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2013 Vol. 38, No. 8
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2013, 37(8): 925-928.
Abstract:
Objective: To develop the technique for measuring infiltrated macrophage subtypes in injured spinal cord of rats by flow cytometry. Methods: Contusive spinal cord injury( SCI) model in five healthy female SD rats( SCI group) were established by New York University Impactor， other five healthy female SD rats were treated with sham operation( sham operation group) . The injured spinal cords were collected and made into single cell suspension at day 7 after SCI. The infiltrated macrophages were concentrated by density gradient centrifugation using Percoll. The type M1 and M2 macrophages in lesions were identified using CD68 and CD86，or CD163 fluorescent antibodies， and measured by flow cytometry. Results: In rat's spinal cord single cell suspension of the sham operation group，the percentage of CD68 + cells in total cells was( 0. 07 0. 02) %， the percentages of CD86 + and CD163 + cells in CD68 + cells were (9. 90 3. 28) % and( 4. 95 0. 17) %， respectively. In rat's spinal cord single cell suspension of the SCI group， the percentage of CD68 + cells in total cells was( 0. 21 0. 09) %， the percentages of CD86 + and CD163 + cells in CD68 + cells were( 18. 71 0. 49) % and( 27. 07 3. 74) %，respectively. These differences between the sham operation group and SCI group were statistical signficance ( P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: By using the mouse anti-rat CD68，CD86 and CD163 fluorescent antibodies， the infiltrated M1 and M2 macrophage subtypes can be successfully detected by flow cytometry.
Objective: To develop the technique for measuring infiltrated macrophage subtypes in injured spinal cord of rats by flow cytometry. Methods: Contusive spinal cord injury( SCI) model in five healthy female SD rats( SCI group) were established by New York University Impactor， other five healthy female SD rats were treated with sham operation( sham operation group) . The injured spinal cords were collected and made into single cell suspension at day 7 after SCI. The infiltrated macrophages were concentrated by density gradient centrifugation using Percoll. The type M1 and M2 macrophages in lesions were identified using CD68 and CD86，or CD163 fluorescent antibodies， and measured by flow cytometry. Results: In rat's spinal cord single cell suspension of the sham operation group，the percentage of CD68 + cells in total cells was( 0. 07 0. 02) %， the percentages of CD86 + and CD163 + cells in CD68 + cells were (9. 90 3. 28) % and( 4. 95 0. 17) %， respectively. In rat's spinal cord single cell suspension of the SCI group， the percentage of CD68 + cells in total cells was( 0. 21 0. 09) %， the percentages of CD86 + and CD163 + cells in CD68 + cells were( 18. 71 0. 49) % and( 27. 07 3. 74) %，respectively. These differences between the sham operation group and SCI group were statistical signficance ( P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: By using the mouse anti-rat CD68，CD86 and CD163 fluorescent antibodies， the infiltrated M1 and M2 macrophage subtypes can be successfully detected by flow cytometry.
2013, 37(8): 929-931.
Abstract:
Objective: To investigate the expression and clinical significance of cytochrome P4501A1 ( CYP1A1) in squamous cell carcinoma( SCC) . Methods: The expressions of CYP1A1 in normal cervix tissue( 18 cases) ，cervical intraepithelial neoplasia( CIN)tissue( 33 cases) and SCC tissue ( 90 cases) were detected by immunohistochemicalstaining. The relationships between CYP1A1expression and clinicopathological features and prognosis of cervical cancer wereanalyzed. Results: The positive expression rate of CYP1A1 in SCC( 59. 7%) was higher than that in normal cervix(11. 1%) ( P ＜0. 05) . The positive expression rate of CYP1A1 in stage Ⅲ SCC( 69. 8%) was obviously higher than that in stageⅠ( 34. 8%) ( P ＜ 0. 05) . The positive expression rate of CYP1A1 in SCC with lymphnode metastases( 73. 6%) was higher than that in non-lymphnode metastases( 49. 3%) ( P ＜ 0. 05) . The expression differences of CYP1A1 in age，depth of invasion， stage and size of tumor had no statistic sigificance( P ＞ 0. 05) . Conclusions: The high expression of CYP1A1 may be associated with the occurence and development of cervical cancer.
Objective: To investigate the expression and clinical significance of cytochrome P4501A1 ( CYP1A1) in squamous cell carcinoma( SCC) . Methods: The expressions of CYP1A1 in normal cervix tissue( 18 cases) ，cervical intraepithelial neoplasia( CIN)tissue( 33 cases) and SCC tissue ( 90 cases) were detected by immunohistochemicalstaining. The relationships between CYP1A1expression and clinicopathological features and prognosis of cervical cancer wereanalyzed. Results: The positive expression rate of CYP1A1 in SCC( 59. 7%) was higher than that in normal cervix(11. 1%) ( P ＜0. 05) . The positive expression rate of CYP1A1 in stage Ⅲ SCC( 69. 8%) was obviously higher than that in stageⅠ( 34. 8%) ( P ＜ 0. 05) . The positive expression rate of CYP1A1 in SCC with lymphnode metastases( 73. 6%) was higher than that in non-lymphnode metastases( 49. 3%) ( P ＜ 0. 05) . The expression differences of CYP1A1 in age，depth of invasion， stage and size of tumor had no statistic sigificance( P ＞ 0. 05) . Conclusions: The high expression of CYP1A1 may be associated with the occurence and development of cervical cancer.
2013, 37(8): 932-934.
Abstract:
Objective: To observe the effect of Th1 /Th2 type cytokines in pulmonary fibrosis pathogenesis. Methods: Forty male Kunming mice were randomly divided into 3 groups，8 mice in control group were intratracheal injection with saline， and executed on the 7th day; 16 mice in model group were intratracheal injection with bleomycin. Sixteen mice in treatment group were intraperitoneal injection with interferon- and captopril by oral gavage on the day after model-building，4 mice were executed once on the 3rd，7th， 14th and 28th day. Bronchus alveolus lavage fluid( BALF) was collected， the levels of interleukin( IL-4) and IL-12 in BALF were detected by ELISA method. The right lung specimen was collected in control and model groups，and pathological semi-quantitative analysis of alveolitis degree was detected. Results: Compared with control group， IL-12 level in BALF in model group was reduced( P ＜ 0. 01) ; IL-4 level was increased( P ＜ 0. 01) ，Th2 dominant expression was expressed. Compared with model group， IL-12 level in BALF in treatment group was increased( P ＜ 0. 01) ; IL-4 level was reduced( P ＜ 0. 01) ，partly recovered to the dynamic balance of Th1 /Th2. Compared with model group， there was no difference on alveolus inflammation in treatment group at early stage ( 3rd，7th day) ， the degree of alveolus inflammation fibrosis was reduced obviously at later stage( 28th day ) ( P ＜ 0. 01) . Conclusions: Th1 /Th2 cytokine balance disorder is one of mechanism of bleomycin-leading pulmonary fibrosis; combined with interferon- and captopril may partly restore Th1 /Th2 cytokine balance， and lighten the alveolitis ＆ pulmonary fibrosis at the later stage.
Objective: To observe the effect of Th1 /Th2 type cytokines in pulmonary fibrosis pathogenesis. Methods: Forty male Kunming mice were randomly divided into 3 groups，8 mice in control group were intratracheal injection with saline， and executed on the 7th day; 16 mice in model group were intratracheal injection with bleomycin. Sixteen mice in treatment group were intraperitoneal injection with interferon- and captopril by oral gavage on the day after model-building，4 mice were executed once on the 3rd，7th， 14th and 28th day. Bronchus alveolus lavage fluid( BALF) was collected， the levels of interleukin( IL-4) and IL-12 in BALF were detected by ELISA method. The right lung specimen was collected in control and model groups，and pathological semi-quantitative analysis of alveolitis degree was detected. Results: Compared with control group， IL-12 level in BALF in model group was reduced( P ＜ 0. 01) ; IL-4 level was increased( P ＜ 0. 01) ，Th2 dominant expression was expressed. Compared with model group， IL-12 level in BALF in treatment group was increased( P ＜ 0. 01) ; IL-4 level was reduced( P ＜ 0. 01) ，partly recovered to the dynamic balance of Th1 /Th2. Compared with model group， there was no difference on alveolus inflammation in treatment group at early stage ( 3rd，7th day) ， the degree of alveolus inflammation fibrosis was reduced obviously at later stage( 28th day ) ( P ＜ 0. 01) . Conclusions: Th1 /Th2 cytokine balance disorder is one of mechanism of bleomycin-leading pulmonary fibrosis; combined with interferon- and captopril may partly restore Th1 /Th2 cytokine balance， and lighten the alveolitis ＆ pulmonary fibrosis at the later stage.
2013, 37(8): 935-938.
Abstract:
Objective: To explore the effects of lactadherin on promoting the proliferation of T lymphocyte stimulated by dendritic cells( DCs) and cytokines secretion. Methods: Cord blood monocyte was isolated by density gradient centrifugation，and induced and differentiated into DCs. Culture solution was added into the lactadherin experimental group at day 1 and day 5 after isolation. The mixed culture of mature DCs and allogeneic T lymphocytes were implemented. T lymphocyte proliferation were detected using MTT and CFSE， the levels of cytokines were measured using ELISA. Results: Lactadherin could obviously promote the proliferation of T lymphocyte stimulated by DCs( P ＜ 0. 01) ， the result of coculture of DCs and different proportion T lymphocyte was similar，and the ability of Tlymphocyte proliferation was more significant at day 5 after adding lactadherin( P ＜ 0. 01) . DCs induced by lactadherin could promote the level of T cell secreting a variety of cytokines， the level of interferon- increased significantly at the time of adding lactadherin( P ＜0. 05) . Conclusions: lactadherin can promote the proliferation of T lymphocyte stimulated by DCs to activate adaptive immune response.
Objective: To explore the effects of lactadherin on promoting the proliferation of T lymphocyte stimulated by dendritic cells( DCs) and cytokines secretion. Methods: Cord blood monocyte was isolated by density gradient centrifugation，and induced and differentiated into DCs. Culture solution was added into the lactadherin experimental group at day 1 and day 5 after isolation. The mixed culture of mature DCs and allogeneic T lymphocytes were implemented. T lymphocyte proliferation were detected using MTT and CFSE， the levels of cytokines were measured using ELISA. Results: Lactadherin could obviously promote the proliferation of T lymphocyte stimulated by DCs( P ＜ 0. 01) ， the result of coculture of DCs and different proportion T lymphocyte was similar，and the ability of Tlymphocyte proliferation was more significant at day 5 after adding lactadherin( P ＜ 0. 01) . DCs induced by lactadherin could promote the level of T cell secreting a variety of cytokines， the level of interferon- increased significantly at the time of adding lactadherin( P ＜0. 05) . Conclusions: lactadherin can promote the proliferation of T lymphocyte stimulated by DCs to activate adaptive immune response.
2013, 37(8): 939-940,943.
Abstract:
Objective: To explore the effects of edaravone on the levels of serum interleukin-6 ( IL-6) and tumor necrosis factor-( TNF-) in lung injury patients induced by paraquat poisoning. Methods: Forty patients with paraquat poisoning were randomly divided into control group and treatment group. The control group were treated with routine therapy， the treatment group were treated with 30 mg of edaravone twice a day for 14 days combined with routine therapy. The levels of serum IL-6 and TNF- in two groups were measured before and after treatment. Results: The levels of serum IL-6 and TNF- in two groups before treatment were not statistical significance( P ＞ 0. 05) . The levels of serum IL-6 and TNF- in two groups after treatmentobviously decreased( P ＜ 0. 01) . Compared with control group， the decreasing IL-6 and TNF- levels in treatment group aftertreatment were obviously more ( P ＜ 0. 01 ) . Conclusions: Edaravone can effectively decrease the serum levels of IL-6 and TNF- and alleviate the lung injury in patients induced by paraquat poisoning.
