Author Login
Experts Review
Academic Editor
Editor-in-chief
2016 Vol. 41, No. 3
Display Method:
2016, 41(3): 281-283.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.001
Abstract:
Objective:To explore the effects of melatonin combined with cisplatin on the proliferation and apoptosis of Eca109 cell and study the molecular mechanism.Methods:Eca109 cells were con-cultured with different concentrations of melatonin with or without cisplatin for 24 h.The inhibitory rate of Eca109 cells were examined using MTT assay, and the cell apoptosis was analyzed using flow cytometry with propidium iodide staining.Western blot for apoptosis-related protein Bcl-2 and Bax were used to analyze possible mechanisms of the synergistic anti-proliferation effect of melatonin in combination with cisplatin.Results:Different concentrations of melatonin combined with cisplatin showed an obviously enhanced inhibitory effect on colony formation of Eca109 cells.When melatonin combined with cisplatin, the cell apoptosis rate increased to 29%, significantly higher than that in cells with cisplatin treatment alone(9.7%)(P<0.01).The expression of Bcl-2 was high but Bax was low in Eca109 cells, after stimulating with melatonin and cisplatin, the expression of Bcl-2 decreased but expression of Bax increased.Conclusions:Melatonin can enhance the anti-proliferative effect of cisplatin on Eca109 cell and can enhance cisplatin-induced apoptosis.The possible mechanism may related to the downregulation of Bcl-2 and upregulation of Bax protein expression.
Objective:To explore the effects of melatonin combined with cisplatin on the proliferation and apoptosis of Eca109 cell and study the molecular mechanism.Methods:Eca109 cells were con-cultured with different concentrations of melatonin with or without cisplatin for 24 h.The inhibitory rate of Eca109 cells were examined using MTT assay, and the cell apoptosis was analyzed using flow cytometry with propidium iodide staining.Western blot for apoptosis-related protein Bcl-2 and Bax were used to analyze possible mechanisms of the synergistic anti-proliferation effect of melatonin in combination with cisplatin.Results:Different concentrations of melatonin combined with cisplatin showed an obviously enhanced inhibitory effect on colony formation of Eca109 cells.When melatonin combined with cisplatin, the cell apoptosis rate increased to 29%, significantly higher than that in cells with cisplatin treatment alone(9.7%)(P<0.01).The expression of Bcl-2 was high but Bax was low in Eca109 cells, after stimulating with melatonin and cisplatin, the expression of Bcl-2 decreased but expression of Bax increased.Conclusions:Melatonin can enhance the anti-proliferative effect of cisplatin on Eca109 cell and can enhance cisplatin-induced apoptosis.The possible mechanism may related to the downregulation of Bcl-2 and upregulation of Bax protein expression.
2016, 41(3): 284-287.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.002
Abstract:
Objective:To explore the effects of tetramethylpyrazine(TMP) on the levels of RhoA, Rho associated coiled coil forming protein kinase 2(ROCK2), myosin light chain(MLC) phosphorylation and ezrin-radxin-moesin(ERM) phosphorylation in the injury of human umbilical vein endothelial cells(HUVECs) induced by lipopolysaccharide(LPS).Methods:The HUVECs were cultured in vitro, and divided into the control group, LPS group and TMP groups(0.5, 1.0, 1.5 mg/mL).The mRNA levels of RhoA, ROCK2 and p-EZR in endothelial cells were detected by real-time fluorescence quantitative PCR, and the protein levels of RhoA, ROCK2, p-MLC and p-ERM were examined using western blotting.Results:Compared with the control group, the protein levels of RhoA, ROCK2, p-MLC and p-ERM, and the mRNA levels of RhoA, ROCK2 and p-Ezr in LPS group significantly increased(P<0.01).The differences of the protein and mRNA levels of RhoA between LPS group and 3 TMP groups were not statistically significant(P>0.05).Compared with the LPS group, the protein levels of ROCK2, p-MLC and p-ERM, and the mRNA levels of ROCK2 and p-Ezr decreased significantly in 3 TMP groups(P<0.01).The differences of the expression of ROCK2, p-MLC and p-ERM within 3 TMP groups were statistically significant(P<0.05 to P<0.01).Conclusions:TMP may protect the HUVECs against LPS-induced injury by inhibiting the expressions of ROCK2, p-MLC and p-ERM in Rho/ROCK signaling pathways, and reducing the injury of LPS on endothelial cell skeleton.
Objective:To explore the effects of tetramethylpyrazine(TMP) on the levels of RhoA, Rho associated coiled coil forming protein kinase 2(ROCK2), myosin light chain(MLC) phosphorylation and ezrin-radxin-moesin(ERM) phosphorylation in the injury of human umbilical vein endothelial cells(HUVECs) induced by lipopolysaccharide(LPS).Methods:The HUVECs were cultured in vitro, and divided into the control group, LPS group and TMP groups(0.5, 1.0, 1.5 mg/mL).The mRNA levels of RhoA, ROCK2 and p-EZR in endothelial cells were detected by real-time fluorescence quantitative PCR, and the protein levels of RhoA, ROCK2, p-MLC and p-ERM were examined using western blotting.Results:Compared with the control group, the protein levels of RhoA, ROCK2, p-MLC and p-ERM, and the mRNA levels of RhoA, ROCK2 and p-Ezr in LPS group significantly increased(P<0.01).The differences of the protein and mRNA levels of RhoA between LPS group and 3 TMP groups were not statistically significant(P>0.05).Compared with the LPS group, the protein levels of ROCK2, p-MLC and p-ERM, and the mRNA levels of ROCK2 and p-Ezr decreased significantly in 3 TMP groups(P<0.01).The differences of the expression of ROCK2, p-MLC and p-ERM within 3 TMP groups were statistically significant(P<0.05 to P<0.01).Conclusions:TMP may protect the HUVECs against LPS-induced injury by inhibiting the expressions of ROCK2, p-MLC and p-ERM in Rho/ROCK signaling pathways, and reducing the injury of LPS on endothelial cell skeleton.
2016, 41(3): 288-291.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.003
Abstract:
Objective:To detect the changes of the different stimulants on the peripheral blood T cell subsets of healthy people and tuberculosis(TB) patients producing interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α).Methods:The proportion of T cell subsets producing INF-γ and TNF-α in 12 healthy people peripheral blood mononuclear cells and 12 TB patients peripheral blood stimulated with PMA combined with ionomycin, Mtb-Hag, PHA and blocked with monensin were detected using the flow cytometry.Results:The proportion of INF-γ produced by αβ T cells of peripheral blood stimulated with PMA in healthy people was significantly higher than that in TB patients(P<0.05).The proportions of INF-γ produced by γδ T cells of peripheral blood stimulated with PMA, Mtb-Hag and PHA in healthy people were higher than that in TB patients(P<0.05 to P<0.01).The proportions of TNF-α produced by αβ T cells of peripheral blood stimulated with PMA PHA in healthy people were higher than those in TB patients(P<0.05).The proportions of INF-α produced by γδ T cells of peripheral blood stimulated with PMA and PHA in healthy people were higher than that in TB patients(P<0.05).Conclusions:After stimulated with different stimulants, the proportion of INF-γ produced by γδ T cells in healthy people is higher than that in TB patients.After simulated with PMA and PHA, the proportion of TNF-α produced by αβ T cells and γδ T cells in healthy people is higher than that in TB patients.
Objective:To detect the changes of the different stimulants on the peripheral blood T cell subsets of healthy people and tuberculosis(TB) patients producing interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α).Methods:The proportion of T cell subsets producing INF-γ and TNF-α in 12 healthy people peripheral blood mononuclear cells and 12 TB patients peripheral blood stimulated with PMA combined with ionomycin, Mtb-Hag, PHA and blocked with monensin were detected using the flow cytometry.Results:The proportion of INF-γ produced by αβ T cells of peripheral blood stimulated with PMA in healthy people was significantly higher than that in TB patients(P<0.05).The proportions of INF-γ produced by γδ T cells of peripheral blood stimulated with PMA, Mtb-Hag and PHA in healthy people were higher than that in TB patients(P<0.05 to P<0.01).The proportions of TNF-α produced by αβ T cells of peripheral blood stimulated with PMA PHA in healthy people were higher than those in TB patients(P<0.05).The proportions of INF-α produced by γδ T cells of peripheral blood stimulated with PMA and PHA in healthy people were higher than that in TB patients(P<0.05).Conclusions:After stimulated with different stimulants, the proportion of INF-γ produced by γδ T cells in healthy people is higher than that in TB patients.After simulated with PMA and PHA, the proportion of TNF-α produced by αβ T cells and γδ T cells in healthy people is higher than that in TB patients.
