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2018 Vol. 43, No. 5
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2018, 43(5): 561-566,572.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.001
Abstract:
Objective: To explore the effects of ginsenoside-Rh2(GS-Rh2) on the sensitivity of gastric cancer cells treated with oxaliplatin(L-OHP).Methods: The side population(SP) cells of human gastric cancers cell line SGC-7901 was screened using flow cytometry(FCM),the proliferation ability of SP cells was detected using CCK-8 kit.The half inhibition concentration(IC50) of GS-Rh2 and L-OHP on SGC-7901 SP cells were detected using MTT assay.The changes of cell cycle and apoptosis rate of SP cells intervened by GS-Rh2 combined with low concentration of L-OHP were detected using FCM,and the level changes of Bax and Bcl-2 protein in SP cells intervened by GS-Rh2 combined with L-OHP were measured using Western-blotting.Results: The proliferation capacity of SGC-7901 SP cells was significantly higher than that of non side population(P<0.01).GS-Rh2 could enhance the sensitivity of the L-OHP on SGC-7901-SP cells,block the cell cycle in G1 phase(P>0.05).The protein expressions of Bax and Bcl-2 increased and decreased,respectively(P<0.01),which was negatively correlated with the GS-Rh2 concentration(P<0.05).Conclusions: With the increasing of GS-Rh2 concentration,the GS-Rh2 gradually increases the sensitivity of L-OHP on SGC-7901 SP cells.GS-Rh2 can be acted as a sensitizer of anti-gastric cancer drug.
Objective: To explore the effects of ginsenoside-Rh2(GS-Rh2) on the sensitivity of gastric cancer cells treated with oxaliplatin(L-OHP).Methods: The side population(SP) cells of human gastric cancers cell line SGC-7901 was screened using flow cytometry(FCM),the proliferation ability of SP cells was detected using CCK-8 kit.The half inhibition concentration(IC50) of GS-Rh2 and L-OHP on SGC-7901 SP cells were detected using MTT assay.The changes of cell cycle and apoptosis rate of SP cells intervened by GS-Rh2 combined with low concentration of L-OHP were detected using FCM,and the level changes of Bax and Bcl-2 protein in SP cells intervened by GS-Rh2 combined with L-OHP were measured using Western-blotting.Results: The proliferation capacity of SGC-7901 SP cells was significantly higher than that of non side population(P<0.01).GS-Rh2 could enhance the sensitivity of the L-OHP on SGC-7901-SP cells,block the cell cycle in G1 phase(P>0.05).The protein expressions of Bax and Bcl-2 increased and decreased,respectively(P<0.01),which was negatively correlated with the GS-Rh2 concentration(P<0.05).Conclusions: With the increasing of GS-Rh2 concentration,the GS-Rh2 gradually increases the sensitivity of L-OHP on SGC-7901 SP cells.GS-Rh2 can be acted as a sensitizer of anti-gastric cancer drug.
2018, 43(5): 567-572.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.002
Abstract:
Objective: To investigate the effects of magnolol-2-O-β-D-glucopyranoside(Mag-glu) on the proliferation,invasion and migration of human breast cancer cells,and its possible mechanism.Methods: The effects of Mag-glu on the proliferation of MCF-7 and MDA-MB-231 cells were detected using MTT assay.The effects of Mag-glu on the migration and invasion ability of human breast cancer cells were analyzed using the wound healing assay and transwell assay,respectively.The effects of Mag-glu on the expression levels of hypoxia-inducible factor 1α(HIF-1α),matrix metalloproteinase-9(MMP-9) and cyclooxygenase-2(COX-2) were detected using Western blotting.Results: Mag-glu could inhibit the proliferation of MCF-7 and MDA-MB-231 cells in a concentration-and time-dependent manner.The migration ability of MDA-MB-231 cells decreased significantly after 24 h of treatment with Mag-glu(P<0.01).Transwell assay results showed that cell migration and invasion abilities significantly decreased after 24 h and 48 h of treatment with different concentrations of Mag-glu.The expression levels of HIF-1α,MMP-9 and COX-2 down-regulated with the increasing of the concentration of Mag-glu.Conclusions: Mag-glu can inhibit the proliferation,invasion and migration of human breast cancer cells in vitro,the mechanism of which may be inhibiting the HIF-1α signaling pathway to lead to the down-regulated expression of COX-2 and MMP-9.
Objective: To investigate the effects of magnolol-2-O-β-D-glucopyranoside(Mag-glu) on the proliferation,invasion and migration of human breast cancer cells,and its possible mechanism.Methods: The effects of Mag-glu on the proliferation of MCF-7 and MDA-MB-231 cells were detected using MTT assay.The effects of Mag-glu on the migration and invasion ability of human breast cancer cells were analyzed using the wound healing assay and transwell assay,respectively.The effects of Mag-glu on the expression levels of hypoxia-inducible factor 1α(HIF-1α),matrix metalloproteinase-9(MMP-9) and cyclooxygenase-2(COX-2) were detected using Western blotting.Results: Mag-glu could inhibit the proliferation of MCF-7 and MDA-MB-231 cells in a concentration-and time-dependent manner.The migration ability of MDA-MB-231 cells decreased significantly after 24 h of treatment with Mag-glu(P<0.01).Transwell assay results showed that cell migration and invasion abilities significantly decreased after 24 h and 48 h of treatment with different concentrations of Mag-glu.The expression levels of HIF-1α,MMP-9 and COX-2 down-regulated with the increasing of the concentration of Mag-glu.Conclusions: Mag-glu can inhibit the proliferation,invasion and migration of human breast cancer cells in vitro,the mechanism of which may be inhibiting the HIF-1α signaling pathway to lead to the down-regulated expression of COX-2 and MMP-9.
2018, 43(5): 573-576.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.003
Abstract:
Objective: To establish the nonalcoholic fatty liver disease(NAFLD) model in C57BL/6 mice using D12492 high-fat feed,and provide the basis in studying the NAFLD.Methods: Forty healthy male C57BL/6 mice were randomly divided into the control group and model group(20 mice each group).The control group and model group were fed with the normal feed and high-fat feed,respectively.After 4,8,12 and 16 weeks of feed,the body weight,liver weight and liver tissue homogenate were observed,the serum levels of ALT,total cholesterol,low-density lipoprotein and liver triglyceride were detected,and the liver pathology examination was implemented.Results: The body weight after 4 weeks of feed and liver weight after 8 weeks of feed in model group were higher than those in control group,respectively(P<0.05 to P<0.01).Compared with the control group,the serum ALT level in model group after 8 weeks of feed increased(P<0.05 to P<0.01).The levels of serum total cholesterol and low-density lipoprotein in model group at each time-point were higher than those in control group(P<0.05 to P<0.01).Compared with the control group,the content of triglyceride in model group after 12 weeks of feed increased(P<0.05 and P<0.01).The liver tissue staining showed that the liver tissue steatosis,liver cells swelling,fat cavitation in cytoplasm and liver cells inflammatory change were identified in model group after 12-week of feed,and the liver cells inflammatory change aggravated and a large number of inflammatory cells infiltrated in model group after 16-week of feed.Conclusions: The NAFLD model induced by high fat-diet is an ideal animal model,which is easy to establish and repeatable,and can provide stable animal model for the study of mechanism and clinical treatment of NAFLD.
Objective: To establish the nonalcoholic fatty liver disease(NAFLD) model in C57BL/6 mice using D12492 high-fat feed,and provide the basis in studying the NAFLD.Methods: Forty healthy male C57BL/6 mice were randomly divided into the control group and model group(20 mice each group).The control group and model group were fed with the normal feed and high-fat feed,respectively.After 4,8,12 and 16 weeks of feed,the body weight,liver weight and liver tissue homogenate were observed,the serum levels of ALT,total cholesterol,low-density lipoprotein and liver triglyceride were detected,and the liver pathology examination was implemented.Results: The body weight after 4 weeks of feed and liver weight after 8 weeks of feed in model group were higher than those in control group,respectively(P<0.05 to P<0.01).Compared with the control group,the serum ALT level in model group after 8 weeks of feed increased(P<0.05 to P<0.01).The levels of serum total cholesterol and low-density lipoprotein in model group at each time-point were higher than those in control group(P<0.05 to P<0.01).Compared with the control group,the content of triglyceride in model group after 12 weeks of feed increased(P<0.05 and P<0.01).The liver tissue staining showed that the liver tissue steatosis,liver cells swelling,fat cavitation in cytoplasm and liver cells inflammatory change were identified in model group after 12-week of feed,and the liver cells inflammatory change aggravated and a large number of inflammatory cells infiltrated in model group after 16-week of feed.Conclusions: The NAFLD model induced by high fat-diet is an ideal animal model,which is easy to establish and repeatable,and can provide stable animal model for the study of mechanism and clinical treatment of NAFLD.
