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2018 Vol. 43, No. 2
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2018, 43(2): 141-145.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.001
Abstract:
Objective: To establish hepatocellular carcinoma cell line SMMC-7721/ADM resistance to adriamycin,and explore the mechanism of microRNA(miR)-143 in regulating the cell line resistance to adriamycin.Methods: The cell line SMMC-7721/ADM resistance to adriamycin was constructed using the gradual increasing the concentration of adriamycin,and the IC50 value of which was evaluated.The mRNA expression levels of miR-143, DNA methyltransferase 3B(DNMT3B) and multidrug resistance gene 1(MDR1),Bax and Bcl-2 genes were evaluated using real-time fluorescent quantitative PCR,and the protein level of DNMT3B was determined by Western blotting.After the miR-143 mimics upregulating the miR-143 expression in SMMC-7721/ADM cell line,the IC50 value,invasion ability,cell apoptosis,and levels of DNMT3B,MDR1 were detected.The miR-143 mimics was transiently transfected into the drug resistance cell line using liposome-2000,and the indexes changes were observed.Results: The cell line SMMC-7721/ADM resistance to adriamycin was successfully established,the IC50 value raised 78.97 times(P<0.01).The expression level of miR-143 decreased,and the expression levels of DNMT3B,MDR1,Bax and Bcl-2 increased,and the differences of which were statistically significant(P<0.05 to P<0.01).After the miR-143 mimics transfecting into the drug resistance cell line,the IC50 value reduced 57%,the invasion ability declined,the cell apoptosis increased,the levels of DNMT3B and MDR1 mRNA decreased,and the differences of which were statistically significant(P<0.01).Conclusions: The increasing sensitivity of hepatocellular carcinoma to adriamycin may be caused by the miR-143 reducing DNMT3B expression.
Objective: To establish hepatocellular carcinoma cell line SMMC-7721/ADM resistance to adriamycin,and explore the mechanism of microRNA(miR)-143 in regulating the cell line resistance to adriamycin.Methods: The cell line SMMC-7721/ADM resistance to adriamycin was constructed using the gradual increasing the concentration of adriamycin,and the IC50 value of which was evaluated.The mRNA expression levels of miR-143, DNA methyltransferase 3B(DNMT3B) and multidrug resistance gene 1(MDR1),Bax and Bcl-2 genes were evaluated using real-time fluorescent quantitative PCR,and the protein level of DNMT3B was determined by Western blotting.After the miR-143 mimics upregulating the miR-143 expression in SMMC-7721/ADM cell line,the IC50 value,invasion ability,cell apoptosis,and levels of DNMT3B,MDR1 were detected.The miR-143 mimics was transiently transfected into the drug resistance cell line using liposome-2000,and the indexes changes were observed.Results: The cell line SMMC-7721/ADM resistance to adriamycin was successfully established,the IC50 value raised 78.97 times(P<0.01).The expression level of miR-143 decreased,and the expression levels of DNMT3B,MDR1,Bax and Bcl-2 increased,and the differences of which were statistically significant(P<0.05 to P<0.01).After the miR-143 mimics transfecting into the drug resistance cell line,the IC50 value reduced 57%,the invasion ability declined,the cell apoptosis increased,the levels of DNMT3B and MDR1 mRNA decreased,and the differences of which were statistically significant(P<0.01).Conclusions: The increasing sensitivity of hepatocellular carcinoma to adriamycin may be caused by the miR-143 reducing DNMT3B expression.
2018, 43(2): 146-149.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.002
Abstract:
Objective: To investigate the comprehensive effects of norepinephrine(NE) and angiotensin Ⅱ(AngⅡ) on tyrosine phosphorylation of insulin receptor β(Ins-Rβ) subunit and insulin receptor substance 1(IRS-1) in rat cardiac cells,and the mechanism of insulin resistance.Methods: The myocardial cells from the neonatal rat(1 to 3d) were primary cultured,and co-cultured with the same concentration of the mixture of NE and AngⅡ for 72 h according to the concentration gradient.The protein expressions of insulin signal molecules were detected using Western blotting.Losartan,Ang Ⅱ receptor blockers,was added into each experiment group,and the independent effect of NE and AngⅡ was observed.Results: Under the same concentration of the mixture of NE and Ang Ⅱ,the protein expression levels of Ins-R,IRS-1 and glucose transporter type 4(GLUT4),and level of tyrosine phosphorylation of Ins-Rβ decreased,and the level of tyrosine phosphorylation of IRS-1 increased in myocardial cells(P<0.05).The effect of 1 μmol/L of losartan on the level of tyrosine phosphorylation of Ins-Rβ was not obvious(P>0.05),which could decrease the level of tyrosine phosphorylation of IRS-1(P<0.05).Conclusions: The same concentration of the mixture of NE and AngⅡ can decrease the tyrosine phosphorylation level of Ins-R β subunit,and increase the tyrosine phosphorylation level of IRS-1.NE is the crucial factor in decreasing the tyrosine phosphorylation level of Ins-Rβ,and the AngⅡ is the crucial factor in increasing the tyrosine phosphorylation level of IRS-1.
Objective: To investigate the comprehensive effects of norepinephrine(NE) and angiotensin Ⅱ(AngⅡ) on tyrosine phosphorylation of insulin receptor β(Ins-Rβ) subunit and insulin receptor substance 1(IRS-1) in rat cardiac cells,and the mechanism of insulin resistance.Methods: The myocardial cells from the neonatal rat(1 to 3d) were primary cultured,and co-cultured with the same concentration of the mixture of NE and AngⅡ for 72 h according to the concentration gradient.The protein expressions of insulin signal molecules were detected using Western blotting.Losartan,Ang Ⅱ receptor blockers,was added into each experiment group,and the independent effect of NE and AngⅡ was observed.Results: Under the same concentration of the mixture of NE and Ang Ⅱ,the protein expression levels of Ins-R,IRS-1 and glucose transporter type 4(GLUT4),and level of tyrosine phosphorylation of Ins-Rβ decreased,and the level of tyrosine phosphorylation of IRS-1 increased in myocardial cells(P<0.05).The effect of 1 μmol/L of losartan on the level of tyrosine phosphorylation of Ins-Rβ was not obvious(P>0.05),which could decrease the level of tyrosine phosphorylation of IRS-1(P<0.05).Conclusions: The same concentration of the mixture of NE and AngⅡ can decrease the tyrosine phosphorylation level of Ins-R β subunit,and increase the tyrosine phosphorylation level of IRS-1.NE is the crucial factor in decreasing the tyrosine phosphorylation level of Ins-Rβ,and the AngⅡ is the crucial factor in increasing the tyrosine phosphorylation level of IRS-1.
2018, 43(2): 149-151.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.003
Abstract:
Objective: To explore the effects of different doses of formaldehyde on the teratosperm rate,bone marrow micronucleus rate,level of serum testosterone and testicular tissue in male Wistar rats.Methods: Forty sexual maturity male Wistar rats were randomly divided into the negative control group(treated with normal saline),low-dose formaldehyde poisoning group(treated with 0.2 mg·kg-1·d-1),mid-dose formaldehyde poisoning group(treated with 2 mg·kg-1·d-1),high-dose formaldehyde poisoning group(treated with 20 mg·kg-1·d-1),and positive control group(treated with 50 mg·kg-1·d-1 cyclophosphamide).The levels of serum testosterone in rats were measured after 1 week of intraperitoneal injection.After 2 days of poisoning,the eyeball was removed,the blood sample was collected,and all rats were sacrificed by cervical dislocation method.The teratosperm rate and micronucleus rate in all rats were analyzed.The testicle morphology and histopathology were analyzed by paraffin embedded section.Results: Compared with the negative control group,the teratosperm rate in low-and mid-dose formaldehyde poisoning groups increased(P<0.05),and the increasing degree of which in high-dose formaldehyde poisoning group and positive control group was more obvious(P<0.01).Compared with the negative control group,the increasing degree of micronucleus rate in low-and mid-dose formaldehyde poisoning groups was not obvious(P>0.05),and the increasing degree of which in high-dose formaldehyde poisoning group and positive control group was more obvious(P<0.01).Compared with the negative control group,the levels of serum testosterone in low-and mid-dose formaldehyde poisoning groups decreased(P<0.01),and the decreasing degree of which in high-dose formaldehyde poisoning group and positive control group was more obvious(P<0.01).The paraffin embedded testicle section showed the tissue injury in high-dose formaldehyde poisoning group was severe.Conclusions: The formaldehyde can lead to the sperm malformation,increase the bone marrow micronucleus rate,and decrease the levels of serum testosterone in male Wistar rats.The formaldehyde has certain reproductive and genetic toxicity of in male Wistar rats.
Objective: To explore the effects of different doses of formaldehyde on the teratosperm rate,bone marrow micronucleus rate,level of serum testosterone and testicular tissue in male Wistar rats.Methods: Forty sexual maturity male Wistar rats were randomly divided into the negative control group(treated with normal saline),low-dose formaldehyde poisoning group(treated with 0.2 mg·kg-1·d-1),mid-dose formaldehyde poisoning group(treated with 2 mg·kg-1·d-1),high-dose formaldehyde poisoning group(treated with 20 mg·kg-1·d-1),and positive control group(treated with 50 mg·kg-1·d-1 cyclophosphamide).The levels of serum testosterone in rats were measured after 1 week of intraperitoneal injection.After 2 days of poisoning,the eyeball was removed,the blood sample was collected,and all rats were sacrificed by cervical dislocation method.The teratosperm rate and micronucleus rate in all rats were analyzed.The testicle morphology and histopathology were analyzed by paraffin embedded section.Results: Compared with the negative control group,the teratosperm rate in low-and mid-dose formaldehyde poisoning groups increased(P<0.05),and the increasing degree of which in high-dose formaldehyde poisoning group and positive control group was more obvious(P<0.01).Compared with the negative control group,the increasing degree of micronucleus rate in low-and mid-dose formaldehyde poisoning groups was not obvious(P>0.05),and the increasing degree of which in high-dose formaldehyde poisoning group and positive control group was more obvious(P<0.01).Compared with the negative control group,the levels of serum testosterone in low-and mid-dose formaldehyde poisoning groups decreased(P<0.01),and the decreasing degree of which in high-dose formaldehyde poisoning group and positive control group was more obvious(P<0.01).The paraffin embedded testicle section showed the tissue injury in high-dose formaldehyde poisoning group was severe.Conclusions: The formaldehyde can lead to the sperm malformation,increase the bone marrow micronucleus rate,and decrease the levels of serum testosterone in male Wistar rats.The formaldehyde has certain reproductive and genetic toxicity of in male Wistar rats.
2018, 43(2): 152-155,159.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.004
Abstract:
Objective: To explore the effects of ethanol extract of psoralen-nutmeg on aquaporin(AQP) and cytokines in spleen deficiency diarrhea mice.Methods: Sixty Kunming mice were randomly divided into the normal group,model group,Shenling Baizhu powder group,and low,medium and high dose of ethanol extract of psoralen-nutmeg group(10 mice each group).The spleen deficiency diarrhea mice in model group,Shenling Baizhu powder group and ethanol extract group were established using rhubarb,and the normal group was gavaged using the same volume distilled water.After successful modeling,the low,medium and high dose of ethanol extract group were gavaged with 7.02,14.04 and 21.06 g/kg of ethanol extract of psoralen-nutmeg,respectively.The Shenling Baizhu powder group was gavaged with Shenling Baizhu powder at the dose of 2.34 g/kg,and the normal group and model group were gavaged with the same volume of distilled water,once a week,for 2 weeks.The body mass,thymus index and spleen index between all groups were compared.The protein expression levels of AQP3 and AQP4 were detected by immunohistochemistry,and the levels of IL-1β、IL-2、TNF-α of colon were measured using ELISA.Results: The difference of the body mass between all groups was statistically significant(P<0.01),which in model group was significantly lower than that in normal group(P<0.01),and which in medium and high dose of ethanol extract group was higher than that in model group(P<0.05).The thymus index and spleen index in model group were significantly lower than those in normal group(P<0.05 and P<0.01),and which in middle and high dose of ethanol extract group were higher than those in model group(P<0.05).The results of immunohistochemistry showed that the protein expression levels of AQP3 and AQP4 in normal group and ethanol extract group were lower than those in normal group(P<0.05 to P<0.01),and the protein expression levels of AQP3 and AQP4 in medium and high dose of ethanol extract group and Shenling Baizhu powder group were significantly higher than those in model group and low dose of ethanol extract group(P<0.01).Compared with normal group,the levels of IL-1β,IL-2 and TNF-α in colon tissue in model group significantly increased(P<0.01).Compared with model group and low and medium dose of ethanol extract group,the levels of IL-1β,IL-2 and TNF-α in colon tissue in high dose of ethanol extract group and Shenling Baizhu powder group significantly decreased(P<0.01).Conclusions: Ethanol extract of psoralen-nutmeg can obviously enhance the expression of aquaporin,reduce the levels of intestinal inflammatory factors,and improve the gastrointestinal function of spleen deficiency diarrhea mice.