Objective: To explore the effects of edaravone on the levels of serum interleukin-6 ( IL-6) and tumor necrosis factor-( TNF-) in lung injury patients induced by paraquat poisoning. Methods: Forty patients with paraquat poisoning were randomly divided into control group and treatment group. The control group were treated with routine therapy， the treatment group were treated with 30 mg of edaravone twice a day for 14 days combined with routine therapy. The levels of serum IL-6 and TNF- in two groups were measured before and after treatment. Results: The levels of serum IL-6 and TNF- in two groups before treatment were not statistical significance( P ＞ 0. 05) . The levels of serum IL-6 and TNF- in two groups after treatmentobviously decreased( P ＜ 0. 01) . Compared with control group， the decreasing IL-6 and TNF- levels in treatment group aftertreatment were obviously more ( P ＜ 0. 01 ) . Conclusions: Edaravone can effectively decrease the serum levels of IL-6 and TNF- and alleviate the lung injury in patients induced by paraquat poisoning.
2013, 37(8): 941-943.
Abstract:
Objective: To explore the application of tubular stomach reconstruction in esophagectomy. Methods: Eighty patients with esophageal carcinoma were devided into tubular stomach group and full stomach group according to different operation Methods( 40 cases each group) . The tubular stomach and whole stomach group were treated with tubular gastroesophagostomy and traditional whole gastroesophagostomy，respectively. The incidence of postoperative pulmonary complications，anastomotic fistula，thoracic stomach syndrome and reflux esophagitis in two groups were observed. Results: No death was found in two groups. The incidence of postoperative pulmonary complications， reflux esophagitis， thoracic stomach syndrome and anastomotic fistula in tubular stomach group and whole stomach group were 17. 5% ＆ 40. 0%，32. 5% ＆ 65. 0%，12. 5% ＆ 32. 5% and 0% ＆ 2. 5%， respectively. The incidence of postoperative thoracic stomach syndrome，pulmonary complications and reflux esophagitis in tubular stomach group were less than those in whole stomach group( P ＜ 0. 05 to P ＜ 0. 01) . The difference of the incidence of anastomotic fistula between two groups was not statistically significant( P ＞ 0. 05) . Conclusions: The tubular stomach reconstruction in esophagectomy is simple and safe，which can improve gastroesophageal reflux， reduce the incidence of adverse gastric emptying and improve postoperative quality of life of patient.
Objective: To explore the application of tubular stomach reconstruction in esophagectomy. Methods: Eighty patients with esophageal carcinoma were devided into tubular stomach group and full stomach group according to different operation Methods( 40 cases each group) . The tubular stomach and whole stomach group were treated with tubular gastroesophagostomy and traditional whole gastroesophagostomy，respectively. The incidence of postoperative pulmonary complications，anastomotic fistula，thoracic stomach syndrome and reflux esophagitis in two groups were observed. Results: No death was found in two groups. The incidence of postoperative pulmonary complications， reflux esophagitis， thoracic stomach syndrome and anastomotic fistula in tubular stomach group and whole stomach group were 17. 5% ＆ 40. 0%，32. 5% ＆ 65. 0%，12. 5% ＆ 32. 5% and 0% ＆ 2. 5%， respectively. The incidence of postoperative thoracic stomach syndrome，pulmonary complications and reflux esophagitis in tubular stomach group were less than those in whole stomach group( P ＜ 0. 05 to P ＜ 0. 01) . The difference of the incidence of anastomotic fistula between two groups was not statistically significant( P ＞ 0. 05) . Conclusions: The tubular stomach reconstruction in esophagectomy is simple and safe，which can improve gastroesophageal reflux， reduce the incidence of adverse gastric emptying and improve postoperative quality of life of patient.
2013, 37(8): 944-945.
Abstract:
Objective: To summarize the effects of tubular anastomat in the digestive tract reconstruction of esophageal resection. Methods: The clinical data of 392 patients with esophageal cancer resection treated with esophagogastric side to side anastomosis using tubular anastomat wereretrospectively analyzed. Results: Anastomotic leakage in 2 cases( 0. 5%) were found， they cured after nutrition support， adequate drainage and anti-infection treatment. Anastomotic stricture in 7 cases ( 1. 7%) were found， they improved after esophagus expansion and stent implanting. Conclusions: The esophagogastric side to side anastomosis using instrument is effective and easy to operate.
Objective: To summarize the effects of tubular anastomat in the digestive tract reconstruction of esophageal resection. Methods: The clinical data of 392 patients with esophageal cancer resection treated with esophagogastric side to side anastomosis using tubular anastomat wereretrospectively analyzed. Results: Anastomotic leakage in 2 cases( 0. 5%) were found， they cured after nutrition support， adequate drainage and anti-infection treatment. Anastomotic stricture in 7 cases ( 1. 7%) were found， they improved after esophagus expansion and stent implanting. Conclusions: The esophagogastric side to side anastomosis using instrument is effective and easy to operate.
2013, 37(8): 946-947,950.
Abstract:
Objective: To compare the clinical efficacy and toxicity of raltitrexed or fluorouracil plus gemcitabine in the treatment of patients with advanced breast cancer. Methods: The curative effects and adverse reactions in 57 cases of advanced breast cancer treated by different therapies were analyzedretrospectively. Group A( 31 cases) were administered raltitrexed plus gemcitabine and group B( 26 cases) fluorouracil plus gemcitabine. Group A were given raltitrexed 3 mg /m2at day 1， and gemcitabine 1 000 mg /m2 at day 1 and day 8; group B were administered fluorouracil 500 mg /m2 from day 1 to day 5，and gemcitabine 1 000 mg /m2 at day 1 and day 8. The therapies were repeated every 21 days for two cycles. Results: The response rates of group A and group B were 38. 71% and 30. 77%， respectively. The difference was not significant between the two groups ( P ＞ 0. 05) . The difference in the occurrence of anorexia，vomiting，diarrhea and transaminase elevation had statistical significance between the two groups( P ＜ 0. 05 to P ＜ 0. 01) ，and the difference in leukopenia was not obvious( P ＞ 0. 05) . Conclusions: The therapy of raltitrexed plus gemcitabine is effective and has sight adverse reactions in treatment of advanced breast cancer， it can be used as one of the regimens for patients with advanced breast cancer.
Objective: To compare the clinical efficacy and toxicity of raltitrexed or fluorouracil plus gemcitabine in the treatment of patients with advanced breast cancer. Methods: The curative effects and adverse reactions in 57 cases of advanced breast cancer treated by different therapies were analyzedretrospectively. Group A( 31 cases) were administered raltitrexed plus gemcitabine and group B( 26 cases) fluorouracil plus gemcitabine. Group A were given raltitrexed 3 mg /m2at day 1， and gemcitabine 1 000 mg /m2 at day 1 and day 8; group B were administered fluorouracil 500 mg /m2 from day 1 to day 5，and gemcitabine 1 000 mg /m2 at day 1 and day 8. The therapies were repeated every 21 days for two cycles. Results: The response rates of group A and group B were 38. 71% and 30. 77%， respectively. The difference was not significant between the two groups ( P ＞ 0. 05) . The difference in the occurrence of anorexia，vomiting，diarrhea and transaminase elevation had statistical significance between the two groups( P ＜ 0. 05 to P ＜ 0. 01) ，and the difference in leukopenia was not obvious( P ＞ 0. 05) . Conclusions: The therapy of raltitrexed plus gemcitabine is effective and has sight adverse reactions in treatment of advanced breast cancer， it can be used as one of the regimens for patients with advanced breast cancer.
2013, 37(8): 948-950.
Abstract:
Objective: To explore the clinical efficacy and safety of hysteroscopic electrotomy in the treatment of submucosal myoma of uterus. Methods: Forty-six patients with submucosal myoma of uterus were divided into hysteroscopic operation group( 26 cases) and laparotomy group ( 20 cases ) . The time of operation，anal exhaust，ambulation and hospital stay，intraoperative blood loss and postoperative fever rate of two groups were analysed. Results: The operative time and intraoperative blood loss of hysteroscopic operation group were shorter or less than those in laparotomy group ( P ＜ 0. 01 ) . The time of anal exhaust，ambulation and hospital stay of hysteroscopic operation group were significantly shorter than those in laparotomy group( P ＜ 0. 01) . The postoperative fever cases in hysteroscopic operation group and that in laparotomy group the difference was not statistical significance( P ＞ 0. 05) . Conclusions:Hysteroscopic electrotomy in the treatment of submucosal myoma of uterus is good clinical efficacy and safety，which can be the first choice of treatment means.
Objective: To explore the clinical efficacy and safety of hysteroscopic electrotomy in the treatment of submucosal myoma of uterus. Methods: Forty-six patients with submucosal myoma of uterus were divided into hysteroscopic operation group( 26 cases) and laparotomy group ( 20 cases ) . The time of operation，anal exhaust，ambulation and hospital stay，intraoperative blood loss and postoperative fever rate of two groups were analysed. Results: The operative time and intraoperative blood loss of hysteroscopic operation group were shorter or less than those in laparotomy group ( P ＜ 0. 01 ) . The time of anal exhaust，ambulation and hospital stay of hysteroscopic operation group were significantly shorter than those in laparotomy group( P ＜ 0. 01) . The postoperative fever cases in hysteroscopic operation group and that in laparotomy group the difference was not statistical significance( P ＞ 0. 05) . Conclusions:Hysteroscopic electrotomy in the treatment of submucosal myoma of uterus is good clinical efficacy and safety，which can be the first choice of treatment means.
2013, 37(8): 951-952,955.
Abstract:
Objective: To evaluate the safety and efficacy of using low-dose of bupivacaine in combined spinal and epiduralanaesthesia( CSEA) in hip surgeries for the elderly. Methods: Sixty elderly patients( ASA physical status Ⅱ to Ⅲ) scheduled for the hip surgery were randomly divided into observation group ( 30 cases，receiving 1 ml of 0. 75% bupivacaine) and control group( 30 cases， receiving 2 ml of 0. 75% bupivacaine) ; CSEA was performed in both groups. The intravenous transfusion and infusion were determined according to the blood pressure and bleeding volume of the patients. The onset time of the sensory block， time to complete block， the highest sensory block level and the number of epidural bupivacaine injections were recorded. The systolic blood pressure， diastolic blood pressure and heart rate before the anesthesia and 5， 15 and 30 minutes after CSEA were observed. Results: There were no significant differences in the onset time of the sensory block， time to complete block and number of epidural bupivacaine injections between the two groups( P ＞ 0. 05) . The changes of the blood pressure of the observation group at 5， 15 and 30 minutes after CSEA were not obvious as compared to those of the control group( P ＜ 0. 05 to P ＜ 0. 01) . The heart rate of the observation group was less affected by anaesthesia at 5 minutes after CSEA than that of the control group( P ＜ 0. 01) . Conclusions: Low-dose of bupivacaine in CSEA may achieve an ideal anesthetic effect and keep a stable hemodynamic status in the elderly patients receving hip surgeries.