2018, 43(5): 577-580.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.004
Abstract:
Objective: To investigate the expression of miRNA-27b in placenta of preeclampsia patients,and the effects of Salvia miltiorrhiza on its expression.Methods: Twenty placental tissue samples from severe preeclampsia patients,20 placental tissue samples from mild preeclampsia and 20 placental tissue samples from healthy placental tissue were divided into the severe preeclampsia group,mild preeclampsia group and control group,respectively.The expression levels of miRNA-27b and leptin mRNA were detected using the real-time fluorescence quantitative PCR(qRT-PCR).The miRNA-27b was processed using transient transfection,and the leptin mRNA level and human sertoli cells HTR-8 apoptosis were detected using the qRT-PCR and flow cytometry,respectively.Results: The expression levels of miRNA-27b and leptin mRNA in severe preeclampsia group were higher than those in mild preeclampsia group and control group,and the expression levels of miRNA-27b and leptin mRNA in mild preeclampsia were higher than those in control group(P<0.05).The expression levels of miRNA-27b and leptin mRNA in miRNA-27b mimics group significantly increased compared with the Con group and NC group(P<0.05),and the expression level of miRNA-27b in miRNA-27b inhibitor group decreased compared with the Con group and NC group(P<0.05).The differences of the expression levels of miRNA-27b and leptin mRNA between Con group and NC group were not statistically significant(P>0.05).The apoptosis levels of HTR-8 cells in miRNA-27b mimics group and miRNA-27b inhibitor group were higher and lower than that in Con group and NC group,respectively(P<0.05).The expression levels of miRNA-27b and leptin mRNA in severe preeclampsia group and mild preeclampsia group decreased compared with before Salvia miltiorrhiza treatment(P<0.05).Conclusions: The expression levels of miRNA-27b and leptin mRNA in preeclampsia placenta increase,miRNA-27b can promote the apoptosis of sertoli cells.miRNA-27b plays a positive regulation to leptin,both may work together in the development of preeclampsia.Salvia miltiorrhiza can inhibit the expression of miRNA-27b and leptin in preeclampsia.
Objective: To investigate the expression of miRNA-27b in placenta of preeclampsia patients,and the effects of Salvia miltiorrhiza on its expression.Methods: Twenty placental tissue samples from severe preeclampsia patients,20 placental tissue samples from mild preeclampsia and 20 placental tissue samples from healthy placental tissue were divided into the severe preeclampsia group,mild preeclampsia group and control group,respectively.The expression levels of miRNA-27b and leptin mRNA were detected using the real-time fluorescence quantitative PCR(qRT-PCR).The miRNA-27b was processed using transient transfection,and the leptin mRNA level and human sertoli cells HTR-8 apoptosis were detected using the qRT-PCR and flow cytometry,respectively.Results: The expression levels of miRNA-27b and leptin mRNA in severe preeclampsia group were higher than those in mild preeclampsia group and control group,and the expression levels of miRNA-27b and leptin mRNA in mild preeclampsia were higher than those in control group(P<0.05).The expression levels of miRNA-27b and leptin mRNA in miRNA-27b mimics group significantly increased compared with the Con group and NC group(P<0.05),and the expression level of miRNA-27b in miRNA-27b inhibitor group decreased compared with the Con group and NC group(P<0.05).The differences of the expression levels of miRNA-27b and leptin mRNA between Con group and NC group were not statistically significant(P>0.05).The apoptosis levels of HTR-8 cells in miRNA-27b mimics group and miRNA-27b inhibitor group were higher and lower than that in Con group and NC group,respectively(P<0.05).The expression levels of miRNA-27b and leptin mRNA in severe preeclampsia group and mild preeclampsia group decreased compared with before Salvia miltiorrhiza treatment(P<0.05).Conclusions: The expression levels of miRNA-27b and leptin mRNA in preeclampsia placenta increase,miRNA-27b can promote the apoptosis of sertoli cells.miRNA-27b plays a positive regulation to leptin,both may work together in the development of preeclampsia.Salvia miltiorrhiza can inhibit the expression of miRNA-27b and leptin in preeclampsia.
2018, 43(5): 581-584.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.005
Abstract:
Objective: To investigate the regulation effects of dexmedetomidine on nerve function,cerebral oxygen metabolism and inflammatory factor in craniocerebral operation patients.Methods: Ninety-eight patients treated with cerebral surgery were divided into the control group and observation group according to the draw method(45 cases each group).The control group was treated with routine anesthesia,and the observation group was treated with dexmedetomidine additionally based on control group.The levels of postoperative neurological function[including neuron specificity enolization enzyme(NSE),myelin basic protein(MBP) and S100β],brain oxygen metabolism[including radial artery blood oxygen content(CjvO2),radial artery blood oxygen saturation(SjvO2),internal carotid artery blood oxygen content(CaO2)],inflammatory factors[interleukin-6(IL-6),interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α)],postoperative recovery and adverse reactions between two groups were compared.Results: Level of neurologic functional indicators in both groups before surgery exhibited no statistically significant difference(P>0.05).After surgery,the level of nerve function indicators increased in both groups,which in the observation group was significantly better than that in the control group(P<0.01).Cerebral oxygen metabolism in both groups before surgery exhibited no significant difference(P>0.05).After surgery,the level of cerebral oxygen metabolism in both groups was decreased,while that of the observation group decreased more(P<0.01).The level of inflammatory factors in both groups before surgery showed no statistically significant difference(P>0.05).After surgery,the level of inflammatory factors in both groups was decreased,which in the observation group was lower than that in the control group(P<0.01).The terms of wake time,tube removal time,mortality rate and adverse reaction rate in both groups showed no statistically significant difference(P>0.05).Conclusions: Dexmedetomidine can protect the nerve function,reduce the cerebral oxygen metabolism and inflammatory factors in patients treated with craniocerebral surgery.
Objective: To investigate the regulation effects of dexmedetomidine on nerve function,cerebral oxygen metabolism and inflammatory factor in craniocerebral operation patients.Methods: Ninety-eight patients treated with cerebral surgery were divided into the control group and observation group according to the draw method(45 cases each group).The control group was treated with routine anesthesia,and the observation group was treated with dexmedetomidine additionally based on control group.The levels of postoperative neurological function[including neuron specificity enolization enzyme(NSE),myelin basic protein(MBP) and S100β],brain oxygen metabolism[including radial artery blood oxygen content(CjvO2),radial artery blood oxygen saturation(SjvO2),internal carotid artery blood oxygen content(CaO2)],inflammatory factors[interleukin-6(IL-6),interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α)],postoperative recovery and adverse reactions between two groups were compared.Results: Level of neurologic functional indicators in both groups before surgery exhibited no statistically significant difference(P>0.05).After surgery,the level of nerve function indicators increased in both groups,which in the observation group was significantly better than that in the control group(P<0.01).Cerebral oxygen metabolism in both groups before surgery exhibited no significant difference(P>0.05).After surgery,the level of cerebral oxygen metabolism in both groups was decreased,while that of the observation group decreased more(P<0.01).The level of inflammatory factors in both groups before surgery showed no statistically significant difference(P>0.05).After surgery,the level of inflammatory factors in both groups was decreased,which in the observation group was lower than that in the control group(P<0.01).The terms of wake time,tube removal time,mortality rate and adverse reaction rate in both groups showed no statistically significant difference(P>0.05).Conclusions: Dexmedetomidine can protect the nerve function,reduce the cerebral oxygen metabolism and inflammatory factors in patients treated with craniocerebral surgery.
2018, 43(5): 585-588.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.006
Abstract:
Objective: To analyze the clinical effects of the urteroscopic lithotripsy(URL),flexible urteroscopic lithotripsy(fURL),URL combined with N-Trap,extracorporeal shock wave lithotripsy(ESWL),mini-percustaneous nephrolithotomy(mPCNL) and retroperitoneal laparoscopic urterotomy(RLU) in the treatment of upper ureteric stones for providing the reference in selecting the best treatment means of upper ureteric stones.Methods: The clinical data of 186 cases treated with URL,39 cases treated with fURL,149 cases treated with URL combined with N-Trap,131 cases treated with ESWL,58 cases treated with mPCNL and 35 cases treated with PLU were analyzed.Results: The success rates of URL,fURL,URL combined with N-Trap,ESWL,mPCNL and RLU in treating upper ureteric stones were 60.2%,92.3%,89.9%,93.9%,96.6% and 97.1%,respectively.The differences of the success rates of fURL,URL combined with N-Trap,ESWL,mPCNL and RLU in treating upper ureteric stones were not statistically significant(P>0.05),and which was significanly higher than that of URL(P<0.01).The URL,fURL and URL combined with N-Trap were not suitable for the patients with N-shape bending ureter or failure to access below ureteral calculi.The once success rate of ESWL in patients with stone diameter ≤ 10 mm was higher than that in patients with stone diameter >10 mm(P<0.01),and the ESWL was not suitable for patients with polyp or stricture below the ureteral calculi.Conclusions: The once success rate of single-use URL in treating upper ureteral calculi is low,and the fURL or URL combined with N-Trap can improve the success rate of lithotripsy.Selecting the minimal invasive method to treat upper ureteric stone should be based on the stone location,size,complicated with ureter diseases and ureteroscopy auxiliary equipment.
Objective: To analyze the clinical effects of the urteroscopic lithotripsy(URL),flexible urteroscopic lithotripsy(fURL),URL combined with N-Trap,extracorporeal shock wave lithotripsy(ESWL),mini-percustaneous nephrolithotomy(mPCNL) and retroperitoneal laparoscopic urterotomy(RLU) in the treatment of upper ureteric stones for providing the reference in selecting the best treatment means of upper ureteric stones.Methods: The clinical data of 186 cases treated with URL,39 cases treated with fURL,149 cases treated with URL combined with N-Trap,131 cases treated with ESWL,58 cases treated with mPCNL and 35 cases treated with PLU were analyzed.Results: The success rates of URL,fURL,URL combined with N-Trap,ESWL,mPCNL and RLU in treating upper ureteric stones were 60.2%,92.3%,89.9%,93.9%,96.6% and 97.1%,respectively.The differences of the success rates of fURL,URL combined with N-Trap,ESWL,mPCNL and RLU in treating upper ureteric stones were not statistically significant(P>0.05),and which was significanly higher than that of URL(P<0.01).The URL,fURL and URL combined with N-Trap were not suitable for the patients with N-shape bending ureter or failure to access below ureteral calculi.The once success rate of ESWL in patients with stone diameter ≤ 10 mm was higher than that in patients with stone diameter >10 mm(P<0.01),and the ESWL was not suitable for patients with polyp or stricture below the ureteral calculi.Conclusions: The once success rate of single-use URL in treating upper ureteral calculi is low,and the fURL or URL combined with N-Trap can improve the success rate of lithotripsy.Selecting the minimal invasive method to treat upper ureteric stone should be based on the stone location,size,complicated with ureter diseases and ureteroscopy auxiliary equipment.
