significantly higher than those in control group(P0.01),but the difference of the detection positive rate between the two indicators in observation group had no statistical significance.The levels of srBP and urinary NAG were positively correlated to the levels of urinary mALB in observation group(P0.01).Conclusions:The levels of srBP and urinary NAG can be a sensitive index for identifying the early renal damage of type 2 diabetes,which has important value in the diagnosis of early-stage nephropathy in type 2 diabetes melitus.
2013, 37(6): 677-679,683.
Abstract:
Objective:To investigate the association of urotensin Ⅱ(UⅡ)gene S89N polymorphism with type 2 diabetes mellitus(T2DM)and obesity.Methods:One hundred and five patients with T2DM(53 obesity,52 normal)and 114 non-diabetic subjects(55 obesity,59 normal)in Bengbu,Anhui province were randomly selected in the study.The polymerase chain reaction restriction fragment length polymorphism and the gel electrophoretic mobility shift assay were used to examine the S89N polymorphism.Results:The frequency of the Ser/Ser genotype was 64.9% in non-diabetic subjects and 52.4% in T2DM patients.The Asn/Asn frequencies were 5.3% and 16.2% in the two groups respectively.The difference was significant(P0.05).The genotype S89N of UⅡwas related with T2DM(P0.01),but not with obesity(P0.05).The difference of metabolic indexes of FPG,PBG,FINS INS2h,HOMA-Ir,ISI and TG among three genotypes of UⅡ in T2DM group was significant(P0.05-P0.01).The difference of metabolic indexes of CP and CP2h between Asn/Asn and the other two genotypes of UⅡ in T2DM group was significant(P0.01).Conclusions:The UⅡ gene S89N polymorphism is significantly associated with T2DM,but not with obesity among the Han population in Bengbu city.
Objective:To investigate the association of urotensin Ⅱ(UⅡ)gene S89N polymorphism with type 2 diabetes mellitus(T2DM)and obesity.Methods:One hundred and five patients with T2DM(53 obesity,52 normal)and 114 non-diabetic subjects(55 obesity,59 normal)in Bengbu,Anhui province were randomly selected in the study.The polymerase chain reaction restriction fragment length polymorphism and the gel electrophoretic mobility shift assay were used to examine the S89N polymorphism.Results:The frequency of the Ser/Ser genotype was 64.9% in non-diabetic subjects and 52.4% in T2DM patients.The Asn/Asn frequencies were 5.3% and 16.2% in the two groups respectively.The difference was significant(P0.05).The genotype S89N of UⅡwas related with T2DM(P0.01),but not with obesity(P0.05).The difference of metabolic indexes of FPG,PBG,FINS INS2h,HOMA-Ir,ISI and TG among three genotypes of UⅡ in T2DM group was significant(P0.05-P0.01).The difference of metabolic indexes of CP and CP2h between Asn/Asn and the other two genotypes of UⅡ in T2DM group was significant(P0.01).Conclusions:The UⅡ gene S89N polymorphism is significantly associated with T2DM,but not with obesity among the Han population in Bengbu city.
2013, 37(6): 680-683.
Abstract:
Objective:To investigate the correlation between serum procalcitonin(PCT)level in children with or without bacterial infection and clinical severity of disease.Methods:One hundred and seventy-nine children were divided into bacterial infection group(97 cases),virus infection group(59 cases)and uninfection group(23 cases).The levels of PCT in three groups were compared.Fifty-two cases in bacterial infection group who accorded wih the diagnostic criteria of systemic inflammatory response syndrome were assessed with pediatric critical illness score(PCIS).The correlation between PCT level and severity of clinical infection was observed.Results:The level of PCT in bacterial infection group was significantly higher than those in virus infection and uninfection group(P0.01).As the critical value for 0.5 ng/ml,the sensitivity and specificity of PCT in the diagnosis of bacterial infection were 80.41% and 82.93%,respectively.As the critical value for 2.0 ng/ml,the sensitivity and specificity of PCT in the diagnosis of bacterial infection were 30.93% and 98.78%,respectively.PCT level increased with PCIS scores decreasing.PCIS score was negatively correlated with PCT level,and positively correlated with the clinical severity of the disease(P0.01).Conclusions:PCT can be used to diagnose the early bacterial and non-bacterial infection,guide the rational use of antibiotics,warn whole body serious infection,guide clinical treatment and determine the prognosis of the disease.
Objective:To investigate the correlation between serum procalcitonin(PCT)level in children with or without bacterial infection and clinical severity of disease.Methods:One hundred and seventy-nine children were divided into bacterial infection group(97 cases),virus infection group(59 cases)and uninfection group(23 cases).The levels of PCT in three groups were compared.Fifty-two cases in bacterial infection group who accorded wih the diagnostic criteria of systemic inflammatory response syndrome were assessed with pediatric critical illness score(PCIS).The correlation between PCT level and severity of clinical infection was observed.Results:The level of PCT in bacterial infection group was significantly higher than those in virus infection and uninfection group(P0.01).As the critical value for 0.5 ng/ml,the sensitivity and specificity of PCT in the diagnosis of bacterial infection were 80.41% and 82.93%,respectively.As the critical value for 2.0 ng/ml,the sensitivity and specificity of PCT in the diagnosis of bacterial infection were 30.93% and 98.78%,respectively.PCT level increased with PCIS scores decreasing.PCIS score was negatively correlated with PCT level,and positively correlated with the clinical severity of the disease(P0.01).Conclusions:PCT can be used to diagnose the early bacterial and non-bacterial infection,guide the rational use of antibiotics,warn whole body serious infection,guide clinical treatment and determine the prognosis of the disease.
2013, 37(6): 684-687.
Abstract:
Objective:To investigate the effects of preemptive analgesia of parecoxib sodium on cellular immune function in perioperative period of patients with esophageal carcinoma.Methods:Fifty patients scheduled for esophageal carcinoma surgery were randomly divided into P and C group.Group P and C were injected with 5 ml of parecoxib sodium(40 mg)and normal saline at 30 minutes before anesthesia induction respectively.Two groups were treated with patient controlled epidural analgesia after operation.The levels of T-lymphocyte subsets(CD4+and CD8+)and T helper lymphocytes differetiation were measured by flow cytometry before anesthesia induction,at 2 hours after the start of operation and 24 and 48 hours after operation.Results:The differences of the levels of CD4+＆ CD8+,Th1 ＆ Th2 and ratio of Th1 and Th2 in two groups were not statistical significance before anesthesia(P0.05).Compared with before anesthesia,the levels of CD4+and CD4+/CD8+in two groups decreased at 2 hours after the start of operation and 24 and 48 hours after operation(P0.01).Compared with group C,the levels of CD4+at 24 and 48 hours after operation and CD4+/CD8+at 2 hours after the start of operation and 24 and 48 hours after operation in group P were higher(P0.05 to P0.01).Compared with before anesthesia,the levels of Th1 and ratio of Th1 and Th2 in two groups decreased at 24 and 48 hours after operation(P0.05 to P0.01),while the Th2 level significantly increased(P0.05 and P0.01).The ratio of Th1 and Th2 in group P was significantly higher than that in group C at 24 and 48 hours after operation(P0.05 and P0.01).Conclusions:Preemptive analgesia of parecoxib sodium may alleviate immunosuppression in tumor patients by the way of lowering the decreasing index of CD4+T lymphocyte,inhibiting the tendency of Th1/Th2 balance and activating Th2 immune response.