Objective: To evaluate the safety and efficacy of using low-dose of bupivacaine in combined spinal and epiduralanaesthesia( CSEA) in hip surgeries for the elderly. Methods: Sixty elderly patients( ASA physical status Ⅱ to Ⅲ) scheduled for the hip surgery were randomly divided into observation group ( 30 cases，receiving 1 ml of 0. 75% bupivacaine) and control group( 30 cases， receiving 2 ml of 0. 75% bupivacaine) ; CSEA was performed in both groups. The intravenous transfusion and infusion were determined according to the blood pressure and bleeding volume of the patients. The onset time of the sensory block， time to complete block， the highest sensory block level and the number of epidural bupivacaine injections were recorded. The systolic blood pressure， diastolic blood pressure and heart rate before the anesthesia and 5， 15 and 30 minutes after CSEA were observed. Results: There were no significant differences in the onset time of the sensory block， time to complete block and number of epidural bupivacaine injections between the two groups( P ＞ 0. 05) . The changes of the blood pressure of the observation group at 5， 15 and 30 minutes after CSEA were not obvious as compared to those of the control group( P ＜ 0. 05 to P ＜ 0. 01) . The heart rate of the observation group was less affected by anaesthesia at 5 minutes after CSEA than that of the control group( P ＜ 0. 01) . Conclusions: Low-dose of bupivacaine in CSEA may achieve an ideal anesthetic effect and keep a stable hemodynamic status in the elderly patients receving hip surgeries.
2013, 37(8): 953-955.
Abstract:
Objective: To explore the surgical method and clinical effect of laparoscopic colorectal-carcinoma surgery. Methods:Fourty-eight cases with colorectal cancer were treated with laparoscopic technique according to the principle of open operation，which included right colon resection in 5 cases， left colon resection in 2 cases， sigmoid colon resection in 4 cases， rectal anterior resection in 20 cases and perineum combined with rectum resection in 17 cases. Results: In 48 cases，1 case with upper rectal carcinoma invasing ureter was converted to open operation. Postoperative recurrent hemafecia in 1 case treated with right colon resection was found，who was stopped bleeding by suturing anastomotic stoma at day 3 after operation， and cured. The time of operation and postoperative intestinal peristalsis recovery were 100 to 190 minutes and 1 to 4 days， respectively. The intraoperative blood loss was 40 to 250 ml. Anastomotic leakage and postoperative urinary retention were not found. Postoperative hospital stay was 7 to 12 days. Fourty-six cases were followed up for 1 to 27 months，1 cases with peritoneal metastases was found in 8 months after operation and 2 patients discharged after 6 months restored after conservative treatment in hospital once again because of adhesive ileus. Conclusions: Laparoscopic colorectal-carcinoma surgery is safe， less trauma， less pain and quick recovery. Skilled laparoscopic technology and rich experience in open colorectal surgery for doctor is the key to the success of operation.
Objective: To explore the surgical method and clinical effect of laparoscopic colorectal-carcinoma surgery. Methods:Fourty-eight cases with colorectal cancer were treated with laparoscopic technique according to the principle of open operation，which included right colon resection in 5 cases， left colon resection in 2 cases， sigmoid colon resection in 4 cases， rectal anterior resection in 20 cases and perineum combined with rectum resection in 17 cases. Results: In 48 cases，1 case with upper rectal carcinoma invasing ureter was converted to open operation. Postoperative recurrent hemafecia in 1 case treated with right colon resection was found，who was stopped bleeding by suturing anastomotic stoma at day 3 after operation， and cured. The time of operation and postoperative intestinal peristalsis recovery were 100 to 190 minutes and 1 to 4 days， respectively. The intraoperative blood loss was 40 to 250 ml. Anastomotic leakage and postoperative urinary retention were not found. Postoperative hospital stay was 7 to 12 days. Fourty-six cases were followed up for 1 to 27 months，1 cases with peritoneal metastases was found in 8 months after operation and 2 patients discharged after 6 months restored after conservative treatment in hospital once again because of adhesive ileus. Conclusions: Laparoscopic colorectal-carcinoma surgery is safe， less trauma， less pain and quick recovery. Skilled laparoscopic technology and rich experience in open colorectal surgery for doctor is the key to the success of operation.
2013, 37(8): 956-958,961.
Abstract:
Objective: To investigate the clinical efficacy and safety of minimally invasive percutaneous nephrolithotomy( MPCNL) and standard percutaneous nephrolithotomy( PCNL) in the treatment of complex renal calculi. Methods: The clinical data of 96 patients with complex renal calculi who had been treated by PCNL or MPCNL were analyzedretrospectively. Among the 51 cases in the MPCNL group， 23 had staghorn calculi and 28 multiple calculi; while in the PCNL group， 21 had staghorn calculi and 24 multiple calculi. The operation time，blood transfusion rate， calculus clearance rate， complications and postoperative hospital day were compared between the two groups. Results: The average operation time in the MPCNL group was significantly longer than that in the PCNL group( P ＜ 0. 01) . There was no statistical difference in the calculus clearance rate，blood transfusion rate，complications or postoperative hospital day between the two groups( P ＞ 0. 05) . Conclusions: Both MPCNL and PCNL have the advantages of high calculus clearance rate，quick recovery， short hospital day and few complications. The clinical efficacy and safety of MPCNL and PCNL are not significantly different.
Objective: To investigate the clinical efficacy and safety of minimally invasive percutaneous nephrolithotomy( MPCNL) and standard percutaneous nephrolithotomy( PCNL) in the treatment of complex renal calculi. Methods: The clinical data of 96 patients with complex renal calculi who had been treated by PCNL or MPCNL were analyzedretrospectively. Among the 51 cases in the MPCNL group， 23 had staghorn calculi and 28 multiple calculi; while in the PCNL group， 21 had staghorn calculi and 24 multiple calculi. The operation time，blood transfusion rate， calculus clearance rate， complications and postoperative hospital day were compared between the two groups. Results: The average operation time in the MPCNL group was significantly longer than that in the PCNL group( P ＜ 0. 01) . There was no statistical difference in the calculus clearance rate，blood transfusion rate，complications or postoperative hospital day between the two groups( P ＞ 0. 05) . Conclusions: Both MPCNL and PCNL have the advantages of high calculus clearance rate，quick recovery， short hospital day and few complications. The clinical efficacy and safety of MPCNL and PCNL are not significantly different.
2013, 37(8): 959-961.
Abstract:
Objective: To analyse the risk factors of deaths with acute cerebrovascular disease treated with mechanical ventilation. Methods: Eight-one patients with acute cerebrovascular disease treated with mechanical ventilation were divided into survival group and death group according to the hospital death. The clinical data of all patients were statistically analysed. Results: The differences of activated partial thromboplastin time，blood glucose， chronic health evaluation， incision of trachea， lung infection， infratentorial lesions，hemorrhage injury，hospitalization time，ICU length of stay and mechanical ventilation time between two groups were statistical significance( P ＜ 0. 05 － P ＜ 0. 01) . The duration of mechanical ventilation，blood glucose， infratentorial lesions， lung infection were independent risk factors of death，hospitalization time was protective factors of death( P ＜ 0. 05 － P ＜ 0. 01) . Conclusions: The patients with long mechanical ventilation time，high blood sugar， infratentorial lesions and lung infection should be closely monitored and treated correspondingly，which can reduce the mortality rate of patients and improve patients prognosis.
Objective: To analyse the risk factors of deaths with acute cerebrovascular disease treated with mechanical ventilation. Methods: Eight-one patients with acute cerebrovascular disease treated with mechanical ventilation were divided into survival group and death group according to the hospital death. The clinical data of all patients were statistically analysed. Results: The differences of activated partial thromboplastin time，blood glucose， chronic health evaluation， incision of trachea， lung infection， infratentorial lesions，hemorrhage injury，hospitalization time，ICU length of stay and mechanical ventilation time between two groups were statistical significance( P ＜ 0. 05 － P ＜ 0. 01) . The duration of mechanical ventilation，blood glucose， infratentorial lesions， lung infection were independent risk factors of death，hospitalization time was protective factors of death( P ＜ 0. 05 － P ＜ 0. 01) . Conclusions: The patients with long mechanical ventilation time，high blood sugar， infratentorial lesions and lung infection should be closely monitored and treated correspondingly，which can reduce the mortality rate of patients and improve patients prognosis.
The effect of simvastatin in the treatment of pulmonary heart disease complicated with heart failure
2013, 37(8): 962-963,966.
Abstract:
Objective: To investigate the effects of simvastatin in the treatment of pulmonary heart disease with heart failure. Methods: Ninety-six patients with pulmonary heart disease complicated with heart failure were randomly divided into observation group and control group( 48 cases each group) . The control group were treated with conventional means， the observation group were treated with simvastatin based on the conventional means. The therapeutic effect，blood gas index and pulmonary artery pressure index of two groups were observed. Results: The blood gas and pulmonary artery pressure indexes of the observation group after treatmet were significantly better than those in control group( P ＜ 0. 01) . The total effective rates of the observation group and control group were 95. 83% and 75. 00%， respectively， the difference of which was statistical significance ( P ＜ 0. 05 ) . Conclusions: Simvastatin can improve the heart function in patients with pulmonary heart disease complicated with heart failure and treatment effects，which has certain clinic application value.
Objective: To investigate the effects of simvastatin in the treatment of pulmonary heart disease with heart failure. Methods: Ninety-six patients with pulmonary heart disease complicated with heart failure were randomly divided into observation group and control group( 48 cases each group) . The control group were treated with conventional means， the observation group were treated with simvastatin based on the conventional means. The therapeutic effect，blood gas index and pulmonary artery pressure index of two groups were observed. Results: The blood gas and pulmonary artery pressure indexes of the observation group after treatmet were significantly better than those in control group( P ＜ 0. 01) . The total effective rates of the observation group and control group were 95. 83% and 75. 00%， respectively， the difference of which was statistical significance ( P ＜ 0. 05 ) . Conclusions: Simvastatin can improve the heart function in patients with pulmonary heart disease complicated with heart failure and treatment effects，which has certain clinic application value.
2013, 37(8): 964-966.
Abstract:
Objective: To evaluate the clinical efficacy and safety of Prolift anterior pelvic floor reconstruction combined with vaginal posterior Gynemesh in the treatment of stage Ⅱto Ⅲ pelvic organ prolapse( POP) . Methods: Twenty-five patients with stage Ⅱto Ⅲ pelvic organ prolapse rated by POP quantification( POP-Q) were treated with Prolift anterior pelvic floor reconstruction combined with vaginal posterior Gynemesh. The changes of the indicators and complications of all patients were analysed by subjective assessment ( according to pelvic organ prolapse quality of life score) and Objective assessment ( according to POP-Q examination score) in perioperative and postoperative period. Results: The operation time and blood loss amount were ranging from 30 to 60minutes and ranging from 30 to 90 ml， respectively. No surrounding organ damage and massive hemorrhage were found. The mean hospital stays time and median following up time were ranging from 3 to 6 days and ranging from 1 to 12 months， respectively. No vaginal wall and uterine prolapse were found at 4 weeks after the operation. The postoperation indicators of POP-Q and the score of pelvic floor function were lower than those in preoperation( P ＜0. 01) . Conclusions: Prolift anterior pelvic floor reconstruction combined with vaginal posterior Gynemesh is few complications， light pain， qucik recovery， economy and safe. Its short-term curative effects are good， and long-term effects are uncertain.