2018, 43(5): 589-591.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.007
Abstract:
Objective: To investigate the clinical significance of the combined detection of cystatin C(CysC),homocysteine(Hcy),hypersensitivity C reactive protein(hs-CRP)and D-dimer(D-D) in patients with atherosclerotic cerebral infarction.Methods: The serum levels of CysC,Hcy,hs-CRP and D-D in 85 patients with atherosclerotic cerebral infarction(observation group) and 40 healthy people(control group) were detected and analyzed.Results: The serum levels of CysC,Hcy,hs-CRP and D-D in observation group were significantly higher than those in control group(P<0.01).The area under curve,sensitivity,specificity,positive predictive value,negative predictive value and Youden index of the four combined detectiong of CysC,Hcy,hs-CRP and D-D were 0.956,91.26%,63.32%,89.79%,66.37% and 0.78,respectively.The clinical value of the combined detection was better than that of single item(P<0.05).Conclusions: The serum levels of CysC,Hcy,hs-CRP and D-D are closely related to the progression degree of atherosclerotic cerebral infarction.The combined detection of four indicators has great value in early diagnosis and evaluating disease of atherosclerotic cerebral infarction.
Objective: To investigate the clinical significance of the combined detection of cystatin C(CysC),homocysteine(Hcy),hypersensitivity C reactive protein(hs-CRP)and D-dimer(D-D) in patients with atherosclerotic cerebral infarction.Methods: The serum levels of CysC,Hcy,hs-CRP and D-D in 85 patients with atherosclerotic cerebral infarction(observation group) and 40 healthy people(control group) were detected and analyzed.Results: The serum levels of CysC,Hcy,hs-CRP and D-D in observation group were significantly higher than those in control group(P<0.01).The area under curve,sensitivity,specificity,positive predictive value,negative predictive value and Youden index of the four combined detectiong of CysC,Hcy,hs-CRP and D-D were 0.956,91.26%,63.32%,89.79%,66.37% and 0.78,respectively.The clinical value of the combined detection was better than that of single item(P<0.05).Conclusions: The serum levels of CysC,Hcy,hs-CRP and D-D are closely related to the progression degree of atherosclerotic cerebral infarction.The combined detection of four indicators has great value in early diagnosis and evaluating disease of atherosclerotic cerebral infarction.
Study on the correlation between T-lymphocyte subgroup count of peripheral blood and malignant tumor
2018, 43(5): 592-595.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.008
Abstract:
Objective: To investigate the clinical significance of T-lymphocyte subgroup count of peripheral blood in malignant tumor.Methods: The amount of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ of peripheral blood in 80 malignant tumor patients and 100 healthy people were determined by flow cytometry.Results: Compared with healthy people,the amount of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ in patients with malignant tumor were significantly decreased(P<0.01).The results of subgroup analysis showed that the counts of CD3+ cells and CD4+ cells in malignant tumor patients decreased compared with control group(P<0.05 to P<0.01),the difference of the count of CD8+ cells between two groups was not statistically significant(P>0.05),and ratio of CD4+/CD8+ in lung cancer group was lower than that in control group(P<0.05).The difference of the parameter of malignant tumor patients between the first diagnosis group and progression group was not statistically significant(P>0.05).The amount of CD4+ cells in amale patients was lower than that in female patients(P<0.05).Conclusions: The imbalance of the counts of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ in malignant tumor patients are identified,which can be used to evaluate the immunity status of tumor patients,and provide a scientific evidence in immunotherapy of tumor.
Objective: To investigate the clinical significance of T-lymphocyte subgroup count of peripheral blood in malignant tumor.Methods: The amount of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ of peripheral blood in 80 malignant tumor patients and 100 healthy people were determined by flow cytometry.Results: Compared with healthy people,the amount of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ in patients with malignant tumor were significantly decreased(P<0.01).The results of subgroup analysis showed that the counts of CD3+ cells and CD4+ cells in malignant tumor patients decreased compared with control group(P<0.05 to P<0.01),the difference of the count of CD8+ cells between two groups was not statistically significant(P>0.05),and ratio of CD4+/CD8+ in lung cancer group was lower than that in control group(P<0.05).The difference of the parameter of malignant tumor patients between the first diagnosis group and progression group was not statistically significant(P>0.05).The amount of CD4+ cells in amale patients was lower than that in female patients(P<0.05).Conclusions: The imbalance of the counts of CD3+ cells,CD4+ cells,CD8+ cells and ratio of CD4+/CD8+ in malignant tumor patients are identified,which can be used to evaluate the immunity status of tumor patients,and provide a scientific evidence in immunotherapy of tumor.
2018, 43(5): 595-598.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.009
Abstract:
Objective: To explore the correlation between the serum level of neutrophil gelationase associated lipocalin(NGAL) in type 2 diabetes mellitus(T2DM) and early diabetic kidney disease(DKD).Methods: A total of 95 patients with T2DM were divided into the normal albuminuria group(NA group,UAER<20 μg/min,n=25),microalbuminuria group(MA group,20 μg/min ≤ UAER<200 μg/min,n=30),and clinical albuminuria group(CA group,UAER ≥ 200 μg/min,n=40),and 39 healthy volunteers were set as normal control group.The serum NGAL levels in all cases were measured by ELISA,and the correlations between NGAL and sCr,BUN,CysC and eGFR were analyzed.The sensitivity of serum NGAL in early diagnosis of DKD was evaluated using receiver operating characteristic(ROC) curve.Results: The levels of serum NGAL in NA,MA,and CA groups increased compared with control group(P<0.01),which in CA group was the highest[(185.65±61.76)ng/mL].Spearman analysis showed that the serum NGAL level was positively correlated with the levels of BUN,sCr and CysC (rs=0.395,0.408 and 0.257,respectively,P<0.05 to P<0.01),and the serum NGAL level was negatively correlated with the levels of eGFR and Alb,respectively(rs=-0.328 and -0.430,respectively,P<0.01).The UAER>20 μg/min was set as critical point,the area of serum NGAL and CysC under ROC curve in MA group and CA group were 0.675 and 0.789,respectively.Conclusions: The serum NGAL level is related to the levels of BUN,sCr,CysC and eGFR in early DKD patients,and the NGAL may be a sensitive indicator in the early diagnosis of DKD.
Objective: To explore the correlation between the serum level of neutrophil gelationase associated lipocalin(NGAL) in type 2 diabetes mellitus(T2DM) and early diabetic kidney disease(DKD).Methods: A total of 95 patients with T2DM were divided into the normal albuminuria group(NA group,UAER<20 μg/min,n=25),microalbuminuria group(MA group,20 μg/min ≤ UAER<200 μg/min,n=30),and clinical albuminuria group(CA group,UAER ≥ 200 μg/min,n=40),and 39 healthy volunteers were set as normal control group.The serum NGAL levels in all cases were measured by ELISA,and the correlations between NGAL and sCr,BUN,CysC and eGFR were analyzed.The sensitivity of serum NGAL in early diagnosis of DKD was evaluated using receiver operating characteristic(ROC) curve.Results: The levels of serum NGAL in NA,MA,and CA groups increased compared with control group(P<0.01),which in CA group was the highest[(185.65±61.76)ng/mL].Spearman analysis showed that the serum NGAL level was positively correlated with the levels of BUN,sCr and CysC (rs=0.395,0.408 and 0.257,respectively,P<0.05 to P<0.01),and the serum NGAL level was negatively correlated with the levels of eGFR and Alb,respectively(rs=-0.328 and -0.430,respectively,P<0.01).The UAER>20 μg/min was set as critical point,the area of serum NGAL and CysC under ROC curve in MA group and CA group were 0.675 and 0.789,respectively.Conclusions: The serum NGAL level is related to the levels of BUN,sCr,CysC and eGFR in early DKD patients,and the NGAL may be a sensitive indicator in the early diagnosis of DKD.
2018, 43(5): 599-602.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.010
Abstract:
Objective: To study the monitoring methods,characteristics and risk factors of dysglycemia in preterm infants.Methods: The level of blood glucose in preterm infants was monitored and analyzed using the venous serum glucose determination and capillary whole blood glucose measurement.All preterm infants were intravenously injected with glucose,the capillary whole blood glucose in heel was dynamically monitored using blood glucose meter,and the concentration and velocity of glucose infusion were adjusted according to the monitoring results until the level of blood glucose was stable.The clinical data of all cases were retrospectively analyzed,and the characteristics and risk factors of dysglycemia were analyzed.Results: Among 118 preterm infants,the abnormal blood glucose rate,incidence rates of dysglycemia,hypoglycemia and hyperglycemia were 50.8%,49.2% and 1.69%,respectively.The low birth weight and asphyxia of newborn in preterm infants were the risk factors of venous hypoglycemia and peripheral blood hyperglycemia(P<0.05 to P<0.01).The cesarean delivery,low birth weight,twin pregnancy and asphyxia of newborn were the independent risk factors in peripheral blood hypoglycemia of preterm infants(P<0.05 to P<0.01).Conclusions: The incidence rate of dysglycemia in preterm infants is high,which should be actively monitored.Positive controlling the low birth weight and asphyxia of newborn is an important measure in reducing the abnormal glucose in preterm infants.