Objective:To investigate the effects of preemptive analgesia of parecoxib sodium on cellular immune function in perioperative period of patients with esophageal carcinoma.Methods:Fifty patients scheduled for esophageal carcinoma surgery were randomly divided into P and C group.Group P and C were injected with 5 ml of parecoxib sodium(40 mg)and normal saline at 30 minutes before anesthesia induction respectively.Two groups were treated with patient controlled epidural analgesia after operation.The levels of T-lymphocyte subsets(CD4+and CD8+)and T helper lymphocytes differetiation were measured by flow cytometry before anesthesia induction,at 2 hours after the start of operation and 24 and 48 hours after operation.Results:The differences of the levels of CD4+＆ CD8+,Th1 ＆ Th2 and ratio of Th1 and Th2 in two groups were not statistical significance before anesthesia(P0.05).Compared with before anesthesia,the levels of CD4+and CD4+/CD8+in two groups decreased at 2 hours after the start of operation and 24 and 48 hours after operation(P0.01).Compared with group C,the levels of CD4+at 24 and 48 hours after operation and CD4+/CD8+at 2 hours after the start of operation and 24 and 48 hours after operation in group P were higher(P0.05 to P0.01).Compared with before anesthesia,the levels of Th1 and ratio of Th1 and Th2 in two groups decreased at 24 and 48 hours after operation(P0.05 to P0.01),while the Th2 level significantly increased(P0.05 and P0.01).The ratio of Th1 and Th2 in group P was significantly higher than that in group C at 24 and 48 hours after operation(P0.05 and P0.01).Conclusions:Preemptive analgesia of parecoxib sodium may alleviate immunosuppression in tumor patients by the way of lowering the decreasing index of CD4+T lymphocyte,inhibiting the tendency of Th1/Th2 balance and activating Th2 immune response.
2013, 37(6): 688-690.
Abstract:
Objective:To observe the clinical efficacy of lactulose combined with live combined Bacillus subtilis and Enterococcus faecium granules with multivitamines(Mamiai)in neonatal jaundice therapy and explore its therapeutic mechanism.Methods:One hundred and twenty cases of neonatal jaundice were randomly divided into three groups,blue-ray therapy was used in control group;in group A,Mamiai was added on the basis of the control group,and in group B,lactulose combined with Mamiai was added on the basis of the control group.Results:Compared with group A,the effective rate in group B was improved(P0.05).Compared with control group,neonatal blood total bilirubin content was significantly lower in group A and group B after 6 days treatment(P0.01).Compared with group A,neonatal blood total bilirubin content in group B was lower(P0.05).Conclusions:Lactulose combined with Mamiai reduces serum bilirubin level through two different mechanisms,the efficacy for treatment of neonatal jaundice was reliable,safe,and worthy of clinical use.
Objective:To observe the clinical efficacy of lactulose combined with live combined Bacillus subtilis and Enterococcus faecium granules with multivitamines(Mamiai)in neonatal jaundice therapy and explore its therapeutic mechanism.Methods:One hundred and twenty cases of neonatal jaundice were randomly divided into three groups,blue-ray therapy was used in control group;in group A,Mamiai was added on the basis of the control group,and in group B,lactulose combined with Mamiai was added on the basis of the control group.Results:Compared with group A,the effective rate in group B was improved(P0.05).Compared with control group,neonatal blood total bilirubin content was significantly lower in group A and group B after 6 days treatment(P0.01).Compared with group A,neonatal blood total bilirubin content in group B was lower(P0.05).Conclusions:Lactulose combined with Mamiai reduces serum bilirubin level through two different mechanisms,the efficacy for treatment of neonatal jaundice was reliable,safe,and worthy of clinical use.
2013, 37(6): 690-691.
Abstract:
Objective:To observe the effects of exercise prescription on physical intervention.Methods:One hundred and fifty-three participants were selected from 4 elderly fitness teams of Shanghai Hongkou district.The targeted exercise prescription was formulated in detail according to their physical condition and exercise load.Body mass index,waistlines,hiplines,body fat percentage and the level of blood-lipid of all participants were compared in 6 months after intervention.Results:The differences of the serum total cholesterol and body mass index levels between before and after exercise were not statistical significance(P0.05).The differences of triacylglycerol,low density lipoprotein and body mass index between before and after exercise were statistical significance(P0.05-P0.01).Conclusions:Effective and scientific exercise can improve the related physiological and biochemical indexes of physique,and can reduce the incidence of chronic diseases.
Objective:To observe the effects of exercise prescription on physical intervention.Methods:One hundred and fifty-three participants were selected from 4 elderly fitness teams of Shanghai Hongkou district.The targeted exercise prescription was formulated in detail according to their physical condition and exercise load.Body mass index,waistlines,hiplines,body fat percentage and the level of blood-lipid of all participants were compared in 6 months after intervention.Results:The differences of the serum total cholesterol and body mass index levels between before and after exercise were not statistical significance(P0.05).The differences of triacylglycerol,low density lipoprotein and body mass index between before and after exercise were statistical significance(P0.05-P0.01).Conclusions:Effective and scientific exercise can improve the related physiological and biochemical indexes of physique,and can reduce the incidence of chronic diseases.
2013, 37(6): 692-695.
Abstract:
Objective:To evaluate the clinical effects and reliability of one-visit endodontic therapy for infected root canals.Methods:One hundred and forty-two teeth with dental pulp necrosis or chronic periapical periodontitis were investigated.Eighty teeth(one-visit group)were treated with one-visit canal obturation,and 62 teeth(two-visit group)were treated with canal obturation after two-visit therapy,the first therapy of which was sealing the root canals using calcium hydroxide.The pre-and post-operative pain in 1 week of two groups were observed,the 2 years cure rate after operation of two groups were evaluated,and the clinical effects of two groups were compared.Results:The differences of pre-and post-operative pain in 1 week of two groups were not statistical significance(P0.05).The 2 years cure rate after operation of one and two-visit group were 92.8% and 90.2%,respectively.The differences of curative effects in two groups were not statistically significant(P0.05).Conclusions:Compared with two-visit therapy,the postoperative pain and 2 years cure rate of one-visit endodontic therapy for infected root canals has no significant difference and its pain period is short,and which has a certain clinical feasibility.
Objective:To evaluate the clinical effects and reliability of one-visit endodontic therapy for infected root canals.Methods:One hundred and forty-two teeth with dental pulp necrosis or chronic periapical periodontitis were investigated.Eighty teeth(one-visit group)were treated with one-visit canal obturation,and 62 teeth(two-visit group)were treated with canal obturation after two-visit therapy,the first therapy of which was sealing the root canals using calcium hydroxide.The pre-and post-operative pain in 1 week of two groups were observed,the 2 years cure rate after operation of two groups were evaluated,and the clinical effects of two groups were compared.Results:The differences of pre-and post-operative pain in 1 week of two groups were not statistical significance(P0.05).The 2 years cure rate after operation of one and two-visit group were 92.8% and 90.2%,respectively.The differences of curative effects in two groups were not statistically significant(P0.05).Conclusions:Compared with two-visit therapy,the postoperative pain and 2 years cure rate of one-visit endodontic therapy for infected root canals has no significant difference and its pain period is short,and which has a certain clinical feasibility.