Objective: To explore the effects of ethanol extract of psoralen-nutmeg on aquaporin(AQP) and cytokines in spleen deficiency diarrhea mice.Methods: Sixty Kunming mice were randomly divided into the normal group,model group,Shenling Baizhu powder group,and low,medium and high dose of ethanol extract of psoralen-nutmeg group(10 mice each group).The spleen deficiency diarrhea mice in model group,Shenling Baizhu powder group and ethanol extract group were established using rhubarb,and the normal group was gavaged using the same volume distilled water.After successful modeling,the low,medium and high dose of ethanol extract group were gavaged with 7.02,14.04 and 21.06 g/kg of ethanol extract of psoralen-nutmeg,respectively.The Shenling Baizhu powder group was gavaged with Shenling Baizhu powder at the dose of 2.34 g/kg,and the normal group and model group were gavaged with the same volume of distilled water,once a week,for 2 weeks.The body mass,thymus index and spleen index between all groups were compared.The protein expression levels of AQP3 and AQP4 were detected by immunohistochemistry,and the levels of IL-1β、IL-2、TNF-α of colon were measured using ELISA.Results: The difference of the body mass between all groups was statistically significant(P<0.01),which in model group was significantly lower than that in normal group(P<0.01),and which in medium and high dose of ethanol extract group was higher than that in model group(P<0.05).The thymus index and spleen index in model group were significantly lower than those in normal group(P<0.05 and P<0.01),and which in middle and high dose of ethanol extract group were higher than those in model group(P<0.05).The results of immunohistochemistry showed that the protein expression levels of AQP3 and AQP4 in normal group and ethanol extract group were lower than those in normal group(P<0.05 to P<0.01),and the protein expression levels of AQP3 and AQP4 in medium and high dose of ethanol extract group and Shenling Baizhu powder group were significantly higher than those in model group and low dose of ethanol extract group(P<0.01).Compared with normal group,the levels of IL-1β,IL-2 and TNF-α in colon tissue in model group significantly increased(P<0.01).Compared with model group and low and medium dose of ethanol extract group,the levels of IL-1β,IL-2 and TNF-α in colon tissue in high dose of ethanol extract group and Shenling Baizhu powder group significantly decreased(P<0.01).Conclusions: Ethanol extract of psoralen-nutmeg can obviously enhance the expression of aquaporin,reduce the levels of intestinal inflammatory factors,and improve the gastrointestinal function of spleen deficiency diarrhea mice.
2018, 43(2): 156-159.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.005
Abstract:
Objective: To establish the type 2 diabetes mellitus(T2DM) rat model,and observe the effects of pioglitazone on insulin resistance and function of pancreatic islets in T2DM rats.Methods: Forty SD male rats were randomly divided into the control group(NC group,10 rats) and high-fat group(30 rats).Twenty-four T2DM model rats were established,and randomly divided into the diabetes mellitus group(DM group) and PIO group(12 rats in each group).The levels of body mass(BM),fasting blood glucose(FBG),fasting insulin(FINS),glycosylated hemoglobin hemoglobin-A1c(HbA1c),C-reactive protein(CRP),blood lipid,insulin resistance index,islet β-cell function index and insulin sensitivity index(ISI) were measured in two groups.Results: The levels of BM,TC,TG,LDL-C,FBG,HbA1c,FINS/CRP and HOMA-β index in DM group and PIO group were higher than those in control group(P<0.05 to P<0.01).The levels of HDL-C,ISI and HOMA-β in DM group and PIO group were lower than those in control group(P<0.05 to P<0.01),and the level of HOMA-β in PIO group was higher than that in DM group(P<0.05).Conclusions: Pioglitazone plays a therapeutic role in T2DM rats,which may be involved in reducing insulin resistance and improving islet function.
Objective: To establish the type 2 diabetes mellitus(T2DM) rat model,and observe the effects of pioglitazone on insulin resistance and function of pancreatic islets in T2DM rats.Methods: Forty SD male rats were randomly divided into the control group(NC group,10 rats) and high-fat group(30 rats).Twenty-four T2DM model rats were established,and randomly divided into the diabetes mellitus group(DM group) and PIO group(12 rats in each group).The levels of body mass(BM),fasting blood glucose(FBG),fasting insulin(FINS),glycosylated hemoglobin hemoglobin-A1c(HbA1c),C-reactive protein(CRP),blood lipid,insulin resistance index,islet β-cell function index and insulin sensitivity index(ISI) were measured in two groups.Results: The levels of BM,TC,TG,LDL-C,FBG,HbA1c,FINS/CRP and HOMA-β index in DM group and PIO group were higher than those in control group(P<0.05 to P<0.01).The levels of HDL-C,ISI and HOMA-β in DM group and PIO group were lower than those in control group(P<0.05 to P<0.01),and the level of HOMA-β in PIO group was higher than that in DM group(P<0.05).Conclusions: Pioglitazone plays a therapeutic role in T2DM rats,which may be involved in reducing insulin resistance and improving islet function.
2018, 43(2): 160-163,167.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.006
Abstract:
Objective: To detect the expression level of p33ING1 in human gastric cancer tissue,and investigate the relationship between p33ING1 level and clinicopathologic characteristics.Methods: The expression levels of p33ING1 in 91 gastric cancer samples,30 normal gastric mucosa tissue samples and 35 atypical hyperplasia tissue samples were detected using immunohistochemical staining method.Results: The positive rate of p33ING1 protein in gastric carcinoma tissue was higher than that in normal gastric tissue and atypical hyperplasia tissue(P<0.01).The differences of the p33ING1 protein levels in gastric carcinoma tissue with different depth of invasion,grade of tumor,lymph node metastasis and Duke's stages were statistically significant(P<0.05).The results of Log-rank test of survival curves showed that the depth of invasion and lymph node metastasis of gastric cancer were related to prognosis,and the expression level of p33ING1 was not related to prognosis.Conclusions: The abnormal expression of p33ING1 in the gastric tissue may be associated with the development of gastric cancer,which is expected to provide a new target for the treatment of gastric cancer.
Objective: To detect the expression level of p33ING1 in human gastric cancer tissue,and investigate the relationship between p33ING1 level and clinicopathologic characteristics.Methods: The expression levels of p33ING1 in 91 gastric cancer samples,30 normal gastric mucosa tissue samples and 35 atypical hyperplasia tissue samples were detected using immunohistochemical staining method.Results: The positive rate of p33ING1 protein in gastric carcinoma tissue was higher than that in normal gastric tissue and atypical hyperplasia tissue(P<0.01).The differences of the p33ING1 protein levels in gastric carcinoma tissue with different depth of invasion,grade of tumor,lymph node metastasis and Duke's stages were statistically significant(P<0.05).The results of Log-rank test of survival curves showed that the depth of invasion and lymph node metastasis of gastric cancer were related to prognosis,and the expression level of p33ING1 was not related to prognosis.Conclusions: The abnormal expression of p33ING1 in the gastric tissue may be associated with the development of gastric cancer,which is expected to provide a new target for the treatment of gastric cancer.
2018, 43(2): 164-167.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.007
Abstract:
Objective: To investigate the influencing factor of prognosis,and effects of hemoperfusion removing the inflammatory mediators and paraquat in paraquat poisoning patients.Methods: The clinical data of 35 patients with acute paraquat poisoning were investigated.All cases were treated with conventional method combined with HA230 resin hemoperfusion.The patients were divided into the survival group and death group according to the prognosis.The paraquat concentration,levels of IL-6 and GSH-PX,and activities of MDA and SOD in two groups between before and after the first hemoperfusion were compared.The correlations of the paraquat concentration,sex,age,time of the poisoning to first time blood perfusion,time of the liver,kidney and cardiac injury and blood routine indicators with prognosis were analyzed.Results: Among 35 cases,20 cases survived,15 cases died,and the death rate was 42.9%.The differences of the time of the poisoning to first time blood perfusion,time of the liver,kidney and cardiac injury,white blood cell count and neutrophil count between two groups were statistically significant(P<0.05 to P<0.01).Compared with before hemoperfusion,the paraquat concentration in two groups after perfusion decreased significantly(P<0.01),the SOD activity increased significantly(P<0.01).The paraquat concentration in survival group before and after the first perfusion was significantly lower than that in death group,and the differences of the levels of SOD,GSH-PX,IL-6 and MDA in two groups between before and after perfusion were not statistically significant(P>0.05).Conclusions: HA230 resin hemoperfusion can effectively remove paraquat,and improve the activity of SOD.The plasma concentration of paraquat,time of the poisoning to the first perfusion,time of liver,kidney and cardiac injury,white blood cell count,neutrophil count are the prognosis influencing factors of acute paraquat poisoning.
Objective: To investigate the influencing factor of prognosis,and effects of hemoperfusion removing the inflammatory mediators and paraquat in paraquat poisoning patients.Methods: The clinical data of 35 patients with acute paraquat poisoning were investigated.All cases were treated with conventional method combined with HA230 resin hemoperfusion.The patients were divided into the survival group and death group according to the prognosis.The paraquat concentration,levels of IL-6 and GSH-PX,and activities of MDA and SOD in two groups between before and after the first hemoperfusion were compared.The correlations of the paraquat concentration,sex,age,time of the poisoning to first time blood perfusion,time of the liver,kidney and cardiac injury and blood routine indicators with prognosis were analyzed.Results: Among 35 cases,20 cases survived,15 cases died,and the death rate was 42.9%.The differences of the time of the poisoning to first time blood perfusion,time of the liver,kidney and cardiac injury,white blood cell count and neutrophil count between two groups were statistically significant(P<0.05 to P<0.01).Compared with before hemoperfusion,the paraquat concentration in two groups after perfusion decreased significantly(P<0.01),the SOD activity increased significantly(P<0.01).The paraquat concentration in survival group before and after the first perfusion was significantly lower than that in death group,and the differences of the levels of SOD,GSH-PX,IL-6 and MDA in two groups between before and after perfusion were not statistically significant(P>0.05).Conclusions: HA230 resin hemoperfusion can effectively remove paraquat,and improve the activity of SOD.The plasma concentration of paraquat,time of the poisoning to the first perfusion,time of liver,kidney and cardiac injury,white blood cell count,neutrophil count are the prognosis influencing factors of acute paraquat poisoning.