Objective: To evaluate the clinical efficacy and safety of Prolift anterior pelvic floor reconstruction combined with vaginal posterior Gynemesh in the treatment of stage Ⅱto Ⅲ pelvic organ prolapse( POP) . Methods: Twenty-five patients with stage Ⅱto Ⅲ pelvic organ prolapse rated by POP quantification( POP-Q) were treated with Prolift anterior pelvic floor reconstruction combined with vaginal posterior Gynemesh. The changes of the indicators and complications of all patients were analysed by subjective assessment ( according to pelvic organ prolapse quality of life score) and Objective assessment ( according to POP-Q examination score) in perioperative and postoperative period. Results: The operation time and blood loss amount were ranging from 30 to 60minutes and ranging from 30 to 90 ml， respectively. No surrounding organ damage and massive hemorrhage were found. The mean hospital stays time and median following up time were ranging from 3 to 6 days and ranging from 1 to 12 months， respectively. No vaginal wall and uterine prolapse were found at 4 weeks after the operation. The postoperation indicators of POP-Q and the score of pelvic floor function were lower than those in preoperation( P ＜0. 01) . Conclusions: Prolift anterior pelvic floor reconstruction combined with vaginal posterior Gynemesh is few complications， light pain， qucik recovery， economy and safe. Its short-term curative effects are good， and long-term effects are uncertain.
2013, 37(8): 967-969.
Abstract:
Objective: To investigate the relationship between the uric acid and cardiovascular diseases( CVD) in patients receiving maintenance hemodialysis， and the risk factors for CVD. Methods: Ninety-seven maintenance hemodialysis patients were divided into CVD group and non-CVD group. The clinical data were collectedretrospectively and analyzed with univariate and multivariate models to find out the risk factors for CVD. Results: The level of serum uric acid in CVD group was significantly higher than that in non-CVD group( P ＜ 0. 01) ， and the duration of dialysis， fasting plasma glucose，mean arterial blood pressure( MBP) and total cholesterol( TC)levels were significantly different between the two groups( P ＜ 0. 01) ; logistic regression analysis showed that the uric acid，MBP and TC level were positively correlated with the incidence of CVD( P ＜ 0. 05 － P ＜ 0. 01) . Conclusions: In maintenance hemodialysis patients，the serum uric acid，MBP and TC levels are independent risk factors for CVD.
Objective: To investigate the relationship between the uric acid and cardiovascular diseases( CVD) in patients receiving maintenance hemodialysis， and the risk factors for CVD. Methods: Ninety-seven maintenance hemodialysis patients were divided into CVD group and non-CVD group. The clinical data were collectedretrospectively and analyzed with univariate and multivariate models to find out the risk factors for CVD. Results: The level of serum uric acid in CVD group was significantly higher than that in non-CVD group( P ＜ 0. 01) ， and the duration of dialysis， fasting plasma glucose，mean arterial blood pressure( MBP) and total cholesterol( TC)levels were significantly different between the two groups( P ＜ 0. 01) ; logistic regression analysis showed that the uric acid，MBP and TC level were positively correlated with the incidence of CVD( P ＜ 0. 05 － P ＜ 0. 01) . Conclusions: In maintenance hemodialysis patients，the serum uric acid，MBP and TC levels are independent risk factors for CVD.
2013, 37(8): 969-971.
Abstract:
Objective: To investigate the effects of ureteroscope holmium laser lithotripsy( UrL) and percutaneous nephrolithotripsy( PCNL) in management of upper urinary tract calculi. Methods: Forty-three patients with upper ureter calculi were included in the study. Twenty-four of them were treated by UrL( UrL group) and 19 by PCNL( PCNL group) . The operation time，post-operativehospital stay，post-operative infection， stone-free rate and complication were compared between the two groups. Results: The operation time and hospital stay of the UrL group were significantly shorter than those of the PCNL group( P ＜ 0. 01) . The stone-free rate，postoperativeinfection and complication had no significant differences between the two groups( P ＞ 0. 05) . Conclusions: UrL is the bestchoice for patients whose ureteric calculi are located in a low position and have little hydronephrosis， or the patients who cannot undergogeneral anesthesia; PCNL is more suitable for patients whose ureteric calculi are in the upper part or near the pelvis with obvious hydronephrosis.
Objective: To investigate the effects of ureteroscope holmium laser lithotripsy( UrL) and percutaneous nephrolithotripsy( PCNL) in management of upper urinary tract calculi. Methods: Forty-three patients with upper ureter calculi were included in the study. Twenty-four of them were treated by UrL( UrL group) and 19 by PCNL( PCNL group) . The operation time，post-operativehospital stay，post-operative infection， stone-free rate and complication were compared between the two groups. Results: The operation time and hospital stay of the UrL group were significantly shorter than those of the PCNL group( P ＜ 0. 01) . The stone-free rate，postoperativeinfection and complication had no significant differences between the two groups( P ＞ 0. 05) . Conclusions: UrL is the bestchoice for patients whose ureteric calculi are located in a low position and have little hydronephrosis， or the patients who cannot undergogeneral anesthesia; PCNL is more suitable for patients whose ureteric calculi are in the upper part or near the pelvis with obvious hydronephrosis.
2013, 37(8): 972-974.
Abstract:
Objective: To compare the effects of the minimally invasive trepanation and drainage and the small bone window craniotomy in the treatment of moderate amounts of hypertensive cerebral hemorrhage in basal ganglia. Methods: Forty-eight patients with moderate amounts of hypertensive cerebral hemorrhage in basal ganglia were treated with minimally invasive trepanation and drainage( trepanation and drainage group，25 cases) and small bone window craniotomy ( small bone window group，23 cases) ，respectively. Their clinical data wereretrospectively analyzed. Results: The time of operation and hospital stays and peri-operative bleeding in trepanation and drainage group were significantly lower than those in small bone window group( P ＜ 0. 05 to P ＜ 0. 01) . The differences of the overall incidence of complications，curative effects and good long-term prognosis rate between two groups had no statistical significance( P ＞ 0. 05) . Conclusions: The curative effects of the minimally invasive trepanation and drainage and the small bone window craniotomy in the treatment of moderate amounts of hypertensive cerebral hemorrhage in basal ganglia are good. The time of operation and hospital stays and peri-operative bleeding in trepanation and drainage group are lower than those in small bone window group.
Objective: To compare the effects of the minimally invasive trepanation and drainage and the small bone window craniotomy in the treatment of moderate amounts of hypertensive cerebral hemorrhage in basal ganglia. Methods: Forty-eight patients with moderate amounts of hypertensive cerebral hemorrhage in basal ganglia were treated with minimally invasive trepanation and drainage( trepanation and drainage group，25 cases) and small bone window craniotomy ( small bone window group，23 cases) ，respectively. Their clinical data wereretrospectively analyzed. Results: The time of operation and hospital stays and peri-operative bleeding in trepanation and drainage group were significantly lower than those in small bone window group( P ＜ 0. 05 to P ＜ 0. 01) . The differences of the overall incidence of complications，curative effects and good long-term prognosis rate between two groups had no statistical significance( P ＞ 0. 05) . Conclusions: The curative effects of the minimally invasive trepanation and drainage and the small bone window craniotomy in the treatment of moderate amounts of hypertensive cerebral hemorrhage in basal ganglia are good. The time of operation and hospital stays and peri-operative bleeding in trepanation and drainage group are lower than those in small bone window group.
2013, 37(8): 975-976,979.
Abstract:
Objective: To investigate the safety and feasibility of the postoperative primary suture of laparoscopic common bile duct exploration( LCBDE) . Methods: Eighty-two patients with choledocholithiasis treated with LCBDE were divided into primary suture group( 42 cases) and T tube drainage group ( 40 cases) . Results: The differences of operative time，blood loss and postoperative complications between two groups were not statistical significance ( P ＞ 0. 05 ) . The time of the first anus exhaust，postoperative ambulation and hospital stay in primary suture group were ( 24. 93 3. 29) hours，( 2. 39 1. 06) days and ( 6. 5 0. 51) days，respectively，which was significantly less than those in T-tube group( P ＜ 0. 01) . Conclusions: The postoperative selective primary suture of LCBDE is safe and feasible，which can significantly shorten the recovery time， promote early recovery and be worth being further popularized
Objective: To investigate the safety and feasibility of the postoperative primary suture of laparoscopic common bile duct exploration( LCBDE) . Methods: Eighty-two patients with choledocholithiasis treated with LCBDE were divided into primary suture group( 42 cases) and T tube drainage group ( 40 cases) . Results: The differences of operative time，blood loss and postoperative complications between two groups were not statistical significance ( P ＞ 0. 05 ) . The time of the first anus exhaust，postoperative ambulation and hospital stay in primary suture group were ( 24. 93 3. 29) hours，( 2. 39 1. 06) days and ( 6. 5 0. 51) days，respectively，which was significantly less than those in T-tube group( P ＜ 0. 01) . Conclusions: The postoperative selective primary suture of LCBDE is safe and feasible，which can significantly shorten the recovery time， promote early recovery and be worth being further popularized
2013, 37(8): 977-979.
Abstract:
Objective: To compare the clinical effects of proximal femoral nail( PFN) and proximal femoral locking plate( PFLP) in the treatment of the femoral intertrochanteric fractures. Methods: Seventy-one patients with femoral intertrochanteric fractures were divided into PFN group( 39 cases) and PFLP group( 32 cases) according to their different internal fixation Methods. According to Evans classification， there were 31 cases of type Ⅰ，Ⅱ fractures and 40 cases of type Ⅲ to Ⅴ fractures. The operative time，blood loss，bone healing time， complications and Harris Hip Score of two groups were compared. Results: All cases were primary healing， and followed up for 9 to 14 months. The differences of the blood loss，bone healing time and Harris Hip Score of patients with type Ⅰ，Ⅱ fractures in two groups were not statistical significance( P ＞ 0. 05) . The blood loss of patients with type Ⅲ to Ⅴ fractures in PFN group was significantly lower than that in PFLP group( P ＜ 0. 01) . Conclusions: The effects of PFN and PFLP in the treatment of type Ⅰ，Ⅱ fractures have no significant difference， the effect of PFN in the treatment of type Ⅲ to Ⅴ fractures are better than that of PFLP.
Objective: To compare the clinical effects of proximal femoral nail( PFN) and proximal femoral locking plate( PFLP) in the treatment of the femoral intertrochanteric fractures. Methods: Seventy-one patients with femoral intertrochanteric fractures were divided into PFN group( 39 cases) and PFLP group( 32 cases) according to their different internal fixation Methods. According to Evans classification， there were 31 cases of type Ⅰ，Ⅱ fractures and 40 cases of type Ⅲ to Ⅴ fractures. The operative time，blood loss，bone healing time， complications and Harris Hip Score of two groups were compared. Results: All cases were primary healing， and followed up for 9 to 14 months. The differences of the blood loss，bone healing time and Harris Hip Score of patients with type Ⅰ，Ⅱ fractures in two groups were not statistical significance( P ＞ 0. 05) . The blood loss of patients with type Ⅲ to Ⅴ fractures in PFN group was significantly lower than that in PFLP group( P ＜ 0. 01) . Conclusions: The effects of PFN and PFLP in the treatment of type Ⅰ，Ⅱ fractures have no significant difference， the effect of PFN in the treatment of type Ⅲ to Ⅴ fractures are better than that of PFLP.
2013, 37(8): 980-982.
Abstract:
Objective: To investigate the influencing prognostic factors of cerebral hemorrhage. Methods: One hundred and sixty caseswith cerebral hemorrhage were divided into death group( 20 cases ) and survival group( 140cases) according to 21 days survival and death. The clinical data of 160 cases wereretrospectively analysed. Results: rates of massive haemorrhage， disturbance of consciousness，brainstem hemorrhage and the incidence of abnormal serum sodium in death group were higher than that in survival group，the difference was statistically significant( P ＜ 0. 01) . Conclusions: The prognosis of cerebral hemorrhage is correlated with massive hemorrhage， consciousness disorders，blood sodium disorders， and brain stem hemorrhage.