2013, 37(6): 695-697.
Abstract:
Objective:To explore the causes diagnosis,treatment and prevention of haemorrhage after pancreaticoduodenectomy(PD).Methods:The clinical data of 9 patients undergoing PD were retrospectively analyzed.The diagnosis of haemorrhage was made according to the definition and classification of postpancreatectomy haemorrhage(PPH)proposed by the international study group of the pancreatic surgery.Results:Early PPH occurred in 3 cases and delayed PPH in 6 cases;mild PPH was observed in 2 cases and severe PPH in 7 cases including 7 intraluminal PPH and 2 extraluminal PPH;5 patients received reoperation,1 patient emergency selective arterial embolism and 3 patients conservative therapy;2 patients died.Conclusions:Timely operation is essential for management of early PPH;delayed haemorrhage failed by conservative therapy should resort to emergency operation;meticulousoperative techniques,complete hemostasis and effective prevention of postoperative complications such as stomal leak could significantly reduce the occurrence of PPH.Selective arterial embolism might be employed for treatment of delayed hemorrhage.
Objective:To explore the causes diagnosis,treatment and prevention of haemorrhage after pancreaticoduodenectomy(PD).Methods:The clinical data of 9 patients undergoing PD were retrospectively analyzed.The diagnosis of haemorrhage was made according to the definition and classification of postpancreatectomy haemorrhage(PPH)proposed by the international study group of the pancreatic surgery.Results:Early PPH occurred in 3 cases and delayed PPH in 6 cases;mild PPH was observed in 2 cases and severe PPH in 7 cases including 7 intraluminal PPH and 2 extraluminal PPH;5 patients received reoperation,1 patient emergency selective arterial embolism and 3 patients conservative therapy;2 patients died.Conclusions:Timely operation is essential for management of early PPH;delayed haemorrhage failed by conservative therapy should resort to emergency operation;meticulousoperative techniques,complete hemostasis and effective prevention of postoperative complications such as stomal leak could significantly reduce the occurrence of PPH.Selective arterial embolism might be employed for treatment of delayed hemorrhage.
2013, 37(6): 698-699.
Abstract:
Objective:To summarize the experiences of early ultra low craniotomy decompression operation on severe craniocerebral injury.Methods:With the pterional approach for the foundation,expanding the operation range to prefrontal parietal and temporal cortices according to the injury lesions need,releasing skull base vacuum space,and preventing vicious cycle of intracranial pressure asearly as possible.Results:According to Glasgow healing standard evaluation the results showed good in 22 cases moderate disability in 9 cases,plant survival in 5 cases,14 cases were died,the mortality was 28%.Two of them were died of combined injury,3 patients were died of giving up treatment,3 patients were died of uncontrollable malignant intracranial hypertension,4 patients were died of pulmonary infection,1 patient was died of stress ulcer,1 patient was died of internal environment disturbance.Conclusions:Ultra low craniectomy decompression is an ideal method which can prevent vicious spiral of intracranial pressure early with sufficient and effective decompression.
Objective:To summarize the experiences of early ultra low craniotomy decompression operation on severe craniocerebral injury.Methods:With the pterional approach for the foundation,expanding the operation range to prefrontal parietal and temporal cortices according to the injury lesions need,releasing skull base vacuum space,and preventing vicious cycle of intracranial pressure asearly as possible.Results:According to Glasgow healing standard evaluation the results showed good in 22 cases moderate disability in 9 cases,plant survival in 5 cases,14 cases were died,the mortality was 28%.Two of them were died of combined injury,3 patients were died of giving up treatment,3 patients were died of uncontrollable malignant intracranial hypertension,4 patients were died of pulmonary infection,1 patient was died of stress ulcer,1 patient was died of internal environment disturbance.Conclusions:Ultra low craniectomy decompression is an ideal method which can prevent vicious spiral of intracranial pressure early with sufficient and effective decompression.
2013, 37(6): 700-702.
Abstract:
Objective:To observe the correlation between the plasma level of high sensitivity C creactive protein(Hs-C P),homocysteine(HCY)and pulse wave velocity(PWV)in patients with coronary heart disease(CHD).Methods:Fifty-seven CHD patients confirmed by coronary angioplasty were divided into single vessel-lesion group(14 cases) double-vessel-lesion group(26 cases)and triple-vessel-lesion group(17 cases),while 25 healthy adults were selected as control.The Hs-CrP,HCY,plasma glucose(FPG),triglyceride(TG) total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C)and PWV were detected in each group.Results:Compared with the control,the plasma level of FPG,TG,TC,LDL-C,Hs-CrP,HCY and PWV of the CAD group increased significantly(P0.01).The plasma level of Hs-CrP,HCY and PWV increased in accordance with the number of the coronary lesion(P0.05 to P0.01).The plasma level of HCY was positively correlated with that of Hs-CrP,HCY and PWV(P0.05).Conclusions:The plasma level of Hs-CrP,HCY and PWV are high in patients with CHD and closely correlated with the degree of the coronary lesion,which may be of use in predicting the severity of the CHD.
Objective:To observe the correlation between the plasma level of high sensitivity C creactive protein(Hs-C P),homocysteine(HCY)and pulse wave velocity(PWV)in patients with coronary heart disease(CHD).Methods:Fifty-seven CHD patients confirmed by coronary angioplasty were divided into single vessel-lesion group(14 cases) double-vessel-lesion group(26 cases)and triple-vessel-lesion group(17 cases),while 25 healthy adults were selected as control.The Hs-CrP,HCY,plasma glucose(FPG),triglyceride(TG) total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C)and PWV were detected in each group.Results:Compared with the control,the plasma level of FPG,TG,TC,LDL-C,Hs-CrP,HCY and PWV of the CAD group increased significantly(P0.01).The plasma level of Hs-CrP,HCY and PWV increased in accordance with the number of the coronary lesion(P0.05 to P0.01).The plasma level of HCY was positively correlated with that of Hs-CrP,HCY and PWV(P0.05).Conclusions:The plasma level of Hs-CrP,HCY and PWV are high in patients with CHD and closely correlated with the degree of the coronary lesion,which may be of use in predicting the severity of the CHD.
2013, 37(6): 702-704.
Abstract:
Objective:To explore the rate and indications of cesarean section in the past seven years.Methods:The clinical data of 5 685 puerpera of hospital childbirth were retrospectively analysed.The puerpera undergoned cesarean sectionwere 3 272 cases.Results:The rate of cesarean section increased with years,which was 44.94% in 2005 and 68.23% in 2011,and their difference was statistically significant(P0.01).Social factors,fetal distress,dystocia,pregnancy complications,scar uterus and breech presentation were the main indications of cesarean section,and the first factor of cesarean section was social factors.Conclusions:Strictly controlling indications,strengthening monitoring during pregnancy and labor observation,improving the understanding of medical staff and puerpera,actively deal with dystocia and reducing social factors are the key factors to reduce the rate of cesarean section.