2016, 41(3): 292-295.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.004
Abstract:
Objective:To obtain the stereotactic anatomy data set of the marginal ramus in cingulate sulcus.Methods:The most medial end of marginal ramus of the cingulate sulcus was set as a starting point in Descartes coordinate system, the each point at interval of 3 mm was identified, the X and Y coordinate values of which was recorded.The multiplication of the number of plane distancing from zero plane and plane distance was set as the Z value.Results:The three-dimensional anatomy data set of the marginal ramus in cingulate sulcus was constructed in the three-dimensional coordinates.Conclusions:The positional relationship between marginal ramus of the cingulate sulcus and Descartes three-dimensional coordinate is stable.
Objective:To obtain the stereotactic anatomy data set of the marginal ramus in cingulate sulcus.Methods:The most medial end of marginal ramus of the cingulate sulcus was set as a starting point in Descartes coordinate system, the each point at interval of 3 mm was identified, the X and Y coordinate values of which was recorded.The multiplication of the number of plane distancing from zero plane and plane distance was set as the Z value.Results:The three-dimensional anatomy data set of the marginal ramus in cingulate sulcus was constructed in the three-dimensional coordinates.Conclusions:The positional relationship between marginal ramus of the cingulate sulcus and Descartes three-dimensional coordinate is stable.
2016, 41(3): 299-301.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.006
Abstract:
Objective:To compare the clinical effects between intervention guided by ultrasound and surgery in the treatment of lymphatic malformations(LMs).Methods:Among the thirty-five LMs patients diagnosed by ultrasound examination, 17 cases were treated with bleomycin intervention guided by ultrasound(intervention group), and 18 cases were treated with surgery(operation group).The clinical effect, recurrence rate and postoperative complication between groups were followed up.Results:The clinical effect in intervention group was better than those in operation group(P<0.05).The recurrence rates in intervention group and operation group were 11.76% and 33.33%, respectively.The difference of which was not statistically significant(P=0.229).No surgical scar, dysfunction and pulmonary fibrosis, and local infection in 1 case and fever in 8 cases in intervention group were found.No wound infection, important nerve damage and dysfunction, and wound exudate in 4 cases and operation scar in all cases in operation group were identified.Conclusions:The treatment of LMs with intervention guided by ultrasound is accurate, good efficacy, fewer complications and minimally invasive.
Objective:To compare the clinical effects between intervention guided by ultrasound and surgery in the treatment of lymphatic malformations(LMs).Methods:Among the thirty-five LMs patients diagnosed by ultrasound examination, 17 cases were treated with bleomycin intervention guided by ultrasound(intervention group), and 18 cases were treated with surgery(operation group).The clinical effect, recurrence rate and postoperative complication between groups were followed up.Results:The clinical effect in intervention group was better than those in operation group(P<0.05).The recurrence rates in intervention group and operation group were 11.76% and 33.33%, respectively.The difference of which was not statistically significant(P=0.229).No surgical scar, dysfunction and pulmonary fibrosis, and local infection in 1 case and fever in 8 cases in intervention group were found.No wound infection, important nerve damage and dysfunction, and wound exudate in 4 cases and operation scar in all cases in operation group were identified.Conclusions:The treatment of LMs with intervention guided by ultrasound is accurate, good efficacy, fewer complications and minimally invasive.
2016, 41(3): 302-304,307.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.007
Abstract:
Objective:To explore the causes of nonspecific chronic cough in children 5 or under 5 years old for improving its diagnosis and treatment.Methods:The clinical data of 85 children 5 or under 5 years old with nonspecific chronic cough were retrospectively analyzed.Results:The etiologic distribution of the 85 children with nonspecific chronic cough included 27 cases of cough variant asthma(31.8%), 32 cases of upper airway cough syndrome(37.6%), 16 cases of post-infection cough(18.8%), 3 cases of gastroesophageal reflux cough(3.5%), 2 cases of atopic cough(2.4%) and 5 cases of unknown etiology(5.9%).Conclusions:The main causes of nonspecific chronic cough in children 5 or under 5 years old are the upper airway cough syndrome, cough variant asthma, post-infection cough and other causes in turn.
Objective:To explore the causes of nonspecific chronic cough in children 5 or under 5 years old for improving its diagnosis and treatment.Methods:The clinical data of 85 children 5 or under 5 years old with nonspecific chronic cough were retrospectively analyzed.Results:The etiologic distribution of the 85 children with nonspecific chronic cough included 27 cases of cough variant asthma(31.8%), 32 cases of upper airway cough syndrome(37.6%), 16 cases of post-infection cough(18.8%), 3 cases of gastroesophageal reflux cough(3.5%), 2 cases of atopic cough(2.4%) and 5 cases of unknown etiology(5.9%).Conclusions:The main causes of nonspecific chronic cough in children 5 or under 5 years old are the upper airway cough syndrome, cough variant asthma, post-infection cough and other causes in turn.
2016, 41(3): 305-307.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.008
Abstract:
Objective:To compare the effects of partial cystectomy and radical cystectomy in the treatment of the T2M0N0 stage bladder cancer.Methods:Sixty patients with T2M0N0 stage bladder cancer were divided into the partial cystectomy group(A group) and bladder radical cystectomy(B group)(30 cases each group).The hospitalization time, operation time, intraoperative bleeding volume, 5-year survival rate and postoperative life quality between two groups were compared.Results:The hospitalized time, operation time and intraoperative bleeding volume in A group were significantly less than those in B group(P<0.01).Evaluation of life quality after operation showed that the overall health in A group was better than that in B group(P<0.01).The difference of the 5-year survival rate between two groups was not statistically significant(P>0.05).Conclusions:The T2M0N0 stage bladder cancer should be treated with the partial cystectomy, the operation time, hospitalized time, intraoperative bleeding volume, postoperative quality of life of which are better than those in radical cystectomy, and the 5-year survival rate between partial cystectomy and radical cystectomy is similar.
Objective:To compare the effects of partial cystectomy and radical cystectomy in the treatment of the T2M0N0 stage bladder cancer.Methods:Sixty patients with T2M0N0 stage bladder cancer were divided into the partial cystectomy group(A group) and bladder radical cystectomy(B group)(30 cases each group).The hospitalization time, operation time, intraoperative bleeding volume, 5-year survival rate and postoperative life quality between two groups were compared.Results:The hospitalized time, operation time and intraoperative bleeding volume in A group were significantly less than those in B group(P<0.01).Evaluation of life quality after operation showed that the overall health in A group was better than that in B group(P<0.01).The difference of the 5-year survival rate between two groups was not statistically significant(P>0.05).Conclusions:The T2M0N0 stage bladder cancer should be treated with the partial cystectomy, the operation time, hospitalized time, intraoperative bleeding volume, postoperative quality of life of which are better than those in radical cystectomy, and the 5-year survival rate between partial cystectomy and radical cystectomy is similar.
2016, 41(3): 308-311.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.009
Abstract:
Objective:To evaluate the clinical effects of the external fixator combined with Kirschner wire in the treatment of complex Barton fracture.Methods:Twenty-one patients with complex Barton fractures were treated with the close reduction or limited-open reduction, and external fixator combined with Kirschner wire.All patients were followed up for 6 to 36 months.Results:The fractures healed after 3 to 8 weeks of operation, and osseous healing was found during 3 to 4 months.According to Gartland-Werley score, the excellent in 15 cases, good in 4 cases and general in 2 cases were identified, the excellent rate was 90.47%.The average DASH questionnaire score was 15 points.Conclusions:The external fixator combined with Kirschner wire can provide a new approach in treating complex Barton fractures.
Objective:To evaluate the clinical effects of the external fixator combined with Kirschner wire in the treatment of complex Barton fracture.Methods:Twenty-one patients with complex Barton fractures were treated with the close reduction or limited-open reduction, and external fixator combined with Kirschner wire.All patients were followed up for 6 to 36 months.Results:The fractures healed after 3 to 8 weeks of operation, and osseous healing was found during 3 to 4 months.According to Gartland-Werley score, the excellent in 15 cases, good in 4 cases and general in 2 cases were identified, the excellent rate was 90.47%.The average DASH questionnaire score was 15 points.Conclusions:The external fixator combined with Kirschner wire can provide a new approach in treating complex Barton fractures.
2016, 41(3): 311-313.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.010
Abstract:
Objective:To explore the protective effects of the nano carbon technology on the parathyroid during the operation of thyroid cancer.Methods:Fifty patients with thyroid carcinoma were randomly divided into the control group and nano carbon group.Parathyroid mistakenly cut in the lymph adipose tissue, postoperative blood calcium and parathyroid hormone levelss, and the incidence of clinical symptoms of postoperative parathyroid injury between two groups were compared.Results:The parathyroid gland in black dye organization in nano carbon group was not found.The incidence of postoperative clinical symptoms of parathyroid damage in nano carbon group was less than that in control group(P<0.05 to P<0.01).The levels of blood calcium and parathyroid hormone in nano carbon group were higher than those in control group at d 1, d 3 and 3 months of operation(P<0.05 to P<0.01).Conclusions:The lymphatic drainage way of thyroid and parathyroid is different.The nano carbon tracer in thyroid lymphatic system can well identify parathyroid gland, reduce the damage probability of parathyroid gland during the thyroid cancer lymph node cleaning.