Objective: To investigate the influencing prognostic factors of cerebral hemorrhage. Methods: One hundred and sixty caseswith cerebral hemorrhage were divided into death group( 20 cases ) and survival group( 140cases) according to 21 days survival and death. The clinical data of 160 cases wereretrospectively analysed. Results: rates of massive haemorrhage， disturbance of consciousness，brainstem hemorrhage and the incidence of abnormal serum sodium in death group were higher than that in survival group，the difference was statistically significant( P ＜ 0. 01) . Conclusions: The prognosis of cerebral hemorrhage is correlated with massive hemorrhage， consciousness disorders，blood sodium disorders， and brain stem hemorrhage.
2013, 37(8): 982-984.
Abstract:
Objective: To explore the effects of propofol and sevoflurane anesthesia on postoperatively cognitive function of aged patients in orthopedics. Methods: One hundred and twenty aged patients undergone orthopedic surgery were randomly divided into propofol and sevoflurane group( 60 cases， each group) . The propofol and sevoflurane group were anesthetized by injection 1 to 3 g /ml of propofol and inhalation 1% to 3% of sevoflurane after anesthesia induction，respectively. The serum levels of S100 and cognitive function in two groups were compared before and after operation. Results: Compared with preoperation， the scores of cognitive function in the propofol group decreased at 1，3 and 6 hours after operation( P ＜ 0. 01) ， the difference of cognitive function score beteween postoperative 1 hour and before operation has no statistic significance( P ＞ 0. 05) ，but its score at postoperative 6 days was higher( P ＜ 0. 01) . Compared with preoperation， the scores of cognitive function in the sevoflurane group decreased at 1，3，6， 24 and 72 hours after operation( P ＜ 0. 01) ， the differences of cognitive function score beteween postoperative 1 and 72 hours and before operation have no statistic significance( P ＞ 0. 05) . The scores of cognitive function in the sevoflurane group were lower than those in propofol group at 1，3，6， 24 and 72 hours after operation( P＜0. 01) . Compared with preopration， the postoperative serum levels of S100 protein in two groups were significant higher( P ＜0. 01)， the level of S100 in the sevoflurane group was significantly higher than those in propofol group( P ＜0. 01) . Conclusions: Both propofol and sevoflurane affect the patient's cognitive function， the effect of sevoflurane is the most.
Objective: To explore the effects of propofol and sevoflurane anesthesia on postoperatively cognitive function of aged patients in orthopedics. Methods: One hundred and twenty aged patients undergone orthopedic surgery were randomly divided into propofol and sevoflurane group( 60 cases， each group) . The propofol and sevoflurane group were anesthetized by injection 1 to 3 g /ml of propofol and inhalation 1% to 3% of sevoflurane after anesthesia induction，respectively. The serum levels of S100 and cognitive function in two groups were compared before and after operation. Results: Compared with preoperation， the scores of cognitive function in the propofol group decreased at 1，3 and 6 hours after operation( P ＜ 0. 01) ， the difference of cognitive function score beteween postoperative 1 hour and before operation has no statistic significance( P ＞ 0. 05) ，but its score at postoperative 6 days was higher( P ＜ 0. 01) . Compared with preoperation， the scores of cognitive function in the sevoflurane group decreased at 1，3，6， 24 and 72 hours after operation( P ＜ 0. 01) ， the differences of cognitive function score beteween postoperative 1 and 72 hours and before operation have no statistic significance( P ＞ 0. 05) . The scores of cognitive function in the sevoflurane group were lower than those in propofol group at 1，3，6， 24 and 72 hours after operation( P＜0. 01) . Compared with preopration， the postoperative serum levels of S100 protein in two groups were significant higher( P ＜0. 01)， the level of S100 in the sevoflurane group was significantly higher than those in propofol group( P ＜0. 01) . Conclusions: Both propofol and sevoflurane affect the patient's cognitive function， the effect of sevoflurane is the most.
2013, 37(8): 985-986,989.
Abstract:
To explore the curative effects of preoperative concurrent chemoradiotherapy for Ⅱb cervical cancer. Methods:Fifty-eight cases with Ⅱb cervical cancer were divided into group A( 28 cases) and group B( 30 cases) . Group A and B were treated with chemotherapy and concurrent chemoradiotherapy before operation， respectively. The clinical effects，adverse reactions，operative time，blood loss，pathological features and recovery time of bladder function in two groups were analyzed. Results: The clinical effective rate in group B( 90. 00%) was higher than that in group A( 67. 86%) ( P ＜ 0. 05) . The differences of operative time，blood loss and recovery time of bladder function in two groups were not statistical significante( P ＞ 0. 05) . The preoperative chemoradiotherapy could decrease parametrial invasion and pelvic lymphnode metastasis ( P ＜ 0. 05). Conclusions: The preoperative concurrent chemoradiotherapy for Ⅱb cervical cancer can improve the clinical effects and resectable rate and decrease parametrial invasion and pelvic lymphnode metastasis，which is a practicable means.
To explore the curative effects of preoperative concurrent chemoradiotherapy for Ⅱb cervical cancer. Methods:Fifty-eight cases with Ⅱb cervical cancer were divided into group A( 28 cases) and group B( 30 cases) . Group A and B were treated with chemotherapy and concurrent chemoradiotherapy before operation， respectively. The clinical effects，adverse reactions，operative time，blood loss，pathological features and recovery time of bladder function in two groups were analyzed. Results: The clinical effective rate in group B( 90. 00%) was higher than that in group A( 67. 86%) ( P ＜ 0. 05) . The differences of operative time，blood loss and recovery time of bladder function in two groups were not statistical significante( P ＞ 0. 05) . The preoperative chemoradiotherapy could decrease parametrial invasion and pelvic lymphnode metastasis ( P ＜ 0. 05). Conclusions: The preoperative concurrent chemoradiotherapy for Ⅱb cervical cancer can improve the clinical effects and resectable rate and decrease parametrial invasion and pelvic lymphnode metastasis，which is a practicable means.
2013, 37(8): 987-989.
Abstract:
Objective: To explore the effects of Qingyi decoction combined with enteral nutrition( EN) in the sequential treatment of severe acute pancreatitis( SAP) . Methods: Forty-one patients with SAP were randomly divided into treatment group( 21 cases) and control group( 20 cases) . The control group were treated with routine method combined with parenteral nutrition， the treatment group were sequentially treated with Qingyi decoction combined with enteral nutrition. The abdominal symptoms and signs， time of intestinal function recovery， incidence of peripancreatic abscess /pseudocyst， fatality rate and hospital stays in two groups were recorded. The acute physiology and chronic health evaluation Ⅱ score，serum albumin concentration and CT scan of upper abdominal were investigated within 24 hours of admission and at 7th day，14th day after treatment. Results: The time of intestinal function recovery and WBCrestore， and hospital stays in treatment group were less than those in the control group( P ＜ 0. 01) . At 7 th and 14th day after treatment，the acute physiology and chronic health evaluation Ⅱ score and CT severity index in treatment group were lower than those in control group( P ＜ 0. 05 － P ＜ 0. 01) ，but its serum albumin concentration was high( P ＜ 0. 01) . Conclusions: The sequential therapy of Qingyi decoction combined with enteral nutrition can significantly promote the disease recovery，short hospital stays and improve patient prognosis，which has better clinical application value.
Objective: To explore the effects of Qingyi decoction combined with enteral nutrition( EN) in the sequential treatment of severe acute pancreatitis( SAP) . Methods: Forty-one patients with SAP were randomly divided into treatment group( 21 cases) and control group( 20 cases) . The control group were treated with routine method combined with parenteral nutrition， the treatment group were sequentially treated with Qingyi decoction combined with enteral nutrition. The abdominal symptoms and signs， time of intestinal function recovery， incidence of peripancreatic abscess /pseudocyst， fatality rate and hospital stays in two groups were recorded. The acute physiology and chronic health evaluation Ⅱ score，serum albumin concentration and CT scan of upper abdominal were investigated within 24 hours of admission and at 7th day，14th day after treatment. Results: The time of intestinal function recovery and WBCrestore， and hospital stays in treatment group were less than those in the control group( P ＜ 0. 01) . At 7 th and 14th day after treatment，the acute physiology and chronic health evaluation Ⅱ score and CT severity index in treatment group were lower than those in control group( P ＜ 0. 05 － P ＜ 0. 01) ，but its serum albumin concentration was high( P ＜ 0. 01) . Conclusions: The sequential therapy of Qingyi decoction combined with enteral nutrition can significantly promote the disease recovery，short hospital stays and improve patient prognosis，which has better clinical application value.
2013, 37(8): 990-991,994.
Abstract:
Objective: To observe the effect of the percutaneous vertebroplasty ( PVP) and percutaneous kyphoplasty ( PKP) for osteoporotic vertebral compression fractures in patients. Methods: Thirty-five patients were treated with PVP and 12 patients were treated with PKP. AS scores，SF-36 score，vertebral body height，kyphosis correction rate ofCobb' s angle were compared in the two groups. Results: VAS and SF-36 scores were improved significantly in both two groups ( P ＜ 0. 01) . But there was no statistically significant difference before and after the surgery in the two groups( P ＞ 0. 05) . Vertebral body height restoration in PKP group was obviously better than that in PVP group( P ＜ 0. 01) ， and in kyphosis correction rate Cobb's angle， the two groups were no significant difference( P ＞ 0. 05) . Conclusions: PVP and PKP both have advantages and disadvantages， so according to the actual situation of the patients and then consider use PKP or PVP.
Objective: To observe the effect of the percutaneous vertebroplasty ( PVP) and percutaneous kyphoplasty ( PKP) for osteoporotic vertebral compression fractures in patients. Methods: Thirty-five patients were treated with PVP and 12 patients were treated with PKP. AS scores，SF-36 score，vertebral body height，kyphosis correction rate ofCobb' s angle were compared in the two groups. Results: VAS and SF-36 scores were improved significantly in both two groups ( P ＜ 0. 01) . But there was no statistically significant difference before and after the surgery in the two groups( P ＞ 0. 05) . Vertebral body height restoration in PKP group was obviously better than that in PVP group( P ＜ 0. 01) ， and in kyphosis correction rate Cobb's angle， the two groups were no significant difference( P ＞ 0. 05) . Conclusions: PVP and PKP both have advantages and disadvantages， so according to the actual situation of the patients and then consider use PKP or PVP.
2013, 37(8): 992-994.
Abstract:
Objective: To investigate the expression of Bcl-2 and CD34 in the head，neck and body part of the primary pterygium， and to explore the pathogenesis of pterygium. Methods: The expressions of Bcl-2 and CD34 in 30 specimens from patients with primary pterygium and 5 specimens of normal bulbar conjunctiva were detected by immunohistochemical staining ( EliVision method) ， their correlation was analyzed statistically. Results: The expression of Bcl-2 and CD34 in the head，neck and body part of the pterygium had statistically significant differences( P ＜ 0. 01) . The expression of Bcl-2 and CD34 was higher in the head part than that in the neck and body part of the pterygium( P ＜ 0. 01) ; the expression of CD34 in the neck part was significantly higher than that in the body part( P ＜0. 01) . Conclusions: Bcl-2 and CD34 express high in the head，neck and body part of the pterygium，and express the highest in the head part. It suggests that the head part of the pterygium has less apoptosis than the other parts; but it is more proliferative and has the probability of excessive repair，which may the pathogenesis of the pterygium.