Objective:To explore the rate and indications of cesarean section in the past seven years.Methods:The clinical data of 5 685 puerpera of hospital childbirth were retrospectively analysed.The puerpera undergoned cesarean sectionwere 3 272 cases.Results:The rate of cesarean section increased with years,which was 44.94% in 2005 and 68.23% in 2011,and their difference was statistically significant(P0.01).Social factors,fetal distress,dystocia,pregnancy complications,scar uterus and breech presentation were the main indications of cesarean section,and the first factor of cesarean section was social factors.Conclusions:Strictly controlling indications,strengthening monitoring during pregnancy and labor observation,improving the understanding of medical staff and puerpera,actively deal with dystocia and reducing social factors are the key factors to reduce the rate of cesarean section.
2013, 37(6): 705-706.
Abstract:
Objective:To observe the clinical effects of dexmedetomidine given by total intra venous anaesthesia igject(TIVAI)mode in preventing shivering during low epidural anesthesia.Methods:Sixty ASA Ⅰ or Ⅱ patients receiving low epidural anesthesia were randomly divided into observation group and control group by single blind method.In the observation group dexmedetomidine was given by TIVAI mode at a concentration of 0.8 g/kg within 15 minutes,and then dexmedetomidine was infused consistently at the rate of 0.2 gkg-1h-1until the end of operation;while in control group,0.9% sodium chloride injection was given by TIVAI mode instead.Results:The incidence and the extent of shivering in the control group was significantly higher than that in the observation group(P0.01);after the operation,more patients in the control group complained of discomfort during operation(P0.01).And 36.7% of them was not relieved until intervention was applied.Conclusions:Dexmedetomidine given by TIVAI mode can effectively prevent the incidence of shivering during low epidural anesthesia and make the patients feel peaceful and comfortable,which adapts to the current requirements of comfortable medical treatment.
Objective:To observe the clinical effects of dexmedetomidine given by total intra venous anaesthesia igject(TIVAI)mode in preventing shivering during low epidural anesthesia.Methods:Sixty ASA Ⅰ or Ⅱ patients receiving low epidural anesthesia were randomly divided into observation group and control group by single blind method.In the observation group dexmedetomidine was given by TIVAI mode at a concentration of 0.8 g/kg within 15 minutes,and then dexmedetomidine was infused consistently at the rate of 0.2 gkg-1h-1until the end of operation;while in control group,0.9% sodium chloride injection was given by TIVAI mode instead.Results:The incidence and the extent of shivering in the control group was significantly higher than that in the observation group(P0.01);after the operation,more patients in the control group complained of discomfort during operation(P0.01).And 36.7% of them was not relieved until intervention was applied.Conclusions:Dexmedetomidine given by TIVAI mode can effectively prevent the incidence of shivering during low epidural anesthesia and make the patients feel peaceful and comfortable,which adapts to the current requirements of comfortable medical treatment.
2013, 37(6): 707-709.
Abstract:
Objective:To evaluate the curative effects of laparoscopic assisted transvaginal hysterectomy(LAVH)combined with suspension surgery at the top of vagina in the treatment of postmenopausal uterine prolapse.Methods:Twenty-one patients with uterine prolapse treated with LAVH combined with suspension surgery at the top of vagina were set as the observation group,15 patients with uterine prolapse treated with transvaginal hysterectomy were set as the control group.The operation time amount of bleeding,complications and life improvement after following up for 24 months in two groups were compared.Results:The operation time,amount of bleeding and hospitalization and postoperative abdominal straining feeling,time of a full bladder and bulging vaginal fornix between two groups had statistical significance(P0.01).Conclusions:LAVH combined with suspension surgery at the top of vagina in the treatment of postmenopausal uterine prolapse is less trauma,good effects and qucik recovery.
Objective:To evaluate the curative effects of laparoscopic assisted transvaginal hysterectomy(LAVH)combined with suspension surgery at the top of vagina in the treatment of postmenopausal uterine prolapse.Methods:Twenty-one patients with uterine prolapse treated with LAVH combined with suspension surgery at the top of vagina were set as the observation group,15 patients with uterine prolapse treated with transvaginal hysterectomy were set as the control group.The operation time amount of bleeding,complications and life improvement after following up for 24 months in two groups were compared.Results:The operation time,amount of bleeding and hospitalization and postoperative abdominal straining feeling,time of a full bladder and bulging vaginal fornix between two groups had statistical significance(P0.01).Conclusions:LAVH combined with suspension surgery at the top of vagina in the treatment of postmenopausal uterine prolapse is less trauma,good effects and qucik recovery.
2013, 37(6): 710-713.
Abstract:
Objective:To observe the change of coagulation-related indicators levels(fibrinogen,fibrin degradationproductsand D-dimer)of different doses of recombinant tissue plasmiogen activator(rt-PA)in the treatment of acute cerebral infarction in middle cerebral artery zone.Methods:Eighty patients with acute cerebral infarction were treated with intravenous thrombolysis after 7 hours,and randomly divided into rt-PA 0.8 mg/kg and rt-PA 0.9 mg/kg group(40 cases each group).The levels of coagulation-related indicators(including fibrinogen,fibrin degradation products,D-dimer,clotting factor ⅩⅢ,2-antiplasmin plasminogen and platelet)in two groups were observed at 2 hours before thrombolysis and at 2 and 24 hours after thrombolysis.Results:The level differences of fibrinogen,fibrin degradation products,D-dimer,clotting factor Ⅲ,2-antiplasmin,plasminogen and platelet in two groups were not statistical significance(P0.05).Conclusions:There is no evident difference in the coagulation-related indicators between two groups,the 0.8 mg/kg can be selected to treat acute cerebral infarction.
Objective:To observe the change of coagulation-related indicators levels(fibrinogen,fibrin degradationproductsand D-dimer)of different doses of recombinant tissue plasmiogen activator(rt-PA)in the treatment of acute cerebral infarction in middle cerebral artery zone.Methods:Eighty patients with acute cerebral infarction were treated with intravenous thrombolysis after 7 hours,and randomly divided into rt-PA 0.8 mg/kg and rt-PA 0.9 mg/kg group(40 cases each group).The levels of coagulation-related indicators(including fibrinogen,fibrin degradation products,D-dimer,clotting factor ⅩⅢ,2-antiplasmin plasminogen and platelet)in two groups were observed at 2 hours before thrombolysis and at 2 and 24 hours after thrombolysis.Results:The level differences of fibrinogen,fibrin degradation products,D-dimer,clotting factor Ⅲ,2-antiplasmin,plasminogen and platelet in two groups were not statistical significance(P0.05).Conclusions:There is no evident difference in the coagulation-related indicators between two groups,the 0.8 mg/kg can be selected to treat acute cerebral infarction.
2013, 37(6): 713-715.
Abstract:
Objective:To observe the effect of macrolides in treatment of infant asthmatic respiratory diseases.Methods:One hundred and fifty infants with asthmatic diseases were randomly divided into two groups.The 80 cases in the treatment group were administered macrolides antibiotics and the 70 cases in control group received -lactam antibiotics.The curative effects were compared between the two groups.Results:The total effective rate was 86.25% in the treatment group and 70.00% in the control(P0.05).The symptom release time and length of hospital stay in the treatment group were less than those of the control(P0.05-P0.01).All the patients were followed up for one year.The recurrence rate was 40.00% in the treatment group and 58.57% in the control group,the difference was statistically significant(P0.05).Conclusions:Macrolides is effective on infant asthmatic respiratory diseases and the prognosis is satisfactory.It is worth widely popularizing.