2018, 43(2): 168-169,173.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.008
Abstract:
Objective: To observe the effects of biofeedback combined with paroxetine in the treatment of depression.Methods: Sixty patients with the first onset depression were randomly divided into the observation group and control group.Two groups were treated with 20 to 40 mg of paroxetine per day,and the observation group was additionally treated with biofeedback,3 times a week for 6 weeks.The therapeutic effects in two groups were evaluated using Hamilton depression scale(HAMD) before treatment and at 1,2,4 and 6 weeks after treatment.The adverse reactions in two groups were evaluated during treatment.Results: The differences of the effective rate and adverse reaction between two groups were not statistically significant(P>0.05).Compared with before treatment,the scores of HAMD in two groups significantly decreased after 1,2,4,6 weeks of treatment(P<0.01).At 2 and 4 weeks after treatment,the scores of HAMD in observation group were lower than that in control group(P<0.05),and at other time points,the difference of the score of HAMD between two groups was not statistically significant(P>0.05).Conclusions: The biofeedback combined with paroxetine in treating depression can improve the therapeutic effects,which is safe and worthy of promotion in clinic.
Objective: To observe the effects of biofeedback combined with paroxetine in the treatment of depression.Methods: Sixty patients with the first onset depression were randomly divided into the observation group and control group.Two groups were treated with 20 to 40 mg of paroxetine per day,and the observation group was additionally treated with biofeedback,3 times a week for 6 weeks.The therapeutic effects in two groups were evaluated using Hamilton depression scale(HAMD) before treatment and at 1,2,4 and 6 weeks after treatment.The adverse reactions in two groups were evaluated during treatment.Results: The differences of the effective rate and adverse reaction between two groups were not statistically significant(P>0.05).Compared with before treatment,the scores of HAMD in two groups significantly decreased after 1,2,4,6 weeks of treatment(P<0.01).At 2 and 4 weeks after treatment,the scores of HAMD in observation group were lower than that in control group(P<0.05),and at other time points,the difference of the score of HAMD between two groups was not statistically significant(P>0.05).Conclusions: The biofeedback combined with paroxetine in treating depression can improve the therapeutic effects,which is safe and worthy of promotion in clinic.
2018, 43(2): 170-173.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.009
Abstract:
Objective: To investigate the short term efficacy of Mammotome minimally invasive surgery in the treatment of benign lesion of breast,and its effect on the beauty of breast.Methods: Eighty patients with benign breast lesions were divided into the observation group and control group according to random number table method.The observation group was treated with Mammotome minimally invasive surgery,and the control group was treated with traditional incision surgery.The treatment effects and breast appearance between two groups were compared.Results: All patients were successfully operated,a total of 156 tumor masses were resected.The results of postoperative pathology showed the 153 masses were benign,and 3 masses were breast cancer.The operative time,bleeding loss and hospitalization time in observation group were lower than those in control group(P<0.05 to P<0.01),and the differences of the incidence rate of complications and mistaken cut rates between two groups were not statistically significant(P>0.05).The scores of breast shape,incision scar,nipple-areolar,pigmentation and breast sensation in observation group after 6 months of operation were significantly higher than those in control group(P<0.01).The satisfaction rate on the appearance of breast in observation group(100.0%) was higher than that in control group(80.0%)(P<0.01).Conclusions: The effect of Mammotome minimally invasive surgery in the treatment of benign breast lesion is good,which has high satisfaction rate,low operation risk,can maximumly conserve the breast appearance,and is worthy of application in clinic.
Objective: To investigate the short term efficacy of Mammotome minimally invasive surgery in the treatment of benign lesion of breast,and its effect on the beauty of breast.Methods: Eighty patients with benign breast lesions were divided into the observation group and control group according to random number table method.The observation group was treated with Mammotome minimally invasive surgery,and the control group was treated with traditional incision surgery.The treatment effects and breast appearance between two groups were compared.Results: All patients were successfully operated,a total of 156 tumor masses were resected.The results of postoperative pathology showed the 153 masses were benign,and 3 masses were breast cancer.The operative time,bleeding loss and hospitalization time in observation group were lower than those in control group(P<0.05 to P<0.01),and the differences of the incidence rate of complications and mistaken cut rates between two groups were not statistically significant(P>0.05).The scores of breast shape,incision scar,nipple-areolar,pigmentation and breast sensation in observation group after 6 months of operation were significantly higher than those in control group(P<0.01).The satisfaction rate on the appearance of breast in observation group(100.0%) was higher than that in control group(80.0%)(P<0.01).Conclusions: The effect of Mammotome minimally invasive surgery in the treatment of benign breast lesion is good,which has high satisfaction rate,low operation risk,can maximumly conserve the breast appearance,and is worthy of application in clinic.
2018, 43(2): 174-177.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.010
Abstract:
Objective: To observe the clinical effects of arthroscopic debridement combined with rehabilitation training in the treatment of knee osteoarthritis(KOA).Methods: One hundred and thirty-eight patients with KOA were randomly divided into the control group and observation group.The control group were treated with arthroscopic debridement,and the observation group were treated with arthroscopic debridement combined with rehabilitation training.The VAS score of knee pain and Lysholm score of knee in two groups before and after treatment joint function were evaluated.Results: Compared before treatment,the scores of VAS and Lysholm in Kellgren-Lawrence X-ray level Ⅰ,Ⅱ,Ⅲ and Ⅳ patients in two groups significantly decreased and increased after 2 months of treatment,respectively(P<0.01).The scores of VAS and Lysholmin in levelⅠand Ⅱpatients in observation group were significantly lower and higher than that in control group,respectively(P<0.01).The differences of the scores of knee joint pain and function between level Ⅲ and Ⅳ patients in two groups were not statistically significant(P>0.05).The total effective rate in observation group was higher than that in control group(P<0.05).Conclusions: Arthroscopic debridement combined with rehabilitation training can significantly improve the knee joint function,and reduce the pain in patients with KOA.The postoperative rehabilitation training can further alleviate the pain,and improve the knee joint function in levelⅠand Ⅱ patients.
Objective: To observe the clinical effects of arthroscopic debridement combined with rehabilitation training in the treatment of knee osteoarthritis(KOA).Methods: One hundred and thirty-eight patients with KOA were randomly divided into the control group and observation group.The control group were treated with arthroscopic debridement,and the observation group were treated with arthroscopic debridement combined with rehabilitation training.The VAS score of knee pain and Lysholm score of knee in two groups before and after treatment joint function were evaluated.Results: Compared before treatment,the scores of VAS and Lysholm in Kellgren-Lawrence X-ray level Ⅰ,Ⅱ,Ⅲ and Ⅳ patients in two groups significantly decreased and increased after 2 months of treatment,respectively(P<0.01).The scores of VAS and Lysholmin in levelⅠand Ⅱpatients in observation group were significantly lower and higher than that in control group,respectively(P<0.01).The differences of the scores of knee joint pain and function between level Ⅲ and Ⅳ patients in two groups were not statistically significant(P>0.05).The total effective rate in observation group was higher than that in control group(P<0.05).Conclusions: Arthroscopic debridement combined with rehabilitation training can significantly improve the knee joint function,and reduce the pain in patients with KOA.The postoperative rehabilitation training can further alleviate the pain,and improve the knee joint function in levelⅠand Ⅱ patients.
2018, 43(2): 178-180,184.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.011
Abstract:
Objective: To investigate the expression levels of hepatoma-derived growth factor(HDGF) and vascular endothelial growth factor(VEGF) in hepatoma tissue,and its correlations with microvessel density(MVD) and prognosis of patients.Methods: The expression levels of HDGF,VEGF and MVD in 94 liver pathology specimens and para-carcinoma tissue of more than 2 cm distance from tumor lesions were investigated using immunohistochemstry.Results: The positive expression rates of HDGF protein and VEGF protein(62.77%,65.96%) in hepatocellular carcinoma tissue were higher than those in para-carcinoma tissue(15.96%,19.15%)(P<0.01),and the MVD in hepatocellular carcinoma tissue was higher than that in para-carcinoma tissue(P<0.01).The expression levels of HDGF and VEGF in hepatocellular carcinoma tissue were positively correlated with the MVD count(P<0.01).The 3-year survival rate in patients with positive HDGF expression(19.61%) was lower than that in patients with negative HDGF expression(47.06%)(P<0.01).The 3-year survival rate in patients with positive VEGF expression(14.81%) was lower than that in patients with negative VEGF expression(56.25%)(P<0.01).Conclusions: The expression levels of HDGF and HDGF in hepatocellular carcinoma tissue increase,and which is positively correlated with MVD.The positive expressions of HDGF and HDGF are harmful to the long-term prognosis of patients.
Objective: To investigate the expression levels of hepatoma-derived growth factor(HDGF) and vascular endothelial growth factor(VEGF) in hepatoma tissue,and its correlations with microvessel density(MVD) and prognosis of patients.Methods: The expression levels of HDGF,VEGF and MVD in 94 liver pathology specimens and para-carcinoma tissue of more than 2 cm distance from tumor lesions were investigated using immunohistochemstry.Results: The positive expression rates of HDGF protein and VEGF protein(62.77%,65.96%) in hepatocellular carcinoma tissue were higher than those in para-carcinoma tissue(15.96%,19.15%)(P<0.01),and the MVD in hepatocellular carcinoma tissue was higher than that in para-carcinoma tissue(P<0.01).The expression levels of HDGF and VEGF in hepatocellular carcinoma tissue were positively correlated with the MVD count(P<0.01).The 3-year survival rate in patients with positive HDGF expression(19.61%) was lower than that in patients with negative HDGF expression(47.06%)(P<0.01).The 3-year survival rate in patients with positive VEGF expression(14.81%) was lower than that in patients with negative VEGF expression(56.25%)(P<0.01).Conclusions: The expression levels of HDGF and HDGF in hepatocellular carcinoma tissue increase,and which is positively correlated with MVD.The positive expressions of HDGF and HDGF are harmful to the long-term prognosis of patients.
2018, 43(2): 181-184.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.012
Abstract:
Objective: To explore the level changes of interleukin-6(IL-6) and heparin sulfate(HPSE) in plasma and blister fliud after scald,and relationship between levels of IL-6,HPSE and depth and severity of the scald wound.Methods: According to the depth of scald,39 scald patients were divided into the superficial Ⅱ degree group and deep Ⅱ degree group.Among the blister specimens,superficial Ⅱ degree in 23 cases and deep Ⅱ degree in 21 cases,and superficial Ⅱ degree in 18 cases and deep Ⅱ degree in 16 cases in plasma specimens were investigated.The levels IL-6 and HPSE in plasma and blister fluid were detected using enzyme-linked immunosorbent assay(EⅡSA) after 1,3 and 5d of scald.Results: The levels of IL-6 and HPSE in blister fluid were significantly higher than those in plasma in scald patients(P<0.01).The level of IL-6 in blister fluid of patients with deep Ⅱ degree scald was significantly higher than that of patients with superficial Ⅱ degree scald patients(P<0.01),and the level of HPSE in blister fluid of patients with deep Ⅱ degree scald was significantly lower than that of patients with superficial Ⅱ degree scald patients(P<0.01 to P<0.05).Conclusions: The level of IL-6 in blister fluid at the early stage of scald is positively related to the depth and severity of scald.The more severe scald are,the higher the level of IL-6 is.The level of HPSE in blister fluid at the early stage of scald is negatively related to the time of wound healing.The bigger the scald area is,the better the healing is,and the shorter the time is.
Objective: To explore the level changes of interleukin-6(IL-6) and heparin sulfate(HPSE) in plasma and blister fliud after scald,and relationship between levels of IL-6,HPSE and depth and severity of the scald wound.Methods: According to the depth of scald,39 scald patients were divided into the superficial Ⅱ degree group and deep Ⅱ degree group.Among the blister specimens,superficial Ⅱ degree in 23 cases and deep Ⅱ degree in 21 cases,and superficial Ⅱ degree in 18 cases and deep Ⅱ degree in 16 cases in plasma specimens were investigated.The levels IL-6 and HPSE in plasma and blister fluid were detected using enzyme-linked immunosorbent assay(EⅡSA) after 1,3 and 5d of scald.Results: The levels of IL-6 and HPSE in blister fluid were significantly higher than those in plasma in scald patients(P<0.01).The level of IL-6 in blister fluid of patients with deep Ⅱ degree scald was significantly higher than that of patients with superficial Ⅱ degree scald patients(P<0.01),and the level of HPSE in blister fluid of patients with deep Ⅱ degree scald was significantly lower than that of patients with superficial Ⅱ degree scald patients(P<0.01 to P<0.05).Conclusions: The level of IL-6 in blister fluid at the early stage of scald is positively related to the depth and severity of scald.The more severe scald are,the higher the level of IL-6 is.The level of HPSE in blister fluid at the early stage of scald is negatively related to the time of wound healing.The bigger the scald area is,the better the healing is,and the shorter the time is.