Objective: To investigate the expression of Bcl-2 and CD34 in the head，neck and body part of the primary pterygium， and to explore the pathogenesis of pterygium. Methods: The expressions of Bcl-2 and CD34 in 30 specimens from patients with primary pterygium and 5 specimens of normal bulbar conjunctiva were detected by immunohistochemical staining ( EliVision method) ， their correlation was analyzed statistically. Results: The expression of Bcl-2 and CD34 in the head，neck and body part of the pterygium had statistically significant differences( P ＜ 0. 01) . The expression of Bcl-2 and CD34 was higher in the head part than that in the neck and body part of the pterygium( P ＜ 0. 01) ; the expression of CD34 in the neck part was significantly higher than that in the body part( P ＜0. 01) . Conclusions: Bcl-2 and CD34 express high in the head，neck and body part of the pterygium，and express the highest in the head part. It suggests that the head part of the pterygium has less apoptosis than the other parts; but it is more proliferative and has the probability of excessive repair，which may the pathogenesis of the pterygium.
2013, 37(8): 995-997.
Abstract:
Objective: To investigate the effects and safety of pemetrexed combined with gefitinib in the treatment of patients with advanced non-small-cell lung cancer( NSCLC) with second-line treatment of epidermal growth factor receptor-tyrosine kinase inhibitor ( EGFr-TKI) . Methods: Twenty-eight patients with stage Ⅲb /Ⅳ NSCLC treated with second-line treatment of EGFr-TKI( gefitinib /erlotinib) were treated with 500 mg /m2 of pemetrexed and 250 mg of gefitinib per day( 21 days each cycle) until these cases were improved. Results: The median follow-up time was 12. 4 months， except 1 case without evaluation， complete response in 0. 0%，partial response in 37. 0%， stabilization of the disease in 44. 4% and progression of disease in 18. 5% were found. The disease control and total effective rates were 81. 5% and 37. 0%， respectively. The time of median without disease progression and survival time were 7. 0 months and 13. 6 months， respectively. No tumor progression and survival rate of 1 year were 33. 3% and 55. 6%， respectively. The occurrence rate of skin rash with more grade Ⅲ was 14. 3%. Conclusions: The effects and tolerance of pemetrexed combined with gefitinib in the treatment of patients with advanced NSCLC with second-line treatment of EGFr-TKI are good.
Objective: To investigate the effects and safety of pemetrexed combined with gefitinib in the treatment of patients with advanced non-small-cell lung cancer( NSCLC) with second-line treatment of epidermal growth factor receptor-tyrosine kinase inhibitor ( EGFr-TKI) . Methods: Twenty-eight patients with stage Ⅲb /Ⅳ NSCLC treated with second-line treatment of EGFr-TKI( gefitinib /erlotinib) were treated with 500 mg /m2 of pemetrexed and 250 mg of gefitinib per day( 21 days each cycle) until these cases were improved. Results: The median follow-up time was 12. 4 months， except 1 case without evaluation， complete response in 0. 0%，partial response in 37. 0%， stabilization of the disease in 44. 4% and progression of disease in 18. 5% were found. The disease control and total effective rates were 81. 5% and 37. 0%， respectively. The time of median without disease progression and survival time were 7. 0 months and 13. 6 months， respectively. No tumor progression and survival rate of 1 year were 33. 3% and 55. 6%， respectively. The occurrence rate of skin rash with more grade Ⅲ was 14. 3%. Conclusions: The effects and tolerance of pemetrexed combined with gefitinib in the treatment of patients with advanced NSCLC with second-line treatment of EGFr-TKI are good.
2013, 37(8): 998-999.
Abstract:
Objective: To observe the curative effects of the chemotherapy regimens of preoperative using cisplatin combined with different drugs in the treatment of cervical cancer. Methods: One hundred patients were randomly divided into treatment group Ⅰ and treatment group Ⅱ( 50 cases each group) . The treatment group Ⅰ were treated with cisplatin combined with capecitabine， the treatment group Ⅱ were treated with cisplatin combined with bleomycin. Two groups were performed radical surgical treatment. The treatment effectiveness and adverse reaction of two groups were analyzed after chemotherapy for 15 days. Results: The differences of the effective rate and side effects in the two groups were not statistical significance ( P ＞ 0. 05 ) . Conclusions: The chemotherapy regimen of preoperative using cisplatin combined with different drugs in the treatment of cervical cancer is feasible.
Objective: To observe the curative effects of the chemotherapy regimens of preoperative using cisplatin combined with different drugs in the treatment of cervical cancer. Methods: One hundred patients were randomly divided into treatment group Ⅰ and treatment group Ⅱ( 50 cases each group) . The treatment group Ⅰ were treated with cisplatin combined with capecitabine， the treatment group Ⅱ were treated with cisplatin combined with bleomycin. Two groups were performed radical surgical treatment. The treatment effectiveness and adverse reaction of two groups were analyzed after chemotherapy for 15 days. Results: The differences of the effective rate and side effects in the two groups were not statistical significance ( P ＞ 0. 05 ) . Conclusions: The chemotherapy regimen of preoperative using cisplatin combined with different drugs in the treatment of cervical cancer is feasible.
2013, 37(8): 1000-1002.
Abstract:
Objective: To investigate the factors related to the emergency hysterectomy，operation duration and the questions concerned. Methods: The clinical data of 18 cases having received hysterectomy in obstetric emergency from 2006 to 2012 were analyzedretrospectively. Results: Among the 18 cases，14 were due to placental factors( 77. 8%) ，3 uterine rupture( 6. 7%) and 1 amniotic fluid embolism( 5. 6%) . Eleven of the 18 patients were in a hemorrhagic shock state and had persisted refractory uterine bleeding. Conclusions: Multiple curettage，multiple pregnancy and cesarean section are the high risk factors for maternal hysterectomy;emergency hysterectomy might save the life of the patients. Forceful perinatal health care and low rate of cesarean section can effectivelyreduce the rate of emergency obstetric hysterectomy.
Objective: To investigate the factors related to the emergency hysterectomy，operation duration and the questions concerned. Methods: The clinical data of 18 cases having received hysterectomy in obstetric emergency from 2006 to 2012 were analyzedretrospectively. Results: Among the 18 cases，14 were due to placental factors( 77. 8%) ，3 uterine rupture( 6. 7%) and 1 amniotic fluid embolism( 5. 6%) . Eleven of the 18 patients were in a hemorrhagic shock state and had persisted refractory uterine bleeding. Conclusions: Multiple curettage，multiple pregnancy and cesarean section are the high risk factors for maternal hysterectomy;emergency hysterectomy might save the life of the patients. Forceful perinatal health care and low rate of cesarean section can effectivelyreduce the rate of emergency obstetric hysterectomy.
2013, 37(8): 1023-1024,1027.
Abstract:
Objective: To analyze and summarize the Results of ministry of health External Quality Assessment( EQA) for clinical chemistry laboratory of General Hospital of Wanbei Coal-electricity Group from 2008 to 2012， and to improve continuously the quality of clinical laboratory work and provide the better clinical service. Methods: According to the unified evaluation criteria of NCCL， the EQAResults of clinical chemistry laboratory were statistically analyzed using proficiency testing and variability index score( VIS) from 2008 to 2012. Results: From 2008 to 2012， the 5 years average qualified rate of proficiency testing was 98. 7%. VIS average score dropped from 53. 4 to 30. 2， the excellent，qualified and unqualified rates of VIS score were 85. 6%， 10. 1% and 4. 3%， respectively. Conclusions:The EQA analysis can improve the analysis capabilities of internal quality control and the examination quality， and provide the accurate basis for clinical diagnosis， therapy and prognosis.
Objective: To analyze and summarize the Results of ministry of health External Quality Assessment( EQA) for clinical chemistry laboratory of General Hospital of Wanbei Coal-electricity Group from 2008 to 2012， and to improve continuously the quality of clinical laboratory work and provide the better clinical service. Methods: According to the unified evaluation criteria of NCCL， the EQAResults of clinical chemistry laboratory were statistically analyzed using proficiency testing and variability index score( VIS) from 2008 to 2012. Results: From 2008 to 2012， the 5 years average qualified rate of proficiency testing was 98. 7%. VIS average score dropped from 53. 4 to 30. 2， the excellent，qualified and unqualified rates of VIS score were 85. 6%， 10. 1% and 4. 3%， respectively. Conclusions:The EQA analysis can improve the analysis capabilities of internal quality control and the examination quality， and provide the accurate basis for clinical diagnosis， therapy and prognosis.
2013, 37(8): 1025-1027.
Abstract:
Objective: To analyze the detection and distribution of vaginitis related pathogens of the outpatients in the gynecology department of our hospital. Methods: Four thousand four hundred and fifty-six vaginal discharge samples were detected of the fungus， bacteria， trichomonas and cleanness degree by vaginitis penta-pathogen detection kit. The detection rates in different age groups and the cleanness grades were compared. Results: The detection rate of various pathogens in patients with Ⅲ － Ⅳ grades of vaginal cleanness was higher than that in patients with Ⅰ － Ⅱ grades of vaginal cleanness ( P ＜ 0. 01) . The detection rate of fungus，bacteria and trichomonas in the age groups of 26 － 35 and 36 － 45 was higher than that in the age groups of 25， 46 － 55 and ＞ 55( P ＜ 0. 05) . Conclusions: The most common pathogens in the vaginitis of the outpatients in the department of gynecology were fungus，bacteria and trichmonas. Vaginal discharge with normal cleanness should also be detected of the pathogens.
Objective: To analyze the detection and distribution of vaginitis related pathogens of the outpatients in the gynecology department of our hospital. Methods: Four thousand four hundred and fifty-six vaginal discharge samples were detected of the fungus， bacteria， trichomonas and cleanness degree by vaginitis penta-pathogen detection kit. The detection rates in different age groups and the cleanness grades were compared. Results: The detection rate of various pathogens in patients with Ⅲ － Ⅳ grades of vaginal cleanness was higher than that in patients with Ⅰ － Ⅱ grades of vaginal cleanness ( P ＜ 0. 01) . The detection rate of fungus，bacteria and trichomonas in the age groups of 26 － 35 and 36 － 45 was higher than that in the age groups of 25， 46 － 55 and ＞ 55( P ＜ 0. 05) . Conclusions: The most common pathogens in the vaginitis of the outpatients in the department of gynecology were fungus，bacteria and trichmonas. Vaginal discharge with normal cleanness should also be detected of the pathogens.
2013, 37(8): 1031-1033.
Abstract:
Objective: To investigate the value of fetal nuchal translucency ( NT) thickness in screening early pregnancy fetal malformation. Methods: The fetal anatomical structure，NT thickness and nasal bone length in 2 306 pregnant women in 11 to 13 +6 weeks were detected by color doppler ultrasound. Results: Fetal malformation in 32 cases were found during pregnancy，which included 10 cases in 11 to 13 +6weeks of pregnancy and 22 cases in 13 +6 weeks after pregnancy. Fetal death in 13 cases，chromosomal abnormality in 3 cases and abnormal neonate in 4 cases were found. Five fetuses in 98 cases with thickening NT( more than 2. 5 mm) during 11 to 13 +6weeks of pregnancy were poor prognosis，which included chromosomal abnormality in 2 case，womb death in 2 cases and diaphragmatic hernia in 1 case， their fetal NT value was 4. 05 to 6. 20 mm. The normal fetal NT value was 2. 55 to 3. 60 mm. Conclusions: The ultrasound can diagnose some seriously fetuses malformation in 11 to 13 + 6 weeks of pregnancy. There is high possibility malformation in fetus with NT value more than 3. 6 mm.