Objective:To observe the effect of macrolides in treatment of infant asthmatic respiratory diseases.Methods:One hundred and fifty infants with asthmatic diseases were randomly divided into two groups.The 80 cases in the treatment group were administered macrolides antibiotics and the 70 cases in control group received -lactam antibiotics.The curative effects were compared between the two groups.Results:The total effective rate was 86.25% in the treatment group and 70.00% in the control(P0.05).The symptom release time and length of hospital stay in the treatment group were less than those of the control(P0.05-P0.01).All the patients were followed up for one year.The recurrence rate was 40.00% in the treatment group and 58.57% in the control group,the difference was statistically significant(P0.05).Conclusions:Macrolides is effective on infant asthmatic respiratory diseases and the prognosis is satisfactory.It is worth widely popularizing.
2013, 37(6): 716-717.
Abstract:
Objective:To investigate the clinical effects of Titanium cable combined with patella claw in the treatment of comminuted patellar fracture.Methods:The clinical data of twenty-three cases with comminuted patellar fracture treated with Titanium cable combined with patella claw internal fixation were retrospectively analyzed.Results:All cases were followed up for 1 to 2 years.All fractures healed,the function of knee joint was good,the patella claw was not fault and falling off,and the internal fixation was not loosening.Conclusions:Titanium cable combined with patella claw internal fixation for the treatment of patella fracture is easy to operate and reliable fixation,and the fracture healing is quick.Patients can early exercise,and the functional restoration of knee joint is good.
Objective:To investigate the clinical effects of Titanium cable combined with patella claw in the treatment of comminuted patellar fracture.Methods:The clinical data of twenty-three cases with comminuted patellar fracture treated with Titanium cable combined with patella claw internal fixation were retrospectively analyzed.Results:All cases were followed up for 1 to 2 years.All fractures healed,the function of knee joint was good,the patella claw was not fault and falling off,and the internal fixation was not loosening.Conclusions:Titanium cable combined with patella claw internal fixation for the treatment of patella fracture is easy to operate and reliable fixation,and the fracture healing is quick.Patients can early exercise,and the functional restoration of knee joint is good.
2013, 37(6): 718-720.
Abstract:
Objective:To observe the the effect of Toric intraocular lens implantation on treating aged-cataract.Methods:The monocular cataract of 64 senile patients with astigmatism between 0.75 and 2.5 diopter were extracted.The observation group(34 cases)and control group(30 cases)were implanted with Toric and common IOL respectively.The eyesight at 1 day before and after operation and 3 months after operation the best corrected visual acuity(BCVA)and postoperative residual refractive error of all patients were recorded.Results:The eyesight differences of two group at 1 day before and after operation were not statistically significant(P0.05).At 3 months after operation the eyesight differences of two group were statistically significant(P0.01)and their BCVA were not statistically significant(P0.05).The postoperative astigmatism of the observation group was significantly lower than that of the control group(P0.01).The 90% rotation angle of the observation group was less than 5 degree.Conclusions:The effect of Toric intraocular lens implantation on treating aged-cataract is better than traditional IOL,which is more suitable for cataract patients with moderate astigmatism.
Objective:To observe the the effect of Toric intraocular lens implantation on treating aged-cataract.Methods:The monocular cataract of 64 senile patients with astigmatism between 0.75 and 2.5 diopter were extracted.The observation group(34 cases)and control group(30 cases)were implanted with Toric and common IOL respectively.The eyesight at 1 day before and after operation and 3 months after operation the best corrected visual acuity(BCVA)and postoperative residual refractive error of all patients were recorded.Results:The eyesight differences of two group at 1 day before and after operation were not statistically significant(P0.05).At 3 months after operation the eyesight differences of two group were statistically significant(P0.01)and their BCVA were not statistically significant(P0.05).The postoperative astigmatism of the observation group was significantly lower than that of the control group(P0.01).The 90% rotation angle of the observation group was less than 5 degree.Conclusions:The effect of Toric intraocular lens implantation on treating aged-cataract is better than traditional IOL,which is more suitable for cataract patients with moderate astigmatism.
2013, 37(6): 721-723,726.
Abstract:
Objective:To investigate the effects the butorphanol on inhibiting the cardioascular response and dysphoria during the decannulation period of general anesthesia in patients with gynecologic laparoscopy.Methods:Ninety patients scheduled for gynecologic laparoscopy under general anesthesia were randomly divided into group B1,B2 and C.Group B1,B2 and C were treated with 1 mg butorphanol,Patient Controlled Analgesia pump(total volume 100 ml including 10 mg of butorphanol,0.3 mg of ramosetron Hydrochloride and normal saline(NS)for 2 ml per hour and 1 ml of NS by intravenous injection 30 minutes before the end of operation,respectively.Mean arterial pressure(MAP),heart rate(Hr)and saturation of peripheral oxygen(SpO2)of three groups were measured at 0 min(T1and T3)before and after surgery,extubation(T3),5 mins(T4)after extubation.The time of extubation and recovery,the incidence of dysphoria and adverse reactions were recorded.Results:MAP of group B1 was significantly lower than that of group B2 and C at T3(P0.01),there was no significant difference at T1(P0.05).Hr of group B1 was significantly lower than that of group B2 and C at T3(P0.01),and it was significantly higher than that at T2(P0.01).SpO2 of group B1 and C at T3were significantly lower than that at T1(P0.01).The differences of SpO2 and extubation time of three groups were no significant difference(P0.05).The incidence of no or mild dysphoria of group B1 was significantly higher than that in group B2 and C,but its incidence of moderate to severe dysphoria was significantly lower(P0.01).Conclusions:Bulorphanol can effectively inhibit the cardioascular response and reduce the incidence and degree of dysphoria during decannulation period of general anesthesia in patients with gynecologic laparoscopy.
Objective:To investigate the effects the butorphanol on inhibiting the cardioascular response and dysphoria during the decannulation period of general anesthesia in patients with gynecologic laparoscopy.Methods:Ninety patients scheduled for gynecologic laparoscopy under general anesthesia were randomly divided into group B1,B2 and C.Group B1,B2 and C were treated with 1 mg butorphanol,Patient Controlled Analgesia pump(total volume 100 ml including 10 mg of butorphanol,0.3 mg of ramosetron Hydrochloride and normal saline(NS)for 2 ml per hour and 1 ml of NS by intravenous injection 30 minutes before the end of operation,respectively.Mean arterial pressure(MAP),heart rate(Hr)and saturation of peripheral oxygen(SpO2)of three groups were measured at 0 min(T1and T3)before and after surgery,extubation(T3),5 mins(T4)after extubation.The time of extubation and recovery,the incidence of dysphoria and adverse reactions were recorded.Results:MAP of group B1 was significantly lower than that of group B2 and C at T3(P0.01),there was no significant difference at T1(P0.05).Hr of group B1 was significantly lower than that of group B2 and C at T3(P0.01),and it was significantly higher than that at T2(P0.01).SpO2 of group B1 and C at T3were significantly lower than that at T1(P0.01).The differences of SpO2 and extubation time of three groups were no significant difference(P0.05).The incidence of no or mild dysphoria of group B1 was significantly higher than that in group B2 and C,but its incidence of moderate to severe dysphoria was significantly lower(P0.01).Conclusions:Bulorphanol can effectively inhibit the cardioascular response and reduce the incidence and degree of dysphoria during decannulation period of general anesthesia in patients with gynecologic laparoscopy.