Objective:To explore the protective effects of the nano carbon technology on the parathyroid during the operation of thyroid cancer.Methods:Fifty patients with thyroid carcinoma were randomly divided into the control group and nano carbon group.Parathyroid mistakenly cut in the lymph adipose tissue, postoperative blood calcium and parathyroid hormone levelss, and the incidence of clinical symptoms of postoperative parathyroid injury between two groups were compared.Results:The parathyroid gland in black dye organization in nano carbon group was not found.The incidence of postoperative clinical symptoms of parathyroid damage in nano carbon group was less than that in control group(P<0.05 to P<0.01).The levels of blood calcium and parathyroid hormone in nano carbon group were higher than those in control group at d 1, d 3 and 3 months of operation(P<0.05 to P<0.01).Conclusions:The lymphatic drainage way of thyroid and parathyroid is different.The nano carbon tracer in thyroid lymphatic system can well identify parathyroid gland, reduce the damage probability of parathyroid gland during the thyroid cancer lymph node cleaning.
2016, 41(3): 314-316,319.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.011
Abstract:
Objective:To investigate the expressions of aquaporin1(AQP1) and vascular endothelial growth factor(VEGF) in cervical squamous cell carcinoma tissue and their correlations.Methods:The expressions of AQP1 and VEGF in 41 samples of cervical squamous cell carcinoma tissues, 55 samples of cervical intraepithelial neoplasisa(CIN) tissue and 22 samples of normal cervical tissue were detected by immunohistochemical method, and their correlations were analyzed.Results:The positive rates of AQP1 in cervical squamous cell carcinoma tissue and CIN tissue were 90.24% and 81.82%, respectively, which was higher than that in normal cervical tissue(40.91%)(P<0.01).The positive rates of VEGF in cervical squamous cell carcinoma tissue and CIN tissue were 100.00% and 96.36%, respectively, which was significantly higher than that in normal cervical tissue(63.64%)(P<0.01).The differences of the expressions of AQP1 and VEGF between cervical squamous cell carcinoma and CIN tissue were not statistically significant(P>0.05).The differences of the expressions of AQP1 in different ages and histological grades in cervical squamous cell carcinoma tissue were not statistically significant(P>0.05), the expression of AQP1 was significantly positive correlation with the expression of VEGF(P<0.01).Conclusions:The expressions of AQP1 and VEGF in cervical squamous cell carcinoma tissue and CIN tissue are upregulated, which may be related to the occurrence and development of the cervical squamous carcinoma.
Objective:To investigate the expressions of aquaporin1(AQP1) and vascular endothelial growth factor(VEGF) in cervical squamous cell carcinoma tissue and their correlations.Methods:The expressions of AQP1 and VEGF in 41 samples of cervical squamous cell carcinoma tissues, 55 samples of cervical intraepithelial neoplasisa(CIN) tissue and 22 samples of normal cervical tissue were detected by immunohistochemical method, and their correlations were analyzed.Results:The positive rates of AQP1 in cervical squamous cell carcinoma tissue and CIN tissue were 90.24% and 81.82%, respectively, which was higher than that in normal cervical tissue(40.91%)(P<0.01).The positive rates of VEGF in cervical squamous cell carcinoma tissue and CIN tissue were 100.00% and 96.36%, respectively, which was significantly higher than that in normal cervical tissue(63.64%)(P<0.01).The differences of the expressions of AQP1 and VEGF between cervical squamous cell carcinoma and CIN tissue were not statistically significant(P>0.05).The differences of the expressions of AQP1 in different ages and histological grades in cervical squamous cell carcinoma tissue were not statistically significant(P>0.05), the expression of AQP1 was significantly positive correlation with the expression of VEGF(P<0.01).Conclusions:The expressions of AQP1 and VEGF in cervical squamous cell carcinoma tissue and CIN tissue are upregulated, which may be related to the occurrence and development of the cervical squamous carcinoma.
2016, 41(3): 317-319.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.012
Abstract:
Objective:To investigate the effects of 40℃ 3% sodium chloride solution nasal irrigation combined with fluticasone nasal spray in the treatment of allergic rhinitis(AR).Methods:The clinical data of 160 patients with AR were retrospectively analyzed.The patients were randomly divided into the control group and treatment group(80 cases each group).The control group and treatment group were treated with fluticasone propionate nasal spray and 40℃ 3% sodium chloride solution nasal irrigation, respectively.The symptoms, signs and general condition in two groups were evaluated after 8 weeks of treatment.Results:The effective rate in treatment group was higher than that in control group(P<0.05), the nasal obstruction, rhinorrhea and sneeze in treatment group were less than those in control group(P<0.01), and the symptom differences of rhinocnesmus and throat itching between two groups were not statistically significant(P>0.05).The nasal mucosal edema and nasal secretion in treatment group were less than those in control group(P<0.05 and P<0.01), but the differences of the nasal mucosa pale and conjunctival congestion between two groups were not statistically significant(P>0.05).Conclusions:The treatment of AR with 40℃ 3% sodium chloride solution nasal irrigation combined with fluticasone propionate nasal spray is safe and effective.
Objective:To investigate the effects of 40℃ 3% sodium chloride solution nasal irrigation combined with fluticasone nasal spray in the treatment of allergic rhinitis(AR).Methods:The clinical data of 160 patients with AR were retrospectively analyzed.The patients were randomly divided into the control group and treatment group(80 cases each group).The control group and treatment group were treated with fluticasone propionate nasal spray and 40℃ 3% sodium chloride solution nasal irrigation, respectively.The symptoms, signs and general condition in two groups were evaluated after 8 weeks of treatment.Results:The effective rate in treatment group was higher than that in control group(P<0.05), the nasal obstruction, rhinorrhea and sneeze in treatment group were less than those in control group(P<0.01), and the symptom differences of rhinocnesmus and throat itching between two groups were not statistically significant(P>0.05).The nasal mucosal edema and nasal secretion in treatment group were less than those in control group(P<0.05 and P<0.01), but the differences of the nasal mucosa pale and conjunctival congestion between two groups were not statistically significant(P>0.05).Conclusions:The treatment of AR with 40℃ 3% sodium chloride solution nasal irrigation combined with fluticasone propionate nasal spray is safe and effective.
2016, 41(3): 320-322.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.013
Abstract:
Objective:To investigate the clinical efficacy of Fufangkushen injection combined with chemotherapy in the treatment of advanced lung adenocarcinoma.Methods:Eighty-seven patients with advanced lung adenocarcinoma were randomly divided into the Fufangkushen injection combined with chemotherapy group(observation group, 44 cases) and chemotherapy alone group(control group, 43 cases).The effective rate, progression-free survival and quality of life in two groups were observed.Results:The total effective rates in observation group and control group were 36.4% and 30.2%, respectively, the difference of which was not statistically significant(P>0.05).The difference of the progression-free survival between two groups was not statistically significant(P>0.05).The score of quality of life in observation group was better than that in control group(P<0.01).The incidence of adverse reaction in observation group was lower than that in control group(P<0.05 to P<0.01).Conclusions:Fufangkushen injection combined with chemotherapy treatment can not improve treatment response and prolong survival time, but which can improve the quality of life and alleviate the adverse reaction of chemotherapy.
Objective:To investigate the clinical efficacy of Fufangkushen injection combined with chemotherapy in the treatment of advanced lung adenocarcinoma.Methods:Eighty-seven patients with advanced lung adenocarcinoma were randomly divided into the Fufangkushen injection combined with chemotherapy group(observation group, 44 cases) and chemotherapy alone group(control group, 43 cases).The effective rate, progression-free survival and quality of life in two groups were observed.Results:The total effective rates in observation group and control group were 36.4% and 30.2%, respectively, the difference of which was not statistically significant(P>0.05).The difference of the progression-free survival between two groups was not statistically significant(P>0.05).The score of quality of life in observation group was better than that in control group(P<0.01).The incidence of adverse reaction in observation group was lower than that in control group(P<0.05 to P<0.01).Conclusions:Fufangkushen injection combined with chemotherapy treatment can not improve treatment response and prolong survival time, but which can improve the quality of life and alleviate the adverse reaction of chemotherapy.
2016, 41(3): 323-324,328.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.014
Abstract:
Objective:To investigate the effects of reduced glutathione on acute exacerbation of chronic obstructive pulmonary disease(AECOPD) therapy.Methods:Seventy AECOPD patients were randomly divided into the control group and observation group(35 cases each group).The levels of 8-iso-prostaglandin-F2α(8-iso-PGF2α) and C reactive protein(CRP), pulmonary function and blood gas analysis in two groups were detected at the time of admission.Two groups were treated with conventional anti-inflammatory, expectorant and antiasthmatic for 1week, the observation group were additionally treated with reduced glutathione for 1 week.The levels of 8-iso-prostaglandin-F2α and CRP, pulmonary function and blood gas analysis in two groups were detected again after 1 week of treatment, and statistically analyzed.Results:Compared with pre-treatment, the levels of 8-iso-PGF2α and CRP in two groups decreased significantly after 1 week of treatment(P<0.01), the levels of 8-iso-PGF2α and CRP in observation group were significantly lower than those in control group(P<0.01).Compared with pre-treatment, the indexes of pulmonary function and blood gas analysis in two groups were significantly improved after treatment, the improvement degree in observation group were significantly better than those in control group(P<0.01).Conclusions:Reduced glutathione can alleviate the oxidative stress response in patients with AECOPD, which has a certain adjuvant therapy value.