Objective: To study the monitoring methods,characteristics and risk factors of dysglycemia in preterm infants.Methods: The level of blood glucose in preterm infants was monitored and analyzed using the venous serum glucose determination and capillary whole blood glucose measurement.All preterm infants were intravenously injected with glucose,the capillary whole blood glucose in heel was dynamically monitored using blood glucose meter,and the concentration and velocity of glucose infusion were adjusted according to the monitoring results until the level of blood glucose was stable.The clinical data of all cases were retrospectively analyzed,and the characteristics and risk factors of dysglycemia were analyzed.Results: Among 118 preterm infants,the abnormal blood glucose rate,incidence rates of dysglycemia,hypoglycemia and hyperglycemia were 50.8%,49.2% and 1.69%,respectively.The low birth weight and asphyxia of newborn in preterm infants were the risk factors of venous hypoglycemia and peripheral blood hyperglycemia(P<0.05 to P<0.01).The cesarean delivery,low birth weight,twin pregnancy and asphyxia of newborn were the independent risk factors in peripheral blood hypoglycemia of preterm infants(P<0.05 to P<0.01).Conclusions: The incidence rate of dysglycemia in preterm infants is high,which should be actively monitored.Positive controlling the low birth weight and asphyxia of newborn is an important measure in reducing the abnormal glucose in preterm infants.
2018, 43(5): 603-605.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.011
Abstract:
Objective: To research the pathogens distribution of children's respiratory tract infection in Lu'an city of Anhui province in order to provide the reference basis for clinical diagnosis and treatment.Methods: The IgM antibodies of 11 different pathogens in the serum specimens from 3 466 children with respiratory tract infection from June 2014 to May 2016 were detected using the indirect immunofluorescence,and the positive detection rate of which was statistically analyzed.Results: The positive detection rate of pathogen in children with one pathogen infection was 31.4% at least,the positive detection rate of Mycoplasma pneumoniae(MP) was the highest,and followed by Influenza B virus and Adenovirus.The positive detection rate of pathogen in children with two or more than two kinds of pathogens infection was 2.9%.The positive detection rate of pathogen in boy was higher than that in girl,and which in spring and winter season was higher than that in summer and autumn season.Conclusions: The detection rate of MP in respiratory tract infection in Lu'an city of Anhui province is the highest.The infection of MP mixed with other pathogens is normal in clinic.
Objective: To research the pathogens distribution of children's respiratory tract infection in Lu'an city of Anhui province in order to provide the reference basis for clinical diagnosis and treatment.Methods: The IgM antibodies of 11 different pathogens in the serum specimens from 3 466 children with respiratory tract infection from June 2014 to May 2016 were detected using the indirect immunofluorescence,and the positive detection rate of which was statistically analyzed.Results: The positive detection rate of pathogen in children with one pathogen infection was 31.4% at least,the positive detection rate of Mycoplasma pneumoniae(MP) was the highest,and followed by Influenza B virus and Adenovirus.The positive detection rate of pathogen in children with two or more than two kinds of pathogens infection was 2.9%.The positive detection rate of pathogen in boy was higher than that in girl,and which in spring and winter season was higher than that in summer and autumn season.Conclusions: The detection rate of MP in respiratory tract infection in Lu'an city of Anhui province is the highest.The infection of MP mixed with other pathogens is normal in clinic.
2018, 43(5): 606-607,610.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.012
Abstract:
Objective: To investigate the short and long-term efficacy of high dose dexamethasone combined with cyclosporine in the treatment of immune thrombocytopenia(ITP) in elderly patients.Methods: Twenty-four elderly patients with ITP were randomly divided into the control group(12 cases treated with 40 mg/d of dexamethasone for 4 d) and observation group(12 cases treated with 40 mg/d of dexamethasone for 4 d combined with 3 mg·kg-1·d-1 of cyclosporine).The platelet levels and adverse reactions in two groups were observed after 1 week,2 weeks and 1 month of treatment.Results: The differences of the levels of platelet before,and after 7 d of treatment between two groups were not statistically significant(P>0.05).After 14 days and 1 month of treatment,the levels of platelet in observation group were significantly higher than that in control group(P<0.01),and the differences of the levels of platelet at different time-points between two groups were statistically significant(P<0.01).After 7 d,14 d and 1 month of treatment,the levels of platelet in two groups were significantly higher than that before treatment(P<0.01),and the level of platelet in control group after 14 d of treatment was significantly lower than that after 7 d of treatment(P<0.01).The difference of the adverse reaction between two groups was not statistically significant(P>0.05).Conclusions: The dexamethasone combined with cyclosporine can improve the short-term and long-term efficacy of elderly patients with ITP,and the adverse reaction of which is minute.
Objective: To investigate the short and long-term efficacy of high dose dexamethasone combined with cyclosporine in the treatment of immune thrombocytopenia(ITP) in elderly patients.Methods: Twenty-four elderly patients with ITP were randomly divided into the control group(12 cases treated with 40 mg/d of dexamethasone for 4 d) and observation group(12 cases treated with 40 mg/d of dexamethasone for 4 d combined with 3 mg·kg-1·d-1 of cyclosporine).The platelet levels and adverse reactions in two groups were observed after 1 week,2 weeks and 1 month of treatment.Results: The differences of the levels of platelet before,and after 7 d of treatment between two groups were not statistically significant(P>0.05).After 14 days and 1 month of treatment,the levels of platelet in observation group were significantly higher than that in control group(P<0.01),and the differences of the levels of platelet at different time-points between two groups were statistically significant(P<0.01).After 7 d,14 d and 1 month of treatment,the levels of platelet in two groups were significantly higher than that before treatment(P<0.01),and the level of platelet in control group after 14 d of treatment was significantly lower than that after 7 d of treatment(P<0.01).The difference of the adverse reaction between two groups was not statistically significant(P>0.05).Conclusions: The dexamethasone combined with cyclosporine can improve the short-term and long-term efficacy of elderly patients with ITP,and the adverse reaction of which is minute.
2018, 43(5): 608-610.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.013
Abstract:
Objective: To explore the clinical effect of acupuncture combined with kerotherapy in the treatment of vertebral artery type cervical spondylopathy(VACS).Methods: Sixty patients with VACS were randomly divided into the observation group and control group(30 cases each group).The control group and observation group were treated with acupuncture and kerotherapy combined with acupuncture,respectively.The clinical efficacy and TCM syndrome score in two groups were observed after 2 courses of treatment.Results: After two courses of treatment,the cure rate in observation group(30.0%) was significantly higher than that in control group(6.7%)(P<0.01),and the TCM syndrome score in observation group was significantly higher than that in control group(P<0.01).Conclusions: Kerotherapy combined with acupuncture in treating VACS has good effect,which can relieve the pain of patients,and is superior to acupuncture treatment.
Objective: To explore the clinical effect of acupuncture combined with kerotherapy in the treatment of vertebral artery type cervical spondylopathy(VACS).Methods: Sixty patients with VACS were randomly divided into the observation group and control group(30 cases each group).The control group and observation group were treated with acupuncture and kerotherapy combined with acupuncture,respectively.The clinical efficacy and TCM syndrome score in two groups were observed after 2 courses of treatment.Results: After two courses of treatment,the cure rate in observation group(30.0%) was significantly higher than that in control group(6.7%)(P<0.01),and the TCM syndrome score in observation group was significantly higher than that in control group(P<0.01).Conclusions: Kerotherapy combined with acupuncture in treating VACS has good effect,which can relieve the pain of patients,and is superior to acupuncture treatment.
2018, 43(5): 611-613.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.014
Abstract:
Objective: To investigate the predictive value of the early glucolipid metabolism index and hypersensitive C reactive protein(hs-CRP) in the outcome of gestational diabetes mellitus(GDM) patients and neonates.Methods: The clinical data of 372 patients with GDM were retrospectively analyzed,and the patients were divided into the adverse pregnancy outcome group(53 cases) and normal pregnancy outcome group(319 cases).The early glucolipid metabolism indexes and level of hs-CRP between two groups were compared.The value of early glucolipid metabolism indexes and hs-CRP level in predicting the adverse pregnantcy outcome were analyzed using the ROC curve.Results: The levels of hs-CRP,LDL-C,TG,TC,and HbA1c in adverse pregnancy outcome group were significantly higher than those in normal pregnancy outcome group(P<0.01).ROC curve analysis showed that the tangent points of hs-CRP,LDL-C,fasting blood glucose,TG,TC and HbA1c to predict the adverse GDM pregnancy outcome were 5.03 mg/L,4.97 mmol/L,9.06 mmol/L,5.29 mmol/L,8.13 mmol/L and 7.89%,respectively.The areas under ROC curve of hs-CRP,LDL-C,fasting blood glucose,TG,TC,HbA1c were 0.843,0.635,0.647,0.826,0.609 and 0.819,respectively.Conclusions: The levels of serum hs-CRP and early glucolipid metabolism indexes in adverse pregnancy outcome patients with GDM significantly increase,which has a certain application value in the prediction of the GDM patients and neonatal outcomes.
Objective: To investigate the predictive value of the early glucolipid metabolism index and hypersensitive C reactive protein(hs-CRP) in the outcome of gestational diabetes mellitus(GDM) patients and neonates.Methods: The clinical data of 372 patients with GDM were retrospectively analyzed,and the patients were divided into the adverse pregnancy outcome group(53 cases) and normal pregnancy outcome group(319 cases).The early glucolipid metabolism indexes and level of hs-CRP between two groups were compared.The value of early glucolipid metabolism indexes and hs-CRP level in predicting the adverse pregnantcy outcome were analyzed using the ROC curve.Results: The levels of hs-CRP,LDL-C,TG,TC,and HbA1c in adverse pregnancy outcome group were significantly higher than those in normal pregnancy outcome group(P<0.01).ROC curve analysis showed that the tangent points of hs-CRP,LDL-C,fasting blood glucose,TG,TC and HbA1c to predict the adverse GDM pregnancy outcome were 5.03 mg/L,4.97 mmol/L,9.06 mmol/L,5.29 mmol/L,8.13 mmol/L and 7.89%,respectively.The areas under ROC curve of hs-CRP,LDL-C,fasting blood glucose,TG,TC,HbA1c were 0.843,0.635,0.647,0.826,0.609 and 0.819,respectively.Conclusions: The levels of serum hs-CRP and early glucolipid metabolism indexes in adverse pregnancy outcome patients with GDM significantly increase,which has a certain application value in the prediction of the GDM patients and neonatal outcomes.