2013, 37(6): 724-726.
Abstract:
Objective:To evaluate the efficacy and safety of ultrasound guided percutaneous nephrolithotomy(PCNL)with the aid of pneumatic and ultrasonic power in treatment of renal calculi.Methods:One hundred and twenty-eight cases of renal calculi were treated by PCNL combined with pneumatic and ultrasonic lithotriptic system(EMS Ⅳ)under ultrasound guidance.Results:The 24F standard percutaneous renal access was successfully established in all the cases,and two accesses were established in 8 of them.The operative time ranged from 40 to 150 minutes.The stone-free rate was 82.0%(105/128)after the first operation,and no serious complications occurred.Stones were found in 23 cases and 15 of them underwent the PCNL for the second time;the stone-free rate was 86.7%(13/15)after the second operation.The total stone-free rate was 92.2%(118/128).Severe hemorrhage occurred in 8 cases after the operation;6 of them were cured by conservative therapy and the other 2 were performed nephrectomy.No septic shock or death occurred.Conclusions:Ultrasound guided percutaneous nephrolithotomy combined with pneumatic and ultrasonic lithotriptic system is safe and effective for treatment of the renal calculi.It is worth popularizing.
Objective:To evaluate the efficacy and safety of ultrasound guided percutaneous nephrolithotomy(PCNL)with the aid of pneumatic and ultrasonic power in treatment of renal calculi.Methods:One hundred and twenty-eight cases of renal calculi were treated by PCNL combined with pneumatic and ultrasonic lithotriptic system(EMS Ⅳ)under ultrasound guidance.Results:The 24F standard percutaneous renal access was successfully established in all the cases,and two accesses were established in 8 of them.The operative time ranged from 40 to 150 minutes.The stone-free rate was 82.0%(105/128)after the first operation,and no serious complications occurred.Stones were found in 23 cases and 15 of them underwent the PCNL for the second time;the stone-free rate was 86.7%(13/15)after the second operation.The total stone-free rate was 92.2%(118/128).Severe hemorrhage occurred in 8 cases after the operation;6 of them were cured by conservative therapy and the other 2 were performed nephrectomy.No septic shock or death occurred.Conclusions:Ultrasound guided percutaneous nephrolithotomy combined with pneumatic and ultrasonic lithotriptic system is safe and effective for treatment of the renal calculi.It is worth popularizing.
2013, 37(6): 727-728,731.
Abstract:
Objective:To explore the risk factors for rectovaginal fistula following total mesorectal excision in elderly female patients with rectal cancer,and to provide theoretical basis for the prevention.Methods:Thirty-six elderly female patients suffering from rectovaginal fistula after total mesorectal excision for rectal cancer were evaluated by single factor inspection and logistic regression analysis.Results:The factors related to the occurrence of rectovaginal fistula were as follows:the tumor was located in the rectum anterolateral(Or=2.413),the double stapling was used in the operation(Or=2.171)and the distance from tumor to anal was less than 10 cm(Or=1.833);the other factors involved were tumor stage(Or=1.547),preoperative radiotherapy(Or=1.233)and menopausal(Or=1.043).Conclusions:The tumor in the anterolateral position,use of the double stapling,distance of tumor to anal less than 10 cm,tumor stage,preoperative radiotherapy and meopausal are all the risk factors for rectovaginal fistula.Clinicians should take active measures to prevent the occurrence.
Objective:To explore the risk factors for rectovaginal fistula following total mesorectal excision in elderly female patients with rectal cancer,and to provide theoretical basis for the prevention.Methods:Thirty-six elderly female patients suffering from rectovaginal fistula after total mesorectal excision for rectal cancer were evaluated by single factor inspection and logistic regression analysis.Results:The factors related to the occurrence of rectovaginal fistula were as follows:the tumor was located in the rectum anterolateral(Or=2.413),the double stapling was used in the operation(Or=2.171)and the distance from tumor to anal was less than 10 cm(Or=1.833);the other factors involved were tumor stage(Or=1.547),preoperative radiotherapy(Or=1.233)and menopausal(Or=1.043).Conclusions:The tumor in the anterolateral position,use of the double stapling,distance of tumor to anal less than 10 cm,tumor stage,preoperative radiotherapy and meopausal are all the risk factors for rectovaginal fistula.Clinicians should take active measures to prevent the occurrence.
2013, 37(6): 729-731.
Abstract:
Objective:To evaluate the clinical effects of bladder instillation with mitomycin C(MMC)and pirarubicin on preventing the postoperative recurrence of superficial bladder cancer.Methods:A total of 126 patients with superficial bladder cancer were randomly divided into MMC and pirarubicin group.The MMC group were treated with 30 mg of MMC and 30 ml of 0.9% sodium chloride injection through bladder instillation for 2 hours.The pirarubicin group were treated with 30 mg of pirarubicin and 30 ml of 5% glucose injection through bladder instillation for 30 minutes.The safety and postoperative recurrence of superficial bladder cancer in two groups were observed.Results:All patients were followed up for 24 months.The recurrence rates of MMC and pirarubicin group were 14.3% and 13.7%,the difference of which had no statistical significance and the tumour progression of two groups had no statistical significance(P0.05).The incidence rates of chemical cystitis in MMC and pirarubicin group were 32.0% and 38.5%,the difference of which had no statistical significance(P0.05).The adverse drug reactions of two groups were not obvious.Conclusions:The clinical effects of bladder instillation with MMC and pirarubicin on preventing the postoperative recurrence of superficial bladder cancer are similar.These two drugs can be used as the preferred drug for clinical bladder instillation.
Objective:To evaluate the clinical effects of bladder instillation with mitomycin C(MMC)and pirarubicin on preventing the postoperative recurrence of superficial bladder cancer.Methods:A total of 126 patients with superficial bladder cancer were randomly divided into MMC and pirarubicin group.The MMC group were treated with 30 mg of MMC and 30 ml of 0.9% sodium chloride injection through bladder instillation for 2 hours.The pirarubicin group were treated with 30 mg of pirarubicin and 30 ml of 5% glucose injection through bladder instillation for 30 minutes.The safety and postoperative recurrence of superficial bladder cancer in two groups were observed.Results:All patients were followed up for 24 months.The recurrence rates of MMC and pirarubicin group were 14.3% and 13.7%,the difference of which had no statistical significance and the tumour progression of two groups had no statistical significance(P0.05).The incidence rates of chemical cystitis in MMC and pirarubicin group were 32.0% and 38.5%,the difference of which had no statistical significance(P0.05).The adverse drug reactions of two groups were not obvious.Conclusions:The clinical effects of bladder instillation with MMC and pirarubicin on preventing the postoperative recurrence of superficial bladder cancer are similar.These two drugs can be used as the preferred drug for clinical bladder instillation.