2018, 43(2): 185-188.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.013
Abstract:
Objective: To analyze the effects of ambroxol hydrochloride combined with azithromycin sequential therapy on serum cytokines and co-stimulatory molecules in children with Mycoplasma pneumonia.Methods: Eighty children with Mycoplasma pneumonia were randomly divided into the observation group and control group(40 cases each group).The control group was treated with azithromycin sequential therapy,and the observation group was treated with ambroxol hydrochloride combined with azithromycin sequential therapy.The therapeutic effect between two groups was compared.The serum levels of cytokines and co-stimulatory molecules in two groups between before and after treatment were compared.Results: The total effective rate in observation group(97.5%) was higher than that in control group(82.5%)(P<0.05).The average annealing thermal time,cough disappearing time and length of hospital stay in observation group were significantly shorter than those in control group(P<0.01).The differences of the levels of IL-4、IFN-γ、IL-17、IL-18 and sB7-H3 between two groups before treatment were not statistically significant(P>0.05).Compared with before treatment,the levels of IL-4、IFN-γ、IL-17、IL-18 and sB7-H3 in two groups significantly decreased after treatment(P<0.01),and which in observation group was significantly lower than that in control group after treatment(P<0.01).Conclusions: The treatment effect of ambroxol hydrochloride combined with azithromycin sequential therapy in children with Mycoplasma pneumonia is significant,which can effectively improve the serum levels of cytokines and co-stimulatory molecules,inhibit the inflammatory reaction,and help recovery of children.
Objective: To analyze the effects of ambroxol hydrochloride combined with azithromycin sequential therapy on serum cytokines and co-stimulatory molecules in children with Mycoplasma pneumonia.Methods: Eighty children with Mycoplasma pneumonia were randomly divided into the observation group and control group(40 cases each group).The control group was treated with azithromycin sequential therapy,and the observation group was treated with ambroxol hydrochloride combined with azithromycin sequential therapy.The therapeutic effect between two groups was compared.The serum levels of cytokines and co-stimulatory molecules in two groups between before and after treatment were compared.Results: The total effective rate in observation group(97.5%) was higher than that in control group(82.5%)(P<0.05).The average annealing thermal time,cough disappearing time and length of hospital stay in observation group were significantly shorter than those in control group(P<0.01).The differences of the levels of IL-4、IFN-γ、IL-17、IL-18 and sB7-H3 between two groups before treatment were not statistically significant(P>0.05).Compared with before treatment,the levels of IL-4、IFN-γ、IL-17、IL-18 and sB7-H3 in two groups significantly decreased after treatment(P<0.01),and which in observation group was significantly lower than that in control group after treatment(P<0.01).Conclusions: The treatment effect of ambroxol hydrochloride combined with azithromycin sequential therapy in children with Mycoplasma pneumonia is significant,which can effectively improve the serum levels of cytokines and co-stimulatory molecules,inhibit the inflammatory reaction,and help recovery of children.
2018, 43(2): 188-190.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.014
Abstract:
Objective: To investigate the effects of different nutrition support methods on the postoperative recovery of elderly patients with colorectal cancer.Methods: Fity-six colorectal cancer patients with more than 65 years old were randomly divided into the control group and observation group(28 cases each group).The control group were treated with parenteral nutrition,and the observation group were treated with enteral nutrition combined with parenteral nutrition within 24 h after operation.The levels of blood urea nitrogen,serum total protein(TP),serum albumin (Alb) and plasma prealbumin before operation and postoperative 7 days between two groups were compared,and the postoperative anal exhaust time,postoperative complications,hospitalization time,and hospitalization cost between two groups were compared.Results: The postoperative TP and Alb levels in observation group were higher than those in control group(P<0.01),and the exhaust time,hospitalization time and hospitalization cost in observation group were less than those in control group(P<0.01).The difference of Pa and incidence of complication between two groups was not statistically significant(P>0.05).Conclusions: Early enteral nutrition can increase the levels of TP and Alb,quicken exhaust,shorten hospitalization time and reduce hospitalization cost of patients.
Objective: To investigate the effects of different nutrition support methods on the postoperative recovery of elderly patients with colorectal cancer.Methods: Fity-six colorectal cancer patients with more than 65 years old were randomly divided into the control group and observation group(28 cases each group).The control group were treated with parenteral nutrition,and the observation group were treated with enteral nutrition combined with parenteral nutrition within 24 h after operation.The levels of blood urea nitrogen,serum total protein(TP),serum albumin (Alb) and plasma prealbumin before operation and postoperative 7 days between two groups were compared,and the postoperative anal exhaust time,postoperative complications,hospitalization time,and hospitalization cost between two groups were compared.Results: The postoperative TP and Alb levels in observation group were higher than those in control group(P<0.01),and the exhaust time,hospitalization time and hospitalization cost in observation group were less than those in control group(P<0.01).The difference of Pa and incidence of complication between two groups was not statistically significant(P>0.05).Conclusions: Early enteral nutrition can increase the levels of TP and Alb,quicken exhaust,shorten hospitalization time and reduce hospitalization cost of patients.
2018, 43(2): 191-194,197.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.015
Abstract:
Objective: To analyze the correlations of the indexes of nutrition and inflammation with serum levels of 25-hydroxy D3 in maintenance hemodialysis(MHD) patients so as to supply the basis for clinical nutrition evaluation.Methods: The serum level of 25-hydroxy vitamin D3,and indexes of clinical nutrition and inflammation in 127 patients treated with MHD were detected.The correlations of the serum levels of 25-hydroxy vitamin D3 with indexes of nutrition and inflammation with in all patients were analyzed by the results of malnutrition-inflammation score(MIS).Results: All patients were divided into the normal serum vitamin D3 group(19 cases),insufficient vitamin D3 group(90 cases) and deficient vitamin D3group(18 cases).The results of ANOVA analysis indicated that the differences of the levels of TP,ALB,HCY,CRP and TC between three groups were statistically significant(P<0.05).The correlation analysis indicated that the level of vitamin D3 was positively correlated with the levels of TP,HB,ALB and serum calcium(P<0.05),and negatively correlated with the level of CRP(P<0.01).The results of multi-factorial linear regression analysis showed that the ALB and CRP were main influencing factor of vitamin D3.Conclusions: The incidence of 25-hydroxy vitamin D3 insufficiency or deficiency is high in MHD patients.The inflammation status and nutritional condition are closely correlated with the serum level of 25-hydroxy vitamin D3.
Objective: To analyze the correlations of the indexes of nutrition and inflammation with serum levels of 25-hydroxy D3 in maintenance hemodialysis(MHD) patients so as to supply the basis for clinical nutrition evaluation.Methods: The serum level of 25-hydroxy vitamin D3,and indexes of clinical nutrition and inflammation in 127 patients treated with MHD were detected.The correlations of the serum levels of 25-hydroxy vitamin D3 with indexes of nutrition and inflammation with in all patients were analyzed by the results of malnutrition-inflammation score(MIS).Results: All patients were divided into the normal serum vitamin D3 group(19 cases),insufficient vitamin D3 group(90 cases) and deficient vitamin D3group(18 cases).The results of ANOVA analysis indicated that the differences of the levels of TP,ALB,HCY,CRP and TC between three groups were statistically significant(P<0.05).The correlation analysis indicated that the level of vitamin D3 was positively correlated with the levels of TP,HB,ALB and serum calcium(P<0.05),and negatively correlated with the level of CRP(P<0.01).The results of multi-factorial linear regression analysis showed that the ALB and CRP were main influencing factor of vitamin D3.Conclusions: The incidence of 25-hydroxy vitamin D3 insufficiency or deficiency is high in MHD patients.The inflammation status and nutritional condition are closely correlated with the serum level of 25-hydroxy vitamin D3.
2018, 43(2): 195-197.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.016
Abstract:
Objective: To investigate the diagnostic value of electrophysiology in neuromuscular disease in patients with hyperthyroidism.Methods: The nerve conduction and lesion condition in patients with hyperthyroidism were detected using electromyograph and needle electrode electromyography(NEMG).The muscle NEMG of different locations was analyzed.Results: The abnormal detection rate of sensory conduction(47.22%) was significantly higher than that of motor conduction(22.22%)(P<0.05).When the proximal muscle slightly contracted,the incidence rate of motor unit potential duration shortening in proximal muscle(58.33%) was higher than that in distal muscle(41.67%)(P<0.05),and the difference of the amplitude decreasing and polyphase wave increasing of muscle motor unit action potential between proximal and distal muscle was not statistically significant in hyperthyroidism patients(P>0.05).Conclusions: The neuromuscular damage in patients with hyperthyroidism can be found,the abnormal sensory conduction is main,and the nerve electrophysiology detection is helpful to clinical diagnosis.
Objective: To investigate the diagnostic value of electrophysiology in neuromuscular disease in patients with hyperthyroidism.Methods: The nerve conduction and lesion condition in patients with hyperthyroidism were detected using electromyograph and needle electrode electromyography(NEMG).The muscle NEMG of different locations was analyzed.Results: The abnormal detection rate of sensory conduction(47.22%) was significantly higher than that of motor conduction(22.22%)(P<0.05).When the proximal muscle slightly contracted,the incidence rate of motor unit potential duration shortening in proximal muscle(58.33%) was higher than that in distal muscle(41.67%)(P<0.05),and the difference of the amplitude decreasing and polyphase wave increasing of muscle motor unit action potential between proximal and distal muscle was not statistically significant in hyperthyroidism patients(P>0.05).Conclusions: The neuromuscular damage in patients with hyperthyroidism can be found,the abnormal sensory conduction is main,and the nerve electrophysiology detection is helpful to clinical diagnosis.
2018, 43(2): 198-200,205.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.017
Abstract:
Objective: To investigate the clinical effects of Effect of Yishen Huoxue Tongluo Yin in the treatment of diabetic nephropathy.Methods: Eighty-four patients with diabetic nephropathy were randomly divided into the control group and observation group(42 cases each group).The control group and observation group were treated with valsartan capsule and Yishen Huoxue Tongluo Yin,respectively.The clinical effects and related indexes between two groups were compared.Results: The total effective rate in observation group(88.09%) was higher than that in control group(73.81%)(P<0.05).Before treatment,the differences of the levels of urinary albumin,24 h urinary albumin,urea nitrogen,serum creatinine and glomerular filtration rate between two groups were not statistically significant(P>0.05).After treatment,all indexes in observation group were significantly improved(P<0.01),and which in observation group was significantly better than that in control group(P<0.01).Before treatment,the differences of the levels of MCP-1,hs-CRP and TNF-α between two groups were not statistically significant(P>0.05).After treatment,the levels of MCP-1,hs-CRP and TNF-α in observation group significantly decreased compared with before treatment(P<0.01),the differences of the levels of MCP-1,hs-CRP and TNF-α in control group between before and after treatment were not statistically significant(P>0.05),and the levels of MCP-1,hs-CRP and TNF-α in observation group were lower than those in control group(P<0.05 to P<0.01).Conclusions: The treatment effect of diabetic nephropathy with Yishen Huoxue Tongluo Yin is good.