Objective:To investigate the effects of reduced glutathione on acute exacerbation of chronic obstructive pulmonary disease(AECOPD) therapy.Methods:Seventy AECOPD patients were randomly divided into the control group and observation group(35 cases each group).The levels of 8-iso-prostaglandin-F2α(8-iso-PGF2α) and C reactive protein(CRP), pulmonary function and blood gas analysis in two groups were detected at the time of admission.Two groups were treated with conventional anti-inflammatory, expectorant and antiasthmatic for 1week, the observation group were additionally treated with reduced glutathione for 1 week.The levels of 8-iso-prostaglandin-F2α and CRP, pulmonary function and blood gas analysis in two groups were detected again after 1 week of treatment, and statistically analyzed.Results:Compared with pre-treatment, the levels of 8-iso-PGF2α and CRP in two groups decreased significantly after 1 week of treatment(P<0.01), the levels of 8-iso-PGF2α and CRP in observation group were significantly lower than those in control group(P<0.01).Compared with pre-treatment, the indexes of pulmonary function and blood gas analysis in two groups were significantly improved after treatment, the improvement degree in observation group were significantly better than those in control group(P<0.01).Conclusions:Reduced glutathione can alleviate the oxidative stress response in patients with AECOPD, which has a certain adjuvant therapy value.
2016, 41(3): 325-328.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.015
Abstract:
Objective:To explore the expression of microRNA(miR)-506 in cervical cancer and its clinically pathological significance.Methods:The expressions of miR-506 in cervical cancer tissues and corresponding paracarcinoma tissues in 46 patients were detected using stem ring real-time reverse transcription polymerase chain reaction, the clinically pathological data were analyzed according to the miR-506 expression situation.The migration and invasion of Hela cells transfected with miR-506 were detected using Transwell, and the E-cadherin and vimentin protein expressions were detected using Western Blot.Results:The expression of MiR-506 in cervical cancer tissue was significantly lower than that in paracarcinoma tissues(P<0.01).The expression of MiR-506 in type Ⅲ/Ⅳ cervical cancer tissue was significantly lower than that in type Ⅰ/Ⅱ cervical cancer tissue(P<0.01).The expression of MiR-506 in low differentiation cervical cancer tissue was significantly lower than that in high differentiation cervical cancer tissue(P<0.01).The expression of MiR-506 in patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis(P<0.01).The differences of the MiR-506 expressions in different ages, menostasis and tumor diameters were not statistically significant(P>0.05).The migration and invasion of Hela cells transfected with miR-506 was significantly lower than those in Hela cells non-transfected with miR-506(P<0.01).The expressions of vimentin and E-cadherin in Hela cells transfected with miR-506 were inhibited and promoted, respectively(P<0.01).Conclusions:The expression of miR-506 in cervical cancer is low, which may be associated with tumor growth and development of cervical cancer, the miR-506 has certain value in judging the prognosis of cervical cancer.
Objective:To explore the expression of microRNA(miR)-506 in cervical cancer and its clinically pathological significance.Methods:The expressions of miR-506 in cervical cancer tissues and corresponding paracarcinoma tissues in 46 patients were detected using stem ring real-time reverse transcription polymerase chain reaction, the clinically pathological data were analyzed according to the miR-506 expression situation.The migration and invasion of Hela cells transfected with miR-506 were detected using Transwell, and the E-cadherin and vimentin protein expressions were detected using Western Blot.Results:The expression of MiR-506 in cervical cancer tissue was significantly lower than that in paracarcinoma tissues(P<0.01).The expression of MiR-506 in type Ⅲ/Ⅳ cervical cancer tissue was significantly lower than that in type Ⅰ/Ⅱ cervical cancer tissue(P<0.01).The expression of MiR-506 in low differentiation cervical cancer tissue was significantly lower than that in high differentiation cervical cancer tissue(P<0.01).The expression of MiR-506 in patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis(P<0.01).The differences of the MiR-506 expressions in different ages, menostasis and tumor diameters were not statistically significant(P>0.05).The migration and invasion of Hela cells transfected with miR-506 was significantly lower than those in Hela cells non-transfected with miR-506(P<0.01).The expressions of vimentin and E-cadherin in Hela cells transfected with miR-506 were inhibited and promoted, respectively(P<0.01).Conclusions:The expression of miR-506 in cervical cancer is low, which may be associated with tumor growth and development of cervical cancer, the miR-506 has certain value in judging the prognosis of cervical cancer.
2016, 41(3): 329-332.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.016
Abstract:
Objective:To analyze the clinical efficacy of total glucosides paeony(TGP) combined with methorexate(MTX) in treatment of rheumatoid arthritis.Methods:Randomized controlled trials about the effect of TGP combined with MTX on patients were collected by searching PubMed, China National Knowledge Infrastructure, China Biology Medicine disc and Wan Fang Database.The quality of included studies was assessed according to the criteria recommended Cochrane Collaboration, Meta-analysis was performed using RevMan5.3 software.Results:Ten randomized controlled trials involving 867 patients were included, 484 in experimental group, and 383 in control group.Meta-analysis results were shown that the effective rate of experimental group was better than that of control group after 12 and 24 weeks(P<0.01).Nevertheless, there were no statistical significance about the effective rate between experimental group and control group after 4 and 8 weeks(P>0.05).Conclusions:TGP combined with MTX in the treatment of rheumatoid arthritis is much better than MTX alone in total effective rate.
Objective:To analyze the clinical efficacy of total glucosides paeony(TGP) combined with methorexate(MTX) in treatment of rheumatoid arthritis.Methods:Randomized controlled trials about the effect of TGP combined with MTX on patients were collected by searching PubMed, China National Knowledge Infrastructure, China Biology Medicine disc and Wan Fang Database.The quality of included studies was assessed according to the criteria recommended Cochrane Collaboration, Meta-analysis was performed using RevMan5.3 software.Results:Ten randomized controlled trials involving 867 patients were included, 484 in experimental group, and 383 in control group.Meta-analysis results were shown that the effective rate of experimental group was better than that of control group after 12 and 24 weeks(P<0.01).Nevertheless, there were no statistical significance about the effective rate between experimental group and control group after 4 and 8 weeks(P>0.05).Conclusions:TGP combined with MTX in the treatment of rheumatoid arthritis is much better than MTX alone in total effective rate.
2016, 41(3): 333-336.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.017
Abstract:
Objective:To investigate the influencing factors of the severity of coronary artery disease(CAD).Methods:Two hundred and sixty-five CAD patients and 64 normal people diagnosed by coronary angiography were invetigated.The risk factors of coronary disease, included male, age, hypertension, diabetes mellitus, hyperlipemia, lower serum high density lipoprotein cholesterol(HDL-C), hyperuricemia, family history and smoking, were recorded.The severity of CAD, number of disease vessels and scores of Gensini were determined by coronary angiography.The relationship between risk factor and severity of CAD was analyzed using the univariate analysis and multivariate logistic regression.Results:The univariate analysis showed that the age, male, hypertension, diabetes mellitus, smoking and lower HDL-C were associated with the severity of CAD, the age, fasting blood glucose, serum uric acid, LDL-C were postive correlation with Gensini scores(P<0.05 to P<0.01), the HDL-C was negatively correlated to Gensini scores(P<0.05), and the age, systolic pressure and serum uric acid were postively correlated to the number of diseased vessels(P<0.05 to P<0.01).Multivariate linear regression analysis showed that male was the most risk factor in severity of CAD, and followed by age, hypertension and diabetes mellitus(standandized coefficients B for 0.279, 0.189, 0.164 and 0.116, respectively).Logistic regression analysis showed that the age, male, hypertension and diabetes mellitus were the independent risk factors for CAD, and the Odds Ratio(OR) value of diabetes mellitus and male were higher(OR=5.789 and OR=5.516), followed by hypertension(OR=2.407) and age(OR=2.093)(P<0.05 to P<0.01).Conclusions:The age, male, hypertension, diabetes mellitus are the main influencing factors on CAD, and the hypertension and diabetes mellitus should be prevented and controled.