2013, 37(6): 732-734.
Abstract:
Objective:To investigate the association among blood pressure variability,plasma von willebrand factor(vWF)and D-dimer levels in elderly patients with essential hypertension.Methods:One hundred cases whose age over 60 years with essential hypertension(hypertension group)and 100 cases of healthy elderly controls(control group)were randomly recruited into study.The blood pressure variability during 24 hours was monitored and plasma vWF and D-dimer levels were measured for each patient in both groups.The associations between blood pressure variability and plasma vWF and D-dimer levels were analyzed in each group respectively.Results:The blood pressure variability during 24 hours in hypertension group were significantly higher than that in control group(P0.01).The plasma vWF level in hypertension group was significantly higher than that in control group(P0.01).The plasma vWF level was positively correlated with 24 hours systolic blood pressure variability in hypertension group(P0.05).Conclusions:Blood pressure variability in hypertension patients with essential hypertension was increased obviously accompanied with the increase of plasma vWF level,which implying the potential impairment of vascular endothelium.
Objective:To investigate the association among blood pressure variability,plasma von willebrand factor(vWF)and D-dimer levels in elderly patients with essential hypertension.Methods:One hundred cases whose age over 60 years with essential hypertension(hypertension group)and 100 cases of healthy elderly controls(control group)were randomly recruited into study.The blood pressure variability during 24 hours was monitored and plasma vWF and D-dimer levels were measured for each patient in both groups.The associations between blood pressure variability and plasma vWF and D-dimer levels were analyzed in each group respectively.Results:The blood pressure variability during 24 hours in hypertension group were significantly higher than that in control group(P0.01).The plasma vWF level in hypertension group was significantly higher than that in control group(P0.01).The plasma vWF level was positively correlated with 24 hours systolic blood pressure variability in hypertension group(P0.05).Conclusions:Blood pressure variability in hypertension patients with essential hypertension was increased obviously accompanied with the increase of plasma vWF level,which implying the potential impairment of vascular endothelium.
2013, 37(6): 735-737.
Abstract:
Objective:To analyse the influence factors of the floating pregnant women choosing cesarean section(CS)and provide scientific guidance for their health care.Methods:The influence factors of 373 pregnant women choosing CS were investigated by questionnaire.Results:Multiple logistic regression analysis showed that the elderly pregnant women,highly educational level,abortion history and the male newborn were theincreasing choice factors of the CS.Pregnant women tend to CS and thought CS was safe,which were also the increasing choice factors of the CS was(P0.05 to P0.01).Conclusions:To improve the midwifery skills and strengthen the health education for the floating pregnant women can control the excess use of CS.
Objective:To analyse the influence factors of the floating pregnant women choosing cesarean section(CS)and provide scientific guidance for their health care.Methods:The influence factors of 373 pregnant women choosing CS were investigated by questionnaire.Results:Multiple logistic regression analysis showed that the elderly pregnant women,highly educational level,abortion history and the male newborn were theincreasing choice factors of the CS.Pregnant women tend to CS and thought CS was safe,which were also the increasing choice factors of the CS was(P0.05 to P0.01).Conclusions:To improve the midwifery skills and strengthen the health education for the floating pregnant women can control the excess use of CS.
2013, 37(6): 738-740.
Abstract:
Objective:To analyse the clinical characteristics and prevention measures of severe cerebral damage complicated with multiple organ dysfunction syndrome(MODS).Methods:Forty-five patients with severe cerebral damage with 3 to 11 Glasgow coma scores complicated with MODS were treated with control the primary disease and prevention complications.The clinical data of incidence,clinical characteristics and mortality of all patients were retrospectively analyzed.Results:The incidence and pathogenic condition of patients with severe cerebral damage complicated with MODS were high(88.9%)and heavy respectively.Organ dysfunction of pulmonary,stomach and intestine,kidney and heart often happened.The number of organ involved was more than or equal to 3,which mortality was higher than that in patients with 2 organs involved(P0.01).Conclusions:Patients with severe cerebral damage is prone to MODS.The involved organ number is directly related to fatality rate.Treating primary disease,early monitoring organ function,protecting other organ function and prevent multiple organ function failure should be implemented.Comprehensive treatment is a key mean to reduce the mortality rate and improve the prognosis.
Objective:To analyse the clinical characteristics and prevention measures of severe cerebral damage complicated with multiple organ dysfunction syndrome(MODS).Methods:Forty-five patients with severe cerebral damage with 3 to 11 Glasgow coma scores complicated with MODS were treated with control the primary disease and prevention complications.The clinical data of incidence,clinical characteristics and mortality of all patients were retrospectively analyzed.Results:The incidence and pathogenic condition of patients with severe cerebral damage complicated with MODS were high(88.9%)and heavy respectively.Organ dysfunction of pulmonary,stomach and intestine,kidney and heart often happened.The number of organ involved was more than or equal to 3,which mortality was higher than that in patients with 2 organs involved(P0.01).Conclusions:Patients with severe cerebral damage is prone to MODS.The involved organ number is directly related to fatality rate.Treating primary disease,early monitoring organ function,protecting other organ function and prevent multiple organ function failure should be implemented.Comprehensive treatment is a key mean to reduce the mortality rate and improve the prognosis.
2013, 37(6): 753-755.
Abstract:
Objective:To investigate the value of a variety of MrI technologies in diagnosing Budd-Chiari syndrome ( BCS ).Methods:Thirty-two patients with BCS diagnosed by ultrasound and digital subtraction angiography were detected by TSE T1WI/TFE IP,TSE T2WI/SPIr,B-FFE and 3D DCE rA,the MrI of which was analysed.Results:The positive rates of T1WI/TFE IP,TSE T2WI/SPIr,B-FFE and 3D DCE MrA in 32 cases with BCS were 56.7%,59.4%,87.5% and 93.7%,respectively.Conclusions:B-FFE and 3D DCE MrA in diagnosing BCS have high accuracy of location and characterization.The application of a variety of MrI technologies in diagnosing BCS is an ideal mean.
Objective:To investigate the value of a variety of MrI technologies in diagnosing Budd-Chiari syndrome ( BCS ).Methods:Thirty-two patients with BCS diagnosed by ultrasound and digital subtraction angiography were detected by TSE T1WI/TFE IP,TSE T2WI/SPIr,B-FFE and 3D DCE rA,the MrI of which was analysed.Results:The positive rates of T1WI/TFE IP,TSE T2WI/SPIr,B-FFE and 3D DCE MrA in 32 cases with BCS were 56.7%,59.4%,87.5% and 93.7%,respectively.Conclusions:B-FFE and 3D DCE MrA in diagnosing BCS have high accuracy of location and characterization.The application of a variety of MrI technologies in diagnosing BCS is an ideal mean.
2013, 37(6): 756-758.