Objective: To investigate the value of fetal nuchal translucency ( NT) thickness in screening early pregnancy fetal malformation. Methods: The fetal anatomical structure，NT thickness and nasal bone length in 2 306 pregnant women in 11 to 13 +6 weeks were detected by color doppler ultrasound. Results: Fetal malformation in 32 cases were found during pregnancy，which included 10 cases in 11 to 13 +6weeks of pregnancy and 22 cases in 13 +6 weeks after pregnancy. Fetal death in 13 cases，chromosomal abnormality in 3 cases and abnormal neonate in 4 cases were found. Five fetuses in 98 cases with thickening NT( more than 2. 5 mm) during 11 to 13 +6weeks of pregnancy were poor prognosis，which included chromosomal abnormality in 2 case，womb death in 2 cases and diaphragmatic hernia in 1 case， their fetal NT value was 4. 05 to 6. 20 mm. The normal fetal NT value was 2. 55 to 3. 60 mm. Conclusions: The ultrasound can diagnose some seriously fetuses malformation in 11 to 13 + 6 weeks of pregnancy. There is high possibility malformation in fetus with NT value more than 3. 6 mm.
2013, 37(8): 1034-1036.
Abstract:
Objective: To investigate the ultrasonographic characteristics of bicuspid aortic valve deformity. Methods: Seventy-one patients with bicuspid aortic valve deformity were divided into group Ⅰ( 9 cases， age less than 20 years) ， groupⅡ( 15 cases， age from 20 to 39 years) ，group Ⅲ( 31 cases，age from 40 to 59 years) and group Ⅳ( 16 cases，age more than 59 years) . The morphology，hemodynamics and cardiomorphology of all patients were observed by echocardiography. Results: The aortic valve calcification increased with the growth of age， group Ⅲ and Ⅳ were higher than group Ⅰ( P ＜ 0. 05 and P ＜ 0. 01) . The aortic stenosis and valvularinadequacy showed an upward trend with the age increasing，groupⅡ，Ⅲ and Ⅳ were higher than group Ⅰ( P ＜ 0. 05 to P ＜ 0. 01) . With the growth of age， the ascending aorta inner diameter and thickness of interventricular septum widened and thickened， groupⅡ，Ⅲ and Ⅳ were higher than group Ⅰ( P ＜ 0. 05 to P ＜ 0. 01) ， group Ⅲ and Ⅳ were higher than group Ⅱ( P ＜ 0. 05 to P ＜ 0. 01) . Anteroposterior diameter of the left ventricle increased， groupⅡ，Ⅲ and Ⅳ were higher than groupⅠ( P ＜ 0. 05 to P ＜ 0. 01) . Left ventricular posterior wall of four groups thickened， their differences of which were statistically significant( P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: Bicuspid aortic valve deformity is closely related to the patient's age. Echocardiography has important value in the diagnosis of bicuspid aortic valve deformity.
Objective: To investigate the ultrasonographic characteristics of bicuspid aortic valve deformity. Methods: Seventy-one patients with bicuspid aortic valve deformity were divided into group Ⅰ( 9 cases， age less than 20 years) ， groupⅡ( 15 cases， age from 20 to 39 years) ，group Ⅲ( 31 cases，age from 40 to 59 years) and group Ⅳ( 16 cases，age more than 59 years) . The morphology，hemodynamics and cardiomorphology of all patients were observed by echocardiography. Results: The aortic valve calcification increased with the growth of age， group Ⅲ and Ⅳ were higher than group Ⅰ( P ＜ 0. 05 and P ＜ 0. 01) . The aortic stenosis and valvularinadequacy showed an upward trend with the age increasing，groupⅡ，Ⅲ and Ⅳ were higher than group Ⅰ( P ＜ 0. 05 to P ＜ 0. 01) . With the growth of age， the ascending aorta inner diameter and thickness of interventricular septum widened and thickened， groupⅡ，Ⅲ and Ⅳ were higher than group Ⅰ( P ＜ 0. 05 to P ＜ 0. 01) ， group Ⅲ and Ⅳ were higher than group Ⅱ( P ＜ 0. 05 to P ＜ 0. 01) . Anteroposterior diameter of the left ventricle increased， groupⅡ，Ⅲ and Ⅳ were higher than groupⅠ( P ＜ 0. 05 to P ＜ 0. 01) . Left ventricular posterior wall of four groups thickened， their differences of which were statistically significant( P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: Bicuspid aortic valve deformity is closely related to the patient's age. Echocardiography has important value in the diagnosis of bicuspid aortic valve deformity.
2013, 37(8): 1037-1038,1041.
Abstract:
Objective: To observe the hemodynamic change of hepatic artery and portal vein in patients with hepatolenticular degeneration( HLD) complicated with hypersplenism before and after splenectomy. Methods: The hemodynamics of hepatic artery and portal vein in 46 patients with HLD complicated by hypersplenism were detected by color doppler ultrasound at preoperation and 7 and 14 days after splenectomy， all data were analyzed. Results: Compared with preoperation， the reducing diameter of main and right branch of portal vein and slowing of blood flow velocity were statistical significance at 7 days after splenectomy( P ＜ 0. 05 － P ＜ 0. 01) . The blood flow velocity of the right branch of portal vein at 14 days after splenectomy was higher than that in postoperative 7 days( P ＜0. 01) . There were not statistically significant in the diameter of main and right branch of portal vein and the peak velocity and resistance index of right hepatic artery between preoperation and postoperative 7 days and 14 days ( P ＞ 0. 05) . Conclusions: The hemodynamic change of hepatic artery and portal vein in patients with HLD complicated with hypersplenism after splenectomy is significant.
Objective: To observe the hemodynamic change of hepatic artery and portal vein in patients with hepatolenticular degeneration( HLD) complicated with hypersplenism before and after splenectomy. Methods: The hemodynamics of hepatic artery and portal vein in 46 patients with HLD complicated by hypersplenism were detected by color doppler ultrasound at preoperation and 7 and 14 days after splenectomy， all data were analyzed. Results: Compared with preoperation， the reducing diameter of main and right branch of portal vein and slowing of blood flow velocity were statistical significance at 7 days after splenectomy( P ＜ 0. 05 － P ＜ 0. 01) . The blood flow velocity of the right branch of portal vein at 14 days after splenectomy was higher than that in postoperative 7 days( P ＜0. 01) . There were not statistically significant in the diameter of main and right branch of portal vein and the peak velocity and resistance index of right hepatic artery between preoperation and postoperative 7 days and 14 days ( P ＞ 0. 05) . Conclusions: The hemodynamic change of hepatic artery and portal vein in patients with HLD complicated with hypersplenism after splenectomy is significant.
2013, 37(8): 1039-1041.
Abstract:
Objective: To investigate the clinical value of MＲI diffusion weighted imaging( DWI) combined with 3D-TOF magnetic resonance angiography( MＲA) in the diagnosis of hyperacute massive cerebral infarction. Methods: Eighty-three cases with acute cerebral infarction were scanned using conventional MＲI，DWI sequence and 3D-TOF MＲA， the lesions in different sequence MＲI were compared and analyzed. Results: The T1WI and T2WI of 51 cases with onset time less than 2 h and 13 cases with onset time between 2 － 6 h were normal. The T2WI of 19 cases with onset time between 2 － 6 h were slightly high signal. All DWI were high signal. The artery stenosis and occlusion in 78 cases were found by 3D-TOF MＲA，which included 7 cases of internal carotid artery occlusion， 19 cases of middle cerebral artery occlusion and 10 cases of posterior cerebral artery occlusion. Conclusions: DWI can diagnose acute cerebral infarction and identify its location. DWI combined with 3D-TOF MＲA technology can quickly and accurately show the intracranial artery stenosis or occlusion and corresponding cerebral infarction.
Objective: To investigate the clinical value of MＲI diffusion weighted imaging( DWI) combined with 3D-TOF magnetic resonance angiography( MＲA) in the diagnosis of hyperacute massive cerebral infarction. Methods: Eighty-three cases with acute cerebral infarction were scanned using conventional MＲI，DWI sequence and 3D-TOF MＲA， the lesions in different sequence MＲI were compared and analyzed. Results: The T1WI and T2WI of 51 cases with onset time less than 2 h and 13 cases with onset time between 2 － 6 h were normal. The T2WI of 19 cases with onset time between 2 － 6 h were slightly high signal. All DWI were high signal. The artery stenosis and occlusion in 78 cases were found by 3D-TOF MＲA，which included 7 cases of internal carotid artery occlusion， 19 cases of middle cerebral artery occlusion and 10 cases of posterior cerebral artery occlusion. Conclusions: DWI can diagnose acute cerebral infarction and identify its location. DWI combined with 3D-TOF MＲA technology can quickly and accurately show the intracranial artery stenosis or occlusion and corresponding cerebral infarction.
2013, 37(8): 1042-1044.
Abstract:
Objective: To explore the application value of 128-slice spiral CT angiography in the diagnosis of acute chest pain. Methods: Acute chest pain in 36 patients were highly suspected to be caused by acute myocardial infarction，pulmonary embolism and aortic dissection aneurysm. All cases were detected by 128-slice spiral CT angiography. The vessels were remodeled by volume rendering，multiplanar reconstruction and high density projection technique， the anatomical lesions in pulmonary artery，pulmonary vein， coronary and aorta were observed through different angles. Results: The 3D images of pulmonary artery，pulmonary vein， left and right coronary artery and aorta of all cases could be clearly displayed. Coronary artery stenosis and occlusion in 13 cases，aortic dissecting aneurysm in 5 cases，pulmonary artery embolism in 5 cases， other diseases in 9 cases and normal in 4 cases were found. Conclusions:128-slice spiral CT scaning can determine the diagnosis of large vessel disease in pulmonary artery，pulmonary vein， coronary artery and aorta，which has the important significance in the clinical diagnosis and treatment of chest pain triad.
Objective: To explore the application value of 128-slice spiral CT angiography in the diagnosis of acute chest pain. Methods: Acute chest pain in 36 patients were highly suspected to be caused by acute myocardial infarction，pulmonary embolism and aortic dissection aneurysm. All cases were detected by 128-slice spiral CT angiography. The vessels were remodeled by volume rendering，multiplanar reconstruction and high density projection technique， the anatomical lesions in pulmonary artery，pulmonary vein， coronary and aorta were observed through different angles. Results: The 3D images of pulmonary artery，pulmonary vein， left and right coronary artery and aorta of all cases could be clearly displayed. Coronary artery stenosis and occlusion in 13 cases，aortic dissecting aneurysm in 5 cases，pulmonary artery embolism in 5 cases， other diseases in 9 cases and normal in 4 cases were found. Conclusions:128-slice spiral CT scaning can determine the diagnosis of large vessel disease in pulmonary artery，pulmonary vein， coronary artery and aorta，which has the important significance in the clinical diagnosis and treatment of chest pain triad.
2013, 37(8): 1045-1046,1049.
Abstract:
Objective: To investigate the value of the high-frequency superficial small organ probe combined with cavity probe in the diagnosis of perianal abscess. Methods: The perianal abscess was detected using 5 to 8 MHz of high-frequency probe and 6 MHz of cavity probe( observation group) ， the Results of which were compared with conventional specialist examination( control group) . Results:The coincidence rate of inlet location and relation with sphincter， and the diagnosic accuracy rate of observation group were better than those in control group( P ＜ 0. 05 － P ＜ 0. 01) . Conclusions: The high-frequency superficial small organ probe combined with cavity probe in the detection of perianal abscess has unique advantage，which can provide Objective basis in the diagnosis of perianal abscess.