Objective:To investigate the influencing factors of the severity of coronary artery disease(CAD).Methods:Two hundred and sixty-five CAD patients and 64 normal people diagnosed by coronary angiography were invetigated.The risk factors of coronary disease, included male, age, hypertension, diabetes mellitus, hyperlipemia, lower serum high density lipoprotein cholesterol(HDL-C), hyperuricemia, family history and smoking, were recorded.The severity of CAD, number of disease vessels and scores of Gensini were determined by coronary angiography.The relationship between risk factor and severity of CAD was analyzed using the univariate analysis and multivariate logistic regression.Results:The univariate analysis showed that the age, male, hypertension, diabetes mellitus, smoking and lower HDL-C were associated with the severity of CAD, the age, fasting blood glucose, serum uric acid, LDL-C were postive correlation with Gensini scores(P<0.05 to P<0.01), the HDL-C was negatively correlated to Gensini scores(P<0.05), and the age, systolic pressure and serum uric acid were postively correlated to the number of diseased vessels(P<0.05 to P<0.01).Multivariate linear regression analysis showed that male was the most risk factor in severity of CAD, and followed by age, hypertension and diabetes mellitus(standandized coefficients B for 0.279, 0.189, 0.164 and 0.116, respectively).Logistic regression analysis showed that the age, male, hypertension and diabetes mellitus were the independent risk factors for CAD, and the Odds Ratio(OR) value of diabetes mellitus and male were higher(OR=5.789 and OR=5.516), followed by hypertension(OR=2.407) and age(OR=2.093)(P<0.05 to P<0.01).Conclusions:The age, male, hypertension, diabetes mellitus are the main influencing factors on CAD, and the hypertension and diabetes mellitus should be prevented and controled.
2016, 41(3): 337-339.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.018
Abstract:
Objective:To observe the clinical efficacy of the atorvastatin combined with clopidogrel and aspirin in the treatment of carotid arterial sclerosis in patients with progressive cerebral infarction.Methods:Fifty-five progressive cerebral infarction patients with carotid arterial sclerosis were randomly divided into the control group and observation group(28 cases each group).The control group were treated with simvastatin(20 mg/d) combined with aspirin(300 mg/d), once a day by oral before sleeping.The observation group were treated with aspirin(100 mg/d) combined with atorvastatin(40 mg/d) and clopidogrel(75 mg/d), once a day by oral before sleeping.Two groups were treated for 6 months.The carotid intima-media thickness(IMT), maximal thickness of atheromatous plaque, and area and number of plaque were observed using Doppler ultrasound before and after treatment.Results:The carotid IMT, maximal thickness of atheromatous plaque, and area of plaque in two groups decreased significantly after treatment(P<0.01), the differences of whose were statistically significant(P<0.05 to P<0.01).After treatment, the number of unstable plaque and stable plaque in control group was almost unchange.15 patches of unstable plaque and 9 patches of stable plaque in observation group decreased, and the differences of whose were statistically significant(P<0.01).Conclusions:The treatment of carotid arterial sclerosis in patients with progressive cerebral infarction with atorvastatin combined with clopidogrel and aspirin can reduce the carotid IMT, maximal thickness and area of plaque, and play a positive role in stabilizing therosclerotic plaque.
Objective:To observe the clinical efficacy of the atorvastatin combined with clopidogrel and aspirin in the treatment of carotid arterial sclerosis in patients with progressive cerebral infarction.Methods:Fifty-five progressive cerebral infarction patients with carotid arterial sclerosis were randomly divided into the control group and observation group(28 cases each group).The control group were treated with simvastatin(20 mg/d) combined with aspirin(300 mg/d), once a day by oral before sleeping.The observation group were treated with aspirin(100 mg/d) combined with atorvastatin(40 mg/d) and clopidogrel(75 mg/d), once a day by oral before sleeping.Two groups were treated for 6 months.The carotid intima-media thickness(IMT), maximal thickness of atheromatous plaque, and area and number of plaque were observed using Doppler ultrasound before and after treatment.Results:The carotid IMT, maximal thickness of atheromatous plaque, and area of plaque in two groups decreased significantly after treatment(P<0.01), the differences of whose were statistically significant(P<0.05 to P<0.01).After treatment, the number of unstable plaque and stable plaque in control group was almost unchange.15 patches of unstable plaque and 9 patches of stable plaque in observation group decreased, and the differences of whose were statistically significant(P<0.01).Conclusions:The treatment of carotid arterial sclerosis in patients with progressive cerebral infarction with atorvastatin combined with clopidogrel and aspirin can reduce the carotid IMT, maximal thickness and area of plaque, and play a positive role in stabilizing therosclerotic plaque.
2016, 41(3): 340-342.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.019
Abstract:
Objective:To investigate the clinical effects of fluor varnish on preventing the dental caries in children.Methods:Seventy children were randomly divided into the control group and observation group(38 cases each group, 80 teeth).The dental caries in control group and observation group were prevented using fissure sealant and fluor varnish combined with fissure sealant, respectively.All cases were followed up for 1 to 2 years, the occurrence of dental caries and sealant falling in two groups were observed.Results:The differences of the occurrences of dental caries and interproximal caries between two groups were not statistical significance(P>0.05).The mean of caries and sealant falling in observation group were lower than those in control group(P<0.01).Conclusions:The clinical effect of fluoride varnish combined with fissure sealant on protecting the dental caries in children are good, and fluor protector can effectively protect fissure sealant, avoid its loss and strengthen preventive effects.
Objective:To investigate the clinical effects of fluor varnish on preventing the dental caries in children.Methods:Seventy children were randomly divided into the control group and observation group(38 cases each group, 80 teeth).The dental caries in control group and observation group were prevented using fissure sealant and fluor varnish combined with fissure sealant, respectively.All cases were followed up for 1 to 2 years, the occurrence of dental caries and sealant falling in two groups were observed.Results:The differences of the occurrences of dental caries and interproximal caries between two groups were not statistical significance(P>0.05).The mean of caries and sealant falling in observation group were lower than those in control group(P<0.01).Conclusions:The clinical effect of fluoride varnish combined with fissure sealant on protecting the dental caries in children are good, and fluor protector can effectively protect fissure sealant, avoid its loss and strengthen preventive effects.
2016, 41(3): 342-344.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.020
Abstract:
Objective:To investigate the effects of subclinical hypothyroidism complicated with anemia on puerpera and neonate, and its preventive measures.Methods:Four hundred and one pregnant women were divided into the subclinical hypothyroidism(161 cases, group Ⅰ), subclinical hypothyroidism complicated with anemia(82 cases, group Ⅱ), and normal puerpera(158 cases, group Ⅲ) according to the thyroid function and blood routine level.The incidences of postpartum hemorrhage, cesarean section, oligohydramnios, amniotic fluid contamination, fetal distress and neonatal asphyxia and small gestation age between three groups were compared.Results:The incidences of cesarean section in group Ⅰ and Ⅱ, and amniotic fluid pollution and fetal distress in group Ⅱ were higher than those in group Ⅲ(P<0.05).There were no statistical difference in other indexes between three groups(P>0.05).Conclusions:The subclinical hypothyroidism complicated anemia has adverse effects on pregnancy outcome, the prevention of which should be strengthened.
Objective:To investigate the effects of subclinical hypothyroidism complicated with anemia on puerpera and neonate, and its preventive measures.Methods:Four hundred and one pregnant women were divided into the subclinical hypothyroidism(161 cases, group Ⅰ), subclinical hypothyroidism complicated with anemia(82 cases, group Ⅱ), and normal puerpera(158 cases, group Ⅲ) according to the thyroid function and blood routine level.The incidences of postpartum hemorrhage, cesarean section, oligohydramnios, amniotic fluid contamination, fetal distress and neonatal asphyxia and small gestation age between three groups were compared.Results:The incidences of cesarean section in group Ⅰ and Ⅱ, and amniotic fluid pollution and fetal distress in group Ⅱ were higher than those in group Ⅲ(P<0.05).There were no statistical difference in other indexes between three groups(P>0.05).Conclusions:The subclinical hypothyroidism complicated anemia has adverse effects on pregnancy outcome, the prevention of which should be strengthened.
2016, 41(3): 345-347,350.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.021
Abstract:
Objective:To investigate the effects of the Xingnaojing injection on coagulation and nerve function in patients with acute cerebral infarction.Methods:Ninety patients with acute cerebral infarction were randomly divided into the observation group and control group(45 cases each group).The control group were treated with routine method, the observation group were additionally treated with Xingnaojing injection based on routine method.Results:The number of CD34+ cell and white blood cell in the observation group were the highest after 6 days of treatment, which was obviously higher than those at other time-points(P<0.01).The differences of platelet count, and levels of alanine aminotransferase, serum creatinine and D-dimer in observation group at all time-points were not statistically significant(P>0.05).The differences of activated partial thromboplastin time, prothrombin time and fibrinogen level in observation group at different time-points were statistically significant(P<0.01).The NIHSS scores in observation group after 2 and 3 months of treatment were significantly lower than those in control group(P<0.01).Conclusions:The Xingnaojing injection can effectively regulate the coagulation and nerve function, and improve the short-term effects in patients with acute cerebral infarction.