Objective: To investigate the clinical effects of Effect of Yishen Huoxue Tongluo Yin in the treatment of diabetic nephropathy.Methods: Eighty-four patients with diabetic nephropathy were randomly divided into the control group and observation group(42 cases each group).The control group and observation group were treated with valsartan capsule and Yishen Huoxue Tongluo Yin,respectively.The clinical effects and related indexes between two groups were compared.Results: The total effective rate in observation group(88.09%) was higher than that in control group(73.81%)(P<0.05).Before treatment,the differences of the levels of urinary albumin,24 h urinary albumin,urea nitrogen,serum creatinine and glomerular filtration rate between two groups were not statistically significant(P>0.05).After treatment,all indexes in observation group were significantly improved(P<0.01),and which in observation group was significantly better than that in control group(P<0.01).Before treatment,the differences of the levels of MCP-1,hs-CRP and TNF-α between two groups were not statistically significant(P>0.05).After treatment,the levels of MCP-1,hs-CRP and TNF-α in observation group significantly decreased compared with before treatment(P<0.01),the differences of the levels of MCP-1,hs-CRP and TNF-α in control group between before and after treatment were not statistically significant(P>0.05),and the levels of MCP-1,hs-CRP and TNF-α in observation group were lower than those in control group(P<0.05 to P<0.01).Conclusions: The treatment effect of diabetic nephropathy with Yishen Huoxue Tongluo Yin is good.
2018, 43(2): 201-205.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.018
Abstract:
Objective: To explore the neuroprotective effects of Angong Niuhuang pill in severe traumatic brain injury(STBI) patients,and its effect on the permeability of enteric mucosa.Methods: One hundred and eighty patients with STBI were randomly divided into the control group and observation group(90 cases each group).The control group was treated with conventional drug,and the observation group was additionally treated with Angong Niuhuang pill based on the control group.The glasgow coma scale score,serum levels of neuron-specific enolase,IL-6 and diamine oxidase,and lactulose excretion rate between two groups before treatment and after 5 and 10 days of treatment were compared.The clinical effects and incidence rate of complication were analyzed.Results: The total effective rate in observation group(95.6%) was higher than that in control group(80.0%)(P<0.05).After 5 and 10 days of treatment,the scores of glasgow coma scale in observation group were significantly higher than those in control group(P<0.05),and the serum levels of neuron-specific enolase,IL-6 and diamine oxidase,and lactulose excretion rate in observation group were significantly lower than those in control group(P<0.01).The incidence rate of complications in observation group after treatment was significantly lower than that in control group(P<0.05 to P<0.01).Conclusions: Angong Niuhuang pill can effectively decrease the levels of neuron-specific enolase and IL-6,inhibit the inflammatory reaction,improve the permeability of enteric mucosa and promote the function recovery of the injured nerve and enteric mucosa in STBI patients.
Objective: To explore the neuroprotective effects of Angong Niuhuang pill in severe traumatic brain injury(STBI) patients,and its effect on the permeability of enteric mucosa.Methods: One hundred and eighty patients with STBI were randomly divided into the control group and observation group(90 cases each group).The control group was treated with conventional drug,and the observation group was additionally treated with Angong Niuhuang pill based on the control group.The glasgow coma scale score,serum levels of neuron-specific enolase,IL-6 and diamine oxidase,and lactulose excretion rate between two groups before treatment and after 5 and 10 days of treatment were compared.The clinical effects and incidence rate of complication were analyzed.Results: The total effective rate in observation group(95.6%) was higher than that in control group(80.0%)(P<0.05).After 5 and 10 days of treatment,the scores of glasgow coma scale in observation group were significantly higher than those in control group(P<0.05),and the serum levels of neuron-specific enolase,IL-6 and diamine oxidase,and lactulose excretion rate in observation group were significantly lower than those in control group(P<0.01).The incidence rate of complications in observation group after treatment was significantly lower than that in control group(P<0.05 to P<0.01).Conclusions: Angong Niuhuang pill can effectively decrease the levels of neuron-specific enolase and IL-6,inhibit the inflammatory reaction,improve the permeability of enteric mucosa and promote the function recovery of the injured nerve and enteric mucosa in STBI patients.
2018, 43(2): 206-209.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.019
Abstract:
Objective: To investigate the clinical effects of three kinds of mechanical ventilation modes in the treatment of severe thoracic injury complicated with acute respiratory distress syndrome(ARDS).Methods: Ninety elderly patients with severe thoracic injury complicated with ARDS were treated with mechanical ventilation by breathing machine,and three different ventilation modes were implemented according to the patient's situation.Thirty patients were treated with synchronous intermittent mandatory ventilation(SIMV) combined with positive end expiratory pressure(PEEP) (SIMV+PEEP group),30 patients were treated with continuous airway ventilation(CPAP) combined with pressure support(PSV)(CPAP+PSV group),and 30 cases were treated with pressure controlled ventilation(PCV)(PCV group).The clinical effects,respiratory mode parameters and blood gas analysis before and after treatment between three groups were compared.Results: The differences of the clinical effects between three groups were not statistically significant(P>0.05),one case in SIMV+PEEP group and one case in CPAP+PSV group and no case in PCV group died.The levels of Minvol(MV),alveolar oxygen partial pressure(PaO2) and arterial oxygen saturation(SaO2) in CPAP+PSV group were higher than those in SIMV+PEEP group and PCV group(P<0.05 to P<0.01),while the levels of proximal interphalangeal(PIP) and partial pressure of carbon dioxide in artery(PaCO2) in CPAP+PSV group were lower than those in SIMV+PEEP group and PCV group(P<0.05 to P<0.01).Compared with before treatment,the PaO2,PaCO2,SaO2 and PaO2/FiO2(fraction of inspiration O2) in 3 groups were significantly improved after treatment(P<0.01).The PaO2/FiO2 in CPAP+PSV group was higher than that in SIMV+PEEP group and PCV group(P<0.01 and P<0.05).Conclusions: All three kinds of mechanical ventilation modes can improve the hypoxia in elderly patients with severe thoracic injury complicated with ARDS.The CPAP+PSV mode has advantage over the another two ventilation modes in reducing the peak inspiratory pressure,which is more suitable for the treatment of flail chest.
Objective: To investigate the clinical effects of three kinds of mechanical ventilation modes in the treatment of severe thoracic injury complicated with acute respiratory distress syndrome(ARDS).Methods: Ninety elderly patients with severe thoracic injury complicated with ARDS were treated with mechanical ventilation by breathing machine,and three different ventilation modes were implemented according to the patient's situation.Thirty patients were treated with synchronous intermittent mandatory ventilation(SIMV) combined with positive end expiratory pressure(PEEP) (SIMV+PEEP group),30 patients were treated with continuous airway ventilation(CPAP) combined with pressure support(PSV)(CPAP+PSV group),and 30 cases were treated with pressure controlled ventilation(PCV)(PCV group).The clinical effects,respiratory mode parameters and blood gas analysis before and after treatment between three groups were compared.Results: The differences of the clinical effects between three groups were not statistically significant(P>0.05),one case in SIMV+PEEP group and one case in CPAP+PSV group and no case in PCV group died.The levels of Minvol(MV),alveolar oxygen partial pressure(PaO2) and arterial oxygen saturation(SaO2) in CPAP+PSV group were higher than those in SIMV+PEEP group and PCV group(P<0.05 to P<0.01),while the levels of proximal interphalangeal(PIP) and partial pressure of carbon dioxide in artery(PaCO2) in CPAP+PSV group were lower than those in SIMV+PEEP group and PCV group(P<0.05 to P<0.01).Compared with before treatment,the PaO2,PaCO2,SaO2 and PaO2/FiO2(fraction of inspiration O2) in 3 groups were significantly improved after treatment(P<0.01).The PaO2/FiO2 in CPAP+PSV group was higher than that in SIMV+PEEP group and PCV group(P<0.01 and P<0.05).Conclusions: All three kinds of mechanical ventilation modes can improve the hypoxia in elderly patients with severe thoracic injury complicated with ARDS.The CPAP+PSV mode has advantage over the another two ventilation modes in reducing the peak inspiratory pressure,which is more suitable for the treatment of flail chest.
2018, 43(2): 210-212.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.020
Abstract:
Objective: To compare the clinical effects between Jack dilator kyphoplasty(DKP) and balloon kyphoplasty(BKP) in the treatment of osteoporotic vertebral compression fractures.Methods: Fifty-two patients with osteoporotic vertebral compression fractures were divided into the DKP group(25 cases) and BKP group(27 cases) according to the treatment method.The operation effect between two groups was compared.Results: The differences of the operation time,peroperative bleeding and bone cement injection volume between two groups were not statistically significant(P>0.05).Compared with before treatment,the height of fanterior edge and middle part in two groups increased after operation and at last follow-up,and the Cobb angle in two groups after operation decreased compared with before operation(P<0.05 to P<0.01).The height of fanterior edge and Cobb angle in DKP group at last follow-up were higher and lower than that in BKP group,respectively(P<0.05).Compared with before operation,the scores of VAS and ODI in two groups decreased after operation and at last follow-up(P<0.05 to P<0.01),and the difference of which between two groups was not statistically significant(P>0.05).The bone cement leakage rate in DKP group was lower than that in BKP group(P<0.05).Conclusions: The effects of DKP and BKP in the treatment of osteoporotic vertebral compression fractures are similar,and the effect of DKP on the recovery of the convex Cobb angle and anterior height of the vertebral body is better than that of the BKP.
Objective: To compare the clinical effects between Jack dilator kyphoplasty(DKP) and balloon kyphoplasty(BKP) in the treatment of osteoporotic vertebral compression fractures.Methods: Fifty-two patients with osteoporotic vertebral compression fractures were divided into the DKP group(25 cases) and BKP group(27 cases) according to the treatment method.The operation effect between two groups was compared.Results: The differences of the operation time,peroperative bleeding and bone cement injection volume between two groups were not statistically significant(P>0.05).Compared with before treatment,the height of fanterior edge and middle part in two groups increased after operation and at last follow-up,and the Cobb angle in two groups after operation decreased compared with before operation(P<0.05 to P<0.01).The height of fanterior edge and Cobb angle in DKP group at last follow-up were higher and lower than that in BKP group,respectively(P<0.05).Compared with before operation,the scores of VAS and ODI in two groups decreased after operation and at last follow-up(P<0.05 to P<0.01),and the difference of which between two groups was not statistically significant(P>0.05).The bone cement leakage rate in DKP group was lower than that in BKP group(P<0.05).Conclusions: The effects of DKP and BKP in the treatment of osteoporotic vertebral compression fractures are similar,and the effect of DKP on the recovery of the convex Cobb angle and anterior height of the vertebral body is better than that of the BKP.
2018, 43(2): 213-215.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.021
Abstract:
Objective: To compare the short and long-term effects between retroperitoneal laparoscopic nephron-sparing surgery(NSS) and radical nephrectomy(RN) in the treatment of stage T1b renal carcinoma.Methods: Sixty stage T1b renal carcinoma patients treated with retroperitoneal laparoscopic surgery were divided into the NSS group(30 cases) and RN group(30 cases) according to the method of surgery.The operation time,peroperative bleeding,postoperative drainage time,fast time and hospital stay between two groups were compared.All patients were followed up for 2 years.The serum levels of Scr and BUN between two groups before operation and after 24 h,1 year and 2 years of operation were compared,and the 1-and 2-year survival rate between two groups were compared.Results: Compared with before operation,the serum levels of BUN and Scr in two groups after operation significantly increased(P<0.01),and the difference of which between two groups was not statistically significant(P>0.05).The serum levels of Scr and BUN in NSS group after 1 year and 2 years of operation were significantly lower than those in RN group(P<0.01).The differences of the operation time,peroperative bleeding,postoperative drainage time,fast time and hospital stay between two groups were not statistically significant(P>0.05).The differences of the 1-and 2-year survival rate between two groups were not statistically significant(P>0.05).Conclusions: The curative effect of NSS is similar to the RN.The influence of NSS on the long-term renal function is less,which can effectively protect renal function.