Abstract:
Objective:To monitor the distribution and drug resistance of clinical isolated common non-fermentative Gram-negative bacilli,so as to provide basis for appropriate use of antibiotics in clinic.Methods:The 728 strains common non-fermentative gram-negative bacilli were collected and their antimicrobial susceptibility was studied.The identification and drug susceptibility tests were detected by paper-disc agar-diffusion method and minimal inhibitory concentration method.The results were evaluated in accordance with the standard of American Clinical Laboratory Information Systems.Results:In the 728 strains non fermentative Gram-negative bacilli,the dominant four strains were Acinetobacter baumannii ( 330 strains ) firstly,Pseudomonas aeruginaosa ( 290 strains ),Stenotrophomonas maltophilia ( 38 strains) and Burkholderia cepacia ( also 38 strains).The main bacterias were most from sputum system,totally 543 strains and the most distribution were detected in intensive care unit ( 308 strains ).The resistant rates of Pseudomonas aeruginosa to imipenem cefoperaxone/sulbactam,piperacillin/tazobactam were all less than 30%.The resistant rates of Acinetobacter baumannii were 31.2% to minocycline,53.94% to cefoperaxone/sulbactam and all lower than others.The resistant rate of Stenotrophomonas maltophilia to minocycline was 5.3%.The resistant rates of Burkholderia cepacia to cotrimoxazole and minocycline were lower than others,21.05% and 15.8% respectively.Conclusions:Different kinds of nonfermentative Gram negative bacilli had a greater difference in drug sensitivity.
Objective:To monitor the distribution and drug resistance of clinical isolated common non-fermentative Gram-negative bacilli,so as to provide basis for appropriate use of antibiotics in clinic.Methods:The 728 strains common non-fermentative gram-negative bacilli were collected and their antimicrobial susceptibility was studied.The identification and drug susceptibility tests were detected by paper-disc agar-diffusion method and minimal inhibitory concentration method.The results were evaluated in accordance with the standard of American Clinical Laboratory Information Systems.Results:In the 728 strains non fermentative Gram-negative bacilli,the dominant four strains were Acinetobacter baumannii ( 330 strains ) firstly,Pseudomonas aeruginaosa ( 290 strains ),Stenotrophomonas maltophilia ( 38 strains) and Burkholderia cepacia ( also 38 strains).The main bacterias were most from sputum system,totally 543 strains and the most distribution were detected in intensive care unit ( 308 strains ).The resistant rates of Pseudomonas aeruginosa to imipenem cefoperaxone/sulbactam,piperacillin/tazobactam were all less than 30%.The resistant rates of Acinetobacter baumannii were 31.2% to minocycline,53.94% to cefoperaxone/sulbactam and all lower than others.The resistant rate of Stenotrophomonas maltophilia to minocycline was 5.3%.The resistant rates of Burkholderia cepacia to cotrimoxazole and minocycline were lower than others,21.05% and 15.8% respectively.Conclusions:Different kinds of nonfermentative Gram negative bacilli had a greater difference in drug sensitivity.
2013, 37(6): 761-764.
Abstract:
Objective:To explore the influence of psychological care involving family members on patients with depression and their caregivers.Methods:One hundred and twenty-four hospitalized patients with depression were randomly divided into observation group and control group received.The observation group received the psychological care involving the family members and the control group received the routine care.All the patients were tested by Hamilton Depression Scale( HAMD) and Hamilton Anxiety Scale( HAMA),and their caregivers were tested by Symptom Checklist( SCL-90) and Family Functional Assessment Scale( FAD) before and the 12th and 24th week afer the intervention.Results:In the 12th and 24th week,the differences in the HAMD,HAMA depression,anxiety,interpersonal sensitivity factor points,SCL-90 total score,behavior control,communication and FAD score between the observation group and the control group were statistically significant( P0.01);There was a positive correlation between the SCL-90 total score and the control factors( P0.05);the SCL 90 total score,anxiety and HAMA factor points had a positive correlation with the total household function( P0.05);The somatization was positively correlated with the communication( P0.05).Conclusions:Psychological care involving family members can greatly enhance the curative effect on patients with depression and relieve their caregivers' depression and anxiety,improving the family function.It is worthy of popularization.
Objective:To explore the influence of psychological care involving family members on patients with depression and their caregivers.Methods:One hundred and twenty-four hospitalized patients with depression were randomly divided into observation group and control group received.The observation group received the psychological care involving the family members and the control group received the routine care.All the patients were tested by Hamilton Depression Scale( HAMD) and Hamilton Anxiety Scale( HAMA),and their caregivers were tested by Symptom Checklist( SCL-90) and Family Functional Assessment Scale( FAD) before and the 12th and 24th week afer the intervention.Results:In the 12th and 24th week,the differences in the HAMD,HAMA depression,anxiety,interpersonal sensitivity factor points,SCL-90 total score,behavior control,communication and FAD score between the observation group and the control group were statistically significant( P0.01);There was a positive correlation between the SCL-90 total score and the control factors( P0.05);the SCL 90 total score,anxiety and HAMA factor points had a positive correlation with the total household function( P0.05);The somatization was positively correlated with the communication( P0.05).Conclusions:Psychological care involving family members can greatly enhance the curative effect on patients with depression and relieve their caregivers' depression and anxiety,improving the family function.It is worthy of popularization.
2013, 37(6): 765-767.
Abstract:
Objective:To explore the curative effect influence of rehabilitation nursing with the core of multiple sensory stimulation onneonatal bilirubin encephalopathy.Methods:Seventy-four neonates with hypoxic-ischemic encephalopathy were randomly divided into control group( 35 cases) and observation group ( 39 cases).Patients in control group were nursed with routine nursing.Patients in observation group were nursed with rehabilitation nursing with the core of multiple sensory stimulation.The developmental quotient( DQ),psychomotor development index( PDI) and mental development index( MDI) of two groups were measured at 6,9 and 12 months before and after treatment.Results:DQ,PDI and MDI of two groups improved significantly at 6,9 and 12 months after treatment( P0.01).Compared with the control group,the DQ of the observation group were significant improvement at 6 and 12 months after treatment( P0.05 to P0.01),and the PDI and MDI were significant improvement at 6,9 and 12 months after treatment( P0.01).Conclusions:The rehabilitation nursing with the core of multiple sensory stimulation can effectively promote the recovery of cranial nerve,motor function and intellectual development of neonatal bilirubin encephalopathy.
Objective:To explore the curative effect influence of rehabilitation nursing with the core of multiple sensory stimulation onneonatal bilirubin encephalopathy.Methods:Seventy-four neonates with hypoxic-ischemic encephalopathy were randomly divided into control group( 35 cases) and observation group ( 39 cases).Patients in control group were nursed with routine nursing.Patients in observation group were nursed with rehabilitation nursing with the core of multiple sensory stimulation.The developmental quotient( DQ),psychomotor development index( PDI) and mental development index( MDI) of two groups were measured at 6,9 and 12 months before and after treatment.Results:DQ,PDI and MDI of two groups improved significantly at 6,9 and 12 months after treatment( P0.01).Compared with the control group,the DQ of the observation group were significant improvement at 6 and 12 months after treatment( P0.05 to P0.01),and the PDI and MDI were significant improvement at 6,9 and 12 months after treatment( P0.01).Conclusions:The rehabilitation nursing with the core of multiple sensory stimulation can effectively promote the recovery of cranial nerve,motor function and intellectual development of neonatal bilirubin encephalopathy.
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