Objective:To investigate the effects of the Xingnaojing injection on coagulation and nerve function in patients with acute cerebral infarction.Methods:Ninety patients with acute cerebral infarction were randomly divided into the observation group and control group(45 cases each group).The control group were treated with routine method, the observation group were additionally treated with Xingnaojing injection based on routine method.Results:The number of CD34+ cell and white blood cell in the observation group were the highest after 6 days of treatment, which was obviously higher than those at other time-points(P<0.01).The differences of platelet count, and levels of alanine aminotransferase, serum creatinine and D-dimer in observation group at all time-points were not statistically significant(P>0.05).The differences of activated partial thromboplastin time, prothrombin time and fibrinogen level in observation group at different time-points were statistically significant(P<0.01).The NIHSS scores in observation group after 2 and 3 months of treatment were significantly lower than those in control group(P<0.01).Conclusions:The Xingnaojing injection can effectively regulate the coagulation and nerve function, and improve the short-term effects in patients with acute cerebral infarction.
2016, 41(3): 348-350.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.022
Abstract:
Objective:To study the clinical application value of transurethral resection of the prostate(TURP) combined with stone gravel and clearance system in the treatment of benign prostate hyperplasia(BPH) complicated with bladder calculi.Methods:The gravel time, TURP time, operation time, postoperative ambulation time, stone-free rate, intraoperative and postoperative complications, postoperative international prostate symptom score(IPSS) and quality of life(QOL) score in 82 BPH complicated with bladder calculi patients treated with TURP combined with stone gravel and clearance system were analyzed.Results:The operations in 82 patients were successfully completed.The gravel time, TURP time, total operation time and postoperative ambulation time in 82 cases were(22.7±13.6)minutes, (55.3±20.6)minutes, (87.2±21.5) minutes and(4.3±1.5)days, respectively.The postoperative bladder calculi stone-free rate was 100%.No transurethral resection syndrome, obturator nerve reflex, bladder perforation and massive haemorrhage leading to open surgery were found.The postoperative IPSS and QOL score were significantly lower than those in before operation(P<0.01).Conclusions:The treatment of BPH complicated with bladder calculi with TURP combined with stone gravel and clearance system is sfe and effective.
Objective:To study the clinical application value of transurethral resection of the prostate(TURP) combined with stone gravel and clearance system in the treatment of benign prostate hyperplasia(BPH) complicated with bladder calculi.Methods:The gravel time, TURP time, operation time, postoperative ambulation time, stone-free rate, intraoperative and postoperative complications, postoperative international prostate symptom score(IPSS) and quality of life(QOL) score in 82 BPH complicated with bladder calculi patients treated with TURP combined with stone gravel and clearance system were analyzed.Results:The operations in 82 patients were successfully completed.The gravel time, TURP time, total operation time and postoperative ambulation time in 82 cases were(22.7±13.6)minutes, (55.3±20.6)minutes, (87.2±21.5) minutes and(4.3±1.5)days, respectively.The postoperative bladder calculi stone-free rate was 100%.No transurethral resection syndrome, obturator nerve reflex, bladder perforation and massive haemorrhage leading to open surgery were found.The postoperative IPSS and QOL score were significantly lower than those in before operation(P<0.01).Conclusions:The treatment of BPH complicated with bladder calculi with TURP combined with stone gravel and clearance system is sfe and effective.
2016, 41(3): 355-356,360.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.025
Abstract:
Objective:To explore the effects of preventive use of antimicrobial agents on improving type Ⅰ incision by modified prescription comment.Methods:The medical records of type Ⅰ incision surgery were randomly selected.The rationality of preventive use of antimicrobial agents was contrastively analyzed by the before and after modified method of prescription comment.Results:Using the modified prescription comment, the utilization rate of the preventive use of antimicrobial agents in type Ⅰ incision decreased from 48.3% to 31.6%(P<0.01), the reasonable use rate of antimicrobial drug increased from 57.5% to 92%(P<0.01), and the reasonable use rate of the preventive use of antibiotics in perioperative type Ⅰ incision increased from 74.5% to 87.3%(P<0.01).Conclusions:The modified prescription comment can significantly decrease the utilization rate and increase the reasonable rate of the preventive use of antimicrobial agents in type Ⅰ incision, which plays the active role in ensuring medication safety and improving the consciousness of use drug.
Objective:To explore the effects of preventive use of antimicrobial agents on improving type Ⅰ incision by modified prescription comment.Methods:The medical records of type Ⅰ incision surgery were randomly selected.The rationality of preventive use of antimicrobial agents was contrastively analyzed by the before and after modified method of prescription comment.Results:Using the modified prescription comment, the utilization rate of the preventive use of antimicrobial agents in type Ⅰ incision decreased from 48.3% to 31.6%(P<0.01), the reasonable use rate of antimicrobial drug increased from 57.5% to 92%(P<0.01), and the reasonable use rate of the preventive use of antibiotics in perioperative type Ⅰ incision increased from 74.5% to 87.3%(P<0.01).Conclusions:The modified prescription comment can significantly decrease the utilization rate and increase the reasonable rate of the preventive use of antimicrobial agents in type Ⅰ incision, which plays the active role in ensuring medication safety and improving the consciousness of use drug.
2016, 41(3): 357-360.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.026
Abstract:
Objective:To investigate the cognition and application of antibiotics in vocational college students, analyze the risk factors of the abuse of antibiotics, and provide the scientific evidence for developing health education of antibiotics.Methods:The students from two vocational schools in Suzhou were investigated using the cluster sampling method.The investigation content included the profession, grade, cognition of antibiotics, use of antibiotics and purchasing prescription antibiotics.The differences of the cognition and use of antibiotics between two school students were compared by the cognitive scores and percentage of each indicator.Results:Four thousand four hundred and sixty-two effective questionnaires were collected, which included 2543 medical students and 1919 nonmedical students.Among 4462 students, 3621 students(81.2%) prepared for the antibiotic.One thousand and fifteen medical students(40%) and 666 nonmedical students(34.7%) with similar symptom took it by themselves.One thousand one hundred and forty-four medical students(45.0%) and 943 nonmedical students(49.1%) read the instruction carefully before using the antibiotic.Seven hundred and one medical students(27.6%) and 276 nonmedical students(14.4%) knew that the licensed pharmacist must be equipped in community pharmacy according to the state law.One thousand five hundred and sixty-eight medical students(61.7%) and 1063 nonmedical students(55.4%) thought that they were never asked to show the prescription when they bought the antibiotics, the difference between medical student and nonmedical student was statistically significant(P<0.01).Conclusions:The low antibiotic cognition and abuse of antibiotics in vocational students is common.The different cognition of antibiotic between medical student and nonmedical student results from their different knowledge.
Objective:To investigate the cognition and application of antibiotics in vocational college students, analyze the risk factors of the abuse of antibiotics, and provide the scientific evidence for developing health education of antibiotics.Methods:The students from two vocational schools in Suzhou were investigated using the cluster sampling method.The investigation content included the profession, grade, cognition of antibiotics, use of antibiotics and purchasing prescription antibiotics.The differences of the cognition and use of antibiotics between two school students were compared by the cognitive scores and percentage of each indicator.Results:Four thousand four hundred and sixty-two effective questionnaires were collected, which included 2543 medical students and 1919 nonmedical students.Among 4462 students, 3621 students(81.2%) prepared for the antibiotic.One thousand and fifteen medical students(40%) and 666 nonmedical students(34.7%) with similar symptom took it by themselves.One thousand one hundred and forty-four medical students(45.0%) and 943 nonmedical students(49.1%) read the instruction carefully before using the antibiotic.Seven hundred and one medical students(27.6%) and 276 nonmedical students(14.4%) knew that the licensed pharmacist must be equipped in community pharmacy according to the state law.One thousand five hundred and sixty-eight medical students(61.7%) and 1063 nonmedical students(55.4%) thought that they were never asked to show the prescription when they bought the antibiotics, the difference between medical student and nonmedical student was statistically significant(P<0.01).Conclusions:The low antibiotic cognition and abuse of antibiotics in vocational students is common.The different cognition of antibiotic between medical student and nonmedical student results from their different knowledge.
2016, 41(3): 361-364.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.027
Abstract:
Objective:To investigate the sanitary situation of rural drinking water and its influencing factor in Lu'an city, and provide scientific basis in improving water quality and ensuring the safety of rural drinking water.Methods:The 1154 samples of rural drinking water in Lu'an city from 2013 to 2014 were evaluated, and the influencing factors of the quality of rural drinking water were analyzed using univariate and multivariate methods.Results:The total qualified rate in two years was 34.32%, the qualified rate in 2013(39.30%) was higher than that in 2014 year(31.92%)(P<0.05).Univariate analysis showed that the year, region, type of water source, water treatment, use of water disinfection facilities, disinfectant and operating year of water supply engineering were the influence factors of quality of rural drinking water(P<0.05 to P<0.01).Multivariate analysis showed that the year, region, water treatment and operating year of water supply engineering was the independent influencing factor of quality of rural drinking water(P<0.05 to P<0.01).Conclusions:The rural drinking water in Lu'an city is low qualified rate, high microbial index, imperfection infrastructure and disinfection facility.Strengthening the protection of water source, supply facilities, and hygienic supervision should be implemented in rural drinking water.