Objective: To compare the short and long-term effects between retroperitoneal laparoscopic nephron-sparing surgery(NSS) and radical nephrectomy(RN) in the treatment of stage T1b renal carcinoma.Methods: Sixty stage T1b renal carcinoma patients treated with retroperitoneal laparoscopic surgery were divided into the NSS group(30 cases) and RN group(30 cases) according to the method of surgery.The operation time,peroperative bleeding,postoperative drainage time,fast time and hospital stay between two groups were compared.All patients were followed up for 2 years.The serum levels of Scr and BUN between two groups before operation and after 24 h,1 year and 2 years of operation were compared,and the 1-and 2-year survival rate between two groups were compared.Results: Compared with before operation,the serum levels of BUN and Scr in two groups after operation significantly increased(P<0.01),and the difference of which between two groups was not statistically significant(P>0.05).The serum levels of Scr and BUN in NSS group after 1 year and 2 years of operation were significantly lower than those in RN group(P<0.01).The differences of the operation time,peroperative bleeding,postoperative drainage time,fast time and hospital stay between two groups were not statistically significant(P>0.05).The differences of the 1-and 2-year survival rate between two groups were not statistically significant(P>0.05).Conclusions: The curative effect of NSS is similar to the RN.The influence of NSS on the long-term renal function is less,which can effectively protect renal function.
2018, 43(2): 216-218,222.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.022
Abstract:
Objective: To analyze the related factors of complications in patients treated with laparoscopic pancreaticoduodenectomy(LPD).Methods: The incidence rate of complications and risk factors of total complications,pancreatic fistula and postoperative bleeding in 240 patients treated with LPD were analyzed.Results: The complication occurrence in 97 cases was found,which included 32 cases of pancreatic fistula(32.99%),49 cases of postoperative bleeding(50.52%),4 cases of incision liquefaction(4.12%) and 12 cases of infection(12.37%).The results of logistic regression analysis showed that the age ≥ 65 years and bleeding volume ≥ 200 mL were the risk factors of influencing the total complications,the age ≥ 65 years and pancreatic duct diameter ≤ 3 mm were the risk factors of pancreatic fistula,and the age ≥ 65 years was the risk factor of influencing postoperative bleeding of patients.Conclusions: The risk factors influencing complication in patients treated with LPD is more.During the perioperative period,the related indicators should be closely paid attention to and monitored to prevent the incidence of complications.
Objective: To analyze the related factors of complications in patients treated with laparoscopic pancreaticoduodenectomy(LPD).Methods: The incidence rate of complications and risk factors of total complications,pancreatic fistula and postoperative bleeding in 240 patients treated with LPD were analyzed.Results: The complication occurrence in 97 cases was found,which included 32 cases of pancreatic fistula(32.99%),49 cases of postoperative bleeding(50.52%),4 cases of incision liquefaction(4.12%) and 12 cases of infection(12.37%).The results of logistic regression analysis showed that the age ≥ 65 years and bleeding volume ≥ 200 mL were the risk factors of influencing the total complications,the age ≥ 65 years and pancreatic duct diameter ≤ 3 mm were the risk factors of pancreatic fistula,and the age ≥ 65 years was the risk factor of influencing postoperative bleeding of patients.Conclusions: The risk factors influencing complication in patients treated with LPD is more.During the perioperative period,the related indicators should be closely paid attention to and monitored to prevent the incidence of complications.
2018, 43(2): 219-222.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.023
Abstract:
Objective: To investigate the value of glycosylated serum protein(GSP) in the prediction of the short-term prognosis in patients with traumatic fracture of lower limb.Methods: One hundred and twenty-nine patients with traumatic fracture of lower limb(fracture group) were subdivided into the effective group(104 cases) and invalid group(25 cases),and 60 healthy volunteers were set as the control group.The serum levels of the GSP,fasting blood glucose(FPG),2 h postprandial blood glucose(2hPG),glycosylated hemoglobin(HbA1c),high-sensitivity C-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and procalcitonin (PCT) in three groups were detected.The correlation between GSP and inflammatory mediator was analyzed using Spearman correlation coefficient.According to the critical value of GSP(≥ 400 μmol/L),129 patients with traumatic fracture of lower limb were divided into the GSP ≥ 400 μmol/L subgroup(45 cases) and GSP<400 μmol/L subgroup(84 cases),and the incidence rate of postoperative complications between two groups was compared.Results: The level of GSP in fracture group[(451.4 ±12.6)μmol/L] was significantly higher than that in control group[(159.7±10.8)μmol/L](P<0.01),and 3 items of glucose indicators in fracture group were higher than those in control group(P<0.01).The level of GSP in invalid group[(673.2±24.8)μmol/L] was significantly higher than that in effective group[(299.0±7.1)μmol/L](P<0.01),the level of HbA1c in invalid group was higher than that in effective group(P<0.01) and the differences of the levels of FPG and 2hPG between two groups were not statistically significant(P>0.05).The levels of inflammatory mediators in effective group and invalid group were significantly higher than those in control group(P<0.01),and the level of inflammatory mediators in invalid group was significantly higher than that in effective group(P<0.01).The level of GSP in fracture patient was positively correlated with the levels of hs-CRP,TNF-α,IL-6 and PCT(P<0.05).The incidence rate of postoperative complication in GSP ≥ 400 μmol/L group(24.44%) was significantly higher than that in GSP<400 μmol/L group(4.76%)(P<0.01).Conclusions: The change of GSP level in patients with traumatic fracture of lower limb is found,which is closely related to the levels of IL-6 and TNF-α and postoperative complications,and has certain clinical value in evaluating the prognosis.
Objective: To investigate the value of glycosylated serum protein(GSP) in the prediction of the short-term prognosis in patients with traumatic fracture of lower limb.Methods: One hundred and twenty-nine patients with traumatic fracture of lower limb(fracture group) were subdivided into the effective group(104 cases) and invalid group(25 cases),and 60 healthy volunteers were set as the control group.The serum levels of the GSP,fasting blood glucose(FPG),2 h postprandial blood glucose(2hPG),glycosylated hemoglobin(HbA1c),high-sensitivity C-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and procalcitonin (PCT) in three groups were detected.The correlation between GSP and inflammatory mediator was analyzed using Spearman correlation coefficient.According to the critical value of GSP(≥ 400 μmol/L),129 patients with traumatic fracture of lower limb were divided into the GSP ≥ 400 μmol/L subgroup(45 cases) and GSP<400 μmol/L subgroup(84 cases),and the incidence rate of postoperative complications between two groups was compared.Results: The level of GSP in fracture group[(451.4 ±12.6)μmol/L] was significantly higher than that in control group[(159.7±10.8)μmol/L](P<0.01),and 3 items of glucose indicators in fracture group were higher than those in control group(P<0.01).The level of GSP in invalid group[(673.2±24.8)μmol/L] was significantly higher than that in effective group[(299.0±7.1)μmol/L](P<0.01),the level of HbA1c in invalid group was higher than that in effective group(P<0.01) and the differences of the levels of FPG and 2hPG between two groups were not statistically significant(P>0.05).The levels of inflammatory mediators in effective group and invalid group were significantly higher than those in control group(P<0.01),and the level of inflammatory mediators in invalid group was significantly higher than that in effective group(P<0.01).The level of GSP in fracture patient was positively correlated with the levels of hs-CRP,TNF-α,IL-6 and PCT(P<0.05).The incidence rate of postoperative complication in GSP ≥ 400 μmol/L group(24.44%) was significantly higher than that in GSP<400 μmol/L group(4.76%)(P<0.01).Conclusions: The change of GSP level in patients with traumatic fracture of lower limb is found,which is closely related to the levels of IL-6 and TNF-α and postoperative complications,and has certain clinical value in evaluating the prognosis.
2018, 43(2): 223-226.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.024
Abstract:
Objective: To investigate the recurrence rate of gallstone complicated with common bile duct stones treated with laparoscopic cholecystectomy(LC) combined with T tube drainage(LCHTD),and its influencing factors.Methods: The clinical data of 319 gallstone complicated with common bile duct stones patients treated with LC combined with LCHTD were retrospectively analyzed.The recurrence rate of gallbladder stone after 1 to 5 years of operation was observed,and the risk factor of which was investigated.Results: The recurrence rates after 1,3 and 5 years of operation were 4.39%(14 cases),9.40%(30 cases)and 14.73% (47 cases),respectively.The results of non-conditional logistic regression analysis showed that the multiple stones,lithotripsy,common bile duct dilatation and gallbladder tube dilatation were the independent risk factors of postoperative recurrence of cholecystolithiasis after LC combined with LCHTD treatment(OR=1.721,1.582,1.404 and 1.381,respectively,P<0.05).Conclusions: The lithotripsy,multiple stone,dilatation of common bile duct and dilation of gallbladder tube are the independent risk factors of recurrence of cholecystolithiasis after LC combined with LCHTD treatment.
Objective: To investigate the recurrence rate of gallstone complicated with common bile duct stones treated with laparoscopic cholecystectomy(LC) combined with T tube drainage(LCHTD),and its influencing factors.Methods: The clinical data of 319 gallstone complicated with common bile duct stones patients treated with LC combined with LCHTD were retrospectively analyzed.The recurrence rate of gallbladder stone after 1 to 5 years of operation was observed,and the risk factor of which was investigated.Results: The recurrence rates after 1,3 and 5 years of operation were 4.39%(14 cases),9.40%(30 cases)and 14.73% (47 cases),respectively.The results of non-conditional logistic regression analysis showed that the multiple stones,lithotripsy,common bile duct dilatation and gallbladder tube dilatation were the independent risk factors of postoperative recurrence of cholecystolithiasis after LC combined with LCHTD treatment(OR=1.721,1.582,1.404 and 1.381,respectively,P<0.05).Conclusions: The lithotripsy,multiple stone,dilatation of common bile duct and dilation of gallbladder tube are the independent risk factors of recurrence of cholecystolithiasis after LC combined with LCHTD treatment.
2018, 43(2): 226-228.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.025
Abstract:
Objective: To explore the application effects of Surgisis in the deep tissue defect repair after horseshoe anal fistula.Methods: Sixty patients with horseshoe anal fistula were randomly divided into the observation group and control group(30 cases each group).The control group was treated with routine wound dressing.The observation group was treated with routine wound dressing at first,and then the Surgisis stent of skin island patch was implanted after wound granulation fresh.The cure rate,wound healing time,postoperative complications,VAS score and scare area between two groups were compared.The number of granulation tissue and VEGF expression after treatment were detected using HE staining and IHC.Results: Compared with the control group,the cure rate significantly decreased(P<0.01),the healing time significantly shortened,and the number of granulation tissue and VEGF expression after treatment significantly increased in observation group(P<0.01).The anal damage rate and VAS score in observation group were significantly lower than those in control group(P<0.01),and scare area in observation group was significantly less than that in control group(P<0.01).Conclusions: The application of Surgisis stent in postoperative deep tissue defect repair of horseshoe anal fistula can promote the wound capillary generation,improve the wound microcirculation and speed up the wound healing,which is worthy of clinical use.
Objective: To explore the application effects of Surgisis in the deep tissue defect repair after horseshoe anal fistula.Methods: Sixty patients with horseshoe anal fistula were randomly divided into the observation group and control group(30 cases each group).The control group was treated with routine wound dressing.The observation group was treated with routine wound dressing at first,and then the Surgisis stent of skin island patch was implanted after wound granulation fresh.The cure rate,wound healing time,postoperative complications,VAS score and scare area between two groups were compared.The number of granulation tissue and VEGF expression after treatment were detected using HE staining and IHC.Results: Compared with the control group,the cure rate significantly decreased(P<0.01),the healing time significantly shortened,and the number of granulation tissue and VEGF expression after treatment significantly increased in observation group(P<0.01).The anal damage rate and VAS score in observation group were significantly lower than those in control group(P<0.01),and scare area in observation group was significantly less than that in control group(P<0.01).Conclusions: The application of Surgisis stent in postoperative deep tissue defect repair of horseshoe anal fistula can promote the wound capillary generation,improve the wound microcirculation and speed up the wound healing,which is worthy of clinical use.