Objective: To investigate the value of the high-frequency superficial small organ probe combined with cavity probe in the diagnosis of perianal abscess. Methods: The perianal abscess was detected using 5 to 8 MHz of high-frequency probe and 6 MHz of cavity probe( observation group) ， the Results of which were compared with conventional specialist examination( control group) . Results:The coincidence rate of inlet location and relation with sphincter， and the diagnosic accuracy rate of observation group were better than those in control group( P ＜ 0. 05 － P ＜ 0. 01) . Conclusions: The high-frequency superficial small organ probe combined with cavity probe in the detection of perianal abscess has unique advantage，which can provide Objective basis in the diagnosis of perianal abscess.
2013, 37(8): 1047-1049.
Abstract:
Objective: To analyze the clinical symptoms and CT findings of fungous sinusitis， and to assess the value of CT in diagnosis of the disease. Methods: The clinical symptoms and CT features of 25 cases of fungous sinusitis which had been proved by pathology after operation were analyzed retrospectively. Results: The main clinical features of fungous sinusitis were nasal obstruction，headache，purulent nasal discharge，suction of blood mucus and olfactory abnormalities; CT scans showed inhomogeneous soft tissue density involving the unilateral or bilateral， single or multiple paranasal sinus cavities，and sinus cavity narrowing. Plaque， spotted and strip calcification were observed in the sinus cavity of 19 cases，and 13 cases were accompanied by enlarged maxillary sinus ostium and 4 cases bone absorption wall thinning in the maxillary sinus. Conclusions: The diagnosis of fungous sinusitis relies mainly on the clinical symptoms and CT examination. The lesion density， scope and paranasal sinus anatomic structure can be displayed clearly in CT scan. Abnormal calcification in the lesion is a reliable sign for the diagnosis of fungous sinusitis， and CT scan is the first and the most effective diagnostic method.
Objective: To analyze the clinical symptoms and CT findings of fungous sinusitis， and to assess the value of CT in diagnosis of the disease. Methods: The clinical symptoms and CT features of 25 cases of fungous sinusitis which had been proved by pathology after operation were analyzed retrospectively. Results: The main clinical features of fungous sinusitis were nasal obstruction，headache，purulent nasal discharge，suction of blood mucus and olfactory abnormalities; CT scans showed inhomogeneous soft tissue density involving the unilateral or bilateral， single or multiple paranasal sinus cavities，and sinus cavity narrowing. Plaque， spotted and strip calcification were observed in the sinus cavity of 19 cases，and 13 cases were accompanied by enlarged maxillary sinus ostium and 4 cases bone absorption wall thinning in the maxillary sinus. Conclusions: The diagnosis of fungous sinusitis relies mainly on the clinical symptoms and CT examination. The lesion density， scope and paranasal sinus anatomic structure can be displayed clearly in CT scan. Abnormal calcification in the lesion is a reliable sign for the diagnosis of fungous sinusitis， and CT scan is the first and the most effective diagnostic method.
2013, 37(8): 1050-1051,1055.
Abstract:
Objective: To investigate the effects of a new simple device for blood collection by tail snipping in mice for providing a safey， convenient and economical method. Methods: The device was made by some hard paper，gauze，and two centrifuge tubes( 50 ml) ， and which were fixed on a plastic plate. The blood of 50 Kunming mice aged 6 to 8 weeks were collected by tail snipping for repeat 4 times. Results: The device was cheap， and easy to be made and handled，which could collect blood for two mice at the sametime. No mouse was disabled or dead during the experimental course. Conclusions: The device is a safe and ideal equipment for bloodcollection by tail snipping in mice.
Objective: To investigate the effects of a new simple device for blood collection by tail snipping in mice for providing a safey， convenient and economical method. Methods: The device was made by some hard paper，gauze，and two centrifuge tubes( 50 ml) ， and which were fixed on a plastic plate. The blood of 50 Kunming mice aged 6 to 8 weeks were collected by tail snipping for repeat 4 times. Results: The device was cheap， and easy to be made and handled，which could collect blood for two mice at the sametime. No mouse was disabled or dead during the experimental course. Conclusions: The device is a safe and ideal equipment for bloodcollection by tail snipping in mice.
2013, 37(8): 1052-1055.
Abstract:
Objective: To compare and define the suitable range of the different Methods for isolation of murine hepatic lymphocyte，natural killer T cells( NKT cells) and natural killer cells( NK cells) . Methods: According to factorial design， four liver pretreatmentMethods including unbleeding method( A method) ， the eyeballs bleeding method( B method) ，body perfusion method( C method) ， the eyeball with body perfusion method( B method + C method) and two percoll isolation Methods including single concentration method( Ⅰ method) and continuous density gradient method( Ⅱ method) had been combined into 8 kinds of combination conditions， four bleeding Methods were used to prepare cell suspension respectively，and the single concentration method ( 33% Percoll) and the continuous density gradient method( 33% Percoll + 70% Percoll) were used to separation of mouse hepatic lymphocytes respectively，the hepatic innate immune cells were analyzed with two or three-colors immunofluorescent-labeling. Results: With the analysis of variance of factorial design， the highest proportion of lymphocytes was obtained by B method + C method， the highest absolute lymphocyte count was obtained by A method， the highest NKT and NK cell percentage and absolute number was obtained through B method; the ratios and the number of lymphocyte，T cell，NKT cell，NK cell obtained from B method were higher than those of A method. The ratio of NKT cell and NK cell absolute count， liver pretreatment method and percoll concentration had interactions each other( P ＜0. 01 and P＜0. 05)， the others were no interaction( P ＞0. 05) . Conclusions: High proportion of natural immune cells can be obtained by bleeding with perfusion combined with the continuous density gradient method( 33% + 70%)， it is helpful for research on NKT cells and NK cells.
Objective: To compare and define the suitable range of the different Methods for isolation of murine hepatic lymphocyte，natural killer T cells( NKT cells) and natural killer cells( NK cells) . Methods: According to factorial design， four liver pretreatmentMethods including unbleeding method( A method) ， the eyeballs bleeding method( B method) ，body perfusion method( C method) ， the eyeball with body perfusion method( B method + C method) and two percoll isolation Methods including single concentration method( Ⅰ method) and continuous density gradient method( Ⅱ method) had been combined into 8 kinds of combination conditions， four bleeding Methods were used to prepare cell suspension respectively，and the single concentration method ( 33% Percoll) and the continuous density gradient method( 33% Percoll + 70% Percoll) were used to separation of mouse hepatic lymphocytes respectively，the hepatic innate immune cells were analyzed with two or three-colors immunofluorescent-labeling. Results: With the analysis of variance of factorial design， the highest proportion of lymphocytes was obtained by B method + C method， the highest absolute lymphocyte count was obtained by A method， the highest NKT and NK cell percentage and absolute number was obtained through B method; the ratios and the number of lymphocyte，T cell，NKT cell，NK cell obtained from B method were higher than those of A method. The ratio of NKT cell and NK cell absolute count， liver pretreatment method and percoll concentration had interactions each other( P ＜0. 01 and P＜0. 05)， the others were no interaction( P ＞0. 05) . Conclusions: High proportion of natural immune cells can be obtained by bleeding with perfusion combined with the continuous density gradient method( 33% + 70%)， it is helpful for research on NKT cells and NK cells.
2013, 37(8): 1056-1059.
Abstract:
Objective: To investigate the virus infected patients with acute respiratory infection in Fengtai district of Beijing. Methods: The adenoviruses，human coronary virus and metapneumovirus were detected using ＲT-PCＲ. The respiratory syncytial virus， parainfluenza virus( PIV) ， rhinovirus， enterovirus and human bocavirus were detected using nested-PCＲ. The influenza A virus( IVA，including A1，A3 and swine flu type) and influenza B virus were detected using Ｒealtime-PCＲ. Results: Among 227 specimens，111specimens with postive virus were detected， the postive detection rate was 48. 90%. The influenza virus detection rate was 61. 26% inpostive virus specimens，which was the highest， IVB was given priority to， followed by influenza A3. The PIV detection rate was the second，PIV1 was given priority to， followed by PIV4， all virus infection were clustering. The positive rate in winter was higher than other seasons， the positive rate in patients with upper respiratory infection was higher than other patients. Conclusions: IVB is the main pathogeny of respiratory infection in 2011 and 2012 winter. Winter is high season of respiratory virus， the main route of virus infection is upper respiratory infection.
Objective: To investigate the virus infected patients with acute respiratory infection in Fengtai district of Beijing. Methods: The adenoviruses，human coronary virus and metapneumovirus were detected using ＲT-PCＲ. The respiratory syncytial virus， parainfluenza virus( PIV) ， rhinovirus， enterovirus and human bocavirus were detected using nested-PCＲ. The influenza A virus( IVA，including A1，A3 and swine flu type) and influenza B virus were detected using Ｒealtime-PCＲ. Results: Among 227 specimens，111specimens with postive virus were detected， the postive detection rate was 48. 90%. The influenza virus detection rate was 61. 26% inpostive virus specimens，which was the highest， IVB was given priority to， followed by influenza A3. The PIV detection rate was the second，PIV1 was given priority to， followed by PIV4， all virus infection were clustering. The positive rate in winter was higher than other seasons， the positive rate in patients with upper respiratory infection was higher than other patients. Conclusions: IVB is the main pathogeny of respiratory infection in 2011 and 2012 winter. Winter is high season of respiratory virus， the main route of virus infection is upper respiratory infection.
2013, 37(8): 1063-1065.
Abstract:
Objective: To discuss the effect of PDCA cycle management in the cleaning and disinfection quality in the department of stomatology. Methods: PDCA cycle management( planning， implement， checking and manipulation) was adopted to analyze the problems existing in the cleaning and disinfection room of the stomatology department，and the effects were evaluated. Results: After the application of PDCA cycle management， the cleaning and disinfection quality in the stomatology department was significantly improved;the conformity rate of the apparatus's cleaning，packing and sterilization reached 100. 00%，which was significantly higher than before( P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: PDCA cycle management is effective in improving the cleaning and disinfection quality in the sterilizing room.
Objective: To discuss the effect of PDCA cycle management in the cleaning and disinfection quality in the department of stomatology. Methods: PDCA cycle management( planning， implement， checking and manipulation) was adopted to analyze the problems existing in the cleaning and disinfection room of the stomatology department，and the effects were evaluated. Results: After the application of PDCA cycle management， the cleaning and disinfection quality in the stomatology department was significantly improved;the conformity rate of the apparatus's cleaning，packing and sterilization reached 100. 00%，which was significantly higher than before( P ＜ 0. 05 to P ＜ 0. 01) . Conclusions: PDCA cycle management is effective in improving the cleaning and disinfection quality in the sterilizing room.
2013, 37(8): 1068-1070.
Abstract:
Objective: To carry out targeted training by surveying the apprentice nurses' mastery of hospital infection knowledge. Methods: A total of 180 apprentice nurses were investigated about the hospital infection knowledge by a questionnaire before and after the training. Results: The score increased significantly after training compared to that before， the difference was statistically significant ( P ＜ 0. 01) . Conclusions: The apprentice nurses' knowledge about hospital infection is quite deficient before training. Pro-job training can significantly improve their mastery of hospital infection knowledge，which is essential in prevention and control of hospital infections.
Objective: To carry out targeted training by surveying the apprentice nurses' mastery of hospital infection knowledge. Methods: A total of 180 apprentice nurses were investigated about the hospital infection knowledge by a questionnaire before and after the training. Results: The score increased significantly after training compared to that before， the difference was statistically significant ( P ＜ 0. 01) . Conclusions: The apprentice nurses' knowledge about hospital infection is quite deficient before training. Pro-job training can significantly improve their mastery of hospital infection knowledge，which is essential in prevention and control of hospital infections.
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