Objective:To investigate the sanitary situation of rural drinking water and its influencing factor in Lu'an city, and provide scientific basis in improving water quality and ensuring the safety of rural drinking water.Methods:The 1154 samples of rural drinking water in Lu'an city from 2013 to 2014 were evaluated, and the influencing factors of the quality of rural drinking water were analyzed using univariate and multivariate methods.Results:The total qualified rate in two years was 34.32%, the qualified rate in 2013(39.30%) was higher than that in 2014 year(31.92%)(P<0.05).Univariate analysis showed that the year, region, type of water source, water treatment, use of water disinfection facilities, disinfectant and operating year of water supply engineering were the influence factors of quality of rural drinking water(P<0.05 to P<0.01).Multivariate analysis showed that the year, region, water treatment and operating year of water supply engineering was the independent influencing factor of quality of rural drinking water(P<0.05 to P<0.01).Conclusions:The rural drinking water in Lu'an city is low qualified rate, high microbial index, imperfection infrastructure and disinfection facility.Strengthening the protection of water source, supply facilities, and hygienic supervision should be implemented in rural drinking water.
2016, 41(3): 372-373,376.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.030
Abstract:
Objective:To explore the application value of power doppler ultrasound in estimating the tocolysis effects of threatened abortion.Methods:One hundred and forty-seven threatened abortion patients and 150 normal pregnant women were set as observation group and control group, respectively.The observation group were treated with progesterone tocolysis by intramuscular injection, the progesterone level and power doppler ultrasound in observation group were detected at the first day and after 1 week of admission.The relevant examination index and success rate of tocolysis between two groups were compared.Results:The VI, FI and VFI in observation group at the first day and after 1 week of admission were significantly higher than those in control group, and the increasing extent of which in observation group were significantly higher than that in control group(P<0.01).After tocolysis treatment in observation group, cure in 99 cases, good effect in 37 cases and invalid in 11 cases were identified, and the total effective rate was 92.52%.Conclusions:The power doppler ultrasound can accurately assess the placental blood perfusion, auxiliarily diagnosed and treated the threatened abortion and evaluate treatment effect.
Objective:To explore the application value of power doppler ultrasound in estimating the tocolysis effects of threatened abortion.Methods:One hundred and forty-seven threatened abortion patients and 150 normal pregnant women were set as observation group and control group, respectively.The observation group were treated with progesterone tocolysis by intramuscular injection, the progesterone level and power doppler ultrasound in observation group were detected at the first day and after 1 week of admission.The relevant examination index and success rate of tocolysis between two groups were compared.Results:The VI, FI and VFI in observation group at the first day and after 1 week of admission were significantly higher than those in control group, and the increasing extent of which in observation group were significantly higher than that in control group(P<0.01).After tocolysis treatment in observation group, cure in 99 cases, good effect in 37 cases and invalid in 11 cases were identified, and the total effective rate was 92.52%.Conclusions:The power doppler ultrasound can accurately assess the placental blood perfusion, auxiliarily diagnosed and treated the threatened abortion and evaluate treatment effect.
2016, 41(3): 374-376.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.031
Abstract:
Objective:To measure the liver shear wave velocity(SWV) in hepatolenticular degeneration(HLD) patients by virtual touch tissue quantification(VTQ) technology, evaluate the texture of the liver, and explore the correlations of liver SWV with copper biochemical indicators and serum liver fibrosis indexes.Methods:The SWV value in HLD patients was recorded by Siemens ACUSON-S2000 color doppler ultrasonic diagnostic instrument, which was equipped with the acoustic radiation force impulse imaging software and VTQ technology.The copper biochemical indicators including copper ion(Cu2+), ceruloplasmin(CP), and copper oxidase(SCO) and serum liver fibrosis indexes including procollagen type Ⅲ(PC-Ⅲ), collagen type Ⅳ(Ⅳ-C), laminin(LN) and hyaluronic acid(HA) were measured.The correlation of the liver SWV value with Cu2+, CP, SCO, PC-Ⅲ, Ⅳ-C, LN and HA were analyzed.Results:The liver SWV value was not correlation with Cu2+ and CP(P>0.05), negative correlation with SCO value(P<0.05).The SWV value was positive correlation with serum liver fibrosis indexes(PC-Ⅲ, Ⅳ-C, LN and HA)(P<0.05 to P<0.01).Conclusions:The ultrasound VTQ technology can reflect the Cu2+ transport activity and liver fibrosis degree to a certain extent, which has high clinical application value in the diagnosis of HLD.
Objective:To measure the liver shear wave velocity(SWV) in hepatolenticular degeneration(HLD) patients by virtual touch tissue quantification(VTQ) technology, evaluate the texture of the liver, and explore the correlations of liver SWV with copper biochemical indicators and serum liver fibrosis indexes.Methods:The SWV value in HLD patients was recorded by Siemens ACUSON-S2000 color doppler ultrasonic diagnostic instrument, which was equipped with the acoustic radiation force impulse imaging software and VTQ technology.The copper biochemical indicators including copper ion(Cu2+), ceruloplasmin(CP), and copper oxidase(SCO) and serum liver fibrosis indexes including procollagen type Ⅲ(PC-Ⅲ), collagen type Ⅳ(Ⅳ-C), laminin(LN) and hyaluronic acid(HA) were measured.The correlation of the liver SWV value with Cu2+, CP, SCO, PC-Ⅲ, Ⅳ-C, LN and HA were analyzed.Results:The liver SWV value was not correlation with Cu2+ and CP(P>0.05), negative correlation with SCO value(P<0.05).The SWV value was positive correlation with serum liver fibrosis indexes(PC-Ⅲ, Ⅳ-C, LN and HA)(P<0.05 to P<0.01).Conclusions:The ultrasound VTQ technology can reflect the Cu2+ transport activity and liver fibrosis degree to a certain extent, which has high clinical application value in the diagnosis of HLD.
2016, 41(3): 377-379.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.032
Abstract:
Objective:To explore the correlation of the serum total cholesterol level with mortality in elderly male patients with coronary heart disease.Methods:Two hundred and eight elderly male patients with coronary heart disease were divided into the group A(serum total cholesterol <4.16 mmol/L), group B(4.16 mmol/L≤serum total cholesterol <4.68 mmol/L), group C(4.68 mmol/L≤serum total cholesterol <5.20 mmol/L) and group D(serum total cholesterol >5.20 mmol/L) according to the serum total cholesterol levels.The serum triglyceride level, physical examination, age, body mass index and mortality in all patients were analyzed.Results:The differences of the serum total cholesterol levels between four groups were statistically significant(P<0.01).The mortalities in group A and D were higher than that in group B, and the mortality in group D was higher than that in group C(P<0.05).The differences of the mortalities in group A & group D and group B & group C were not statistically significant(P>0.05).The differences of age, body mass index and triglyceride level between four groups were not statistically significant(P>0.05).The mortality in elderly male coronary heart disease patients with serum total cholesterol levels <4.16 mmol/L or >5.20 mmol/L increased.Conclusions:The serum total cholesterol level in elderly male patients with coronary heart disease is closely correlated to the mortality.The high or low serum total cholesterol levels are the risk factors of the death of the elderly male patients with coronary heart disease.
Objective:To explore the correlation of the serum total cholesterol level with mortality in elderly male patients with coronary heart disease.Methods:Two hundred and eight elderly male patients with coronary heart disease were divided into the group A(serum total cholesterol <4.16 mmol/L), group B(4.16 mmol/L≤serum total cholesterol <4.68 mmol/L), group C(4.68 mmol/L≤serum total cholesterol <5.20 mmol/L) and group D(serum total cholesterol >5.20 mmol/L) according to the serum total cholesterol levels.The serum triglyceride level, physical examination, age, body mass index and mortality in all patients were analyzed.Results:The differences of the serum total cholesterol levels between four groups were statistically significant(P<0.01).The mortalities in group A and D were higher than that in group B, and the mortality in group D was higher than that in group C(P<0.05).The differences of the mortalities in group A & group D and group B & group C were not statistically significant(P>0.05).The differences of age, body mass index and triglyceride level between four groups were not statistically significant(P>0.05).The mortality in elderly male coronary heart disease patients with serum total cholesterol levels <4.16 mmol/L or >5.20 mmol/L increased.Conclusions:The serum total cholesterol level in elderly male patients with coronary heart disease is closely correlated to the mortality.The high or low serum total cholesterol levels are the risk factors of the death of the elderly male patients with coronary heart disease.
2016, 41(3): 380-382.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.033
Abstract:
Objective:To analyze the resistant characteristics of ESBLs Escherichia coli produced by urine culture, investigate its antibacterial activity in vitro and compare with other antibacterial drugs.Methods:A total of 821 strains were cultured from the strain library, the ESBLs Escherichia coli produced by urine culture were screened according to the phenotype of ESBLs, the antibacterial activities in vitro of 15 antimicrobial agents, including fosfomycin, were detected by K-B method.Results:Among the 821Escherichia coli strains, the sensitivities of the 465 ESBLs Escherichia coli strains produced by urine culture to imipenem were high(91.4%), following by fosfomycin(88.0%), the sensitivities of which to levofloxacin, penicillin and cephalosporin were low.Conclusions:The sensitivity of carbapenem is the highest drug, the antibacterial activity in vitro of fosfomycin to ESBLs Escherichia coli produced by urine culture is good, which has certain clinical value.