2018, 43(5): 614-616,620.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.015
Abstract:
Objective: To investigate the expression level of breast cancer metastasis suppressor 1(BRMS1) mRNA in colorectal carcinoma,and its clinical significance.Methods: The expression of BRMS1 mRNA in 40 colorectal carcinoma tissue specimens and 20 normal colorectum tissue specimens were detected using RT-PCR method,and the correlation of which with clinicopathologic features was analyzed.Results: The expression level of BRMS1 mRNA in colorectal carcinoma tissue(0.420±0.133)was lower than that in normal colorectum tissue(0.836±0.102) (P<0.01).The expression level of BRMS1 mRNA in colorectal carcinoma was not associated with age,sex,degree of differentiation and infiltrative depth(P>0.05),and was associated with lymph node metastasis(P<0.01).Conclusions: The expression level of BRMS1 mRNA in colorectal carcinoma tissue is low,which may be related to the lymph node metastasis of colorectal carcinoma.
Objective: To investigate the expression level of breast cancer metastasis suppressor 1(BRMS1) mRNA in colorectal carcinoma,and its clinical significance.Methods: The expression of BRMS1 mRNA in 40 colorectal carcinoma tissue specimens and 20 normal colorectum tissue specimens were detected using RT-PCR method,and the correlation of which with clinicopathologic features was analyzed.Results: The expression level of BRMS1 mRNA in colorectal carcinoma tissue(0.420±0.133)was lower than that in normal colorectum tissue(0.836±0.102) (P<0.01).The expression level of BRMS1 mRNA in colorectal carcinoma was not associated with age,sex,degree of differentiation and infiltrative depth(P>0.05),and was associated with lymph node metastasis(P<0.01).Conclusions: The expression level of BRMS1 mRNA in colorectal carcinoma tissue is low,which may be related to the lymph node metastasis of colorectal carcinoma.
2018, 43(5): 617-620.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.016
Abstract:
Objective: To investigate the correlations between arrhythmia and heart rate variability(HRV),and postoperative cardiac function in children with congenital heart disease(CHD) before and after intervention therapy.Methods: Seventy-nine CHD children treated with interventional occlusion were divided into the atrial septal defect group(ASD group,26 cases),ventricular septal defect(VSD group,39 cases),and patent ductus arteriosus(PDA group,14 cases) according to the disease.The changes of arrhythmia and HRV between three groups were compared before and after interventional therapy,and the correlation between postoperative cardiac function and HRV time domain indexes was analyzed.Results: Among 79 cases,the different types of arrhythmia of 40 cases(50.63%) in 28 patients(35.44%) were detected,and the ratios of atrial premature beat(<30 times/h),room premature beat(Lown's level 1) and incomplete right bundle branch block were 32.50%,22.5% and 12.50%,respectively.After operation,54 cases(68.35%) in 17 patients(21.52%) with different types arrhythmia were detected,which increased compared with before operation(P<0.05).The detection rate of different types arrhythmia in VSD group was the highest(53.70%),which was significantly higher than that before operation(P<0.05).The SDANN in PDA group during the postoperative 24 h was significantly higher than that with before operation(P<0.05).The SDANN,R-R and SDNN in ASD and VSD group during the postoperative 24 h were significantly higher than those before operation(P<0.05 to P<0.01).The levels of SDANN,R-R and SDNN in children with grade Ⅰ and Ⅱ Killip cardiac function were significantly higher than those in children with grade Ⅲ and Ⅳ Killip cardiac function(P<0.01).The level of ASDNN in children with grade Ⅰ Killip cardiac function was significantly higher than that in children with grade Ⅲ and Ⅳ Killip cardiac function during the postoperative 24 h(P<0.05).The rMSSD in children with grade Ⅰ and Ⅱ Killip cardiac function were significantly higher than that in children with grade Ⅳ Killip cardiac function during(P<0.05).HRV time domain index was significantly positively correlated with cardiac function classification(P<0.05).Conclusions: After intervention treatment,the new arrhythmia and HRV elevation in CHD children may occur,which is related to the heart function of patients.
Objective: To investigate the correlations between arrhythmia and heart rate variability(HRV),and postoperative cardiac function in children with congenital heart disease(CHD) before and after intervention therapy.Methods: Seventy-nine CHD children treated with interventional occlusion were divided into the atrial septal defect group(ASD group,26 cases),ventricular septal defect(VSD group,39 cases),and patent ductus arteriosus(PDA group,14 cases) according to the disease.The changes of arrhythmia and HRV between three groups were compared before and after interventional therapy,and the correlation between postoperative cardiac function and HRV time domain indexes was analyzed.Results: Among 79 cases,the different types of arrhythmia of 40 cases(50.63%) in 28 patients(35.44%) were detected,and the ratios of atrial premature beat(<30 times/h),room premature beat(Lown's level 1) and incomplete right bundle branch block were 32.50%,22.5% and 12.50%,respectively.After operation,54 cases(68.35%) in 17 patients(21.52%) with different types arrhythmia were detected,which increased compared with before operation(P<0.05).The detection rate of different types arrhythmia in VSD group was the highest(53.70%),which was significantly higher than that before operation(P<0.05).The SDANN in PDA group during the postoperative 24 h was significantly higher than that with before operation(P<0.05).The SDANN,R-R and SDNN in ASD and VSD group during the postoperative 24 h were significantly higher than those before operation(P<0.05 to P<0.01).The levels of SDANN,R-R and SDNN in children with grade Ⅰ and Ⅱ Killip cardiac function were significantly higher than those in children with grade Ⅲ and Ⅳ Killip cardiac function(P<0.01).The level of ASDNN in children with grade Ⅰ Killip cardiac function was significantly higher than that in children with grade Ⅲ and Ⅳ Killip cardiac function during the postoperative 24 h(P<0.05).The rMSSD in children with grade Ⅰ and Ⅱ Killip cardiac function were significantly higher than that in children with grade Ⅳ Killip cardiac function during(P<0.05).HRV time domain index was significantly positively correlated with cardiac function classification(P<0.05).Conclusions: After intervention treatment,the new arrhythmia and HRV elevation in CHD children may occur,which is related to the heart function of patients.
2018, 43(5): 621-623.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.017
Abstract:
Objective: To observe the effects ziprasidone combined with psychosocial intervention in the treatment of schizophrenia in children and adolescents.Methods: Sixty children and adolescents with schizophrenia were randomly divided into the observation group and control group(30 cases each group).The control group were treated with ziprasidone,and the observation group were treated with ziprasidone combined with psychological intervention for 12 weeks.The curative effects,side reaction,self-knowledge,social dysfunction and recovery,and mind support status in two groups before treatment and after 12 weeks of treatment were evaluated using the positive and negative syndrome scale(PANSS),insight and treatment attitude questionnaire(ITAQ),social disability screening schedule(SDSS),and perceived social support scale(PSSS),respectively.Results: Before the intervention,the differences of the total score and subscale score of PANSS,and scores of ITAQ,PSSS and SDSS between two groups were not statistically significant(P>0.05).The total and subscale scores of PANSS in two groups after the intervention were significantly than lower those before intervention(P<0.01),and the decreasing degree of which in observation group was more than that in control group(P<0.05 to P<0.01).The scores of ITAQ and PSSS in observation group after intervention were significantly higher than those before intervention(P<0.01),the SDSS score in observation group after intervention was significantly lower than that before intervention(P<0.01),and the differences of the scores of ITAQ,PSSS and SDSS in control group between before and after intervention were not statistically significant(P>0.05).Conclusions: The treatment of schizophrenia with ziprasidone combined with psychosocial intervention in children and adolescents can recover the self-knowledge,strengthen the social support system,and improve the social function,which is good treatment compliance and overall effect.
Objective: To observe the effects ziprasidone combined with psychosocial intervention in the treatment of schizophrenia in children and adolescents.Methods: Sixty children and adolescents with schizophrenia were randomly divided into the observation group and control group(30 cases each group).The control group were treated with ziprasidone,and the observation group were treated with ziprasidone combined with psychological intervention for 12 weeks.The curative effects,side reaction,self-knowledge,social dysfunction and recovery,and mind support status in two groups before treatment and after 12 weeks of treatment were evaluated using the positive and negative syndrome scale(PANSS),insight and treatment attitude questionnaire(ITAQ),social disability screening schedule(SDSS),and perceived social support scale(PSSS),respectively.Results: Before the intervention,the differences of the total score and subscale score of PANSS,and scores of ITAQ,PSSS and SDSS between two groups were not statistically significant(P>0.05).The total and subscale scores of PANSS in two groups after the intervention were significantly than lower those before intervention(P<0.01),and the decreasing degree of which in observation group was more than that in control group(P<0.05 to P<0.01).The scores of ITAQ and PSSS in observation group after intervention were significantly higher than those before intervention(P<0.01),the SDSS score in observation group after intervention was significantly lower than that before intervention(P<0.01),and the differences of the scores of ITAQ,PSSS and SDSS in control group between before and after intervention were not statistically significant(P>0.05).Conclusions: The treatment of schizophrenia with ziprasidone combined with psychosocial intervention in children and adolescents can recover the self-knowledge,strengthen the social support system,and improve the social function,which is good treatment compliance and overall effect.