2018, 43(2): 229-231.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.026
Abstract:
Objective: To analyze the clinical effects of the different uses of oxytocin in preventing the postpartum hemorrhage in cesarean section.Methods: One hundred and fifty puerpera scheduled by cesarean section were divided into the A,B and C groups(50 cases each group)by random number table method.The group A was injected with 20 U oxytocin in myometrium,the group B was intravenously injected with 10 U oxytocin after injecting 10 U oxytocin into myometrium,and the group C was injected with 5 U oxytocin by Murphy's tube combined with intravenous injection with 10 U oxytocin.The placental separation time,blood loss during operation and after 24 hours of operation,adverse effect,blood pressure and heart rate in three groups were observed.Results: The mean blood loss during and after 24 h of operation,and placental separation time in group C were(244.1±26.1)mL,(87.4±13.9) mL and(1.6±0.4)min,respectively,which was less than that in group A and group B(P<0.01).The mean arterial pressure in three groups after 5 min of admission decreased compared with before admission(P<0.01),and began to increase after 10 min of admission(P<0.01).The heart rates in A,B and C groups after 10 min of admission gradually decreased in turn(P<0.05 to P<0.01).The differences of the incidence rates of adverse reaction between three groups were not statistically significant(P>0.05).Conclusions: The 5 U oxytocin injecting by Murphy's tube combined with intravenous injection with 10 U oxytocin can effectively reduce the blood loss and placental separation time,which has little effect on the blood pressure and heart rate,and is worthy of promotion.
Objective: To analyze the clinical effects of the different uses of oxytocin in preventing the postpartum hemorrhage in cesarean section.Methods: One hundred and fifty puerpera scheduled by cesarean section were divided into the A,B and C groups(50 cases each group)by random number table method.The group A was injected with 20 U oxytocin in myometrium,the group B was intravenously injected with 10 U oxytocin after injecting 10 U oxytocin into myometrium,and the group C was injected with 5 U oxytocin by Murphy's tube combined with intravenous injection with 10 U oxytocin.The placental separation time,blood loss during operation and after 24 hours of operation,adverse effect,blood pressure and heart rate in three groups were observed.Results: The mean blood loss during and after 24 h of operation,and placental separation time in group C were(244.1±26.1)mL,(87.4±13.9) mL and(1.6±0.4)min,respectively,which was less than that in group A and group B(P<0.01).The mean arterial pressure in three groups after 5 min of admission decreased compared with before admission(P<0.01),and began to increase after 10 min of admission(P<0.01).The heart rates in A,B and C groups after 10 min of admission gradually decreased in turn(P<0.05 to P<0.01).The differences of the incidence rates of adverse reaction between three groups were not statistically significant(P>0.05).Conclusions: The 5 U oxytocin injecting by Murphy's tube combined with intravenous injection with 10 U oxytocin can effectively reduce the blood loss and placental separation time,which has little effect on the blood pressure and heart rate,and is worthy of promotion.
2018, 43(2): 232-234.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.027
Abstract:
Objective: To investigate the clinical effects of the mini-implant anchorage in orthodontic treatment.Methods: Eighty-four patients with orthodontic treatment were randomly divided into the control group and observation group.The control group and observation group were treated with conventional method and mini-implant anchorage,respectively.Results: After treatment,the back tooth grinding displacement and upper incisor inclination difference in observation group were significantly less than those in control group(P<0.01),and the gnashing of teeth protruding length difference in observation group was significantly more than that in control group(P<0.01).After treatment,the measure value of the upper and lower of jaw and improvement of eigenvalue of upper and lower of jaw in observation group were better than those in control group(P<0.05 to P<0.01).The chewing efficiency and bite force in observation group were significantly better than those in control group(P<0.01).The incidence rates of soft tissue edema and discomfort in observation group were lower than those in control group(P<0.05 and P<0.01).Conclusions: The mini-implant anchorage in orthodontic treatment has important clinical value,the incidence rate of adverse reactions is low,and which is worthy of clinical promotion.
Objective: To investigate the clinical effects of the mini-implant anchorage in orthodontic treatment.Methods: Eighty-four patients with orthodontic treatment were randomly divided into the control group and observation group.The control group and observation group were treated with conventional method and mini-implant anchorage,respectively.Results: After treatment,the back tooth grinding displacement and upper incisor inclination difference in observation group were significantly less than those in control group(P<0.01),and the gnashing of teeth protruding length difference in observation group was significantly more than that in control group(P<0.01).After treatment,the measure value of the upper and lower of jaw and improvement of eigenvalue of upper and lower of jaw in observation group were better than those in control group(P<0.05 to P<0.01).The chewing efficiency and bite force in observation group were significantly better than those in control group(P<0.01).The incidence rates of soft tissue edema and discomfort in observation group were lower than those in control group(P<0.05 and P<0.01).Conclusions: The mini-implant anchorage in orthodontic treatment has important clinical value,the incidence rate of adverse reactions is low,and which is worthy of clinical promotion.
2018, 43(2): 235-236.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.028
Abstract:
Objective: To compare the effects between turmeric acne-removing liniment combined with erythromycin enteric-coated capsule and adapalene gel combined with erythromycin enteric-coated capsule in the treatment of the mild and moderate acne vulgaris.Methods: Sixty-two patients with mild or moderate acne vulgaris were divided into the group A and group B according to the single-blind randomized controlled trial(31 cases each group).The group A and group B were treated with turmeric acne-removing liniment combined with erythromycin enteric-coated capsule and adapalene gel combined with erythromycin enteric-coated capsules,twice daily,for 8 weeks.The skin lesion in two groups were observed after treatment.Results: The effective rates in group A and group B were 74.2% and 77.4%,respectively,the difference of which was not statistically significant(P>0.05).Conclusions: The treatment effect of Turmeric Acne-removing Liniment combined with Erythromycin Enteric-coated Capsule is significant and less adverse reaction,which is worthy of clinical application.
Objective: To compare the effects between turmeric acne-removing liniment combined with erythromycin enteric-coated capsule and adapalene gel combined with erythromycin enteric-coated capsule in the treatment of the mild and moderate acne vulgaris.Methods: Sixty-two patients with mild or moderate acne vulgaris were divided into the group A and group B according to the single-blind randomized controlled trial(31 cases each group).The group A and group B were treated with turmeric acne-removing liniment combined with erythromycin enteric-coated capsule and adapalene gel combined with erythromycin enteric-coated capsules,twice daily,for 8 weeks.The skin lesion in two groups were observed after treatment.Results: The effective rates in group A and group B were 74.2% and 77.4%,respectively,the difference of which was not statistically significant(P>0.05).Conclusions: The treatment effect of Turmeric Acne-removing Liniment combined with Erythromycin Enteric-coated Capsule is significant and less adverse reaction,which is worthy of clinical application.
2018, 43(2): 242-244.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.031
Abstract:
Objective: To compare the application value between transabdominal ultrasonography and transvaginal ultrasonography in the diagnosis of scar pregnancy(CSP) after cesarean.Methods: Seventy patients suspected with CSP were detected using transabdominal and transvaginal ultrasound before operation,and diagonised with pathology after operation.The diagnostic accuracy between two groups was compared.Results: Among 70 patients,the CSP in 58 cases(82.86%) were diagnosed by pathological examination,which included 36 cases of gestational sac type(62.07%),18 cases of heterogeneous masses(31.03%) and 4 cases of mixed type(6.90%).The diagnostic rate of CSP in transabdominal combined with transvaginal ultrasonography and transvaginal ultrasonography was significantly higher than that in transabdominal ultrasonography(P<0.05),The difference of the diagnostic rate of CSP between transabdominal combined with transvaginal ultrasonography and transvaginal ultrasonography was not statistically significant(P>0.05).The sensitivity and specificity of the transabdominal combined with transvaginal ultrasonography in the diagnosis of CSP in were significantly higher than those in transabdominal ultrasonography(P<0.01),but there was no statistical significance between transvaginal ultrasonography and transabdominal combined with transvaginal ultrasonography(P>0.05).The sensitivity and specificity of transvaginal ultrasonography in the diagnosis of CSP were higher than those in transabdominal ultrasonography(P<0.01 and P<0.05).Conclusions: In the clinical diagnosis of scar pregnancy after cesarean section,the transvaginal ultrasonography can effectively compensate the lack of transabdominal ultrasonography,which can clearly show the range,location,blood supply and myometrial thickness of scar pregnancy,and improve the diagnostic accuracy of scar pregnancy.
Objective: To compare the application value between transabdominal ultrasonography and transvaginal ultrasonography in the diagnosis of scar pregnancy(CSP) after cesarean.Methods: Seventy patients suspected with CSP were detected using transabdominal and transvaginal ultrasound before operation,and diagonised with pathology after operation.The diagnostic accuracy between two groups was compared.Results: Among 70 patients,the CSP in 58 cases(82.86%) were diagnosed by pathological examination,which included 36 cases of gestational sac type(62.07%),18 cases of heterogeneous masses(31.03%) and 4 cases of mixed type(6.90%).The diagnostic rate of CSP in transabdominal combined with transvaginal ultrasonography and transvaginal ultrasonography was significantly higher than that in transabdominal ultrasonography(P<0.05),The difference of the diagnostic rate of CSP between transabdominal combined with transvaginal ultrasonography and transvaginal ultrasonography was not statistically significant(P>0.05).The sensitivity and specificity of the transabdominal combined with transvaginal ultrasonography in the diagnosis of CSP in were significantly higher than those in transabdominal ultrasonography(P<0.01),but there was no statistical significance between transvaginal ultrasonography and transabdominal combined with transvaginal ultrasonography(P>0.05).The sensitivity and specificity of transvaginal ultrasonography in the diagnosis of CSP were higher than those in transabdominal ultrasonography(P<0.01 and P<0.05).Conclusions: In the clinical diagnosis of scar pregnancy after cesarean section,the transvaginal ultrasonography can effectively compensate the lack of transabdominal ultrasonography,which can clearly show the range,location,blood supply and myometrial thickness of scar pregnancy,and improve the diagnostic accuracy of scar pregnancy.
2018, 43(2): 245-248.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.032
Abstract:
Objective: To evaluate the application value of MRI fresh blood imaging(FBI) in lower extremity venous imaging.Methods: Forty patients with lower extremity venous lesion were examined using MRI FBI and lower vein angiography.The quality of imaging,anatomical display,arterial pollution in all cases between two methods were compared.Results: The diagnosis result coincidence rate of FBI quality of lower extremity venous was 92.50%,and the consistency of which was good(Kappa value=0.699).The diagnosis result coincidence rate of organize fake film score was 82.50%,and the consistency of which was good(Kappa value=0.632).The difference of the number of venous vessels between two methods was not statistically significant(P>0.05).Conclusions: The differences of the quality of imaging,anatomical display and arterial pollution between two methods are not statistically significant,which is worthy of application in the patients with hypersensitivity to diodone and renal insufficiency.
Objective: To evaluate the application value of MRI fresh blood imaging(FBI) in lower extremity venous imaging.Methods: Forty patients with lower extremity venous lesion were examined using MRI FBI and lower vein angiography.The quality of imaging,anatomical display,arterial pollution in all cases between two methods were compared.Results: The diagnosis result coincidence rate of FBI quality of lower extremity venous was 92.50%,and the consistency of which was good(Kappa value=0.699).The diagnosis result coincidence rate of organize fake film score was 82.50%,and the consistency of which was good(Kappa value=0.632).The difference of the number of venous vessels between two methods was not statistically significant(P>0.05).Conclusions: The differences of the quality of imaging,anatomical display and arterial pollution between two methods are not statistically significant,which is worthy of application in the patients with hypersensitivity to diodone and renal insufficiency.