Objective:To analyze the resistant characteristics of ESBLs Escherichia coli produced by urine culture, investigate its antibacterial activity in vitro and compare with other antibacterial drugs.Methods:A total of 821 strains were cultured from the strain library, the ESBLs Escherichia coli produced by urine culture were screened according to the phenotype of ESBLs, the antibacterial activities in vitro of 15 antimicrobial agents, including fosfomycin, were detected by K-B method.Results:Among the 821Escherichia coli strains, the sensitivities of the 465 ESBLs Escherichia coli strains produced by urine culture to imipenem were high(91.4%), following by fosfomycin(88.0%), the sensitivities of which to levofloxacin, penicillin and cephalosporin were low.Conclusions:The sensitivity of carbapenem is the highest drug, the antibacterial activity in vitro of fosfomycin to ESBLs Escherichia coli produced by urine culture is good, which has certain clinical value.
2016, 41(3): 383-386.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.034
Abstract:
Objective:To investigate the current status of nurse-patient communication ability in nursing intern undergraduates, and analyze its influencing factors.Methods:The emotional intelligence, empathy and nurse-patient communication abilities in 505 nursing intern undergraduates were investigated using emotional intelligence ability scale, empathy ability scale and nurse-patient communication ability scale.Results:The total scores of the emotional intelligence, empathy and nurse-patient communication abilities were(126.77±13.12), (112.35±12.96) and(52.55±7.45), respectively.The family relationship, professional attitude and frequency of participating activity in nursing intern undergraduates were the influencing factors of nurse-patient communication ability(P<0.05 to P<0.01).The nurse-patient communication abilities in nursing intern undergraduates were positive correlation with emotional intelligence and empathy abilities(P<0.01).Conclusions:The nurse-patient communication abilities in nursing intern undergraduates with good family relationship, active professional attitude and high frequency of participating activity are high.The higher the emotional intelligence and empathy abilities are, the higher the nurse-patient communication abilities of nursing intern undergraduates are.
Objective:To investigate the current status of nurse-patient communication ability in nursing intern undergraduates, and analyze its influencing factors.Methods:The emotional intelligence, empathy and nurse-patient communication abilities in 505 nursing intern undergraduates were investigated using emotional intelligence ability scale, empathy ability scale and nurse-patient communication ability scale.Results:The total scores of the emotional intelligence, empathy and nurse-patient communication abilities were(126.77±13.12), (112.35±12.96) and(52.55±7.45), respectively.The family relationship, professional attitude and frequency of participating activity in nursing intern undergraduates were the influencing factors of nurse-patient communication ability(P<0.05 to P<0.01).The nurse-patient communication abilities in nursing intern undergraduates were positive correlation with emotional intelligence and empathy abilities(P<0.01).Conclusions:The nurse-patient communication abilities in nursing intern undergraduates with good family relationship, active professional attitude and high frequency of participating activity are high.The higher the emotional intelligence and empathy abilities are, the higher the nurse-patient communication abilities of nursing intern undergraduates are.
2016, 41(3): 387-391.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.035
Abstract:
Objective:To investigate the status of posttraumatic growth (PTG) and analyze its influencing factors among patients with myocardial infarction so as to provide basis for improving the PTG in myocardial infarction patients.Methods:A total of 172 patients with myocardial infarction were surveyed by filling out the social-demographic and disease-related information forms, the Life Events Scale and the Posttraumatic Growth Inventory by convenience sampling.Results:Different levels of PTG occurred in 78.5% of the patients with myocardial infarction, with a total score of 69.09±13.88.The total score of PTG was 69.09±13.88.Each dimension score of PTG from high to low in order was as follows:appreciation of life(21.78±4.34), new possibilities(13.65±3.21), self-transformation(13.52±3.34), relation with others(10.56±2.44) and personal ability(9.78±2.19).Single-factor analysis showed that the career of the patients, duration of the disease, monthly family income, religious faith, knowledge about the disease and whether or not combined with other diseases affected the PTG score significantly(P<0.01).Multiple-factor analysis showed that whether or not combined with other diseases, knowledge about the disease and monthly family income finally entered into the regression equation and accounted for 34.6 percent of the PTG.Conclusions:The essential predictors include whether or not the patients are combined with other diseases, the patients' knowledge about the disease and their monthly family income.
Objective:To investigate the status of posttraumatic growth (PTG) and analyze its influencing factors among patients with myocardial infarction so as to provide basis for improving the PTG in myocardial infarction patients.Methods:A total of 172 patients with myocardial infarction were surveyed by filling out the social-demographic and disease-related information forms, the Life Events Scale and the Posttraumatic Growth Inventory by convenience sampling.Results:Different levels of PTG occurred in 78.5% of the patients with myocardial infarction, with a total score of 69.09±13.88.The total score of PTG was 69.09±13.88.Each dimension score of PTG from high to low in order was as follows:appreciation of life(21.78±4.34), new possibilities(13.65±3.21), self-transformation(13.52±3.34), relation with others(10.56±2.44) and personal ability(9.78±2.19).Single-factor analysis showed that the career of the patients, duration of the disease, monthly family income, religious faith, knowledge about the disease and whether or not combined with other diseases affected the PTG score significantly(P<0.01).Multiple-factor analysis showed that whether or not combined with other diseases, knowledge about the disease and monthly family income finally entered into the regression equation and accounted for 34.6 percent of the PTG.Conclusions:The essential predictors include whether or not the patients are combined with other diseases, the patients' knowledge about the disease and their monthly family income.
2016, 41(3): 392-394.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.036
Abstract:
Objective:To investigate the self-care ability and its influencing factors in hemodialysis patients.Methods:The self-care ability and its influencing factors in 195 hemodialysis patients were investigated using the self-care ability scale(ESCA).Results:The total score of self-care ability in 195 hemodialysis patients was (134.77±29.02), which was at a higher level.There was higher score of self-care ability in patients with hemodialysis who were of vocational school and above education, married or divorced than those who were of primary school and below education, widowed, respectively(P<0.01~P<0.05).The multiple linear regression analysis showed that the education degree was the main influencing factor on the self-care ability.Conclusions:According to the affecting factors of self-care ability in hemodialysis patients, some effective measures should be taken to further improve the self-care ability.
Objective:To investigate the self-care ability and its influencing factors in hemodialysis patients.Methods:The self-care ability and its influencing factors in 195 hemodialysis patients were investigated using the self-care ability scale(ESCA).Results:The total score of self-care ability in 195 hemodialysis patients was (134.77±29.02), which was at a higher level.There was higher score of self-care ability in patients with hemodialysis who were of vocational school and above education, married or divorced than those who were of primary school and below education, widowed, respectively(P<0.01~P<0.05).The multiple linear regression analysis showed that the education degree was the main influencing factor on the self-care ability.Conclusions:According to the affecting factors of self-care ability in hemodialysis patients, some effective measures should be taken to further improve the self-care ability.
2016, 41(3): 395-397.
doi: 10.13898/j.cnki.issn.1000-2200.2016.03.037
Abstract:
Objective:To investigate the effects of the doctor-nurse-pharmacist integration mode on the compliance of patients treated with hepatitis B.antiviral drug by oral.Methods:One hundred and twenty-five patients with poor medication compliance were screened from 200 chronic hepatitis B patients with 25 to 55 years old, and randomly divided into the intervention group(63 cases) and control group(62 cases).The intervention group were nursed with the doctor-nurse-pharmacist integration mode(including psychology guidance and health education), the control group were nursed with general nursing measures.Results:The compliance of hepatitis B.antiviral drug by oral in intervention group was obviously better than that in control group(P<0.05).Conclusions:The doctor-nurse-pharmacist integration mode can improve the cognitive level of compliance, antiviral treatment compliance and effects of antiviral therapy in hepatitis B patients.
Objective:To investigate the effects of the doctor-nurse-pharmacist integration mode on the compliance of patients treated with hepatitis B.antiviral drug by oral.Methods:One hundred and twenty-five patients with poor medication compliance were screened from 200 chronic hepatitis B patients with 25 to 55 years old, and randomly divided into the intervention group(63 cases) and control group(62 cases).The intervention group were nursed with the doctor-nurse-pharmacist integration mode(including psychology guidance and health education), the control group were nursed with general nursing measures.Results:The compliance of hepatitis B.antiviral drug by oral in intervention group was obviously better than that in control group(P<0.05).Conclusions:The doctor-nurse-pharmacist integration mode can improve the cognitive level of compliance, antiviral treatment compliance and effects of antiviral therapy in hepatitis B patients.
Email
RSS