2018, 43(5): 624-628.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.018
Abstract:
Objective: To explore the clinical effects of the posterior pedicle fixation of different segments in the treatment of severe thoracolumbar spine fractures.Methods: One hundred and twenty-seven severe thoracolumbar spine fractures patients treated with posterior pedicle fixation in 2016 were divided into the short segment group(67 cases),long segment group(35 cases),and injured vertebral segment group(25 cases) according to the different fixed segments.The operation situation in three groups were observed.Three groups were followed up for 1 to 2 years,and the clinical effects among three groups were compared.Results: The operation time and intraoperative blood loss in long segment group,injured vertebral segment group and short segment group gradually decreased(P<0.01),and the postoperative bed time in short segment group,injured vertebral segment group and long segment group gradually decreased(P<0.01).The differences of the postoperative anterior border height percentage of injured vertebrae and recovery of Cobb angle between long segment group and injured vertebral segment group were not statistically significant(P>0.05),and which was better than that in short segment group(P<0.01).The differences of the Oswestry Dability Index scores among three groups were not statistically significant(P>0.05).No serious adverse reaction occurred in three groups.Conclusions: The short segment fixation treatment has shorter operation time,and little intraoperative blood loss.The advantage of long segment fixation and the injured vertebral short segment fixation in the reset and ensuring the injured vertebral anterior edge height are more obvious.
Objective: To explore the clinical effects of the posterior pedicle fixation of different segments in the treatment of severe thoracolumbar spine fractures.Methods: One hundred and twenty-seven severe thoracolumbar spine fractures patients treated with posterior pedicle fixation in 2016 were divided into the short segment group(67 cases),long segment group(35 cases),and injured vertebral segment group(25 cases) according to the different fixed segments.The operation situation in three groups were observed.Three groups were followed up for 1 to 2 years,and the clinical effects among three groups were compared.Results: The operation time and intraoperative blood loss in long segment group,injured vertebral segment group and short segment group gradually decreased(P<0.01),and the postoperative bed time in short segment group,injured vertebral segment group and long segment group gradually decreased(P<0.01).The differences of the postoperative anterior border height percentage of injured vertebrae and recovery of Cobb angle between long segment group and injured vertebral segment group were not statistically significant(P>0.05),and which was better than that in short segment group(P<0.01).The differences of the Oswestry Dability Index scores among three groups were not statistically significant(P>0.05).No serious adverse reaction occurred in three groups.Conclusions: The short segment fixation treatment has shorter operation time,and little intraoperative blood loss.The advantage of long segment fixation and the injured vertebral short segment fixation in the reset and ensuring the injured vertebral anterior edge height are more obvious.
2018, 43(5): 628-630.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.019
Abstract:
Objective: To investigate the clinical efficacy of thoracoscopy combined with internal fixation in the treatment of multiple rib fractures.Methods: The clinical data of 28 patients with multiple rib fractures were retrospectively analyzed,and the patients were divided into the surgical treatment group and conservative treatment group according to the therapy method(14 cases each group).The visual analog scale(VAS) score,time in bed,length of hospital stay and complications in two groups before and after treatment were recorded.Results: The VAS scores in two groups after treatment gradually decreased,and the VAS scores in surgical treatment group after 24 h,48 h and 72 h of treatment were lower than that in conservative treatment group(P<0.05 to P<0.01).The time in bed and length of hospital stay in surgical treatment group were significantly shorter than those in conservative treatment group,respectively(P<0.01).During the 6 months of following up,6 cases with complications in conservative treatment group were found,4 cases with pleural effusion were treated with closed drainage of pleural cavity,2 cases were severe thoracic deformity complicated with refractory chest pain,and the incidence rate of complication was 42.9%.No complication occurred in surgical treatment group.Conclusions: The thoracoscopy combined with internal fixation in the treatment of multiple rib fractures can shorten the recovery time,reduce the duration of hospital stay,relieve pain and reduce complications of patients,which is worthy of promotion in clinic.
Objective: To investigate the clinical efficacy of thoracoscopy combined with internal fixation in the treatment of multiple rib fractures.Methods: The clinical data of 28 patients with multiple rib fractures were retrospectively analyzed,and the patients were divided into the surgical treatment group and conservative treatment group according to the therapy method(14 cases each group).The visual analog scale(VAS) score,time in bed,length of hospital stay and complications in two groups before and after treatment were recorded.Results: The VAS scores in two groups after treatment gradually decreased,and the VAS scores in surgical treatment group after 24 h,48 h and 72 h of treatment were lower than that in conservative treatment group(P<0.05 to P<0.01).The time in bed and length of hospital stay in surgical treatment group were significantly shorter than those in conservative treatment group,respectively(P<0.01).During the 6 months of following up,6 cases with complications in conservative treatment group were found,4 cases with pleural effusion were treated with closed drainage of pleural cavity,2 cases were severe thoracic deformity complicated with refractory chest pain,and the incidence rate of complication was 42.9%.No complication occurred in surgical treatment group.Conclusions: The thoracoscopy combined with internal fixation in the treatment of multiple rib fractures can shorten the recovery time,reduce the duration of hospital stay,relieve pain and reduce complications of patients,which is worthy of promotion in clinic.
2018, 43(5): 633-636.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.021
Abstract:
Objective: To explore the diagnostic and differential diagnostic value of mammography of microcalcifications in benign and malignant breast disease for improving the accuracy of diagnosis.Methods: Thirty-five breast cancer patients complicated with microcifications diagnosed by mammography and 37 patients with breast benign disease were investigated.The number,density,intensity,morphology and distribution of microcalcification between benign and malignant breast disease were retrospectively analyzed.Results: There were statistical significances in the density,number,intensity,morphology and distribution of microcalcification between benign and malignant breast disease(P<0.05 to P<0.01).The ratio of hypodense microcalcifications in malignant breast disease was higher than that in benign breast disease(P<0.05).The ratio of microcalcification number more than or equal to 20 cases in malignant breast disease was higher than that in benign breast disease(P<0.05).The ratio of intensity ≥ 10/cm2 in malignant breast disease was higher than that in benign breast disease(P<0.01).The ratio of coarse particle microcalcification in benign breast disease was significantly higher than that in malignant breast disease,and 1 case in breast cancer group was found.The ratio of the dusty,branching,vermicular and pleomorphic microcalcifications in breast cancer were higher(P<0.01).Scattering distribution of microcalcification was only in benign breast disease,while the ratio of linear and segmental distribution of microcalcifications in breast cancer group was higher(P<0.01).Conclusions: The density,number,intensity,morphology and distribution of microcalcification has certain correlation with benign and malignant breast disease,and the mammography of microcalcification can preliminarily estimate the benign and malignant breast disease.
Objective: To explore the diagnostic and differential diagnostic value of mammography of microcalcifications in benign and malignant breast disease for improving the accuracy of diagnosis.Methods: Thirty-five breast cancer patients complicated with microcifications diagnosed by mammography and 37 patients with breast benign disease were investigated.The number,density,intensity,morphology and distribution of microcalcification between benign and malignant breast disease were retrospectively analyzed.Results: There were statistical significances in the density,number,intensity,morphology and distribution of microcalcification between benign and malignant breast disease(P<0.05 to P<0.01).The ratio of hypodense microcalcifications in malignant breast disease was higher than that in benign breast disease(P<0.05).The ratio of microcalcification number more than or equal to 20 cases in malignant breast disease was higher than that in benign breast disease(P<0.05).The ratio of intensity ≥ 10/cm2 in malignant breast disease was higher than that in benign breast disease(P<0.01).The ratio of coarse particle microcalcification in benign breast disease was significantly higher than that in malignant breast disease,and 1 case in breast cancer group was found.The ratio of the dusty,branching,vermicular and pleomorphic microcalcifications in breast cancer were higher(P<0.01).Scattering distribution of microcalcification was only in benign breast disease,while the ratio of linear and segmental distribution of microcalcifications in breast cancer group was higher(P<0.01).Conclusions: The density,number,intensity,morphology and distribution of microcalcification has certain correlation with benign and malignant breast disease,and the mammography of microcalcification can preliminarily estimate the benign and malignant breast disease.
2018, 43(5): 639-641,644.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.023
Abstract:
Objective: To explore the expression of Kif2a in male semen,and its effects on male infertility.Methods: Seventy semen specimens were collected and purified.The total RNA in all specimens was extracted,and transcripted into cDNA.The Kif2a mRNA was detected using fluorescence quantitative PCR.The samples were divided into abnomal semen group and normal group according to the semen parameters(including sperm survival,sperm motility and sperm density) according to the fifth edition of "Laboratory manual for human semen testing and treatment" of WHO.Results: The Kif2a mRNA expression level in normal sperm survival and sperm vitality group was 7.54 times of abnormal group,and the difference of which was statistically significant(P<0.05).The difference of the Kif2a mRNA expression level between normal and abnormal sperm density group was not statistically significant(P>0.05).Conclusions: The low sperm motility and survival rate may be due to the low expression of Kif2a in spermatogonial cells caused by sperm cells spindle failing to form,which affects the pregnancy.
Objective: To explore the expression of Kif2a in male semen,and its effects on male infertility.Methods: Seventy semen specimens were collected and purified.The total RNA in all specimens was extracted,and transcripted into cDNA.The Kif2a mRNA was detected using fluorescence quantitative PCR.The samples were divided into abnomal semen group and normal group according to the semen parameters(including sperm survival,sperm motility and sperm density) according to the fifth edition of "Laboratory manual for human semen testing and treatment" of WHO.Results: The Kif2a mRNA expression level in normal sperm survival and sperm vitality group was 7.54 times of abnormal group,and the difference of which was statistically significant(P<0.05).The difference of the Kif2a mRNA expression level between normal and abnormal sperm density group was not statistically significant(P>0.05).Conclusions: The low sperm motility and survival rate may be due to the low expression of Kif2a in spermatogonial cells caused by sperm cells spindle failing to form,which affects the pregnancy.