2018, 43(2): 249-251.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.033
Abstract:
Objective: To explore the interventional diagnosis and treatment of lower digestive tract hemorrhage.Methods: Twenty-two patients with lower digestive tract hemorrhage were detected using the digital substrate angiography(DSA).The bleeding lesions were treated with superselective artery embolization or transcatheter infusion vasopressin.Results: Among 22 patients,19 cases were clearly diagnosed,the positive diagnosis rate of which was 86.4%(19/22).Jejunum bleeding in 9 cases,ileum bleeding in 5 cases and colon bleeding in 5 cases were found.The hemostasis in 18 cases was successfully completed,the success rate of treatment was 94.7%(18/19).All patients were followed up for 6 to 12 months,the rebleeding in 2 cases were identified,and no severe complication was found.Conclusions: The interventional radiology in the diagnosis and treatment of lower digestive tract hemorrhage is safe and effective,especially,the interventional therapy has unique clinical values.
Objective: To explore the interventional diagnosis and treatment of lower digestive tract hemorrhage.Methods: Twenty-two patients with lower digestive tract hemorrhage were detected using the digital substrate angiography(DSA).The bleeding lesions were treated with superselective artery embolization or transcatheter infusion vasopressin.Results: Among 22 patients,19 cases were clearly diagnosed,the positive diagnosis rate of which was 86.4%(19/22).Jejunum bleeding in 9 cases,ileum bleeding in 5 cases and colon bleeding in 5 cases were found.The hemostasis in 18 cases was successfully completed,the success rate of treatment was 94.7%(18/19).All patients were followed up for 6 to 12 months,the rebleeding in 2 cases were identified,and no severe complication was found.Conclusions: The interventional radiology in the diagnosis and treatment of lower digestive tract hemorrhage is safe and effective,especially,the interventional therapy has unique clinical values.
2018, 43(2): 252-255.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.034
Abstract:
Objective: To explore the teaching effects of dual-track teaching of lecture-based learning(LBL) combined with case-based learning(CBL) in the discipline of pharmacy administration.Methods: One hundred and twenty students major in pharmacy in grade 2013 were randomly divided into experimental group and control group(60 students each group).The experimental group was taught using the double-track teaching mode of LBL in combination with CBL,and the control group was taught using the traditional LBL teaching mode.The theory examination results between two groups were compared after teaching,and the teaching effects in two groups were evaluated using questionnaire.Results: The examination scores in experimental group were higher than that in control group(P<0.05).The survey results showed that the students in experimental group held a positive attitude to dual-track teaching of LBL combined with CBL,and the students thought it not only could promote the mastery of basic knowledge,but also enhance the abilities of analyzing and solving problems and mobilizing students' learning initiation.Conclusions: The dual-track teaching of LBL combined with CBL can improve the teaching effects of the discipline of pharmacy administration,which is worthy of promotion.
Objective: To explore the teaching effects of dual-track teaching of lecture-based learning(LBL) combined with case-based learning(CBL) in the discipline of pharmacy administration.Methods: One hundred and twenty students major in pharmacy in grade 2013 were randomly divided into experimental group and control group(60 students each group).The experimental group was taught using the double-track teaching mode of LBL in combination with CBL,and the control group was taught using the traditional LBL teaching mode.The theory examination results between two groups were compared after teaching,and the teaching effects in two groups were evaluated using questionnaire.Results: The examination scores in experimental group were higher than that in control group(P<0.05).The survey results showed that the students in experimental group held a positive attitude to dual-track teaching of LBL combined with CBL,and the students thought it not only could promote the mastery of basic knowledge,but also enhance the abilities of analyzing and solving problems and mobilizing students' learning initiation.Conclusions: The dual-track teaching of LBL combined with CBL can improve the teaching effects of the discipline of pharmacy administration,which is worthy of promotion.
2018, 43(2): 256-258.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.035
Abstract:
Objective: To investigate the application effects of the transfusion quality monitoring in clinical transfusion nursing.Methods: Two hundred and forty-eight patients from January 2015 to June 2016 and 240 patients from June 2012 to December 2014 were divided into the observation group and control group,respectively.The transfusion quality in observation group was monitored during whole nursing course according to the standard process,and the control group was operated according to the conventional transfusion process.The error rate,incidence rate of adverse reaction during the process of blood transfusion and satisfaction rate of patients between two groups were compared.The understanding of basic transfusion knowledge,basic skills and related laws in nurses between two group were compared.Results: The error rate and incidence rate of adverse reaction during the process of transfusion in observation group were lower than those in control group(P<0.01 and P<0.05),the satisfaction rate of patients in observation group was significantly better than that in control group(P<0.01),and the basic transfusion knowledge and basic skill of nurses in observation group were significantly better than those in control group(P<0.01).Conclusions: The quality monitoring during whole nursing course can strengthen the control of clinical blood transfusion process and key point,ensure the blood transfusion safety,and improve the satisfaction rate of patients.
Objective: To investigate the application effects of the transfusion quality monitoring in clinical transfusion nursing.Methods: Two hundred and forty-eight patients from January 2015 to June 2016 and 240 patients from June 2012 to December 2014 were divided into the observation group and control group,respectively.The transfusion quality in observation group was monitored during whole nursing course according to the standard process,and the control group was operated according to the conventional transfusion process.The error rate,incidence rate of adverse reaction during the process of blood transfusion and satisfaction rate of patients between two groups were compared.The understanding of basic transfusion knowledge,basic skills and related laws in nurses between two group were compared.Results: The error rate and incidence rate of adverse reaction during the process of transfusion in observation group were lower than those in control group(P<0.01 and P<0.05),the satisfaction rate of patients in observation group was significantly better than that in control group(P<0.01),and the basic transfusion knowledge and basic skill of nurses in observation group were significantly better than those in control group(P<0.01).Conclusions: The quality monitoring during whole nursing course can strengthen the control of clinical blood transfusion process and key point,ensure the blood transfusion safety,and improve the satisfaction rate of patients.
2018, 43(2): 259-261,264.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.036
Abstract:
Objective: To investigate the effects of visiting nursing intervention on the sleep state and incidence of acute cardiac events(ACE) in patients with coronary heart disease(CHD).Methods: A total of 100 patients with CHD were randomly divided into the control group and intervention group(50 cases each group).The control group was nursed with conventional discharge guidance,and the interventional group were nursed with visiting nursing intervention within 1 year after discharge,the cognition,psychology and behavior of patients were regularly nursed.The sleep state,incidence of ACE,rehospitalization rate and fatality rate in two groups were observed within 1 year after discharge.Results: The scores of self rating scale of sleep(SRSS) in intervention group after 3,6 and 12 months of discharge were significantly lower than that in control group(P<0.01).In intervention group,the difference of the sleep quality between before and after discharge was statistically significant(P<0.01),which after 3,6 and 12 months of discharge was significantly lower than that on the day of discharge and after 1 and 2 months of discharge,and which after 6 and 12 months of discharge was lower than that after 3 months of discharge(P<0.01 and P<0.05).The difference of the sleep quality in control group between before and after discharge was not statistical significance(P>0.05).Compared with the control group,the incidence rate of ACE and rehospitalization rate in interventional group significantly decreased(P<0.01),and the difference of fatality rate between two groups was not statistically significant(P>0.05).Within 1 year of discharge,the incidence of ACE,which included cardiac sudden death,acute heart failure,unstable angina pectoris,st-segment elevation myocardial infarction,non-st segment elevation myocardial infarction and arrhythmia,was recorded,and the difference of composition of ACE between two groups was not statistically significant(P>0.05).Conclusions: Visiting nursing intervention can contribute to culture the positive and healthy mentality in patients with CHD,promote the formation of good life interview,improve sleep and reduce the incidence of ACE.
Objective: To investigate the effects of visiting nursing intervention on the sleep state and incidence of acute cardiac events(ACE) in patients with coronary heart disease(CHD).Methods: A total of 100 patients with CHD were randomly divided into the control group and intervention group(50 cases each group).The control group was nursed with conventional discharge guidance,and the interventional group were nursed with visiting nursing intervention within 1 year after discharge,the cognition,psychology and behavior of patients were regularly nursed.The sleep state,incidence of ACE,rehospitalization rate and fatality rate in two groups were observed within 1 year after discharge.Results: The scores of self rating scale of sleep(SRSS) in intervention group after 3,6 and 12 months of discharge were significantly lower than that in control group(P<0.01).In intervention group,the difference of the sleep quality between before and after discharge was statistically significant(P<0.01),which after 3,6 and 12 months of discharge was significantly lower than that on the day of discharge and after 1 and 2 months of discharge,and which after 6 and 12 months of discharge was lower than that after 3 months of discharge(P<0.01 and P<0.05).The difference of the sleep quality in control group between before and after discharge was not statistical significance(P>0.05).Compared with the control group,the incidence rate of ACE and rehospitalization rate in interventional group significantly decreased(P<0.01),and the difference of fatality rate between two groups was not statistically significant(P>0.05).Within 1 year of discharge,the incidence of ACE,which included cardiac sudden death,acute heart failure,unstable angina pectoris,st-segment elevation myocardial infarction,non-st segment elevation myocardial infarction and arrhythmia,was recorded,and the difference of composition of ACE between two groups was not statistically significant(P>0.05).Conclusions: Visiting nursing intervention can contribute to culture the positive and healthy mentality in patients with CHD,promote the formation of good life interview,improve sleep and reduce the incidence of ACE.
2018, 43(2): 262-264.
doi: 10.13898/j.cnki.issn.1000-2200.2018.02.037
Abstract:
Objective: To investigate the clinical effects of health education path in the interventional treatment of aortic dissection.Methods: The aortic dissection patients treated with intervention method from October 2011 to May 2013(20 cases) and from June 2013 to December 2014(20 cases) were divided into the control group and observation group,respectively.The control group was nursed with the routine method,and the observation group was nursed with health education path combined with routine method.The anxiety in two groups between before and after nursing was compared,and the knowledge of the disease and nursing quality evaluation between two groups were compared.Results: The difference of the Zung Self-rating Anxiety Scale(SAS) score before operation between two groups was not statistically significant(P>0.05),but the SAS score in observation group after operation was significantly lower than that in control group(P<0.01).The knowledge of the disease in observation group was significantly better than that in control group(P<0.01).The score of nursing quality evaluation in observation group was significantly higher than that in control group(P<0.01).Conclusions: The health education path in the interventional treatment of aortic dissection can effectively improve the psychological status and knowledge of the disease of patients,enhance the quality of clinical care,and deepen the connotation of high quality care,which is worthy of promotion in clinic.
Objective: To investigate the clinical effects of health education path in the interventional treatment of aortic dissection.Methods: The aortic dissection patients treated with intervention method from October 2011 to May 2013(20 cases) and from June 2013 to December 2014(20 cases) were divided into the control group and observation group,respectively.The control group was nursed with the routine method,and the observation group was nursed with health education path combined with routine method.The anxiety in two groups between before and after nursing was compared,and the knowledge of the disease and nursing quality evaluation between two groups were compared.Results: The difference of the Zung Self-rating Anxiety Scale(SAS) score before operation between two groups was not statistically significant(P>0.05),but the SAS score in observation group after operation was significantly lower than that in control group(P<0.01).The knowledge of the disease in observation group was significantly better than that in control group(P<0.01).The score of nursing quality evaluation in observation group was significantly higher than that in control group(P<0.01).Conclusions: The health education path in the interventional treatment of aortic dissection can effectively improve the psychological status and knowledge of the disease of patients,enhance the quality of clinical care,and deepen the connotation of high quality care,which is worthy of promotion in clinic.
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