2018, 43(5): 642-644.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.024
Abstract:
Objective: To investigate the value of the serum IgA,ratios of IgA/C3 and IgA/C4 in the differential diagnosis of primary IgA nephropathy and non-IgA nephropathy.Methods: The serum levels of IgA,C3 and C4 in 64 IgA nephropathy patients and 67 non-IgA nephropathy patients were detected using turbidimetric immunoassay,the ratios of IgA/C3 and IgA/C4 were calculated,the ROC curve were established,and the area under the curve was calculated.The significance of the serum IgA,ratios of IgA/C3 and IgA/C4 in predicting the IgA nephropathy was evaluated.Results: The serum IgA level,ratios of IgA/C3 and IgA/C4 in patients with primary IgA nephropathy significantly increased compared with other nephrotic patients(P<0.01),and the area under the ROC curve of the IgA,ratios of IgA/C3 and IgA/C4 was 0.692,0.718 and 0.747,respectively.Conclusions: In the absence of pathological diagnosis of renal biopsy,the IgA,ratios of IgA/C3 and IgA/C4 can play an auxiliary role in the differential diagnosis of primary IgA nephropathy.
Objective: To investigate the value of the serum IgA,ratios of IgA/C3 and IgA/C4 in the differential diagnosis of primary IgA nephropathy and non-IgA nephropathy.Methods: The serum levels of IgA,C3 and C4 in 64 IgA nephropathy patients and 67 non-IgA nephropathy patients were detected using turbidimetric immunoassay,the ratios of IgA/C3 and IgA/C4 were calculated,the ROC curve were established,and the area under the curve was calculated.The significance of the serum IgA,ratios of IgA/C3 and IgA/C4 in predicting the IgA nephropathy was evaluated.Results: The serum IgA level,ratios of IgA/C3 and IgA/C4 in patients with primary IgA nephropathy significantly increased compared with other nephrotic patients(P<0.01),and the area under the ROC curve of the IgA,ratios of IgA/C3 and IgA/C4 was 0.692,0.718 and 0.747,respectively.Conclusions: In the absence of pathological diagnosis of renal biopsy,the IgA,ratios of IgA/C3 and IgA/C4 can play an auxiliary role in the differential diagnosis of primary IgA nephropathy.
2018, 43(5): 645-647.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.025
Abstract:
Objective: To investigate the value of the plasma level of myeloperoxidase(MPO) in healthy and unhealthy people.Methods: Nine hundred and ninety-eight related specimens were divided into the normal group(330 cases) and abnormal group(668 cases) according to the cardiovascular indexes(such as total cholesterol,blood lipid,blood pressure,blood routine and electrocardiogram) except the MPO.The MPO content in two groups was detected using ELISA kit,the MPO distribution was analyzed,and the detection method was evaluated.Results: The plasma MPO level was related to the gender,total cholesterol,blood pressure,blood lipid,blood routine and electrocardiogram.Conclusions: The establishment of the reference value of plasma MPO level can play an early warning role in abnormal cardiovascular state,and provide a reference for clinical application.
Objective: To investigate the value of the plasma level of myeloperoxidase(MPO) in healthy and unhealthy people.Methods: Nine hundred and ninety-eight related specimens were divided into the normal group(330 cases) and abnormal group(668 cases) according to the cardiovascular indexes(such as total cholesterol,blood lipid,blood pressure,blood routine and electrocardiogram) except the MPO.The MPO content in two groups was detected using ELISA kit,the MPO distribution was analyzed,and the detection method was evaluated.Results: The plasma MPO level was related to the gender,total cholesterol,blood pressure,blood lipid,blood routine and electrocardiogram.Conclusions: The establishment of the reference value of plasma MPO level can play an early warning role in abnormal cardiovascular state,and provide a reference for clinical application.
2018, 43(5): 648-650,654.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.026
Abstract:
Objective: To investigate the correlations between learning burnout,psychological capital and coping style in medical students.Methods: One thousand six hundred and ten medical students from Bengbu Medical College were investigated using the College Students Learning Burnout Questionnaire,Positive Psychological Capital Questionnaire and Simple Coping Style Questionnaire,and the correlations between learning burnout,psychological capital and coping style in medical students were analyzed.Results: The score of learning burnout in students with high scores of psychological capital and positive coping was lower than that in students with low scores of psychological capital and positive copying(P<0.01).The score of learning burnout in students with high score of positive coping was higher than that in students with low score of positive coping(P<0.01).The hope,resilience,self-efficacy and positive coping were negatively correlated with learning burnout(P<0.01),and the negative coping was positively correlated with learning burnout(P<0.01).The resilience,psychological capital,positive coping and hope were negatively correlated with feeling down(P<0.05 to P<0.01).The hope,resilience and self-efficacy were negatively correlated with misbehave(P<0.01),and the negative coping and optimism were positively correlated with misbehave(P<0.01 and P<0.05).The self-efficacy,psychological capital and negative coping were negatively correlated with low achievement(P<0.01).Conclusions: The psychological capital,resilience,hope,optimism,positive and negative coping are significantly correlated with learning burnout.Negative coping is a positive predictor of learning burnout,and the other factors can predict the learning burnout in reverse.
Objective: To investigate the correlations between learning burnout,psychological capital and coping style in medical students.Methods: One thousand six hundred and ten medical students from Bengbu Medical College were investigated using the College Students Learning Burnout Questionnaire,Positive Psychological Capital Questionnaire and Simple Coping Style Questionnaire,and the correlations between learning burnout,psychological capital and coping style in medical students were analyzed.Results: The score of learning burnout in students with high scores of psychological capital and positive coping was lower than that in students with low scores of psychological capital and positive copying(P<0.01).The score of learning burnout in students with high score of positive coping was higher than that in students with low score of positive coping(P<0.01).The hope,resilience,self-efficacy and positive coping were negatively correlated with learning burnout(P<0.01),and the negative coping was positively correlated with learning burnout(P<0.01).The resilience,psychological capital,positive coping and hope were negatively correlated with feeling down(P<0.05 to P<0.01).The hope,resilience and self-efficacy were negatively correlated with misbehave(P<0.01),and the negative coping and optimism were positively correlated with misbehave(P<0.01 and P<0.05).The self-efficacy,psychological capital and negative coping were negatively correlated with low achievement(P<0.01).Conclusions: The psychological capital,resilience,hope,optimism,positive and negative coping are significantly correlated with learning burnout.Negative coping is a positive predictor of learning burnout,and the other factors can predict the learning burnout in reverse.
2018, 43(5): 651-654.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.027
Abstract:
Objective: To analyze the characteristics and present situation of positive emotion,negative emotion and interpersonal trust in medical students,and their relationships.Methods: Three hundred and eighty-eight medical students from a medical college in Anhui province were selected using a stratified cluster sampling method,and the students were investigated using the "Interpersonal Trust Scale (IT)" and "positive and negative emotion scale" (PANAS).Results: There was no statistical significance in the scores of interpersonal trust and positive emotion between male students and female students(P>0.05),and the score of negative emotion in male student was higher than that in female student(P<0.05).The total scores of the interpersonal trust and positive emotion in students with falling in love were higher than those in students without falling in love,the difference of the score of the negative emotion between students with and without falling in love was not statistically significant(P>0.05).The difference of the academic performance in students with different scores of interpersonal trust and positive emotion was not statistically significant(P>0.05).The score of negative emotion in general,poor and very poor academic performance students(P<0.05 to P<0.01),and the differences of the scores of negative emotion between general,poor and very poor academic performance were not statistically significant(P>0.05).The total score of interpersonal trust was positively related to the score of positive emotion(P<0.01),and negatively related to the score of negative emotion in medical students(P<0.01).Conclusions: Enhancing interpersonal trust can promote the expression of positive emotion,and reduce the expression of negative emotion in medical students.
Objective: To analyze the characteristics and present situation of positive emotion,negative emotion and interpersonal trust in medical students,and their relationships.Methods: Three hundred and eighty-eight medical students from a medical college in Anhui province were selected using a stratified cluster sampling method,and the students were investigated using the "Interpersonal Trust Scale (IT)" and "positive and negative emotion scale" (PANAS).Results: There was no statistical significance in the scores of interpersonal trust and positive emotion between male students and female students(P>0.05),and the score of negative emotion in male student was higher than that in female student(P<0.05).The total scores of the interpersonal trust and positive emotion in students with falling in love were higher than those in students without falling in love,the difference of the score of the negative emotion between students with and without falling in love was not statistically significant(P>0.05).The difference of the academic performance in students with different scores of interpersonal trust and positive emotion was not statistically significant(P>0.05).The score of negative emotion in general,poor and very poor academic performance students(P<0.05 to P<0.01),and the differences of the scores of negative emotion between general,poor and very poor academic performance were not statistically significant(P>0.05).The total score of interpersonal trust was positively related to the score of positive emotion(P<0.01),and negatively related to the score of negative emotion in medical students(P<0.01).Conclusions: Enhancing interpersonal trust can promote the expression of positive emotion,and reduce the expression of negative emotion in medical students.
2018, 43(5): 655-657.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.028
Abstract:
Objective: To compare the teaching effects between CBL and PBL combined with literature in standardized training of neurologic physicians.Methods: Thirty neurology resident physicians from "The First Affiliated Hospital of Bengbu Medical College" were randomly divided into the control group and observation group(15 cases each group).The control group was taught with PBL combined with literature,and the observation group was taught with CBL combined with literature for 10 months.The content of course was selected from the seventh edition of the neurology textbook published by the People's Medical Publishing House.The clinical knowledge and research ability between two groups were compared after teaching.Results: The writing record,case analysis record,clinical operation skill and research ability in observation group were higher than those in control group(P<0.05 to P<0.01).Conclusions: The CBL combined with literature is more suitable for the teaching of the standardized training of neurologic physicians.
Objective: To compare the teaching effects between CBL and PBL combined with literature in standardized training of neurologic physicians.Methods: Thirty neurology resident physicians from "The First Affiliated Hospital of Bengbu Medical College" were randomly divided into the control group and observation group(15 cases each group).The control group was taught with PBL combined with literature,and the observation group was taught with CBL combined with literature for 10 months.The content of course was selected from the seventh edition of the neurology textbook published by the People's Medical Publishing House.The clinical knowledge and research ability between two groups were compared after teaching.Results: The writing record,case analysis record,clinical operation skill and research ability in observation group were higher than those in control group(P<0.05 to P<0.01).Conclusions: The CBL combined with literature is more suitable for the teaching of the standardized training of neurologic physicians.
2018, 43(5): 657-660.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.029
Abstract:
Objective: To investigate the current state of clinical nursing teachers' psychology capital and work engagement.Methods: Total 105 clinical nursing teachers from three level of first-class hospitals were recruited using the convenience sampling method by questionnaires.Results: The mean score of psychological capital of the clinical nursing teacher was 95.05±8.10,the mean score of the work was 67.15±9.10.Whether the hospital had pre job training, special training skills in the hospital, standardized training and qualification admittance, teaching case writing and PPT production had positive significance to psychological capital for work. The optimism dimension has the greatest contribution to the work.Conclusions: Hospital administrators should take reasonable measures to cultivate positive and optimistic attitude of clinical nursing teachers,so as to improve the effectiveness of their work engagement.
Objective: To investigate the current state of clinical nursing teachers' psychology capital and work engagement.Methods: Total 105 clinical nursing teachers from three level of first-class hospitals were recruited using the convenience sampling method by questionnaires.Results: The mean score of psychological capital of the clinical nursing teacher was 95.05±8.10,the mean score of the work was 67.15±9.10.Whether the hospital had pre job training, special training skills in the hospital, standardized training and qualification admittance, teaching case writing and PPT production had positive significance to psychological capital for work. The optimism dimension has the greatest contribution to the work.Conclusions: Hospital administrators should take reasonable measures to cultivate positive and optimistic attitude of clinical nursing teachers,so as to improve the effectiveness of their work engagement.
2018, 43(5): 661-663.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.030
Abstract:
Objective: To study the application effects of case-based learning(CBL) combined with standardized patients(SP) teaching model in the standardized training of gynecology and obstetrics residents.Methods: Sixty newly enrolled gynecology and obstetrics trainees were randomly divided into the two groups.The two groups were trained with SP combined with CBL and lecture-based learning(LBL) model,respectively.After 2 years of clinical training,the clinical comprehensive abilities in two groups were evaluated.The satisfaction of two teaching methods of trainees was investigated using questionnaire.Results: Compared with the LBL teaching model,the SP combined with CBL teaching model could significantly improve the clinical thinking ability,ability of communicating with patients and learning interest of trainees(P<0.01).Conclusions: The SP combined with CBL teaching model is practicable in the standardized training of obstetrics and gynecology residents,and has good teaching effect.
Objective: To study the application effects of case-based learning(CBL) combined with standardized patients(SP) teaching model in the standardized training of gynecology and obstetrics residents.Methods: Sixty newly enrolled gynecology and obstetrics trainees were randomly divided into the two groups.The two groups were trained with SP combined with CBL and lecture-based learning(LBL) model,respectively.After 2 years of clinical training,the clinical comprehensive abilities in two groups were evaluated.The satisfaction of two teaching methods of trainees was investigated using questionnaire.Results: Compared with the LBL teaching model,the SP combined with CBL teaching model could significantly improve the clinical thinking ability,ability of communicating with patients and learning interest of trainees(P<0.01).Conclusions: The SP combined with CBL teaching model is practicable in the standardized training of obstetrics and gynecology residents,and has good teaching effect.
2018, 43(5): 673-675.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.034
Abstract:
Objective: To explore the application effects of continuous quality improvement(CQI) in the prevention of complications caused by artificial liver therapy.Methods: The clinical data of 24 patients treated with artificial liver from October 2015 to December 2016 were retrospectively analyzed(control group),and the major complication of the patients was summarized.Twenty-four patients patients treated with artificial liver from April to December 2017 were randomly selected,and set as the observation group.The corresponding countermeasures to the related factors of complication in control group were formulated,and the CQI measurement was implemented in observation group.The incidence rates of infection,bleeding and hematoma,deep vein thrombosis and allergic reaction between two groups were compared.Results: The incidence rates of infection,allergic reaction and bleeding and hematoma in observation group,were lower than those in control group,respectively(P<0.05 to P<0.01).The difference of the incidence rate of deep vein thrombosis between the two groups was not statistically significant(P>0.05).Conclusions: Continuous quality improvement can reduce the incidence rate of complications caused by artificial liver therapy,and improve the therapeutic effects.
Objective: To explore the application effects of continuous quality improvement(CQI) in the prevention of complications caused by artificial liver therapy.Methods: The clinical data of 24 patients treated with artificial liver from October 2015 to December 2016 were retrospectively analyzed(control group),and the major complication of the patients was summarized.Twenty-four patients patients treated with artificial liver from April to December 2017 were randomly selected,and set as the observation group.The corresponding countermeasures to the related factors of complication in control group were formulated,and the CQI measurement was implemented in observation group.The incidence rates of infection,bleeding and hematoma,deep vein thrombosis and allergic reaction between two groups were compared.Results: The incidence rates of infection,allergic reaction and bleeding and hematoma in observation group,were lower than those in control group,respectively(P<0.05 to P<0.01).The difference of the incidence rate of deep vein thrombosis between the two groups was not statistically significant(P>0.05).Conclusions: Continuous quality improvement can reduce the incidence rate of complications caused by artificial liver therapy,and improve the therapeutic effects.
2018, 43(5): 676-679.
doi: 10.13898/j.cnki.issn.1000-2200.2018.05.035
Abstract:
Objective: To investigate the needs of elderly community home care services for providing the evidence for the relevant government department in formulating the corresponding measures to improve the elderly home care conditions.Methods: One hundred and eighty-nine elderly people from Shanghai Jing'an district north station street community were investigated using the self-designed"the Questionnaire on the Needs of Elderly Community Home Care Service" by a convenience sampling method.Results: The elderly service hotline and nursing home were the most and least demanding project(55.6% and 3.7%),respectively.Ten different projects in different ages elderly were demanded,and the demand rates of rehabilitation,home care,escort,visit hospital,day care,nursing home and legal aid gradually decreased with increasing of ages(P<0.05 to P<0.01).The demand rates of elderly service hotline in 60 to 70 years old group,more than or equal to 80 years old group and 70 to 80 years old group gradually decrease(P<0.01),the demand rate of daily shopping gradually increased with the increasing of age(P<0.05),and the demand rate of doing homework in 70 to 80 years old group was higher than that in other two groups(P<0.05).Seven different projects in elderly with different occupations were demanded,the demand rates of rehabilitation,home care,hospital home,elderly service hotline and legal aid in mental labor group were higher than those in physical labor group(P<0.05 to P<0.01),and the demand rates of chat and daily shopping in mental labor group were lower than those in physical labor group(P<0.05 and P<0.01).Five different projects in elderly with different living with children affected the demand,and the demand rates of rehabilitation,home care,visiting hospital,hospital home and elderly service hotline in the same village/community and city group were higher than those in other district group(P<0.05 to P<0.01).Conclusions: The elderly with different demographic sociology has different needs,and the relevant government departments should provide the corresponding service project according to actual needs.
Objective: To investigate the needs of elderly community home care services for providing the evidence for the relevant government department in formulating the corresponding measures to improve the elderly home care conditions.Methods: One hundred and eighty-nine elderly people from Shanghai Jing'an district north station street community were investigated using the self-designed"the Questionnaire on the Needs of Elderly Community Home Care Service" by a convenience sampling method.Results: The elderly service hotline and nursing home were the most and least demanding project(55.6% and 3.7%),respectively.Ten different projects in different ages elderly were demanded,and the demand rates of rehabilitation,home care,escort,visit hospital,day care,nursing home and legal aid gradually decreased with increasing of ages(P<0.05 to P<0.01).The demand rates of elderly service hotline in 60 to 70 years old group,more than or equal to 80 years old group and 70 to 80 years old group gradually decrease(P<0.01),the demand rate of daily shopping gradually increased with the increasing of age(P<0.05),and the demand rate of doing homework in 70 to 80 years old group was higher than that in other two groups(P<0.05).Seven different projects in elderly with different occupations were demanded,the demand rates of rehabilitation,home care,hospital home,elderly service hotline and legal aid in mental labor group were higher than those in physical labor group(P<0.05 to P<0.01),and the demand rates of chat and daily shopping in mental labor group were lower than those in physical labor group(P<0.05 and P<0.01).Five different projects in elderly with different living with children affected the demand,and the demand rates of rehabilitation,home care,visiting hospital,hospital home and elderly service hotline in the same village/community and city group were higher than those in other district group(P<0.05 to P<0.01).Conclusions: The elderly with different demographic sociology has different needs,and the relevant government departments should provide the corresponding service project according to actual needs.
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